LOCUS       NM_145061               2869 bp    mRNA    linear   PRI 01-JUL-2024
DEFINITION  Homo sapiens spindle and kinetochore associated complex subunit 3
            (SKA3), transcript variant 1, mRNA.
ACCESSION   NM_145061
VERSION     NM_145061.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Liang,G., Duan,C., He,J. and Shi,L.
  TITLE     Spindle and kinetochore-related complex subunit 3 has a protumour
            function in osteosarcoma by activating the Notch pathway
  JOURNAL   Toxicol Appl Pharmacol 483, 116826 (2024)
   PUBMED   38228236
  REMARK    GeneRIF: Spindle and kinetochore-related complex subunit 3 has a
            protumour function in osteosarcoma by activating the Notch pathway.
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Deng,J., Liao,S., Chen,C., Han,F., Lei,S., Lai,X., Ye,K., Han,Q.,
            E,F., Lu,C., Lai,M., Liu,F. and Zhang,H.
  TITLE     Specific intracellular retention of circSKA3 promotes colorectal
            cancer metastasis by attenuating ubiquitination and degradation of
            SLUG
  JOURNAL   Cell Death Dis 14 (11), 750 (2023)
   PUBMED   37973787
  REMARK    GeneRIF: Specific intracellular retention of circSKA3 promotes
            colorectal cancer metastasis by attenuating ubiquitination and
            degradation of SLUG.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2869)
  AUTHORS   Chen,Y., Xu,X., Wang,Y., Zhang,Y., Zhou,T., Jiang,W., Wang,Z.,
            Chang,J., Liu,S., Chen,R., Shan,J., Wang,J., Wang,Y., Li,C. and
            Li,X.
  TITLE     Hypoxia-induced SKA3 promoted cholangiocarcinoma progression and
            chemoresistance by enhancing fatty acid synthesis via the
            regulation of PAR-dependent HIF-1a deubiquitylation
  JOURNAL   J Exp Clin Cancer Res 42 (1), 265 (2023)
   PUBMED   37821935
  REMARK    GeneRIF: Hypoxia-induced SKA3 promoted cholangiocarcinoma
            progression and chemoresistance by enhancing fatty acid synthesis
            via the regulation of PAR-dependent HIF-1a deubiquitylation.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2869)
  AUTHORS   Ning,G., Lu,C., Chen,Y., Jiang,M., Si,P. and Zhang,R.
  TITLE     Transcription factor ZEB1 regulates PLK1-mediated SKA3
            phosphorylation to promote lung cancer cell proliferation,
            migration and cell cycle
  JOURNAL   Anticancer Drugs 34 (7), 866-876 (2023)
   PUBMED   36728910
  REMARK    GeneRIF: Transcription factor ZEB1 regulates PLK1-mediated SKA3
            phosphorylation to promote lung cancer cell proliferation,
            migration and cell cycle.
REFERENCE   5  (bases 1 to 2869)
  AUTHORS   Wang,C., Liu,S., Zhang,X., Wang,Y., Guan,P., Bu,F., Wang,H.,
            Wang,D., Fan,Y., Hou,S. and Qiu,Z.
  TITLE     SKA3 is a prognostic biomarker and associated with immune
            infiltration in bladder cancer
  JOURNAL   Hereditas 159 (1), 20 (2022)
   PUBMED   35546682
  REMARK    GeneRIF: SKA3 is a prognostic biomarker and associated with immune
            infiltration in bladder cancer.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2869)
  AUTHORS   Raaijmakers,J.A., Tanenbaum,M.E., Maia,A.F. and Medema,R.H.
  TITLE     RAMA1 is a novel kinetochore protein involved in
            kinetochore-microtubule attachment
  JOURNAL   J Cell Sci 122 (Pt 14), 2436-2445 (2009)
   PUBMED   19549680
  REMARK    GeneRIF: RAMA1 is a novel kinetochore protein involved in
            kinetochore-microtubule attachment.
REFERENCE   7  (bases 1 to 2869)
  AUTHORS   Guimaraes,G.J. and Deluca,J.G.
