LOCUS       NM_000338               4708 bp    mRNA    linear   PRI 20-OCT-2024
DEFINITION  Homo sapiens solute carrier family 12 member 1 (SLC12A1),
            transcript variant 1, mRNA.
ACCESSION   NM_000338
VERSION     NM_000338.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Frachon,N., Demaretz,S., Seaayfan,E., Chelbi,L., Bakhos-Douaihy,D.
            and Laghmani,K.
  TITLE     AUP1 Regulates the Endoplasmic Reticulum-Associated Degradation and
            Polyubiquitination of NKCC2
  JOURNAL   Cells 13 (5), 389 (2024)
   PUBMED   38474353
  REMARK    GeneRIF: AUP1 Regulates the Endoplasmic Reticulum-Associated
            Degradation and Polyubiquitination of NKCC2.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4708)
  AUTHORS   Gamba,G.
  TITLE     From Fish Physiology to Human Disease: The Discovery of the NCC,
            NKCC2, and the Cation-Coupled Chloride Cotransporters
  JOURNAL   Kidney360 5 (1), 133-141 (2024)
   PUBMED   37968800
  REMARK    GeneRIF: From Fish Physiology to Human Disease: The Discovery of
            the NCC, NKCC2, and the Cation-Coupled Chloride Cotransporters.
REFERENCE   3  (bases 1 to 4708)
  AUTHORS   Justo-Garrido,M., Lopez-Saavedra,A., Alcaraz,N.,
            Cortes-Gonzalez,C.C., Onate-Ocana,L.F., Caro-Sanchez,C.H.S.,
            Castro-Hernandez,C., Arriaga-Canon,C., Diaz-Chavez,J. and
            Herrera,L.A.
  TITLE     Association of SLC12A1 and GLUR4 Ion Transporters with Neoadjuvant
            Chemoresistance in Luminal Locally Advanced Breast Cancer
  JOURNAL   Int J Mol Sci 24 (22), 16104 (2023)
   PUBMED   38003293
  REMARK    GeneRIF: Association of SLC12A1 and GLUR4 Ion Transporters with
            Neoadjuvant Chemoresistance in Luminal Locally Advanced Breast
            Cancer.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4708)
  AUTHORS   Lai,X., Lu,S., Jiang,J., Zhang,H., Yang,Q., Liu,Y., Li,L., Li,S.,
            Dai,S., Chen,Y., Chen,Y., Liu,J. and Li,Y.
  TITLE     Association of polymorphisms of calcium reabsorption genes SLC12A1,
            KCNJ1 and SLC8A1 with colorectal adenoma
  JOURNAL   J Cancer Res Clin Oncol 149 (11), 8335-8344 (2023)
   PUBMED   37074453
  REMARK    GeneRIF: Association of polymorphisms of calcium reabsorption genes
            SLC12A1, KCNJ1 and SLC8A1 with colorectal adenoma.
REFERENCE   5  (bases 1 to 4708)
  AUTHORS   Castrop,H. and Schnermann,J.
  TITLE     Isoforms of renal Na-K-2Cl cotransporter NKCC2: expression and
            functional significance
  JOURNAL   Am J Physiol Renal Physiol 295 (4), F859-F866 (2008)
   PUBMED   18495801
  REMARK    Review article
REFERENCE   6  (bases 1 to 4708)
  AUTHORS   Vargas-Poussou,R., Feldmann,D., Vollmer,M., Konrad,M., Kelly,L.,
            van den Heuvel,L.P., Tebourbi,L., Brandis,M., Karolyi,L.,
            Hebert,S.C., Lemmink,H.H., Deschenes,G., Hildebrandt,F.,
            Seyberth,H.W., Guay-Woodford,L.M., Knoers,N.V. and Antignac,C.
  TITLE     Novel molecular variants of the Na-K-2Cl cotransporter gene are
            responsible for antenatal Bartter syndrome
  JOURNAL   Am J Hum Genet 62 (6), 1332-1340 (1998)
   PUBMED   9585600
REFERENCE   7  (bases 1 to 4708)
  AUTHORS   Kurtz,C.L., Karolyi,L., Seyberth,H.W., Koch,M.C., Vargas,R.,
            Feldmann,D., Vollmer,M., Knoers,N.V., Madrigal,G. and
            Guay-Woodford,L.M.
