LOCUS       NM_023068               6960 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens sialic acid binding Ig like lectin 1 (SIGLEC1),
            transcript variant 1, mRNA.
ACCESSION   NM_023068 XM_011529325
VERSION     NM_023068.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6960)
  AUTHORS   Guo,M., Guo,H., Zhu,J., Wang,F., Chen,J., Wan,C., Deng,Y., Wang,F.,
            Xu,L., Chen,Y., Li,R., Liu,S., Zhang,L., Wang,Y., Zhou,J. and Li,S.
  TITLE     A novel subpopulation of monocytes with a strong interferon
            signature indicated by SIGLEC-1 is present in patients with in
            recent-onset type 1 diabetes
  JOURNAL   Diabetologia 67 (4), 623-640 (2024)
   PUBMED   38349399
  REMARK    GeneRIF: A novel subpopulation of monocytes with a strong
            interferon signature indicated by SIGLEC-1 is present in patients
            with in recent-onset type 1 diabetes.
REFERENCE   2  (bases 1 to 6960)
  AUTHORS   Sakumura,N., Yokoyama,T., Usami,M., Hosono,Y., Inoue,N.,
            Matsuda,Y., Tasaki,Y. and Wada,T.
  TITLE     CD169 expression on monocytes as a marker for assessing type I
            interferon status in pediatric inflammatory diseases
  JOURNAL   Clin Immunol 250, 109329 (2023)
   PUBMED   37061149
  REMARK    GeneRIF: CD169 expression on monocytes as a marker for assessing
            type I interferon status in pediatric inflammatory diseases.
REFERENCE   3  (bases 1 to 6960)
  AUTHORS   Klaas,M., Dubock,S., Ferguson,D.J.P. and Crocker,P.R.
  TITLE     Sialoadhesin (CD169/Siglec-1) is an extended molecule that escapes
            inhibitory cis-interactions and synergizes with other macrophage
            receptors to promote phagocytosis
  JOURNAL   Glycoconj J 40 (2), 213-223 (2023)
   PUBMED   36738392
  REMARK    GeneRIF: Sialoadhesin (CD169/Siglec-1) is an extended molecule that
            escapes inhibitory cis-interactions and synergizes with other
            macrophage receptors to promote phagocytosis.
REFERENCE   4  (bases 1 to 6960)
  AUTHORS   Jalloh,S., Olejnik,J., Berrigan,J., Nisa,A., Suder,E.L.,
            Akiyama,H., Lei,M., Ramaswamy,S., Tyagi,S., Bushkin,Y.,
            Muhlberger,E. and Gummuluru,S.
  TITLE     CD169-mediated restrictive SARS-CoV-2 infection of macrophages
            induces pro-inflammatory responses
  JOURNAL   PLoS Pathog 18 (10), e1010479 (2022)
   PUBMED   36279285
  REMARK    GeneRIF: CD169-mediated restrictive SARS-CoV-2 infection of
            macrophages induces pro-inflammatory responses.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6960)
  AUTHORS   Wang,Y., Jin,Z., Sun,J., Chen,X., Xie,P., Zhou,Y. and Wang,S.
  TITLE     The role of activated monocyte IFN/SIGLEC1 signalling in Graves'
            disease
  JOURNAL   J Endocrinol 255 (1), 1-9 (2022)
   PUBMED   35695299
  REMARK    GeneRIF: The role of activated monocyte IFN/SIGLEC1 signalling in
            Graves' disease.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6960)
  AUTHORS   Hartnell,A., Steel,J., Turley,H., Jones,M., Jackson,D.G. and
            Crocker,P.R.
  TITLE     Characterization of human sialoadhesin, a sialic acid binding
            receptor expressed by resident and inflammatory macrophage
            populations
  JOURNAL   Blood 97 (1), 288-296 (2001)
   PUBMED   11133773
REFERENCE   7  (bases 1 to 6960)
  AUTHORS   Hattori,A., Okumura,K., Nagase,T., Kikuno,R., Hirosawa,M. and
            Ohara,O.
  TITLE     Characterization of long cDNA clones from human adult spleen
  JOURNAL   DNA Res 7 (6), 357-366 (2000)
   PUBMED   11214971
REFERENCE   8  (bases 1 to 6960)
  AUTHORS   Nath,D., Hartnell,A., Happerfield,L., Miles,D.W., Burchell,J.,
            Taylor-Papadimitriou,J. and Crocker,P.R.
