LOCUS       NM_015073               8004 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens signal induced proliferation associated 1 like 3
            (SIPA1L3), mRNA.
ACCESSION   NM_015073 XM_032278
VERSION     NM_015073.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8004)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   2  (bases 1 to 8004)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 8004)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 8004)
  AUTHORS   Zhang,R., Lai,L., Dong,X., He,J., You,D., Chen,C., Lin,L., Zhu,Y.,
            Huang,H., Shen,S., Wei,L., Chen,X., Guo,Y., Liu,L., Su,L.,
            Shafer,A., Moran,S., Fleischer,T., Bjaanaes,M.M., Karlsson,A.,
            Planck,M., Staaf,J., Helland,A., Esteller,M., Wei,Y., Chen,F. and
            Christiani,D.C.
  TITLE     SIPA1L3 methylation modifies the benefit of smoking cessation on
            lung adenocarcinoma survival: an epigenomic-smoking interaction
            analysis
  JOURNAL   Mol Oncol 13 (5), 1235-1248 (2019)
   PUBMED   30924596
  REMARK    GeneRIF: smoking cessation benefited survival of LUAD patients with
            low methylation at cg02268510SIPA 1L3 . The results have
            implications for not only smoking cessation after diagnosis, but
            also possible methylation-specific drug targeting.
REFERENCE   5  (bases 1 to 8004)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   6  (bases 1 to 8004)
  AUTHORS   Evers,C., Paramasivam,N., Hinderhofer,K., Fischer,C., Granzow,M.,
            Schmidt-Bacher,A., Eils,R., Steinbeisser,H., Schlesner,M. and
            Moog,U.
  TITLE     SIPA1L3 identified by linkage analysis and whole-exome sequencing
            as a novel gene for autosomal recessive congenital cataract
  JOURNAL   Eur J Hum Genet 23 (12), 1627-1633 (2015)
   PUBMED   25804400
  REMARK    GeneRIF: Whole-exome sequencing identified a homozygous nonsense
            variant in SIPA1L3 in two sisters with congenital cataract.
REFERENCE   7  (bases 1 to 8004)
  AUTHORS   Greenlees,R., Mihelec,M., Yousoof,S., Speidel,D., Wu,S.K.,
            Rinkwitz,S., Prokudin,I., Perveen,R., Cheng,A., Ma,A., Nash,B.,
            Gillespie,R., Loebel,D.A., Clayton-Smith,J., Lloyd,I.C.,
            Grigg,J.R., Tam,P.P., Yap,A.S., Becker,T.S., Black,G.C., Semina,E.
            and Jamieson,R.V.
  TITLE     Mutations in SIPA1L3 cause eye defects through disruption of cell
            polarity and cytoskeleton organization
  JOURNAL   Hum Mol Genet 24 (20), 5789-5804 (2015)
   PUBMED   26231217
  REMARK    GeneRIF: Abnormalities of SIPA1L3 contribute to lens and eye
            defects, and we identify a critical role for SIPA1L3 in epithelial
            cell morphogenesis, polarity, adhesion and cytoskeletal
            organization.
REFERENCE   8  (bases 1 to 8004)
  AUTHORS   Spilker,C. and Kreutz,M.R.
  TITLE     RapGAPs in brain: multipurpose players in neuronal Rap signalling
  JOURNAL   Eur J Neurosci 32 (1), 1-9 (2010)
   PUBMED   20576033
  REMARK    Review article
REFERENCE   9  (bases 1 to 8004)
  AUTHORS   Benzinger,A., Muster,N., Koch,H.B., Yates,J.R. 3rd and Hermeking,H.
  TITLE     Targeted proteomic analysis of 14-3-3 sigma, a p53 effector
            commonly silenced in cancer
  JOURNAL   Mol Cell Proteomics 4 (6), 785-795 (2005)
   PUBMED   15778465
REFERENCE   10 (bases 1 to 8004)
  AUTHORS   Meek,S.E., Lane,W.S. and Piwnica-Worms,H.
