LOCUS       NM_001045556            2995 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens Src like adaptor (SLA), transcript variant 1, mRNA.
ACCESSION   NM_001045556
VERSION     NM_001045556.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 2995)
  AUTHORS   Kennedy,S.A., Jarboui,M.A., Srihari,S., Raso,C., Bryan,K.,
            Dernayka,L., Charitou,T., Bernal-Llinares,M., Herrera-Montavez,C.,
            Krstic,A., Matallanas,D., Kotlyar,M., Jurisica,I., Curak,J.,
            Wong,V., Stagljar,I., LeBihan,T., Imrie,L., Pillai,P., Lynn,M.A.,
            Fasterius,E., Al-Khalili Szigyarto,C., Breen,J., Kiel,C.,
            Serrano,L., Rauch,N., Rukhlenko,O., Kholodenko,B.N.,
            Iglesias-Martinez,L.F., Ryan,C.J., Pilkington,R., Cammareri,P.,
            Sansom,O., Shave,S., Auer,M., Horn,N., Klose,F., Ueffing,M.,
            Boldt,K., Lynn,D.J. and Kolch,W.
  TITLE     Extensive rewiring of the EGFR network in colorectal cancer cells
            expressing transforming levels of KRASG13D
  JOURNAL   Nat Commun 11 (1), 499 (2020)
   PUBMED   31980649
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2995)
  AUTHORS   Sharma,N., Ponce,M., Kaul,S., Pan,Z., Berry,D.M., Eiwegger,T. and
            McGlade,C.J.
  TITLE     SLAP Is a Negative Regulator of FcepsilonRI Receptor-Mediated
            Signaling and Allergic Response
  JOURNAL   Front Immunol 10, 1020 (2019)
   PUBMED   31156621
  REMARK    GeneRIF: SLAP Is a Negative Regulator of FcepsilonRI
            Receptor-Mediated Signaling and Allergic Response.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2995)
  AUTHORS   Tang,J., Sawasdikosol,S., Chang,J.H. and Burakoff,S.J.
  TITLE     SLAP, a dimeric adapter protein, plays a functional role in T cell
            receptor signaling
  JOURNAL   Proc Natl Acad Sci U S A 96 (17), 9775-9780 (1999)
   PUBMED   10449770
REFERENCE   6  (bases 1 to 2995)
  AUTHORS   Roche,S., Alonso,G., Kazlauskas,A., Dixit,V.M., Courtneidge,S.A.
            and Pandey,A.
  TITLE     Src-like adaptor protein (Slap) is a negative regulator of
            mitogenesis
  JOURNAL   Curr Biol 8 (17), 975-978 (1998)
   PUBMED   9742401
REFERENCE   7  (bases 1 to 2995)
  AUTHORS   Meijerink,P.H., Yanakiev,P., Zorn,I., Grierson,A.J., Bikker,H.,
            Dye,D., Kalaydjieva,L. and Baas,F.
  TITLE     The gene for the human Src-like adaptor protein (hSLAP) is located
            within the 64-kb intron of the thyroglobulin gene
  JOURNAL   Eur J Biochem 254 (2), 297-303 (1998)
   PUBMED   9660183
REFERENCE   8  (bases 1 to 2995)
  AUTHORS   Ohtsuki,T., Hatake,K., Ikeda,M., Tomizuka,H., Terui,Y., Uwai,M. and
            Miura,Y.
  TITLE     Expression of Src-like adapter protein mRNA is induced by all-trans
            retinoic acid
  JOURNAL   Biochem Biophys Res Commun 230 (1), 81-84 (1997)
   PUBMED   9020066
REFERENCE   9  (bases 1 to 2995)
  AUTHORS   Angrist,M., Wells,D.E., Chakravarti,A. and Pandey,A.
  TITLE     Chromosomal localization of the mouse Src-like adapter protein
            (Slap) gene and its putative human homolog SLA
  JOURNAL   Genomics 30 (3), 623-625 (1995)
   PUBMED   8825655
REFERENCE   10 (bases 1 to 2995)
  AUTHORS   Pandey,A., Duan,H. and Dixit,V.M.
  TITLE     Characterization of a novel Src-like adapter protein that
            associates with the Eck receptor tyrosine kinase
  JOURNAL   J Biol Chem 270 (33), 19201-19204 (1995)
   PUBMED   7543898
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            U44403.1, BX648382.1, DB265860.1 and D89077.1.
            
            On Nov 23, 2018 this sequence version replaced NM_001045556.2.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes isoform (a).
            
            CCDS Note: This CCDS represents a 276 aa protein that is encoded by
            NM_001045556.2, which is based on BX648382.1 and several locally
            supporting ESTs. However, it should be noted that NM_001045556.2
            contains an upstream translation start codon at nts 504-506, as
            used in the NM_001045557.2 variant which encodes a 293 aa protein.