  TITLE     Macrophage-tumour cell interactions: identification of MUC1 on
            breast cancer cells as a potential counter-receptor for the
            macrophage-restricted receptor, sialoadhesin
  JOURNAL   Immunology 98 (2), 213-219 (1999)
   PUBMED   10610356
REFERENCE   9  (bases 1 to 6960)
  AUTHORS   Zamze,S., Harvey,D.J., Pesheva,P., Mattu,T.S., Schachner,M.,
            Dwek,R.A. and Wing,D.R.
  TITLE     Glycosylation of a CNS-specific extracellular matrix glycoprotein,
            tenascin-R, is dominated by O-linked sialylated glycans and
            'brain-type' neutral N-glycans
  JOURNAL   Glycobiology 9 (8), 823-831 (1999)
   PUBMED   10406848
REFERENCE   10 (bases 1 to 6960)
  AUTHORS   Mucklow,S., Hartnell,A., Mattei,M.G., Gordon,S. and Crocker,P.R.
  TITLE     Sialoadhesin (Sn) maps to mouse chromosome 2 and human chromosome
            20 and is not linked to the other members of the sialoadhesin
            family, CD22, MAG, and CD33
  JOURNAL   Genomics 28 (2), 344-346 (1995)
   PUBMED   8530048
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL109804.41, AF230073.1,
            AY927519.1, AK024462.1 and CD365566.1.
            
            On or before Nov 7, 2018 this sequence version replaced
            XM_011529325.2, NM_023068.3.
            
            Summary: This gene encodes a member of the immunoglobulin
            superfamily. The encoded protein is a lectin-like adhesion molecule
            that binds glycoconjugate ligands on cell surfaces in a sialic
            acid-dependent manner. It is a type I transmembrane protein
            expressed only by a subpopulation of macrophages and is involved in
            mediating cell-cell interactions. The protein plays an important
            role in multiple human diseases and bacterial and viral infections
            has been shown to enhance SARS-CoV-2 infection. [provided by
            RefSeq, Dec 2021].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            CDS exon combination :: AF230073.1 [ECO:0000331]
            RNAseq introns       :: mixed sample support SAMEA1965299,
                                    SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            coronavirus related       :: involved in SARS-CoV-2 infection
            inferred exon combination :: based on alignments, homology
            MANE Ensembl match        :: ENST00000344754.6/ ENSP00000341141.4
            RefSeq Select criteria    :: based on manual assertion,
                                         conservation, expression, longest
                                         protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-131               AL109804.41        37056-37186         c
            132-240             AL109804.41        31715-31823         c
            241-593             AF230073.1         1-353
            594-776             AY927519.1         262-444
            777-4284            AF230073.1         537-4044
            4285-6957           AK024462.1         1849-4521
            6958-6960           CD365566.1         17-19               c
FEATURES             Location/Qualifiers
     source          1..6960
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20p13"
     gene            1..6960
                     /gene="SIGLEC1"
