LOCUS       NM_001126108            5540 bp    mRNA    linear   PRI 12-OCT-2024
DEFINITION  Homo sapiens solute carrier family 12 member 3 (SLC12A3),
            transcript variant 3, mRNA.
ACCESSION   NM_001126108
VERSION     NM_001126108.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5540)
  AUTHORS   Gamba,G.
  TITLE     From Fish Physiology to Human Disease: The Discovery of the NCC,
            NKCC2, and the Cation-Coupled Chloride Cotransporters
  JOURNAL   Kidney360 5 (1), 133-141 (2024)
   PUBMED   37968800
  REMARK    GeneRIF: From Fish Physiology to Human Disease: The Discovery of
            the NCC, NKCC2, and the Cation-Coupled Chloride Cotransporters.
REFERENCE   2  (bases 1 to 5540)
  AUTHORS   Mou,L., Tang,M., Zhu,L., Lin,W. and Gu,Y.
  TITLE     Spectrum of variants in a large Chinese Gitelman syndrome cohort
  JOURNAL   Clin Genet 104 (6), 674-678 (2023)
   PUBMED   37702302
  REMARK    GeneRIF: Spectrum of variants in a large Chinese Gitelman syndrome
            cohort.
REFERENCE   3  (bases 1 to 5540)
  AUTHORS   Bi,Y., Kuang,M.Y. and Li,M.L.
  TITLE     Novel heterozygous mutations of SLC12A3 gene in a Chinese pedigree
            with Gitelman syndrome: A care-compliant case report
  JOURNAL   Medicine (Baltimore) 102 (35), e34967 (2023)
   PUBMED   37657006
  REMARK    GeneRIF: Novel heterozygous mutations of SLC12A3 gene in a Chinese
            pedigree with Gitelman syndrome: A care-compliant case report.
REFERENCE   4  (bases 1 to 5540)
  AUTHORS   Yan,C., Hu,X., Liu,X., Zhao,J., Le,Z., Feng,J., Zhou,M., Ma,X.,
            Zheng,Q. and Sun,J.
  TITLE     Upregulation of SLC12A3 and SLC12A9 Mediated by the HCP5/miR-140-5p
            Axis Confers Aggressiveness and Unfavorable Prognosis in Uveal
            Melanoma
  JOURNAL   Lab Invest 103 (3), 100022 (2023)
   PUBMED   36925204
  REMARK    GeneRIF: Upregulation of SLC12A3 and SLC12A9 Mediated by the
            HCP5/miR-140-5p Axis Confers Aggressiveness and Unfavorable
            Prognosis in Uveal Melanoma.
REFERENCE   5  (bases 1 to 5540)
  AUTHORS   Fan,M., Zhang,J., Lee,C.L., Zhang,J. and Feng,L.
  TITLE     Structure and thiazide inhibition mechanism of the human Na-Cl
            cotransporter
  JOURNAL   Nature 614 (7949), 788-793 (2023)
   PUBMED   36792826
  REMARK    GeneRIF: Structure and thiazide inhibition mechanism of the human
            Na-Cl cotransporter.
REFERENCE   6  (bases 1 to 5540)
  AUTHORS   Takeuchi,K., Kure,S., Kato,T., Taniyama,Y., Takahashi,N., Ikeda,Y.,
            Abe,T., Narisawa,K., Muramatsu,Y. and Abe,K.
  TITLE     Association of a mutation in thiazide-sensitive Na-Cl cotransporter
            with familial Gitelman's syndrome
  JOURNAL   J Clin Endocrinol Metab 81 (12), 4496-4499 (1996)
   PUBMED   8954067
REFERENCE   7  (bases 1 to 5540)
  AUTHORS   Mastroianni,N., Bettinelli,A., Bianchetti,M., Colussi,G., De
            Fusco,M., Sereni,F., Ballabio,A. and Casari,G.
  TITLE     Novel molecular variants of the Na-Cl cotransporter gene are
            responsible for Gitelman syndrome
  JOURNAL   Am J Hum Genet 59 (5), 1019-1026 (1996)
   PUBMED   8900229
REFERENCE   8  (bases 1 to 5540)
  AUTHORS   Mastroianni,N., De Fusco,M., Zollo,M., Arrigo,G., Zuffardi,O.,
            Bettinelli,A., Ballabio,A. and Casari,G.
