LOCUS       NM_001046               6874 bp    mRNA    linear   PRI 20-OCT-2024
DEFINITION  Homo sapiens solute carrier family 12 member 2 (SLC12A2),
            transcript variant 1, mRNA.
ACCESSION   NM_001046
VERSION     NM_001046.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6874)
  AUTHORS   Chen,D.Y., Zhang,Y.Y., Nie,H.H., Wang,H.Z., Qiu,P.S., Wang,F.,
            Peng,Y.N., Xu,F., Zhao,Q. and Zhang,M.
  TITLE     Comprehensive analyses of solute carrier family members identify
            SLC12A2 as a novel therapy target for colorectal cancer
  JOURNAL   Sci Rep 14 (1), 4459 (2024)
   PUBMED   38396064
  REMARK    GeneRIF: Comprehensive analyses of solute carrier family members
            identify SLC12A2 as a novel therapy target for colorectal cancer.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6874)
  AUTHORS   Neumann,C., Rosenbaek,L.L., Flygaard,R.K., Habeck,M., Karlsen,J.L.,
            Wang,Y., Lindorff-Larsen,K., Gad,H.H., Hartmann,R., Lyons,J.A.,
            Fenton,R.A. and Nissen,P.
  TITLE     Cryo-EM structure of the human NKCC1 transporter reveals mechanisms
            of ion coupling and specificity
  JOURNAL   EMBO J 41 (23), e110169 (2022)
   PUBMED   36239040
  REMARK    GeneRIF: Cryo-EM structure of the human NKCC1 transporter reveals
            mechanisms of ion coupling and specificity.
REFERENCE   3  (bases 1 to 6874)
  AUTHORS   Li,Y., Li,Y.L., Liu,Y.L., Fu,J., Zhang,W.W. and Piao,Y.S.
  TITLE     [Expression of cation chloride cotransporter (NKCC1/KCC2) in brain
            tissue of children with focal cortical dysplasia type ]
  JOURNAL   Zhonghua Bing Li Xue Za Zhi 51 (11), 1123-1128 (2022)
   PUBMED   36323541
  REMARK    GeneRIF: [Expression of cation chloride cotransporter (NKCC1/KCC2)
            in brain tissue of children with focal cortical dysplasia type ].
REFERENCE   4  (bases 1 to 6874)
  AUTHORS   Koumangoye,R., Penny,P. and Delpire,E.
  TITLE     Loss of NKCC1 function increases epithelial tight junction
            permeability by upregulating claudin-2 expression
  JOURNAL   Am J Physiol Cell Physiol 323 (4), C1251-C1263 (2022)
   PUBMED   35968893
  REMARK    GeneRIF: Loss of NKCC1 function increases epithelial tight junction
            permeability by upregulating claudin-2 expression.
REFERENCE   5  (bases 1 to 6874)
  AUTHORS   Mohaghghegh,H., Ananloo,E.S., Hadjighasem,M., Karimipour,M.,
            Hashemizadeh,S. and Abhari,S.A.A.
  TITLE     NKCC1 to KCC2 mRNA Ratio in Schizophrenia and Its Psychopathology:
            a Case-Control Study
  JOURNAL   J Mol Neurosci 72 (8), 1670-1681 (2022)
   PUBMED   35624355
  REMARK    GeneRIF: NKCC1 to KCC2 mRNA Ratio in Schizophrenia and Its
            Psychopathology: a Case-Control Study.
REFERENCE   6  (bases 1 to 6874)
  AUTHORS   Moore-Hoon,M.L. and Turner,R.J.
  TITLE     The structural unit of the secretory Na+-K+-2Cl- cotransporter
            (NKCC1) is a homodimer
  JOURNAL   Biochemistry 39 (13), 3718-3724 (2000)
   PUBMED   10736171
REFERENCE   7  (bases 1 to 6874)
  AUTHORS   Haas,M. and Forbush,B. 3rd.
