LOCUS       NM_005068               8428 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens SIM bHLH transcription factor 1 (SIM1), transcript
            variant 1, mRNA.
ACCESSION   NM_005068 XM_005267100
VERSION     NM_005068.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8428)
  AUTHORS   Sweed,D., Elhamed,S.M.A., Aiad,H.A.S., Ehsan,N.A., Hemida,A.S. and
            Dawoud,M.M.
  TITLE     STIM1/SOX2 proteins are co-expressed in the tumor and
            microenvironmental stromal cells of pancreatic ductal
            adenocarcinoma and ampullary carcinoma
  JOURNAL   World J Surg Oncol 22 (1), 84 (2024)
   PUBMED   38532463
  REMARK    GeneRIF: STIM1/SOX2 proteins are co-expressed in the tumor and
            microenvironmental stromal cells of pancreatic ductal
            adenocarcinoma and ampullary carcinoma.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8428)
  AUTHORS   Coban,M.A., Blackburn,P.R., Whitelaw,M.L., Haelst,M.M.V.,
            Atwal,P.S. and Caulfield,T.R.
  TITLE     Structural Models for the Dynamic Effects of Loss-of-Function
            Variants in the Human SIM1 Protein Transcriptional Activation
            Domain
  JOURNAL   Biomolecules 10 (9), 1314 (2020)
   PUBMED   32932609
  REMARK    GeneRIF: Structural Models for the Dynamic Effects of
            Loss-of-Function Variants in the Human SIM1 Protein Transcriptional
            Activation Domain.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 8428)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 8428)
  AUTHORS   Jorgenson,E., Matharu,N., Palmer,M.R., Yin,J., Shan,J.,
            Hoffmann,T.J., Thai,K.K., Zhou,X., Hotaling,J.M., Jarvik,G.P.,
            Ahituv,N., Wessells,H. and Van Den Eeden,S.K.
  TITLE     Genetic variation in the SIM1 locus is associated with erectile
            dysfunction
  JOURNAL   Proc Natl Acad Sci U S A 115 (43), 11018-11023 (2018)
   PUBMED   30297428
  REMARK    GeneRIF: SIM1 is part of the leptin-melanocortin system.
REFERENCE   5  (bases 1 to 8428)
  AUTHORS   Kim,H.J., Kim,C.Y., Jin,J., Bae,M.K., Kim,Y.H., Ju,W., Kim,Y.H. and
            Kim,S.C.
  TITLE     Aberrant single-minded homolog 1 methylation as a potential
            biomarker for cervical cancer
  JOURNAL   Diagn Cytopathol 46 (1), 15-21 (2018)
   PUBMED   29063719
  REMARK    GeneRIF: SIM1 was highly methylated in the majority of the cervical
            cancer tissues.  Hypermethylation of SIM1 led to a pronounced
            reduction in SIM1 expression in cervical cancer tissues compared
            with normal cervix.  The degree of SIM1 methylation was
            significantly associated with the severity of the disease.
REFERENCE   6  (bases 1 to 8428)
  AUTHORS   Michaud,J.L., Boucher,F., Melnyk,A., Gauthier,F., Goshu,E.,
            Levy,E., Mitchell,G.A., Himms-Hagen,J. and Fan,C.M.
  TITLE     Sim1 haploinsufficiency causes hyperphagia, obesity and reduction
            of the paraventricular nucleus of the hypothalamus
  JOURNAL   Hum Mol Genet 10 (14), 1465-1473 (2001)
   PUBMED   11448938
REFERENCE   7  (bases 1 to 8428)
  AUTHORS   Holder,J.L. Jr., Butte,N.F. and Zinn,A.R.
  TITLE     Profound obesity associated with a balanced translocation that
            disrupts the SIM1 gene
  JOURNAL   Hum Mol Genet 9 (1), 101-108 (2000)
   PUBMED   10587584
REFERENCE   8  (bases 1 to 8428)
  AUTHORS   Chrast,R., Scott,H.S., Chen,H., Kudoh,J., Rossier,C., Minoshima,S.,
            Wang,Y., Shimizu,N. and Antonarakis,S.E.
