LOCUS       NM_145060               2849 bp    mRNA    linear   PRI 20-OCT-2024
DEFINITION  Homo sapiens spindle and kinetochore associated complex subunit 1
            (SKA1), transcript variant 2, mRNA.
ACCESSION   NM_145060
VERSION     NM_145060.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Zeng,W., Chen,Y., Liu,J., An,Z., Yan,H. and Sun,T.
  TITLE     Preliminary exploration of SKA1 expression in lung adenocarcinoma
            and its clinical significance
  JOURNAL   Acta Biochim Biophys Sin (Shanghai) 55 (12), 2008-2012 (2023)
   PUBMED   37814813
  REMARK    GeneRIF: Preliminary exploration of SKA1 expression in lung
            adenocarcinoma and its clinical significance.
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Zeng,F., Xu,Z. and Zhuang,P.
  TITLE     Integrated analysis of SKA1-related ceRNA network and SKA1
            immunoassays in HCC: A study based on bioinformatic
  JOURNAL   Medicine (Baltimore) 102 (38), e34826 (2023)
   PUBMED   37746945
  REMARK    GeneRIF: Integrated analysis of SKA1-related ceRNA network and SKA1
            immunoassays in HCC: A study based on bioinformatic.
REFERENCE   3  (bases 1 to 2849)
  AUTHORS   Tuo,H., Liu,R., Wang,Y., Yang,W. and Liu,Q.
  TITLE     Hypoxia-induced lncRNA MRVI1-AS1 accelerates hepatocellular
            carcinoma progression by recruiting RNA-binding protein CELF2 to
            stabilize SKA1 mRNA
  JOURNAL   World J Surg Oncol 21 (1), 111 (2023)
   PUBMED   36973749
  REMARK    GeneRIF: Hypoxia-induced lncRNA MRVI1-AS1 accelerates
            hepatocellular carcinoma progression by recruiting RNA-binding
            protein CELF2 to stabilize SKA1 mRNA.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2849)
  AUTHORS   Radhakrishnan,R.M., Kizhakkeduth,S.T., Nair,V.M., Ayyappan,S.,
            Lakshmi,R.B., Babu,N., Prasannajith,A., Umeda,K., Vijayan,V.,
            Kodera,N. and Manna,T.K.
  TITLE     Kinetochore-microtubule attachment in human cells is regulated by
            the interaction of a conserved motif of Ska1 with EB1
  JOURNAL   J Biol Chem 299 (2), 102853 (2023)
   PUBMED   36592928
  REMARK    GeneRIF: Kinetochore-microtubule attachment in human cells is
            regulated by the interaction of a conserved motif of Ska1 with EB1.
REFERENCE   5  (bases 1 to 2849)
  AUTHORS   Pu,Y., Han,J., Zhang,M., Liu,M., Abdusamat,G. and Liu,H.
  TITLE     SKA1 promotes tumor metastasis via SAFB-mediated transcription
            repression of DUSP6 in clear cell renal cell carcinoma
  JOURNAL   Aging (Albany NY) 14 (23), 9679-9698 (2022)
   PUBMED   36462498
  REMARK    GeneRIF: SKA1 promotes tumor metastasis via SAFB-mediated
            transcription repression of DUSP6 in clear cell renal cell
            carcinoma.
REFERENCE   6  (bases 1 to 2849)
  AUTHORS   Lampert,F. and Westermann,S.
  TITLE     A blueprint for kinetochores - new insights into the molecular
            mechanics of cell division
  JOURNAL   Nat Rev Mol Cell Biol 12 (7), 407-412 (2011)
   PUBMED   21633384
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 2849)
  AUTHORS   Gaitanos,T.N., Santamaria,A., Jeyaprakash,A.A., Wang,B., Conti,E.
            and Nigg,E.A.
  TITLE     Stable kinetochore-microtubule interactions depend on the Ska
            complex and its new component Ska3/C13Orf3
  JOURNAL   EMBO J 28 (10), 1442-1452 (2009)
   PUBMED   19360002
REFERENCE   8  (bases 1 to 2849)
  AUTHORS   Welburn,J.P., Grishchuk,E.L., Backer,C.B., Wilson-Kubalek,E.M.,
            Yates,J.R. 3rd and Cheeseman,I.M.
  TITLE     The human kinetochore Ska1 complex facilitates microtubule
            depolymerization-coupled motility
  JOURNAL   Dev Cell 16 (3), 374-385 (2009)
   PUBMED   19289083
  REMARK    GeneRIF: The full Ska1 complex forms assemblies on microtubules
            that can facilitate the processive movement of microspheres along
            depolymerizing microtubules.
REFERENCE   9  (bases 1 to 2849)
  AUTHORS   Hanisch,A., Sillje,H.H. and Nigg,E.A.