                     /gene_synonym="CD169; SIGLEC-1; SN"
                     /note="sialic acid binding Ig like lectin 1"
                     /db_xref="GeneID:6614"
                     /db_xref="HGNC:HGNC:11127"
                     /db_xref="MIM:600751"
     exon            1..131
                     /gene="SIGLEC1"
                     /gene_synonym="CD169; SIGLEC-1; SN"
                     /inference="alignment:Splign:2.1.0"
     exon            132..289
                     /gene="SIGLEC1"
                     /gene_synonym="CD169; SIGLEC-1; SN"
                     /inference="alignment:Splign:2.1.0"
     CDS             241..5370
                     /gene="SIGLEC1"
                     /gene_synonym="CD169; SIGLEC-1; SN"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; sialic acid-binding immunoglobulin-like lectin
                     1; sialoadhesin; sialic acid binding Ig-like lectin 1,
                     sialoadhesin"
                     /codon_start=1
                     /product="sialoadhesin isoform 1 precursor"
                     /protein_id="NP_075556.1"
                     /db_xref="CCDS:CCDS13060.1"
                     /db_xref="GeneID:6614"
                     /db_xref="HGNC:HGNC:11127"
                     /db_xref="MIM:600751"
                     /translation="MGFLPKLLLLASFFPAGQASWGVSSPQDVQGVKGSCLLIPCIFS
                     FPADVEVPDGITAIWYYDYSGQRQVVSHSADPKLVEARFRGRTEFMGNPEHRVCNLLL
                     KDLQPEDSGSYNFRFEISEVNRWSDVKGTLVTVTEEPRVPTIASPVELLEGTEVDFNC
                     STPYVCLQEQVRLQWQGQDPARSVTFNSQKFEPTGVGHLETLHMAMSWQDHGRILRCQ
                     LSVANHRAQSEIHLQVKYAPKGVKILLSPSGRNILPGELVTLTCQVNSSYPAVSSIKW
                     LKDGVRLQTKTGVLHLPQAAWSDAGVYTCQAENGVGSLVSPPISLHIFMAEVQVSPAG
                     PILENQTVTLVCNTPNEAPSDLRYSWYKNHVLLEDAHSHTLRLHLATRADTGFYFCEV
                     QNVHGSERSGPVSVVVNHPPLTPVLTAFLETQAGLVGILHCSVVSEPLATLVLSHGGH
                     ILASTSGDSDHSPRFSGTSGPNSLRLEIRDLEETDSGEYKCSATNSLGNATSTLDFHA
                     NAARLLISPAAEVVEGQAVTLSCRSGLSPTPDARFSWYLNGALLHEGPGSSLLLPAAS
                     STDAGSYHCRARDGHSASGPSSPAVLTVLYPPRQPTFTTRLDLDAAGAGAGRRGLLLC
                     RVDSDPPARLQLLHKDRVVATSLPSGGGCSTCGGCSPRMKVTKAPNLLRVEIHNPLLE
                     EEGLYLCEASNALGNASTSATFNGQATVLAIAPSHTLQEGTEANLTCNVSREAAGSPA
                     NFSWFRNGVLWAQGPLETVTLLPVARTDAALYACRILTEAGAQLSTPVLLSVLYPPDR
                     PKLSALLDMGQGHMALFICTVDSRPLALLALFHGEHLLATSLGPQVPSHGRFQAKAEA
                     NSLKLEVRELGLGDSGSYRCEATNVLGSSNTSLFFQVRGAWVQVSPSPELQEGQAVVL
                     SCQVHTGVPEGTSYRWYRDGQPLQESTSATLRFAAITLTQAGAYHCQAQAPGSATTSL
                     AAPISLHVSYAPRHVTLTTLMDTGPGRLGLLLCRVDSDPPAQLRLLHGDRLVASTLQG
                     VGGPEGSSPRLHVAVAPNTLRLEIHGAMLEDEGVYICEASNTLGQASASADFDAQAVN
                     VQVWPGATVREGQLVNLTCLVWTTHPAQLTYTWYQDGQQRLDAHSIPLPNVTVRDATS
                     YRCGVGPPGRAPRLSRPITLDVLYAPRNLRLTYLLESHGGQLALVLCTVDSRPPAQLA
                     LSHAGRLLASSTAASVPNTLRLELRGPQPRDEGFYSCSARSPLGQANTSLELRLEGVR
                     VILAPEAAVPEGAPITVTCADPAAHAPTLYTWYHNGRWLQEGPAASLSFLVATRAHAG
                     AYSCQAQDAQGTRSSRPAALQVLYAPQDAVLSSFRDSRARSMAVIQCTVDSEPPAELA
                     LSHDGKVLATSSGVHSLASGTGHVQVARNALRLQVQDVPAGDDTYVCTAQNLLGSIST
