LOCUS       NM_005983               3715 bp    mRNA    linear   PRI 10-DEC-2024
DEFINITION  Homo sapiens S-phase kinase associated protein 2 (SKP2), transcript
            variant 1, mRNA.
ACCESSION   NM_005983
VERSION     NM_005983.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Chen,W., Chu,J., Miao,Y., Jiang,W., Wang,F., Zhang,N., Jin,J. and
            Cai,Y.
  TITLE     MOF-mediated acetylation of CDK9 promotes global transcription by
            modulating P-TEFb complex formation
  JOURNAL   FEBS J 291 (21), 4796-4812 (2024)
   PUBMED   39250546
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Matsumoto,N., Tajima,K., Takahashi,F., Mitsuishi,Y., Wirawan,A.,
            Hidayat,M., Winardi,W., Wibowo,A., Hayakawa,D., Izumi,K.,
            Kanamori,K., Miyashita,Y., Handa,T., Asao,T., Ko,R., Shukuya,T.,
            Shimada,N., Takamochi,K., Hayashi,T., Suzuki,K. and Takahashi,K.
  TITLE     Clinical potential of SKP2 as diagnostic marker and therapeutic
            target in small cell lung cancer
  JOURNAL   Respir Investig 62 (5), 901-909 (2024)
   PUBMED   39116798
  REMARK    GeneRIF: Clinical potential of SKP2 as diagnostic marker and
            therapeutic target in small cell lung cancer.
REFERENCE   3  (bases 1 to 3715)
  AUTHORS   Cigliano,A., Simile,M.M., Vidili,G., Pes,G.M., Dore,M.P., Urigo,F.,
            Cossu,E., Che,L., Feo,C., Steinmann,S.M., Ribback,S., Pascale,R.M.,
            Evert,M., Chen,X. and Calvisi,D.F.
  TITLE     Fatty Acid Synthase Promotes Hepatocellular Carcinoma Growth via
            S-Phase Kinase-Associated Protein 2/p27KIP1 Regulation
  JOURNAL   Medicina (Kaunas) 60 (7), 1160 (2024)
   PUBMED   39064589
  REMARK    GeneRIF: Fatty Acid Synthase Promotes Hepatocellular Carcinoma
            Growth via S-Phase Kinase-Associated Protein 2/p27[KIP1]
            Regulation.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3715)
  AUTHORS   Zhuang,M., Li,F., Liang,H., Su,Y., Cheng,L., Lin,B., Zhou,J.,
            Deng,R., Chen,L., Lyu,P. and Lu,Z.
  TITLE     Targeting RCC1 to block the human soft-tissue sarcoma by disrupting
            nucleo-cytoplasmic trafficking of Skp2
  JOURNAL   Cell Death Dis 15 (4), 241 (2024)
   PUBMED   38561375
  REMARK    GeneRIF: Targeting RCC1 to block the human soft-tissue sarcoma by
            disrupting nucleo-cytoplasmic trafficking of Skp2.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3715)
  AUTHORS   Feng,Z., Yin,J., Zhang,Z., Chen,Z., Huang,L., Tang,N. and Wang,K.
  TITLE     O-GlcNAcylation of E3 ubiquitin ligase SKP2 promotes hepatocellular
            carcinoma proliferation
  JOURNAL   Oncogene 43 (15), 1149-1159 (2024)
   PUBMED   38396292
  REMARK    GeneRIF: O-GlcNAcylation of E3 ubiquitin ligase SKP2 promotes
            hepatocellular carcinoma proliferation.
REFERENCE   6  (bases 1 to 3715)
  AUTHORS   Yam,C.H., Ng,R.W., Siu,W.Y., Lau,A.W. and Poon,R.Y.
  TITLE     Regulation of cyclin A-Cdk2 by SCF component Skp1 and F-box protein
            Skp2
  JOURNAL   Mol Cell Biol 19 (1), 635-645 (1999)
   PUBMED   9858587
REFERENCE   7  (bases 1 to 3715)
  AUTHORS   Ng,R.W., Arooz,T., Yam,C.H., Chan,I.W., Lau,A.W. and Poon,R.Y.
  TITLE     Characterization of the cullin and F-box protein partner Skp1
  JOURNAL   FEBS Lett 438 (3), 183-189 (1998)
   PUBMED   9827542
REFERENCE   8  (bases 1 to 3715)
  AUTHORS   Lisztwan,J., Marti,A., Sutterluty,H., Gstaiger,M., Wirbelauer,C.
            and Krek,W.
  TITLE     Association of human CUL-1 and ubiquitin-conjugating enzyme CDC34
            with the F-box protein p45(SKP2): evidence for evolutionary
            conservation in the subunit composition of the CDC34-SCF pathway
  JOURNAL   EMBO J 17 (2), 368-383 (1998)
   PUBMED   9430629
REFERENCE   9  (bases 1 to 3715)
  AUTHORS   Demetrick,D.J., Zhang,H. and Beach,D.H.
