LOCUS       NM_020708               6026 bp    mRNA    linear   PRI 11-OCT-2024
DEFINITION  Homo sapiens solute carrier family 12 member 5 (SLC12A5),
            transcript variant 2, mRNA.
ACCESSION   NM_020708
VERSION     NM_020708.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6026)
  AUTHORS   Pearson,A.C. and Ostroumov,A.
  TITLE     Midbrain KCC2 downregulation: Implications for stress-related and
            substance use behaviors
  JOURNAL   Curr Opin Neurobiol 88, 102901 (2024)
   PUBMED   39142020
  REMARK    GeneRIF: Midbrain KCC2 downregulation: Implications for
            stress-related and substance use behaviors.
            Review article
REFERENCE   2  (bases 1 to 6026)
  AUTHORS   Kok,M., Hartnett-Scott,K., Happe,C.L., MacDonald,M.L., Aizenman,E.
            and Brodsky,J.L.
  TITLE     The expression system influences stability, maturation efficiency,
            and oligomeric properties of the potassium-chloride co-transporter
            KCC2
  JOURNAL   Neurochem Int 174, 105695 (2024)
   PUBMED   38373478
  REMARK    GeneRIF: The expression system influences stability, maturation
            efficiency, and oligomeric properties of the potassium-chloride
            co-transporter KCC2.
REFERENCE   3  (bases 1 to 6026)
  AUTHORS   Chen,J., Wang,H., Deng,C. and Fei,M.
  TITLE     SLC12A5 as a novel potential biomarker of glioblastoma multiforme
  JOURNAL   Mol Biol Rep 50 (5), 4285-4299 (2023)
   PUBMED   36917367
  REMARK    GeneRIF: SLC12A5 as a novel potential biomarker of glioblastoma
            multiforme.
REFERENCE   4  (bases 1 to 6026)
  AUTHORS   Tong,Q., Qin,W., Li,Z.H., Liu,C., Wang,Z.C., Chu,Y. and Xu,X.D.
  TITLE     SLC12A5 promotes hepatocellular carcinoma growth and ferroptosis
            resistance by inducing ER stress and cystine transport changes
  JOURNAL   Cancer Med 12 (7), 8526-8541 (2023)
   PUBMED   36645171
  REMARK    GeneRIF: SLC12A5 promotes hepatocellular carcinoma growth and
            ferroptosis resistance by inducing ER stress and cystine transport
            changes.
REFERENCE   5  (bases 1 to 6026)
  AUTHORS   Li,Y., Li,Y.L., Liu,Y.L., Fu,J., Zhang,W.W. and Piao,Y.S.
  TITLE     [Expression of cation chloride cotransporter (NKCC1/KCC2) in brain
            tissue of children with focal cortical dysplasia type ]
  JOURNAL   Zhonghua Bing Li Xue Za Zhi 51 (11), 1123-1128 (2022)
   PUBMED   36323541
  REMARK    GeneRIF: [Expression of cation chloride cotransporter (NKCC1/KCC2)
            in brain tissue of children with focal cortical dysplasia type ].
REFERENCE   6  (bases 1 to 6026)
  AUTHORS   Hebert,S.C., Mount,D.B. and Gamba,G.
  TITLE     Molecular physiology of cation-coupled Cl- cotransport: the SLC12
            family
  JOURNAL   Pflugers Arch 447 (5), 580-593 (2004)
   PUBMED   12739168
  REMARK    Review article
REFERENCE   7  (bases 1 to 6026)
  AUTHORS   Brauer,M., Frei,E., Claes,L., Grissmer,S. and Jager,H.
  TITLE     Influence of K-Cl cotransporter activity on activation of
            volume-sensitive Cl- channels in human osteoblasts
  JOURNAL   Am J Physiol Cell Physiol 285 (1), C22-C30 (2003)
   PUBMED   12637262
  REMARK    GeneRIF: human osteoblasts express functional K-Cl cotransporters
            in their cell membrane that seem to be able to induce the indirect
            activation of volume-sensitive Cl- channels by KCl through an
            increase in the intracellular ions, water influx and cell swelling.
REFERENCE   8  (bases 1 to 6026)
  AUTHORS   Song,L., Mercado,A., Vazquez,N., Xie,Q., Desai,R., George,A.L. Jr.,
            Gamba,G. and Mount,D.B.
