LOCUS       NM_012239               2882 bp    mRNA    linear   PRI 15-DEC-2024
DEFINITION  Homo sapiens sirtuin 3 (SIRT3), transcript variant 1, mRNA; nuclear
            gene for mitochondrial product.
ACCESSION   NM_012239
VERSION     NM_012239.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Hu,H.J., Wang,X.H., Zhang,Z.Z., Ou,Y., Ning,Z.H., Yang,J.Y.,
            Huang,H., Tang,H.F. and Jiang,Z.S.
  TITLE     SIRT3 sulfhydration using hydrogen sulfide inhibited angiotensin
            II-induced atrial fibrosis and vulnerability to atrial fibrillation
            via suppression of the TGF-beta1/smad2/3 signalling pathway
  JOURNAL   Eur J Pharmacol 982, 176900 (2024)
   PUBMED   39168432
  REMARK    GeneRIF: SIRT3 sulfhydration using hydrogen sulfide inhibited
            angiotensin II-induced atrial fibrosis and vulnerability to atrial
            fibrillation via suppression of the TGF-beta1/smad2/3 signalling
            pathway.
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Zhu,C., Duan,Y., Dong,J., Jia,H., Zhang,L., Xing,A., Li,Z., Du,B.,
            Sun,Q., Huang,Y., Zhang,Z. and Pan,L.
  TITLE     Quantitative analysis of the lysine acetylome reveals the role of
            SIRT3-mediated HSP60 deacetylation in suppressing intracellular
            Mycobacterium tuberculosis survival
  JOURNAL   Microbiol Spectr 12 (8), e0074924 (2024)
   PUBMED   38916288
  REMARK    GeneRIF: Quantitative analysis of the lysine acetylome reveals the
            role of SIRT3-mediated HSP60 deacetylation in suppressing
            intracellular Mycobacterium tuberculosis survival.
REFERENCE   3  (bases 1 to 2882)
  AUTHORS   Yao,H., Zhao,H., Du,Y., Zhang,Y., Li,Y. and Zhu,H.
  TITLE     Sex-related differences in SIRT3-mediated mitochondrial dynamics in
            renal ischemia/reperfusion injury
  JOURNAL   Transl Res 270, 1-12 (2024)
   PUBMED   38556109
  REMARK    GeneRIF: Sex-related differences in SIRT3-mediated mitochondrial
            dynamics in renal ischemia/reperfusion injury.
REFERENCE   4  (bases 1 to 2882)
  AUTHORS   Zhang,L., Dai,Z., Shi,S., Yan,Z., Yang,J., Xue,W., He,Y., Mi,S.,
            Cheng,C., Wang,L., Li,N., Tan,W., Jiang,Z., Sun,H. and Li,S.
  TITLE     SIRT3 and SIRT4 double-genes remodeled the mitochondrial network to
            induce hepatocellular carcinoma cell line differentiation and
            suppress malignant phenotypes
  JOURNAL   Biochem Pharmacol 223, 116168 (2024)
   PUBMED   38548246
  REMARK    GeneRIF: SIRT3 and SIRT4 double-genes remodeled the mitochondrial
            network to induce hepatocellular carcinoma cell line
            differentiation and suppress malignant phenotypes.
REFERENCE   5  (bases 1 to 2882)
  AUTHORS   Ma,R., Gao,Q.Y., Chen,Z.T., Liao,G.H., Li,S.T., Cai,J.W., Luo,N.S.,
            Chen,H. and Zhang,H.F.
  TITLE     SIRT3 suppression resulting from the enhanced beta-catenin
            signaling drives glycolysis and promotes hypoxia-induced cell
            growth in hepatocellular carcinoma cells
  JOURNAL   Cell Cycle 23 (4), 435-447 (2024)
   PUBMED   38626328
  REMARK    GeneRIF: SIRT3 suppression resulting from the enhanced beta-catenin
            signaling drives glycolysis and promotes hypoxia-induced cell
            growth in hepatocellular carcinoma cells.
REFERENCE   6  (bases 1 to 2882)
  AUTHORS   Onyango,P., Celic,I., McCaffery,J.M., Boeke,J.D. and Feinberg,A.P.
