LOCUS       NM_003930               3839 bp    mRNA    linear   PRI 23-MAR-2024
DEFINITION  Homo sapiens src kinase associated phosphoprotein 2 (SKAP2),
            transcript variant 1, mRNA.
ACCESSION   NM_003930
VERSION     NM_003930.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Bouti,P., Klein,B.J.A.M., Verkuijlen,P.J.H., Schornagel,K., van
            Alphen,F.P.J., Taris,K.H., van den Biggelaar,M., Hoogendijk,A.J.,
            van Bruggen,R., Kuijpers,T.W. and Matlung,H.L.
  TITLE     SKAP2 acts downstream of CD11b/CD18 and regulates neutrophil
            effector function
  JOURNAL   Front Immunol 15, 1344761 (2024)
   PUBMED   38487529
  REMARK    GeneRIF: SKAP2 acts downstream of CD11b/CD18 and regulates
            neutrophil effector function.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   Jin,X., Chen,Y., Sheng,Z., Wang,X., Zhang,Z., Huang,J., Zhou,J. and
            Lin,F.
  TITLE     SKAP2 is downregulated in the villous tissues of patients with
            missed abortion and regulates growth and migration in trophoblasts
            through the WAVE2-ARP2/3 signaling pathway
  JOURNAL   Placenta 128, 100-111 (2022)
   PUBMED   36126383
  REMARK    GeneRIF: SKAP2 is downregulated in the villous tissues of patients
            with missed abortion and regulates growth and migration in
            trophoblasts through the WAVE2-ARP2/3 signaling pathway.
REFERENCE   3  (bases 1 to 3839)
  AUTHORS   Rutsch,N., Chamberlain,C.E., Dixon,W., Spector,L.,
            Letourneau-Freiberg,L.R., Lwin,W.W., Philipson,L.H., Zarbock,A.,
            Saintus,K., Wang,J., German,M.S., Anderson,M.S. and Lowell,C.A.
  TITLE     Diabetes With Multiple Autoimmune and Inflammatory Conditions
            Linked to an Activating SKAP2 Mutation
  JOURNAL   Diabetes Care 44 (8), 1816-1825 (2021)
   PUBMED   34172489
  REMARK    GeneRIF: Diabetes With Multiple Autoimmune and Inflammatory
            Conditions Linked to an Activating SKAP2 Mutation.
REFERENCE   4  (bases 1 to 3839)
  AUTHORS   Dadwal,N., Mix,C., Reinhold,A., Witte,A., Freund,C., Schraven,B.
            and Kliche,S.
  TITLE     The Multiple Roles of the Cytosolic Adapter Proteins ADAP, SKAP1
            and SKAP2 for TCR/CD3 -Mediated Signaling Events
  JOURNAL   Front Immunol 12, 703534 (2021)
   PUBMED   34295339
  REMARK    GeneRIF: The Multiple Roles of the Cytosolic Adapter Proteins ADAP,
            SKAP1 and SKAP2 for TCR/CD3 -Mediated Signaling Events.
            Review article
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3839)
  AUTHORS   Asazuma,N., Wilde,J.I., Berlanga,O., Leduc,M., Leo,A.,
            Schweighoffer,E., Tybulewicz,V., Bon,C., Liu,S.K., McGlade,C.J.,
            Schraven,B. and Watson,S.P.
  TITLE     Interaction of linker for activation of T cells with multiple
            adapter proteins in platelets activated by the glycoprotein
            VI-selective ligand, convulxin
  JOURNAL   J Biol Chem 275 (43), 33427-33434 (2000)
   PUBMED   10942756
REFERENCE   6  (bases 1 to 3839)
  AUTHORS   Wang,X. and Stollar,B.D.
  TITLE     Immunoglobulin VH gene expression in human aging
  JOURNAL   Clin Immunol 93 (2), 132-142 (1999)
   PUBMED   10527689
  REMARK    Erratum:[Clin Immunol 2000 Mar;94(3):219]
REFERENCE   7  (bases 1 to 3839)
  AUTHORS   Timms,J.F., Swanson,K.D., Marie-Cardine,A., Raab,M., Rudd,C.E.,
            Schraven,B. and Neel,B.G.
