LOCUS       NM_020717              14557 bp    mRNA    linear   PRI 12-JUN-2024
DEFINITION  Homo sapiens shroom family member 4 (SHROOM4), transcript variant
            1, mRNA.
ACCESSION   NM_020717 XM_050478
VERSION     NM_020717.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 14557)
  AUTHORS   Kolvenbach,C.M., Felger,T., Schierbaum,L., Thiffault,I.,
            Pastinen,T., Szczepanska,M., Zaniew,M., Adamczyk,P., Bayat,A.,
            Yilmaz,O., Lindenberg,T.T., Thiele,H., Hildebrandt,F.,
            Hinderhofer,K., Moog,U., Hilger,A.C., Sullivan,B., Bartik,L.,
            Gnys,P., Grote,P., Odermatt,B., Reutter,H.M. and Dworschak,G.C.
  TITLE     X-linked variations in SHROOM4 are implicated in congenital
            anomalies of the urinary tract and the anorectal, cardiovascular
            and central nervous systems
  JOURNAL   J Med Genet 60 (6), 587-596 (2023)
   PUBMED   36379543
  REMARK    GeneRIF: X-linked variations in SHROOM4 are implicated in
            congenital anomalies of the urinary tract and the anorectal,
            cardiovascular and central nervous systems.
REFERENCE   2  (bases 1 to 14557)
  AUTHORS   Zapata,J., Moretto,E., Hannan,S., Murru,L., Longatti,A., Mazza,D.,
            Benedetti,L., Fossati,M., Heise,C., Ponzoni,L., Valnegri,P.,
            Braida,D., Sala,M., Francolini,M., Hildebrand,J., Kalscheuer,V.,
            Fanelli,F., Sala,C., Bettler,B., Bassani,S., Smart,T.G. and
            Passafaro,M.
  TITLE     Epilepsy and intellectual disability linked protein Shrm4
            interaction with GABABRs shapes inhibitory neurotransmission
  JOURNAL   Nat Commun 8, 14536 (2017)
   PUBMED   28262662
  REMARK    GeneRIF: Shrm4 interaction with GABA-B receptors shapes inhibitory
            neurotransmission.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 14557)
  AUTHORS   Dietz,M.L., Bernaciak,T.M., Vendetti,F., Kielec,J.M. and
            Hildebrand,J.D.
  TITLE     Differential actin-dependent localization modulates the
            evolutionarily conserved activity of Shroom family proteins
  JOURNAL   J Biol Chem 281 (29), 20542-20554 (2006)
   PUBMED   16684770
REFERENCE   4  (bases 1 to 14557)
  AUTHORS   Hagens,O., Ballabio,A., Kalscheuer,V., Kraehenbuhl,J.P.,
            Schiaffino,M.V., Smith,P., Staub,O., Hildebrand,J. and
            Wallingford,J.B.
  TITLE     A new standard nomenclature for proteins related to Apx and Shroom
  JOURNAL   BMC Cell Biol 7, 18 (2006)
   PUBMED   16615870
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 14557)
  AUTHORS   Hagens,O., Dubos,A., Abidi,F., Barbi,G., Van Zutven,L.,
            Hoeltzenbein,M., Tommerup,N., Moraine,C., Fryns,J.P., Chelly,J.,
            van Bokhoven,H., Gecz,J., Dollfus,H., Ropers,H.H., Schwartz,C.E.,
            de Cassia Stocco Dos Santos,R., Kalscheuer,V. and Hanauer,A.
  TITLE     Disruptions of the novel KIAA1202 gene are associated with X-linked
            mental retardation
  JOURNAL   Hum Genet 118 (5), 578-590 (2006)
   PUBMED   16249884
REFERENCE   6  (bases 1 to 14557)
  AUTHORS   Stocco dos Santos,R.C., Castro,N.H., Lillia Holmes,A., Becak,W.,
            Tackels-Horne,D., Lindsey,C.J., Lubs,H.A., Stevenson,R.E. and
            Schwartz,C.E.
  TITLE     Stocco dos Santos X-linked mental retardation syndrome: clinical
            elucidation and localization to Xp11.3-Xq21.3
  JOURNAL   Am J Med Genet A 118A (3), 255-259 (2003)
   PUBMED   12673656
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL359272.9, AL121865.7 and
            AC233286.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Mar 29, 2021 this sequence version replaced NM_020717.4.
            
            Summary: This gene encodes a member of the APX/Shroom family, which
            contain an N-terminal PDZ domain and a C-terminal ASD2 motif. The
            encoded protein may play a role in cytoskeletal architecture.
            Mutations in this gene have been linked to the X-linked Stocco dos
            Santos syndrome characterized by cognitive disabilities.
            Alternatively spliced transcript variants have been described.
