LOCUS       NM_001365088            8072 bp    mRNA    linear   PRI 06-OCT-2024
DEFINITION  Homo sapiens solute carrier family 12 member 6 (SLC12A6),
            transcript variant 7, mRNA.
ACCESSION   NM_001365088
VERSION     NM_001365088.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8072)
  AUTHORS   Loseth,S., Hoyer,H., Le,K.M., Delpire,E., Kinge,E., Lande,A.,
            Hilmarsen,H.T., Fagerheim,T., Nilssen,O. and Braathen,G.J.
  TITLE     Late-onset sensory-motor axonal neuropathy, a novel SLC12A6-related
            phenotype
  JOURNAL   Brain 146 (3), 912-922 (2023)
   PUBMED   36542484
  REMARK    GeneRIF: Late-onset sensory-motor axonal neuropathy, a novel
            SLC12A6-related phenotype.
REFERENCE   2  (bases 1 to 8072)
  AUTHORS   Ando,M., Higuchi,Y., Yuan,J., Yoshimura,A., Taniguchi,T., Takei,J.,
            Takeuchi,M., Hiramatsu,Y., Shimizu,F., Kubota,M., Takeshima,A.,
            Ueda,T., Koh,K., Nagaoka,U., Tokashiki,T., Sawai,S., Sakiyama,Y.,
            Hashiguchi,A., Sato,R., Kanda,T., Okamoto,Y. and Takashima,H.
  TITLE     Novel heterozygous variants of SLC12A6 in Japanese families with
            Charcot-Marie-Tooth disease
  JOURNAL   Ann Clin Transl Neurol 9 (7), 902-911 (2022)
   PUBMED   35733399
  REMARK    GeneRIF: Novel heterozygous variants of SLC12A6 in Japanese
            families with Charcot-Marie-Tooth disease.
REFERENCE   3  (bases 1 to 8072)
  AUTHORS   Bogdanova-Mihaylova,P., McNamara,P., Burton-Jones,S. and
            Murphy,S.M.
  TITLE     Expanding the phenotype of SLC12A6-associated sensorimotor
            neuropathy
  JOURNAL   BMJ Case Rep 14 (10), e244641 (2021)
   PUBMED   34706912
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 8072)
  AUTHORS   Chi,G., Ebenhoch,R., Man,H., Tang,H., Tremblay,L.E., Reggiano,G.,
            Qiu,X., Bohstedt,T., Liko,I., Almeida,F.G., Garneau,A.P., Wang,D.,
            McKinley,G., Moreau,C.P., Bountra,K.D., Abrusci,P.,
            Mukhopadhyay,S.M.M., Fernandez-Cid,A., Slimani,S., Lavoie,J.L.,
            Burgess-Brown,N.A., Tehan,B., DiMaio,F., Jazayeri,A., Isenring,P.,
            Robinson,C.V. and Durr,K.L.
  TITLE     Phospho-regulation, nucleotide binding and ion access control in
            potassium-chloride cotransporters
  JOURNAL   EMBO J 40 (14), e107294 (2021)
   PUBMED   34031912
REFERENCE   5  (bases 1 to 8072)
  AUTHORS   Chakraborty,M., Asraf,H., Sekler,I. and Hershfinkel,M.
  TITLE     ZnR/GPR39 controls cell migration by orchestrating recruitment of
            KCC3 into protrusions, re-organization of actin and activation of
            MMP
  JOURNAL   Cell Calcium 94, 102330 (2021)
   PUBMED   33465674
  REMARK    GeneRIF: ZnR/GPR39 controls cell migration by orchestrating
            recruitment of KCC3 into protrusions, re-organization of actin and
            activation of MMP.
REFERENCE   6  (bases 1 to 8072)
  AUTHORS   Casula,S., Shmukler,B.E., Wilhelm,S., Stuart-Tilley,A.K., Su,W.,
            Chernova,M.N., Brugnara,C. and Alper,S.L.