  TITLE     Comprehensive proteomic analysis of interphase and mitotic
            14-3-3-binding proteins
  JOURNAL   J Biol Chem 279 (31), 32046-32054 (2004)
   PUBMED   15161933
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC016582.9, BM193478.1,
            BC150620.1, AC011465.4, AC011479.7 and AI560928.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_015073.2.
            
            Summary: This gene belongs to the signal induced proliferation
            associated 1 family of genes, which encode GTPase-activating
            proteins specific for the GTP-binding protein Rap1. Rap1 has been
            implicated in regulation of cell adhesion, cell polarity, and
            organization of the cytoskeleton. Like other members of the family,
            the protein encoded by this gene contains RapGAP and PDZ domains.
            In addition, this protein contains a C-terminal leucine zipper
            domain. This gene is proposed to function in epithelial cell
            morphogenesis and establishment or maintenance of polarity.
            Consistently, expression of the protein in cell culture showed
            localization to cell-cell borders in apical regions, and
            downregulation of the gene in 3D Caco2 cell culture resulted in
            abnormal cell polarity and morphogenesis. Allelic variants of this
            gene have been associated with congenital cataracts in humans.
            [provided by RefSeq, Feb 2016].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC150620.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000222345.11/ ENSP00000222345.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-13                AC016582.9         168436-168448
            14-34               BM193478.1         2-22
            35-1472             BC150620.1         1-1438
            1473-1473           AC011465.4         49117-49117
            1474-2844           BC150620.1         1440-2810
            2845-3379           AC011465.4         85938-86472
            3380-4464           BC150620.1         3346-4430
            4465-4465           AC011479.7         154744-154744       c
            4466-6360           BC150620.1         4432-6326
            6361-7920           AC011479.7         111093-112652       c
            7921-8004           AI560928.1         28-111              c
FEATURES             Location/Qualifiers
     source          1..8004
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.13-q13.2"
     gene            1..8004
                     /gene="SIPA1L3"
                     /gene_synonym="CTRCT45; SPAL3; SPAR3"
                     /note="signal induced proliferation associated 1 like 3"
                     /db_xref="GeneID:23094"
                     /db_xref="HGNC:HGNC:23801"
                     /db_xref="MIM:616655"
     exon            1..151
                     /gene="SIPA1L3"