  TITLE     A common NKCC2 mutation in Costa Rican Bartter's syndrome patients:
            evidence for a founder effect
  JOURNAL   J Am Soc Nephrol 8 (11), 1706-1711 (1997)
   PUBMED   9355073
REFERENCE   8  (bases 1 to 4708)
  AUTHORS   Simon,D.B., Karet,F.E., Hamdan,J.M., DiPietro,A., Sanjad,S.A. and
            Lifton,R.P.
  TITLE     Bartter's syndrome, hypokalaemic alkalosis with hypercalciuria, is
            caused by mutations in the Na-K-2Cl cotransporter NKCC2
  JOURNAL   Nat Genet 13 (2), 183-188 (1996)
   PUBMED   8640224
REFERENCE   9  (bases 1 to 4708)
  AUTHORS   Quaggin,S.E., Payne,J.A., Forbush,B. 3rd and Igarashi,P.
  TITLE     Localization of the renal Na-K-Cl cotransporter gene (Slc12a1) on
            mouse chromosome 2
  JOURNAL   Mamm Genome 6 (8), 557-558 (1995)
   PUBMED   8589530
REFERENCE   10 (bases 1 to 4708)
  AUTHORS   Delpire,E., Rauchman,M.I., Beier,D.R., Hebert,S.C. and Gullans,S.R.
  TITLE     Molecular cloning and chromosome localization of a putative
            basolateral Na(+)-K(+)-2Cl- cotransporter from mouse inner
            medullary collecting duct (mIMCD-3) cells
  JOURNAL   J Biol Chem 269 (41), 25677-25683 (1994)
   PUBMED   7929272
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA632985.1, BC040138.2,
            U58130.1, AC023355.5 and AA917702.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jul 25, 2019 this sequence version replaced NM_000338.2.
            
            Summary: This gene encodes a kidney-specific
            sodium-potassium-chloride cotransporter that is expressed on the
            luminal membrane of renal epithelial cells of the thick ascending
            limb of Henle's loop and the macula densa. It plays a key role in
            concentrating urine and accounts for most of the NaCl resorption.
            It is sensitive to such diuretics as furosemide and bumetanide.
            Some Bartter-like syndromes result from defects in this gene.
            Alternative splicing results in multiple transcript variants
            encoding distinct isoforms. Additional splice variants have been
            described but their biological validity in humans has not been
            experimentally proven.[provided by RefSeq, May 2010].
            
            Transcript Variant: This variant (1) encodes isoform A. Isoforms A
            and F are the same length but differ in the region of amino acids
            214-238.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            CDS exon combination :: EF559316.2 [ECO:0000331]
            RNAseq introns       :: single sample supports all introns
                                    SAMEA1970526, SAMEA2145774 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000380993.8/ ENSP00000370381.3
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-571               DA632985.1         2-572
            572-1430            BC040138.2         543-1401
            1431-3087           U58130.1           1235-2891
            3088-3088           AC023355.5         71675-71675
            3089-3558           U58130.1           2893-3362
            3559-4599           AC023355.5         86088-87128
            4600-4708           AA917702.1         9-117               c
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q21.1"
     gene            1..4708
                     /gene="SLC12A1"
                     /gene_synonym="BSC; BSC-1; BSC1; CCC2; NKCC2"
                     /note="solute carrier family 12 member 1"