  TITLE     A dominant negative mutant of the KCC1 K-Cl cotransporter: both N-
            and C-terminal cytoplasmic domains are required for K-Cl
            cotransport activity
  JOURNAL   J Biol Chem 276 (45), 41870-41878 (2001)
   PUBMED   11551954
REFERENCE   7  (bases 1 to 8072)
  AUTHORS   Race,J.E., Makhlouf,F.N., Logue,P.J., Wilson,F.H., Dunham,P.B. and
            Holtzman,E.J.
  TITLE     Molecular cloning and functional characterization of KCC3, a new
            K-Cl cotransporter
  JOURNAL   Am J Physiol 277 (6), C1210-C1219 (1999)
   PUBMED   10600773
REFERENCE   8  (bases 1 to 8072)
  AUTHORS   Mount,D.B., Mercado,A., Song,L., Xu,J., George,A.L. Jr., Delpire,E.
            and Gamba,G.
  TITLE     Cloning and characterization of KCC3 and KCC4, new members of the
            cation-chloride cotransporter gene family
  JOURNAL   J Biol Chem 274 (23), 16355-16362 (1999)
   PUBMED   10347194
REFERENCE   9  (bases 1 to 8072)
  AUTHORS   Hiki,K., D'Andrea,R.J., Furze,J., Crawford,J., Woollatt,E.,
            Sutherland,G.R., Vadas,M.A. and Gamble,J.R.
  TITLE     Cloning, characterization, and chromosomal location of a novel
            human K+-Cl- cotransporter
  JOURNAL   J Biol Chem 274 (15), 10661-10667 (1999)
   PUBMED   10187864
REFERENCE   10 (bases 1 to 8072)
  AUTHORS   Gauvreau,C., Brisson,J.D. and Dupre,N.
  TITLE     Hereditary Motor and Sensory Neuropathy with Agenesis of the Corpus
            Callosum
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301546
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BX648195.1, BC033894.1,
            AF477977.1, AF116242.1, BC051744.1 and AC079203.9.
            
            Summary: This gene is a member of the K-Cl cotransporter (KCC)
            family. K-Cl cotransporters are integral membrane proteins that
            lower intracellular chloride concentrations below the
            electrochemical equilibrium potential. The proteins encoded by this
            gene are activated by cell swelling induced by hypotonic
            conditions. Alternate splicing results in multiple transcript
            variants encoding different isoforms. Mutations in this gene are
            associated with agenesis of the corpus callosum with peripheral
            neuropathy. [provided by RefSeq, Jul 2008].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC033894.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000354181.8/ ENSP00000346112.3
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-29                BX648195.1         6-34
            30-1066             BC033894.1         6-1042
            1067-1072           AF477977.1         902-907
            1073-2421           BC033894.1         1049-2397
            2422-2925           AF116242.1         1900-2403
            2926-2958           BC051744.1         2757-2789
            2959-3524           AF116242.1         2437-3002
            3525-3564           BX648195.1         4039-4078
            3565-4597           BC033894.1         3540-4572
            4598-8072           AC079203.9         166062-169536       c
FEATURES             Location/Qualifiers