  TITLE     Chromosomal mapping of the genes for the human CDK2/cyclin
            A-associated proteins p19 (SKP1A and SKP1B) and p45 (SKP2)
  JOURNAL   Cytogenet Cell Genet 73 (1-2), 104-107 (1996)
   PUBMED   8646875
REFERENCE   10 (bases 1 to 3715)
  AUTHORS   Zhang,H., Kobayashi,R., Galaktionov,K. and Beach,D.
  TITLE     p19Skp1 and p45Skp2 are essential elements of the cyclin A-CDK2 S
            phase kinase
  JOURNAL   Cell 82 (6), 915-925 (1995)
   PUBMED   7553852
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA223617.1, AB050979.1,
            AK296223.1, AC008942.6 and CA415778.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_005983.3.
            
            Summary: This gene encodes a member of the F-box protein family
            which is characterized by an approximately 40 amino acid motif, the
            F-box. The F-box proteins constitute one of the four subunits of
            ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box),
            which function in phosphorylation-dependent ubiquitination. The
            F-box proteins are divided into 3 classes: Fbws containing WD-40
            domains, Fbls containing leucine-rich repeats, and Fbxs containing
            either different protein-protein interaction modules or no
            recognizable motifs. The protein encoded by this gene belongs to
            the Fbls class; in addition to an F-box, this protein contains 10
            tandem leucine-rich repeats. This protein is an essential element
            of the cyclin A-CDK2 S-phase kinase. It specifically recognizes
            phosphorylated cyclin-dependent kinase inhibitor 1B (CDKN1B, also
            referred to as p27 or KIP1) predominantly in S phase and interacts
            with S-phase kinase-associated protein 1 (SKP1 or p19). In
            addition, this gene is established as a protooncogene causally
            involved in the pathogenesis of lymphomas. Alternative splicing of
            this gene generates three transcript variants encoding different
            isoforms. [provided by RefSeq, Jul 2011].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660809.170022.1,
                                           SRR1660809.217904.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000274255.11/ ENSP00000274255.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-152               DA223617.1         69-220
            153-1427            AB050979.1         1-1275
            1428-1910           AK296223.1         1164-1646
            1911-3427           AC008942.6         47481-48997         c
            3428-3437           CA415778.1         16-25               c
            3438-3715           AC008942.6         47193-47470         c
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5p13.2"
     gene            1..3715
                     /gene="SKP2"
                     /gene_synonym="FBL1; FBXL1; FLB1; p45"
                     /note="S-phase kinase associated protein 2"
                     /db_xref="GeneID:6502"
                     /db_xref="HGNC:HGNC:10901"
                     /db_xref="MIM:601436"
     exon            1..160
                     /gene="SKP2"
                     /gene_synonym="FBL1; FBXL1; FLB1; p45"
                     /inference="alignment:Splign:2.1.0"
     CDS             153..1427
                     /gene="SKP2"
                     /gene_synonym="FBL1; FBXL1; FLB1; p45"
                     /note="isoform 1 is encoded by transcript variant 1;
                     CDK2/cyclin A-associated protein p45; p45skp2;
                     F-box/LRR-repeat protein 1; S-phase kinase-associated
                     protein 2, E3 ubiquitin protein ligase"
                     /codon_start=1
                     /product="S-phase kinase-associated protein 2 isoform 1"
                     /protein_id="NP_005974.2"
                     /db_xref="CCDS:CCDS3916.1"
                     /db_xref="GeneID:6502"
                     /db_xref="HGNC:HGNC:10901"
