LOCUS       NM_006929               3795 bp    mRNA    linear   PRI 07-FEB-2025
DEFINITION  Homo sapiens SKI2 subunit of superkiller complex (SKIC2), mRNA.
ACCESSION   NM_006929
VERSION     NM_006929.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Kogel,A., Keidel,A., Bonneau,F., Schafer,I.B. and Conti,E.
  TITLE     The human SKI complex regulates channeling of ribosome-bound RNA to
            the exosome via an intrinsic gatekeeping mechanism
  JOURNAL   Mol Cell 82 (4), 756-769 (2022)
   PUBMED   35120588
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 3795)
  AUTHORS   Zinoviev,A., Ayupov,R.K., Abaeva,I.S., Hellen,C.U.T. and
            Pestova,T.V.
  TITLE     Extraction of mRNA from Stalled Ribosomes by the Ski Complex
  JOURNAL   Mol Cell 77 (6), 1340-1349 (2020)
   PUBMED   32006463
REFERENCE   4  (bases 1 to 3795)
  AUTHORS   Bourgeois,P., Esteve,C., Chaix,C., Beroud,C., Levy,N., Fabre,A. and
            Badens,C.
  CONSRTM   THES clinical consortium
  TITLE     Tricho-Hepato-Enteric Syndrome mutation update: Mutations spectrum
            of TTC37 and SKIV2L, clinical analysis and future prospects
  JOURNAL   Hum Mutat 39 (6), 774-789 (2018)
   PUBMED   29527791
  REMARK    GeneRIF: We then examined the lab diagnostic cohort in detail for
            clinical manifestations. For the first time, we are able to suggest
            that patients lacking SKIV2L seem more severely affected than those
            lacking TTC37, in terms of liver damage and prenatal growth
            impairment.
            Review article
REFERENCE   5  (bases 1 to 3795)
  AUTHORS   Shuai,P., Ye,Z., Liu,Y., Qu,C., Liu,X., Luo,H., Feng,X., Li,X.,
            Shi,Y. and Gong,B.
  TITLE     Association between SKIV2L polymorphism rs429608 and age-related
            macular degeneration: A meta-analysis
  JOURNAL   Ophthalmic Genet 38 (3), 245-251 (2017)
   PUBMED   27484132
  REMARK    GeneRIF: This meta-analysis showed that SKIV2L rs429608 was
            statistically associated with age-related macular degeneration(AMD)
            and it might exert a protective effect on AMD. Further
            investigations are needed to validate the association and confirm
            the role of SKIV2L in AMD.
            Erratum:[Ophthalmic Genet. 2017 May-Jun;38(3):300. doi:
            10.1080/13816810.2016.1240548.. PMID: 28095096]
REFERENCE   6  (bases 1 to 3795)
  AUTHORS   Albertella,M.R., Jones,H., Thomson,W., Olavesen,M.G. and
            Campbell,R.D.
  TITLE     Localization of eight additional genes in the human major
            histocompatibility complex, including the gene encoding the casein
            kinase II beta subunit (CSNK2B)
  JOURNAL   Genomics 36 (2), 240-251 (1996)
   PUBMED   8812450
REFERENCE   7  (bases 1 to 3795)
  AUTHORS   Dangel,A.W., Shen,L., Mendoza,A.R., Wu,L.C. and Yu,C.Y.
  TITLE     Human helicase gene SKI2W in the HLA class III region exhibits
            striking structural similarities to the yeast antiviral gene SKI2
            and to the human gene KIAA0052: emergence of a new gene family
  JOURNAL   Nucleic Acids Res 23 (12), 2120-2126 (1995)
   PUBMED   7610041
REFERENCE   8  (bases 1 to 3795)
  AUTHORS   Lee,S.G., Lee,I., Park,S.H., Kang,C. and Song,K.
  TITLE     Identification and characterization of a human cDNA homologous to
            yeast SKI2
  JOURNAL   Genomics 25 (3), 660-666 (1995)
   PUBMED   7759100
REFERENCE   9  (bases 1 to 3795)
  AUTHORS   Shen,L., Wu,L.C., Sanlioglu,S., Chen,R., Mendoza,A.R., Dangel,A.W.,
            Carroll,M.C., Zipf,W.B. and Yu,C.Y.
