LOCUS       NM_032214               2737 bp    mRNA    linear   PRI 05-APR-2024
DEFINITION  Homo sapiens Src like adaptor 2 (SLA2), transcript variant 1, mRNA.
ACCESSION   NM_032214
VERSION     NM_032214.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Wybenga-Groot,L.E., Tench,A.J., Simpson,C.D., Germain,J.S.,
            Raught,B., Moran,M.F. and McGlade,C.J.
  TITLE     SLAP2 Adaptor Binding Disrupts c-CBL Autoinhibition to Activate
            Ubiquitin Ligase Function
  JOURNAL   J Mol Biol 433 (8), 166880 (2021)
   PUBMED   33617900
  REMARK    GeneRIF: SLAP2 Adaptor Binding Disrupts c-CBL Autoinhibition to
            Activate Ubiquitin Ligase Function.
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 2737)
  AUTHORS   Marton,N., Baricza,E., Ersek,B., Buzas,E.I. and Nagy,G.
  TITLE     The Emerging and Diverse Roles of Src-Like Adaptor Proteins in
            Health and Disease
  JOURNAL   Mediators Inflamm 2015, 952536 (2015)
   PUBMED   26339145
  REMARK    Review article
REFERENCE   4  (bases 1 to 2737)
  AUTHORS   Leung,K.K., Hause,R.J. Jr., Barkinge,J.L., Ciaccio,M.F., Chuu,C.P.
            and Jones,R.B.
  TITLE     Enhanced prediction of Src homology 2 (SH2) domain binding
            potentials using a fluorescence polarization-derived c-Met, c-Kit,
            ErbB, and androgen receptor interactome
  JOURNAL   Mol Cell Proteomics 13 (7), 1705-1723 (2014)
   PUBMED   24728074
REFERENCE   5  (bases 1 to 2737)
  AUTHORS   Sugihara,S., Katsutani,S., Deckmyn,H., Fujimura,K. and Kimura,A.
  TITLE     Roles of Src-like adaptor protein 2 (SLAP-2) in GPVI-mediated
            platelet activation SLAP-2 and GPVI signaling
  JOURNAL   Thromb Res 126 (4), e276-e285 (2010)
   PUBMED   20828795
  REMARK    GeneRIF: In platelets, SLAP-2 may have function as a negative
            regulator of GPVI-mediated signaling by interacting with c-Cbl.
REFERENCE   6  (bases 1 to 2737)
  AUTHORS   Jones,R.B., Gordus,A., Krall,J.A. and MacBeath,G.
  TITLE     A quantitative protein interaction network for the ErbB receptors
            using protein microarrays
  JOURNAL   Nature 439 (7073), 168-174 (2006)
   PUBMED   16273093
REFERENCE   7  (bases 1 to 2737)
  AUTHORS   Loreto,M.P. and McGlade,C.J.
  TITLE     Cloning and characterization of human Src-like adaptor protein 2
            and a novel splice isoform, SLAP-2-v
  JOURNAL   Oncogene 22 (2), 266-273 (2003)
   PUBMED   12527895
  REMARK    GeneRIF: The human SLAP-2 gene is located on chromosome 20q, and
            the SLAP-2 coding region consists of seven exons, a unique cDNA
            encoding an alternatively spliced SLAP-2 isoform has been
            identified
REFERENCE   8  (bases 1 to 2737)
  AUTHORS   Loreto,M.P., Berry,D.M. and McGlade,C.J.
  TITLE     Functional cooperation between c-Cbl and Src-like adaptor protein 2
            in the negative regulation of T-cell receptor signaling
  JOURNAL   Mol Cell Biol 22 (12), 4241-4255 (2002)
   PUBMED   12024036
REFERENCE   9  (bases 1 to 2737)
  AUTHORS   Pandey,A., Ibarrola,N., Kratchmarova,I., Fernandez,M.M.,
            Constantinescu,S.N., Ohara,O., Sawasdikosol,S., Lodish,H.F. and
            Mann,M.
  TITLE     A novel Src homology 2 domain-containing molecule, Src-like adapter
            protein-2 (SLAP-2), which negatively regulates T cell receptor
            signaling
  JOURNAL   J Biol Chem 277 (21), 19131-19138 (2002)
   PUBMED   11891219
  REMARK    GeneRIF: negatively regulates T cell receptor signaling
REFERENCE   10 (bases 1 to 2737)
  AUTHORS   Holland,S.J., Liao,X.C., Mendenhall,M.K., Zhou,X., Pardo,J.,
            Chu,P., Spencer,C., Fu,A., Sheng,N., Yu,P., Pali,E., Nagin,A.,
            Shen,M., Yu,S., Chan,E., Wu,X., Li,C., Woisetschlager,M.,
            Aversa,G., Kolbinger,F., Bennett,M.K., Molineaux,S., Luo,Y.,
            Payan,D.G., Mancebo,H.S. and Wu,J.