                     /db_xref="GeneID:6557"
                     /db_xref="HGNC:HGNC:10910"
                     /db_xref="MIM:600839"
     exon            1..29
                     /gene="SLC12A1"
                     /gene_synonym="BSC; BSC-1; BSC1; CCC2; NKCC2"
                     /inference="alignment:Splign:2.1.0"
     exon            30..635
                     /gene="SLC12A1"
                     /gene_synonym="BSC; BSC-1; BSC1; CCC2; NKCC2"
                     /inference="alignment:Splign:2.1.0"
     CDS             216..3515
                     /gene="SLC12A1"
                     /gene_synonym="BSC; BSC-1; BSC1; CCC2; NKCC2"
                     /note="isoform A is encoded by transcript variant 1;
                     Na-K-2Cl cotransporter; Na-K-2Cl cotransporter 2;
                     kidney-specific Na-K-Cl symporter; bumetanide-sensitive
                     sodium-(potassium)-chloride cotransporter 2; solute
                     carrier family 12 (sodium/potassium/chloride
                     transporters), member 1; solute carrier family 12
                     (sodium/potassium/chloride transporter), member 1;
                     bumetanide-sensitive cotransporter type 1;
                     bumetanide-sensitive sodium-(potassium)-chloride
                     cotransporter 1"
                     /codon_start=1
                     /product="solute carrier family 12 member 1 isoform A"
                     /protein_id="NP_000329.2"
                     /db_xref="CCDS:CCDS10129.2"
                     /db_xref="GeneID:6557"
                     /db_xref="HGNC:HGNC:10910"
                     /db_xref="MIM:600839"
                     /translation="MSLNNSSNVFLDSVPSNTNRFQVSVINENHESSAAADDNTDPPH
                     YEETSFGDEAQKRLRISFRPGNQECYDNFLQSGETAKTDASFHAYDSHTNTYYLQTFG
                     HNTMDAVPKIEYYRNTGSISGPKVNRPSLLEIHEQLAKNVAVTPSSADRVANGDGIPG
                     DEQAENKEDDQAGVVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVLIILL
                     STMVTSITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYV
                     VGFAETVVDLLKESDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLVIL
                     LIAIANFFIGTVIPSNNEKKSRGFFNYQASIFAENFGPRFTKGEGFFSVFAIFFPAAT
                     GILAGANISGDLEDPQDAIPRGTMLAIFITTVAYLGVAICVGACVVRDATGNMNDTII
                     SGMNCNGSAACGLGYDFSRCRHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSA
                     LASLVSAPKVFQALCKDNIYKALQFFAKGYGKNNEPLRGYILTFLIAMAFILIAELNT
                     IAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAVLCCAVMFVIN
                     WWAAVITYVIEFFLYVYVTCKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRP
                     QCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLI
                     KNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLTEIENYVG
                     IIHDAFDFEIGVVIVRISQGFDISQVLQVQEELERLEQERLALEATIKDNECEEESGG
                     IRGLFKKAGKLNITKTTPKKDGSINTSQSMHVGEFNQKLVEASTQFKKKQEKGTIDVW
                     WLFDDGGLTLLIPYILTLRKKWKDCKLRIYVGGKINRIEEEKIVMASLLSKFRIKFAD
                     IHIIGDINIRPNKESWKVFEEMIEPYRLHESCKDLTTAEKLKRETPWKITDAELEAVK
                     EKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNLPPVLLVR
                     GNHKNVLTFYS"
     exon            636..767
                     /gene="SLC12A1"
                     /gene_synonym="BSC; BSC-1; BSC1; CCC2; NKCC2"
                     /inference="alignment:Splign:2.1.0"