                     IGRLQVEGARVVAEPGLDVPEGAALNLSCRLLGGPGPVGNSTFAWFWNDRRLHAEPVP
                     TLAFTHVARAQAGMYHCLAELPTGAAASAPVMLRVLYPPKTPTMMVFVEPEGGLRGIL
                     DCRVDSEPLASLTLHLGSRLVASSQPQGAPAEPHIHVLASPNALRVDIEALRPSDQGE
                     YICSASNVLGSASTSTYFGVRALHRLHQFQQLLWVLGLLVGLLLLLLGLGACYTWRRR
                     RVCKQSMGENSVEMAFQKETTQLIDPDAATCETSTCAPPLG"
     exon            290..649
                     /gene="SIGLEC1"
                     /gene_synonym="CD169; SIGLEC-1; SN"
                     /inference="alignment:Splign:2.1.0"
     exon            650..946
                     /gene="SIGLEC1"
                     /gene_synonym="CD169; SIGLEC-1; SN"
                     /inference="alignment:Splign:2.1.0"
     exon            947..1213
                     /gene="SIGLEC1"
                     /gene_synonym="CD169; SIGLEC-1; SN"
                     /inference="alignment:Splign:2.1.0"
     exon            1214..1468
                     /gene="SIGLEC1"
                     /gene_synonym="CD169; SIGLEC-1; SN"
                     /inference="alignment:Splign:2.1.0"
     exon            1469..1768
                     /gene="SIGLEC1"
                     /gene_synonym="CD169; SIGLEC-1; SN"
                     /inference="alignment:Splign:2.1.0"
     exon            1769..2026
                     /gene="SIGLEC1"
                     /gene_synonym="CD169; SIGLEC-1; SN"
                     /inference="alignment:Splign:2.1.0"
     exon            2027..2362
                     /gene="SIGLEC1"
                     /gene_synonym="CD169; SIGLEC-1; SN"
                     /inference="alignment:Splign:2.1.0"
     exon            2363..2620
                     /gene="SIGLEC1"
                     /gene_synonym="CD169; SIGLEC-1; SN"
                     /inference="alignment:Splign:2.1.0"
     exon            2621..2923
                     /gene="SIGLEC1"
                     /gene_synonym="CD169; SIGLEC-1; SN"
                     /inference="alignment:Splign:2.1.0"
     exon            2924..3184
                     /gene="SIGLEC1"
                     /gene_synonym="CD169; SIGLEC-1; SN"
                     /inference="alignment:Splign:2.1.0"
     exon            3185..3496
                     /gene="SIGLEC1"
                     /gene_synonym="CD169; SIGLEC-1; SN"
                     /inference="alignment:Splign:2.1.0"
     exon            3497..3748
                     /gene="SIGLEC1"
                     /gene_synonym="CD169; SIGLEC-1; SN"
                     /inference="alignment:Splign:2.1.0"
     exon            3749..4018
                     /gene="SIGLEC1"
                     /gene_synonym="CD169; SIGLEC-1; SN"
                     /inference="alignment:Splign:2.1.0"
     exon            4019..4270
                     /gene="SIGLEC1"
                     /gene_synonym="CD169; SIGLEC-1; SN"
                     /inference="alignment:Splign:2.1.0"
     exon            4271..4570
                     /gene="SIGLEC1"
                     /gene_synonym="CD169; SIGLEC-1; SN"
                     /inference="alignment:Splign:2.1.0"
     exon            4571..4831
                     /gene="SIGLEC1"
                     /gene_synonym="CD169; SIGLEC-1; SN"
                     /inference="alignment:Splign:2.1.0"