  TITLE     Molecular, functional, and genomic characterization of human KCC2,
            the neuronal K-Cl cotransporter
  JOURNAL   Brain Res Mol Brain Res 103 (1-2), 91-105 (2002)
   PUBMED   12106695
  REMARK    GeneRIF: Expression of hKCC2 cRNA in Xenopus laevis oocytes results
            in significant Cl(-)-dependent (86)Rb(+) uptake under isotonic
            conditions
REFERENCE   9  (bases 1 to 6026)
  AUTHORS   Sallinen,R., Tornberg,J., Putkiranta,M., Horelli-Kuitunen,N.,
            Airaksinen,M.S. and Wessman,M.
  TITLE     Chromosomal localization of SLC12A5/Slc12a5, the human and mouse
            genes for the neuron-specific K(+)-Cl(-) cotransporter (KCC2)
            defines a new region of conserved homology
  JOURNAL   Cytogenet Cell Genet 94 (1-2), 67-70 (2001)
   PUBMED   11701957
  REMARK    GeneRIF: chromosome 20 mapping
            Erratum:[Cytogenet Cell Genet. 2002;98(2-3):232]
REFERENCE   10 (bases 1 to 6026)
  AUTHORS   McTague,A. and Kurian,M.A.
  TITLE     SLC12A5-Related Epilepsy of Infancy with Migrating Focal Seizures
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   30763027
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL162458.10.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_020708.4.
            
            Summary: K-Cl cotransporters are proteins that lower intracellular
            chloride concentrations below the electrochemical equilibrium
            potential. The protein encoded by this gene is an integral membrane
            K-Cl cotransporter that can function in either a net efflux or
            influx pathway, depending on the chemical concentration gradients
            of potassium and chloride. The encoded protein can act as a
            homomultimer, or as a heteromultimer with other K-Cl
            cotransporters, to maintain chloride homeostasis in neurons.
            Alternative splicing results in two transcript variants encoding
            different isoforms. [provided by RefSeq, Sep 2008].
            
            Transcript Variant: This variant (2) differs in the 5' UTR and 5'
            coding region, compared to variant 1. The resulting isoform (2,
            also known as KCC2b) has a distinct N-terminus and is shorter than
            isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC132670.1, AF208159.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2145743, SAMEA2154665
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000243964.7/ ENSP00000243964.4
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-182               AL162458.10        115631-115812
            183-277             AL162458.10        121364-121458
            278-409             AL162458.10        121820-121951
            410-556             AL162458.10        122193-122339
            557-611             AL162458.10        123157-123211
            612-742             AL162458.10        123671-123801
            743-984             AL162458.10        126789-127030
            985-1196            AL162458.10        127745-127956
            1197-1367           AL162458.10        129569-129739
            1368-1466           AL162458.10        130049-130147
            1467-1524           AL162458.10        130292-130349
            1525-1699           AL162458.10        131382-131556
            1700-1818           AL162458.10        132294-132412
            1819-1917           AL162458.10        132754-132852
            1918-2037           AL162458.10        133870-133989
            2038-2142           AL162458.10        134397-134501
            2143-2311           AL162458.10        136038-136206
            2312-2507           AL162458.10        138091-138286
            2508-2677           AL162458.10        139373-139542
            2678-2809           AL162458.10        139994-140125