  TITLE     Timely anaphase onset requires a novel spindle and kinetochore
            complex comprising Ska1 and Ska2
  JOURNAL   EMBO J 25 (23), 5504-5515 (2006)
   PUBMED   17093495
  REMARK    GeneRIF: These data suggest that the Ska1/Ska2 complex plays a
            critical role in the maintenance of the metaphase plate and/or
            spindle checkpoint silencing.
REFERENCE   10 (bases 1 to 2849)
  AUTHORS   Sauer,G., Korner,R., Hanisch,A., Ries,A., Nigg,E.A. and Sillje,H.H.
  TITLE     Proteome analysis of the human mitotic spindle
  JOURNAL   Mol Cell Proteomics 4 (1), 35-43 (2005)
   PUBMED   15561729
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BU659790.1, BX956145.1, BC015706.1, AC105227.6 and BC015606.2.
            
            On Nov 22, 2018 this sequence version replaced NM_145060.3.
            
            Transcript Variant: This variant (2) uses a different splice site
            in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the
            same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.35554.1,
                                           SRR1163657.528964.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000285116.8/ ENSP00000285116.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-100               BU659790.1         25-124
            101-427             BX956145.1         73-399
            428-1046            BC015706.1         441-1059
            1047-2115           AC105227.6         34195-35263         c
            2116-2849           BC015606.2         1-734
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="18"
                     /map="18q21.1"
     gene            1..2849
                     /gene="SKA1"
                     /gene_synonym="C18orf24"
                     /note="spindle and kinetochore associated complex subunit
                     1"
                     /db_xref="GeneID:220134"
                     /db_xref="HGNC:HGNC:28109"
                     /db_xref="MIM:616673"
     exon            1..149
                     /gene="SKA1"
                     /gene_synonym="C18orf24"
                     /inference="alignment:Splign:2.1.0"
     exon            150..248
                     /gene="SKA1"
                     /gene_synonym="C18orf24"
                     /inference="alignment:Splign:2.1.0"
     CDS             161..928
                     /gene="SKA1"
                     /gene_synonym="C18orf24"
                     /note="spindle and KT (kinetochore) associated 1; spindle
                     and kinetochore-associated protein 1"
                     /codon_start=1
                     /product="SKA complex subunit 1"
                     /protein_id="NP_659497.1"
                     /db_xref="CCDS:CCDS11946.1"
                     /db_xref="GeneID:220134"
                     /db_xref="HGNC:HGNC:28109"
                     /db_xref="MIM:616673"
                     /translation="MASSDLEQLCSHVNEKIGNIKKTLSLRNCGQEPTLKTVLNKIGD
                     EIIVINELLNKLELEIQYQEQTNNSLKELCESLEEDYKDIEHLKENVPSHLPQVTVTQ
                     SCVKGSDLDPEEPIKVEEPEPVKKPPKEQRSIKEMPFITCDEFNGVPSYMKSRLTYNQ
                     INDVIKEINKAVISKYKILHQPKKSMNSVTRNLYHRFIDEETKDTKGRYFIVEADIKE
                     FTTLKADKKFHVLLNILRHCRRLSEVRGGGLTRYVIT"
     exon            249..373
                     /gene="SKA1"
                     /gene_synonym="C18orf24"
                     /inference="alignment:Splign:2.1.0"
     exon            374..471
                     /gene="SKA1"
                     /gene_synonym="C18orf24"
                     /inference="alignment:Splign:2.1.0"
     exon            472..609
                     /gene="SKA1"
                     /gene_synonym="C18orf24"
                     /inference="alignment:Splign:2.1.0"
     exon            610..779
                     /gene="SKA1"
                     /gene_synonym="C18orf24"
                     /inference="alignment:Splign:2.1.0"
     exon            780..2849
                     /gene="SKA1"