  TITLE     The Na-K-Cl cotransporters
  JOURNAL   J Bioenerg Biomembr 30 (2), 161-172 (1998)
   PUBMED   9672238
  REMARK    Review article
REFERENCE   8  (bases 1 to 6874)
  AUTHORS   Gress,T.M., Muller-Pillasch,F., Geng,M., Zimmerhackl,F.,
            Zehetner,G., Friess,H., Buchler,M., Adler,G. and Lehrach,H.
  TITLE     A pancreatic cancer-specific expression profile
  JOURNAL   Oncogene 13 (8), 1819-1830 (1996)
   PUBMED   8895530
REFERENCE   9  (bases 1 to 6874)
  AUTHORS   Gillen,C.M., Brill,S., Payne,J.A. and Forbush,B. 3rd.
  TITLE     Molecular cloning and functional expression of the K-Cl
            cotransporter from rabbit, rat, and human. A new member of the
            cation-chloride cotransporter family
  JOURNAL   J Biol Chem 271 (27), 16237-16244 (1996)
   PUBMED   8663127
REFERENCE   10 (bases 1 to 6874)
  AUTHORS   Payne,J.A., Xu,J.C., Haas,M., Lytle,C.Y., Ward,D. and Forbush,B.
            3rd.
  TITLE     Primary structure, functional expression, and chromosomal
            localization of the bumetanide-sensitive Na-K-Cl cotransporter in
            human colon
  JOURNAL   J Biol Chem 270 (30), 17977-17985 (1995)
   PUBMED   7629105
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC011332.8, AY280459.1,
            U30246.1, BC033003.2, AK025062.1, AA425619.1, AA425438.1,
            AA843086.1, AV708692.2 and BM978607.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_001046.2.
            
            Summary: The protein encoded by this gene mediates sodium and
            chloride transport and reabsorption. The encoded protein is a
            membrane protein and is important in maintaining proper ionic
            balance and cell volume. This protein is phosphorylated in response
            to DNA damage. Three transcript variants encoding two different
            isoforms have been found for this gene. [provided by RefSeq, Jan
            2012].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY280459.1, SRR1660803.193764.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000262461.7/ ENSP00000262461.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-25                AC011332.8         104090-104114       c
            26-1379             AY280459.1         26-1379
            1380-3884           U30246.1           1355-3859
            3885-4222           BC033003.2         3926-4263
            4223-5313           AK025062.1         25-1115
            5314-5485           AA425619.1         331-502
            5486-5522           AA425438.1         412-448             c
            5523-5820           AA843086.1         23-320
            5821-6118           AV708692.2         30-327
            6119-6404           AV708692.2         329-614
            6405-6874           BM978607.1         18-487              c
FEATURES             Location/Qualifiers
     source          1..6874
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q23.3"
     gene            1..6874
                     /gene="SLC12A2"
                     /gene_synonym="BSC; BSC-2; BSC2; CCC1; hNKCC1; KILQS;
                     NKCC1; PPP1R141"
                     /note="solute carrier family 12 member 2"
