LOCUS       NM_020859              10891 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens shroom family member 3 (SHROOM3), mRNA.
ACCESSION   NM_020859 XR_109824 XR_112647
VERSION     NM_020859.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10891)
  AUTHORS   Deshwar,A.R., Martin,N., Shannon,P. and Chitayat,D.
  TITLE     A homozygous pathogenic variant in SHROOM3 associated with
            anencephaly and cleft lip and palate
  JOURNAL   Clin Genet 98 (3), 299-302 (2020)
   PUBMED   32621286
  REMARK    GeneRIF: A homozygous pathogenic variant in SHROOM3 associated with
            anencephaly and cleft lip and palate.
REFERENCE   2  (bases 1 to 10891)
  AUTHORS   Corredor,Z., da Silva Filho,M.I., Rodriguez-Ribera,L., Catalano,C.,
            Hemminki,K., Coll,E., Silva,I., Diaz,J.M., Ballarin,J.A.,
            Henandez,A., Forsti,A., Marcos,R. and Pastor,S.
  TITLE     Loci associated with genomic damage levels in chronic kidney
            disease patients and controls
  JOURNAL   Mutat Res Genet Toxicol Environ Mutagen 852, 503167 (2020)
   PUBMED   32265040
  REMARK    GeneRIF: The three genes associated with chronic kidney disease
            (AGT, GLO1, and SHROOM3) showed associations with both the high
            levels of oxidatively damaged DNA and genomic instability.
REFERENCE   3  (bases 1 to 10891)
  AUTHORS   Wei,C., Banu,K., Garzon,F., Basgen,J.M., Philippe,N., Yi,Z.,
            Liu,R., Choudhuri,J., Fribourg,M., Liu,T., Cumpelik,A., Wong,J.,
            Khan,M., Das,B., Keung,K., Salem,F., Campbell,K.N., Kaufman,L.,
            Cravedi,P., Zhang,W., O'Connell,P.J., He,J.C., Murphy,B. and
            Menon,M.C.
  TITLE     SHROOM3-FYN Interaction Regulates Nephrin Phosphorylation and
            Affects Albuminuria in Allografts
  JOURNAL   J Am Soc Nephrol 29 (11), 2641-2657 (2018)
   PUBMED   30341149
  REMARK    GeneRIF: We confirmed the interaction of endogenous SHROOM3 with
            FYN in podocytes via a critical Src homology 3-binding domain,
            distinct from its ROCK-binding domain. Shroom3-Fyn interaction was
            required in vitro and in vivo for activation of Fyn kinase and
            downstream nephrin phosphorylation in podocytes.
REFERENCE   4  (bases 1 to 10891)
  AUTHORS   Prokop,J.W., Yeo,N.C., Ottmann,C., Chhetri,S.B., Florus,K.L.,
            Ross,E.J., Sosonkina,N., Link,B.A., Freedman,B.I., Coppola,C.J.,
            McDermott-Roe,C., Leysen,S., Milroy,L.G., Meijer,F.A., Geurts,A.M.,
            Rauscher,F.J. 3rd, Ramaker,R., Flister,M.J., Jacob,H.J.,
            Mendenhall,E.M. and Lazar,J.
  TITLE     Characterization of Coding/Noncoding Variants for SHROOM3 in
            Patients with CKD
  JOURNAL   J Am Soc Nephrol 29 (5), 1525-1535 (2018)
   PUBMED   29476007
  REMARK    GeneRIF: We determined that coding variant P1244L attenuates the
            interaction of SHROOM3 with 14-3-3, suggesting alterations to the
            Hippo pathway, a known mediator of CKD.
REFERENCE   5  (bases 1 to 10891)
  AUTHORS   Chen,Z., Kuang,L., Finnell,R.H. and Wang,H.
  TITLE     Genetic and functional analysis of SHROOM1-4 in a Chinese neural
            tube defect cohort
  JOURNAL   Hum Genet 137 (3), 195-202 (2018)
   PUBMED   29423651
  REMARK    GeneRIF: Functional variants were further confirmed by western blot
            and the mammalian two-hybrid assays. Loss of function (LoF)
            variants were identified in SHROOM3. We observed 1.56 times as many
            rare [minor allele frequency (MAF) < 0.01] coding variants (p = 2.9
            x 10(-3)) in SHROOM genes, and 4.5 times as many rare D-Mis
            (deleterious missense) variants in SHROOM2 genes in the Neural tube
            defects cases compared with the controls
REFERENCE   6  (bases 1 to 10891)
  AUTHORS   Rothenberg,M.E., Spergel,J.M., Sherrill,J.D., Annaiah,K.,
            Martin,L.J., Cianferoni,A., Gober,L., Kim,C., Glessner,J.,
            Frackelton,E., Thomas,K., Blanchard,C., Liacouras,C., Verma,R.,
            Aceves,S., Collins,M.H., Brown-Whitehorn,T., Putnam,P.E.,
            Franciosi,J.P., Chiavacci,R.M., Grant,S.F., Abonia,J.P.,
            Sleiman,P.M. and Hakonarson,H.
