LOCUS       NM_014442               2949 bp    mRNA    linear   PRI 11-SEP-2024
DEFINITION  Homo sapiens sialic acid binding Ig like lectin 8 (SIGLEC8),
            transcript variant 1, mRNA.
ACCESSION   NM_014442
VERSION     NM_014442.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Li,T.A., Gonzalez-Gil,A., Awol,A.K., Ackerman,S.J., Orsburn,B.C.
            and Schnaar,R.L.
  TITLE     Sialylated keratan sulfates on MUC5B are Siglec-8 ligands in the
            human esophagus
  JOURNAL   Glycobiology 34 (10) (2024)
   PUBMED   39173029
  REMARK    GeneRIF: Sialylated keratan sulfates on MUC5B are Siglec-8 ligands
            in the human esophagus.
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Lee,H.S., Gonzalez-Gil,A., Drake,V., Li,T.A., Schnaar,R.L. and
            Kim,J.
  TITLE     Induction of the endogenous sialoglycan ligand for eosinophil death
            receptor Siglec-8 in chronic rhinosinusitis with hyperplastic nasal
            polyposis
  JOURNAL   Glycobiology 31 (8), 1026-1036 (2021)
   PUBMED   33755113
  REMARK    GeneRIF: Induction of the endogenous sialoglycan ligand for
            eosinophil death receptor Siglec-8 in chronic rhinosinusitis with
            hyperplastic nasal polyposis.
REFERENCE   3  (bases 1 to 2949)
  AUTHORS   Trebo,A., Ditsch,N., Degenhardt,T., Kuhn,C., Rahmeh,M.,
            Schmoeckel,E., Mayr,D., Czogalla,B., Kolben,T., Meister,S.,
            Mahner,S., Jeschke,U. and Hester,A.
  TITLE     First Evidence for a Role of Siglec-8 in Breast Cancer
  JOURNAL   Int J Mol Sci 22 (4), 2000 (2021)
   PUBMED   33670444
  REMARK    GeneRIF: First Evidence for a Role of Siglec-8 in Breast Cancer.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2949)
  AUTHORS   Sajay-Asbaghi,M., Sadeghi-Shabestrai,M., Monfaredan,A.,
            Seyfizadeh,N., Razavi,A. and Kazemi,T.
  TITLE     Promoter region single nucleotide polymorphism of siglec-8 gene
            associates with susceptibility to allergic asthma
  JOURNAL   Per Med 17 (3), 195-201 (2020)
   PUBMED   32077788
  REMARK    GeneRIF: Promoter region single nucleotide polymorphism of siglec-8
            gene associates with susceptibility to allergic asthma.
REFERENCE   5  (bases 1 to 2949)
  AUTHORS   Legrand,F., Cao,Y., Wechsler,J.B., Zhu,X., Zimmermann,N.,
            Rampertaap,S., Monsale,J., Romito,K., Youngblood,B.A., Brock,E.C.,
            Makiya,M.A., Tomasevic,N., Bebbington,C., Maric,I., Metcalfe,D.D.,
            Bochner,B.S. and Klion,A.D.
  TITLE     Sialic acid-binding immunoglobulin-like lectin (Siglec) 8 in
            patients with eosinophilic disorders: Receptor expression and
            targeting using chimeric antibodies
  JOURNAL   J Allergy Clin Immunol 143 (6), 2227-2237 (2019)
   PUBMED   30543818
  REMARK    GeneRIF: Siglec-8 is highly expressed on blood eosinophils from
            eosinophilic donors and normal donors and represents a potential
            therapeutic target for eosinophilic disorders
REFERENCE   6  (bases 1 to 2949)
  AUTHORS   Nutku,E., Aizawa,H., Hudson,S.A. and Bochner,B.S.
  TITLE     Ligation of Siglec-8: a selective mechanism for induction of human
            eosinophil apoptosis
  JOURNAL   Blood 101 (12), 5014-5020 (2003)
   PUBMED   12609831
  REMARK    GeneRIF: Sialic acid binding immunoglobulin-like lectin 8
            (Siglec-8) cross-linking with antibodies rapidly generated
            caspase-3-like activity and reduced eosinophil viability through
            induction of apoptosis
REFERENCE   7  (bases 1 to 2949)
  AUTHORS   Munday,J., Kerr,S., Ni,J., Cornish,A.L., Zhang,J.Q., Nicoll,G.,
            Floyd,H., Mattei,M.G., Moore,P., Liu,D. and Crocker,P.R.