                     /db_xref="GeneID:6558"
                     /db_xref="HGNC:HGNC:10911"
                     /db_xref="MIM:600840"
     exon            1..945
                     /gene="SLC12A2"
                     /gene_synonym="BSC; BSC-2; BSC2; CCC1; hNKCC1; KILQS;
                     NKCC1; PPP1R141"
                     /inference="alignment:Splign:2.1.0"
     CDS             190..3828
                     /gene="SLC12A2"
                     /gene_synonym="BSC; BSC-2; BSC2; CCC1; hNKCC1; KILQS;
                     NKCC1; PPP1R141"
                     /note="isoform 1 is encoded by transcript variant 1;
                     bumetanide-sensitive sodium-(potassium)-chloride
                     cotransporter 1; basolateral Na-K-Cl symporter; solute
                     carrier family 12 (sodium/potassium/chloride
                     transporters), member 2; protein phosphatase 1, regulatory
                     subunit 141; solute carrier family 12
                     (sodium/potassium/chloride transporter), member 2;
                     Na-K-2Cl cotransporter 1; bumetanide-sensitive
                     sodium-(potassium)-chloride cotransporter 2;
                     bumetanide-sensitive cotransporter type 2"
                     /codon_start=1
                     /product="solute carrier family 12 member 2 isoform 1"
                     /protein_id="NP_001037.1"
                     /db_xref="CCDS:CCDS4144.1"
                     /db_xref="GeneID:6558"
                     /db_xref="HGNC:HGNC:10911"
                     /db_xref="MIM:600840"
                     /translation="MEPRPTAPSSGAPGLAGVGETPSAAALAAARVELPGTAVPSVPE
                     DAAPASRDGGGVRDEGPAAAGDGLGRPLGPTPSQSRFQVDLVSENAGRAAAAAAAAAA
                     AAAAAGAGAGAKQTPADGEASGESEPAKGSEEAKGRFRVNFVDPAASSSAEDSLSDAA
                     GVGVDGPNVSFQNGGDTVLSEGSSLHSGGGGGSGHHQHYYYDTHTNTYYLRTFGHNTM
                     DAVPRIDHYRHTAAQLGEKLLRPSLAELHDELEKEPFEDGFANGEESTPTRDAVVTYT
                     AESKGVVKFGWIKGVLVRCMLNIWGVMLFIRLSWIVGQAGIGLSVLVIMMATVVTTIT
                     GLSTSAIATNGFVRGGGAYYLISRSLGPEFGGAIGLIFAFANAVAVAMYVVGFAETVV
                     ELLKEHSILMIDEINDIRIIGAITVVILLGISVAGMEWEAKAQIVLLVILLLAIGDFV
                     IGTFIPLESKKPKGFFGYKSEIFNENFGPDFREEETFFSVFAIFFPAATGILAGANIS
                     GDLADPQSAIPKGTLLAILITTLVYVGIAVSVGSCVVRDATGNVNDTIVTELTNCTSA
                     ACKLNFDFSSCESSPCSYGLMNNFQVMSMVSGFTPLISAGIFSATLSSALASLVSAPK
                     IFQALCKDNIYPAFQMFAKGYGKNNEPLRGYILTFLIALGFILIAELNVIAPIISNFF
                     LASYALINFSVFHASLAKSPGWRPAFKYYNMWISLLGAILCCIVMFVINWWAALLTYV
                     IVLGLYIYVTYKKPDVNWGSSTQALTYLNALQHSIRLSGVEDHVKNFRPQCLVMTGAP
                     NSRPALLHLVHDFTKNVGLMICGHVHMGPRRQAMKEMSIDQAKYQRWLIKNKMKAFYA
                     PVHADDLREGAQYLMQAAGLGRMKPNTLVLGFKKDWLQADMRDVDMYINLFHDAFDIQ
                     YGVVVIRLKEGLDISHLQGQEELLSSQEKSPGTKDVVVSVEYSKKSDLDTSKPLSEKP
                     ITHKVEEEDGKTATQPLLKKESKGPIVPLNVADQKLLEASTQFQKKQGKNTIDVWWLF
                     DDGGLTLLIPYLLTTKKKWKDCKIRVFIGGKINRIDHDRRAMATLLSKFRIDFSDIMV
                     LGDINTKPKKENIIAFEEIIEPYRLHEDDKEQDIADKMKEDEPWRITDNELELYKTKT