  TITLE     Structure and genetics of the partially duplicated gene RP located
            immediately upstream of the complement C4A and the C4B genes in the
            HLA class III region. Molecular cloning, exon-intron structure,
            composite retroposon, and breakpoint of gene duplication
  JOURNAL   J Biol Chem 269 (11), 8466-8476 (1994)
   PUBMED   8132574
REFERENCE   10 (bases 1 to 3795)
  AUTHORS   Fabre,A., Bourgeois,P., Chaix,C., Bertaux,K., Goulet,O. and
            Badens,C.
  TITLE     Trichohepatoenteric Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   29334452
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from U09877.1, AL529319.3,
            BG396326.1, BE395851.1 and Z48796.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_006929.4.
            
            Summary: DEAD box proteins, characterized by the conserved motif
            Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are
            implicated in a number of cellular processes involving alteration
            of RNA secondary structure such as translation initiation, nuclear
            and mitochondrial splicing, and ribosome and spliceosome assembly.
            Based on their distribution patterns, some members of this family
            are believed to be involved in embryogenesis, spermatogenesis, and
            cellular growth and division. This gene encodes a DEAD box protein,
            which is a human homologue of yeast SKI2 and may be involved in
            antiviral activity by blocking translation of poly(A) deficient
            mRNAs. This gene is located in the class III region of the major
            histocompatibility complex. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: Z48796.1, U09877.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000375394.7/ ENSP00000364543.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-654               U09877.1           94-747
            655-662             AL529319.3         646-653
            663-1111            U09877.1           756-1204
            1112-1120           BG396326.1         396-404
            1121-2910           U09877.1           1214-3003
            2911-2917           BE395851.1         513-519
            2918-3741           U09877.1           3011-3834
            3742-3748           Z48796.1           3776-3782
            3749-3795           U09877.1           3842-3888
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6p21.33"
     gene            1..3795
                     /gene="SKIC2"
                     /gene_synonym="170A; DDX13; HLP; SKI2; SKI2W; SKIV2;
                     SKIV2L; SKIV2L1; THES2"
                     /note="SKI2 subunit of superkiller complex"
                     /db_xref="GeneID:6499"
                     /db_xref="HGNC:HGNC:10898"
                     /db_xref="MIM:600478"
     exon            1..40
                     /gene="SKIC2"
                     /gene_synonym="170A; DDX13; HLP; SKI2; SKI2W; SKIV2;
                     SKIV2L; SKIV2L1; THES2"
                     /inference="alignment:Splign:2.1.0"
     CDS             19..3759
                     /gene="SKIC2"
                     /gene_synonym="170A; DDX13; HLP; SKI2; SKI2W; SKIV2;
                     SKIV2L; SKIV2L1; THES2"
                     /EC_number="3.6.4.13"
                     /note="helicase SKI2W; helicase-like protein; superkiller
                     viralicidic activity 2-like; SKI2 homolog, superkiller