            [provided by RefSeq, Jul 2017].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the functional protein.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038193.3703926.1,
                                           SRR14372080.697318.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2158569, SAMN03267750
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000376020.9/ ENSP00000365188.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-293               AL359272.9         83951-84243         c
            294-445             AL121865.7         3161-3312
            446-580             AL121865.7         60790-60924
            581-3071            AL121865.7         63430-65920
            3072-3133           AL121865.7         71423-71484
            3134-3937           AC233286.2         1117-1920
            3938-4118           AC233286.2         6488-6668
            4119-4388           AC233286.2         10766-11035
            4389-14557          AC233286.2         12337-22505
FEATURES             Location/Qualifiers
     source          1..14557
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xp11.22"
     gene            1..14557
                     /gene="SHROOM4"
                     /gene_synonym="MRXSSDS; SHAP; shrm4"
                     /note="shroom family member 4"
                     /db_xref="GeneID:57477"
                     /db_xref="HGNC:HGNC:29215"
                     /db_xref="MIM:300579"
     exon            1..293
                     /gene="SHROOM4"
                     /gene_synonym="MRXSSDS; SHAP; shrm4"
                     /inference="alignment:Splign:2.1.0"
     CDS             177..4658
                     /gene="SHROOM4"
                     /gene_synonym="MRXSSDS; SHAP; shrm4"
                     /note="second homolog of apical protein"
                     /codon_start=1
                     /product="protein Shroom4"
                     /protein_id="NP_065768.2"
                     /db_xref="CCDS:CCDS35277.1"
                     /db_xref="GeneID:57477"
                     /db_xref="HGNC:HGNC:29215"
                     /db_xref="MIM:300579"
                     /translation="MENRPGSFQYVPVQLQGGAPWGFTLKGGLEHCEPLTVSKIEDGG
                     KAALSQKMRTGDELVNINGTPLYGSRQEALILIKGSFRILKLIVRRRNAPVSRPHSWH
                     VAKLLEGCPEAATTMHFPSEAFSLSWHSGCNTSDVCVQWCPLSRHCSTEKSSSIGSME
                     SLEQPGQATYESHLLPIDQNMYPNQRDSAYSSFSASSNASDCALSLRPEEPASTDCIM
                     QGPGPTKAPSGRPNVAETSGGSRRTNGGHLTPSSQMSSRPQEGYQSGPAKAVRGPPQP
                     PVRRDSLQASRAQLLNGEQRRASEPVVPLPQKEKLSLEPVLPARNPNRFCCLSGHDQV
                     TSEGHQNCEFSQPPESSQQGSEHLLMQASTKAVGSPKACDRASSVDSNPLNEASAELA
                     KASFGRPPHLIGPTGHRHSAPEQLLASHLQHVHLDTRGSKGMELPPVQDGHQWTLSPL
                     HSSHKGKKSPCPPTGGTHDQSSKERKTRQVDDRSLVLGHQSQSSPPHGEADGHPSEKG
                     FLDPNRTSRAASELANQQPSASGSLVQQATDCSSTTKAASGTEAGEEGDSEPKECSRM
                     GGRRSGGTRGRSIQNRRKSERFATNLRNEIQRRKAQLQKSKGPLSQLCDTKEPVEETQ
                     EPPESPPLTASNTSLLSSCKKPPSPRDKLFNKSMMLRARSSECLSQAPESHESRTGLE
                     GRISPGQRPGQSSLGLNTWWKAPDPSSSDPEKAHAHCGVRGGHWRWSPEHNSQPLVAA
                     AMEGPSNPGDNKELKASTAQAGEDAILLPFADRRKFFEESSKSLSTSHLPGLTTHSNK
                     TFTQRPKPIDQNFQPMSSSCRELRRHPMDQSYHSADQPYHATDQSYHSMSPLQSETPT
                     YSECFASKGLENSMCCKPLHCGDFDYHRTCSYSCSVQGALVHDPCIYCSGEICPALLK
                     RNMMPNCYNCRCHHHQCIRCSVCYHNPQHSALEDSSLAPGNTWKPRKLTVQEFPGDKW