  TITLE     SHPS-1 is a scaffold for assembling distinct adhesion-regulated
            multi-protein complexes in macrophages
  JOURNAL   Curr Biol 9 (16), 927-930 (1999)
   PUBMED   10469599
REFERENCE   8  (bases 1 to 3839)
  AUTHORS   Kouroku,Y., Soyama,A., Fujita,E., Urase,K., Tsukahara,T. and
            Momoi,T.
  TITLE     RA70 is a src kinase-associated protein expressed ubiquitously
  JOURNAL   Biochem Biophys Res Commun 252 (3), 738-742 (1998)
   PUBMED   9837776
REFERENCE   9  (bases 1 to 3839)
  AUTHORS   Marie-Cardine,A., Verhagen,A.M., Eckerskorn,C. and Schraven,B.
  TITLE     SKAP-HOM, a novel adaptor protein homologous to the FYN-associated
            protein SKAP55
  JOURNAL   FEBS Lett 435 (1), 55-60 (1998)
   PUBMED   9755858
REFERENCE   10 (bases 1 to 3839)
  AUTHORS   Liu,J., Kang,H., Raab,M., da Silva,A.J., Kraeft,S.K. and Rudd,C.E.
  TITLE     FYB (FYN binding protein) serves as a binding partner for lymphoid
            protein and FYN kinase substrate SKAP55 and a SKAP55-related
            protein in T cells
  JOURNAL   Proc Natl Acad Sci U S A 95 (15), 8779-8784 (1998)
   PUBMED   9671755
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from HY044523.1, AK313209.1,
            BC002893.2, BC036044.1 and BQ433993.1.
            
            On Nov 23, 2018 this sequence version replaced NM_003930.4.
            
            Summary: The protein encoded by this gene shares homology with Src
            kinase-associated phosphoprotein 1, and is a substrate of Src
            family kinases. It is an adaptor protein that is thought to play an
            essential role in the Src signaling pathway, and in regulating
            proper activation of the immune system. This protein contains an
            amino terminal coiled-coil domain for self-dimerization, a
            plecskstrin homology (PH) domain required for interactions with
            lipids at the membrane, and a Src homology (SH3) domain at the
            carboxy terminus. Some reports indicate that this protein inhibits
            actin polymerization through interactions with actin assembly
            factors, and might negatively regulate the invasiveness of tumors
            by modulating actin assembly. Alternative splicing results in
            multiple transcript variants encoding different isoforms. [provided
            by RefSeq, Jan 2015].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC036044.1, SRR1803614.275728.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000345317.7/ ENSP00000005587.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1                 HY044523.1         155-155
            2-1241              AK313209.1         1-1240
            1242-2244           BC002893.2         1239-2241
            2245-2868           BC036044.1         2247-2870
            2869-2869           BQ433993.1         347-347
            2870-3839           BC036044.1         2872-3841
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7p15.2"
     gene            1..3839
                     /gene="SKAP2"
                     /gene_synonym="PRAP; RA70; SAPS; SCAP2; SKAP-HOM; SKAP55R"
                     /note="src kinase associated phosphoprotein 2"