     source          1..8072
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q14"
     gene            1..8072
                     /gene="SLC12A6"
                     /gene_synonym="ACCPN; CMT2II; KCC3; KCC3A; KCC3B"
                     /note="solute carrier family 12 member 6"
                     /db_xref="GeneID:9990"
                     /db_xref="HGNC:HGNC:10914"
                     /db_xref="MIM:604878"
     exon            1..450
                     /gene="SLC12A6"
                     /gene_synonym="ACCPN; CMT2II; KCC3; KCC3A; KCC3B"
                     /inference="alignment:Splign:2.1.0"
     exon            451..793
                     /gene="SLC12A6"
                     /gene_synonym="ACCPN; CMT2II; KCC3; KCC3A; KCC3B"
                     /inference="alignment:Splign:2.1.0"
     CDS             523..3975
                     /gene="SLC12A6"
                     /gene_synonym="ACCPN; CMT2II; KCC3; KCC3A; KCC3B"
                     /note="isoform a is encoded by transcript variant 7;
                     potassium chloride cotransporter 3; potassium chloride
                     cotransporter KCC3a-S3; potassium-chloride transporter-3a;
                     potassium-chloride transporter-3b; K-Cl cotransporter 3;
                     electroneutral potassium-chloride cotransporter 3; solute
                     carrier family 12 (potassium/chloride transporters),
                     member 6; solute carrier family 12 (potassium/chloride
                     transporter), member 6"
                     /codon_start=1
                     /product="solute carrier family 12 member 6 isoform a"
                     /protein_id="NP_001352017.1"
                     /db_xref="CCDS:CCDS58352.1"
                     /db_xref="GeneID:9990"
                     /db_xref="HGNC:HGNC:10914"
                     /db_xref="MIM:604878"
                     /translation="MHPPETTTKMASVRFMVTPTKIDDIPGLSDTSPDLSSRSSSRVR
                     FSSRESVPETSRSEPMSEMSGATTSLATVALDPPSDRTSHPQDVIEDLSQNSITGEHS
                     QLLDDGHKKARNAYLNNSNYEEGDEYFDKNLALFEEEMDTRPKVSSLLNRMANYTNLT
                     QGAKEHEEAENITEGKKKPTKTPQMGTFMGVYLPCLQNIFGVILFLRLTWVVGTAGVL
                     QAFAIVLICCCCTMLTAISMSAIATNGVVPAGGSYFMISRALGPEFGGAVGLCFYLGT
                     TFAAAMYILGAIEIFLVYIVPRAAIFHSDDALKESAAMLNNMRVYGTAFLVLMVLVVF
                     IGVRYVNKFASLFLACVIVSILAIYAGAIKSSFAPPHFPVCMLGNRTLSSRHIDVCSK
                     TKEINNMTVPSKLWGFFCNSSQFFNATCDEYFVHNNVTSIQGIPGLASGIITENLWSN
                     YLPKGEIIEKPSAKSSDVLGSLNHEYVLVDITTSFTLLVGIFFPSVTGIMAGSNRSGD
                     LKDAQKSIPIGTILAILTTSFVYLSNVVLFGACIEGVVLRDKFGDAVKGNLVVGTLSW
                     PSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHSKANGEPTWALL
                     LTAAIAELGILIASLDLVAPILSMFFLMCYLFVNLACALQTLLRTPNWRPRFRYYHWA
                     LSFMGMSICLALMFISSWYYAIVAMVIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARF
                     ALLRLEEGPPHTKNWRPQLLVLLKLDEDLHVKHPRLLTFASQLKAGKGLTIVGSVIVG
                     NFLENYGEALAAEQTIKHLMEAEKVKGFCQLVVAAKLREGISHLIQSCGLGGMKHNTV
                     VMGWPNGWRQSEDARAWKTFIGTVRVTTAAHLALLVAKNISFFPSNVEQFSEGNIDVW