            The use of this upstream start codon in NM_001045556.2 would result
            in only a 15 aa ORF with a stop codon in the second exon. The short
            length of this upstream ORF may allow escape from nonsense-mediated
            mRNA decay (NMD) and translation reinitiation at the downstream
            start codon (nts 833-835) to encode the represented 276 aa protein,
            or alternatively, the weak Kozak signal at the upstream start codon
            may enable leaky scanning by the translation machinery and
            subsequent use of the downstream start codon. The 276 aa protein is
            consistent with reports in the literature, including PMIDs:7543898,
            8825655 and 9660183. Variants encoding both the longer and shorter
            isoforms are also present in mouse (NM_001029841.3 and NM_009192.2,
            respectively).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BX648382.1, SRR1803612.267737.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000338087.10/ ENSP00000337548.5
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-50                U44403.1           230-279
            51-210              BX648382.1         98-257
            211-211             DB265860.1         58-58
            212-328             BX648382.1         259-375
            329-2070            U44403.1           280-2021
            2071-2084           D89077.1           1744-1757
            2085-2995           BX648382.1         2131-3041
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8q24.22"
     gene            1..2995
                     /gene="SLA"
                     /gene_synonym="SLA1; SLAP"
                     /note="Src like adaptor"
                     /db_xref="GeneID:6503"
                     /db_xref="HGNC:HGNC:10902"
                     /db_xref="MIM:601099"
     exon            1..50
                     /gene="SLA"
                     /gene_synonym="SLA1; SLAP"
                     /inference="alignment:Splign:2.1.0"
     exon            51..328
                     /gene="SLA"
                     /gene_synonym="SLA1; SLAP"
                     /inference="alignment:Splign:2.1.0"
     exon            329..429
                     /gene="SLA"
                     /gene_synonym="SLA1; SLAP"
                     /inference="alignment:Splign:2.1.0"
     CDS             369..1199
                     /gene="SLA"
                     /gene_synonym="SLA1; SLAP"
                     /note="isoform a is encoded by transcript variant 1;
                     hSLAP; SLAP-1; src-like-adapter protein 1"
                     /codon_start=1
                     /product="src-like-adapter isoform a"
                     /protein_id="NP_001039021.1"
                     /db_xref="CCDS:CCDS6370.1"
                     /db_xref="GeneID:6503"
                     /db_xref="HGNC:HGNC:10902"
                     /db_xref="MIM:601099"
                     /translation="MGNSMKSTPAPAERPLPNPEGLDSDFLAVLSDYPSPDISPPIFR
                     RGEKLRVISDEGGWWKAISLSTGRESYIPGICVARVYHGWLFEGLGRDKAEELLQLPD
                     TKVGSFMIRESETKKGFYSLSVRHRQVKHYRIFRLPNNWYYISPRLTFQCLEDLVNHY
                     SEVADGLCCVLTTPCLTQSTAAPAVRASSSPVTLRQKTVDWRRVSRLQEDPEGTENPL
                     GVDESLFSYGLRESIASYLSLTSEDNTSFDRKKKSISLMYGGSKRKSSFFSSPPYFED
                     "
     exon            430..529
                     /gene="SLA"
                     /gene_synonym="SLA1; SLAP"
                     /inference="alignment:Splign:2.1.0"
     exon            530..616
                     /gene="SLA"
                     /gene_synonym="SLA1; SLAP"
                     /inference="alignment:Splign:2.1.0"
     exon            617..720
                     /gene="SLA"
                     /gene_synonym="SLA1; SLAP"
                     /inference="alignment:Splign:2.1.0"
     exon            721..852
                     /gene="SLA"
                     /gene_synonym="SLA1; SLAP"
                     /inference="alignment:Splign:2.1.0"
     exon            853..985
                     /gene="SLA"
                     /gene_synonym="SLA1; SLAP"
                     /inference="alignment:Splign:2.1.0"
     exon            986..2995