  TITLE     SIRT3, a human SIR2 homologue, is an NAD-dependent deacetylase
            localized to mitochondria
  JOURNAL   Proc Natl Acad Sci U S A 99 (21), 13653-13658 (2002)
   PUBMED   12374852
  REMARK    GeneRIF: the role of SIRT3 in mitochondria involves protein
            deacetylation
REFERENCE   7  (bases 1 to 2882)
  AUTHORS   Schwer,B., North,B.J., Frye,R.A., Ott,M. and Verdin,E.
  TITLE     The human silent information regulator (Sir)2 homologue hSIRT3 is a
            mitochondrial nicotinamide adenine dinucleotide-dependent
            deacetylase
  JOURNAL   J Cell Biol 158 (4), 647-657 (2002)
   PUBMED   12186850
  REMARK    GeneRIF: hSIRT3 is a mitochondrial nicotinamide adenine
            dinucleotide-dependent deacetylase. hSIRT3 is proteolytically
            processed in the mitochondrial matrix to a 28-kD product.
REFERENCE   8  (bases 1 to 2882)
  AUTHORS   Yang,Y.H., Chen,Y.H., Zhang,C.Y., Nimmakayalu,M.A., Ward,D.C. and
            Weissman,S.
  TITLE     Cloning and characterization of two mouse genes with homology to
            the yeast Sir2 gene
  JOURNAL   Genomics 69 (3), 355-369 (2000)
   PUBMED   11056054
REFERENCE   9  (bases 1 to 2882)
  AUTHORS   Frye,R.A.
  TITLE     Phylogenetic classification of prokaryotic and eukaryotic Sir2-like
            proteins
  JOURNAL   Biochem Biophys Res Commun 273 (2), 793-798 (2000)
   PUBMED   10873683
REFERENCE   10 (bases 1 to 2882)
  AUTHORS   Frye,R.A.
  TITLE     Characterization of five human cDNAs with homology to the yeast
            SIR2 gene: Sir2-like proteins (sirtuins) metabolize NAD and may
            have protein ADP-ribosyltransferase activity
  JOURNAL   Biochem Biophys Res Commun 260 (1), 273-279 (1999)
   PUBMED   10381378
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL535769.3, BC001042.2,
            DR762907.1, AI091200.1, AC069287.7 and BM973763.1.
            
            On Nov 22, 2018 this sequence version replaced NM_012239.5.
            
            Summary: SIRT3 encodes a member of the sirtuin family of class III
            histone deacetylases, homologs to the yeast Sir2 protein. The
            encoded protein is found exclusively in mitochondria, where it can
            eliminate reactive oxygen species, inhibit apoptosis, and prevent
            the formation of cancer cells. SIRT3 has far-reaching effects on
            nuclear gene expression, cancer, cardiovascular disease,
            neuroprotection, aging, and metabolic control. [provided by RefSeq,
            May 2019].
            
            Transcript Variant: This variant (1) encodes isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR18074969.298331.1,
                                           SRR11853561.4359.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000382743.9/
                                                  ENSP00000372191.4
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-86                AL535769.3         2-87
            87-1770             BC001042.2         90-1773
            1771-2024           DR762907.1         505-758
            2025-2485           AI091200.1         16-476              c
            2486-2548           AC069287.7         44062-44124
            2549-2882           BM973763.1         18-351              c
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11p15.5"
     gene            1..2882
                     /gene="SIRT3"
                     /gene_synonym="SIR2L3"
                     /note="sirtuin 3"
                     /db_xref="GeneID:23410"
                     /db_xref="HGNC:HGNC:14931"
                     /db_xref="MIM:604481"
     exon            1..295
                     /gene="SIRT3"