                     /db_xref="GeneID:8935"
                     /db_xref="HGNC:HGNC:15687"
                     /db_xref="MIM:605215"
     exon            1..228
                     /gene="SKAP2"
                     /gene_synonym="PRAP; RA70; SAPS; SCAP2; SKAP-HOM; SKAP55R"
                     /inference="alignment:Splign:2.1.0"
     CDS             162..1241
                     /gene="SKAP2"
                     /gene_synonym="PRAP; RA70; SAPS; SCAP2; SKAP-HOM; SKAP55R"
                     /note="isoform 1 is encoded by transcript variant 1;
                     Fyn-associated phosphoprotein SKAP55 homologue; src
                     kinase-associated phosphoprotein of 55-related protein;
                     Pyk2/RAFTK-associated protein; SKAP-55HOM; retinoic
                     acid-induced protein 70; src family-associated
                     phosphoprotein 2; src-associated adapter protein with PH
                     and SH3 domains"
                     /codon_start=1
                     /product="src kinase-associated phosphoprotein 2 isoform
                     1"
                     /protein_id="NP_003921.2"
                     /db_xref="CCDS:CCDS5400.1"
                     /db_xref="GeneID:8935"
                     /db_xref="HGNC:HGNC:15687"
                     /db_xref="MIM:605215"
                     /translation="MPNPSSTSSPYPLPEEIRNLLADVETFVADILKGENLSKKAKEK
                     RESLIKKIKDVKSIYLQEFQDKGDAEDGEEYDDPFAGPPDTISLASERYDKDDEAPSD
                     GAQFPPIAAQDLPFVLKAGYLEKRRKDHSFLGFEWQKRWCALSKTVFYYYGSDKDKQQ
                     KGEFAIDGYSVRMNNTLRKDGKKDCCFEISAPDKRIYQFTAASPKDAEEWVQQLKFVL
                     QDMESDIIPEDYDERGELYDDVDHPLPISNPLTSSQPIDDEIYEELPEEEEDSAPVKV
                     EEQRKMSQDSVHHTSGDKSTDYANFYQGLWDCTGAFSDELSFKRGDVIYILSKEYNRY
                     GWWVGEMKGAIGLVPKAYIMEMYDI"
     exon            229..334
                     /gene="SKAP2"
                     /gene_synonym="PRAP; RA70; SAPS; SCAP2; SKAP-HOM; SKAP55R"
                     /inference="alignment:Splign:2.1.0"
     exon            335..360
                     /gene="SKAP2"
                     /gene_synonym="PRAP; RA70; SAPS; SCAP2; SKAP-HOM; SKAP55R"
                     /inference="alignment:Splign:2.1.0"
     exon            361..468
                     /gene="SKAP2"
                     /gene_synonym="PRAP; RA70; SAPS; SCAP2; SKAP-HOM; SKAP55R"
                     /inference="alignment:Splign:2.1.0"
     exon            469..546
                     /gene="SKAP2"
                     /gene_synonym="PRAP; RA70; SAPS; SCAP2; SKAP-HOM; SKAP55R"
                     /inference="alignment:Splign:2.1.0"
     exon            547..630
                     /gene="SKAP2"
                     /gene_synonym="PRAP; RA70; SAPS; SCAP2; SKAP-HOM; SKAP55R"
                     /inference="alignment:Splign:2.1.0"
     exon            631..755
                     /gene="SKAP2"
                     /gene_synonym="PRAP; RA70; SAPS; SCAP2; SKAP-HOM; SKAP55R"
                     /inference="alignment:Splign:2.1.0"
     exon            756..819
                     /gene="SKAP2"