                     viralicidic activity 2-like; Ski2 like RNA helicase; SKI2
                     subunit of superkiller complex protein"
                     /codon_start=1
                     /product="superkiller complex protein 2"
                     /protein_id="NP_008860.4"
                     /db_xref="CCDS:CCDS4731.1"
                     /db_xref="GeneID:6499"
                     /db_xref="HGNC:HGNC:10898"
                     /db_xref="MIM:600478"
                     /translation="MMETERLVLPPPDPLDLPLRAVELGCTGHWELLNLPGAPESSLP
                     HGLPPCAPDLQQEAEQLFLSSPAWLPLHGVEHSARKWQRKTDPWSLLAVLGAPVPSDL
                     QAQRHPTTGQILGYKEVLLENTNLSATTSLSLRRPPGPASQSLWGNPTQYPFWPGGMD
                     EPTITDLNTREEAEEEIDFEKDLLTIPPGFKKGMDFAPKDCPTPAPGLLSLSCMLEPL
                     DLGGGDEDENEAVGQPGGPRGDTVSASPCSAPLARASSLEDLVLKEASTAVSTPEAPE
                     PPSQEQWAIPVDATSPVGDFYRLIPQPAFQWAFEPDVFQKQAILHLERHDSVFVAAHT
                     SAGKTVVAEYAIALAQKHMTRTIYTSPIKALSNQKFRDFRNTFGDVGLLTGDVQLHPE
                     ASCLIMTTEILRSMLYSGSDVIRDLEWVIFDEVHYINDVERGVVWEEVLIMLPDHVSI
                     ILLSATVPNALEFADWIGRLKRRQIYVISTVTRPVPLEHYLFTGNSSKTQGELFLLLD
                     SRGAFHTKGYYAAVEAKKERMSKHAQTFGAKQPTHQGGPAQDRGVYLSLLASLRTRAQ
                     LPVVVFTFSRGRCDEQASGLTSLDLTTSSEKSEIHLFLQRCLARLRGSDRQLPQVLHM
                     SELLNRGLGVHHSGILPILKEIVEMLFSRGLVKVLFATETFAMGVNMPARTVVFDSMR
                     KHDGSTFRDLLPGEYVQMAGRAGRRGLDPTGTVILLCKGRVPEMADLHRMMMGKPSQL
                     QSQFRLTYTMILNLLRVDALRVEDMMKRSFSEFPSRKDSKAHEQALAELTKRLGALEE
                     PDMTGQLVDLPEYYSWGEELTETQHMIQRRIMESVNGLKSLSAGRVVVVKNQEHHNAL
                     GVILQVSSNSTSRVFTTLVLCDKPLSQDPQDRGPATAEVPYPDDLVGFKLFLPEGPCD
                     HTVVKLQPGDMAAITTKVLRVNGEKILEDFSKRQQPKFKKDPPLAAVTTAVQELLRLA
                     QAHPAGPPTLDPVNDLQLKDMSVVEGGLRARKLEELIQGAQCVHSPRFPAQYLKLRER
                     MQIQKEMERLRFLLSDQSLLLLPEYHQRVEVLRTLGYVDEAGTVKLAGRVACAMSSHE
                     LLLTELMFDNALSTLRPEEIAALLSGLVCQSPGDAGDQLPNTLKQGIERVRAVAKRIG
                     EVQVACGLNQTVEEFVGELNFGLVEVVYEWARGMPFSELAGLSGTPEGLVVRCIQRLA
                     EMCRSLRGAARLVGEPVLGAKMETAATLLRRDIVFAASLYTQ"
     exon            41..144
                     /gene="SKIC2"
                     /gene_synonym="170A; DDX13; HLP; SKI2; SKI2W; SKIV2;
                     SKIV2L; SKIV2L1; THES2"
                     /inference="alignment:Splign:2.1.0"
     exon            145..254
                     /gene="SKIC2"
                     /gene_synonym="170A; DDX13; HLP; SKI2; SKI2W; SKIV2;
                     SKIV2L; SKIV2L1; THES2"
                     /inference="alignment:Splign:2.1.0"
     exon            255..372
                     /gene="SKIC2"
                     /gene_synonym="170A; DDX13; HLP; SKI2; SKI2W; SKIV2;
                     SKIV2L; SKIV2L1; THES2"
                     /inference="alignment:Splign:2.1.0"
     exon            373..487
                     /gene="SKIC2"
                     /gene_synonym="170A; DDX13; HLP; SKI2; SKI2W; SKIV2;
                     SKIV2L; SKIV2L1; THES2"
                     /inference="alignment:Splign:2.1.0"
     exon            488..562
                     /gene="SKIC2"
                     /gene_synonym="170A; DDX13; HLP; SKI2; SKI2W; SKIV2;
                     SKIV2L; SKIV2L1; THES2"
                     /inference="alignment:Splign:2.1.0"
     exon            563..616
                     /gene="SKIC2"
                     /gene_synonym="170A; DDX13; HLP; SKI2; SKI2W; SKIV2;