  TITLE     Connecting with Ska, a key complex at the kinetochore-microtubule
            interface
  JOURNAL   EMBO J 28 (10), 1375-1377 (2009)
   PUBMED   19458608
REFERENCE   8  (bases 1 to 2869)
  AUTHORS   Theis,M., Slabicki,M., Junqueira,M., Paszkowski-Rogacz,M.,
            Sontheimer,J., Kittler,R., Heninger,A.K., Glatter,T., Kruusmaa,K.,
            Poser,I., Hyman,A.A., Pisabarro,M.T., Gstaiger,M., Aebersold,R.,
            Shevchenko,A. and Buchholz,F.
  TITLE     Comparative profiling identifies C13orf3 as a component of the Ska
            complex required for mammalian cell division
  JOURNAL   EMBO J 28 (10), 1453-1465 (2009)
   PUBMED   19387489
  REMARK    GeneRIF: These data identify C13orf3 (Ska3) as an important factor
            for metaphase to anaphase progression and highlight the potential
            of combined RNAi screening and protein localisation analyses.
REFERENCE   9  (bases 1 to 2869)
  AUTHORS   Gaitanos,T.N., Santamaria,A., Jeyaprakash,A.A., Wang,B., Conti,E.
            and Nigg,E.A.
  TITLE     Stable kinetochore-microtubule interactions depend on the Ska
            complex and its new component Ska3/C13Orf3
  JOURNAL   EMBO J 28 (10), 1442-1452 (2009)
   PUBMED   19360002
  REMARK    GeneRIF: C13Orf3 (now termed Ska3) localizes to the spindle and
            kinetochore throughout mitosis and its depletion markedly delays
            anaphase transition.
REFERENCE   10 (bases 1 to 2869)
  AUTHORS   Welburn,J.P., Grishchuk,E.L., Backer,C.B., Wilson-Kubalek,E.M.,
            Yates,J.R. 3rd and Cheeseman,I.M.
  TITLE     The human kinetochore Ska1 complex facilitates microtubule
            depolymerization-coupled motility
  JOURNAL   Dev Cell 16 (3), 374-385 (2009)
   PUBMED   19289083
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL158032.32 and AF361358.1.
            
            On Nov 23, 2018 this sequence version replaced NM_145061.5.
            
            Summary: This gene encodes a component of the spindle and
            kinetochore-associated protein complex that regulates microtubule
            attachment to the kinetochores during mitosis. The encoded protein
            localizes to the outer kinetechore and may be required for normal
            chromosome segregation and cell division. Alternative splicing
            results in multiple transcript variants. [provided by RefSeq, Oct
            2009].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF361358.1, SRR1803611.270606.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000314759.6/ ENSP00000319417.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-8                 AL158032.32        39409-39416         c
            9-1061              AF361358.1         1-1053
            1062-1161           AL158032.32        20819-20918         c
            1162-1376           AF361358.1         1154-1368
            1377-2702           AL158032.32        16626-17951         c
            2703-2869           AF361358.1         2694-2860
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="13"
                     /map="13q12.11"
     gene            1..2869
                     /gene="SKA3"
                     /gene_synonym="C13orf3; RAMA1"
                     /note="spindle and kinetochore associated complex subunit
                     3"
                     /db_xref="GeneID:221150"
                     /db_xref="HGNC:HGNC:20262"
                     /db_xref="MIM:619247"
     exon            1..178