                     /gene_synonym="PRAP; RA70; SAPS; SCAP2; SKAP-HOM; SKAP55R"
                     /inference="alignment:Splign:2.1.0"
     exon            820..957
                     /gene="SKAP2"
                     /gene_synonym="PRAP; RA70; SAPS; SCAP2; SKAP-HOM; SKAP55R"
                     /inference="alignment:Splign:2.1.0"
     exon            958..1035
                     /gene="SKAP2"
                     /gene_synonym="PRAP; RA70; SAPS; SCAP2; SKAP-HOM; SKAP55R"
                     /inference="alignment:Splign:2.1.0"
     exon            1036..1148
                     /gene="SKAP2"
                     /gene_synonym="PRAP; RA70; SAPS; SCAP2; SKAP-HOM; SKAP55R"
                     /inference="alignment:Splign:2.1.0"
     exon            1149..1250
                     /gene="SKAP2"
                     /gene_synonym="PRAP; RA70; SAPS; SCAP2; SKAP-HOM; SKAP55R"
                     /inference="alignment:Splign:2.1.0"
     exon            1251..3839
                     /gene="SKAP2"
                     /gene_synonym="PRAP; RA70; SAPS; SCAP2; SKAP-HOM; SKAP55R"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3817..3822
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SKAP2"
                     /gene_synonym="PRAP; RA70; SAPS; SCAP2; SKAP-HOM; SKAP55R"
                     /note="hexamer: AATAAA"
     polyA_site      3839
                     /gene="SKAP2"
                     /gene_synonym="PRAP; RA70; SAPS; SCAP2; SKAP-HOM; SKAP55R"
                     /note="major polyA site"
ORIGIN      
        1 gacttccttg ttgtgagccc cggcccggca gtgtcccgac tcgtagcccc gctgttctta
       61 atccgggccg ctagcctgag tctaggtcgc agccgcagcc ccaccccgtc ggtcaccttt
      121 tcagcgcagg tcctttcccc gcacgccctg cgctccctaa catgcccaac cccagcagca
      181 cctcctctcc ctaccccctc cctgaggaaa ttaggaacct gttggcagat gttgaaacat
      241 ttgtagcaga tatactgaaa ggagaaaatt tatccaagaa agcaaaggaa aagagagaat
      301 cccttattaa gaagataaaa gatgtaaagt ctatctatct tcaggaattt caagacaaag
      361 gtgatgcaga agatggggaa gaatatgatg acccttttgc tgggcctcca gacactattt
      421 cattagcctc agaacgatat gataaagacg atgaagcccc ctctgatgga gcccagtttc
      481 ctccaattgc agcacaagac cttccttttg ttctaaaggc tggctacctt gaaaaacgca
      541 gaaaagatca cagctttctg ggatttgaat ggcagaaacg gtggtgtgct ctcagtaaaa
      601 cggtattcta ttattatgga agtgataaag acaaacaaca gaaaggtgaa tttgcaatag
      661 atggctacag tgtcagaatg aataacactc taagaaagga tggaaagaaa gattgctgtt
      721 ttgaaatctc tgctcctgat aaacgtatat atcagtttac agcagcttct cccaaagatg
      781 ctgaagaatg ggtacagcag ctgaaatttg tattgcaaga tatggaatct gatattattc
      841 ctgaggatta tgatgagaga ggagaattat atgatgatgt tgatcatcct ctaccaataa
      901 gcaatccact aacaagcagt caaccaatag atgatgaaat ttatgaagaa cttccagaag
      961 aagaagagga cagtgctcca gtgaaagtgg aagaacaaag gaagatgagt caggatagtg
     1021 tccatcacac ctcaggggat aagagcactg attatgctaa tttttaccag ggattgtggg
     1081 attgtactgg agctttttct gatgagttgt catttaagcg tggtgatgtg atttacattc
     1141 ttagcaagga atacaataga tatggctggt gggtaggaga aatgaaggga gccattggct
     1201 tggtgcctaa agcctacata atggagatgt atgatatttg agagtcctgg aaaaggaaaa
     1261 ttcttctgct tgtctgcaaa tgctttggat ttagaagcgt catgaaagca cgagtgacag