                     /gene_synonym="CTRCT45; SPAL3; SPAR3"
                     /inference="alignment:Splign:2.1.0"
     exon            152..219
                     /gene="SIPA1L3"
                     /gene_synonym="CTRCT45; SPAL3; SPAR3"
                     /inference="alignment:Splign:2.1.0"
     exon            220..2063
                     /gene="SIPA1L3"
                     /gene_synonym="CTRCT45; SPAL3; SPAR3"
                     /inference="alignment:Splign:2.1.0"
     CDS             530..5875
                     /gene="SIPA1L3"
                     /gene_synonym="CTRCT45; SPAL3; SPAR3"
                     /note="SIPA1-like protein 3; SPA-1-like protein 3;
                     spine-associated RapGAP 3"
                     /codon_start=1
                     /product="signal-induced proliferation-associated 1-like
                     protein 3"
                     /protein_id="NP_055888.1"
                     /db_xref="CCDS:CCDS33007.1"
                     /db_xref="GeneID:23094"
                     /db_xref="HGNC:HGNC:23801"
                     /db_xref="MIM:616655"
                     /translation="MTTYRAIPSDGVDLAASCGARVGDVLPGPHTGDYAPLGFWAQNG
                     SMSQPLGESPATATATATATTRPSPTTPAMPKMGVRARVADWPPKREALREHSNPSPS
                     QDTDGTKATKMAHSMRSIQNGQPPTSTPASSGSKAFHRLSRRRSKDVEFQDGWPRSPG
                     RAFLPLRHRSSSEITLSECDAEDAGEPRGARHTGALPLFREYGSTSSIDVQGMPEQSF
                     FDILNEFRSEQPDARGCQALTELLRADPGPHLMGGGGGAKGDSHNGQPAKDSLLPLQP
                     TKEKEKARKKPARGLGGGDTVDSSIFRKLRSSKPEGEAGRSPGEADEGRSPPEASRPW
                     VCQKSFAHFDVQSMLFDLNEAAANRVSVSQRRNTTTGASAASAASAMASLTASRAHSL
                     GGLDPAFTSTEDLNCKENLEQDLGDDNSNDLLLSCPHFRNEIGGECERNVSFSRASVG
                     SPSSGEGHLAEPALSAYRTNASISVLEVPKEQQRTQSRPRQYSIEHVDLGARYYQDYF
                     VGKEHANYFGVDEKLGPVAVSIKREKLEDHKEHGPQYQYRIIFRTRELITLRGSILED
                     ATPTATKHGTGRGLPLKDALEYVIPELNIHCLRLALNTPKVTEQLLKLDEQGLCRKHK
                     VGILYCKAGQSSEEEMYNNEEAGPAFEEFLSLIGEKVCLKGFTKYAAQLDVKTDSTGT
                     HSLYTMYQDYEIMFHVSTLLPYTPNNRQQLLRKRHIGNDIVTIIFQEPGALPFTPKNI
                     RSHFQHVFIIVRVHNPCTDNVCYSMAVTRSKDAPPFGPPIPSGTTFRKSDVFRDFLLA
                     KVINAENAAHKSDKFHTMATRTRQEYLKDLAENCVSNTPIDSTGKFNLISLTSKKKEK
                     TKARAGAEQHSAGAIAWRVVAQDYAQGVEIDCILGISNEFVVLLDLRTKEVVFNCYCG
                     DVIGWTPDSSTLKIFYGRGDHIFLQATEGSVEDIREIVQRLKVMTSGWETVDMTLRRN
                     GLGQLGFHVKYDGTVAEVEDYGFAWQAGLRQGSRLVEICKVAVVTLTHDQMIDLLRTS
                     VTVKVVIIPPFEDGTPRRGWPETYDMNTSEPKTEQESITPGGRPPYRSNAPWQWSGPA
                     SHNSLPASKWATPTTPGHAQSLSRPLKQTPIVPFRESQPLHSKRPVSFPETPYTVSPA
                     GADRVPPYRQPSGSFSTPGSATYVRYKPSPERYTAAPHPLLSLDPHFSHDGTSSGDSS
                     SGGLTSQESTMERQKPEPLWHVPAQARLSAIAGSSGNKHPSRQDAAGKDSPNRHSKGE
                     PQYSSHSSSNTLSSNASSSHSDDRWFDPLDPLEPEQDPLSKGGSSDSGIDTTLYTSSP