  TITLE     Common variants at 5q22 associate with pediatric eosinophilic
            esophagitis
  JOURNAL   Nat Genet 42 (4), 289-291 (2010)
   PUBMED   20208534
REFERENCE   7  (bases 1 to 10891)
  AUTHORS   Kottgen,A., Glazer,N.L., Dehghan,A., Hwang,S.J., Katz,R., Li,M.,
            Yang,Q., Gudnason,V., Launer,L.J., Harris,T.B., Smith,A.V.,
            Arking,D.E., Astor,B.C., Boerwinkle,E., Ehret,G.B., Ruczinski,I.,
            Scharpf,R.B., Chen,Y.D., de Boer,I.H., Haritunians,T., Lumley,T.,
            Sarnak,M., Siscovick,D., Benjamin,E.J., Levy,D., Upadhyay,A.,
            Aulchenko,Y.S., Hofman,A., Rivadeneira,F., Uitterlinden,A.G., van
            Duijn,C.M., Chasman,D.I., Pare,G., Ridker,P.M., Kao,W.H.,
            Witteman,J.C., Coresh,J., Shlipak,M.G. and Fox,C.S.
  TITLE     Multiple loci associated with indices of renal function and chronic
            kidney disease
  JOURNAL   Nat Genet 41 (6), 712-717 (2009)
   PUBMED   19430482
  REMARK    GeneRIF: Observational study, meta-analysis, and genome-wide
            association study of gene-disease association. (HuGE Navigator)
REFERENCE   8  (bases 1 to 10891)
  AUTHORS   Hagens,O., Ballabio,A., Kalscheuer,V., Kraehenbuhl,J.P.,
            Schiaffino,M.V., Smith,P., Staub,O., Hildebrand,J. and
            Wallingford,J.B.
  TITLE     A new standard nomenclature for proteins related to Apx and Shroom
  JOURNAL   BMC Cell Biol 7, 18 (2006)
   PUBMED   16615870
  REMARK    Publication Status: Online-Only
REFERENCE   9  (bases 1 to 10891)
  AUTHORS   Navarro-Lerida,I., Martinez Moreno,M., Roncal,F., Gavilanes,F.,
            Albar,J.P. and Rodriguez-Crespo,I.
  TITLE     Proteomic identification of brain proteins that interact with
            dynein light chain LC8
  JOURNAL   Proteomics 4 (2), 339-346 (2004)
   PUBMED   14760703
REFERENCE   10 (bases 1 to 10891)
  AUTHORS   Hildebrand,J.D. and Soriano,P.
  TITLE     Shroom, a PDZ domain-containing actin-binding protein, is required
            for neural tube morphogenesis in mice
  JOURNAL   Cell 99 (5), 485-497 (1999)
   PUBMED   10589677
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AB055660.1, AK127929.1,
            AC107072.3, AK128677.1 and AW025141.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_020859.3.