            2810-2917           AL162458.10        141332-141439
            2918-3040           AL162458.10        142566-142688
            3041-3240           AL162458.10        142781-142980
            3241-3255           AL162458.10        143313-143327
            3256-3389           AL162458.10        143586-143719
            3390-6026           AL162458.10        143930-146566
FEATURES             Location/Qualifiers
     source          1..6026
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q13.12"
     gene            1..6026
                     /gene="SLC12A5"
                     /gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
                     /note="solute carrier family 12 member 5"
                     /db_xref="GeneID:57468"
                     /db_xref="HGNC:HGNC:13818"
                     /db_xref="MIM:606726"
     exon            1..182
                     /gene="SLC12A5"
                     /gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
                     /inference="alignment:Splign:2.1.0"
     CDS             131..3481
                     /gene="SLC12A5"
                     /gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
                     /note="isoform 2 is encoded by transcript variant 2;
                     erythroid K-Cl cotransporter 2; neuronal K-Cl
                     cotransporter; solute carrier family 12
                     (potassium/chloride transporter), member 5; electroneutral
                     potassium-chloride cotransporter 2"
                     /codon_start=1
                     /product="solute carrier family 12 member 5 isoform 2"
                     /protein_id="NP_065759.1"
                     /db_xref="CCDS:CCDS13391.1"
                     /db_xref="GeneID:57468"
                     /db_xref="HGNC:HGNC:13818"
                     /db_xref="MIM:606726"
                     /translation="MLNNLTDCEDGDGGANPGDGNPKESSPFINSTDTEKGKEYDGKN
                     MALFEEEMDTSPMVSSLLSGLANYTNLPQGSREHEEAENNEGGKKKPVQAPRMGTFMG
                     VYLPCLQNIFGVILFLRLTWVVGIAGIMESFCMVFICCSCTMLTAISMSAIATNGVVP
                     AGGSYYMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGTIEILLAYLFPAMAIFKAED
                     ASGEAAAMLNNMRVYGTCVLTCMATVVFVGVKYVNKFALVFLGCVILSILAIYAGVIK
                     SAFDPPNFPICLLGNRTLSRHGFDVCAKLAWEGNETVTTRLWGLFCSSRFLNATCDEY
                     FTRNNVTEIQGIPGAASGLIKENLWSSYLTKGVIVERSGMTSVGLADGTPIDMDHPYV
                     FSDMTSYFTLLVGIYFPSVTGIMAGSNRSGDLRDAQKSIPTGTILAIATTSAVYISSV
                     VLFGACIEGVVLRDKFGEAVNGNLVVGTLAWPSPWVIVIGSFFSTCGAGLQSLTGAPR
                     LLQAISRDGIVPFLQVFGHGKANGEPTWALLLTACICEIGILIASLDEVAPILSMFFL
                     MCYMFVNLACAVQTLLRTPNWRPRFRYYHWTLSFLGMSLCLALMFICSWYYALVAMLI
                     AGLIYKYIEYRGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLVRVDQ
                     DQNVVHPQLLSLTSQLKAGKGLTIVGSVLEGTFLENHPQAQRAEESIRRLMEAEKVKG
                     FCQVVISSNLRDGVSHLIQSGGLGGLQHNTVLVGWPRNWRQKEDHQTWRNFIELVRET
                     TAGHLALLVTKNVSMFPGNPERFSEGSIDVWWIVHDGGMLMLLPFLLRHHKVWRKCKM
                     RIFTVAQMDDNSIQMKKDLTTFLYHLRITAEVEVVEMHESDISAYTYEKTLVMEQRSQ
                     ILKQMHLTKNEREREIQSITDESRGSIRRKNPANTRLRLNVPEETAGDSEEKPEEEVQ
                     LIHDQSAPSCPSSSPSPGEEPEGEGETDPEKVHLTWTKDKSVAEKNKGPSPVSSEGIK
                     DFFSMKPEWENLNQSNVRRMHTAVRLNEVIVKKSRDAKLVLLNMPGPPRNRNGDENYM
                     EFLEVLTEHLDRVMLVRGGGREVITIYS"
     exon            183..277
                     /gene="SLC12A5"
                     /gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
                     /inference="alignment:Splign:2.1.0"
     exon            278..409
                     /gene="SLC12A5"
                     /gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