  TITLE     Cloning of two human homologs of the Drosophila single-minded gene
            SIM1 on chromosome 6q and SIM2 on 21q within the Down syndrome
            chromosomal region
  JOURNAL   Genome Res 7 (6), 615-624 (1997)
   PUBMED   9199934
REFERENCE   9  (bases 1 to 8428)
  AUTHORS   Probst,M.R., Fan,C.M., Tessier-Lavigne,M. and Hankinson,O.
  TITLE     Two murine homologs of the Drosophila single-minded protein that
            interact with the mouse aryl hydrocarbon receptor nuclear
            translocator protein
  JOURNAL   J Biol Chem 272 (7), 4451-4457 (1997)
   PUBMED   9020169
REFERENCE   10 (bases 1 to 8428)
  AUTHORS   Fan,C.M., Kuwana,E., Bulfone,A., Fletcher,C.F., Copeland,N.G.,
            Jenkins,N.A., Crews,S., Martinez,S., Puelles,L., Rubenstein,J.L.
            and Tessier-Lavigne,M.
  TITLE     Expression patterns of two murine homologs of Drosophila
            single-minded suggest possible roles in embryonic patterning and in
            the pathogenesis of Down syndrome
  JOURNAL   Mol Cell Neurosci 7 (1), 1-16 (1996)
   PUBMED   8812055
  REMARK    Erratum:[Mol Cell Neurosci 1996 Jun;7(6):519]
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from Z86062.1 and AL121948.8.
            This sequence is a reference standard in the RefSeqGene project.
            
            On or before Oct 11, 2019 this sequence version replaced
            XM_005267100.2, NM_005068.2.
            
            Summary: SIM1 and SIM2 genes are Drosophila single-minded (sim)
            gene homologs.  SIM1 transcript was detected only in fetal kidney
            out of various adult and fetal tissues tested.  Since the sim gene
            plays an important role in Drosophila development and has peak
            levels of expression during the period of neurogenesis,it was
            proposed that the human SIM gene is a candidate for involvement in
            certain dysmorphic features (particularly the facial and skull
            characteristics), abnormalities of brain development, and/or
            cognitive disability of Down syndrome. [provided by RefSeq, Jul
            2008].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK308090.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1970526, SAMEA2145774
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000369208.8/ ENSP00000358210.4
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-308               Z86062.1           64205-64512         c
            309-950             Z86062.1           62885-63526         c
            951-1033            Z86062.1           53353-53435         c
            1034-1123           Z86062.1           49858-49947         c
            1124-1232           Z86062.1           49182-49290         c
            1233-1318           Z86062.1           48954-49039         c
            1319-1518           Z86062.1           48070-48269         c
            1519-1625           Z86062.1           47737-47843         c
            1626-1773           Z86062.1           46859-47006         c
            1774-1942           Z86062.1           20381-20549         c