                     SCMSLAKAPRPAKPHKPPGSMGLCGGGREAAGRSHHADRRREVSPAPAVAGQSKGYRP
                     KLYSSGSSTPTGLAGGSRDPPRQPSDMGSRVGYPAQVYKTASAETPRPSQLAQPSPFQ
                     LSASVPKSFFSKQPVRNKHPTGWKRTEEPPPRPLPFSDPKKQVDTNTKNVFGQPRLRA
                     SLRDLRSPRKNYKSTIEDDLKKLIIMDNLGPEQERDTGQSPQKGLQRTLSDESLCSGR
                     REPSFASPAGLEPGLPSDVLFTSTCAFPSSTLPARRQHQHPHPPVGPGATPAAGSGFP
                     EKKSTISASELSLADGRDRPLRRLDPGLMPLPDTAAGLEWSSLVNAAKAYEVQRAVSL
                     FSLNDPALSPDIPPAHSPVHSHLSLERGPPTPRTTPTMSEEPPLDLTGKVYQLEVMLK
                     QLHTDLQKEKQDKVVLQSEVASLRQNNQRLQEESQAASEQLRKFAEIFCREKKEL"
     exon            2064..2194
                     /gene="SIPA1L3"
                     /gene_synonym="CTRCT45; SPAL3; SPAR3"
                     /inference="alignment:Splign:2.1.0"
     exon            2195..2383
                     /gene="SIPA1L3"
                     /gene_synonym="CTRCT45; SPAL3; SPAR3"
                     /inference="alignment:Splign:2.1.0"
     exon            2384..2558
                     /gene="SIPA1L3"
                     /gene_synonym="CTRCT45; SPAL3; SPAR3"
                     /inference="alignment:Splign:2.1.0"
     exon            2559..2662
                     /gene="SIPA1L3"
                     /gene_synonym="CTRCT45; SPAL3; SPAR3"
                     /inference="alignment:Splign:2.1.0"
     exon            2663..2820
                     /gene="SIPA1L3"
                     /gene_synonym="CTRCT45; SPAL3; SPAR3"
                     /inference="alignment:Splign:2.1.0"
     exon            2821..3397
                     /gene="SIPA1L3"
                     /gene_synonym="CTRCT45; SPAL3; SPAR3"
                     /inference="alignment:Splign:2.1.0"
     exon            3398..3672
                     /gene="SIPA1L3"
                     /gene_synonym="CTRCT45; SPAL3; SPAR3"
                     /inference="alignment:Splign:2.1.0"
     exon            3673..3924
                     /gene="SIPA1L3"
                     /gene_synonym="CTRCT45; SPAL3; SPAR3"
                     /inference="alignment:Splign:2.1.0"
     exon            3925..4062
                     /gene="SIPA1L3"
                     /gene_synonym="CTRCT45; SPAL3; SPAR3"
                     /inference="alignment:Splign:2.1.0"
     exon            4063..4190
                     /gene="SIPA1L3"
                     /gene_synonym="CTRCT45; SPAL3; SPAR3"
                     /inference="alignment:Splign:2.1.0"
     exon            4191..4309
                     /gene="SIPA1L3"
                     /gene_synonym="CTRCT45; SPAL3; SPAR3"
                     /inference="alignment:Splign:2.1.0"
     exon            4310..4737
                     /gene="SIPA1L3"
                     /gene_synonym="CTRCT45; SPAL3; SPAR3"
                     /inference="alignment:Splign:2.1.0"
     exon            4738..4959
                     /gene="SIPA1L3"
