LOCUS       NP_659497                255 aa            linear   PRI 27-APR-2025
DEFINITION  SKA complex subunit 1 [Homo sapiens].
ACCESSION   NP_659497
VERSION     NP_659497.1
DBSOURCE    REFSEQ: accession NM_145060.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 255)
  AUTHORS   Zeng,W., Chen,Y., Liu,J., An,Z., Yan,H. and Sun,T.
  TITLE     Preliminary exploration of SKA1 expression in lung adenocarcinoma
            and its clinical significance
  JOURNAL   Acta Biochim Biophys Sin (Shanghai) 55 (12), 2008-2012 (2023)
   PUBMED   37814813
  REMARK    GeneRIF: Preliminary exploration of SKA1 expression in lung
            adenocarcinoma and its clinical significance.
REFERENCE   2  (residues 1 to 255)
  AUTHORS   Zeng,F., Xu,Z. and Zhuang,P.
  TITLE     Integrated analysis of SKA1-related ceRNA network and SKA1
            immunoassays in HCC: A study based on bioinformatic
  JOURNAL   Medicine (Baltimore) 102 (38), e34826 (2023)
   PUBMED   37746945
  REMARK    GeneRIF: Integrated analysis of SKA1-related ceRNA network and SKA1
            immunoassays in HCC: A study based on bioinformatic.
REFERENCE   3  (residues 1 to 255)
  AUTHORS   Tuo,H., Liu,R., Wang,Y., Yang,W. and Liu,Q.
  TITLE     Hypoxia-induced lncRNA MRVI1-AS1 accelerates hepatocellular
            carcinoma progression by recruiting RNA-binding protein CELF2 to
            stabilize SKA1 mRNA
  JOURNAL   World J Surg Oncol 21 (1), 111 (2023)
   PUBMED   36973749
  REMARK    GeneRIF: Hypoxia-induced lncRNA MRVI1-AS1 accelerates
            hepatocellular carcinoma progression by recruiting RNA-binding
            protein CELF2 to stabilize SKA1 mRNA.
            Publication Status: Online-Only
REFERENCE   4  (residues 1 to 255)
  AUTHORS   Radhakrishnan,R.M., Kizhakkeduth,S.T., Nair,V.M., Ayyappan,S.,
            Lakshmi,R.B., Babu,N., Prasannajith,A., Umeda,K., Vijayan,V.,
            Kodera,N. and Manna,T.K.
  TITLE     Kinetochore-microtubule attachment in human cells is regulated by
            the interaction of a conserved motif of Ska1 with EB1
  JOURNAL   J Biol Chem 299 (2), 102853 (2023)
   PUBMED   36592928
  REMARK    GeneRIF: Kinetochore-microtubule attachment in human cells is
            regulated by the interaction of a conserved motif of Ska1 with EB1.
REFERENCE   5  (residues 1 to 255)
  AUTHORS   Pu,Y., Han,J., Zhang,M., Liu,M., Abdusamat,G. and Liu,H.
  TITLE     SKA1 promotes tumor metastasis via SAFB-mediated transcription
            repression of DUSP6 in clear cell renal cell carcinoma
  JOURNAL   Aging (Albany NY) 14 (23), 9679-9698 (2022)
   PUBMED   36462498
  REMARK    GeneRIF: SKA1 promotes tumor metastasis via SAFB-mediated
            transcription repression of DUSP6 in clear cell renal cell
            carcinoma.
REFERENCE   6  (residues 1 to 255)
  AUTHORS   Lampert,F. and Westermann,S.
  TITLE     A blueprint for kinetochores - new insights into the molecular
            mechanics of cell division
  JOURNAL   Nat Rev Mol Cell Biol 12 (7), 407-412 (2011)
   PUBMED   21633384
  REMARK    Publication Status: Online-Only
REFERENCE   7  (residues 1 to 255)
  AUTHORS   Gaitanos,T.N., Santamaria,A., Jeyaprakash,A.A., Wang,B., Conti,E.
            and Nigg,E.A.
  TITLE     Stable kinetochore-microtubule interactions depend on the Ska
            complex and its new component Ska3/C13Orf3
  JOURNAL   EMBO J 28 (10), 1442-1452 (2009)
   PUBMED   19360002
REFERENCE   8  (residues 1 to 255)
  AUTHORS   Welburn,J.P., Grishchuk,E.L., Backer,C.B., Wilson-Kubalek,E.M.,
            Yates,J.R. 3rd and Cheeseman,I.M.
  TITLE     The human kinetochore Ska1 complex facilitates microtubule
            depolymerization-coupled motility
  JOURNAL   Dev Cell 16 (3), 374-385 (2009)
   PUBMED   19289083
  REMARK    GeneRIF: The full Ska1 complex forms assemblies on microtubules
            that can facilitate the processive movement of microspheres along
            depolymerizing microtubules.
REFERENCE   9  (residues 1 to 255)
  AUTHORS   Hanisch,A., Sillje,H.H. and Nigg,E.A.