                     YRQIRLNELLKEHSSTANIIVMSLPVARKGAVSSALYMAWLEALSKDLPPILLVRGNH
                     QSVLTFYS"
     exon            946..1065
                     /gene="SLC12A2"
                     /gene_synonym="BSC; BSC-2; BSC2; CCC1; hNKCC1; KILQS;
                     NKCC1; PPP1R141"
                     /inference="alignment:Splign:2.1.0"
     exon            1066..1141
                     /gene="SLC12A2"
                     /gene_synonym="BSC; BSC-2; BSC2; CCC1; hNKCC1; KILQS;
                     NKCC1; PPP1R141"
                     /inference="alignment:Splign:2.1.0"
     exon            1142..1237
                     /gene="SLC12A2"
                     /gene_synonym="BSC; BSC-2; BSC2; CCC1; hNKCC1; KILQS;
                     NKCC1; PPP1R141"
                     /inference="alignment:Splign:2.1.0"
     exon            1238..1377
                     /gene="SLC12A2"
                     /gene_synonym="BSC; BSC-2; BSC2; CCC1; hNKCC1; KILQS;
                     NKCC1; PPP1R141"
                     /inference="alignment:Splign:2.1.0"
     exon            1378..1488
                     /gene="SLC12A2"
                     /gene_synonym="BSC; BSC-2; BSC2; CCC1; hNKCC1; KILQS;
                     NKCC1; PPP1R141"
                     /inference="alignment:Splign:2.1.0"
     exon            1489..1597
                     /gene="SLC12A2"
                     /gene_synonym="BSC; BSC-2; BSC2; CCC1; hNKCC1; KILQS;
                     NKCC1; PPP1R141"
                     /inference="alignment:Splign:2.1.0"
     exon            1598..1725
                     /gene="SLC12A2"
                     /gene_synonym="BSC; BSC-2; BSC2; CCC1; hNKCC1; KILQS;
                     NKCC1; PPP1R141"
                     /inference="alignment:Splign:2.1.0"
     exon            1726..1810
                     /gene="SLC12A2"
                     /gene_synonym="BSC; BSC-2; BSC2; CCC1; hNKCC1; KILQS;
                     NKCC1; PPP1R141"
                     /inference="alignment:Splign:2.1.0"
     exon            1811..1962
                     /gene="SLC12A2"
                     /gene_synonym="BSC; BSC-2; BSC2; CCC1; hNKCC1; KILQS;
                     NKCC1; PPP1R141"
                     /inference="alignment:Splign:2.1.0"
     exon            1963..2070
                     /gene="SLC12A2"
                     /gene_synonym="BSC; BSC-2; BSC2; CCC1; hNKCC1; KILQS;
                     NKCC1; PPP1R141"
                     /inference="alignment:Splign:2.1.0"
     exon            2071..2194
                     /gene="SLC12A2"
                     /gene_synonym="BSC; BSC-2; BSC2; CCC1; hNKCC1; KILQS;
                     NKCC1; PPP1R141"
                     /inference="alignment:Splign:2.1.0"
     exon            2195..2296
                     /gene="SLC12A2"
                     /gene_synonym="BSC; BSC-2; BSC2; CCC1; hNKCC1; KILQS;
                     NKCC1; PPP1R141"
                     /inference="alignment:Splign:2.1.0"
     exon            2297..2452
                     /gene="SLC12A2"
                     /gene_synonym="BSC; BSC-2; BSC2; CCC1; hNKCC1; KILQS;
                     NKCC1; PPP1R141"
                     /inference="alignment:Splign:2.1.0"