                     NPITGNRKTSQSGREMAHSKTSFSWATPFHPCLENPALDLSSYRAISSLDLLGDFKHA
                     LKKSEETSVYEEGSSLASMPHPLRSRAFSESHISLAPQSTRAWGQHRRELFSKGDETQ
                     SDLLGARKKAFPPPRPPPPNWEKYRLFRAAQQQKQQQQQQKQQEEEEEEEEEEEEEEE
                     EEEEEAEEEEEELPPQYFSSETSGSCALNPEEVLEQPQPLSFGHLEGSRQGSQSVPAE
                     QESFALHSSDFLPPIRGHLGSQPEQAQPPCYYGIGGLWRTSGQEATESAKQEFQHFSP
                     PSGAPGIPTSYSAYYNISVAKAELLNKLKDQPEMAEIGLGEEEVDHELAQKKIQLIES
                     ISRKLSVLREAQRGLLEDINANSALGEEVEANLKAVCKSNEFEKYHLFVGDLDKVVNL
                     LLSLSGRLARVENALNSIDSEANQEKLVLIEKKQQLTGQLADAKELKEHVDRREKLVF
                     GMVSRYLPQDQLQDYQHFVKMKSALIIEQRELEEKIKLGEEQLKCLRESLLLGPSNF"
     exon            294..445
                     /gene="SHROOM4"
                     /gene_synonym="MRXSSDS; SHAP; shrm4"
                     /inference="alignment:Splign:2.1.0"
     exon            446..580
                     /gene="SHROOM4"
                     /gene_synonym="MRXSSDS; SHAP; shrm4"
                     /inference="alignment:Splign:2.1.0"
     exon            581..3071
                     /gene="SHROOM4"
                     /gene_synonym="MRXSSDS; SHAP; shrm4"
                     /inference="alignment:Splign:2.1.0"
     exon            3072..3133
                     /gene="SHROOM4"
                     /gene_synonym="MRXSSDS; SHAP; shrm4"
                     /inference="alignment:Splign:2.1.0"
     exon            3134..3937
                     /gene="SHROOM4"
                     /gene_synonym="MRXSSDS; SHAP; shrm4"
                     /inference="alignment:Splign:2.1.0"
     exon            3938..4118
                     /gene="SHROOM4"
                     /gene_synonym="MRXSSDS; SHAP; shrm4"
                     /inference="alignment:Splign:2.1.0"
     exon            4119..4388
                     /gene="SHROOM4"
                     /gene_synonym="MRXSSDS; SHAP; shrm4"
                     /inference="alignment:Splign:2.1.0"
     exon            4389..14557
                     /gene="SHROOM4"
                     /gene_synonym="MRXSSDS; SHAP; shrm4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      12391..12396
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SHROOM4"
                     /gene_synonym="MRXSSDS; SHAP; shrm4"
                     /note="hexamer: AATAAA"
     polyA_site      12414
                     /gene="SHROOM4"
                     /gene_synonym="MRXSSDS; SHAP; shrm4"
     regulatory      14538..14543
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SHROOM4"
                     /gene_synonym="MRXSSDS; SHAP; shrm4"
                     /note="hexamer: AATAAA"
     polyA_site      14557
                     /gene="SHROOM4"
                     /gene_synonym="MRXSSDS; SHAP; shrm4"
                     /note="major polyA site"
ORIGIN      
        1 gtcatttcct ccttgcgctc gctcgctgcc tgagcgtctc cagccgggag agtaggcgga
       61 gcagggcggt gcggggtggc gtagagctgg agggcgatgg tggcggagct gctgggggcg
      121 gaggcaacgt agccccctcg gaaaaggagc ccggcggcgc ctgagcccag ccgaggatgg
      181 agaaccggcc tgggtccttc cagtacgtcc ctgtgcagct gcaagggggg gcaccctggg
      241 gcttcaccct taaggggggt ctggaacact gtgagccgct cacagtgtct aagattgaag
      301 atggaggcaa ggcagctttg tcccagaaga tgaggactgg tgatgagctg gtgaatatca
      361 atggcactcc attatatggc tcccgccaag aggccctcat tctcatcaaa ggctccttcc
      421 ggattctcaa gctgattgtc aggaggagga acgcccctgt cagtaggccg cactcatggc
      481 atgtggccaa gctgctggag ggatgccctg aagcagccac caccatgcat ttcccttctg