  TITLE     Identification, characterization and leucocyte expression of
            Siglec-10, a novel human sialic acid-binding receptor
  JOURNAL   Biochem J 355 (Pt 2), 489-497 (2001)
   PUBMED   11284738
REFERENCE   8  (bases 1 to 2949)
  AUTHORS   Foussias,G., Yousef,G.M. and Diamandis,E.P.
  TITLE     Molecular characterization of a Siglec8 variant containing
            cytoplasmic tyrosine-based motifs, and mapping of the Siglec8 gene
  JOURNAL   Biochem Biophys Res Commun 278 (3), 775-781 (2000)
   PUBMED   11095983
REFERENCE   9  (bases 1 to 2949)
  AUTHORS   Kikly,K.K., Bochner,B.S., Freeman,S.D., Tan,K.B., Gallagher,K.T.,
            D'alessio,K.J., Holmes,S.D., Abrahamson,J.A., Erickson-Miller,C.L.,
            Murdock,P.R., Tachimoto,H., Schleimer,R.P. and White,J.R.
  TITLE     Identification of SAF-2, a novel siglec expressed on eosinophils,
            mast cells, and basophils
  JOURNAL   J Allergy Clin Immunol 105 (6 Pt 1), 1093-1100 (2000)
   PUBMED   10856141
REFERENCE   10 (bases 1 to 2949)
  AUTHORS   Floyd,H., Ni,J., Cornish,A.L., Zeng,Z., Liu,D., Carter,K.C.,
            Steel,J. and Crocker,P.R.
  TITLE     Siglec-8. A novel eosinophil-specific member of the immunoglobulin
            superfamily
  JOURNAL   J Biol Chem 275 (2), 861-866 (2000)
   PUBMED   10625619
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA388103.1, BC053319.1 and AF195092.1.
            
            On Nov 22, 2018 this sequence version replaced NM_014442.2.
            
            Summary: Sialic acid-binding immunoglobulin (Ig)-like lectins, or
            SIGLECs (e.g., CD33 (MIM 159590)), are a family of type 1
            transmembrane proteins each having a unique expression pattern,
            mostly in hemopoietic cells. SIGLEC8 is a member of the CD33-like
            subgroup of SIGLECs, which are localized to 19q13.3-q13.4 and have
            2 conserved cytoplasmic tyrosine-based motifs: an immunoreceptor
            tyrosine-based inhibitory motif, or ITIM (see MIM 604964), and a
            motif homologous to one identified in signaling lymphocyte
            activation molecule (SLAM; MIM 603492) that mediates an association
            with SLAM-associated protein (SAP; MIM 300490) (summarized by
            Foussias et al., 2000 [PubMed 11095983]).[supplied by OMIM, May
            2010].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC053319.1, SRR1803611.271576.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2159764 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000321424.7/ ENSP00000321077.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-11                DA388103.1         1-11
            12-574              BC053319.1         1-563
            575-1312            AF195092.1         565-1302
            1313-2899           BC053319.1         1302-2888
            2900-2946           AF195092.1         2832-2878
            2947-2949           BC053319.1         2936-2938
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.41"
     gene            1..2949
                     /gene="SIGLEC8"
                     /gene_synonym="SAF2; SIGLEC-8; SIGLEC8L"
                     /note="sialic acid binding Ig like lectin 8"
                     /db_xref="GeneID:27181"
                     /db_xref="HGNC:HGNC:10877"
                     /db_xref="MIM:605639"