     exon            768..843
                     /gene="SLC12A1"
                     /gene_synonym="BSC; BSC-1; BSC1; CCC2; NKCC2"
                     /inference="alignment:Splign:2.1.0"
     exon            844..939
                     /gene="SLC12A1"
                     /gene_synonym="BSC; BSC-1; BSC1; CCC2; NKCC2"
                     /inference="alignment:Splign:2.1.0"
     exon            940..1079
                     /gene="SLC12A1"
                     /gene_synonym="BSC; BSC-1; BSC1; CCC2; NKCC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1080..1190
                     /gene="SLC12A1"
                     /gene_synonym="BSC; BSC-1; BSC1; CCC2; NKCC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1191..1302
                     /gene="SLC12A1"
                     /gene_synonym="BSC; BSC-1; BSC1; CCC2; NKCC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1303..1430
                     /gene="SLC12A1"
                     /gene_synonym="BSC; BSC-1; BSC1; CCC2; NKCC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1431..1515
                     /gene="SLC12A1"
                     /gene_synonym="BSC; BSC-1; BSC1; CCC2; NKCC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1516..1667
                     /gene="SLC12A1"
                     /gene_synonym="BSC; BSC-1; BSC1; CCC2; NKCC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1668..1775
                     /gene="SLC12A1"
                     /gene_synonym="BSC; BSC-1; BSC1; CCC2; NKCC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1776..1899
                     /gene="SLC12A1"
                     /gene_synonym="BSC; BSC-1; BSC1; CCC2; NKCC2"
                     /inference="alignment:Splign:2.1.0"
     exon            1900..2001
                     /gene="SLC12A1"
                     /gene_synonym="BSC; BSC-1; BSC1; CCC2; NKCC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2002..2157
                     /gene="SLC12A1"
                     /gene_synonym="BSC; BSC-1; BSC1; CCC2; NKCC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2158..2257
                     /gene="SLC12A1"
                     /gene_synonym="BSC; BSC-1; BSC1; CCC2; NKCC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2258..2369
                     /gene="SLC12A1"
                     /gene_synonym="BSC; BSC-1; BSC1; CCC2; NKCC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2370..2510
                     /gene="SLC12A1"
                     /gene_synonym="BSC; BSC-1; BSC1; CCC2; NKCC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2511..2617
                     /gene="SLC12A1"
                     /gene_synonym="BSC; BSC-1; BSC1; CCC2; NKCC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2618..2700
                     /gene="SLC12A1"
                     /gene_synonym="BSC; BSC-1; BSC1; CCC2; NKCC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2701..2844
                     /gene="SLC12A1"
                     /gene_synonym="BSC; BSC-1; BSC1; CCC2; NKCC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2845..2976
                     /gene="SLC12A1"
                     /gene_synonym="BSC; BSC-1; BSC1; CCC2; NKCC2"
                     /inference="alignment:Splign:2.1.0"
     exon            2977..3088
                     /gene="SLC12A1"
                     /gene_synonym="BSC; BSC-1; BSC1; CCC2; NKCC2"
                     /inference="alignment:Splign:2.1.0"
     exon            3089..3175
                     /gene="SLC12A1"