      541 aagccttcag cttgtcctgg cattctggct gcaacacaag tgacgtgtgt gtgcagtggt
      601 gtccactctc ccggcattgc agcaccgaga aaagcagctc cattggcagc atggagagcc
      661 tggagcaacc aggccaagcc acctatgaga gccatctgtt gcctattgac cagaacatgt
      721 accctaacca gcgtgactca gcctacagct ccttctcggc cagctcaaat gcttctgact
      781 gtgccctttc cctcaggcca gaggagccag cctctacaga ctgcatcatg caaggcccag
      841 ggccaactaa ggcccccagt ggccggccta atgtggctga gacctcagga ggtagtcggc
      901 gcaccaatgg gggccacctg acccccagct ctcagatgtc atcccgtcca caggagggat
      961 accagtcagg gcccgccaaa gcagtcaggg gcccaccaca acctccagtg aggcgggaca
     1021 gccttcaggc ctccagagcc caactcctca atggagagca gcgcagggca tctgagcctg
     1081 tggtcccctt gccacagaag gagaaactga gcttagagcc tgtgctaccc gcaaggaacc
     1141 ctaataggtt ctgttgcctc agtgggcatg accaagtgac aagtgagggc catcagaact
     1201 gtgagttcag tcagcctcct gaatccagcc aacagggctc tgagcatcta ctgatgcagg
     1261 cctcaaccaa agctgttgga tccccaaaag cctgtgacag agcttccagc gtggattcca
     1321 acccactcaa tgaggcttct gcagagctag ctaaggcttc ttttggcaga cctccacatc
     1381 tcataggacc cacagggcat cgccatagtg cccctgaaca gctgctggca tcccacctgc
     1441 agcatgtgca ccttgatacc aggggcagca aagggatgga gctcccaccc gtacaggatg
     1501 ggcaccagtg gactctgtcc cctttgcaca gcagccacaa agggaagaaa agtccatgcc
     1561 cccctacagg aggaacccat gaccagtcca gcaaagaaag aaagaccaga caagtggatg
     1621 acaggtcttt agttttggga caccagagcc aaagcagtcc cccacatgga gaggctgatg
     1681 gacacccctc agaaaaaggt ttcctggacc caaacagaac aagcagagca gccagtgaat
     1741 tggccaacca gcaaccctct gcctctggct cccttgttca acaagccacg gactgttctt
     1801 caaccactaa agcagctagt ggcacagagg caggtgaaga aggggacagc gagcccaagg
     1861 agtgcagccg gatgggtggt aggcgaagtg gagggacccg gggccgctcg atccaaaacc
     1921 ggcggaagag tgagcgtttt gctaccaatc tgcgtaatga aattcagagg aggaaggccc
     1981 agctccagaa aagcaagggt cccttgtcac agctgtgtga cactaaggag ccagtggaag
     2041 agacccagga gcccccagaa agtcctccac tcactgcctc taacacatct cttctatctt
     2101 catgtaaaaa acctcccagc cccagagaca agctcttcaa caaaagcatg atgctcagag
     2161 ctaggtcttc cgagtgcctc agccaagccc ctgagagcca tgaatctagg acaggcttag
     2221 agggacgaat aagccctggc cagaggcctg gccagtcctc tttgggcctg aacacctggt
     2281 ggaaagcacc tgacccatcc tcctcagacc ctgagaaagc acatgctcac tgtggagtcc
     2341 gtggaggtca ttggagatgg tctccagagc ataattcaca gccacttgtg gcagcagcca
     2401 tggaaggccc ttccaaccca ggtgacaaca aggaattgaa ggcttctact gctcaagctg
     2461 gggaggatgc catcctcttg ccttttgcag acagaagaaa gttctttgaa gagagtagca
     2521 aatccttatc tacatctcat ttgccaggtt taaccactca tagcaacaag acttttaccc
     2581 agagaccaaa acctatagac caaaacttcc agccaatgag ctccagctgt agggaattga
     2641 ggcgccatcc catggaccaa tcatatcatt ccgcagacca accatatcat gccacagacc
     2701 aatcatatca ttccatgtca ccccttcagt cagaaactcc cacttactca gaatgttttg
     2761 caagcaaagg tctagaaaat tccatgtgtt gtaagccact acactgtggt gattttgatt
     2821 accacaggac ctgctcttac tcctgcagtg ttcaaggagc tctagtccat gatccttgca
     2881 tttattgttc tggggaaatc tgccctgcct tgctaaagag aaatatgatg ccaaattgct
     2941 acaactgccg gtgccaccac caccaatgca ttcggtgttc agtttgctat cataatcctc
     3001 agcacagtgc cctcgaggac agcagcttgg cacctggcaa cacttggaaa cccaggaagc
     3061 tgacagtgca ggaatttcct ggggacaaat ggaatccaat aacaggaaac aggaagacca
     3121 gccagtcagg gagggaaatg gctcattcca agactagctt ttcatgggca acccctttcc
     3181 atccttgcct tgagaaccca gcactggact tgtcaagcta ccgagcaatt tcttctcttg
     3241 acctccttgg agacttcaaa catgctttga aaaaatcaga ggaaacttca gtttatgagg
     3301 aggggagctc ccttgcctcc atgccccacc cactgcgcag ccgtgccttc tcagagagtc