     1321 ctcctaacct ctccttgttt tattaaacat tacttatctt tgactgttat tttatgcagt
     1381 cgctcattaa aatattcctc tgatgtgaaa ttaaatgaag gatattaatg taaattagat
     1441 gcaaccagtt aagttatacc tgttgctatt ttgcaaagaa ataattatag tttttattta
     1501 cccatttgat ttgtgtgaag aattcatcac tattttatac gtaacatata gtctactata
     1561 gcatagtatg ctactattgc tacttctggt gtgatttgta atgtttctta atcattggac
     1621 atcaattatt tttagagagt aatgtataat ttcatagcat tttaaattta gtgtatcatg
     1681 cgagtttttt ttggtagatg ctgaagaatg tggttgctaa acaaagaatg ctaaagaatg
     1741 ttcaaacttt atagataact ttattgttat tatttatttt cacacattta attcctatta
     1801 agtacagccg caagaaagaa aaaatgatga agttgcaaat ggcagtgtgc tgtcacctgc
     1861 aacagaagtg cgtaccagaa gtatgactca tgcaaagcat tttaccgtac aaatatcctg
     1921 gtgcgatgat gggcctggca acattttcta ctgtactttt gtttaaattt aatgaaacaa
     1981 aaaattccta agaaatacca ccctaccact aacaaaatgg tataaagaat ctcccagcca
     2041 ggccaacatg gtgaaaccct gtctctacta aaaattagcc aagtgtggta atgttcacct
     2101 gcagtcccag ctacttggga ggccaaggca cgagaattgc ttgaacaagg taggcagagg
     2161 ttgcagtgag ccaagatcgc gccactgcac tccagcctgg gcgacagagt gagactccat
     2221 ctcagaaaaa aaaaaaaaaa aaaacagaat ctcagtgtat tctcaaagta aaaaggcata
     2281 accaagcact ctcttatctt gccttattgc tatactattt acatcccact gcagaacagc
     2341 agatttgagg cttttctata tacttctcag agcactgaaa agaaaggaag ggtttgcaga
     2401 ggagagtgta gaaatcccag tggtagcatg taccaacagg tgagtagaaa ggtagtgtta
     2461 gcctgacatt tgagttatac tctgtgctgc ctgagcaaga tttgtagaat catataatta
     2521 ccttttcatg tatatttgaa atcagaggtg tttaaaatac ctatgagata ccaatgtagc
     2581 cttaacatat gtcaaaatgc attgctggtt agataattat ttggactaca cataaactcc
     2641 taatattgaa tcattaccta tcaggtatta tctttatgga acttttcaat atctttgctt
     2701 tataaagatt ctaaacatgt atctgagctg gtaatatttt aaaatctcca ttatttgtgt
     2761 aaaactgttt ataagcagtg tttgagaggg tctgctttac cattaccccc tcaatatcat
     2821 gatcatccaa tctcaaatgt gaaaaaaaaa aagaaatttg attttaggga ttgtgagtaa
     2881 acaagtttat atagagagac attgtgaagt taaagttttc agaagttaca tttgtgcagt
     2941 tcttaccttt tcctcatata gtgccattga aatagactga aattatcttg gcaaaagtta
     3001 gacaaccaaa gacgacttta gtggactggt tttcaaaact tgagcagctg aaaagcaaaa
     3061 gccgttgttt cccatgacaa tgtagccttt gtggatttgg gtttgtgctt tgggttgaaa
     3121 agaagttttt agtcctaggc cagtagatgg cagcagcttt tcattgcaga caaaacctct
     3181 tgaaaccctt cccccatggc acaaactcgc ccatgatgga aagcatctag atttctgcct
     3241 ccttttacag ttaatccagg agagggagtc ctttgccaac tgatgaccaa cagttccaag
     3301 ccagatagtc tcgtgaacag tgacaataca gaaataaggt gttatttctg ttcagatctc
     3361 caccggcctt tgttctttta aaacttgaat ataggtggga gacataagaa aggaaagaaa
     3421 agacttaaaa ctggagtgac aggacaaata atcattactt tcaattcatg actgctttat
     3481 attcatttga tgaaatcatt tgtatacaaa ccagggagag ttttctttac acccttgaca
     3541 atatatcaca tactcttcaa gatcataata atatcattaa tataaattta aacaacatgg
     3601 cttgttagaa aatatgctaa ttgctatggt ctcattatgt ttgcttagct tttatttgtt
     3661 tttctgtgaa cagttagaga gctaattttt ttcaaaggtg attgtaagtc atattttata
     3721 tagcattttg cttgattatt tgctctgtac tgaatttgta ctctattgcc attagatctt
     3781 acaataatgt tccactctgc aaatttttaa ggttcaaata aagtttaatt gtttgcaaa
//