            1943-2345           AL121948.8         13005-13407         c
            2346-8428           AL121948.8         4527-10609          c
FEATURES             Location/Qualifiers
     source          1..8428
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q16.3"
     gene            1..8428
                     /gene="SIM1"
                     /gene_synonym="bHLHe14"
                     /note="SIM bHLH transcription factor 1"
                     /db_xref="GeneID:6492"
                     /db_xref="HGNC:HGNC:10882"
                     /db_xref="MIM:603128"
     exon            1..308
                     /gene="SIM1"
                     /gene_synonym="bHLHe14"
                     /inference="alignment:Splign:2.1.0"
     exon            309..950
                     /gene="SIM1"
                     /gene_synonym="bHLHe14"
                     /inference="alignment:Splign:2.1.0"
     CDS             776..3076
                     /gene="SIM1"
                     /gene_synonym="bHLHe14"
                     /note="class E basic helix-loop-helix protein 14;
                     single-minded family bHLH transcription factor 1"
                     /codon_start=1
                     /product="single-minded homolog 1"
                     /protein_id="NP_005059.2"
                     /db_xref="CCDS:CCDS5045.1"
                     /db_xref="GeneID:6492"
                     /db_xref="HGNC:HGNC:10882"
                     /db_xref="MIM:603128"
                     /translation="MKEKSKNAARTRREKENSEFYELAKLLPLPSAITSQLDKASIIR
                     LTTSYLKMRVVFPEGLGEAWGHSSRTSPLDNVGRELGSHLLQTLDGFIFVVAPDGKIM
                     YISETASVHLGLSQVELTGNSIYEYIHPADHDEMTAVLTAHQPYHSHFVQEYEIERSF
                     FLRMKCVLAKRNAGLTCGGYKVIHCSGYLKIRQYSLDMSPFDGCYQNVGLVAVGHSLP
                     PSAVTEIKLHSNMFMFRASLDMKLIFLDSRVAELTGYEPQDLIEKTLYHHVHGCDTFH
                     LRCAHHLLLVKGQVTTKYYRFLAKHGGWVWVQSYATIVHNSRSSRPHCIVSVNYVLTD
                     TEYKGLQLSLDQISASKPAFSYTSSSTPTMTDNRKGAKSRLSSSKSKSRTSPYPQYSG
                     FHTERSESDHDSQWGGSPLTDTASPQLLDPADRPGSQHDASCAYRQFSDRSSLCYGFA
                     LDHSRLVEERHFHTQACEGGRCEAGRYFLGTPQAGREPWWGSRAALPLTKASPESREA
                     YENSMPHIASVHRIHGRGHWDEDSVVSSPDPGSASESGDRYRTEQYQSSPHEPSKIET
                     LIRATQQMIKEEENRLQLRKAPSDQLASINGAGKKHSLCFANYQQPPPTGEVCHGSAL
                     ANTSPCDHIQQREGKMLSPHENDYDNSPTALSRISSPNSDRISKSSLILAKDYLHSDI
                     SPHQTAGDHPTVSPNCFGSHRQYFDKHAYTLTGYALEHLYDSETIRNYSLGCNGSHFD
                     VTSHLRMQPDPAQGHKGTSVIITNGS"
     exon            951..1033
                     /gene="SIM1"
                     /gene_synonym="bHLHe14"
                     /inference="alignment:Splign:2.1.0"
     exon            1034..1123
                     /gene="SIM1"
                     /gene_synonym="bHLHe14"
                     /inference="alignment:Splign:2.1.0"
     exon            1124..1232
                     /gene="SIM1"
                     /gene_synonym="bHLHe14"
                     /inference="alignment:Splign:2.1.0"