                     WIVHDGGMLMLLPFLLKQHKVWRKCSIRIFTVAQLEDNSIQMKKDLATFLYHLRIEAE
                     VEVVEMHDSDISAYTYERTLMMEQRSQMLRHMRLSKTERDREAQLVKDRNSMLRLTSI
                     GSDEDEETETYQEKVHMTWTKDKYMASRGQKAKSMEGFQDLLNMRPDQSNVRRMHTAV
                     KLNEVIVNKSHEAKLVLLNMPGPPRNPEGDENYMEFLEVLTEGLERVLLVRGGGSEVI
                     TIYS"
     exon            794..838
                     /gene="SLC12A6"
                     /gene_synonym="ACCPN; CMT2II; KCC3; KCC3A; KCC3B"
                     /inference="alignment:Splign:2.1.0"
     exon            839..933
                     /gene="SLC12A6"
                     /gene_synonym="ACCPN; CMT2II; KCC3; KCC3A; KCC3B"
                     /inference="alignment:Splign:2.1.0"
     exon            934..1065
                     /gene="SLC12A6"
                     /gene_synonym="ACCPN; CMT2II; KCC3; KCC3A; KCC3B"
                     /inference="alignment:Splign:2.1.0"
     exon            1066..1212
                     /gene="SLC12A6"
                     /gene_synonym="ACCPN; CMT2II; KCC3; KCC3A; KCC3B"
                     /inference="alignment:Splign:2.1.0"
     exon            1213..1267
                     /gene="SLC12A6"
                     /gene_synonym="ACCPN; CMT2II; KCC3; KCC3A; KCC3B"
                     /inference="alignment:Splign:2.1.0"
     exon            1268..1398
                     /gene="SLC12A6"
                     /gene_synonym="ACCPN; CMT2II; KCC3; KCC3A; KCC3B"
                     /inference="alignment:Splign:2.1.0"
     exon            1399..1640
                     /gene="SLC12A6"
                     /gene_synonym="ACCPN; CMT2II; KCC3; KCC3A; KCC3B"
                     /inference="alignment:Splign:2.1.0"
     exon            1641..1855
                     /gene="SLC12A6"
                     /gene_synonym="ACCPN; CMT2II; KCC3; KCC3A; KCC3B"
                     /inference="alignment:Splign:2.1.0"
     exon            1856..2014
                     /gene="SLC12A6"
                     /gene_synonym="ACCPN; CMT2II; KCC3; KCC3A; KCC3B"
                     /inference="alignment:Splign:2.1.0"
     exon            2015..2113
                     /gene="SLC12A6"
                     /gene_synonym="ACCPN; CMT2II; KCC3; KCC3A; KCC3B"
                     /inference="alignment:Splign:2.1.0"
     exon            2114..2171
                     /gene="SLC12A6"
                     /gene_synonym="ACCPN; CMT2II; KCC3; KCC3A; KCC3B"
                     /inference="alignment:Splign:2.1.0"
     exon            2172..2346
                     /gene="SLC12A6"
                     /gene_synonym="ACCPN; CMT2II; KCC3; KCC3A; KCC3B"
                     /inference="alignment:Splign:2.1.0"
     exon            2347..2465
                     /gene="SLC12A6"
                     /gene_synonym="ACCPN; CMT2II; KCC3; KCC3A; KCC3B"
                     /inference="alignment:Splign:2.1.0"
     exon            2466..2564
                     /gene="SLC12A6"
                     /gene_synonym="ACCPN; CMT2II; KCC3; KCC3A; KCC3B"
                     /inference="alignment:Splign:2.1.0"
     exon            2565..2684
                     /gene="SLC12A6"