  TITLE     Functional cloning of Src-like adapter protein-2 (SLAP-2), a novel
            inhibitor of antigen receptor signaling
  JOURNAL   J Exp Med 194 (9), 1263-1276 (2001)
   PUBMED   11696592
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA983348.1, BC042041.1,
            BF510664.1 and AL050318.13.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_032214.3.
            
            Summary: This gene encodes a member of the SLAP family of adapter
            proteins. The encoded protein may play an important
            receptor-proximal role in downregulating T and B cell-mediated
            responses and inhibits antigen receptor-induced calcium
            mobilization. This protein interacts with Cas-Br-M (murine)
            ecotropic retroviral transforming sequence c. Two transcript
            variants encoding distinct isoforms have been identified for this
            gene. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK291513.1, BC042041.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000262866.9/ ENSP00000262866.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-127               DA983348.1         21-147
            128-2525            BC042041.1         87-2484
            2526-2536           BF510664.1         14-24               c
            2537-2737           AL050318.13        123036-123236       c
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q11.23"
     gene            1..2737
                     /gene="SLA2"
                     /gene_synonym="C20orf156; MARS; SLAP-2; SLAP2"
                     /note="Src like adaptor 2"
                     /db_xref="GeneID:84174"
                     /db_xref="HGNC:HGNC:17329"
                     /db_xref="MIM:606577"
     exon            1..360
                     /gene="SLA2"
                     /gene_synonym="C20orf156; MARS; SLAP-2; SLAP2"
                     /inference="alignment:Splign:2.1.0"
     exon            361..494
                     /gene="SLA2"
                     /gene_synonym="C20orf156; MARS; SLAP-2; SLAP2"
                     /inference="alignment:Splign:2.1.0"
     CDS             404..1189
                     /gene="SLA2"
                     /gene_synonym="C20orf156; MARS; SLAP-2; SLAP2"
                     /note="isoform a is encoded by transcript variant 1;
                     Src-like adapter protein-2; modulator of antigen receptor
                     signaling"
                     /codon_start=1
                     /product="src-like-adapter 2 isoform a"
                     /protein_id="NP_115590.1"
                     /db_xref="CCDS:CCDS13282.1"
                     /db_xref="GeneID:84174"
                     /db_xref="HGNC:HGNC:17329"
                     /db_xref="MIM:606577"
                     /translation="MGSLPSRRKSLPSPSLSSSVQGQGPVTMEAERSKATAVALGSFP
                     AGGPAELSLRLGEPLTIVSEDGDWWTVLSEVSGREYNIPSVHVAKVSHGWLYEGLSRE
                     KAEELLLLPGNPGGAFLIRESQTRRGSYSLSVRLSRPASWDRIRHYRIHCLDNGWLYI
                     SPRLTFPSLQALVDHYSELADDICCLLKEPCVLQRAGPLPGKDIPLPVTVQRTPLNWK
                     ELDSSLLFSEAATGEESLLSEGLRESLSFYISLNDEAVSLDDA"
     exon            495..594
                     /gene="SLA2"
                     /gene_synonym="C20orf156; MARS; SLAP-2; SLAP2"
                     /inference="alignment:Splign:2.1.0"
     exon            595..681
                     /gene="SLA2"
                     /gene_synonym="C20orf156; MARS; SLAP-2; SLAP2"
                     /inference="alignment:Splign:2.1.0"
     exon            682..785
                     /gene="SLA2"
                     /gene_synonym="C20orf156; MARS; SLAP-2; SLAP2"
                     /inference="alignment:Splign:2.1.0"
     exon            786..935
                     /gene="SLA2"
                     /gene_synonym="C20orf156; MARS; SLAP-2; SLAP2"
                     /inference="alignment:Splign:2.1.0"
     exon            936..1068
                     /gene="SLA2"
                     /gene_synonym="C20orf156; MARS; SLAP-2; SLAP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1069..2737
                     /gene="SLA2"
                     /gene_synonym="C20orf156; MARS; SLAP-2; SLAP2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2504..2509