                     /gene_synonym="CTRCT45; SPAL3; SPAR3"
                     /inference="alignment:Splign:2.1.0"
     exon            4960..5125
                     /gene="SIPA1L3"
                     /gene_synonym="CTRCT45; SPAL3; SPAR3"
                     /inference="alignment:Splign:2.1.0"
     exon            5126..5369
                     /gene="SIPA1L3"
                     /gene_synonym="CTRCT45; SPAL3; SPAR3"
                     /inference="alignment:Splign:2.1.0"
     exon            5370..5513
                     /gene="SIPA1L3"
                     /gene_synonym="CTRCT45; SPAL3; SPAR3"
                     /inference="alignment:Splign:2.1.0"
     exon            5514..5649
                     /gene="SIPA1L3"
                     /gene_synonym="CTRCT45; SPAL3; SPAR3"
                     /inference="alignment:Splign:2.1.0"
     exon            5650..5731
                     /gene="SIPA1L3"
                     /gene_synonym="CTRCT45; SPAL3; SPAR3"
                     /inference="alignment:Splign:2.1.0"
     exon            5732..8004
                     /gene="SIPA1L3"
                     /gene_synonym="CTRCT45; SPAL3; SPAR3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7986..7991
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SIPA1L3"
                     /gene_synonym="CTRCT45; SPAL3; SPAR3"
                     /note="hexamer: AATAAA"
     polyA_site      8004
                     /gene="SIPA1L3"
                     /gene_synonym="CTRCT45; SPAL3; SPAR3"
                     /note="major polyA site"
ORIGIN      
        1 acagttccca ggaggactcg cgcgaagcgg cttggaagtg ggcagcctgg aagtcactcg
       61 ggccaccgga gctggcggcg tctccagcga gcggcgcgga gctggacgcc gcgatcgtgg
      121 ggcccgcgag gcgggcagcc ggggcgtcca gatgcacagt ctctgacatc agctctcctg
      181 ggaccctcca agagcactag tgtctagaag gcactaaggc atggcgagga caacatcctt
      241 gctgcctcca gctggcgggc gaccacagct actgcctgct ctgcgctact gagccactcg
      301 gtctggagcc cccaggacag cacctgcttc ctgaggttgt cctggctcag ctgtgcacag
      361 cgatggtgga gaactggact ccacaggctc acaaccttcc tgtcaggttt cagggcccag
      421 catccttcat cctgggcctg gctgccctga acaatggctg agggctgggg gaccccatag
      481 agtgacacca cagcgtacgg ggccagcagc actccagtgc ccgtggacta tgaccaccta
      541 tcgggccatc cccagcgatg gtgtggacct ggcagccagc tgtggcgcca gggtgggcga
      601 tgtcctccct gggccacaca caggggacta cgctcccttg ggattctggg cccagaatgg
      661 cagcatgtcc cagcctcttg gcgagagccc ggccaccgcc accgccaccg ccaccgccac
      721 cacccgcccc agccccacca ctcccgcaat gcccaagatg ggcgtgcgcg caagggtggc
      781 cgactggccg cccaagcggg aggccctgag agagcacagc aacccaagcc cctcccagga
      841 cacagatggc acaaaggcca ccaagatggc ccattccatg aggagcatac agaacggaca
      901 gccccccacc agcaccccgg cttcctcagg gtccaaagcc ttccaccgac tctccaggag
      961 aaggtccaaa gacgtggagt tccaggacgg gtggccccgg tcccccggca gggccttcct
     1021 cccccttcgg caccgcagca gcagcgagat caccctcagc gagtgtgacg cggaggacgc