                     /inference="alignment:Splign:2.1.0"
     exon            410..556
                     /gene="SLC12A5"
                     /gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
                     /inference="alignment:Splign:2.1.0"
     exon            557..611
                     /gene="SLC12A5"
                     /gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
                     /inference="alignment:Splign:2.1.0"
     exon            612..742
                     /gene="SLC12A5"
                     /gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
                     /inference="alignment:Splign:2.1.0"
     exon            743..984
                     /gene="SLC12A5"
                     /gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
                     /inference="alignment:Splign:2.1.0"
     exon            985..1196
                     /gene="SLC12A5"
                     /gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1197..1367
                     /gene="SLC12A5"
                     /gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1368..1466
                     /gene="SLC12A5"
                     /gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1467..1524
                     /gene="SLC12A5"
                     /gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1525..1699
                     /gene="SLC12A5"
                     /gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1700..1818
                     /gene="SLC12A5"
                     /gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1819..1917
                     /gene="SLC12A5"
                     /gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1918..2037
                     /gene="SLC12A5"
                     /gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2038..2142
                     /gene="SLC12A5"
                     /gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2143..2311
                     /gene="SLC12A5"
                     /gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2312..2507
                     /gene="SLC12A5"
                     /gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2508..2677
                     /gene="SLC12A5"
                     /gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2678..2809
                     /gene="SLC12A5"
                     /gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2810..2917
                     /gene="SLC12A5"
                     /gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2918..3040
                     /gene="SLC12A5"
                     /gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
                     /inference="alignment:Splign:2.1.0"
     exon            3041..3240
                     /gene="SLC12A5"
                     /gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
                     /inference="alignment:Splign:2.1.0"
     exon            3241..3255
                     /gene="SLC12A5"
                     /gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
                     /inference="alignment:Splign:2.1.0"
     exon            3256..3389
                     /gene="SLC12A5"
                     /gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
                     /inference="alignment:Splign:2.1.0"
     exon            3390..6026
                     /gene="SLC12A5"
                     /gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6001..6006
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC12A5"
                     /gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
                     /note="hexamer: AATAAA"
     polyA_site      6026
                     /gene="SLC12A5"
                     /gene_synonym="DEE34; EIEE34; EIG14; hKCC2; KCC2"