  TITLE     Molecular cloning, expression pattern, and chromosomal localization
            of the human Na-Cl thiazide-sensitive cotransporter (SLC12A3)
  JOURNAL   Genomics 35 (3), 486-493 (1996)
   PUBMED   8812482
REFERENCE   9  (bases 1 to 5540)
  AUTHORS   Chang,H., Tashiro,K., Hirai,M., Ikeda,K., Kurokawa,K. and Fujita,T.
  TITLE     Identification of a cDNA encoding a thiazide-sensitive
            sodium-chloride cotransporter from the human and its mRNA
            expression in various tissues
  JOURNAL   Biochem Biophys Res Commun 223 (2), 324-328 (1996)
   PUBMED   8670281
REFERENCE   10 (bases 1 to 5540)
  AUTHORS   Simon,D.B., Nelson-Williams,C., Bia,M.J., Ellison,D., Karet,F.E.,
            Molina,A.M., Vaara,I., Iwata,F., Cushner,H.M., Koolen,M.,
            Gainza,F.J., Gitleman,H.J. and Lifton,R.P.
  TITLE     Gitelman's variant of Bartter's syndrome, inherited hypokalaemic
            alkalosis, is caused by mutations in the thiazide-sensitive Na-Cl
            cotransporter
  JOURNAL   Nat Genet 12 (1), 24-30 (1996)
   PUBMED   8528245
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC012181.8.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_001126108.1.
            
            Summary: This gene encodes a renal thiazide-sensitive
            sodium-chloride cotransporter that is important for electrolyte
            homeostasis. This cotransporter mediates sodium and chloride
            reabsorption in the distal convoluted tubule. Mutations in this
            gene cause Gitelman syndrome, a disease similar to Bartter's
            syndrome, that is characterized by hypokalemic alkalosis combined
            with hypomagnesemia, low urinary calcium, and increased renin
            activity associated with normal blood pressure. This cotransporter
            is the target for thiazide diuretics that are used for treating
            high blood pressure. Multiple transcript variants encoding
            different isoforms have been found for this gene. [provided by
            RefSeq, Jul 2008].
            
            Transcript Variant: This variant (3) uses an alternate in-frame
            splice site in the 3' coding region, compared to variant 1,
            resulting in a protein (isoform 3) that is 9 aa shorter than
            isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X91220.1, SRR14038192.3017567.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1970526, SAMEA2145774
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000563236.6/ ENSP00000456149.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-311               AC012181.8         102375-102685       c
            312-458             AC012181.8         100676-100822       c
            459-534             AC012181.8         99520-99595         c
            535-630             AC012181.8         98068-98163         c
            631-770             AC012181.8         97657-97796         c
            771-881             AC012181.8         97156-97266         c
            882-993             AC012181.8         95430-95541         c
            994-1124            AC012181.8         95106-95236         c
            1125-1209           AC012181.8         89731-89815         c
            1210-1364           AC012181.8         88665-88819         c
            1365-1472           AC012181.8         88243-88350         c
            1473-1596           AC012181.8         87639-87762         c
            1597-1698           AC012181.8         85395-85496         c
            1699-1854           AC012181.8         83688-83843         c
            1855-1954           AC012181.8         82528-82627         c
            1955-2066           AC012181.8         81417-81528         c
