LOCUS       NM_003726               1556 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens src kinase associated phosphoprotein 1 (SKAP1),
            transcript variant 1, mRNA.
ACCESSION   NM_003726
VERSION     NM_003726.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Zhu,L., Yu,Q., Li,Y., Zhang,M., Peng,Z., Wang,S., Quan,Z. and
            Gao,D.
  TITLE     SKAP1 Is a Novel Biomarker and Therapeutic Target for Gastric
            Cancer: Evidence from Expression, Functional, and Bioinformatic
            Analyses
  JOURNAL   Int J Mol Sci 24 (14), 11870 (2023)
   PUBMED   37511629
  REMARK    GeneRIF: SKAP1 Is a Novel Biomarker and Therapeutic Target for
            Gastric Cancer: Evidence from Expression, Functional, and
            Bioinformatic Analyses.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Canson,D.M., O'Mara,T.A., Spurdle,A.B. and Glubb,D.M.
  TITLE     Splicing annotation of endometrial cancer GWAS risk loci reveals
            potentially causal variants and supports a role for NF1 and SKAP1
            as susceptibility genes
  JOURNAL   HGG Adv 4 (2), 100185 (2023)
   PUBMED   36908940
  REMARK    GeneRIF: Splicing annotation of endometrial cancer GWAS risk loci
            reveals potentially causal variants and supports a role for NF1 and
            SKAP1 as susceptibility genes.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1556)
  AUTHORS   Pu,Y., Wei,J., Wu,Y., Zhao,K., Wu,Y., Wu,S., Yang,X. and Xing,C.
  TITLE     THUMPD3-AS1 facilitates cell growth and aggressiveness by the
            miR-218-5p/SKAP1 axis in colorectal cancer
  JOURNAL   Cell Biochem Biophys 80 (3), 483-494 (2022)
   PUBMED   35538197
  REMARK    GeneRIF: THUMPD3-AS1 facilitates cell growth and aggressiveness by
            the miR-218-5p/SKAP1 axis in colorectal cancer.
            Erratum:[Cell Biochem Biophys. 2024 Mar;82(1):315. doi:
            10.1007/s12013-023-01199-0.. PMID: 38010601]
REFERENCE   4  (bases 1 to 1556)
  AUTHORS   Dadwal,N., Mix,C., Reinhold,A., Witte,A., Freund,C., Schraven,B.
            and Kliche,S.
  TITLE     The Multiple Roles of the Cytosolic Adapter Proteins ADAP, SKAP1
            and SKAP2 for TCR/CD3 -Mediated Signaling Events
  JOURNAL   Front Immunol 12, 703534 (2021)
   PUBMED   34295339
  REMARK    GeneRIF: The Multiple Roles of the Cytosolic Adapter Proteins ADAP,
            SKAP1 and SKAP2 for TCR/CD3 -Mediated Signaling Events.
            Review article
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1556)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   6  (bases 1 to 1556)
  AUTHORS   Kouroku,Y., Soyama,A., Fujita,E., Urase,K., Tsukahara,T. and
            Momoi,T.
  TITLE     RA70 is a src kinase-associated protein expressed ubiquitously
  JOURNAL   Biochem Biophys Res Commun 252 (3), 738-742 (1998)
   PUBMED   9837776
REFERENCE   7  (bases 1 to 1556)
  AUTHORS   Marie-Cardine,A., Hendricks-Taylor,L.R., Boerth,N.J., Zhao,H.,
            Schraven,B. and Koretzky,G.A.
  TITLE     Molecular interaction between the Fyn-associated protein SKAP55 and
            the SLP-76-associated phosphoprotein SLAP-130
  JOURNAL   J Biol Chem 273 (40), 25789-25795 (1998)
   PUBMED   9748251
REFERENCE   8  (bases 1 to 1556)
  AUTHORS   Liu,J., Kang,H., Raab,M., da Silva,A.J., Kraeft,S.K. and Rudd,C.E.
  TITLE     FYB (FYN binding protein) serves as a binding partner for lymphoid
            protein and FYN kinase substrate SKAP55 and a SKAP55-related
            protein in T cells
  JOURNAL   Proc Natl Acad Sci U S A 95 (15), 8779-8784 (1998)
   PUBMED   9671755
REFERENCE   9  (bases 1 to 1556)
  AUTHORS   Marie-Cardine,A., Bruyns,E., Eckerskorn,C., Kirchgessner,H.,
            Meuer,S.C. and Schraven,B.