                     /gene_synonym="ACCPN; CMT2II; KCC3; KCC3A; KCC3B"
                     /inference="alignment:Splign:2.1.0"
     exon            2685..2789
                     /gene="SLC12A6"
                     /gene_synonym="ACCPN; CMT2II; KCC3; KCC3A; KCC3B"
                     /inference="alignment:Splign:2.1.0"
     exon            2790..2958
                     /gene="SLC12A6"
                     /gene_synonym="ACCPN; CMT2II; KCC3; KCC3A; KCC3B"
                     /inference="alignment:Splign:2.1.0"
     exon            2959..3154
                     /gene="SLC12A6"
                     /gene_synonym="ACCPN; CMT2II; KCC3; KCC3A; KCC3B"
                     /inference="alignment:Splign:2.1.0"
     exon            3155..3324
                     /gene="SLC12A6"
                     /gene_synonym="ACCPN; CMT2II; KCC3; KCC3A; KCC3B"
                     /inference="alignment:Splign:2.1.0"
     exon            3325..3456
                     /gene="SLC12A6"
                     /gene_synonym="ACCPN; CMT2II; KCC3; KCC3A; KCC3B"
                     /inference="alignment:Splign:2.1.0"
     exon            3457..3564
                     /gene="SLC12A6"
                     /gene_synonym="ACCPN; CMT2II; KCC3; KCC3A; KCC3B"
                     /inference="alignment:Splign:2.1.0"
     exon            3565..3749
                     /gene="SLC12A6"
                     /gene_synonym="ACCPN; CMT2II; KCC3; KCC3A; KCC3B"
                     /inference="alignment:Splign:2.1.0"
     exon            3750..3883
                     /gene="SLC12A6"
                     /gene_synonym="ACCPN; CMT2II; KCC3; KCC3A; KCC3B"
                     /inference="alignment:Splign:2.1.0"
     exon            3884..8072
                     /gene="SLC12A6"
                     /gene_synonym="ACCPN; CMT2II; KCC3; KCC3A; KCC3B"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      4597
                     /gene="SLC12A6"
                     /gene_synonym="ACCPN; CMT2II; KCC3; KCC3A; KCC3B"
     regulatory      7836..7841
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC12A6"
                     /gene_synonym="ACCPN; CMT2II; KCC3; KCC3A; KCC3B"
                     /note="hexamer: AATAAA"
     polyA_site      7860
                     /gene="SLC12A6"
                     /gene_synonym="ACCPN; CMT2II; KCC3; KCC3A; KCC3B"
                     /note="major polyA site"
ORIGIN      
        1 agttaccggc tagtaaacaa cagctgcgcg cgcacccgca tcagctggcg ccgggatact
       61 gcacctgcga cctccttctc cctcaccctg ctgcccagtc aggccagcct gcgtggccgt
      121 ctcctcctct actgggaccc tgaggaagaa cgacgagggg gtgacctgcg ccggggcggg
      181 ggtaggggag aacgcgcctc cggcaaaggg ggtggctcgt cctgtcacac gcaggagaaa
      241 cctagcgagc ggaccgtggc tcagtggact agctacgact tgccacgact ctgagctgca
      301 gtccgaggtg tcttgatccg ctaacctcct cccagacccc gaggaccttt ctgctaggag
      361 aagagcgggc gcgccacccc caccgacctc tcggagacag gaatccgctc tgcccctgca
      421 tcctcctctg ctcacccttc tctcagtagt tcaaaagtag ctagcgtaag aggaagattt
      481 tgcgaggttc cccccacttt ttttgttctt aaaaagaaca aaatgcatcc tccagaaacc
      541 accaccaaga tggcttcagt tcggttcatg gtgacaccga caaagatcga tgacattcca