            2067-2207           AC012181.8         80799-80939         c
            2208-2314           AC012181.8         79861-79967         c
            2315-2397           AC012181.8         77536-77618         c
            2398-2448           AC012181.8         75759-75809         c
            2449-2550           AC012181.8         74838-74939         c
            2551-2662           AC012181.8         73250-73361         c
            2663-2749           AC012181.8         68276-68362         c
            2750-2885           AC012181.8         65384-65519         c
            2886-2953           AC012181.8         63430-63497         c
            2954-5540           AC012181.8         52042-54628         c
FEATURES             Location/Qualifiers
     source          1..5540
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16q13"
     gene            1..5540
                     /gene="SLC12A3"
                     /gene_synonym="NCC; NCCT; TSC"
                     /note="solute carrier family 12 member 3"
                     /db_xref="GeneID:6559"
                     /db_xref="HGNC:HGNC:10912"
                     /db_xref="MIM:600968"
     exon            1..311
                     /gene="SLC12A3"
                     /gene_synonym="NCC; NCCT; TSC"
                     /inference="alignment:Splign:2.1.0"
     CDS             30..3095
                     /gene="SLC12A3"
                     /gene_synonym="NCC; NCCT; TSC"
                     /note="isoform 3 is encoded by transcript variant 3;
                     thiazide-sensitive Na-Cl cotransporter; Na-Cl symporter;
                     NaCl electroneutral thiazide-sensitive cotransporter;
                     thiazide-sensitive sodium-chloride cotransporter; solute
                     carrier family 12 (sodium/chloride transporter), member 3;
                     sodium-chloride co-transporter"
                     /codon_start=1
                     /product="solute carrier family 12 member 3 isoform 3"
                     /protein_id="NP_001119580.2"
                     /db_xref="CCDS:CCDS58464.1"
                     /db_xref="GeneID:6559"
                     /db_xref="HGNC:HGNC:10912"
                     /db_xref="MIM:600968"
                     /translation="MAELPTTETPGDATLCSGRFTISTLLSSDEPSPPAAYDSSHPSH
                     LTHSSTFCMRTFGYNTIDVVPTYEHYANSTQPGEPRKVRPTLADLHSFLKQEGRHLHA
                     LAFDSRPSHEMTDGLVEGEAGTSSEKNPEEPVRFGWVKGVMIRCMLNIWGVILYLRLP
                     WITAQAGIVLTWIIILLSVTVTSITGLSISAISTNGKVKSGGTYFLISRSLGPELGGS
                     IGLIFAFANAVGVAMHTVGFAETVRDLLQEYGAPIVDPINDIRIIAVVSVTVLLAISL
                     AGMEWESKAQVLFFLVIMVSFANYLVGTLIPPSEDKASKGFFSYRADIFVQNLVPDWR
                     GPDGTFFGMFSIFFPSATGILAGANISGDLKDPAIAIPKGTLMAIFWTTISYLAISAT
                     IGSCVVRDASGVLNDTVTPGWGACEGLACSYGWNFTECTQQHSCHYGLINYYQTMSMV
                     SGFAPLITAGIFGATLSSALACLVSAAKVFQCLCEDQLYPLIGFFGKGYGKNKEPVRG
                     YLLAYAIAVAFIIIAELNTIAPIISNFFLCSYALINFSCFHASITNSPGWRPSFQYYN
                     KWAALFGAIISVVIMFLLTWWAALIAIGVVLFLLLYVIYKKPEVNWGSSVQAGSYNLA
                     LSYSVGLNEVEDHIKNYRPQCLVLTGPPNFRPALVDFVGTFTRNLSLMICGHVLIGPH
                     KQRMPELQLIANGHTKWLNKRKIKAFYSDVIAEDLRRGVQILMQAAGLGRMKPNILVV
                     GFKKNWQSAHPATVEDYIGILHDAFDFNYGVCVMRMREGLNVSKMMQAHINPVFDPAE
                     DGKEASARVDPKALVKEEQATTIFQSEQGKKTIDIYWLFDDGGLTLLIPYLLGRKRRW
                     SKCKIRVFVGGQINRMDQERKAIISLLSKFRLGFHEVHILPDINQNPRAEHTKRFEDM
                     IAPFRLNDGFKDEATVNEMRRDCPWKISDEEITKNRVKSLRQVRLNEIVLDYSRDAAL
                     IVITLPIGRKGKCPSSLYMAWLETLSQDLRPPVILIRGNQENVLTFYCQ"
     exon            312..458
                     /gene="SLC12A3"