                     /gene_synonym="C18orf24"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2831..2836
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SKA1"
                     /gene_synonym="C18orf24"
                     /note="hexamer: AATAAA"
     polyA_site      2849
                     /gene="SKA1"
                     /gene_synonym="C18orf24"
                     /note="major polyA site"
ORIGIN      
        1 gtgcgcctgc gctgcgctag ggcgcggcgg gcggtttgaa ttttgcttac agagtcccgt
       61 ctcaccatcc tgggcttcca acggagactg cggtatccgc agctggagac ccagcggcga
      121 gtagcctttt gctcccggac ggacttgaga ggcttaaagg atggcctcgt cagatctgga
      181 acaattatgc tctcatgtta atgaaaagat tggcaatatt aagaaaacct tatcattaag
      241 aaactgtggc caggaaccta ccttgaaaac tgtattaaat aaaataggag atgagatcat
      301 tgtaataaat gaacttctaa ataaattgga attggaaatt cagtatcaag aacaaaccaa
      361 caattcactc aaggaactct gtgaatctct tgaagaagat tacaaagaca tagaacatct
      421 taaagaaaac gttccttccc atttgcctca agtaacagta acccagagct gtgttaaggg
      481 atcagatctt gatcctgaag aaccaatcaa agttgaagaa cctgaacccg taaagaagcc
      541 tcccaaagag caaagaagta ttaaggaaat gccatttata acttgtgatg agttcaatgg
      601 tgttccttcg tacatgaaat cccgcttaac ctataatcaa attaatgatg ttattaaaga
      661 aatcaacaag gcagtaatta gtaaatataa aatcctacat cagccaaaaa agtctatgaa
      721 ttctgtgacc agaaatctct atcacagatt tattgatgaa gaaacgaagg ataccaaagg
      781 tcgttatttt atagtggaag ctgacataaa ggagttcaca actttgaaag ctgacaagaa
      841 gtttcacgtg ttactgaata ttttacgaca ctgccggagg ctatcagagg tccgaggggg
      901 aggacttact cgttatgtta taacctgagt cccttgtgaa cttttgaaca taccaacagg
      961 gtatagagta tagaggctat ttctataatt ttcttatata taattttttt aacttttaat
     1021 cttttttgtt tccttttttt tttttttgag acaggatctt gctttgtcac ccaggggctt
     1081 gctttgtcac gcaggctaga gtgcagtggc gcaaacatgg ctcactgcag cctcaacctc
     1141 ccaggctcaa gtgatcctcc cacctcagcc ccctgaatgg ctgggactac aagcgtgcgc
     1201 caccatgcct ggctaatttt tgtatttttt ggagagatgg ggtttcacca tgttgcctag
     1261 gctggtcttg agctcctgag ctcaaacaat ccaccctcct cagcctccca aagtgctggg
     1321 attacaggct tgagccacca cacctgacct attcttgttt cttataaaaa taaaactttt
     1381 ttggataaag cttatttctt gtttttttct ttttcttttt tttttttttt gagacagtct
     1441 cgctctttca cccaggccgg actgcagcgg cgctatctcg gctcactgca aactctgcct
     1501 cccaggttca cgccatcctc ctgcctcagc ctcctgagta gctgggacta caggcacctg
     1561 ccaccacgcc cagctaattt tttgtatttt tagtagagat ggggtttcac cgtgttagcc
     1621 aggatggtct cgatctcctg acctcatgat ccgcccacct cggcctccca aagtgctggg
     1681 attacaggcg tgagccaccg cgcccggcca acttatatat tttcaagatt cacctcctct
     1741 caccaaatat ttaactacct gctgaatacg cctctgtact aggcacataa tggaactaaa
     1801 aaatgctcat gtccagtttt tgtgttgagt gaacaatgct gcagacccta ataagattgg
     1861 gtacagatcg gcatgcgcct gtagtcccag ctactcagga gaattgcttg aacctaggag
     1921 gtggaggttg cagtgagccg agatcgtgcc actgcactcc agtctgggca acagagcgag
     1981 actccatctc agaaaaaaag aaaaaaagac tgggtacaga tgtgatattg gaagaaaaag
     2041 atcaagctga tgaggttagg atacccaggc cctttggact taaagatcac tagtgtctaa
     2101 attccatcga tggcatttca gtctataggt aaacttcctg gaagctggat ttggagacag
     2161 tttatcatct gattattggg ctttcgtata ggtccttagg gagcagctta cctgaaatgc
     2221 atttagtgta caccagtctg taaacttcaa cctgtaatga aagtgtaata aatgtacatt
     2281 gagttgatgt gataatgtga tataataaga aatatatatt tgatcttcct atctagttcc
     2341 ttgttcagag ctcctaaaac ccttgtaatt tccaaagtga tggagtacat cttttgttct
     2401 agtatttggt ctttgacccc agttcctgac acaaagctcc taaattcctt taaatttccc
     2461 agtgatagga gaattttttg ttctaatgag gtcactcttg atgggcacct ggataactca
     2521 ggatgggggc tgctcacaaa gaccacatca tgattggaag tttcaaactt tcagtctccc
     2581 acctccagag aggggagagg ggctggagat ttgtgtcaat aatccatcag gcctatgtca
     2641 acaagacata atccgttaac tatggagttc agggagcttc agggttggca aacattttga
     2701 tgtgccagga aggtgacgca ctccagcttt atgaagtcag caagtcctgt gctcaggatg
     2761 cttctggacc ttgccccagg taccccttca tgtggctgtt gttcatctgt atcctttgta
     2821 gtagccttaa aataaactgt taaacacaa
//