                     /gene_synonym="SIR2L3"
                     /inference="alignment:Splign:2.1.0"
     CDS             15..1214
                     /gene="SIRT3"
                     /gene_synonym="SIR2L3"
                     /EC_number="2.3.1.286"
                     /note="isoform a is encoded by transcript variant 1;
                     sir2-like 3; silent mating type information regulation 2,
                     S.cerevisiae, homolog 3; mitochondrial nicotinamide
                     adenine dinucleotide-dependent deacetylase; sirtuin type
                     3; NAD-dependent deacetylase sirtuin-3, mitochondrial;
                     SIR2-like protein 3; regulatory protein SIR2 homolog 3;
                     NAD-dependent protein deacetylase sirtuin-3,
                     mitochondrial; NAD-dependent protein delactylase
                     sirtuin-3"
                     /codon_start=1
                     /product="NAD-dependent protein deacetylase sirtuin-3,
                     mitochondrial isoform a"
                     /protein_id="NP_036371.1"
                     /db_xref="CCDS:CCDS7691.1"
                     /db_xref="GeneID:23410"
                     /db_xref="HGNC:HGNC:14931"
                     /db_xref="MIM:604481"
                     /translation="MAFWGWRAAAALRLWGRVVERVEAGGGVGPFQACGCRLVLGGRD
                     DVSAGLRGSHGARGEPLDPARPLQRPPRPEVPRAFRRQPRAAAPSFFFSSIKGGRRSI
                     SFSVGASSVVGSGGSSDKGKLSLQDVAELIRARACQRVVVMVGAGISTPSGIPDFRSP
                     GSGLYSNLQQYDLPYPEAIFELPFFFHNPKPFFTLAKELYPGNYKPNVTHYFLRLLHD
                     KGLLLRLYTQNIDGLERVSGIPASKLVEAHGTFASATCTVCQRPFPGEDIRADVMADR
                     VPRCPVCTGVVKPDIVFFGEPLPQRFLLHVVDFPMADLLLILGTSLEVEPFASLTEAV
                     RSSVPRLLINRDLVGPLAWHPRSRDVAQLGDVVHGVESLVELLGWTEEMRDLVQRETG
                     KLDGPDK"
     exon            296..487
                     /gene="SIRT3"
                     /gene_synonym="SIR2L3"
                     /inference="alignment:Splign:2.1.0"
     exon            488..720
                     /gene="SIRT3"
                     /gene_synonym="SIR2L3"
                     /inference="alignment:Splign:2.1.0"
     exon            721..821
                     /gene="SIRT3"
                     /gene_synonym="SIR2L3"
                     /inference="alignment:Splign:2.1.0"
     exon            822..983
                     /gene="SIRT3"
                     /gene_synonym="SIR2L3"
                     /inference="alignment:Splign:2.1.0"
     exon            984..1193
                     /gene="SIRT3"
                     /gene_synonym="SIR2L3"
                     /inference="alignment:Splign:2.1.0"
     exon            1194..2882
                     /gene="SIRT3"
                     /gene_synonym="SIR2L3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1750..1755
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SIRT3"
                     /gene_synonym="SIR2L3"
                     /note="hexamer: AATACA"
     polyA_site      1774
                     /gene="SIRT3"
                     /gene_synonym="SIR2L3"
                     /note="major polyA site"
     regulatory      2463..2468
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SIRT3"
                     /gene_synonym="SIR2L3"
                     /note="hexamer: AATGAA"
     polyA_site      2485
                     /gene="SIRT3"
                     /gene_synonym="SIR2L3"
                     /note="major polyA site"
     regulatory      2861..2866
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SIRT3"
                     /gene_synonym="SIR2L3"
                     /note="hexamer: TATAAA"
     polyA_site      2882
                     /gene="SIRT3"