     exon            2453..2552
                     /gene="SLC12A2"
                     /gene_synonym="BSC; BSC-2; BSC2; CCC1; hNKCC1; KILQS;
                     NKCC1; PPP1R141"
                     /inference="alignment:Splign:2.1.0"
     exon            2553..2664
                     /gene="SLC12A2"
                     /gene_synonym="BSC; BSC-2; BSC2; CCC1; hNKCC1; KILQS;
                     NKCC1; PPP1R141"
                     /inference="alignment:Splign:2.1.0"
     exon            2665..2805
                     /gene="SLC12A2"
                     /gene_synonym="BSC; BSC-2; BSC2; CCC1; hNKCC1; KILQS;
                     NKCC1; PPP1R141"
                     /inference="alignment:Splign:2.1.0"
     exon            2806..2912
                     /gene="SLC12A2"
                     /gene_synonym="BSC; BSC-2; BSC2; CCC1; hNKCC1; KILQS;
                     NKCC1; PPP1R141"
                     /inference="alignment:Splign:2.1.0"
     exon            2913..2992
                     /gene="SLC12A2"
                     /gene_synonym="BSC; BSC-2; BSC2; CCC1; hNKCC1; KILQS;
                     NKCC1; PPP1R141"
                     /inference="alignment:Splign:2.1.0"
     exon            2993..3118
                     /gene="SLC12A2"
                     /gene_synonym="BSC; BSC-2; BSC2; CCC1; hNKCC1; KILQS;
                     NKCC1; PPP1R141"
                     /inference="alignment:Splign:2.1.0"
     exon            3119..3166
                     /gene="SLC12A2"
                     /gene_synonym="BSC; BSC-2; BSC2; CCC1; hNKCC1; KILQS;
                     NKCC1; PPP1R141"
                     /inference="alignment:Splign:2.1.0"
     exon            3167..3289
                     /gene="SLC12A2"
                     /gene_synonym="BSC; BSC-2; BSC2; CCC1; hNKCC1; KILQS;
                     NKCC1; PPP1R141"
                     /inference="alignment:Splign:2.1.0"
     exon            3290..3401
                     /gene="SLC12A2"
                     /gene_synonym="BSC; BSC-2; BSC2; CCC1; hNKCC1; KILQS;
                     NKCC1; PPP1R141"
                     /inference="alignment:Splign:2.1.0"
     exon            3402..3488
                     /gene="SLC12A2"
                     /gene_synonym="BSC; BSC-2; BSC2; CCC1; hNKCC1; KILQS;
                     NKCC1; PPP1R141"
                     /inference="alignment:Splign:2.1.0"
     exon            3489..3624
                     /gene="SLC12A2"
                     /gene_synonym="BSC; BSC-2; BSC2; CCC1; hNKCC1; KILQS;
                     NKCC1; PPP1R141"
                     /inference="alignment:Splign:2.1.0"
     exon            3625..3692
                     /gene="SLC12A2"
                     /gene_synonym="BSC; BSC-2; BSC2; CCC1; hNKCC1; KILQS;
                     NKCC1; PPP1R141"
                     /inference="alignment:Splign:2.1.0"
     exon            3693..6874
                     /gene="SLC12A2"
                     /gene_synonym="BSC; BSC-2; BSC2; CCC1; hNKCC1; KILQS;
                     NKCC1; PPP1R141"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4201..4206
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC12A2"