     3361 acatcagctt ggcgccccaa agcacccggg cctgggggca gcataggagg gagctcttta
     3421 gcaaaggtga tgagacccag tcggatcttc tcggagccag gaagaaggcc tttcctcctc
     3481 ctcgccctcc tcctcccaac tgggagaagt acaggctctt tcgtgcagcc cagcagcaga
     3541 agcagcaaca gcagcagcag aagcaacagg aggaggagga ggaggaggaa gaagaagaag
     3601 aagaggaaga ggaagaggag gaggaggagg cagaggagga ggaagaggag ctgccacccc
     3661 agtatttcag ttcagaaacc tctggttcct gtgctctcaa tcctgaggag gtcctagagc
     3721 agccacaacc cctcagcttt ggccacctgg agggctcgag acagggttca caaagtgtcc
     3781 cagcagagca agaatccttt gcactccatt ccagtgattt cttgcctcca ataaggggtc
     3841 acttgggatc tcaacctgag caggctcagc ccccttgcta ctatggcatt ggtgggcttt
     3901 ggaggacatc gggacaggaa gccactgaat ccgccaaaca agagtttcag cacttttcgc
     3961 ctccttcagg ggccccagga atccctacct cttactcagc ttattacaat atttctgtgg
     4021 ccaaggcaga gctgctgaac aaactgaaag accaacctga gatggcagag attggcctag
     4081 gagaggagga agttgaccat gaactggctc aaaaaaagat acagcttatc gaaagcatca
     4141 gcagaaaact ttctgtcttg cgggaggccc agcgagggct gctagaggac atcaatgcca
     4201 attctgccct tggggaggag gtggaggcca acttaaaagc cgtctgcaaa tccaatgaat
     4261 ttgaaaagta ccacttgttt gttggggacc tggacaaagt ggtcaacctg ttgctgtcac
     4321 tctctggacg actggcccgg gtggagaatg ctctgaacag catcgattca gaggccaacc
     4381 aggagaagtt ggtactgata gagaagaagc agcagctgac ggggcagttg gcagatgcca
     4441 aggagctgaa ggagcacgtg gaccgccggg agaagttggt gtttggcatg gtctcccgct
     4501 acctgcctca ggaccagctc caagattacc agcactttgt caagatgaaa tctgctctca
     4561 tcattgaaca gcgagagctg gaggagaaga tcaagctcgg ggaagagcaa ctcaaatgtc
     4621 tcagggagag tctactcctg gggcccagca atttctaatt ctaccagcac tctgccacag
     4681 catccctgcc cagccatgtg ggaagtgctt tcaatcttct ttgttagcag tttctcagca
     4741 agtagatagc aattagcagt ttgttccagc cctctaccct ggatgtctct cactacccct
     4801 tccctagcag tggtcctaac cagctaggag accctgggga agccacaagc ttctacccaa
     4861 gggagctgca gcaaggtgtg atcttagaac cacactctcc ttcccacagt tgccaagggc
     4921 aagtacttgc tgcacagaga accaaggaag tgccttcatt ctgctttgta ctaggacacc
     4981 aaagacatca agtactcatc acccacccat atcatcaaca gcctctaaag gctcagaggg
     5041 aatctgcctt gcagctctac tctgccccag ggcttgtggc cagccatttc tcacagagag
     5101 ctggctgcct tgagggcatt cacctggcac cagtttcagg gcctcaccca agctttgcag
     5161 gggaaagcac agagggagga attacactga aaaaaatgca agcaaaggtt gagtaccccc
     5221 aggtgcccct taggaaggaa ccaggtttaa ataggctcta cccttacctt tcccagcagc
     5281 aagttcaggg gaagaggcct actcttagcc ctggctagtg tgaccctctt cctgtcctaa
     5341 gactttggtc ctaccacctc ttgtttcatc tttcctttac attgctgggg gttaccgcag
     5401 gtgcctaccc cagggcttca ccatatgggc cattaatagc tctactaaaa ctgacttcta
     5461 gatgtaggtt tcattattgg gggagggggt tcttattgtt atattttaaa tggccttttg
     5521 attttattta tttttatgtt ttgattattt ttttcttttt taactaataa ggcgagaaga
     5581 gggaagttgg agagggaaaa gttagcccag aaggaaagca ttttctgcag atcagcctga
     5641 atccaccgtg gctaggtaag caagtgccaa ggcgttgaat tgttcccacc atagctaccg
     5701 atgtctagga aggagttttc cccttgaact cagagcctcc ctactgtggc ctggccttgt
     5761 gcttccctta aagtctaagt gacttgagtt tagcattccc cagcagggtt taagtttttc
     5821 ttactgattt ctttttgaaa ccaaagaaat ctcctgaaat ggagaaaggt tcagtcattt
     5881 ctctaccatc tccaagctat gggatcagta gaggtgagct cagaaggaac tcctctaacc
     5941 attgctttct cagcctgtgg cctggctcaa gggaaccaaa actcaaggta gctgggagct
     6001 ccagccagag ccagctcccc agagcaattg aagacccagc caggatctta ggcatacaaa
     6061 caactaaaac tgctgctgtt gccaaggccc tgacaccagt agctagtttc tatgctggaa
     6121 aagcagccct caggtggacg atggcaagaa gggggttatg gccaaatgct gccctgttat