     exon            1233..1318
                     /gene="SIM1"
                     /gene_synonym="bHLHe14"
                     /inference="alignment:Splign:2.1.0"
     exon            1319..1518
                     /gene="SIM1"
                     /gene_synonym="bHLHe14"
                     /inference="alignment:Splign:2.1.0"
     exon            1519..1625
                     /gene="SIM1"
                     /gene_synonym="bHLHe14"
                     /inference="alignment:Splign:2.1.0"
     exon            1626..1773
                     /gene="SIM1"
                     /gene_synonym="bHLHe14"
                     /inference="alignment:Splign:2.1.0"
     exon            1774..1942
                     /gene="SIM1"
                     /gene_synonym="bHLHe14"
                     /inference="alignment:Splign:2.1.0"
     exon            1943..2345
                     /gene="SIM1"
                     /gene_synonym="bHLHe14"
                     /inference="alignment:Splign:2.1.0"
     exon            2346..8428
                     /gene="SIM1"
                     /gene_synonym="bHLHe14"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7323..7328
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SIM1"
                     /gene_synonym="bHLHe14"
                     /note="hexamer: AATAAA"
     polyA_site      7342
                     /gene="SIM1"
                     /gene_synonym="bHLHe14"
     regulatory      8403..8408
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SIM1"
                     /gene_synonym="bHLHe14"
                     /note="hexamer: AATATA"
     polyA_site      8428
                     /gene="SIM1"
                     /gene_synonym="bHLHe14"
                     /note="major polyA site"
ORIGIN      
        1 aagctataaa gaagttacgg ggggcagttt tcggcttcca attaggaata atagtgaact
       61 ggcttcgtag caactacgga ggaccaggat tctaaaatca cctcactcgt cccaaagctt
      121 gcatcctcct ctttctgcca cgcacccctc ctccaattca tgatccagaa aagggagccg
      181 ggaatgctct gctcctctct gccggtggga agcggagagg ccggcggtgt cgctgggttg
      241 gacggtaggc atgagaacag ttaagagatg ggcgcccccg aaacctctgc cgcttgtggg
      301 gactgaaggg ccttgaaaga gttgtctggg gttcctgctc ttcccaaagg ggctcggaga
      361 taccttaaat gccgcgagac tgaagagcaa acgtcaggga ttattatgtc gattttccga
      421 ggaccagggt tcgcagctgg cagtagaaga aaaggctggc tcctcagggg tcccactggc
      481 atttgatacc ctgaacgacc ctcaggattg cccaaattcg gtggatcgcc caaattcggt
      541 ggatcagcaa ctttcctata ctgcatcccg aattttcaaa gaacatattt tccgttcacc
      601 cccgctggtc ttttactgcc atcaatacac tgttcttggt gcaaatactt cagcctcttt
      661 attcaaagta tgttttatgt ttttgccaaa tatgatctct aattgaaagt ttatttttgg
      721 ttttggatga atctgcggag cttaagttgt gagaagaaag ggggaacaag acacaatgaa
      781 agaaaagtcc aaaaatgctg cgcggactag gagggagaag gaaaacagtg aattttatga
      841 actggctaaa ttactgcctt tgccctcggc tatcacctcg cagctggaca aagcatccat
      901 aatcagactc acgaccagct atctcaaaat gagagtggtg ttcccagaag ggctcggcga
      961 ggcgtggggc cactcaagtc ggaccagccc cctggacaac gttggccgag aactgggctc
     1021 ccatctgctc cagaccctgg atggcttcat cttcgtggta gccccagatg ggaagatcat