                     /db_xref="MIM:601436"
                     /translation="MHRKHLQEIPDLSSNVATSFTWGWDSSKTSELLSGMGVSALEKE
                     EPDSENIPQELLSNLGHPESPPRKRLKSKGSDKDFVIVRRPKLNRENFPGVSWDSLPD
                     ELLLGIFSCLCLPELLKVSGVCKRWYRLASDESLWQTLDLTGKNLHPDVTGRLLSQGV
                     IAFRCPRSFMDQPLAEHFSPFRVQHMDLSNSVIEVSTLHGILSQCSKLQNLSLEGLRL
                     SDPIVNTLAKNSNLVRLNLSGCSGFSEFALQTLLSSCSRLDELNLSWCFDFTEKHVQV
                     AVAHVSETITQLNLSGYRKNLQKSDLSTLVRRCPNLVHLDLSDSVMLKNDCFQEFFQL
                     NYLQHLSLSRCYDIIPETLLELGEIPTLKTLQVFGIVPDGTLQLLKEALPHLQINCSH
                     FTTIARPTIGNKKNQEIWGIKCRLTLQKPSCL"
     exon            161..432
                     /gene="SKP2"
                     /gene_synonym="FBL1; FBXL1; FLB1; p45"
                     /inference="alignment:Splign:2.1.0"
     exon            433..544
                     /gene="SKP2"
                     /gene_synonym="FBL1; FBXL1; FLB1; p45"
                     /inference="alignment:Splign:2.1.0"
     exon            545..688
                     /gene="SKP2"
                     /gene_synonym="FBL1; FBXL1; FLB1; p45"
                     /inference="alignment:Splign:2.1.0"
     exon            689..823
                     /gene="SKP2"
                     /gene_synonym="FBL1; FBXL1; FLB1; p45"
                     /inference="alignment:Splign:2.1.0"
     exon            824..922
                     /gene="SKP2"
                     /gene_synonym="FBL1; FBXL1; FLB1; p45"
                     /inference="alignment:Splign:2.1.0"
     exon            923..1053
                     /gene="SKP2"
                     /gene_synonym="FBL1; FBXL1; FLB1; p45"
                     /inference="alignment:Splign:2.1.0"
     exon            1054..1105
                     /gene="SKP2"
                     /gene_synonym="FBL1; FBXL1; FLB1; p45"
                     /inference="alignment:Splign:2.1.0"
     exon            1106..1213
                     /gene="SKP2"
                     /gene_synonym="FBL1; FBXL1; FLB1; p45"
                     /inference="alignment:Splign:2.1.0"
     exon            1214..3715
                     /gene="SKP2"
                     /gene_synonym="FBL1; FBXL1; FLB1; p45"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3403..3408
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SKP2"
                     /gene_synonym="FBL1; FBXL1; FLB1; p45"
                     /note="hexamer: AATAAA"
     regulatory      3418..3423
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SKP2"
                     /gene_synonym="FBL1; FBXL1; FLB1; p45"
                     /note="hexamer: TATAAA"
     polyA_site      3425
                     /gene="SKP2"
                     /gene_synonym="FBL1; FBXL1; FLB1; p45"
                     /note="major polyA site"
     polyA_site      3437
                     /gene="SKP2"
                     /gene_synonym="FBL1; FBXL1; FLB1; p45"
     regulatory      3603..3608
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SKP2"
                     /gene_synonym="FBL1; FBXL1; FLB1; p45"
                     /note="hexamer: ATTAAA"
     polyA_site      3636
                     /gene="SKP2"
                     /gene_synonym="FBL1; FBXL1; FLB1; p45"
ORIGIN      
        1 agtggggatg gaacgttgct aggcttagcg ggtctggctg ctgggggccc gagcagcacg
       61 ctcggagccg ccgcgcgcca aagcgggaat ctgggaggcg agcagctctg cagttaatgc
      121 acgtatttta aactcccggg cctgcggacg ctatgcacag gaagcacctc caggagattc
      181 cagacctgag tagcaacgtt gccaccagct tcacgtgggg atgggattcc agcaagactt
      241 ctgaactgct gtcaggcatg ggggtctccg ccctggagaa agaggagccc gacagtgaga
      301 acatccccca ggaactgctc tcaaacctgg gccacccgga gagcccccca cggaaacggc
      361 tgaagagcaa agggagtgac aaagactttg tgattgtccg caggcctaag ctaaatcgag
      421 agaactttcc aggtgtttca tgggactccc ttccggatga gctgctcttg ggaatctttt
      481 cctgtctgtg cctccctgag ctgctaaagg tctctggtgt ttgtaagagg tggtatcgcc