                     SKIV2L; SKIV2L1; THES2"
                     /inference="alignment:Splign:2.1.0"
     exon            617..807
                     /gene="SKIC2"
                     /gene_synonym="170A; DDX13; HLP; SKI2; SKI2W; SKIV2;
                     SKIV2L; SKIV2L1; THES2"
                     /inference="alignment:Splign:2.1.0"
     exon            808..936
                     /gene="SKIC2"
                     /gene_synonym="170A; DDX13; HLP; SKI2; SKI2W; SKIV2;
                     SKIV2L; SKIV2L1; THES2"
                     /inference="alignment:Splign:2.1.0"
     exon            937..1082
                     /gene="SKIC2"
                     /gene_synonym="170A; DDX13; HLP; SKI2; SKI2W; SKIV2;
                     SKIV2L; SKIV2L1; THES2"
                     /inference="alignment:Splign:2.1.0"
     exon            1083..1229
                     /gene="SKIC2"
                     /gene_synonym="170A; DDX13; HLP; SKI2; SKI2W; SKIV2;
                     SKIV2L; SKIV2L1; THES2"
                     /inference="alignment:Splign:2.1.0"
     exon            1230..1314
                     /gene="SKIC2"
                     /gene_synonym="170A; DDX13; HLP; SKI2; SKI2W; SKIV2;
                     SKIV2L; SKIV2L1; THES2"
                     /inference="alignment:Splign:2.1.0"
     exon            1315..1421
                     /gene="SKIC2"
                     /gene_synonym="170A; DDX13; HLP; SKI2; SKI2W; SKIV2;
                     SKIV2L; SKIV2L1; THES2"
                     /inference="alignment:Splign:2.1.0"
     exon            1422..1568
                     /gene="SKIC2"
                     /gene_synonym="170A; DDX13; HLP; SKI2; SKI2W; SKIV2;
                     SKIV2L; SKIV2L1; THES2"
                     /inference="alignment:Splign:2.1.0"
     exon            1569..1665
                     /gene="SKIC2"
                     /gene_synonym="170A; DDX13; HLP; SKI2; SKI2W; SKIV2;
                     SKIV2L; SKIV2L1; THES2"
                     /inference="alignment:Splign:2.1.0"
     exon            1666..1878
                     /gene="SKIC2"
                     /gene_synonym="170A; DDX13; HLP; SKI2; SKI2W; SKIV2;
                     SKIV2L; SKIV2L1; THES2"
                     /inference="alignment:Splign:2.1.0"
     exon            1879..1989
                     /gene="SKIC2"
                     /gene_synonym="170A; DDX13; HLP; SKI2; SKI2W; SKIV2;
                     SKIV2L; SKIV2L1; THES2"
                     /inference="alignment:Splign:2.1.0"
     exon            1990..2220
                     /gene="SKIC2"
                     /gene_synonym="170A; DDX13; HLP; SKI2; SKI2W; SKIV2;
                     SKIV2L; SKIV2L1; THES2"
                     /inference="alignment:Splign:2.1.0"
     exon            2221..2358
                     /gene="SKIC2"
                     /gene_synonym="170A; DDX13; HLP; SKI2; SKI2W; SKIV2;
                     SKIV2L; SKIV2L1; THES2"
                     /inference="alignment:Splign:2.1.0"
     exon            2359..2496
                     /gene="SKIC2"
                     /gene_synonym="170A; DDX13; HLP; SKI2; SKI2W; SKIV2;
                     SKIV2L; SKIV2L1; THES2"
                     /inference="alignment:Splign:2.1.0"
     exon            2497..2601
                     /gene="SKIC2"
                     /gene_synonym="170A; DDX13; HLP; SKI2; SKI2W; SKIV2;
                     SKIV2L; SKIV2L1; THES2"
                     /inference="alignment:Splign:2.1.0"