     1081 gtacatctca gagacagcct cagtccactt gggtctttct caggtagagc tgaccggaaa
     1141 cagcatttat gaatacattc acccggcaga ccacgacgag atgacggcgg tgctcaccgc
     1201 ccatcaaccc taccactctc acttcgtgca ggagtatgag atcgagcgct ccttcttcct
     1261 gaggatgaag tgcgtcttgg ccaagcgtaa cgccggcctc acctgtggcg gctacaaggt
     1321 catccactgc agcggctact tgaagatccg ccagtacagc ctggacatgt cccccttcga
     1381 cggctgctac caaaacgtgg gcctggtggc cgtgggccac tcgctgcctc ccagcgccgt
     1441 cacggagatc aagctacaca gcaatatgtt tatgttccgc gccagcctgg acatgaagct
     1501 catctttctg gactccaggg tggcggagct gacggggtac gaacctcagg acctgattga
     1561 gaagactctg taccaccatg tgcacggctg cgacaccttc cacctgcgct gcgcgcacca
     1621 tttgctgctg gtgaagggac aggtgaccac caagtactac aggttcctgg cgaaacacgg
     1681 cggctgggta tgggtgcaga gctacgcgac catcgtgcac aacagtcgct cctccaggcc
     1741 acactgtatc gtcagcgtca actatgtcct cacagacaca gaatacaaag ggctgcagct
     1801 ctccctggat cagatctcag cctccaaacc agccttctcc tataccagca gctccacccc
     1861 caccatgact gacaacagaa agggggccaa atcccggctc tccagctcaa agtcaaaatc
     1921 caggacttcc ccataccctc agtattcggg atttcacaca gaaagatcgg aatctgatca
     1981 tgacagccag tggggcggaa gtcccttgac cgacacggcc tctccgcagc ttctggaccc
     2041 cgccgatagg cctggctccc agcacgacgc atcgtgcgcc tacagacagt tttcggaccg
     2101 cagctctctc tgctatggct ttgcgcttga ccactcgagg ctggtggaag agaggcattt
     2161 ccatacccag gcctgtgaag gaggccgatg tgaggcaggc aggtacttcc tgggaacgcc
     2221 gcaggccggg agggagccct ggtggggctc tcgcgcagcc ttgcccctga caaaggcctc
     2281 cccagaaagc agagaagcct atgaaaacag catgcctcac atcgcttcag tccacaggat
     2341 ccatgggcga ggtcattggg atgaagatag tgtggtcagt tctccagacc ctgggtcggc
     2401 cagtgaatca ggtgaccgat atcgtactga gcagtatcaa agtagcccac atgaacccag
     2461 caaaattgaa actcttataa gagccactca gcaaatgatt aaagaagaag agaacagatt
     2521 acagctaagg aaagccccct cagaccaact ggcttccatt aatggggctg ggaaaaaaca
     2581 ctccctgtgt tttgcaaact accaacagcc cccaccaaca ggtgaagtct gccatggctc
     2641 tgctcttgcc aacacttcac catgtgacca tatccagcag agagagggaa aaatgttgag
     2701 cccccatgaa aatgactatg acaacagtcc caccgcacta tctcggataa gtagtcccaa
     2761 ttcggatcgc atttcaaaat ccagtttgat cctagctaaa gactatctgc attcggatat
     2821 atctcctcat cagacagcag gagaccaccc tactgtctct ccaaactgct ttggctctca
     2881 ccggcagtat tttgacaagc atgcttacac attaactgga tatgccctgg agcacttata
     2941 tgacagcgaa accattagaa actattcctt gggctgtaat ggctcacact ttgatgtaac
     3001 ttcccatctg aggatgcaac cagacccagc acaaggacac aagggaacat ctgttataat
     3061 aaccaacgga agctgatgtt ttgctgaaat attttgttct ttaaggatct ctgaaacata
     3121 tttatagttt aataccccat taccagcatt tactatgcca cagattgtta gagagtataa
     3181 cttaagttac tgggtatttg atacgtgttc ctataaaatc aaagaaaaca tagcactagc
     3241 attcagggtt atacacagaa aagggagcta aattgaatac acaaatttcc cctctaatta
     3301 tatgggaacc agaatagata aattttgact tgaaaaatat tcatgtagat caagtgtgca
     3361 tatatactac atgagaggac tgatgaatga caacattgca ttgtgactat ccagtgatcc
     3421 tcaaacacac aaactattac ttacaaactg cggtatacat tttacatatg gaaatatagg
     3481 ctatgtaatg taaatacatc aaaaatgggt aattttcttt gactctgtca cactaaactt