                     /gene_synonym="BSC; BSC-2; BSC2; CCC1; hNKCC1; KILQS;
                     NKCC1; PPP1R141"
                     /note="hexamer: TATAAA"
     polyA_site      4222
                     /gene="SLC12A2"
                     /gene_synonym="BSC; BSC-2; BSC2; CCC1; hNKCC1; KILQS;
                     NKCC1; PPP1R141"
                     /note="major polyA site"
     regulatory      6847..6852
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC12A2"
                     /gene_synonym="BSC; BSC-2; BSC2; CCC1; hNKCC1; KILQS;
                     NKCC1; PPP1R141"
                     /note="hexamer: AATAAA"
     polyA_site      6874
                     /gene="SLC12A2"
                     /gene_synonym="BSC; BSC-2; BSC2; CCC1; hNKCC1; KILQS;
                     NKCC1; PPP1R141"
                     /note="major polyA site"
ORIGIN      
        1 acactcgcgc gctcgctcgg ctgccggtgg cctctgtggc cgtccaggct agcggcggcc
       61 cgcaggcggc ggggagaaag actctctcac ctggtcttgc ggctgtggcc accgccggcc
      121 aggggtgtgg agggcgtgct gccggagacg tccgccgggc tctgcagttc cgccgggggt
      181 cgggcagcta tggagccgcg gcccacggcg ccctcctccg gcgccccggg actggccggg
      241 gtcggggaga cgccgtcagc cgctgcgctg gccgcagcca gggtggaact gcccggcacg
      301 gctgtgccct cggtgccgga ggatgctgcg cccgcgagcc gggacggcgg cggggtccgc
      361 gatgagggcc ccgcggcggc cggggacggg ctgggcagac ccttggggcc caccccgagc
      421 cagagccgtt tccaggtgga cctggtttcc gagaacgccg ggcgggccgc tgctgcggcg
      481 gcggcggcgg cggcggcagc ggcggcggct ggtgctgggg cgggggccaa gcagaccccc
      541 gcggacgggg aagccagcgg cgagagcgag ccggctaaag gcagcgagga agccaagggc
      601 cgcttccgcg tgaacttcgt ggacccagct gcctcctcgt cggctgaaga cagcctgtca
      661 gatgctgccg gggtcggagt cgacgggccc aacgtgagct tccagaacgg cggggacacg
      721 gtgctgagcg agggcagcag cctgcactcc ggcggcggcg gcggcagtgg gcaccaccag
      781 cactactatt atgataccca caccaacacc tactacctgc gcaccttcgg ccacaacacc
      841 atggacgctg tgcccaggat cgatcactac cggcacacag ccgcgcagct gggcgagaag
      901 ctgctccggc ctagcctggc ggagctccac gacgagctgg aaaaggaacc ttttgaggat
      961 ggctttgcaa atggggaaga aagtactcca accagagatg ctgtggtcac gtatactgca
     1021 gaaagtaaag gagtcgtgaa gtttggctgg atcaagggtg tattagtacg ttgtatgtta
     1081 aacatttggg gtgtgatgct tttcattaga ttgtcatgga ttgtgggtca agctggaata
     1141 ggtctatcag tccttgtaat aatgatggcc actgttgtga caactatcac aggattgtct
     1201 acttcagcaa tagcaactaa tggatttgta agaggaggag gagcatatta tttaatatct
     1261 agaagtctag ggccagaatt tggtggtgca attggtctaa tcttcgcctt tgccaacgct
     1321 gttgcagttg ctatgtatgt ggttggattt gcagaaaccg tggtggagtt gcttaaggaa
     1381 cattccatac ttatgataga tgaaatcaat gatatccgaa ttattggagc cattacagtc
     1441 gtgattcttt taggtatctc agtagctgga atggagtggg aagcaaaagc tcagattgtt
     1501 cttttggtga tcctacttct tgctattggt gatttcgtca taggaacatt tatcccactg
     1561 gagagcaaga agccaaaagg gttttttggt tataaatctg aaatatttaa tgagaacttt
     1621 gggcccgatt ttcgagagga agagactttc ttttctgtat ttgccatctt ttttcctgct
     1681 gcaactggta ttctggctgg agcaaatatc tcaggtgatc ttgcagatcc tcagtcagcc
     1741 atacccaaag gaacactcct agccatttta attactacat tggtttacgt aggaattgca
     1801 gtatctgtag gttcttgtgt tgttcgagat gccactggaa acgttaatga cactatcgta