                     /gene_synonym="NCC; NCCT; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            459..534
                     /gene="SLC12A3"
                     /gene_synonym="NCC; NCCT; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            535..630
                     /gene="SLC12A3"
                     /gene_synonym="NCC; NCCT; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            631..770
                     /gene="SLC12A3"
                     /gene_synonym="NCC; NCCT; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            771..881
                     /gene="SLC12A3"
                     /gene_synonym="NCC; NCCT; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            882..993
                     /gene="SLC12A3"
                     /gene_synonym="NCC; NCCT; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            994..1124
                     /gene="SLC12A3"
                     /gene_synonym="NCC; NCCT; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            1125..1209
                     /gene="SLC12A3"
                     /gene_synonym="NCC; NCCT; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            1210..1364
                     /gene="SLC12A3"
                     /gene_synonym="NCC; NCCT; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            1365..1472
                     /gene="SLC12A3"
                     /gene_synonym="NCC; NCCT; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            1473..1596
                     /gene="SLC12A3"
                     /gene_synonym="NCC; NCCT; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            1597..1698
                     /gene="SLC12A3"
                     /gene_synonym="NCC; NCCT; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            1699..1854
                     /gene="SLC12A3"
                     /gene_synonym="NCC; NCCT; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            1855..1954
                     /gene="SLC12A3"
                     /gene_synonym="NCC; NCCT; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            1955..2066
                     /gene="SLC12A3"
                     /gene_synonym="NCC; NCCT; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            2067..2207
                     /gene="SLC12A3"
                     /gene_synonym="NCC; NCCT; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            2208..2314
                     /gene="SLC12A3"
                     /gene_synonym="NCC; NCCT; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            2315..2397
                     /gene="SLC12A3"
                     /gene_synonym="NCC; NCCT; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            2398..2448
                     /gene="SLC12A3"
                     /gene_synonym="NCC; NCCT; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            2449..2550
                     /gene="SLC12A3"
                     /gene_synonym="NCC; NCCT; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            2551..2662
                     /gene="SLC12A3"
                     /gene_synonym="NCC; NCCT; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            2663..2749
                     /gene="SLC12A3"
                     /gene_synonym="NCC; NCCT; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            2750..2885
                     /gene="SLC12A3"
                     /gene_synonym="NCC; NCCT; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            2886..2953
                     /gene="SLC12A3"
                     /gene_synonym="NCC; NCCT; TSC"
                     /inference="alignment:Splign:2.1.0"
     exon            2954..5540
                     /gene="SLC12A3"