     3541 cttaacgaaa tttccattcc caaaataact gagaaagaga gagatacatc ttataaactg
     3601 acttctttgt ggtttcaaat cagccagctc atttggttca ggcataaatt agagaaatgg
     3661 ttctggatat ggtgcaaaaa tgagttttca cctggtatcc attataaaca atcaggaaga
     3721 ggtaattttt caccttgctt ttcagttaga caaggaccag gattgcactg acatggcgct
     3781 gagggttttt ctaagtaaga acactgagat attgggacac acatcaaaaa cctggagtgc
     3841 tcaattggaa gtagttctat gaatatggaa aggccagagg cagagtgaaa taaaatgcta
     3901 tctcaaagtt taacacaatt taagggctca gcataagtaa acaacatatt tggggtttgc
     3961 ttgtaaaacc aactaaataa aaaattcaaa ccaattcacc cagaaaaaag accaataggt
     4021 gcaaaaataa aaggaaaacc agtgaagtgc cacatgacag cagtgttaag tgtttgaaaa
     4081 cgtttcaaag cacatatgtg ccaatgtgac aacatgtgga aagcctcagg agagagtcta
     4141 agataaaagc ttaggctgat agacaagtag ttaagagcta agagcagtac tctgaaggaa
     4201 taggcaaaat gtttattttc cttattgttt gtaaacaaca aacttggtct tacatctgtg
     4261 tggtatagta gaaaggccag ctgactagat ctctggattc taattttggc cctacctgta
     4321 acttaatttt gtgaccacag ttgtaccatt caccgtgcct gggctctagt ttcctggttt
     4381 gtaaggcagc cccagcgttc atgttctgtg atagagcaga actgaactta ttacctaatt
     4441 aactctctgc tatgagttgt caagactgat cattctgttt tttctgtaca cagaagttta
     4501 gatgctttgt gacttaagca ggtgtgtggg ctcctttagg caggttacag ttaactttct
     4561 agatttagcc aaaatgtagt tggtagaaat ttatgacaga gaagaaaaat agcaagattt
     4621 atttaatgct ctattctcta gtaattaaag caaaatgata taagtgatta gagagatttc
     4681 atagataata tttcctagga gtcccgtgcc caaaactctt gccatattta aggaagggaa
     4741 tgtactggca gaacttgact tacaattttg gaaacagaac atgaaattga atactcaggg
     4801 attagtagta tattttcatc atttaacaca gttatttcac agtccatagg taggtgacaa
     4861 tctgaacgat aatatgaaag atgagtactg ggttgtttgt atatgaaagg agaggctaca
     4921 taaaattaaa taatgttaac atttgtgctg taaatgcaaa aaagtgcaag taagtgagaa
     4981 agttagtggt tatatatatt ttcaaaaaat tcaaagtgca gttgaccctt gaataacatg
     5041 ggtttgaact gtgcagattc acttatgtgt ggtttttttt ccaatcaaat gcagataaaa
     5101 aacccagtat ttggagaaat gtgaaacctg agtatacaga gggcgatgtt ttgtataagt
     5161 gagcgccaca gggccaacag gcaggacttg agtatgtgag aatttgatta tatgggggta
     5221 atcctggaac caactccctg tttataccaa ggggtgacca tacaataaaa actaagtttt
     5281 gaattataca aaaaagagta tttagtaatt ttgagaaaat tatttatgag aaatcctgga
     5341 atacataaaa tggagttttg atcaatttgc aacatgaagt aatgtcttaa gaatgctaaa
     5401 ggttttgtag aattctgtag attgatagat gtttggaaga aaaaatattt gatgaatatg
     5461 gtgtattttg gaaaggtaat tttcacagat gggtatttct ggtggctata tattctacca
     5521 aatgtgccag ggacagaagc taaaagaaat ggaaacttaa gaaccaggga aaatgaactg
     5581 atattaaaga aaaggctgtc actaaaggaa tttcagtgga atttttatat ggaagatagt
     5641 taactggtga gaagaagaaa ttatgaattt cacagatgag agtaatcaga aggaataatg
     5701 ctctttaaaa tgacatgacc aatattttta agagaataca gaattaacta aattatatat
     5761 tgattcaaaa ttttggtaga ttgacccagt tttaagtaca taaccatttt aaaatttact
     5821 gaatgtcaat aattttatag tagcagtttt attacattac ataaaatgtc atattgactc
     5881 actatattgt atagcttaca ttttttgcaa tatggcatga ttatcacaaa atgacccaag
     5941 gtaagttaag acatttgatg acaatgtact ttcttaatat acatgtgaat gtgttaaaat