     1861 acagagctaa caaactgtac ttctgcagcc tgcaaattaa actttgattt ttcatcttgt
     1921 gaaagcagtc cttgttccta tggcctaatg aacaacttcc aggtaatgag tatggtgtca
     1981 ggatttacac cactaatttc tgcaggtata ttttcagcca ctctttcttc agcattagca
     2041 tccctagtga gtgctcccaa aatatttcag gctctatgta aggacaacat ctacccagct
     2101 ttccagatgt ttgctaaagg ttatgggaaa aataatgaac ctcttcgtgg ctacatctta
     2161 acattcttaa ttgcacttgg attcatctta attgctgaac tgaatgttat tgcaccaatt
     2221 atctcaaact tcttccttgc atcatatgca ttgatcaatt tttcagtatt ccatgcatca
     2281 cttgcaaaat ctccaggatg gcgtcctgca ttcaaatact acaacatgtg gatatcactt
     2341 cttggagcaa ttctttgttg catagtaatg ttcgtcatta actggtgggc tgcattgcta
     2401 acatatgtga tagtccttgg gctgtatatt tatgttacct acaaaaaacc agatgtgaat
     2461 tggggatcct ctacacaagc cctgacttac ctgaatgcac tgcagcattc aattcgtctt
     2521 tctggagtgg aagaccacgt gaaaaacttt aggccacagt gtcttgttat gacaggtgct
     2581 ccaaactcac gtccagcttt acttcatctt gttcatgatt tcacaaaaaa tgttggtttg
     2641 atgatctgtg gccatgtaca tatgggtcct cgaagacaag ccatgaaaga gatgtccatc
     2701 gatcaagcca aatatcagcg atggcttatt aagaacaaaa tgaaggcatt ttatgctcca
     2761 gtacatgcag atgacttgag agaaggtgca cagtatttga tgcaggctgc tggtcttggt
     2821 cgtatgaagc caaacacact tgtccttgga tttaagaaag attggttgca agcagatatg
     2881 agggatgtgg atatgtatat aaacttattt catgatgctt ttgacataca atatggagta
     2941 gtggttattc gcctaaaaga aggtctggat atatctcatc ttcaaggaca agaagaatta
     3001 ttgtcatcac aagagaaatc tcctggcacc aaggatgtgg tagtaagtgt ggaatatagt
     3061 aaaaagtccg atttagatac ttccaaacca ctcagtgaaa aaccaattac acacaaagtt
     3121 gaggaagagg atggcaagac tgcaactcaa ccactgttga aaaaagaatc caaaggccct
     3181 attgtgcctt taaatgtagc tgaccaaaag cttcttgaag ctagtacaca gtttcagaaa
     3241 aaacaaggaa agaatactat tgatgtctgg tggctttttg atgatggagg tttgacctta
     3301 ttgatacctt accttctgac gaccaagaaa aaatggaaag actgtaagat cagagtattc
     3361 attggtggaa agataaacag aatagaccat gaccggagag cgatggctac tttgcttagc
     3421 aagttccgga tagacttttc tgatatcatg gttctaggag atatcaatac caaaccaaag
     3481 aaagaaaata ttatagcttt tgaggaaatc attgagccat acagacttca tgaagatgat
     3541 aaagagcaag atattgcaga taaaatgaaa gaagatgaac catggcgaat aacagataat
     3601 gagcttgaac tttataagac caagacatac cggcagatca ggttaaatga gttattaaag
     3661 gaacattcaa gcacagctaa tattattgtc atgagtctcc cagttgcacg aaaaggtgct
     3721 gtgtctagtg ctctctacat ggcatggtta gaagctctat ctaaggacct accaccaatc
     3781 ctcctagttc gtgggaatca tcagagtgtc cttaccttct attcataaat gttctataca
     3841 gtggacagcc ctccagaatg gtacttcagt gcctagtgta gtaactgaaa tcttcaatga
     3901 cacattaaca tcacaatggc gaatggtgac ttttctttca cgatttcatt aatttgaaag
     3961 cacacaggaa agttgctcca ttgataacgt gtatggagac ttcggtttta gtcaattcca
     4021 tatctcaatc ttaatggtga ttcttctctg ttgaactgaa gtttgtgaga gtagttttcc
     4081 tttgctactt gaatagcaat aaaagcgtgt taactttttg attgatgaaa gaagtacaaa
     4141 aagcctttag ccttgaggtg ccttctgaaa ttaaccaaat ttcatccata tatcctcttt
     4201 tataaactta tagaatgtca aactttgcct tcaactgttt ttatttctag tctcttccac
     4261 tttaaaacaa aatgaacact gcttgtcttc ttccattgac catttagtgt tgagtactgt
     4321 atgtgttttg ttaattctat aaaggtatct gttagatatt aaaggtgaga attagggcag