                     /gene="SKA3"
                     /gene_synonym="C13orf3; RAMA1"
                     /inference="alignment:Splign:2.1.0"
     CDS             76..1314
                     /gene="SKA3"
                     /gene_synonym="C13orf3; RAMA1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     spindle and kinetochore-associated protein 3"
                     /codon_start=1
                     /product="spindle and kinetochore-associated protein 3
                     isoform 1"
                     /protein_id="NP_659498.4"
                     /db_xref="CCDS:CCDS31946.1"
                     /db_xref="GeneID:221150"
                     /db_xref="HGNC:HGNC:20262"
                     /db_xref="MIM:619247"
                     /translation="MDPIRSFCGKLRSLASTLDCETARLQRALDGEESDFEDYPMRIL
                     YDLHSEVQTLKDDVNILLDKARLENQEGIDFIKATKVLMEKNSMDIMKIREYFQKYGY
                     SPRVKKNSVHEQEAINSDPELSNCENFQKTDVKDDLSDPPVASSCISEKSPRSPQLSD
                     FGLERYIVSQVLPNPPQAVNNYKEEPVIVTPPTKQSLVKVLKTPKCALKMDDFECVTP
                     KLEHFGISEYTMCLNEDYTMGLKNARNNKSEEAIDTESRLNDNVFATPSPIIQQLEKS
                     DAEYTNSPLVPTFCTPGLKIPSTKNSIALVSTNYPLSKTNSSSNDLEVEDRTSLVLNS
                     DTCFENLTDPSSPTISSYENLLRTPTPPEVTKIPEDILQLLSKYNSNLATPIAIKAVP
                     PSKRFLKHGQNIRDVSNKEN"
     exon            179..240
                     /gene="SKA3"
                     /gene_synonym="C13orf3; RAMA1"
                     /inference="alignment:Splign:2.1.0"
     exon            241..406
                     /gene="SKA3"
                     /gene_synonym="C13orf3; RAMA1"
                     /inference="alignment:Splign:2.1.0"
     exon            407..818
                     /gene="SKA3"
                     /gene_synonym="C13orf3; RAMA1"
                     /inference="alignment:Splign:2.1.0"
     exon            819..904
                     /gene="SKA3"
                     /gene_synonym="C13orf3; RAMA1"
                     /inference="alignment:Splign:2.1.0"
     exon            905..990
                     /gene="SKA3"
                     /gene_synonym="C13orf3; RAMA1"
                     /inference="alignment:Splign:2.1.0"
     exon            991..1194
                     /gene="SKA3"
                     /gene_synonym="C13orf3; RAMA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1195..1313
                     /gene="SKA3"
                     /gene_synonym="C13orf3; RAMA1"
                     /inference="alignment:Splign:2.1.0"
     exon            1314..2869
                     /gene="SKA3"
                     /gene_synonym="C13orf3; RAMA1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2526..2531
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SKA3"
                     /gene_synonym="C13orf3; RAMA1"
                     /note="hexamer: AATATA"
     polyA_site      2544
                     /gene="SKA3"
                     /gene_synonym="C13orf3; RAMA1"
                     /note="major polyA site"
     regulatory      2846..2851
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SKA3"
                     /gene_synonym="C13orf3; RAMA1"
                     /note="hexamer: AATAAA"
     polyA_site      2869
                     /gene="SKA3"
                     /gene_synonym="C13orf3; RAMA1"
                     /note="major polyA site"
ORIGIN      
        1 aaactagtgg cgggaggctg tgagctgagc ggtggggtct gcgtacgcct ggagtccttc
       61 cccgctgtgc tcagcatgga ccctatccgg agcttctgcg ggaagctgcg gtctctggcc