      601 ggtttgtcag acaccagtcc ggacctcagc tctcgatcta gttcccgagt aagatttagc
      661 tcccgggaaa gcgtgcctga aacaagccgg agtgagccta tgagtgagat gtctggggcc
      721 accacttcgc tggcaactgt tgcactggat ccacccagtg accggacttc tcacccccag
      781 gatgtcatcg aggacctgag tcagaactcc atcacagggg aacacagcca actgttagac
      841 gacggacata agaaagctcg aaatgcttat ctcaataatt ccaattatga agaaggagat
      901 gaatattttg ataaaaattt ggcactcttt gaggaagaaa tggacaccag accgaaggtg
      961 tcttccctcc tcaaccgcat ggccaattac actaatctga ctcaaggagc aaaggaacat
     1021 gaagaggcag aaaacatcac tgaagggaaa aagaagccca ccaagacccc ccaaatgggt
     1081 accttcatgg gtgtctacct cccatgtcta caaaatattt ttggagtgat ccttttttta
     1141 cgccttacat gggtggtggg cacagctgga gttcttcagg cttttgcaat tgtccttatc
     1201 tgctgctgct gtacaatgtt gactgctatc tccatgagtg ccattgccac taatggagtg
     1261 gtgccagctg ggggctcata ctttatgatt tcccgggcac tgggcccaga gtttggtggg
     1321 gctgttggcc tctgctttta tcttggtacc acatttgcag cagccatgta catccttggt
     1381 gccattgaaa tctttctggt ctatatcgtc ccccgagctg ccatctttca cagtgatgac
     1441 gcactcaagg aatcagcagc catgctaaat aacatgcgtg tctacggcac agctttcttg
     1501 gtccttatgg tattagtggt atttatcggc gtacgctatg tgaacaagtt tgcctcactt
     1561 ttcctggcct gtgtcattgt gtccatcttg gccatctatg ctggagccat caagtcttct
     1621 tttgctcctc cacacttccc ggtctgcatg ctgggtaacc gcaccctttc atcaagacac
     1681 attgacgttt gctctaagac caaggaaatt aacaacatga cagtcccatc aaagttatgg
     1741 ggattcttct gtaactcgag tcaatttttc aatgccacct gtgatgaata ctttgttcac
     1801 aataacgtca cttcaatcca gggcattcct ggattggcta gtggtataat tacagagaat
     1861 ctttggagta attacctacc caagggagag atcatcgaaa agccttcagc caaatcttct
     1921 gatgtcttag gcagcttaaa ccatgaatat gttcttgttg acatcaccac ctccttcacg
     1981 cttctggtgg gaatcttctt tccctctgtt acaggtatca tggctggatc aaacagatct
     2041 ggagatctga aagatgctca gaagtctatt ccgattggta ctatccttgc catcctgacc
     2101 acctcctttg tttatttaag caatgttgtc ctttttggtg catgtattga aggggttgtt
     2161 ctcagagaca agttcggtga tgctgtgaaa ggtaatttgg tggtaggcac cttatcttgg
     2221 ccatccccat gggtgattgt tattggctcc ttcttttcaa catgtggggc tggacttcag
     2281 agcctcacag gtgcaccgag gctgctacaa gctattgcca aggataacat cataccgttt
     2341 ctgagggttt ttggccacag caaagccaat ggggaaccta cctgggcttt acttctaact
     2401 gctgccattg cagagcttgg aatactcatt gcctccctgg atcttgtggc cccaattctt
     2461 tccatgtttt ttctcatgtg ttacctcttt gtaaacttgg catgtgcctt gcaaacatta
     2521 cttcgaacac ccaactggag accccgattc cgctactacc attgggccct ttctttcatg
     2581 ggaatgagta tctgtctggc tctgatgttc atttcttcct ggtattatgc cattgtagcc
     2641 atggtaatag ctggtatgat ctacaagtac attgaatacc aaggagctga gaaagaatgg
     2701 ggtgatggta tccgtgggct gtccctcagt gcagcccggt ttgctttgct tcgattggag
     2761 gaaggacctc cacacactaa aaactggagg cctcagttgc ttgtattact gaaactagat
     2821 gaagacttac atgtcaagca tcctcgcctc ctcacctttg cctcacagct caaagcagga
     2881 aaaggtctca ctattgtggg ctctgtcatc gtggggaact tcctagagaa ctacggtgaa
     2941 gctttagctg ctgagcagac cataaagcac ctaatggagg cagagaaggt aaaaggattc
     3001 tgccagctgg tggtggccgc caagctgaga gagggcattt cccacctcat ccagtcatgt