     4381 gttaatcaaa aatggggaag gggaaatggt aaccaaaaag taaccccatg gtaaggttta
     4441 tatgagtata tgtgaatata gagctaggaa aaaaagcccc cccaaatacc tttttaaccc
     4501 ctctgattgg ctattattac tatatttatt attatttatt gaaaccttag ggaagattga
     4561 agattcatcc catacttcta tataccatgc ttaaaaatca cgtcattctt taaacaaaaa
     4621 tactcaagat catttatatt tatttggaga gaaaactgtc ctaatttaga atttccctca
     4681 aatctgaggg acttttaaga aatgctaaca gatttttctg gaggaaattt agacaaaaca
     4741 atgtcattta gtagaatatt tcagtattta agtggaattt cagtatactg tactatcctt
     4801 tataagtcat taaaataatg tttcatcaaa tggttaaatg gaccactggt ttcttagaga
     4861 aatgttttta ggcttaattc attcaattgt caagtacact tagtcttaat acactcaggt
     4921 ttgaacagat tattctgaat attaaaattt aatccattct taatatttta aaacttttgt
     4981 taagaaaaac tgccagtttg tgcttttgaa atgtctgttt tgacatcata gtctagtaaa
     5041 attttgacag tgcatatgta ctgttactaa aagctttata tgaaattatt aatgtgaagt
     5101 ttttcattta taattcaagg aaggatttcc tgaaaacatt tcaagggatt tatgtctaca
     5161 tatttgtgtg tgtgtgtgta tatatatgta atatgcatac acagatgcat atgtgtatat
     5221 ataatgaaat ttatgttgct ggtattttgc attttaaagt gatcaagatt cattaggcaa
     5281 actttggttt aagtaaacat atgttcaaaa tcagattaac agatacaggt ttcatagaga
     5341 acaaaggtga tcatttgaag ggcatgctgt aatttcacac aattttccag ttcaaaaatg
     5401 gagaatactt cgcctaaaat actgttaagt gggttaattg atacaagttt ctgtggtgga
     5461 aaatttatgc aggttttcac gaatcctttt tttttttttt tttttttttt gagacggagt
     5521 cttgctctgt tgccacgctg gaatgcagta acgtgatctt ggctcactgc gacctccacc
     5581 tccccagttc aagcgattct cctgcctcag cctccctagt agctgggact acgggtgcac
     5641 gccaccatgc ccagctaatt tttgtatttt gagtagagac agggtttcac cgtgttggct
     5701 aggatggtgt ctatctcttg accttgtgat ccacccgcct cagcctccca gagtgctggg
     5761 attacaggtg cgagccactg cgcctggctg gttttcatga atcttgatag acatctataa
     5821 cgttattatt ttcagtggtg tgcagcattt ttgcttcatg agtatgacct aggtatagag
     5881 atctgataac ttgaattcag aatattaaga aaatgaagta actgattttc taaaaaaaaa
     5941 aaaaaaaaaa atttctacat tataactcac agcattgttc cattgcaggt tttgcaatgt
     6001 ttgggggtaa agacagtaga aatattattc agtaaacaat aatgtgtgaa cttttaagat
     6061 ggataatagg gcatggactg agtgctgcta tcttgaaatg tgcacaggta cacttacctt
     6121 tttttttttt ttttttaagt ttttcccatt caggaaaaca acattgtgat ctgtactaca
     6181 ggaaccaaat gtcatgcgtc atacatgtgg gtataaagta cataaaatat atctaactat
     6241 tcataatgtg gggtgggtaa tactgtctgt gaaataatgt aagaagcttt tcacttaaaa
     6301 aaaatgcatt actttcactt aacactagac accaggtcga aaattttcaa ggttatagta
     6361 cttatttcaa caattcttag agatgctagc tagtgttgaa gctaaaaata gctttattta
     6421 tgctgaattg tgattttttt atgccaaatt ttttttagtt ctaatcattg atgatagctt
     6481 ggaaataaat aattatgcca tggcatttga cagttcatta ttcctataag aattaaattg
     6541 agtttagaga gaatggtggt gttgagctga ttattaacag ttactgaaat caaatattta
     6601 tttgttacat tattccattt gtattttagg tttcctttta cattcttttt atatgcattc
     6661 tgacattaca tattttttaa gactatggaa ataatttaaa gatttaagct ctggtggatg
     6721 attatctgct aagtaagtct gaaaatgtaa tattttgata atactgtaat atacctgtca
     6781 cacaaatgct tttctaatgt tttaaccttg agtattgcag ttgctgcttt gtacagaggt
     6841 tactgcaata aaggaagtgg attcattaaa ccta
//