     exon            2602..2749
                     /gene="SKIC2"
                     /gene_synonym="170A; DDX13; HLP; SKI2; SKI2W; SKIV2;
                     SKIV2L; SKIV2L1; THES2"
                     /inference="alignment:Splign:2.1.0"
     exon            2750..2876
                     /gene="SKIC2"
                     /gene_synonym="170A; DDX13; HLP; SKI2; SKI2W; SKIV2;
                     SKIV2L; SKIV2L1; THES2"
                     /inference="alignment:Splign:2.1.0"
     exon            2877..3087
                     /gene="SKIC2"
                     /gene_synonym="170A; DDX13; HLP; SKI2; SKI2W; SKIV2;
                     SKIV2L; SKIV2L1; THES2"
                     /inference="alignment:Splign:2.1.0"
     exon            3088..3198
                     /gene="SKIC2"
                     /gene_synonym="170A; DDX13; HLP; SKI2; SKI2W; SKIV2;
                     SKIV2L; SKIV2L1; THES2"
                     /inference="alignment:Splign:2.1.0"
     exon            3199..3417
                     /gene="SKIC2"
                     /gene_synonym="170A; DDX13; HLP; SKI2; SKI2W; SKIV2;
                     SKIV2L; SKIV2L1; THES2"
                     /inference="alignment:Splign:2.1.0"
     exon            3418..3558
                     /gene="SKIC2"
                     /gene_synonym="170A; DDX13; HLP; SKI2; SKI2W; SKIV2;
                     SKIV2L; SKIV2L1; THES2"
                     /inference="alignment:Splign:2.1.0"
     exon            3559..3795
                     /gene="SKIC2"
                     /gene_synonym="170A; DDX13; HLP; SKI2; SKI2W; SKIV2;
                     SKIV2L; SKIV2L1; THES2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3782..3787
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SKIC2"
                     /gene_synonym="170A; DDX13; HLP; SKI2; SKI2W; SKIV2;
                     SKIV2L; SKIV2L1; THES2"
                     /note="hexamer: GATAAA"
     polyA_site      3795
                     /gene="SKIC2"
                     /gene_synonym="170A; DDX13; HLP; SKI2; SKI2W; SKIV2;
                     SKIV2L; SKIV2L1; THES2"
                     /note="major polyA site"
ORIGIN      
        1 agctgctgtg gctccaggat gatggagaca gagcgacttg tgctaccccc tccagatccc
       61 ctggacctac cccttcgggc cgtggagctc ggatgcacgg ggcactggga gctgctgaac
      121 ttgcctggag ctccagagag tagccttccc catggcctcc ctccttgtgc cccagatctg
      181 cagcaagaag cagaacagtt gtttctgtca tccccagcct ggctgcctct gcatggtgtg
      241 gagcactcag cccgaaaatg gcagaggaag acggatccct ggtctctttt ggctgtcctg
      301 ggagccccag tcccatccga cctacaggcc caaagacacc caaccacagg ccagatactg
      361 ggttacaaag aggtcttgct ggagaacaca aatctctcgg ctacaacctc cttgtctctt
      421 cgccggcctc cagggccagc ctcccagtcc ttatggggaa atccaactca gtatcccttc
      481 tggccagggg ggatggatga acccaccata acagatctga acacacggga ggaggctgag
      541 gaggagatag actttgagaa agatcttctt actattccac ctggtttcaa gaaaggcatg
      601 gactttgcac caaaagattg tccaactcca gctcctggac tactaagcct tagctgtatg
      661 ttggagcctc tggatttggg tgggggtgac gaggatgaga atgaggcagt gggacagcca
      721 ggaggtccca gaggggacac tgtttcagcc tctccctgca gtgctcccct ggcccgagca
      781 agcagcttgg aagacctagt gttgaaggaa gcgtccacag ctgtatccac cccagaggcc
      841 ccagagcctc catctcagga gcagtgggcc atccctgtgg acgccacctc ccctgttggt
      901 gatttctatc gcctcattcc ccagccagcc ttccagtggg catttgagcc agatgtgttt
      961 cagaaacagg ccatcctgca cttggaacgg catgactctg tctttgtcgc agctcacaca