                     /gene="SLA"
                     /gene_synonym="SLA1; SLAP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2974..2979
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SLA"
                     /gene_synonym="SLA1; SLAP"
                     /note="hexamer: AATAAA"
     polyA_site      2995
                     /gene="SLA"
                     /gene_synonym="SLA1; SLAP"
                     /note="major polyA site"
ORIGIN      
        1 atcatttgct gcgaatggag aacatctcag gcagccctga tgctccaccg tccttgcaga
       61 gagaagcttg ctcagagtgg ttactgaaaa caggaacctg cccttctgca agccctgtgc
      121 cttcctcgtt cctagcagag gaagtatgcg gccagtttag caactgcagt tcttctgtgt
      181 agcatgggct gcttctccac aaccgcgaga agcccggctg cctgtttatt ctggagcagc
      241 agcagttgac agacggagag gcagtgactc tgcacccagc aatccctctg cagacagctc
      301 ccacaggagt catgcccagt gtgtgaaggc tctgggcatc accagcggcc ccagggaaaa
      361 agaaagaaat gggaaacagc atgaaatcca cccctgcgcc tgccgagagg cccctgccca
      421 acccggaggg actggatagc gacttccttg ccgtgctaag tgactacccg tctcctgaca
      481 tcagcccccc gatattccgc cgaggggaga aactgcgtgt gatttctgat gaagggggct
      541 ggtggaaagc tatttctctt agcactggtc gagagagtta catccctgga atatgtgtgg
      601 ccagagttta ccatggctgg ctgtttgagg gcctgggcag agacaaggcc gaggagctgc
      661 tgcagctgcc agacacaaag gtcggctcct tcatgatcag agagagtgag accaagaaag
      721 ggttttactc actgtcggtg agacacaggc aggtaaagca ttaccgcatt ttccgtctgc
      781 ccaacaactg gtactacatt tccccgaggc tcaccttcca gtgcctggag gacctggtga
      841 accactattc tgaggtggct gatggcctgt gctgtgtgct caccacgccc tgcctgacac
      901 aaagcacggc tgccccagca gtgagggcct ccagctcacc tgtcaccttg cgtcagaaga
      961 ctgtggactg gaggagagtg tccagactgc aggaggaccc cgagggaaca gagaacccgc
     1021 ttggggtaga cgagtccctt ttcagctatg gccttcgaga gagcattgcc tcttacctgt
     1081 ccctgaccag tgaggacaac acctcctttg atcgaaagaa gaaaagcatc tccctgatgt
     1141 atggtggcag caagagaaag agctcattct tctcatcacc accttacttt gaggactagc
     1201 caagaacaga cacaatggtt catgcccaaa aggaacagaa gttccaacta ttgcctggga
     1261 tcttgcgaaa agcgaggttc cctgatccct gggagcctca cgtattttag aagccaagag
     1321 aagccacatg gagactcaaa ttcgcatctt ctctatccac atcatgacca aaggaacccc
     1381 tccctggtgt ctgatcaggg ctgtggcatc acgaaacatt ggatcatgac atgtcgggcg
     1441 atgcttggaa gagcccagca tgtatgtatg cacacattgt gtgtgtggga aggacaaagc
     1501 cactctcaca agaaagggca ccaggactgc tctccaagga actggacctg tccagacagt
     1561 tacactccaa ggtcattgga gagaacttct gtatgggcaa gcctgagagg gagaggaaac
     1621 aaaagctgtg tcctggcaga aggtctgggt ttgcagatgg gtgccctgaa tggaactact
     1681 ttaactaatc catagggact tctggtatgc tttcctctct ttttaaagga acttcgtgac
     1741 actaaacatt agcccaaagg acttcttagc cttcaattgg gagatacctt tggtctgctc
     1801 ctgcaccaaa gccatatggg tggaagtcag ttggcctccc tggttctgca gagggccaga
     1861 agaatgagag agaggaagac tgctggcagg gaaatcgagg aggcgagact agaactgcac
     1921 cagcttccct gatgtctgca gccatggctt tgcagcgcag acagagcttc tctgggatgc
     1981 tgggattctt gcctgtatga atgcatcaag tattcattta ttgcccgaat aggcattgca
     2041 ttaagtcctc tgtaaggtgt caggcaagcc aaaaaaaaaa aaaagatgcg taagtcctaa
     2101 cccccaacag aggtgttcac agtgtagaca gggaaaaaat gtataaacaa atgtgtaaaa
     2161 agagaaatca gctcatggct taggatggaa ttagagacag gtgagggaca ctcaggagct
     2221 cattttccag ctgctcttca gagtggaagg gctggctgga tcgggtaggt aagaatagct
     2281 ggatttttta gaaaagaaat ggatacagtc taaagaatta actcacccgg tactttattc
     2341 taagaagggt ctggcatcca tatgaggaaa aatgctcagc tccaggaaag atggggagtc
     2401 caagtggatt aatgatgtca tgcataattt taagagacaa gggagaaaac acaatgtata
     2461 gccagagaag gagaagctcc catccaaatc ctactaggaa gagagtgggc tgcagatgaa
     2521 tctgtgactc atgtttccct gtttcaaagg gatcctgggg aaggagggga acatgcttgc
     2581 agtatctctc cctgtctgtc tgctcacata agcattccgt ccatctgagc tcatcgtgct
     2641 actggtatgt gtatgtgcag ttacacagtt ttctgtatca tagattctag tgtgtttata
     2701 caaggagaca tctgtggttt ccccaaccgt tccaaaaggc tatttcaaag gaaccagcca
     2761 acgtatgaga aatgaatgta acactgtgga cattgacttc ccgcataagg cagggtgacc
     2821 ccctgaactc cagatgtctg cacagtatct tatgtgttgt tttccgttgt gacgaatgtg
     2881 attggaacat ttggggagca cccagaggga tttctcagtg ggaagcatta cactttgcta
     2941 aatcatgtat ttattcctga ttaaaacaaa cctaataaat atttaaccct tggca
//