                     /gene_synonym="NCC; NCCT; TSC"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      4216
                     /gene="SLC12A3"
                     /gene_synonym="NCC; NCCT; TSC"
                     /note="major polyA site"
     regulatory      5510..5515
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC12A3"
                     /gene_synonym="NCC; NCCT; TSC"
                     /note="hexamer: AATAAA"
     polyA_site      5540
                     /gene="SLC12A3"
                     /gene_synonym="NCC; NCCT; TSC"
ORIGIN      
        1 ctggcccctc cctggacacc caggcgacaa tggcagaact gcccacaaca gagacgcctg
       61 gggacgccac tttgtgcagc gggcgcttca ccatcagcac actgctgagc agtgatgagc
      121 cctctccacc agctgcctat gacagcagcc accccagcca cctgacccac agcagcacct
      181 tctgcatgcg cacctttggc tacaacacga tcgatgtggt gcccacatat gagcactatg
      241 ccaacagcac ccagcctggt gagccccgga aggtccggcc cacactggct gacctgcact
      301 ccttcctcaa gcaggaaggc agacacctgc atgccctggc ctttgacagc cggcccagcc
      361 acgagatgac tgatgggctg gtggagggcg aggcaggcac cagcagcgag aagaaccccg
      421 aggagccagt gcgcttcggc tgggtcaagg gggtgatgat tcgttgcatg ctcaacattt
      481 ggggcgtgat cctctacctg cggctgccct ggattacggc ccaggcaggc atcgtcctga
      541 cctggatcat catcctgctg tcggtcacgg tgacctccat cacaggcctc tccatctcag
      601 ccatctccac caatggcaag gtcaagtcag gtggcaccta cttcctcatc tcccggagtc
      661 tgggcccaga gcttgggggc tccatcggcc tcattttcgc tttcgccaat gccgtgggtg
      721 tggccatgca cacggtgggc tttgcagaga ccgtgcggga cctgctccag gagtatgggg
      781 cacccatcgt ggaccccatt aacgacatcc gcatcattgc cgtggtctcg gtcactgtgc
      841 tgctggccat ctccctggct ggcatggagt gggagtccaa ggcccaggtg ctgttcttcc
      901 ttgtcatcat ggtctccttt gccaactatt tagtggggac gctgatcccc ccatctgagg
      961 acaaggcctc caaaggcttc ttcagctacc gggcggacat ttttgtccag aacttggtgc
     1021 ctgactggcg gggtccagat ggcaccttct tcggaatgtt ctccatcttc ttcccctcgg
     1081 ccacaggcat cctggcaggg gccaacatat ctggtgacct caaggaccct gctatagcca
     1141 tccccaaggg gaccctcatg gccattttct ggacgaccat ttcctacctg gccatctcag
     1201 ccaccattgg ctcctgcgtg gtgcgtgatg cctctggggt cctgaatgac acagtgaccc
     1261 ctggctgggg tgcctgcgag gggctggcct gcagctatgg ctggaacttc accgagtgca
     1321 cccagcagca cagctgccac tacggcctca tcaactatta ccagaccatg agcatggtgt
     1381 caggcttcgc gcccctgatc acggctggca tcttcggggc caccctctcc tctgccctgg
     1441 cctgccttgt ctctgctgcc aaagtcttcc agtgcctttg cgaggaccag ctgtacccac
     1501 tgatcggctt cttcggcaaa ggctatggca agaacaagga gcccgtgcgt ggctacctgc
     1561 tggcctacgc catcgctgtg gccttcatca tcatcgctga gctcaacacc atagccccca
     1621 tcatttccaa cttcttcctc tgctcctatg ccctcatcaa cttcagctgc ttccacgcct
     1681 ccatcaccaa ctcgcctggg tggagacctt cattccaata ctacaacaag tgggcggcgc
     1741 tgtttggggc tatcatctcc gtggtcatca tgttcctcct cacctggtgg gcggccctca
     1801 tcgccattgg cgtggtgctc ttcctcctgc tctatgtcat ctacaagaag ccagaggtaa
     1861 attggggctc ctcggtacag gctggctcct acaacctggc cctcagctac tcggtgggcc
     1921 tcaatgaggt ggaagaccac atcaagaact accgccccca gtgcctggtg ctcacggggc
     1981 cccccaactt ccgcccggcc ctggtggact ttgtgggcac cttcacccgg aacctcagcc
     2041 tgatgatctg tggccacgtg ctcatcggac cccacaagca gaggatgcct gagctccagc
     2101 tcatcgccaa cgggcacacc aagtggctga acaagaggaa gatcaaggcc ttctactcgg
     2161 atgtcattgc cgaggacctc cgcagaggcg tccagatcct catgcaggcc gcaggtctcg
     2221 ggagaatgaa gcccaacatt ctggtggttg ggttcaagaa gaactggcag tcggctcacc
     2281 cggccacagt ggaagactac attggcatcc tccatgatgc ctttgatttc aactatggcg
     2341 tgtgtgtcat gaggatgcgg gagggactca acgtgtccaa gatgatgcag gcgcacatta
     2401 accccgtgtt tgacccagcg gaggacggga aggaagccag cgccagagtg gaccccaagg
     2461 ccctggtgaa ggaggagcag gccaccacca tcttccagtc ggagcagggc aagaagacca
     2521 tagacatcta ctggctcttt gacgatggag gcctcaccct cctcattccc tatctccttg