     6181 ccagcttagc tccctgttcc tcactaactg gggccaatcc ttggtatcac acaacccagc
     6241 caaggcctgg catctcttct agttactgct gagttcctca gggtccttga aggtcaactg
     6301 tagcagcagg gatgtctcat tcaaccaaag aatttgccaa ggaacttttg ctgctgttac
     6361 aaatacttgt cactagcttc catttcctct tcattgtatt tgaaaacaat gggagaatct
     6421 tggccagcag ggtgtcctgg ggtagggggt ggggtaagcc tttctgtgga gctctgggtt
     6481 tgctcttagt actgctgcca gaaacactgt tagaatggca accctgccat tctcccctcc
     6541 agtggaagat tctactttat tcaggaaggc tggtggcttg caggggctag gcttacacaa
     6601 gccagccaag attgagaagg taggggagtg ctttcaatgg aggccatagt tgtcatcttt
     6661 tcttctgagc aggctctagg acaaacaatg agcaaattga gatgtcctgt attgggagga
     6721 tgaggctgat atgtttcatc attctcagaa tttgtgggct agagcacctt tttgggaata
     6781 tgggcctagg gaaaagaacg gtcacttacc tggacactat ggccatagtc ttgagttttt
     6841 ctagtcagca tgtgattggt tgtaccttaa gtttatgaca ccaaaaatac tattaactct
     6901 gttatttttg ttcttaatcc catctcaaat tagtttctag agttagaatc agaacacaag
     6961 gatgctcatt ttcacatgga gggaaagttg ccaaagcatt taaaaaataa ccccaaaagt
     7021 atatctagca gtgaagcttg gtagataaag gtgaactgct aaacagacac tggcgtgcca
     7081 cactcctgcc agggcagtct cattgtgtgt gacggtgctg caacactgcc agtattactt
     7141 gagatgcagt ctctctcccc tgttctcagt tcctgggctc agccctcctc caaggcctgc
     7201 cagtgagtag cagtttggaa ggcaggccaa gggagatccc caaagacagt tatcagtctt
     7261 aacttctgct gtctcccgtc aaatacttat acaggccccc atgggtaata ggcaagcatg
     7321 atggctgata gagaagtcag tgcatgagtt actatcacat gtccccccaa cccctcctgc
     7381 cacctcccag ggctctggat aattgaatct tcctgagtcc cacagctgga gactcaacca
     7441 ggatatagct gtaaatgccc aagtagaatc tgacagaata agacagagac actgaaaata
     7501 aagccctaga aaggaagaaa ttggaagcaa agaaaaggag aggtgaaaag ataaaaagcc
     7561 tccttcaagg ttaggttcag gttctgtttt ccatttaacc tcatgtgcca taaagctgcc
     7621 caggcacacc agagccacat cctgaacccg accctccctg acagtgctgc tctgccagta
     7681 gcaagcccca gatggaggaa gctgggccca tttctggcca cttccaccca tttggagctt
     7741 tgccagagga gtcgtctatg ccaataatat ttctgcaaca gcatattata ttatttgaag
     7801 attagtagat ctttttgggg gggtggggca ggggacagtt tctatagatg aagaaccagt
     7861 gttggttgta cagctgttgg gggtcatcta tcccatgtga agctattctt tttccaaatc
     7921 ttgttgtttc tgcatttgtg tcctccacca ctcccttctt ggctgacata gatatgcctg
     7981 ccagattgtc atcaagggtc atatttcaat aaaaggtgct aaggacaaaa aaaaatctca
     8041 tgtgttttaa ctcaggtgat ggaagtctaa agattctgct gaaaagtact ggaaaggaat
     8101 ttggtcacct atccatagtc cttcacagat aaacatatga ccagaccctt acccacatcc
     8161 ctaaaccctt accctggcct ctgagggatg gtattgatgc tataccattc atcatcccca
     8221 gttaaatagc aggatctgcc atagcctttt taggggaaca gcttttagac atctatttca
     8281 agaccatcct gagtaggggg tacagagttt aagccattgg ttatttctgt tgttatcaca
     8341 gtattgaagc atggtatttt tctactcaga aggatctcag gatcacagcc ctgcccttta
     8401 gctcactgat atatttcttc ctctctggtt actcagtggc aacatcacat ccagtctaac
     8461 cccatgttta gaatgcagaa aaagtcaaca ataggtgtaa tctcaggtat agacctaagc
     8521 agtatacaga gtctagttct ggagtctagc ctgatcgaag gtaagccatt ctttctagac
     8581 ttcttccaat cttgaattct gtctagtatt ttcatcttac ttctagctga tagtccaatc
     8641 tcacaccttc atgctcagct ccctggaaag caagggcctt ggtgatctgc tctccaaggc
     8701 aacctcaaag attagactca aatgtattcc aggaagcaac tctgacattg aaaagctttc
     8761 ttttttcgtg atccatattc tgttcacatt ccaactatcc tccttctact caagatatcc
     8821 attgtggtag agtagaaaga gtccagcttt ggcattagaa tgaactgtgt tcaaatcctg
     8881 gttctgccac ttattcaatg aacctgggct actagctcaa cttctgaggt cattttctta