     exon            4832..5134
                     /gene="SIGLEC1"
                     /gene_synonym="CD169; SIGLEC-1; SN"
                     /inference="alignment:Splign:2.1.0"
     exon            5135..5237
                     /gene="SIGLEC1"
                     /gene_synonym="CD169; SIGLEC-1; SN"
                     /inference="alignment:Splign:2.1.0"
     exon            5238..5310
                     /gene="SIGLEC1"
                     /gene_synonym="CD169; SIGLEC-1; SN"
                     /inference="alignment:Splign:2.1.0"
     exon            5311..6960
                     /gene="SIGLEC1"
                     /gene_synonym="CD169; SIGLEC-1; SN"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6936..6941
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SIGLEC1"
                     /gene_synonym="CD169; SIGLEC-1; SN"
                     /note="hexamer: AATAAA"
     polyA_site      6960
                     /gene="SIGLEC1"
                     /gene_synonym="CD169; SIGLEC-1; SN"
                     /note="major polyA site"
ORIGIN      
        1 gatacgggat ccttctctct tgagccggca gagccctggg gagccagctg caccaggagc
       61 tcttggccgt ccttcctgtt ctcctctccg caggtccagc accgccgcag ccacttcctt
      121 ctgtggagca gcaggtgctg gggctaaagt gcccagaggt gtccctggag gcccctgtga
      181 gcccagccag cgcagtgcgc accaccctct taggcaacag gagcagcaca agaacctgct
      241 atgggcttct tgcccaagct tctcctcctg gcctcattct tcccagcagg ccaggcctca
      301 tggggcgtct ccagtcccca ggacgtgcag ggtgtgaagg ggtcttgcct gcttatcccc
      361 tgcatcttca gcttccctgc cgacgtggag gtgcccgacg gcatcacggc catctggtac
      421 tacgactact cgggccagcg gcaggtggtg agccactcgg cggaccccaa gctggtggag
      481 gcccgcttcc gcggccgcac cgagttcatg gggaaccccg agcacagggt gtgcaacctg
      541 ctgctgaagg acctgcagcc cgaggactct ggttcctaca acttccgctt cgagatcagt
      601 gaggtcaacc gctggtcaga tgtgaaaggc accttggtca cagtaacaga ggagcccagg
      661 gtgcccacca ttgcctcccc ggtggagctt ctcgagggca cagaggtgga cttcaactgc
      721 tccactccct acgtatgcct gcaggagcag gtcagactgc agtggcaagg ccaggaccct
      781 gctcgctctg tcaccttcaa cagccagaag tttgagccca ccggcgtcgg ccacctggag
      841 accctccaca tggccatgtc ctggcaggac cacggccgga tcctgcgctg ccagctctcc
      901 gtggccaatc acagggctca gagcgagatt cacctccaag tgaagtatgc ccccaagggt
      961 gtgaagatcc tcctcagccc ctcggggagg aacatccttc caggtgagct ggtcacactc
     1021 acctgccagg tgaacagcag ctaccctgca gtcagttcca ttaagtggct caaggatggg
     1081 gtacgcctcc aaaccaagac tggtgtgctg cacctgcccc aggcagcctg gagcgatgct
     1141 ggcgtctaca cctgccaagc tgagaacggc gtgggctctt tggtctcacc ccccatcagc
     1201 ctccacatct tcatggctga ggtccaggtg agcccagcag gtcccatcct ggagaaccag
     1261 acagtgacac tagtctgcaa cacacccaat gaggcaccca gtgatctccg ctacagctgg
     1321 tacaagaacc atgtcctgct ggaggatgcc cactcccata ccctccggct gcacttggcc
     1381 actagggctg atactggctt ctacttctgt gaggtgcaga acgtccatgg cagcgagcgc
     1441 tcgggccctg tcagcgtggt agtcaaccac ccgcctctca ctccagtcct gacagccttc
     1501 ctggagaccc aggcgggact tgtgggcatc cttcactgct ctgtggtcag tgagcccctg
     1561 gccacactgg tgctgtcaca tgggggtcat atcctggcct ccacctccgg ggacagtgat
     1621 cacagcccac gcttcagtgg tacctctggt cccaactccc tgcgcctgga gatccgagac
     1681 ctggaggaaa ctgacagtgg ggagtacaag tgctcagcca ccaactccct tggaaatgca