     exon            1..521
                     /gene="SIGLEC8"
                     /gene_synonym="SAF2; SIGLEC-8; SIGLEC8L"
                     /inference="alignment:Splign:2.1.0"
     CDS             68..1567
                     /gene="SIGLEC8"
                     /gene_synonym="SAF2; SIGLEC-8; SIGLEC8L"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; SAF-2; CDw329; sialoadhesin family member 2"
                     /codon_start=1
                     /product="sialic acid-binding Ig-like lectin 8 isoform 1
                     precursor"
                     /protein_id="NP_055257.2"
                     /db_xref="CCDS:CCDS33086.1"
                     /db_xref="GeneID:27181"
                     /db_xref="HGNC:HGNC:10877"
                     /db_xref="MIM:605639"
                     /translation="MLLLLLLLPLLWGTKGMEGDRQYGDGYLLQVQELVTVQEGLCVH
                     VPCSFSYPQDGWTDSDPVHGYWFRAGDRPYQDAPVATNNPDREVQAETQGRFQLLGDI
                     WSNDCSLSIRDARKRDKGSYFFRLERGSMKWSYKSQLNYKTKQLSVFVTALTHRPDIL
                     ILGTLESGHSRNLTCSVPWACKQGTPPMISWIGASVSSPGPTTARSSVLTLTPKPQDH
                     GTSLTCQVTLPGTGVTTTSTVRLDVSYPPWNLTMTVFQGDATASTALGNGSSLSVLEG
                     QSLRLVCAVNSNPPARLSWTRGSLTLCPSRSSNPGLLELPRVHVRDEGEFTCRAQNAQ
                     GSQHISLSLSLQNEGTGTSRPVSQVTLAAVGGAGATALAFLSFCIIFIIVRSCRKKSA
                     RPAAGVGDTGMEDAKAIRGSASQGPLTESWKDGNPLKKPPPAVAPSSGEEGELHYATL
                     SFHKVKPQDPQGQEATDSEYSEIKIHKRETAETQACLRNHNPSSKEVRG"
     exon            522..800
                     /gene="SIGLEC8"
                     /gene_synonym="SAF2; SIGLEC-8; SIGLEC8L"
                     /inference="alignment:Splign:2.1.0"
     exon            801..848
                     /gene="SIGLEC8"
                     /gene_synonym="SAF2; SIGLEC-8; SIGLEC8L"
                     /inference="alignment:Splign:2.1.0"
     exon            849..1118
                     /gene="SIGLEC8"
                     /gene_synonym="SAF2; SIGLEC-8; SIGLEC8L"
                     /inference="alignment:Splign:2.1.0"
     exon            1119..1215
                     /gene="SIGLEC8"
                     /gene_synonym="SAF2; SIGLEC-8; SIGLEC8L"
                     /inference="alignment:Splign:2.1.0"
     exon            1216..1312
                     /gene="SIGLEC8"
                     /gene_synonym="SAF2; SIGLEC-8; SIGLEC8L"
                     /inference="alignment:Splign:2.1.0"
     exon            1313..2949
                     /gene="SIGLEC8"
                     /gene_synonym="SAF2; SIGLEC-8; SIGLEC8L"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2924..2929
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SIGLEC8"
                     /gene_synonym="SAF2; SIGLEC-8; SIGLEC8L"
                     /note="hexamer: ATTACA"
     polyA_site      2949
                     /gene="SIGLEC8"
                     /gene_synonym="SAF2; SIGLEC-8; SIGLEC8L"
                     /note="major polyA site"
ORIGIN      
        1 agtttctgag agaagaaccc tgaggaacag acgttccctg gcggccctgg cgccttcaaa
       61 cccagacatg ctgctgctgc tgctgctgct gcccctgctc tgggggacaa aggggatgga
      121 gggagacaga caatatgggg atggttactt gctgcaagtg caggagctgg tgacggtgca
      181 ggagggcctg tgtgtccatg tgccctgctc cttctcctac ccccaggatg gctggactga
      241 ctctgaccca gttcatggct actggttccg ggcaggagac agaccatacc aagacgctcc