     8941 tctgtaaaat gggaacagta ttatcttgct tgcagttatt gtaacttgaa aataataaat
     9001 aaaagccact aacaatccaa gaagttatta ggcagaatcc caggcccacc ccaaacccag
     9061 tgatagcagt ctgaatttta actagatttc caggagattc atgaacatat tacagcatgg
     9121 gaagtactga cttgttgaaa acatattcag tacatggtaa ccattattat agtcctgatc
     9181 tactcaattc attttttcct tatcccaggc atattcttgc tcttctcgtg ttgctcacaa
     9241 ctacctgcct ggatgaattt aggaaagttg caggatacaa ggttaaaaca caagatcaaa
     9301 tgaacaatcc gaaaatgtta ttaagaaaac agttccggcc gggcatggtg gctcacgcct
     9361 gaaatcccag cactttggga ggccgaggca ggtggatcac gaggtcagga gatcaagacc
     9421 atcctggcta acacggtgaa accctatctc tactaaaaat acaaaaaatt agccaggtgt
     9481 ggtggcacgc accagtagtc ccagctactc gggaggctga ggcaggagaa ttgcttgaac
     9541 ctggaaggca gagattgcag tgagctgaga ccacaccact gcactccatc ctgggcaaca
     9601 gagtgagact ttgtctcaaa aagaaagaaa gaaagaaaga aagaaagaaa gaaagaaaag
     9661 aaagaaagaa agaaagaaag aaaacagttc catttacaat agcatcaaaa agaaaaaagt
     9721 acttaggaat aatttaacaa aagaagtgcg aaacttttac actgaaaact ataaaacatt
     9781 gttgaaagag attagagaag atcaaaataa atggaaagac actccatgtt catggattgg
     9841 aagacttagt attgttaaga tgaaatgtgc cccaaattga tctacagatt caatccagtg
     9901 cttatcagaa ttccagtggc ttccttgtgt aagtttacaa gctgattcta aaattcatat
     9961 ggaattacaa gggacctgga acagtcaaaa caatctttaa aaagaacagt tggagggctc
    10021 atacatccca acttcaaaac ttactgcaaa actacagtaa tcaagacagt gtggtacaga
    10081 cacaagggta ggtgtataaa taaatggaat taaattgaga gtccagaaat aaatcctcat
    10141 gtatatggtt agttaatttc aacaagggtc ccaagagaaa ttgaggagag aatagttttt
    10201 taacaaatga tgctgagaca actgggtatc tacatgggaa aaaatgaagt tttccttaaa
    10261 tcacatgcaa gaatcaactc aaaatggatt atagaatgta agagtcaaaa ttataaaatt
    10321 cttagaagaa aacattgtaa taagtcttcc taatgttaag acgatttgca caagtgatac
    10381 aagaaataga aataaattgg agtaaatcaa aattaaaaac ttttgtgcta aaaatatacc
    10441 atataaaaaa gtgaaaagac aagcaacata atattaggag aaaatatttg catatcagat
    10501 acttcaaaat atgaaaagga ctcttacaac tcaatgataa aaataaccaa ttaaaaatgg
    10561 gttaacagat tgtaatagac atttcttcaa agaaaatatg tgttttagta tgcttgggtt
    10621 gctataacat actaccatag actgattgtc ttaaataaca tttatttctc acagtcctgg
    10681 agactgggaa atctgagagc aagatgccag cagattcaat gccttgtgag ggcccacttt
    10741 atggtttgca gatggctacc ttgtggctgt gtcctcagca aagacagaaa gggatctggt
    10801 ctcggccagg cgcggtggct cacgcctgta atcccagcac tttgggaggc cgtggcgggc
    10861 ggatcaggag gtcaggagat cgcaaccatc ctggctaaca cggtgaaacc ccatctctac
    10921 taaaaaaaat acaaaaaaat tagcagggca tggtggcagg tgcctgtagt cccagctagt
    10981 cgggaggctg aggcaggaga atggcgtgaa cccaggaggc ggagcttgca gtgagcagag
    11041 attgagccac tgcactccag cctgggtgac tgagcgagat tctgtctcaa aaaaaagaaa
    11101 gggatctggt ctcttcccct tcttataagg gcattataat ttcattatga atgcctactc
    11161 ttgtgacttc atctaaacca atttacctct caaaggcccc acctccatat gccatcacat
    11221 tggggattag ggcttcagca tgtgagtttg gagaggacac aaacattcag cttatagcaa
    11281 tatgcaactg gctagcaagc acagggaaag atgctcaagc actactattc attagggaaa
    11341 tgcaaatcaa gaccacaatg agataccact tcatacccac tagaatagct atatcaaaaa
    11401 gccaaacaat aacaagtgtt ggtgaggaaa tgaagaaatt ggaacactca tacattgctg
    11461 atggggatgt aaattgatgc aacctcattg aaaaacagct tggcagtttc tccaaatgtt
    11521 aaacatagaa tttccacatg acttagcaat tccatcccta ggtatatacc caagagaatt
    11581 ggaaacaggt gttcaaacaa aagcgtgaac atgaatgttt aaagtggcat tactcataat
    11641 agccaaaaag tggaaatgac ccaaatgtcc atccactgag gaatggataa acaaactttg
    11701 atatatgcat acagtagggt attatttagc cataaaaatg gataaaatac tgatacatgt