  TITLE     Molecular cloning of SKAP55, a novel protein that associates with
            the protein tyrosine kinase p59fyn in human T-lymphocytes
  JOURNAL   J Biol Chem 272 (26), 16077-16080 (1997)
   PUBMED   9195899
REFERENCE   10 (bases 1 to 1556)
  AUTHORS   Schraven,B., Marie-Cardine,A. and Koretzky,G.
  TITLE     Molecular analysis of the fyn-complex: cloning of SKAP55 and
            SLAP-130, two novel adaptor proteins which associate with fyn and
            may participate in the regulation of T cell receptor-mediated
            signaling
  JOURNAL   Immunol Lett 57 (1-3), 165-169 (1997)
   PUBMED   9232446
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DR007155.1, Y11215.1 and
            BC047870.1.
            
            On Nov 22, 2018 this sequence version replaced NM_003726.3.
            
            Summary: This gene encodes a T cell adaptor protein, a class of
            intracellular molecules with modular domains capable of recruiting
            additional proteins but that exhibit no intrinsic enzymatic
            activity. The encoded protein contains a unique N-terminal region
            followed by a PH domain and C-terminal SH3 domain. Along with the
            adhesion and degranulation-promoting adaptor protein, the encoded
            protein plays a critical role in inside-out signaling by coupling
            T-cell antigen receptor stimulation to the activation of integrins.
            [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: Y11215.1, SRR18074968.2842179.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000336915.11/ ENSP00000338171.6
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-24                DR007155.1         24-47
            25-574              Y11215.1           1-550
            575-655             BC047870.1         565-645
            656-1205            Y11215.1           632-1181
            1206-1556           BC047870.1         1193-1543
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q21.32"
     gene            1..1556
                     /gene="SKAP1"
                     /gene_synonym="HEL-S-81p; SCAP1; SKAP55"
                     /note="src kinase associated phosphoprotein 1"
                     /db_xref="GeneID:8631"
                     /db_xref="HGNC:HGNC:15605"
                     /db_xref="MIM:604969"
     exon            1..140
                     /gene="SKAP1"
                     /gene_synonym="HEL-S-81p; SCAP1; SKAP55"
                     /inference="alignment:Splign:2.1.0"
     CDS             95..1174
                     /gene="SKAP1"
                     /gene_synonym="HEL-S-81p; SCAP1; SKAP55"
                     /note="isoform 1 is encoded by transcript variant 1; src
                     kinase-associated phosphoprotein of 55 kDa; pp55; SKAP-55;
                     src family-associated phosphoprotein 1; epididymis
                     secretory sperm binding protein Li 81p"
                     /codon_start=1
                     /product="src kinase-associated phosphoprotein 1 isoform
                     1"
                     /protein_id="NP_003717.3"
                     /db_xref="CCDS:CCDS32674.1"
                     /db_xref="GeneID:8631"
                     /db_xref="HGNC:HGNC:15605"
                     /db_xref="MIM:604969"
                     /translation="MQAAALPEEIRWLLEDAEEFLAEGLRNENLSAVARDHRDHILRG
                     FQQIKARYYWDFQPQGGDIGQDSSDDNHSGTLGLSLTSDAPFLSDYQDEGMEDIVKGA
                     QELDNVIKQGYLEKKSKDHSFFGSEWQKRWCVVSRGLFYYYANEKSKQPKGTFLIKGY
                     GVRMAPHLRRDSKKESCFELTSQDRRSYEFTATSPAEARDWVDQISFLLKDLSSLTIP
                     YEEDEEEEEKEETYDDIDGFDSPSCGSQCRPTILPGSVGIKEPTEEKEEEDIYEVLPD
                     EEHDLEEDESGTRRKGVDYASYYQGLWDCHGDQPDELSFQRGDLIRILSKEYNMYGWW
                     VGELNSLVGIVPKEYLTTAFEVEER"
     exon            141..246
                     /gene="SKAP1"
                     /gene_synonym="HEL-S-81p; SCAP1; SKAP55"
                     /inference="alignment:Splign:2.1.0"
     exon            247..272
                     /gene="SKAP1"
                     /gene_synonym="HEL-S-81p; SCAP1; SKAP55"
                     /inference="alignment:Splign:2.1.0"