      541 tagcgtctga tgagtctcta tggcagacct tagacctcac aggtaaaaat ctgcacccgg
      601 atgtgactgg tcggttgctg tctcaagggg tgattgcctt ccgctgccca cgatcattta
      661 tggaccaacc attggctgaa catttcagcc cttttcgtgt acagcacatg gacctatcga
      721 actcagttat agaagtgtcc accctccacg gcatactgtc tcagtgttcc aagttgcaga
      781 atctaagcct ggaaggcctg cggctttcgg atcccattgt caatactctc gcaaaaaact
      841 caaatttagt gcgacttaac ctttctgggt gttctggatt ctctgaattt gccctgcaga
      901 ctttgctaag cagctgttcc agactggatg agctgaacct ctcctggtgt tttgatttca
      961 ctgaaaagca tgtacaggtg gctgttgcgc atgtgtcaga gaccatcacc cagctgaatc
     1021 ttagcggcta cagaaagaat ctccagaaat cagatctctc tactttagtt agaagatgcc
     1081 ccaatcttgt ccatctagac ttaagtgata gtgtcatgct aaagaatgac tgctttcagg
     1141 aatttttcca gctcaactac ctccaacacc tatcactcag tcggtgctat gatataatac
     1201 ctgaaacttt acttgaactt ggagaaattc ccacactaaa aacactacaa gtttttggaa
     1261 tcgtgccaga tggtaccctt caactgttaa aggaagccct tcctcatcta cagattaatt
     1321 gctcccattt caccaccatt gccaggccaa ctattggcaa caaaaagaac caggagatat
     1381 ggggcatcaa atgccgactg acactgcaaa agcccagttg tctatgaagt atttattgca
     1441 ggatggtgtc tcttctttag aacagggaaa ataggcagga agcccaattg ctggagtact
     1501 tagctagttt tattcttggt tttccctttg ccttcattct gcaagtatac tagggagcca
     1561 tttgagaggg aaaactatga aatcttgctt tttgaaatga ttctaaaagc ttctatcact
     1621 gctttgctct taagagccaa agttgtaggc cttttgaaat tttaggagag tgagcctata
     1681 atttcaagat accttaaaga gcaaaatttg agccacctct tccaagtgcc cttcttacta
     1741 agtctattca gaatcaagct taaaaattac caccagcaaa caatcttcat agcccatata
     1801 acttttatct atttaatttt atagtattgc tttataagac agcttagaag aacaataagc
     1861 tatttgtatt atgagctgaa caaaaagaga atcataggat agtagcgtct gaggccatct
     1921 tttctaggaa taggaaagag aaaaatgtat ttgaattttg cctttagatt tgaaattagg
     1981 ttaatagaaa taagtaaccc catgtaattc accttaaaac ttaacaaaag accaaacatt
     2041 acaaaaccca gagatataga atcaatatag gatttgaagg cccagcagac agttttctat
     2101 gacaggttaa tctgaagtat cctgtaatgt tcattaagtt actgtgtttc cagaatctaa
     2161 attagatgag aaatataatt gtggttttct aacttgataa tcaaattatg ttaacatggg
     2221 tcctttagct tttaaaatga cttgctttgt tttagaaagg tggtattaat ccactctcta
     2281 ttcttgaaaa tttggatggg agaattctga agttgcctgc tgttttcctt tagcgctgag
     2341 gttcttaagg ttacttttat attactctgg aatcaagtat tttaaattgt attttttttt
     2401 taaatgatct ctcagcaata attgtttgaa actatccata tataaggtta tcagacctac
     2461 agttccctaa gaggaactgc atgttctctt caatcagaaa tatacagtag aagcaggtat
     2521 atcttccatg cagtttcagt agtaagcact acttatacct acataagagt taaaatccag
     2581 atgtgggacc ttttgatacc atcagtgata tatatttttt taaactggta cagagaagtg
     2641 aaaagattaa attctacttc tatttttttt tttttttttt gagacggagt ctcgctctgt
     2701 caccaaggcc ggagtgcagt ggtgcgatct cggctcactg caagctccgc ctcccaggtt
     2761 cacgtcattc tcctgcctca gcctcccgac tagctgggac tacaggcgcc caccaccacg
     2821 cccggctaat ttttttgtat ttttagtaga gacggggttt caccatgtta gccaggatgg
     2881 tctcaatctc ctgacctcat gatccgcccg tcttggcctc ccaaagtgct gggattacag
     2941 gcatgagcaa ctgcgcccag ccaaattcta cttcttaaaa atcacaaaaa ctagtttaaa
     3001 ttgatgactt gttcgtatgt tcaaaatgta acaacaaaaa aagctaacac cagtcattta
     3061 tattaacttt ttttttttaa atcaaaaatt gttaatgtta gaaacatact atgaagtgcc
     3121 tttatctgct tagacctaag gaagatttta aagttgggtt gcacaggaaa tgatgatgct
     3181 tcaatttctt aatagttaaa aagtgctaaa tactacttga aattattgtt tacagattag
     3241 tgacaagagc tggggttagg atccggttgg actctgacat cggatgccct caaacataca
     3301 gaacttccaa actcaagtcc agccataagc tattttgcca acatgtcaga gtaatctgta
     3361 tttttgtatg tgatttctac ttttatagac ttgttttaaa acaataaaac acatttttat
     3421 aaaaatgagt gcttaaacta agttgtattc cttttttctt tctctttttt taagtgctgt
     3481 ggttaaaatt tgaaagcatt tagtgtggta tgccatttgg cttagatacc tctaaataag
     3541 actcttcaaa acagagccct gagatggtcc tttttgaccc atctacttca tatgcttgtc
     3601 acattaaaaa aaaaagttta taatgccttt ataaaagggg ctaatacagt cttgttatct
     3661 ttttttttaa attatacttt aagttctagg gtacatgtgc acaacgtgca ggttt
//