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLA2"
                     /gene_synonym="C20orf156; MARS; SLAP-2; SLAP2"
                     /note="hexamer: ATTAAA"
     polyA_site      2538
                     /gene="SLA2"
                     /gene_synonym="C20orf156; MARS; SLAP-2; SLAP2"
                     /note="major polyA site"
     polyA_site      2552
                     /gene="SLA2"
                     /gene_synonym="C20orf156; MARS; SLAP-2; SLAP2"
     regulatory      2712..2717
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLA2"
                     /gene_synonym="C20orf156; MARS; SLAP-2; SLAP2"
                     /note="hexamer: ATTAAA"
     polyA_site      2737
                     /gene="SLA2"
                     /gene_synonym="C20orf156; MARS; SLAP-2; SLAP2"
ORIGIN      
        1 agtcggagct agagctccaa ggaccccacg cctgtgtctc tgtgacagag ctcaaagggc
       61 cctgggcctt ccctccctgg ctcggctgtg cttgggaggg ttccccagtc cagaatccct
      121 aaggagcatg gggcagctga tccatccctg gtgtacaaac tgctgactgc agacagatgc
      181 tgagctaccc aaaccaacac ctagcctctc cctgaagatc ctcccaggct gagagagttc
      241 tgggtgtcct aggaccaagg acactggcag acttccagaa gggcccccaa agccctaacc
      301 tgtccagcca gagcatgcgt ctcagcagag ctgtcttccc aagcctttga tgacaaacca
      361 atttccctcg atgatgtgct tctgagtgct ctgctgagga acaatgggaa gtctgcccag
      421 cagaagaaaa tctctgccaa gcccaagctt gagttcctct gtccaaggcc agggacctgt
      481 gaccatggaa gcagagagaa gcaaggccac agccgtggcc ctgggcagtt tcccggcagg
      541 tggcccggcc gagctgtcgc tgagactcgg ggagccattg accatcgtct ctgaggatgg
      601 agactggtgg acggtgctgt ctgaagtctc aggcagagag tataacatcc ccagcgtcca
      661 cgtggccaaa gtctcccatg ggtggctgta tgagggcctg agcagggaga aagcagagga
      721 actgctgttg ttacctggga accctggagg ggccttcctc atccgggaga gccagaccag
      781 gagaggctct tactctctgt cagtccgcct cagccgccct gcatcctggg accggatcag
      841 acactacagg atccactgcc ttgacaatgg ctggctgtac atctcaccgc gcctcacctt
      901 cccctcactc caggccctgg tggaccatta ctctgagctg gcggatgaca tctgctgcct
      961 actcaaggag ccctgtgtcc tgcagagggc tggcccgctc cctggcaagg atatacccct
     1021 acctgtgact gtgcagagga caccactcaa ctggaaagag ctggacagct ccctcctgtt
     1081 ttctgaagct gccacagggg aggagtctct tctcagtgag ggtctccggg agtccctcag
     1141 cttctacatc agcctgaatg acgaggctgt ctctttggat gatgcctagg cccaaaggag
     1201 aggccaaaag ggaaaccaag gctgcacacc tagaacccca attcagcctc ctgggcaccc
     1261 cagaggcaag gctgtgcact cagggaggga gggtgggaca cagaggtgca tctagggtcc
     1321 cacctgtacc cttgctcttt cctctcttag cccttagaag tcacctactt ccttccagtg
     1381 ccatgatccc acctgcgacc tctagtgcga gtgcagagaa ggtgggacca gggccagggt
     1441 tccaaaaaga gaataagcct cctggggggt ctgacctagt tagttcttga gtttggggtt
     1501 tccagtacca tctggatgcc ctgcctgttg agccccattc tacatcccca ccattaacca
     1561 ggccccaccc acaaggtaga aacaacccct agagtcaacg agaaagtcat tttcagaaaa
     1621 tctacaagtc tcgttgagac caccaccata cctcagaagg taggactgtg gcctagaagg
     1681 gaaaggaaag ctgagatgat gtcttaccgt agcagcagat cttggatggt ccaggctcta
     1741 tgtgacctcc agagcaaaga gaaagacttc ggacagtcta ggtcctcaaa tgtcccccat
     1801 tgaggacaac agccccagct ctttttcttt tttttgagac ggagtcttgc cctgttgccc
     1861 atgctggagt gcaatggcac gatctcagct cactgcaacc tccatctcct ggattcaaac
     1921 aattctcctg cctcagcctc cagaatagct gggattacag gcgtacacca ccatgcctgg
     1981 ctaatttttt tgtattttta gtagacatgg ggtttcacca cattggccag gctggtgtcg
     2041 aactcctgac ctcaggtgat ccacccacct tggcctccca aagtgctggg attacaggtg
     2101 tgagccacgg cacccagcct agctctcaga tctctatttc attttgtggc ttaccattcc
     2161 ctagcacact ggccttgcca tcttgtggcc gaataaaaaa taacacctct taagtctagc
     2221 acactgcagt gaggccaggc acctcagtgc tgggcagggg catcagaagg tgctaagccc
     2281 tctctccaca atgccaagac ggagaccaca gcctacacca aatccagccc ttgatttccc
     2341 tgctgcctcc ataaacagaa agaggtctgc tggatccgct aagggatcag ggagaggaag
     2401 aaagagggat ggggtgggag gcaccccctc cagtgctcct actggttccc aagctacagg
     2461 tggggtggga aaggctttat caggtatcat caacaggttc tcaattaaag atttgattta
     2521 ttcaagtatg tgaaaacatt ctacaatgga aactcttatt agatgctgca tgtactgtgc
     2581 tatggaccac gcacatacag ccatgctgtt tcagaagact tgaaatgcca ttgatagttt
     2641 aaaaactcta cacccgatgg agaatcgagg aagacaattt aatgtttcat ctgaatccag
     2701 aggtgcatca aattaaatga cagctccact tggcaaa
//