                     /gene_synonym="BSC; BSC-1; BSC1; CCC2; NKCC2"
                     /inference="alignment:Splign:2.1.0"
     exon            3176..3311
                     /gene="SLC12A1"
                     /gene_synonym="BSC; BSC-1; BSC1; CCC2; NKCC2"
                     /inference="alignment:Splign:2.1.0"
     exon            3312..3379
                     /gene="SLC12A1"
                     /gene_synonym="BSC; BSC-1; BSC1; CCC2; NKCC2"
                     /inference="alignment:Splign:2.1.0"
     exon            3380..4708
                     /gene="SLC12A1"
                     /gene_synonym="BSC; BSC-1; BSC1; CCC2; NKCC2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4688..4693
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLC12A1"
                     /gene_synonym="BSC; BSC-1; BSC1; CCC2; NKCC2"
                     /note="hexamer: AATAAA"
     polyA_site      4708
                     /gene="SLC12A1"
                     /gene_synonym="BSC; BSC-1; BSC1; CCC2; NKCC2"
                     /note="major polyA site"
ORIGIN      
        1 acatttcctc agaagaaggc tccttggtgc tttgaagaac atcctgaaga ttatatcgga
       61 gacaatatat caagaatcta tttattgaat catctagaac aaaagccagg agctccctaa
      121 tggaagcaca ttagtgttta ttttgatgaa gaaatatata gattttttaa aacaaccaca
      181 aagtagatag ctcagtaaaa aatcaatttt ggaagatgtc actgaacaac tcttccaatg
      241 tatttctgga ttcagtgccc agtaatacca atcgctttca agttagtgtc ataaatgaga
      301 accatgagag cagtgcagct gcagatgaca atactgaccc accacattat gaagaaacct
      361 cttttgggga tgaagctcag aaaagactca gaatcagctt taggcctggg aatcaggagt
      421 gctatgacaa tttcctccaa agtggagaaa ctgctaaaac agatgccagt tttcacgctt
      481 atgattctca cacaaacaca tactatctac aaacttttgg ccacaacacc atggatgccg
      541 ttcccaagat agagtactat cgtaacaccg gcagcatcag tgggcccaag gtcaaccgac
      601 ccagcctgct tgagattcac gagcaactcg caaagaatgt ggcagtcacc ccaagttcag
      661 ctgacagagt tgctaacggt gatgggatac ctggagatga acaagctgaa aataaggaag
      721 atgatcaagc tggtgttgtg aagtttggat gggtgaaagg tgtgctggta agatgcatgc
      781 tgaacatctg gggagtcatg ctcttcattc gcctctcctg gattgttgga gaagctggaa
      841 ttggtcttgg agttctcata attcttcttt ccaccatggt aacttctatt actgggttgt
      901 caacttctgc gatagcaact aacgggtttg ttcgtggagg tggggcctac tatcttattt
      961 ccagaagttt agggcccgag ttcggtgggt caataggcct gatctttgct tttgctaatg
     1021 cagtggctgt tgctatgtat gtggtgggat ttgctgagac tgtagtagat cttcttaagg
     1081 agagtgattc gatgatggtg gatccaacca atgacatccg gattataggc tccatcacag
     1141 tggtgattct tctaggaatt tcagtagctg gaatggaatg ggaggcaaag gcccaagtca
     1201 ttcttctggt cattcttcta attgctattg caaacttctt cattggaact gtcattccat
     1261 ccaacaatga gaaaaagtcc agaggtttct ttaattacca agcatcaata tttgcagaaa
     1321 actttgggcc acgcttcaca aagggtgaag gcttcttctc tgtctttgcc atttttttcc
     1381 cagcagctac tgggattctt gctggtgcca atatctcagg agatttggag gatccccaag
     1441 atgccatccc cagaggaacc atgctggcca ttttcatcac cactgttgcc tacttagggg
     1501 ttgcaatttg tgtaggggcc tgtgtggtcc gagatgccac cgggaacatg aatgacacca
     1561 tcatttctgg gatgaactgc aatggttcag cagcatgtgg gttgggctat gacttctcaa
     1621 gatgtcgaca tgaaccatgt cagtacgggc tgatgaacaa tttccaggtc atgagcatgg
     1681 tatcagggtt cggccccctc atcactgcgg gaatcttttc tgcaacactc tcctccgccc
     1741 tggcctccct tgtcagcgca cccaaagtgt tccaggctct gtgcaaggac aacatctaca
     1801 aagccctgca gttttttgca aagggatatg ggaaaaacaa tgaacccctg agaggatata
     1861 ttctcacttt tcttatagcc atggcattta ttcttattgc ggaactgaac accattgctc