    11761 aacattgatg aaccttgaaa atatgctaag tgaaaggagc caatcacaaa agaccacata
    11821 ttgtatgatt taatttatat gaaatgtccc aaacaggcaa atctataaag gtagaaagca
    11881 gattagtggt tgtcaaggct gggggctcag gggcaatagg catggggttt cttttgggga
    11941 tgatgaaaat attataaaat tagattgtca tcctggttgt ccaactctga atgtactgga
    12001 aaccattgaa gtatacattt taaatgggtg tgctaaatgg tatatgaatt atttctcaat
    12061 aagtgtgttt ttttaaaaaa gctattgatt atttccatca gtctcattcc tcttgacaaa
    12121 aatctgagtt gatggtgagc atgtacttca ttcctgactc caaagggtac aatatttaca
    12181 atatttgaat ttgggagtga ctttactatt acaaccttcc ccttgaagac ctggaagacc
    12241 cagcaacata aggacaaaca gtagtctcag ccttgtacta agacttcagc aaaaataaga
    12301 gagatgggag ggactactat tgggaaggag gggcaatatt tctaggcaaa tattgtggta
    12361 gaatggataa gagcttcggc tcattgggca aataaaccaa gttttaaatc ctgattctac
    12421 caatttactg tgtcacctta ataagttact tgactcctct caaccttaat accttcatct
    12481 acagaagggg gctaataaaa gccccacctc aaagtgttac tatgttgatt aaatgagata
    12541 attcacatga agcacttaaa ataatgtctg ccatgtagta agtatttgat aaatgttagc
    12601 tgctagattt atttttattg aggtgagatt gaagtagaac aatataagga aaagtaaagc
    12661 atcttcagct ttggagtcag acagctctgt taatgtgacc ttggaagtca ctttatttat
    12721 tgagccttag caaataatga ggatagtaat ccttctctta cagaggcagt tccaagcctg
    12781 gacactggaa cccacaccac gtaggttttc atactggctc caccacttac acattgtgta
    12841 accttgagca tgtttaactt ccctgtacct ggatgtcctc ttctgtcaaa tggtggaaac
    12901 aaaaatacat ccttgtgttg tgaggaatta ttgagttaat ccatgcaaag tacttaggac
    12961 aataagtacc caatgaatgt taacttttat tataaagcta aagtaggaat caaatgaggt
    13021 ctctggtaga gctgttcata tggtaggttc tccgtagatg atggttcctt tccttaccta
    13081 ccctgctgtg tgcaccaggt caagattttt tggacctgca gataaaatgt ttcagcctta
    13141 ggagttcccc actgtaagta aaattttctg gcaagctcaa tactaccatc tgatgagaac
    13201 aatgtagtct gttagctgcc tgcaagatga ctagtctata ctcccaagaa aacaccccct
    13261 tcagaacaat agagaaggaa ctgttttgca tgtggaccac ttaaggtcca atttgaggct
    13321 ggtttgggga aaacatccac tcctcctcaa acctatcttt agacttaaag tcaacttaaa
    13381 tgtcattgag tttcccaccc tgggttaagc actttcactt atctttgctt acactatttt
    13441 tctatctaaa catacatttt taaagcagaa atcctcattt aatctttaaa acagatctgt
    13501 gattttgaaa cactatttaa tcaaaattca gaattgtggt agcatttttg ctgtttgtaa
    13561 gttaaacaaa atagattgta agtgtgcaat gccattcatt cattcaacta atgtgccaat
    13621 aatttgtttt gtgcttcagg gattcagaag tggacaagat agacaaagtc cccactctcg
    13681 tggggctttt atcctaatga aagagataga taagtataaa tatacaatta cataatactt
    13741 tatatacaga aatatacaca caatcacttt ttaccctggt atggcaatgc gaaacattct
    13801 tccttgattt cttcagctat gtgcaatagg ctaaactcat gaatctcttt gctactgcag
    13861 taactaaaat attttttgca ttaattttca ccagagaata atgtttcagt gaacatgaga
    13921 gtgcagatat ctcttcgaga tactgatttc atttcctttg ggtatttacc cagaagaaga
    13981 atctctgcat catatggtca tcgtattctt aatattttga ggaatataca ctattttcca
    14041 aaatggggta ctaatttaca ttcccaccaa caatgtacca gggtttcctt tcctccatac
    14101 cctcatcaac atttgttatc ccttgccttt ttgataaaag cctgctattt atgacaaaat
    14161 ggatgaaccc ggagggtatt atgctaagtg aaacaaacca gacacagaaa gaaaaatact
    14221 gcgtaatctc atatgtggaa gctaaaaaag tcaaacacac agggggatgg aggtggaaga
    14281 aatgaggatg ttggtcaaag ctacaaagtt gcagctacat aagatgtata aatctagaga
    14341 tctcatgtac agcataagtt ctatagttaa taatgctgta ttgaatactg gaaattttct
    14401 aagagtagat ttcaggtgct cttactgcaa aagagggtaa ttatgtgagg aaatgcatat
    14461 ttaatttgca tgactgtagc aatcatttca ctatgtaatg gatgtcaaaa catcatttgt
    14521 acaccttaaa tatatacaat aaaagatttt caccaaa
//