                     /note="major polyA site"
ORIGIN      
        1 actgcagctt cttcctccgt ggagcggaga gcgagacaga gctacagcga acgagagagc
       61 ggcgaaggcg ggtagagggg cgcgggcgag gcggcgcagc catccccgga ccaggggccg
      121 cgccgccacc atgctaaaca acctgacgga ctgcgaggac ggcgatgggg gagccaaccc
      181 gggtgatggc aaccccaagg aaagcagtcc cttcatcaac agcaccgaca cagagaaggg
      241 aaaggagtat gatggcaaga acatggcctt gtttgaggag gagatggaca ccagccctat
      301 ggtgtcctcc ttgctcagtg gcctggccaa ctacaccaac ctgccccagg gaagtaggga
      361 gcatgaagag gcagaaaaca atgagggtgg aaaaaagaag ccggtgcagg ccccacgcat
      421 gggcaccttc atgggcgtgt acctgccgtg cctgcagaac atctttggcg tcatcctctt
      481 cctgcggctc acctgggtgg tgggcattgc aggcatcatg gagtccttct gcatggtgtt
      541 catctgctgc tcctgtacga tgctcacggc catctccatg agtgcaattg caacgaatgg
      601 tgttgtgcct gctggtggct cctactacat gatttccagg tctctgggcc cagagtttgg
      661 gggtgccgtg ggcctctgct tctacctggg cactaccttt gcaggagcca tgtacatcct
      721 gggcaccatc gaaatcctgc tggcttacct cttcccagcc atggccatct tcaaggcaga
      781 agatgccagt ggggaggcag cagccatgct gaacaacatg cgtgtttacg gcacctgtgt
      841 gctcacctgc atggccactg tggtgtttgt gggtgtcaag tatgtcaaca agtttgccct
      901 tgtcttcctg ggttgtgtca tcctctccat cctggccatc tatgctgggg tcatcaagtc
      961 tgccttcgac ccacccaact tcccgatctg cctcctgggt aaccgcacgc tgtctcgcca
     1021 tggctttgat gtctgtgcca agctggcttg ggaaggaaat gagacggtga ccacacggct
     1081 atggggcctt ttctgctcct ctcgcttcct caacgccacc tgtgatgaat acttcacccg
     1141 aaacaatgtc acagagatcc agggcatccc tggtgctgcc agtggcctca tcaaagagaa
     1201 cctctggagc tcctacctga ccaagggcgt gattgtggag aggagtggga tgacctcggt
     1261 gggcctggcc gatggcactc ctatcgacat ggaccaccct tatgtcttca gtgatatgac
     1321 ctcctacttc accctgctgg ttggcatcta cttcccctca gtcacaggga tcatggctgg
     1381 ttctaaccgc tctggggacc tgagggatgc ccagaagtca atccccactg gcaccatcct
     1441 ggccatcgcc accacctctg ctgtctacat cagctccgtt gttctgtttg gggcctgcat
     1501 tgagggggtc gtcctgcggg acaagtttgg cgaagctgtg aatggcaacc tcgtggtggg
     1561 cactctggcc tggccatctc catgggtaat tgtcatcgga tccttcttct ccacctgtgg
     1621 ggctgggctg cagagcctca cgggggcccc acgcctgctg caggccatct cgagggatgg
     1681 cattgtgccc ttcctgcagg tctttggcca tggcaaggcc aatggagagc cgacctgggc
     1741 cctgctcctg actgcctgca tctgcgagat tggcatcctc attgcatccc tcgacgaggt
     1801 ggcccccatc ctctctatgt tcttcctgat gtgctacatg tttgtgaatc tggcctgtgc
     1861 agtgcagacg ctgctgagga cacccaactg gaggccacgc tttcgatatt accactggac
     1921 cctctccttc ctgggcatga gcctctgcct ggccctcatg ttcatctgct cctggtatta
     1981 tgcactggta gccatgctca ttgctggact catctacaag tacattgagt accgtggggc
     2041 agagaaggag tggggcgatg ggatacgagg tctgtctctc agtgcggctc gctatgccct
     2101 cttacgcctg gaggaagggc ccccacacac caagaactgg aggccacagc tgctggtgct
     2161 ggtgcgtgtg gaccaagacc agaatgtggt gcacccccag ctgctctcac tgacctccca
     2221 gctgaaggcg gggaagggcc tgaccatcgt gggctctgtc cttgagggca cctttctgga
     2281 aaatcatcca caggcccagc gggcagaaga gtctatcagg cgcctgatgg aggcagagaa
     2341 ggtgaagggc ttctgccagg tggtgatctc ctccaacttg cgtgatggcg tgtcccatct
     2401 gatccagtcc gggggcctcg gggggctgca gcacaacact gtgcttgttg gctggccccg
     2461 caactggcgc cagaaggaag atcatcagac gtggaggaac ttcattgagc tggtccggga
     2521 aaccacagct ggccacttag ccctgctggt caccaagaac gtttccatgt ttcctgggaa
     2581 ccctgagcgc ttctctgagg gcagcatcga cgtttggtgg attgtgcacg atggaggcat
     2641 gctcatgctg ctgcccttcc tgctgcggca ccacaaggtc tggcggaagt gcaagatgcg
     2701 tatcttcact gtggcccaga tggatgacaa tagcatccag atgaagaagg atctgaccac
     2761 atttctgtat catttacgca tcactgcgga ggtcgaggtg gtggagatgc atgagagcga
     2821 catctcagct tacacctatg agaagacgtt ggtgatggag cagcgttccc agatcctcaa
     2881 acagatgcat ttaaccaaga atgagcggga gcgggagatc cagagtatca cagatgagtc
     2941 acgaggctca atccggagaa agaatccagc caacacgcgg ctccgcctga acgtcccaga