     1081 gggggagccg cggggggccc ggcacacggg ggcgctgccc ctcttccgcg agtacgggag
     1141 cacctcgtcc atcgacgtgc agggcatgcc cgagcagagc ttcttcgaca tcctgaacga
     1201 gttccgcagc gagcagcccg acgcccgagg gtgccaggcc ctcaccgagc tcctccgggc
     1261 agatcctggc ccacacctca tggggggcgg cggcggagcc aagggggact cccacaacgg
     1321 gcagcccgcc aaggacagcc tcctgccact gcagcccacg aaggagaagg agaaggcccg
     1381 gaagaaacct gcgcggggcc tcggcggcgg ggacacggtg gactcgtcca tctttcggaa
     1441 gctaaggagc agcaaacccg agggggaggc tgggcgttcc ccgggggagg ccgacgaggg
     1501 ccggagcccc ccggaagcca gcaggccgtg ggtgtgtcag aagagcttcg cccacttcga
     1561 cgtgcagagc atgctgttcg acctcaacga ggcggccgcc aacagggtgt cggtgtcgca
     1621 gcggcggaac accaccacgg gtgcttcggc cgcttccgcc gcctcggcca tggcctccct
     1681 cacggcctcg cgggcccaca gcctcggagg cctggacccg gccttcacca gcacagagga
     1741 cctaaactgc aaggagaact tggagcagga cctcggcgat gacaacagca acgacctgct
     1801 gctcagctgc ccgcacttcc gcaatgagat cgggggcgag tgtgagcgca acgtgagctt
     1861 ctcccgggct tccgtgggct ccccgagcag cggcgagggc cacctggcag agcccgccct
     1921 gagcgcctac cgcaccaacg ccagcatctc ggtgttggaa gttcccaagg agcagcagcg
     1981 gacgcagagt cggccccggc agtacagcat cgagcatgtg gacctgggcg cccgctacta
     2041 ccaggattac ttcgtgggca aagaacatgc caattacttc ggcgtggatg agaagctggg
     2101 gccagtggct gtgagcatta agcgggagaa gctggaagac cacaaggagc acggacctca
     2161 gtaccagtac aggatcatct tccggacccg cgagctcatc accctgcggg gctccatcct
     2221 ggaagatgct acgcccacag ccaccaagca tgggaccggg cggggcctgc ccttgaagga
     2281 tgccctggag tatgtcatcc ccgagctcaa catccactgc ctgcggctgg ccctcaacac
     2341 ccccaaggtg acggagcaac tgctgaagct cgatgagcaa gggctctgcc ggaagcacaa
     2401 ggtgggcatc ctctattgca aggccggcca gagctccgag gaggagatgt acaacaatga
     2461 ggaggccggc cccgcctttg aggagttcct ctccctcatc ggcgagaagg tctgcctgaa
     2521 gggcttcacc aagtacgctg cccagctgga cgtcaagacc gactccacgg gaacccactc
     2581 cctctacacg atgtaccagg actacgagat catgttccat gtctccaccc tgctccctta
     2641 cacccccaac aacaggcagc agctgctacg gaagaggcac ataggaaatg acatcgtgac
     2701 gatcatcttc caggagcctg gcgcgctacc gttcaccccc aagaacatcc gctcccactt
     2761 ccagcacgtc ttcatcattg tccgagtcca caacccctgc actgataacg tctgttacag
     2821 tatggctgtg acccgatcca aagacgctcc tcctttcggc ccccccatcc ccagtggaac
     2881 cacattccgc aaatccgacg tcttcagaga cttcttgctg gccaaggtga ttaacgctga
     2941 gaacgccgcg cacaagtccg acaagttcca caccatggcc accaggaccc gccaggagta
     3001 tctcaaggac ctggccgaaa actgtgtctc caacaccccc atcgactcca ccggcaaatt
     3061 caacctcatc tccctgacct ccaagaagaa ggaaaagaca aaagcacggg ctggcgctga
     3121 gcagcacagt gcaggggcca tcgcctggag ggtggtggcc caggactacg cccagggggt
     3181 ggaaatcgac tgcattttgg gaatttccaa tgagtttgtg gtgctcctgg acttacgcac
     3241 caaggaggtg gtgttcaact gctactgcgg ggatgtcatt ggctggactc cagactcctc
     3301 cacactcaaa atcttctatg gacgaggaga ccacatcttc ctacaggcga cagagggttc