     1741 acctccaccc tggacttcca tgccaatgcc gcccgtctcc tcatcagccc ggcagccgag
     1801 gtggtggaag gacaggcagt gacactgagc tgcagaagcg gcctaagccc cacacctgat
     1861 gcccgcttct cctggtacct gaatggagcc ctgcttcacg agggtcccgg cagcagcctc
     1921 ctgctccccg cggcctccag cactgacgcc ggctcatacc actgccgggc ccgggacggc
     1981 cacagtgcca gtggcccctc ttcgccagct gttctcactg tgctctaccc ccctcgacaa
     2041 ccaacattca ccaccaggct ggaccttgat gccgctgggg ccggggctgg acggcgaggc
     2101 ctccttttgt gccgtgtgga cagcgacccc cccgccaggc tgcagctgct ccacaaggac
     2161 cgtgttgtgg ccacttccct gccatcaggg ggtggctgca gcacctgtgg gggctgttcc
     2221 ccacgcatga aggtcaccaa agcccccaac ttgctgcgtg tggagattca caaccctttg
     2281 ctggaagagg agggcttgta cctctgtgag gccagcaatg ccctgggcaa cgcctccacc
     2341 tcagccacct tcaatggcca ggccactgtc ctggccattg caccatcaca cacacttcag
     2401 gagggcacag aagccaactt gacttgcaac gtgagccggg aagctgctgg cagccctgct
     2461 aacttctcct ggttccgaaa tggggtgctg tgggcccagg gtcccctgga gaccgtgaca
     2521 ctgctgcccg tggccagaac tgatgctgcc ctttacgcct gccgcatcct gactgaggct
     2581 ggtgcccagc tctccactcc cgtgctcctg agtgtactct atcccccgga ccgtccaaag
     2641 ctgtcagccc tcctagacat gggccagggc cacatggctc tgttcatctg cactgtggac
     2701 agccgccccc tggccttgct ggccttgttc catggggagc acctcctggc caccagcctg
     2761 ggtccccagg tcccatccca tggtcggttc caggctaaag ctgaggccaa ctccctgaag
     2821 ttagaggtcc gagaactggg ccttggggac tctggcagct accgctgtga ggccacaaat
     2881 gttcttggat catccaacac ctcactcttc ttccaggtcc gaggagcctg ggtccaggtg
     2941 tcaccatcac ctgagctcca agagggccag gctgtggtcc tgagctgcca ggtacacaca
     3001 ggagtcccag aggggacctc atatcgttgg tatcgggatg gccagcccct ccaggagtcg
     3061 acctcggcca cgctccgctt tgcagccata actttgacac aagctggggc ctatcattgc
     3121 caagcccagg ccccaggctc agccaccacg agcctagctg cacccatcag cctccacgtg
     3181 tcctatgccc cacgccacgt cacactcact accctgatgg acacaggccc tggacgactg
     3241 ggcctcctcc tgtgccgtgt ggacagtgac cctccggccc agctgcggct gctccacggg
     3301 gatcgccttg tggcctccac cctacaaggt gtggggggac ccgaaggcag ctctcccagg
     3361 ctgcatgtgg ctgtggcccc caacacactg cgtctggaga tccacggggc tatgctggag
     3421 gatgagggtg tctatatctg tgaggcctcc aacaccctgg gccaggcctc ggcctcagct
     3481 gacttcgacg ctcaagctgt gaatgtgcag gtgtggcccg gggctaccgt gcgggagggg
     3541 cagctggtga acctgacctg ccttgtgtgg accactcacc cggcccagct cacctacaca
     3601 tggtaccagg atgggcagca gcgcctggat gcccactcca tccccctgcc caacgtcaca
     3661 gtcagggatg ccacctccta ccgctgcggt gtgggccccc ctggtcgggc accccgcctc
     3721 tccagaccta tcaccttgga cgtcctctac gcgccccgca acctgcgcct gacctacctc
     3781 ctggagagcc atggcgggca gctggccctg gtactgtgca ctgtggacag ccgcccgccc
     3841 gcccagctgg ccctcagcca cgccggtcgc ctcttggcct cctcgacagc agcctctgtc
     3901 cccaacaccc tgcgcctgga gctgcgaggg ccacagccca gggatgaggg tttctacagc
     3961 tgctctgccc gcagccctct gggccaggcc aacacgtccc tggagctgcg gctggagggt
     4021 gtgcgggtga tcctggctcc ggaggctgcc gtgcctgaag gtgcccccat cacagtgacc
     4081 tgtgcggacc ctgctgccca cgcacccaca ctctatactt ggtaccacaa cggtcgttgg
     4141 ctgcaggagg gtccagctgc ctcactctca ttcctggtgg ccacgcgggc tcatgcaggc
     4201 gcctactctt gccaggccca ggatgcccag ggcacccgca gctcccgtcc tgctgccctg
     4261 caagtcctct atgcccctca ggacgctgtc ctgtcctcct tccgggactc cagggccaga
     4321 tccatggctg tgatacagtg cactgtggac agtgagccac ctgctgagct ggccctatct