  TITLE     Timely anaphase onset requires a novel spindle and kinetochore
            complex comprising Ska1 and Ska2
  JOURNAL   EMBO J 25 (23), 5504-5515 (2006)
   PUBMED   17093495
  REMARK    GeneRIF: These data suggest that the Ska1/Ska2 complex plays a
            critical role in the maintenance of the metaphase plate and/or
            spindle checkpoint silencing.
REFERENCE   10 (residues 1 to 255)
  AUTHORS   Sauer,G., Korner,R., Hanisch,A., Ries,A., Nigg,E.A. and Sillje,H.H.
  TITLE     Proteome analysis of the human mitotic spindle
  JOURNAL   Mol Cell Proteomics 4 (1), 35-43 (2005)
   PUBMED   15561729
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BU659790.1, BX956145.1, BC015706.1, AC105227.6 and BC015606.2.
            
            Transcript Variant: This variant (2) uses a different splice site
            in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the
            same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.35554.1,
                                           SRR1163657.528964.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000285116.8/ ENSP00000285116.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="18"
                     /map="18q21.1"
     Protein         1..255
                     /product="SKA complex subunit 1"
                     /note="spindle and KT (kinetochore) associated 1; spindle
                     and kinetochore-associated protein 1"
                     /calculated_mol_wt=29353
     Site            2
                     /site_type="acetylation"
                     /note="N-acetylalanine.
                     /evidence=ECO:0007744|PubMed:19413330; propagated from
                     UniProtKB/Swiss-Prot (Q96BD8.1)"
     Site            order(4..6,9)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:214021"
     Site            order(6,9..10,13,17,20..21,24,27..28,35,38..39,42,45..46,
                     48..49,52..53,55..56,59,62..63,66,70,73,77)
                     /site_type="other"
                     /note="SKA3 interface [polypeptide binding]"
                     /db_xref="CDD:214021"
     Site            order(12,16,19..20,23..24,26..27,30,42,46,49..50,53..54,
                     56..57,60,63,67,70..71,73..74,77)
                     /site_type="other"
                     /note="SKA2 interface [polypeptide binding]"
                     /db_xref="CDD:214021"
     Region          16..248
                     /region_name="SKA1"
                     /note="Spindle and kinetochore-associated protein 1;
                     pfam07160"
                     /db_xref="CDD:462107"
     Region          92..132
                     /region_name="Flexiple loop that anchors MAPRE1.
                     /evidence=ECO:0000269|PubMed:24413531,
                     ECO:0000269|PubMed:36592928"
                     /note="propagated from UniProtKB/Swiss-Prot (Q96BD8.1)"
     Region          93..96
                     /region_name="SXLP motif, mediates interaction with
                     MAPRE1, targeting to microtubule plus ends, stabilization
                     on kinetochores and is required for proper chromosome
                     alignment to the metaphase plate.
                     /evidence=ECO:0000269|PubMed:36592928"
                     /note="propagated from UniProtKB/Swiss-Prot (Q96BD8.1)"
     Region          106..131
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q96BD8.1)"
     Region          132..255
                     /region_name="Binds microtubules and protein phosphatase
                     PP1 subunit PPP1CA. /evidence=ECO:0000269|PubMed:22483620,
                     ECO:0000269|PubMed:23085020, ECO:0000269|PubMed:24413531,
                     ECO:0000269|PubMed:26981768, ECO:0000269|PubMed:27667719,
                     ECO:0000269|PubMed:29153323, ECO:0000269|PubMed:29487209"
                     /note="propagated from UniProtKB/Swiss-Prot (Q96BD8.1)"
     Site            157
                     /site_type="phosphorylation"
                     /note="Phosphothreonine, by AURKB.
                     /evidence=ECO:0000269|PubMed:22371557; propagated from
                     UniProtKB/Swiss-Prot (Q96BD8.1)"
     Site            242
                     /site_type="phosphorylation"
                     /note="Phosphoserine, by AURKB.
                     /evidence=ECO:0000269|PubMed:22371557; propagated from
                     UniProtKB/Swiss-Prot (Q96BD8.1)"
     CDS             1..255
                     /gene="SKA1"
                     /gene_synonym="C18orf24"
                     /coded_by="NM_145060.4:161..928"
                     /db_xref="CCDS:CCDS11946.1"
                     /db_xref="GeneID:220134"
                     /db_xref="HGNC:HGNC:28109"
                     /db_xref="MIM:616673"
ORIGIN      
        1 massdleqlc shvnekigni kktlslrncg qeptlktvln kigdeiivin ellnklelei
       61 qyqeqtnnsl kelcesleed ykdiehlken vpshlpqvtv tqscvkgsdl dpeepikvee
      121 pepvkkppke qrsikempfi tcdefngvps ymksrltynq indvikeink aviskykilh
      181 qpkksmnsvt rnlyhrfide etkdtkgryf iveadikeft tlkadkkfhv llnilrhcrr
      241 lsevrggglt ryvit
//