                     /gene_synonym="SIR2L3"
ORIGIN      
        1 ggactgcgcg gaacatggcg ttctggggtt ggcgcgccgc ggcagccctc cggctgtggg
       61 gccgggtagt tgaacgggtc gaggccgggg gaggcgtggg gccgtttcag gcctgcggct
      121 gtcggctggt gcttggcggc agggacgatg tgagtgcggg gctgagaggc agccatgggg
      181 cccgcggtga gcccttggac ccggcgcgcc ccttgcagag gcctcccaga cccgaggtgc
      241 ccagggcatt ccggaggcag ccgagggcag cagctcccag tttcttcttt tcgagtatta
      301 aaggtggaag aaggtccata tctttttctg tgggtgcttc aagtgttgtt ggaagtggag
      361 gcagcagtga caaggggaag ctttccctgc aggatgtagc tgagctgatt cgggccagag
      421 cctgccagag ggtggtggtc atggtggggg ccggcatcag cacacccagt ggcattccag
      481 acttcagatc gccggggagt ggcctgtaca gcaacctcca gcagtacgat ctcccgtacc
      541 ccgaggccat ttttgaactc ccattcttct ttcacaaccc caagcccttt ttcactttgg
      601 ccaaggagct gtaccctgga aactacaagc ccaacgtcac tcactacttt ctccggctgc
      661 ttcatgacaa ggggctgctt ctgcggctct acacgcagaa catcgatggg cttgagagag
      721 tgtcgggcat ccctgcctca aagctggttg aagctcatgg aacctttgcc tctgccacct
      781 gcacagtctg ccaaagaccc ttcccagggg aggacattcg ggctgacgtg atggcagaca
      841 gggttccccg ctgcccggtc tgcaccggcg ttgtgaagcc cgacattgtg ttctttgggg
      901 agccgctgcc ccagaggttc ttgctgcatg tggttgattt ccccatggca gatctgctgc
      961 tcatccttgg gacctccctg gaggtggagc cttttgccag cttgaccgag gccgtgcgga
     1021 gctcagttcc ccgactgctc atcaaccggg acttggtggg gcccttggct tggcatcctc
     1081 gcagcaggga cgtggcccag ctgggggacg tggttcacgg cgtggaaagc ctagtggagc
     1141 ttctgggctg gacagaagag atgcgggacc ttgtgcagcg ggaaactggg aagcttgatg
     1201 gaccagacaa ataggatgat ggctgccccc acacaataaa tggtaacata ggagacatcc
     1261 acatcccaat tctgacaaga cctcatgcct gaagacagct tgggcaggtg aaaccagaat
     1321 atgtgaactg agtggacacc cgaggctgcc actggaatgt cttctcaggc catgagctgc
     1381 agtgactggt agggctgtgt ttacagtcag ggccaccccg tcacatatac aaaggagctg
     1441 cctgcctgtt tgctgtgttg aactcttcac tctgctgaag ctcctaatgg aaaaagcttt
     1501 cttctgactg tgaccctctt gaactgaatc agaccaactg gaatcccaga ccgagtctgc
     1561 tttctgtgcc tagttgaacg gcaagctcgg catctgttgg ttacaagatc cagacttggg
     1621 ccgagcggtc cccagccctc ttcatgttcc gaagtgtagt cttgaggccc tggtgccgca
     1681 cttctagcat gttggtctcc tttagtgggg ctatttttaa tgagagaaaa tctgttcttt
     1741 ccagcatgaa atacatttag tctcctcaaa gggactgcag gtgttgacat gagttggaaa
     1801 gggaaccctg ggatacgtgg cgtcccctct attggaacag tctgaggact gaaggcattt
     1861 gtccctggat ttattggaga cggcccagct cctccctctg aaggtggtca cattctgttg
     1921 actctccata ctcagcctct cctccagaaa cagatctgtt ccagaacatt ccagcacttt
     1981 ctatctggcc tccttgtccc cacactacgc ccccccaccc tcgccagggc ttcctctagt
     2041 gacactgtta gagctaatct ctgagacagg gaaggcatta ctcacttaaa acccaggctg
     2101 agtcctggcc acctgctgga ttgtgacata ggaggtggaa tccactgaac tgctacttct
     2161 gcacaggctc cttctcctgg ggctgtaccc aggcccagcc ctgatggctc accctgtcag
     2221 gcaccagctg ctccctcctg ggctctcacc cacctgcaca tcctccttcc tagcatcaca
     2281 ttacctgcgt gtttccccag acaaaagcac ttcccattct tgaaccttgc ctaccctggg
     2341 ctgagctgac ggcaatagat ttaatgacag tgactcccag gaagggggtc ctgtgacttt
     2401 gcgccttaat aagaacaaaa ggtggaattg gtgacctagg aaaactgttg aattctaaaa
     2461 agaatgaagt tagtttctaa ccctagttaa tgttcctttt ttattttttg agtcttgccc
     2521 tgtcactcag ggtggagtgc ggtgttatga tctcagctca ctgcaacttc cgcctcccgg
     2581 gtttaagcga ttctcctggg tagctgggat tacaggtgtg tcccaccaca cctagcacat
     2641 gggcatattt gtaatagaga caaggttttg ctatgttggc caggctggtc tcgaactcct
     2701 ggcttcaagt gatccaccca cctcggcctc ccaaagtgct gggattacag gcatgagcca
     2761 ctgtgcctgg cccctttatt tgataattta cacatacatt tttgtccaaa actcttcttt
     2821 atttcaagat gatgtttctg tggctatgtg tggtatgtgg tataaatctc aatctatggt
     2881 ca
//