      121 agcacgctgg actgcgagac ggcccggctg cagcgagcgc tggacggaga ggaaagcgac
      181 tttgaagatt atccaatgag aattttatat gaccttcatt cagaagttca gactctaaag
      241 gatgatgtta atattcttct tgataaagca agattggaaa atcaagaagg cattgatttc
      301 ataaaggcaa caaaagtact aatggaaaaa aattcaatgg atattatgaa aataagagag
      361 tatttccaga agtatggata tagtccacgt gtcaagaaaa attcagtaca cgagcaagaa
      421 gccattaact ctgacccaga gttgtctaat tgtgaaaatt ttcagaagac tgatgtgaaa
      481 gatgatctgt ctgatcctcc tgttgcaagc agttgtattt ctgagaagtc tccacgtagt
      541 ccacaacttt cagattttgg acttgagcgg tacatcgtat cccaagttct accaaaccct
      601 ccacaggcag tgaacaacta taaggaagag cccgtaattg taaccccacc taccaaacaa
      661 tcactagtaa aagtactaaa aactccaaaa tgtgcactaa aaatggatga ttttgagtgt
      721 gtaactccta aattagaaca ctttggtatc tctgaatata ctatgtgttt aaatgaagat
      781 tacacaatgg gacttaaaaa tgcgaggaat aataaaagtg aggaggccat agatacagaa
      841 tccaggctca atgataatgt ttttgccact cccagcccca tcatccagca gttggaaaaa
      901 agtgatgccg aatataccaa ctctcctttg gtacctacat tctgtactcc tggtttgaaa
      961 attccatcta caaagaacag catagctttg gtatccacaa attacccatt atcaaaaaca
     1021 aatagttcat caaatgattt ggaagttgaa gatcgtactt cgttggtttt aaattcagac
     1081 acatgctttg agaatttaac agatccctct tcacctacga tttcttctta tgagaatctg
     1141 ctcagaacac ctacacctcc ggaagtaact aaaattccag aagatattct ccagctttta
     1201 tcaaaataca actcaaacct agctactcca atagcaatta aagcagtgcc acccagtaaa
     1261 aggttcctta aacatggaca gaacatccga gatgtcagca acaaagaaaa ctgaaattcc
     1321 agtggatcta tccaacacag aaactgaaca aaatgagatg aaagccgagc tggaccgatt
     1381 ttaacattca cattgccctg cctctgtccc cctttaaacg ttgacccatt ttaaagacaa
     1441 acatgaacat taacatcata atatgctttt tatgaagttt caataaggtt taaccttagt
     1501 cttgttgaca tgtagcccag tcattcactc tttaaggatt attagtgttt cattgatact
     1561 aaattaccca gcttaatcaa cagaatggtt taagtagtac caggaagtag gacaagtaat
     1621 ttcaaaaata taaaggtgtt tgctactcag atgaggccgc ccctgacctt ctggccagag
     1681 agacattgct gccagccagc tctgccttcc catcatctcc tttcaggacc gtcccacacc
     1741 ttttacttgc tcagtgctgt ctgaagatgc agttgctgtt tgcaaacaac aggaacacca
     1801 gttaaactaa ttaggaaaag agggagattt ccaggcctgg gtaactatat actgtgacca
     1861 ttggaggtag agacaggtct caacagttgg aaccaggaac tctgctgtca ggttgagagt
     1921 tttgtttctc ttccagcttt tcactgtgtg ggggtctttt ctcttatgtc agctctttct
     1981 atcacatggc agctgacctc tcacgctcca ctctgcagct tggacaccca gtagaccctg
     2041 aatttcactc tctctaaaag gttctgaggg ctcatcctgg gccaggggcc ctcctgtgca
     2101 ctgttagcta tggccacggg agcctccaga gctgcctggt agcttcaggt tgacctgctt
     2161 atcaggccta cgatccttct gatttaagta cagctggaaa gtattatcta attaagttca
     2221 tgatagtgct tttggagaac ttgtcaaatt acagccaatg agaaaataag gacctagcat
     2281 actgtggaga accattaaaa atttgagaag aaacaacaag tattatgtca acttacttca
     2341 aaggcgtagt tttgggaatt tgatgcagta aagattaccc tgttttatga ttgttccttg
     2401 aaagtcaaat gggggacctg tccattgtgc tctattaatc ttgtcagaaa actgtcacca
     2461 aaacaaaact tgagtttgtc cttgttctag gagttactgg gtagttgtaa gtattatttt
     2521 tattaaatat aatgtaaaat aaaatgttaa gatacttagt tttgtttttc aaagtaaagc
     2581 tgtagtcagc cttatgtatg ccattgactc tgaaatgtat accagccttt cactgtgtac
     2641 cgtgtgtata taaatccaca gaaccggatg agctgcttag ggagggaata tattcaaagt
     2701 gtaccaagga ccaaatcctg gagttctccc aactttagag gatggaaagg ggcagagtaa
     2761 tctagcaaag gagactgagg ccagtgaagt aggaagaaag tatttcaagg agagtgatga
     2821 ttctgtgaat attgctgaga attcaaataa aaagaggact gagaactga
//