     6001 gtgtttataa tctacattat tttgttctgt gcagcaacat atttcctcat aatgaagatg
     6061 tcatctgtgt cttttaaaaa tagtatataa aataactacg ttcccgataa tttaatcagt
     6121 taaacttaat atggctttag ttttaattct tgatttccat aactttgcca aatgttgaga
     6181 aacatgaaac ttgattgaaa tagatacaga agtatgattt tcatatcacc ttggggatac
     6241 ataaaaatgt gctctatcca attactggta taatatgtat caattcatta gatgatacat
     6301 agctcatggt tttgtagaat tctggtttct agtgataata agtatcttta ttgcccattt
     6361 cacctgccat acctacttta aaaatttgga tgaccaaaga agtgaatgaa gtttccccaa
     6421 tctttcttgg gaaaattctg tttaaattgt tattgggaat atatagattt gatgtgttat
     6481 tttgggatgt gtaatattaa tgtcacaatc actacaggtc agatatttag gtgtgcttcg
     6541 gtccacaagt cttttgtcat attgtgctcg cttacctaaa ctgtaattgc ctgataaaag
     6601 ataatatgac agtgctgagg tgacattcaa aatgagacgg gatgcaataa atgattgcct
     6661 tcagtattta cacagaaaaa ccacttgctc tttcttggtt atatttcata gggctagatt
     6721 gtttccatcg ggcactattc ttaattcact ctataccgtg ttgtcacatg ctgcctctgg
     6781 gcagagaatc ccacttaaaa taagctttag gggatcttct agaaaagggt agtgtgctcc
     6841 aatttttatt tctccttctt atgggggatg aggatcaatt atagtacaag accatttgga
     6901 aaatatgcat atatatttta aaatgaaata caagatttcc gccctcaaga tctaaaccaa
     6961 gtaagtttga tggatggcag cacagataaa gaaatcttct gaatacacat ttgattttta
     7021 tagctctctc tttcaagagt aggatattac taagatattc agtgcaatct atcagtccta
     7081 gtagtattta gaagtgacag agtgaatgct gatatttgga aaattgagca tttgaccatt
     7141 ttggggtcac tcacctggcc tagtggaata gcttacactt taatacaatc aaacacagtc
     7201 tttctgatat aaaatgtttg aatgcccaga tttggttccc tagttcactc aaatcataga
     7261 tgttgacata catcctgttt tcataatgat tttaaaaaga atgtaaatcc tatggtcctc
     7321 caaataaatg attcaacgtt tatgtaaatg tcaagttttt gtgaataggg atttatgtct
     7381 aagacagaaa aatctactga actagttctt actgtggaac taattgtgga taaaacattc
     7441 gttatcatct aaattttaaa tgaatgacaa cagttatgga cacatgcaaa aggtatcaga
     7501 catagaaaat attgttgggg gaaatttctg tgtggtgcta atttcttgta agggttgtac
     7561 agatagtgct taactcaaaa agtgagagtg tggtcattgc caaatagggg tttgctcaga
     7621 gttccttctc taggactgta tgcagaaagc atacacacac acatacgcac acagaaaaat
     7681 atgattttta tatcatattc atgaaaatgt gccatatcca attataacac acacacacac
     7741 acacacacac acacacacac acacacataa ataaataaat gatggttcac ataagaaatc
     7801 ctaattgcta attttaaacc aaacatttgt agttttgttt attgcaactt tgctgcatgg
     7861 gactttgctt tcataaatct atatggggtt ggggttaatt tgccctaatt tgctgacctg
     7921 ggacacatgt aatcactgtt aaacttacac ccggtaaccc tgatgtgttt acatttcaaa
     7981 agaaatgaaa ttggcctgga aaaaaatttt ggaagtactg taagtctttt ttcttttttt
     8041 ttccgaaggg aaatatttca aaaaaggaaa cattatgagt agacacttca aaaaagataa
     8101 aatattttac atttgttttt tgactaatgt gctataaaaa ggattatatt tgtgagaaaa
     8161 gatactgatc gccaatattt caaataccgt cttgcaatgt atagttttta gtgacattgt
     8221 agtataaagc tgtaatttga aattttactt tggaatgtaa agtagaaaat attagctatg
     8281 tcaatgatat cttgcaaagt gttcccattt ataattattt atattgtaaa tagctttctg
     8341 aagtaaattc gaagttaatg tgcataaaat gtatttatta tgtgaggaat tttttggttt
     8401 aaaatatagt taccaatatg cactttga
//