      301 agtggccaca aacaacccag acagagaagt gcaggcagag acccagggcc gattccaact
      361 ccttggggac atttggagca acgactgctc cctgagcatc agagacgcca ggaagaggga
      421 taaggggtca tatttctttc ggctagagag aggaagcatg aaatggagtt acaaatcaca
      481 gttgaattac aaaactaagc agctgtctgt gtttgtgaca gccctgaccc ataggcctga
      541 catcctcatc ctagggaccc tagagtctgg ccactccagg aacctgacct gctctgtgcc
      601 ctgggcctgt aagcagggga caccccccat gatctcctgg attggggcct ccgtgtcctc
      661 cccgggcccc actactgccc gctcctcagt gctcaccctt accccaaagc cccaggacca
      721 cggcaccagc ctcacctgtc aggtgacctt gcctgggaca ggtgtgacca cgaccagtac
      781 cgtccgcctc gatgtgtcct accctccttg gaacttgacc atgactgtct tccaaggaga
      841 tgccacagca tccacagccc tgggaaatgg ctcatctctt tcagtccttg agggccagtc
      901 tctgcgcctg gtctgtgctg tcaacagcaa tccccctgcc aggctgagct ggacccgggg
      961 gagcctgacc ctgtgcccct cacggtcctc aaaccctggg ctgctggagc tgcctcgagt
     1021 gcacgtgagg gatgaagggg aattcacctg ccgagctcag aacgctcagg gctcccagca
     1081 catttccctg agcctctccc tgcagaatga gggcacaggc acctcaagac ctgtatcaca
     1141 agtgacactg gcagcagtcg ggggagctgg agccacagcc ctggccttcc tgtccttctg
     1201 catcatcttc atcatagtga ggtcctgcag gaagaaatcg gcaaggccag cagcgggcgt
     1261 gggggataca ggcatggaag atgcaaaggc catcaggggc tcggcctctc agggacccct
     1321 gactgaatcc tggaaagatg gcaaccccct gaagaagcct cccccagctg ttgccccctc
     1381 gtcaggggag gaaggagagc tccattatgc aaccctcagc ttccataaag tgaagcctca
     1441 ggacccgcag ggacaggagg ccactgacag tgaatactcg gagatcaaga tccacaagcg
     1501 agaaactgca gagactcagg cctgtttgag gaatcacaac ccctccagca aagaagtcag
     1561 aggctgattc tcatagaaca agaaccctct agagccccat gctatgcagt aggtcaccag
     1621 ggctccctcc tcctgtctaa ccaaaacttg gaccaatgtc tcccctttcc ccggctacca
     1681 gggacccatc cctgcctcta gcttctacta cccaccattc tcctctcgac ctctctgagg
     1741 ttgactattt tagattccac atagagatga ggtcatgtgg tacttgcctc tctgtgtgtg
     1801 gctcatttta cacaaaaaaa tatcccctag gttcatccat gttctctcaa atgacagaat
     1861 caagcactga atattttttt ttctttgaga gatggagttt cgctctgttg cccaggctgg
     1921 agtgcagtgg ttcaatctct gctcactgca acctccacct cctgggttca aacgattctc
     1981 ctgcctcagc ttcccaagta gctggtacta caggcgtgtg tcaccacgcc cagctaattt
     2041 ttgtattttt tagtagagac ggggtttcac tataagtggg ccaggctagt ctcaaactcc
     2101 tgacctcaag tgatctgcct gccttggcct cccaaagtgc tgggatttca ggcatgagcc
     2161 accgcaccca gcttgcattg aatattttca aggagctaaa agaagatttt aaatggtctc
     2221 acaaaaacag ataaatattt gcacagatgg gtgtgctaat cattgtgcct tgatggttcc
     2281 acgatgtatc cgggtgtgga aatctcactg ggtctctctc aaggccactc ggctactcag
     2341 gacagggctg gaatttaaag cctgtccgat tctgaggtct cttctctcat ctagcactga
     2401 gtcaagcaat cagcaggctg ggcacccctt agccataagt tttcaggaaa taaattcctt
     2461 gagggcattg acttttacaa aagagggagc agcaatggcc tagagtctca ggaacaagac
     2521 aggtgcactg aggagatgaa ggccgggacc ccctgcccaa cctgtatggc gggtctgtac
     2581 ttattttgtt tacccccaat ttaaaacgtt tttttttatt gcaggttgtt tgtttgatat
     2641 ggtttggctg tgtccccacc caaatcttat ctagaattgt aatcagaatt ataatcccca
     2701 tgtgttgggg gagggacctg gtgggaggtg ataggatcat gggggtggtt cccccatgct
     2761 gttctgatag tgagtgagtt atcacgagat ctgatggttt tgtaagtggt ggtttcccct
     2821 gctcttctct cttgcctgcc accatgtaag atgtgcctgg ttccccttcc gccatgattg
     2881 taagtttcct gaggcctccc ccgccatgtg gaactgtgag tcaattacac ctctttcatt
     2941 tataaatta
//
