LOCUS       NM_025234               1202 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens SKI8 subunit of superkiller complex (SKIC8),
            transcript variant 2, mRNA.
ACCESSION   NM_025234
VERSION     NM_025234.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Kogel,A., Keidel,A., Bonneau,F., Schafer,I.B. and Conti,E.
  TITLE     The human SKI complex regulates channeling of ribosome-bound RNA to
            the exosome via an intrinsic gatekeeping mechanism
  JOURNAL   Mol Cell 82 (4), 756-769 (2022)
   PUBMED   35120588
REFERENCE   2  (bases 1 to 1202)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 1202)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   4  (bases 1 to 1202)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 1202)
  AUTHORS   Zinoviev,A., Ayupov,R.K., Abaeva,I.S., Hellen,C.U.T. and
            Pestova,T.V.
  TITLE     Extraction of mRNA from Stalled Ribosomes by the Ski Complex
  JOURNAL   Mol Cell 77 (6), 1340-1349 (2020)
   PUBMED   32006463
REFERENCE   6  (bases 1 to 1202)
  AUTHORS   Muntean,A.G., Tan,J., Sitwala,K., Huang,Y., Bronstein,J.,
            Connelly,J.A., Basrur,V., Elenitoba-Johnson,K.S. and Hess,J.L.
  TITLE     The PAF complex synergizes with MLL fusion proteins at HOX loci to
            promote leukemogenesis
  JOURNAL   Cancer Cell 17 (6), 609-621 (2010)
   PUBMED   20541477
REFERENCE   7  (bases 1 to 1202)
  AUTHORS   Kim,J., Guermah,M. and Roeder,R.G.
  TITLE     The human PAF1 complex acts in chromatin transcription elongation
            both independently and cooperatively with SII/TFIIS
  JOURNAL   Cell 140 (4), 491-503 (2010)
   PUBMED   20178742
REFERENCE   8  (bases 1 to 1202)
  AUTHORS   Tegha-Dunghu,J., Neumann,B., Reber,S., Krause,R., Erfle,H.,
            Walter,T., Held,M., Rogers,P., Hupfeld,K., Ruppert,T., Ellenberg,J.
            and Gruss,O.J.
  TITLE     EML3 is a nuclear microtubule-binding protein required for the
            correct alignment of chromosomes in metaphase
  JOURNAL   J Cell Sci 121 (Pt 10), 1718-1726 (2008)
   PUBMED   18445686
REFERENCE   9  (bases 1 to 1202)
  AUTHORS   Zhu,B., Mandal,S.S., Pham,A.D., Zheng,Y., Erdjument-Bromage,H.,
            Batra,S.K., Tempst,P. and Reinberg,D.
  TITLE     The human PAF complex coordinates transcription with events
            downstream of RNA synthesis
  JOURNAL   Genes Dev 19 (14), 1668-1673 (2005)
   PUBMED   16024656
REFERENCE   10 (bases 1 to 1202)
  AUTHORS   Barrios-Rodiles,M., Brown,K.R., Ozdamar,B., Bose,R., Liu,Z.,
            Donovan,R.S., Shinjo,F., Liu,Y., Dembowy,J., Taylor,I.W., Luga,V.,
            Przulj,N., Robinson,M., Suzuki,H., Hayashizaki,Y., Jurisica,I. and
            Wrana,J.L.
  TITLE     High-throughput mapping of a dynamic signaling network in mammalian
            cells
  JOURNAL   Science 307 (5715), 1621-1625 (2005)
   PUBMED   15761153
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BI916225.1 and AK024754.1.
            
            On Nov 24, 2018 this sequence version replaced NM_025234.2.
            
            Summary: WDR61 is a subunit of the human PAF and SKI complexes,
            which function in transcriptional regulation and are involved in
            events downstream of RNA synthesis, such as RNA surveillance (Zhu
            et al., 2005 [PubMed 16024656]).[supplied by OMIM, Mar 2008].