     3361 tgtggaggac ataagggaga tagtgcagag actgaaggtg atgaccagtg gctgggagac
     3421 ggtggacatg acgcttcggc ggaacgggct cgggcagctg ggcttccacg tgaagtacga
     3481 cggcacggtg gccgaggttg aggactatgg gttcgcctgg caggccggcc tccggcaggg
     3541 cagccgacta gtggagatct gcaaggtggc cgtggtcaca ctgacccacg accagatgat
     3601 cgacctgctg cgcacctctg tcactgtgaa ggtggtcatc atcccgcctt ttgaggacgg
     3661 cactccccgg aggggttggc cggagaccta cgacatgaat acctcggagc ccaagacgga
     3721 gcaggaaagc atcactcctg ggggccggcc cccctaccgc agcaatgctc cctggcagtg
     3781 gagcgggccc gcatcccata actctctacc agcctccaag tgggccactc caaccactcc
     3841 cggccatgcc cagtccctga gccggcccct gaagcagacc cccatagtcc ccttccggga
     3901 gtcccagcca ctgcacagca agaggcctgt cagcttccca gaaacccctt acacagtatc
     3961 accagcaggg gccgacagag tccctcccta ccgacagcct tctgggagct tctccacccc
     4021 cggttcggcc acctacgtga gatacaagcc atccccagaa aggtacacgg ctgccccaca
     4081 ccccctgcta tctcttgatc cccacttcag ccacgatggg acgtccagcg gcgactcctc
     4141 ttccggcggc ctgaccagcc aggagagcac catggaacgc cagaagccag agcctttgtg
     4201 gcatgtgcct gcccaggcca ggctctcagc catagccgga agcagcggga acaagcaccc
     4261 gtccaggcag gatgcagcag gcaaagattc ccccaacagg cattccaaag gagaacctca
     4321 atactcaagt cattccagca gcaacaccct ctccagcaac gcatccagca gccacagcga
     4381 cgaccgctgg ttcgaccccc tggaccccct ggagccagag caagaccccc tctccaaggg
     4441 tggctctagt gacagcggca tcgacaccac cctctacacc tccagcccta gctgcatgtc
     4501 cctggccaag gctccacggc ccgccaagcc acacaagccc cctggaagta tgggcctttg
     4561 tggcgggggt cgcgaggccg ctgggaggtc ccaccacgca gacaggcggc gggaggtctc
     4621 ccctgccccc gcagttgccg gccaaagcaa gggctaccga ccgaagctgt actcctccgg
     4681 ctccagcacc cccacgggac tggcgggggg cagccgagac ccaccgaggc agcccagtga
     4741 catgggctcg agggttggct accccgctca ggtttacaaa actgccagtg cagagactcc
     4801 tcggccctcc cagctggccc agcccagccc ctttcagctc tccgcctccg tccccaagtc
     4861 cttcttctcc aagcagcctg tacgcaataa gcacccaaca gggtggaaga gaacggagga
     4921 gcccccacca cggccactcc ccttcagtga cccaaagaag caggtggaca cgaacaccaa
     4981 aaatgtcttt gggcaaccga ggttgagggc atccctccga gacctccggt caccacggaa
     5041 gaactacaaa tccaccatcg aggatgacct gaagaaactc atcatcatgg acaacctggg
     5101 gccagagcag gagagagaca cgggacagtc accgcagaag ggcctgcagc ggacgctgtc
     5161 ggacgagagc ctgtgcagcg ggcgccggga gcccagcttc gccagccccg ctggcctaga
     5221 gccagggctg cccagcgacg tgctcttcac cagcacctgc gccttcccgt ccagcacgct
     5281 gcctgcacgc cgccagcacc agcaccccca cccgcccgtc ggccccggtg ccacccctgc
     5341 cgccggcagc ggctttcccg agaagaaatc caccatctca gcctcggagc tctcgctggc
     5401 tgatgggcgg gaccgccccc tgcggcgcct ggaccctggg ctgatgcccc tgcctgacac
     5461 agctgctggc ctcgagtggt ccagcctggt gaacgcagcc aaggcatacg aagtgcaaag
     5521 agccgtctca ctcttctctc tgaacgaccc ggccctgagc ccggacatcc cgcctgcaca
     5581 cagtcctgtc cacagccacc tgagcctgga gaggggaccc ccgaccccca ggaccacccc
     5641 taccatgagc gaggagccac ccctggatct gacaggcaag gtgtaccagc tggaggtgat