     2581 gccgcaagag gaggtggagc aaatgcaaga tccgtgtgtt cgtaggcggc cagattaaca
     2641 ggatggacca ggagagaaag gcgatcattt ctctgctgag caagttccga ctgggattcc
     2701 atgaagtcca catcctccct gacatcaacc agaaccctcg ggctgagcac accaagaggt
     2761 ttgaggacat gattgcaccc ttccgtctga atgatggctt caaggatgag gccactgtca
     2821 acgagatgcg gcgggactgc ccctggaaga tctcagatga ggagattacg aagaacagag
     2881 tcaagtccct tcggcaggtg aggctgaatg agattgtgct ggattactcc cgagacgctg
     2941 ctctcatcgt catcactttg cccataggga ggaaggggaa gtgccccagc tcgctgtaca
     3001 tggcctggct ggagaccctg tcccaggacc tcagacctcc agtcatcctg atccgaggaa
     3061 accaggaaaa cgtgctcacc ttttactgcc agtaactcca ggctttgaca tccctgtcca
     3121 cagctctgag tgtgtgggat aagttggaac ttgattgcct ctagtccaca gggatgagac
     3181 tcatgttctg ttgcacttta agtggcagca tctgatgatc tcaccgaaaa agatggtaga
     3241 tttccaaatc tggctggact ccacttccat gggacacatt ccctgggtct tgtgtttata
     3301 ggctagagaa atagcagatg gagctgcaag gaaaactctc taaagcatcc tattcctttt
     3361 aaaggatttc ttttgatttt gatgaccatt aattaagagt tcagtctttg atttgtatgc
     3421 aaattggagt cccaatgctg ggcgtgaatc ttgacagttt ctacagacct tcctgggtga
     3481 aagttcctaa atcatgccct gcttcctcca ataggagaat gggagcctca cctgtaggac
     3541 ctacaggctc tctaaggaat gcaggtctct ctctgagcct ccacagccag gcaaatacat
     3601 atatatatat ttttttttta gatgaagttt tttctcttgt tgcccaggct agggtgtaat
     3661 ggcatgatct caggtcactg caacctcctc ccgggttcaa gcatttcttc tgtctcagcc
     3721 tcccgaatag ctgggattac aggcacctgc catcacacga gctaattttt gtatttttag
     3781 tagagatggg gtttcaccat gttgaccagg ctggtgttga gctcctgacc tcaggtgatc
     3841 cacccacctc ggtctcccaa agtgctgggg ttacaggcct gagccactgc gcccggccca
     3901 ggcaaatttc ttgaaccact tctcactccc gtcactttca ataaggggtc tttgatgtct
     3961 tcactggttc tttggacgag ggacttttcg aacttttttg gttgcaacac acagtaagaa
     4021 atatacttca cactgagact tgcagcgcac acacacggaa acgaccaaaa caaaaatgtc
     4081 acaaaacaat acttaccctt ccctggggga cgtcctccag tatgttctgt tctgtttatt
     4141 tttcactgtt ggttgcaatc caataaaatg actttgggat ccactcatgg gtggggaccc
     4201 acacatttga aaggcatggc cacctttctg ttgtgccttg catttgtcca cacacaggga
     4261 gtctggctga gctggggaaa ggccacggct gggtgtcatt gccattttcc cagctcatct
     4321 caccgggaag aaaagcagat tgacagaaca cgtgaggagg ggtattgatg gcaggagagt
     4381 caaaaaagag ttttaaagaa ggggcaaggt tgaaggagtc tagtggcaag ggtaagattt
     4441 caggcatggt taagaacaga cgacaaggat gtcaggaatg aagatgtgga gaggggtgta
     4501 gagatggcaa ggttggcaag gaacagatag gcaggagcag gtccaagcca agcctagccc
     4561 aagaccaggt gaaaggagag gggaggagga gccacctgca agagatggaa agagcaggcg
     4621 gcagaggggg ctggcaggga ggggctgtta agagtggggt tggaggtggg agagaagcta
     4681 ggacaaggga gatggagaaa ggacctatac ctggctcacg gaaggccttc aggtcactac
     4741 acgttgaaca tccccagtgt ttgagccccc aaagctaggg tgcaagagca ctgccatcga
     4801 atgccagtgg gtgaggccaa gtgagggtat ttgcagctct agacataacc aagaagcgta
     4861 aaggtgagtt gtttggtggt acgactgcct gtgccttctt ccgatggcac tggggtggct
     4921 gaaggaacag acatctttgg gtttcatcag cctcctccaa gactgctgca gtgcctacac
     4981 tttagacttc agaaggagac taaagacttc tagaatttag aaggagatct gaagtctcct
     5041 ttctggagtt acaacccaaa ggatgttagc atttctcagg tcatcccact gcaaagccca
     5101 gaaggcttgg ggctcccagg ctgctctgaa gccccactgt ctgaccgcct cagggcttgc
     5161 tacgagggac tggggcacgg ccaagctgac taggaacagc tctcgtgctc ctgagggacc
     5221 tggaggatgg gcctgcctcc cagccattga gctggattct gggataattc ttaactcgaa
     5281 ataaggggaa gcatccatca gggaatgctg gcctttctag agccacgtag aaaacaattt
     5341 tctggttctt caaacctcaa agagtccttg gtccaaaaaa cagaatgttt tggcttcggg
     5401 tgtcaaaaaa aaaattttca cgatgtcaga aatagtatgt ttttaacaat agtaatagct
     5461 ttgtaaaaaa ataaaaagct ttaacagcga ggccataaac aatgaaatga ataaaaacgg
     5521 tggtcattca gtcaacggaa
//