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1 and 2 both encode the same isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.418005.1,
                                           SRR3476690.953537.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000267973.7/ ENSP00000267973.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-165               BI916225.1         118-282
            166-1202            AK024754.1         155-1191
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q25.1"
     gene            1..1202
                     /gene="SKIC8"
                     /gene_synonym="REC14; SKI8; WDR61"
                     /note="SKI8 subunit of superkiller complex"
                     /db_xref="GeneID:80349"
                     /db_xref="HGNC:HGNC:30300"
                     /db_xref="MIM:609540"
     exon            1..48
                     /gene="SKIC8"
                     /gene_synonym="REC14; SKI8; WDR61"
                     /inference="alignment:Splign:2.1.0"
     exon            49..99
                     /gene="SKIC8"
                     /gene_synonym="REC14; SKI8; WDR61"
                     /inference="alignment:Splign:2.1.0"
     CDS             88..1005
                     /gene="SKIC8"
                     /gene_synonym="REC14; SKI8; WDR61"
                     /note="isoform a is encoded by transcript variant 2;
                     recombination protein REC14; WD repeat-containing protein
                     61; meiotic recombination REC14 protein homolog; SKI8
                     homolog; SKI complex component SKI8; Ski complex subunit
                     Rec14; WD repeat domain 61; SKI8 subunit of superkiller
                     complex protein; superkiller complex protein 8"
                     /codon_start=1
                     /product="superkiller complex protein 8 isoform a"
                     /protein_id="NP_079510.1"
                     /db_xref="CCDS:CCDS10300.1"
                     /db_xref="GeneID:80349"
                     /db_xref="HGNC:HGNC:30300"
                     /db_xref="MIM:609540"
                     /translation="MTNQYGILFKQEQAHDDAIWSVAWGTNKKENSETVVTGSLDDLV
                     KVWKWRDERLDLQWSLEGHQLGVVSVDISHTLPIAASSSLDAHIRLWDLENGKQIKSI
                     DAGPVDAWTLAFSPDSQYLATGTHVGKVNIFGVESGKKEYSLDTRGKFILSIAYSPDG
                     KYLASGAIDGIINIFDIATGKLLHTLEGHAMPIRSLTFSPDSQLLVTASDDGYIKIYD
                     VQHANLAGTLSGHASWVLNVAFCPDDTHFVSSSSDKSVKVWDVGTRTCVHTFFDHQDQ
                     VWGVKYNGNGSKIVSVGDDQEIHIYDCPI"
     exon            100..127
                     /gene="SKIC8"
                     /gene_synonym="REC14; SKI8; WDR61"
                     /inference="alignment:Splign:2.1.0"
     exon            128..233
                     /gene="SKIC8"
                     /gene_synonym="REC14; SKI8; WDR61"
                     /inference="alignment:Splign:2.1.0"
     exon            234..406
                     /gene="SKIC8"
                     /gene_synonym="REC14; SKI8; WDR61"
                     /inference="alignment:Splign:2.1.0"
     exon            407..555
                     /gene="SKIC8"
                     /gene_synonym="REC14; SKI8; WDR61"
                     /inference="alignment:Splign:2.1.0"
     exon            556..649
                     /gene="SKIC8"
                     /gene_synonym="REC14; SKI8; WDR61"
                     /inference="alignment:Splign:2.1.0"
     exon            650..743
                     /gene="SKIC8"
                     /gene_synonym="REC14; SKI8; WDR61"
                     /inference="alignment:Splign:2.1.0"
     exon            744..836
                     /gene="SKIC8"
                     /gene_synonym="REC14; SKI8; WDR61"
                     /inference="alignment:Splign:2.1.0"
     exon            837..915
                     /gene="SKIC8"
                     /gene_synonym="REC14; SKI8; WDR61"
                     /inference="alignment:Splign:2.1.0"
     exon            916..1202
                     /gene="SKIC8"
                     /gene_synonym="REC14; SKI8; WDR61"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1181..1186
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SKIC8"
                     /gene_synonym="REC14; SKI8; WDR61"
                     /note="hexamer: CATAAA"
     polyA_site      1202
                     /gene="SKIC8"
                     /gene_synonym="REC14; SKI8; WDR61"
                     /note="major polyA site"
ORIGIN      
        1 gcagtcgcgc ggagcccggc ttccgacgtg cagcctggca gtgcagtgag ctgtctggcc
       61 ttttgtcctt gatccttggt taaggaaatg accaaccagt acggtattct cttcaaacaa
      121 gagcaagccc atgatgatgc catttggtca gttgcttggg ggacaaacaa gaaggaaaac
      181 tctgagacag tggtcacagg ctccctagat gacctggtga aggtctggaa atggcgtgat
      241 gagaggctgg acctacagtg gagtctggag ggacatcagc tgggagtggt gtctgtggac
      301 atcagccaca ccctgcccat tgctgcatcc agctctcttg atgctcatat tcgtctttgg
      361 gacttggaaa atggcaaaca gataaagtcc atagatgcag gacctgtgga tgcctggact
      421 ttggcctttt ctcctgattc ccagtatctg gccacaggaa ctcatgtcgg gaaagtgaac
      481 atttttggtg tggaaagtgg gaaaaaggaa tattctttgg acacgagagg aaaattcatt
      541 cttagtattg catatagtcc tgatgggaaa tacctagcca gtggagccat agatggaatc
      601 atcaatattt ttgatattgc aactggaaaa cttctgcata ccctggaagg ccatgccatg
      661 cccattcgct ccttgacctt ttccccggac tcccagctcc ttgtcactgc ttcagatgat
      721 ggctacatca agatctatga tgtacaacat gccaatttgg ctggcacgct gagcggccat
      781 gcctcctggg tgctgaacgt tgcattctgt cctgatgaca ctcactttgt ttccagttcg
      841 tctgacaaaa gtgtaaaagt ttgggatgtt ggaacgagga cttgtgttca caccttcttt
      901 gatcaccagg atcaggtctg gggagtaaaa tacaatggaa atggttcaaa aattgtgtct
      961 gttggagatg accaggaaat tcacatctat gattgtccaa tttaaacatc aaagtctcca
     1021 ggcttatgct gcaaagagaa tgtacggatt gatcatgaca ttccttacct tcttaggctt
     1081 gtttaaaaga aatatagcat ttattgtagc aaagacttaa attttgtaga tacaatatga
     1141 atcttttcat gttttattgg aaatgctgtt catactttaa cataaagctt tcttaatgca
     1201 aa
//