     5701 gctgaaacag ctgcacactg acctgcagaa ggagaagcag gacaaggtgg tgctccagtc
     5761 agaggtggcc agcctgcggc agaacaacca gcggctgcag gaggagtcgc aggccgccag
     5821 cgagcagctg cgcaagtttg cggagatctt ctgcagggag aagaaggagc tctgaggtgg
     5881 gaggccgccg cccgccttcg ctccttcccc tcaggccgtg gccctgctgc ctctctccct
     5941 ccactcagct cccagctgcc ggtgtgacca agatgaccca tccagggccc tccccatgga
     6001 cacggaaact ccgatggcct cactagggct gtgagtcagg gtcagcgcgc acagccctca
     6061 tgccccagag ggcgaagtgg tctcaggcct ccctccaagc tgcccagctg tggtcccggt
     6121 gagctgggct gtgcttacac cgctcccggg cctgccccgc tgtccccatc tagcctcttc
     6181 ctgggaccag cccttcgaag ctgatgggga cggagagagg agccagtctc cagacgcctc
     6241 ggctccagga ggtcacacag caggtttctg caccacgcac aacaaagttg cggcttgtgg
     6301 ggccgaggcc cctgcatgaa aagtacagcg tgaaagcaac cctttcttcc ccacacccag
     6361 agaggtgagg cccgcgtgga cacagaccag ggcgcctcgg tccctagcac gtgagctcca
     6421 aaaagcacga acatgatccc ctccctaacc ccctcctccc aaaaagcagc caggagcact
     6481 ttcttagagt ttaaattatt ttcttggggg cctgaggata gagagaggca aggctagcct
     6541 ccagagccga tttatttgag agagaaactc tatttggatc tccaggacgg tttatacgat
     6601 ctttttctat cagctcctct tccgccagag gccactgcac ctgggctagg ggtccacagg
     6661 cccctccacc ttccagctaa ccaggccagg ccggggcggt ggagccacca cacagccgac
     6721 tctctcctcc tcctcccggc acgacctggc cctgaccagc agcctcggcc tcttctgggt
     6781 tccaggaggg gctcccgaag ccctcaggac agggctggag gagaacagcc ttcatctcct
     6841 cctctggttt ttttttcctt tgcaagtctc ccagcccatc tctccgagca gcggccacca
     6901 gaccccctca ccatgctccc cggggagcac tgcaccccgc gaaccccact ggacgctggt
     6961 tctctaaagg caataagggg cggccatgcc ggtgtggtac cacgctgttc ctccaggaaa
     7021 ccgtttcttt ttttccttct ttaaaagcaa acagaaaaag atatatatat atatatatat
     7081 atatttattt tttcatgtag agttggcgcc agaacaagcc tgtatggcca cagtctgtgg
     7141 attcatcccc ccaacctcaa gctgaggacc aaggcgtgtc ccctgtgggc tgggggtcag
     7201 tgggaggacc agagggatga agtcagggca ggccggtgcc ctttttggga ggcaccaggc
     7261 ggggaggagt tggcggagca ggtctggctg tgagccagca ccaggcaacc cggcccttgt
     7321 ccagggacct ctgctgcctt ctctctgggg tcaggaacct cagaggaggg ggctctgggg
     7381 actgcatagg acgcaggcac tcggaggtgc cggtgtggat gggtgggggt gagagaagag
     7441 ggttcttatc gcgtctcctg tgatcagcct ctggccccct ggaggaggaa acctcagcgc
     7501 tgcccctgcc cccaccctca cccccaacac caagagtgag gtgggcgtgc cccaggcagc
     7561 gtcctcagcc ctggccagct actgtgatgc catcttctcc cccatcccct tctctggggg
     7621 gcccacccgt cccccacccc caccccttag accctcatcg ggtccctttt tttttttttt
     7681 tttcagttgt ctcctcccat cttcagatca ttcgaatcat tttggggact taatcatgta
     7741 catggacaag tgtaaattat gatttttggt tttgttttct gttatttttt aaggatttct
     7801 gcaaaaacag atctatttaa tttgaggttg atgttctatc caatggccga agatagcagc
     7861 aggttttttt tttcatatgt tgatttgttt catttggatt ttttttttat tattctttat
     7921 tgtctttttt ttttccccat ccctgtcttg agattttctg gcatgtttct ggaggtttca
     7981 aaggaaataa aggtttcata gaaa
//