     exon            273..374
                     /gene="SKAP1"
                     /gene_synonym="HEL-S-81p; SCAP1; SKAP55"
                     /inference="alignment:Splign:2.1.0"
     exon            375..452
                     /gene="SKAP1"
                     /gene_synonym="HEL-S-81p; SCAP1; SKAP55"
                     /inference="alignment:Splign:2.1.0"
     exon            453..536
                     /gene="SKAP1"
                     /gene_synonym="HEL-S-81p; SCAP1; SKAP55"
                     /inference="alignment:Splign:2.1.0"
     exon            537..661
                     /gene="SKAP1"
                     /gene_synonym="HEL-S-81p; SCAP1; SKAP55"
                     /inference="alignment:Splign:2.1.0"
     exon            662..725
                     /gene="SKAP1"
                     /gene_synonym="HEL-S-81p; SCAP1; SKAP55"
                     /inference="alignment:Splign:2.1.0"
     exon            726..920
                     /gene="SKAP1"
                     /gene_synonym="HEL-S-81p; SCAP1; SKAP55"
                     /inference="alignment:Splign:2.1.0"
     exon            921..971
                     /gene="SKAP1"
                     /gene_synonym="HEL-S-81p; SCAP1; SKAP55"
                     /inference="alignment:Splign:2.1.0"
     exon            972..1072
                     /gene="SKAP1"
                     /gene_synonym="HEL-S-81p; SCAP1; SKAP55"
                     /inference="alignment:Splign:2.1.0"
     exon            1073..1181
                     /gene="SKAP1"
                     /gene_synonym="HEL-S-81p; SCAP1; SKAP55"
                     /inference="alignment:Splign:2.1.0"
     exon            1182..1556
                     /gene="SKAP1"
                     /gene_synonym="HEL-S-81p; SCAP1; SKAP55"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1529..1534
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SKAP1"
                     /gene_synonym="HEL-S-81p; SCAP1; SKAP55"
                     /note="hexamer: AATAAA"
     polyA_site      1556
                     /gene="SKAP1"
                     /gene_synonym="HEL-S-81p; SCAP1; SKAP55"
                     /note="major polyA site"
ORIGIN      
        1 acaggtgcct cgcggtgcag ccgggtcgcc ttccagcccg tccgcctccc gaccagggcc
       61 cgcgccccgt cccgcctctc tcccgcccag ccaaatgcag gccgccgccc tccctgagga
      121 gatccgttgg ctcctggaag atgctgaaga gtttctggca gaaggtttgc ggaatgagaa
      181 cctcagcgct gttgcaaggg atcacagaga ccatattcta cggggctttc agcaaatcaa
      241 agccaggtac tattgggatt ttcagcccca agggggagac attggacagg acagctctga
      301 tgataatcac agcgggactc ttggcctgtc cctcacatcc gatgcaccct ttttgtcaga
      361 ttatcaggat gagggaatgg aagacatcgt aaaaggagct caagaacttg ataacgtaat
      421 caagcaagga tacttggaga agaaaagcaa agatcatagt ttctttggat cggagtggca
      481 gaagcgatgg tgtgttgtca gcagaggtct cttctactac tatgctaatg agaagagcaa
      541 gcagcccaaa gggaccttcc tcattaaggg ctacggtgta cggatggccc cccacctgcg
      601 aagagattcc aagaaagaat cctgctttga actgacctcc caggataggc gcagctatga
      661 gtttacagct actagtccag cagaagccag agactgggtg gatcaaataa gtttcttgtt
      721 aaaggatctg agctccttaa ccattccata tgaagaggat gaggaggaag aagaaaaaga
      781 agagacatat gatgatattg atggttttga ctccccaagt tgtggttccc agtgcagacc
      841 cactatcttg cctgggagtg tggggataaa agagcctaca gaggagaaag aagaagaaga
      901 tatttatgaa gtcttgccag atgaagagca tgatctagaa gaggatgaga gtggcactcg
      961 acgaaaagga gtagactatg ccagttacta ccagggccta tgggattgcc atggtgacca
     1021 gccagatgaa ctgtccttcc aacggggtga cctcatccgt attctgagca aggagtataa
     1081 catgtatggc tggtgggtgg gagaactgaa cagcctcgtt gggattgttc caaaggagta
     1141 tctcaccact gcctttgaag tggaagaaag atgaaaccca ggaaatatat tcttccctct
     1201 ctcctgcctt tatgaggaaa ctgatcatca aaagttccca ctccctactt ctgccacccc
     1261 accaacgcct tggactcctc tctttgctga agagacccaa gtctcttgac acctcagagt
     1321 gactgtaagc taccagtaag acaagtggga agaggcacgt tcatcaaacc tgttactaaa
     1381 ccagcctagt catagctcat ccccatctct aaatgtgtcc acacaaccac atctgccttt
     1441 tccacaagct tttcacaaag aaggtgagag agaaggaaac cttgggagga ggacattact
     1501 ggttgttctg gctggtttga aaagcacaaa taaacttggg atgtggttcc ttgcca
//