     3061 ggccttgggg gcatgaagca caacacggtg gtgatgggct ggcctaatgg ctggcgtcaa
     3121 agcgaagatg cccgcgcttg gaagactttt attggcacag ttcgagtgac aactgctgcc
     3181 catcttgcac tgctggtggc taaaaacatc tccttctttc ccagcaatgt ggagcaattt
     3241 tctgagggca acattgatgt gtggtggatt gtgcatgatg gggggatgct tatgctacta
     3301 ccattcctac tgaaacagca caaggtgtgg cgaaagtgca gcatacggat cttcacagta
     3361 gcccaattag aagacaacag tatccaaatg aagaaggacc tagccacctt cctatatcac
     3421 ttacgcattg aggcggaggt agaagtggtg gagatgcatg acagtgatat atcagcatat
     3481 acttacgagc gcactttgat gatggaacaa aggtcccaga tgctccggca catgcggcta
     3541 tccaaaacag agcgagacag agaggcacaa ttggtgaaag accgaaactc aatgctacga
     3601 ttgaccagca ttggctctga tgaggacgaa gagacagaaa cctatcagga gaaggtgcac
     3661 atgacttgga caaaagacaa gtacatggca tcccggggac aaaaagcgaa gtcaatggaa
     3721 ggattccagg acctgcttaa catgcgtccg gaccagtcca atgtgaggcg gatgcataca
     3781 gcagtgaaac tcaacgaggt tatagttaac aagtcccatg aagcaaagct ggttttattg
     3841 aatatgccag ggccaccccg aaaccctgag ggtgatgaaa actacatgga gttcctagag
     3901 gtgcttaccg agggactaga gcgagtccta cttgtccggg gtggtggcag tgaagtgatc
     3961 accatttatt cataacctac tctgaatgac cgtgcttgac ctgttttctt aaaaggccta
     4021 cgtcctccat ggaagtgcca gctcattact accactccca ctcaactaga agcctgtgtt
     4081 ctgtacacat catactgaac tcttgatgag ctgagcctca agtacctgtg taaaagagct
     4141 cccatctgat ctgcagtcat tacagaaaaa gcaaatattc cctcaacatc agaacaatgc
     4201 tcaagtcttt caagccacgt ctgagcagtc aaaggcaaat tagaattaac aagctgagcc
     4261 aataaatgaa ttggtaaaag ggatgctaga aattcaactg aagaaaaaaa gcaagtcaag
     4321 tacgtattca gcattaaaga tgaatctcag aagtcatggt tcaatgttga cactgtgagg
     4381 ataacaacta gagacagctt catcttacta aagaatttat ggtcaagtat atttggacct
     4441 attatcctcg gcaagccaag atgcaaacat tttttagcta tatttcttta gtatacccac
     4501 tgctgtaatt ttatattagg atactaactt gaaacatggc tgcagcctct acttcttcaa
     4561 aaacatcccc ccaaaatacc agatttaaat atccaaatac tgtgtcagtg tatacttcaa
     4621 ctgttttgtt tctcttaact caaaacccat tctgtcattg taacacaggc agtgttacca
     4681 tggaaattgt ttttggtaag gtaaaaatta tttctttcag gttcctttca aatggccgaa
     4741 ctaagagaac tccaaaagga agctattaac atttactgaa atagtctgtg gttctctata
     4801 caacatggac aaaacataca agctgttgtc taagaaatgc aggtcatggt gcacattaat
     4861 caacaggttc ttctcccccc gcccccaccc ccgcgccttt ttttatgccc tcttattaag
     4921 cacaggtcaa aaaatcttaa ggaacaaata ctaggaaaac tggctaaaca tccttccctg
     4981 aaagcactgg accatctttt aaagggcaca tctagcaatg gaattggaag ttctagagcc
     5041 acactctttc tttagtgcca acgttactga gcggctactc tccttaccta ctttactaaa
     5101 atggcataca gattggcatt gacctgttga agaaatttta ccactaagaa atcttttgcc
     5161 tcatctacaa agccatagtt aagacttgag gcattcaact taatgccaag tataagcatt
     5221 tccccctttt tctttcaatt ttattcttca ttctgactac agaaacttgg ccctgaacta
     5281 cgagattagc caagccaaca gtcttctaat ttgttttcag aaacttcagg ccgggtgccg
     5341 tggctcacac ctgtaatccc agcactttgg gaggccaagg caggtgaatc acttgaggtc
     5401 aggagttcga gaccatcctg gccaacatgg tgaaaacctg tctatactaa aaatacaaaa
     5461 attagctggg catggtggtg catgcctgtg atcccagcaa cacggaaggc taaggcagga
     5521 ggatcatttg aacccaggag gcggaggttg cagtgagcca ggattgcacc tctgcactcc