     1021 tctgcaggaa aaacagttgt ggctgaatat gccattgccc tggcccagaa acacatgaca
     1081 cgcaccatct acacttcgcc catcaaggcc ctgagcaacc agaagttccg ggacttccga
     1141 aacacattcg gggatgtggg gctgctcacc ggggatgtac agctgcatcc ggaggcctcc
     1201 tgcctcatca tgaccacaga gatccttcgc tccatgctgt acagtggctc agatgttatt
     1261 cgggacctgg agtgggtcat ctttgatgag gttcactata tcaacgatgt cgagcgtggg
     1321 gtcgtgtggg aggaggtgct tatcatgcta cctgaccacg tttctatcat ccttctgagt
     1381 gccaccgtcc ccaacgccct tgagtttgct gactggattg ggcggctgaa gcgtcgtcag
     1441 atctatgtga ttagcactgt aacccgcccc gtgcccctgg agcactatct tttcacaggg
     1501 aacagctcca agacccaggg ggagctcttt ttgttgctgg actcccgagg agccttccat
     1561 acaaaagggt actatgcagc tgtggaggcc aagaaggaga gaatgagcaa acacgcccag
     1621 acctttgggg ccaagcagcc cacacatcag gggggccctg cacaggaccg cggagtgtac
     1681 ctgtccctcc tggcctccct ccgcacacgt gcccagttgc ccgtggtggt gttcaccttc
     1741 tcccggggcc gctgtgatga gcaggcctca ggcctcacct cccttgacct caccaccagt
     1801 tcggagaaga gcgagatcca cctcttcctg cagcgctgcc ttgctcgcct ccgtggctct
     1861 gaccgccagc tgccccaggt cctgcacatg tcagagctcc tgaatcgcgg cctgggtgtg
     1921 caccatagcg gcatcctgcc catcctcaag gagatcgtgg agatgctctt cagccgtggc
     1981 ctggtcaagg tcttgtttgc cacagagacc tttgccatgg gagtaaacat gcctgctcgt
     2041 acagtagtgt ttgactccat gcgcaaacac gatggctcca ccttccggga cctgctccct
     2101 ggggagtatg tgcagatggc aggccgggca gggcggaggg gcctggaccc cacaggcacc
     2161 gttatcctgc tctgcaaggg ccgagtgccc gagatggcag acctgcaccg catgatgatg
     2221 gggaagccgt cccagctgca gtcccagttc cgcctcacgt acactatgat cctcaacttg
     2281 ctgcgagtgg atgccctcag ggtggaggac atgatgaaga ggagcttctc tgagtttccc
     2341 tcccgcaaag acagcaaggc ccatgaacag gccctggctg aactgaccaa gaggctggga
     2401 gctttggagg agcctgacat gactggccaa ctggtcgacc tgcctgaata ttacagctgg
     2461 ggggaggaac tgacagagac ccagcacatg atccagcgac gcatcatgga gtctgtgaac
     2521 gggctgaagt ctctctcagc aggaagggtg gtggttgtga agaatcagga gcatcacaac
     2581 gcattgggag tgatcctaca ggtctcctcg aactccacca gcagagtatt cacaaccctg
     2641 gtcttgtgtg ataagccctt gtcccaggac ccacaggaca gggggccagc cactgcagag
     2701 gtgccctatc cagatgacct cgtgggattc aagctgttcc tgcctgaagg gccttgtgac
     2761 cacaccgtgg tcaagctcca gccaggagat atggctgcca tcaccaccaa ggtgctccgg
     2821 gtgaatgggg agaagatctt ggaggacttc agcaagaggc agcagccaaa attcaagaag
     2881 gatcctcccc ttgcagccgt gaccactgct gtccaggaac tgctgcgtct ggctcaggcc
     2941 cacccagccg gacctcccac cctcgaccct gtcaatgacc tgcagctcaa agatatgtca
     3001 gttgtagagg gtgggctccg ggcccggaag ctggaggagc tgatccaggg ggctcagtgt
     3061 gtacacagcc cccgttttcc tgcccagtac ctgaagctgc gggagcgaat gcagatacag
     3121 aaggagatgg agcggctgcg cttcctactg tcggatcagt cattgctgct gcttcctgag
     3181 taccatcagc gagtagaggt gctccgaacc ctgggttatg tggacgaggc gggcactgtg
     3241 aagctggcag ggcgggtggc ttgtgccatg agcagccatg agttgctcct cactgagctc
     3301 atgtttgaca atgcactgag caccctgcgg cctgaggaga ttgctgcctt gctctctggc
     3361 ctggtctgcc agagccctgg ggacgctggg gatcagctcc caaacaccct caagcaggga
     3421 atagaacgtg tccgggctgt ggccaagcgg attggtgagg tccaggtggc ttgtggcctg
     3481 aaccagacgg tggaggaatt tgtgggggag ctgaattttg ggctggttga ggttgtatat
     3541 gagtgggccc ggggcatgcc cttctccgag ttggcagggc tctcagggac ccctgagggc
     3601 ctggtggtcc gctgcattca gcgcctggct gagatgtgtc gctcactgcg gggggcagcc
     3661 cgcctggtag gagagcctgt gctgggtgcc aagatggaga cagcggctac cttgctacgg
     3721 cgggacatcg tatttgcggc cagcctctac acccagtgaa tgccccatgt aaaaacatga
     3781 tgataaaaca gcaaa
//