     4381 catgatggca aggtgctggc cacgagcagc ggggtccaca gcttggcatc agggacaggc
     4441 catgtccagg tggcccgaaa cgccctacgg ctgcaggtgc aagatgtgcc tgcaggtgat
     4501 gacacctatg tttgcacagc ccaaaacttg ctgggctcaa tcagcaccat cgggcggttg
     4561 caggtagaag gtgcacgcgt ggtggcagag cctggcctgg acgtgcctga gggcgctgcc
     4621 ctgaacctca gctgccgcct cctgggtggc cctgggcctg tgggcaactc cacctttgca
     4681 tggttctgga atgaccggcg gctgcacgcg gagcctgtgc ccactctcgc cttcacccac
     4741 gtggctcgtg ctcaagctgg gatgtaccac tgcctggctg agctccccac tggggctgct
     4801 gcctctgctc cagtcatgct ccgtgtgctc taccctccca agacgcccac catgatggtc
     4861 ttcgtggagc ctgagggtgg cctccggggc atcctggatt gccgagtgga cagcgagccg
     4921 ctcgccagcc tgactctcca ccttggcagt cgactggtgg cctccagtca gccccagggt
     4981 gctcctgcag agccacacat ccatgtcctg gcttccccca atgccctgag ggtggacatc
     5041 gaggcgctga ggcccagcga ccaaggggaa tacatctgtt ctgcctcaaa tgtcctgggc
     5101 tctgcctcta cctccaccta ctttggggtc agagccctgc accgcctgca tcagttccag
     5161 cagctgctct gggtcctggg actgctggtg ggcctcctgc tcctgctgtt gggcctgggg
     5221 gcctgctaca cctggagaag gaggcgtgtt tgtaagcaga gcatgggcga gaattcggtg
     5281 gagatggctt ttcagaaaga gaccacgcag ctcattgatc ctgatgcagc cacatgtgag
     5341 acctcaacct gtgccccacc cctgggctga ccagtggtgt tgcctgccct ccggaggaga
     5401 aagtggccag aatctgtgat gactccagcc tatgaatgtg aatgaggcag tgttgagtcc
     5461 tgcccgcctc tacgaaaaca gctctgtgac atctgacttt ttatgacctg gccccaagcc
     5521 tcttgccccc ccaaaaatgg gtggtgagag gtctgcccag gagggtgttg accctggagg
     5581 acactgaaga gcactgagct gatctcgctc tctcttctct ggatctcctc ccttctctcc
     5641 atttctccct caaaggaagc cctgcccttt cacatccttc tcctcgaaag tcaccctgga
     5701 ctttggttgg attgcagcat cctgcatcct cagaggctca ccaaggcatt ctgtattcaa
     5761 cagagtatca gtcagcctgc tctaacaaga gaccaaatac agtgacttca acatgataga
     5821 attttatttt tctctcccac gctagtctgg ctgttacgat ggtttatgat gttggggctc
     5881 aggatccttc tatcttcctt ttctctatcc ctaaaatgat gcctttgatt gtgaggctca
     5941 ccatggcccc gctttgtcca catgccctcc agccagaaga aggaagagtg gaggtagaag
     6001 cacacccatg cccatggtgg acgcaactca gaagctgcac aggacttttc cactcacttc
     6061 ccattggctg gagtattgtc acatggctac tgcaagctac aagggagact gggaaatgta
     6121 gtttttattt tgagtccaga ggacatttgg aattggactt ccaaaggact cccaactgtg
     6181 agctcatccc tgagactttt gacattgttg ggaatgccac cagcaggcca tgttttgtct
     6241 cagtgcccat ctactgaggg ccagggtgtg cccctggcca ttctggttgt gggcttcctg
     6301 gaagaggtga tcactctcac actaagactg aggaaataaa aaaggtttgg tgttttccta
     6361 gggagagagc atgccaggca gtggagttgc ctaagcagac atccttgtgc cagatttggc
     6421 ccctgaaaga agagatgccc tcattcccac caccaccccc cctaccccca gggactgggt
     6481 actaccttac tggcccttac aagagtggag ggcagacaca gatgttgtca gcatccttat
     6541 tcctgctcca gatgcatctc tgttcatgac tgtgtgagct cctgtccttt tcctggagac
     6601 cctgtgtcgg gctgttaaag agaatgagtt accaagaagg aatgacgtgc ccctgcgaat
     6661 cagggaccaa caggagagag ctcttgagtg ggctagtgac tccccctgca gcctggtgga
     6721 gatggtgtga ggagcgaaga gccctctgct ctaggatttg ggttgaaaaa cagagagaga
     6781 agtggggagt tgccacagga gctaacacgc tgggaggcag ttgggggcgg gtgaactttg
     6841 tgtagccgag gccgcaccct ccctcattcc aggctcattc attttcatgc tccattgcca
     6901 gactcttgct gggagcccgt ccagaatgtc ctcccaataa aactccatcc tatgacgcaa
//