     5581 agcctgggta acagagtgat atgtttcaaa aagaaagttc agtgtgccaa ggaaatactt
     5641 gtgtaagctc agctttttat ttactaatat actgatcaaa catctggctt acaagcatag
     5701 ggaaactact tatgtggctg gctgagcagt aatttcaagt atgtgaaatc ttcatttctt
     5761 ttacagaata gttatttaga gaaacttttc ttgcctgtat tcatggctgt ttttgaattt
     5821 caatttatta gtcataaata gtgccactct tctttgatta ctaaatagga catggagcaa
     5881 gctgacctag gtgtcacaaa ttattcattt gacctgtaag gacagagaaa acccttcaat
     5941 atagcttaag aaattgagta attttgggcc gggtgcagtg actcacgcct gtaatcccag
     6001 cactttggga ggccgaggca ggcggatcac gaggtcagga gatttgagac catcctggct
     6061 aacacggtga aaccctgtct ctacaaaaat acaaaaaaat tagtcgggca tggtggcggg
     6121 cgcctgtagt cccagctact tgggaggctg aggcgggaga atggcgtgaa cccgggaggt
     6181 ggaacttgca gtgaaccgag attgcaccac tgcactccag cctgggcgac agagggagac
     6241 tccatctcaa aaaaaaaaaa aaagaaagaa agaaagaaat tgagtaattt tgatttgttc
     6301 cctttgcttg cttcaacctc caggcccact gaggatggca aagcggatag gatcttaagt
     6361 ctttttttct gttacttcct acagatggta gaagcattat ttacaactcc cactgcaaca
     6421 ctccattgct tagttgagtt taacagtagt gagtcatgtc ctgcaggatc cagtatcttt
     6481 tttttttttt tccgagacgt agtttcgctc ttgttgccca ggctggagtg aaatggcaca
     6541 atcttggctc accgcaacct ctgcctcctg ggttcaagcg attctcctgc ctcagcctcc
     6601 cgagtagctg gaattacaag catgtgccac cacacccagc taattttgta ttttcagtag
     6661 agacggggtt tctctactga aactgatgtt ggtcaggctg gtctcgaact cccaacctca
     6721 ggtgatccgc ccgccttggc ctcccaaagt gctgggatta caggcgtgag ccactgtgcc
     6781 caaccaggat ccagtatctt aaaggcccat actgtaaagg tcagcctgcc taggaattct
     6841 gccacattta cacacacccc tggccaaagt aatatttttt ttttccatgt agaacaaatt
     6901 atgggcagaa agtaaagtta gttttagaag tgctgtttgt ggttggagaa ttgtaaaaat
     6961 cttttaaagg gataagggaa ttagtggctt ttaatataac cactgcttca gcccgaaaca
     7021 caaaatcact gttacatgga aaactggtta ttaggccaga tttatattca tgtctgtcta
     7081 gagtatttca tgtgtgtatg tgttgcttac gttgtctgtc tccaaagtct ctcatctctt
     7141 ttaatcactg caaataaagc aatactgaaa acttgagaga atgcacctta ggggaagggg
     7201 ctatttcaag acagaaaaca aaggaaatac ctgccttttg aataagatcc cgcttttgag
     7261 tcttacctat gactttacca gggtagatta gaaatacaca tcctcctgct gaccctctgc
     7321 cttcaatagc tatctatctt aaaagctgaa gtttgaactg cagcatctgc ttgacaggtg
     7381 ccaggttcct tcggcagggg gacgatcact ctatatatct tccgttgcct cagattcctg
     7441 tggcccaagg atcccaccaa tcctcgttcc ccctaaacat gctaacaaaa atcccttatc
     7501 gtgggtatta aaataataac agttacactg tatgcatatt tatgtgctcc ttttgtctgg
     7561 tttttctttt catcatgtat aagctgaatt cagcattagt ttctcacatc ttcccccagg
     7621 tatccccaac agaattttta tgtcccagct tgtattaaat agaagtgaaa tattaaggaa
     7681 aataaggaac ttgtgcaact ttttttatgc attgttctca accatttaat ttattgaaag
     7741 gagatgctgc aacagttctt gatttagcag cagttattct cttgtttaca tagttatgtt
     7801 tttttgttgt tgttttgctc tgtactggaa acaaaaataa agttttctac attattttca
     7861 gccttgggtt atggtatagt ttctttgtgt ttgtcgtaat atgcacattg tccttctagg
     7921 acctgtcacc ccaccatgga gaaaagagtc ttttggttct ttttaacata agtgattagt
     7981 ttaagagtat gctgaggagc cactgggctt aaagaaggat gtaaataaga cccaaataca
     8041 tagggaccag gcgctgcttt ctcatgttca ca
//