     1921 ccatcatctc caactttttc ctggcctcat atgcacttat taatttctcc tgcttccatg
     1981 cctcttatgc caaatctcca ggatggagac ctgcgtatgg aatttacaac atgtgggtat
     2041 ctctttttgg agctgttttg tgctgtgcag tcatgtttgt catcaactgg tgggcagctg
     2101 tcatcaccta tgtcattgaa ttcttccttt acgtctatgt gacttgtaag aagccagatg
     2161 tgaactgggg ctcctccaca caggctcttt cctacgtgag tgctttagac aatgctctgg
     2221 aattaaccac agtggaagac cacgtaaaaa acttcaggcc ccagtgcatt gtcttaacag
     2281 ggggacccat gacaagacct gctctcctgg acataactca cgcctttacc aagaacagtg
     2341 gcctttgcat ctgctgtgaa gtctttgtgg gaccgcgcaa actgtgtgtt aaggagatga
     2401 acagtggcat ggcgaaaaaa caggcctggc ttataaagaa caaaatcaag gctttttatg
     2461 ctgcagtggc ggcagactgt ttcagggatg gtgtccgaag tcttcttcag gcctcaggct
     2521 taggaagaat gaaaccaaac actctggtga ttggatataa gaaaaactgg aggaaagctc
     2581 ccttgacaga gattgagaac tacgtgggaa tcatacatga tgcatttgat tttgagattg
     2641 gcgtggttat agtcagaatc agccaaggat ttgacatctc tcaggttctt caggtgcaag
     2701 aggaattaga gagattagaa caggagagac tagcattgga agcgactatc aaagataatg
     2761 agtgtgaaga ggaaagtgga ggcatccgag gcttgtttaa aaaagctggc aagttgaaca
     2821 ttactaagac aacgcctaaa aaagatggca gcattaacac aagccagtcg atgcatgtgg
     2881 gagagttcaa ccagaaactg gtggaagcca gcactcaatt taaaaagaaa caagaaaaag
     2941 gcacaattga tgtttggtgg ttgtttgatg atggagggtt aacacttctt atcccctata
     3001 tcttaactct cagaaaaaaa tggaaagact gtaaattaag aatctatgtg ggagggaaga
     3061 tcaaccgcat tgaagaagaa aaaattgtaa tggcttccct tctgagcaaa tttaggataa
     3121 aatttgcaga catccatatc atcggtgaca tcaacattag gccaaacaaa gagagctgga
     3181 aagtctttga agagatgatt gaaccatatc gtctccatga aagctgcaaa gatttaacaa
     3241 ctgctgagaa attaaaaaga gaaactccgt ggaaaattac agatgcagaa ctggaagcag
     3301 tcaaggaaaa gagttaccgc caagttcgac tgaatgaact cttacaggag cactccagag
     3361 ctgctaatct cattgtcctg agccttcccg tggcaagaaa gggatccata tcggatttgt
     3421 tgtatatggc ttggttggaa atcctcacaa agaacctccc acctgtctta ctagttagag
     3481 gaaatcacaa aaatgtcttg acattttact cttaaaacat gaaagattgg aatacatttt
     3541 aacttaatgt aatgcataat taagaaacat gttccagtac tttatgttgt aaatctgatc
     3601 tatggatatg caaacctctg gagaggatcc taccagattc tacatacatt gcataatttt
     3661 tatcagttaa tgcgagcttt tttttctctt ctcagcttaa ggggttgtca aagccaatgt
     3721 tatccctaga aaaacatttt tgtcactgct gttgataaac aagaaaatca aggaaactca
     3781 tgttggctta tgctcatgaa aaccaccaat gtgattgtaa acttctccag acaaacttaa
     3841 ccttttgttt cttaattttt tgttttgagt gtttgcttct cagccctggt ataggtctca
     3901 gccccacagc aggatctcaa taaatgctta ttgacaggct ggcatagtaa ccacaggtgg
     3961 ttatcaaagg aaaagaccaa taggctgggg aacataatta tgcttggccc cctcagaggc
     4021 ttatacctcc aaagcagatt taaaaatcag taactcaaaa ctttaagaag tagttgagtc
     4081 tacaaaatat tcaaagcagt tacctaaata aggttattta atgtaacaga ttatatactt
     4141 aaagtgatct gagcaatcat tgataacata tggcttaaat ttgctgctgc ctgaaaagta
     4201 tattaactag attagaagac accatatact ccaataaaat gaaatatatt ctattctcaa
     4261 tatataaact ttaattgtac tccctatcaa atattttgaa atttccatca cggcattact
     4321 ttgcatattt ttttctaatt aaatggttag tacagatgaa atctttatag atgactactt
     4381 gaaaactaag tgctacaaat tttcaaacca ttattaagaa atatatcctg aatcatatat
     4441 ttagttactt tctatagtga ttgtatggat ttaaaagaat aattaaaatg tgaagttgaa
     4501 aaacttgtaa aatttggaat atacagatac tgattttttt catttttgtt agtataccta
     4561 taatacataa tcacattgac aaaccaatga aaatattgat tttctgatga atggcttgat
     4621 ttttctcctt gacatgtttt gactgttcat tctaactgaa aacatatatg ggaaaatatg
     4681 aacctgaaat aaaagcactc aaattcaa
//