     3001 agagacggct ggtgacagtg aagagaagcc agaggaggag gtgcagctga tccacgatca
     3061 gagtgctccc agctgcccca gcagctcccc gtccccaggg gaggagcctg agggggaagg
     3121 ggagacagat ccggagaagg tgcatctcac ctggaccaag gacaagtcgg tggcagagaa
     3181 gaataagggc cccagtcctg tctcctctga gggcatcaag gacttcttca gcatgaagcc
     3241 ggagtgggag aacttgaacc agtccaacgt gcggcgcatg cacacggccg tgcggctgaa
     3301 cgaggtcatc gtgaagaaat cccgggacgc caagcttgtt ttgctcaaca tgcctgggcc
     3361 tccccgcaac cgcaatggtg atgaaaacta catggagttt ctcgaggtcc tcacagagca
     3421 cctggaccgg gtgatgctgg tccgcggcgg cggccgcgag gtcatcacca tctactcctg
     3481 agaaccagga cctgccaccc gggcccgagc gcgcccggcc cgcggctccg gagccctcgc
     3541 cgcgcccccc gccgctgtca ccgtttacat acagaccctg tgcccgtgtc ctggcccctt
     3601 accccgctgc ctgaagcccg gaggccacgc ctgttggggc tgattcggag agggcgcccc
     3661 gccgcgcaga gaccagagct cctcagtgcc agtttggccc ctgggtcttc gctgcccttt
     3721 ttctaagccc ggcctcgtct cgccggagga gacgctgcaa taaaggttgg gagaaggcgc
     3781 ggaaaggaga ggagctgggg ccttggggac ccccaggtag tccatgcggc ccattcctcc
     3841 ccttcccact cccgccgcgg tcctcgctct gcgctcctcc ggcgctgctc cctggctccc
     3901 ggcggcccgg aggcccgcgg ggtgggaagg ccgcgcttgc cgtctccgcc gccccttctc
     3961 gccgagccgt ggggcgcggg cggccgagcc tatacatagt gtacaggaga catcgcgtgt
     4021 atttttaacg tccccatatt tatgtgacta gaagcgcaac agacttctcg ccatagtcga
     4081 gctctcccgc tgggggcact gcggggaggc gaggcctcgg gaagctgaat tttccttgac
     4141 gtccaagagt ttgagagcga aagtgcttta ggcccaggcg ggggtcgtgg cctcgttccc
     4201 tcgacacctc cgtcctgctc tcgcctcttc gccctttccg cgcgcccttg gcttcccacc
     4261 ctcctctcca gtccttttcc gagatgaggt gagacaaggg tccaactttt cctggattcg
     4321 cctcccagcg gacgtgagct tccactgcgg ctgcagagac gcgagcaacc tcttctcatc
     4381 ggctcttatg caagttgggg ccaggatagg ggaggggtgc tcctcaagag gaagaaaccg
     4441 agaggcccgc gccccaccga ggaagccccg ccccggtgcc ttcgctgggg agcaggcgtc
     4501 tctcctcagt cggcttgtcg cctgctcccc gtatcccatg gctcctcgcc aaagactgaa
     4561 attgtggagc tggagggcgc cccctccccg gagtttcctc cctgggacaa gtgagggagg
     4621 agggggccga ttctggttta ggggccggac ccactgagag gccccagagc cgcccgtgat
     4681 gttcctcccc cgtccccatc tggcagctcc tgtctcgcct gagggaccca gccgccttct
     4741 ccgtgctctg gggccgggcc tcgctgctta gcagcggcct ctagctccgt ctcccgggga
     4801 cctgggcctg agggagggct ggagtcgcac gcgctttgtc cttagcgcct gtctgctctc
     4861 ctctaactag gacccagggc ctttggcttc cccagctcat ccttggccct tccgctccac
     4921 cagcctggtc tgaggcgtgc tctgtcctta gagaaggcgc ggtggccggg ttcccttccc
     4981 ctagggcaca ttactaaggg ggtcaggcac tgcatgctcg ttccagcacc atctgggact
     5041 gggtacagta cctccagccc cagggccctg acctgcgcac ctagcttgac atctcacgca
     5101 cctcccagag ctggcgccac tgagtaatcc ggacctcacc acctcttttc ctttgagccc
     5161 aaggcagagc tggagctggc gccacccaga cagcgtcagg tgtggctggg gtaggtttgg
     5221 aggtctgcca gttacaccaa gtcccctctg agattcgatc aggggactgg atagattctt
     5281 tcaggtactc aatcaggaag ctggaggtgt tagacaccag ccccctgcat ccttcagtag
     5341 acctccctct gaacaccaca gccaggtcct gccttctggg ggcctgaata ttccagagct
     5401 gatgtgatgg gctgtgcaga agggggctgt atcaacatca attagggaac caaagttgca
     5461 ctatctgggc ccagattgtc tggttggcaa gagcaaagtt tccgttgatg aaacagacat
     5521 cccacaacaa aaacccaagt tttctgtgct acatgtgcaa tatttgttat gaatgttatc
     5581 acaagtcatt catcaagtta tctttataat cactgtagtt agatgtttca tgtccattca
     5641 agtgactttt attctgagtg caatatttca atagccttgt agtgataact agtgttgctt
     5701 ttgttttaga tgatctatgt gcagggcaat gcaatgaagt tgaaaaccct tgtaaatagg
     5761 agaggttgca aaccaaatca agagtattta ttactattac tgctattatt attaggcctg
     5821 cctttaattt tcagtgtaag tgttcagtat gccgcatcct gcctcagtat tgatcttgtg
     5881 ttctttgtgc caatatgaaa aggagagggt tggttctttc ctttattgtt gaatgctccc
     5941 atttaatgct ttatggcttt tactgtgtta cttttttaga ctcccgtctg cacaaaatgc
     6001 aataaaaata attttattat accctt
//