            
            Summary: This gene encodes a PDZ-domain-containing protein that
            belongs to a family of Shroom-related proteins. This protein may be
            involved in regulating cell shape in certain tissues. A similar
            protein in mice is required for proper neurulation. [provided by
            RefSeq, Jan 2011].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB055660.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2146982, SAMEA2163459
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000296043.7/ ENSP00000296043.6
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1280              AB055660.1         131-1410
            1281-1283           AK127929.1         412-414
            1284-1659           AB055660.1         1414-1789
            1660-2229           AC107072.3         41591-42160
            2230-4692           AB055660.1         2360-4822
            4693-7437           AK128677.1         3324-6068
            7438-10371          AC107072.3         82382-85315
            10372-10891         AW025141.1         4-523               c
FEATURES             Location/Qualifiers
     source          1..10891
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q21.1"
     gene            1..10891
                     /gene="SHROOM3"
                     /gene_synonym="APXL3; MSTP013; SHRM; ShrmL"
                     /note="shroom family member 3"
                     /db_xref="GeneID:57619"
                     /db_xref="HGNC:HGNC:30422"
                     /db_xref="MIM:604570"
     exon            1..992
                     /gene="SHROOM3"
                     /gene_synonym="APXL3; MSTP013; SHRM; ShrmL"
                     /inference="alignment:Splign:2.1.0"
     CDS             825..6815
                     /gene="SHROOM3"
                     /gene_synonym="APXL3; MSTP013; SHRM; ShrmL"
                     /note="F-actin-binding protein; shroom-related protein"
                     /codon_start=1
                     /product="protein Shroom3"
                     /protein_id="NP_065910.3"
                     /db_xref="CCDS:CCDS3579.2"
                     /db_xref="GeneID:57619"
                     /db_xref="HGNC:HGNC:30422"
                     /db_xref="MIM:604570"
                     /translation="MMRTTEDFHKPSATLNSNTATKGRYIYLEAFLEGGAPWGFTLKG
                     GLEHGEPLIISKVEEGGKADTLSSKLQAGDEVVHINEVTLSSSRKEAVSLVKGSYKTL
                     RLVVRRDVCTDPGHADTGASNFVSPEHLTSGPQHRKAAWSGGVKLRLKHRRSEPAGRP
                     HSWHTTKSGEKQPDASMMQISQGMIGPPWHQSYHSSSSTSDLSNYDHAYLRRSPDQCS
                     SQGSMESLEPSGAYPPCHLSPAKSTGSIDQLSHFHNKRDSAYSSFSTSSSILEYPHPG
                     ISGRERSGSMDNTSARGGLLEGMRQADIRYVKTVYDTRRGVSAEYEVNSSALLLQGRE
                     ARASANGQGYDKWSNIPRGKGVPPPSWSQQCPSSLETATDNLPPKVGAPLPPARSDSY
                     AAFRHRERPSSWSSLDQKRLCRPQANSLGSLKSPFIEEQLHTVLEKSPENSPPVKPKH
                     NYTQKAQPGQPLLPTSIYPVPSLEPHFAQVPQPSVSSNGMLYPALAKESGYIAPQGAC
                     NKMATIDENGNQNGSGRPGFAFCQPLEHDLLSPVEKKPEATAKYVPSKVHFCSVPENE
                     EDASLKRHLTPPQGNSPHSNERKSTHSNKPSSHPHSLKCPQAQAWQAGEDKRSSRLSE
                     PWEGDFQEDHNANLWRRLEREGLGQSLSGNFGKTKSAFSSLQNIPESLRRHSSLELGR
                     GTQEGYPGGRPTCAVNTKAEDPGRKAAPDLGSHLDRQVSYPRPEGRTGASASFNSTDP
                     SPEEPPAPSHPHTSSLGRRGPGPGSASALQGFQYGKPHCSVLEKVSKFEQREQGSQRP
                     SVGGSGFGHNYRPHRTVSTSSTSGNDFEETKAHIRFSESAEPLGNGEQHFKNGELKLE
                     EASRQPCGQQLSGGASDSGRGPQRPDARLLRSQSTFQLSSEPEREPEWRDRPGSPESP
                     LLDAPFSRAYRNSIKDAQSRVLGATSFRRRDLELGAPVASRSWRPRPSSAHVGLRSPE
                     ASASASPHTPRERHSVTPAEGDLARPVPPAARRGARRRLTPEQKKRSYSEPEKMNEVG
                     IVEEAEPAPLGPQRNGMRFPESSVADRRRLFERDGKACSTLSLSGPELKQFQQSALAD
                     YIQRKTGKRPTSAAGCSLQEPGPLRERAQSAYLQPGPAALEGSGLASASSLSSLREPS
                     LQPRREATLLPATVAETQQAPRDRSSSFAGGRRLGERRRGDLLSGANGGTRGTQRGDE
                     TPREPSSWGARAGKSMSAEDLLERSDVLAGPVHVRSRSSPATADKRQDVLLGQDSGFG
                     LVKDPCYLAGPGSRSLSCSERGQEEMLPLFHHLTPRWGGSGCKAIGDSSVPSECPGTL
                     DHQRQASRTPCPRPPLAGTQGLVTDTRAAPLTPIGTPLPSAIPSGYCSQDGQTGRQPL
                     PPYTPAMMHRSNGHTLTQPPGPRGCEGDGPEHGVEEGTRKRVSLPQWPPPSRAKWAHA
                     AREDSLPEESSAPDFANLKHYQKQQSLPSLCSTSDPDTPLGAPSTPGRISLRISESVL
                     RDSPPPHEDYEDEVFVRDPHPKATSSPTFEPLPPPPPPPPSQETPVYSMDDFPPPPPH
                     TVCEAQLDSEDPEGPRPSFNKLSKVTIARERHMPGAAHVVGSQTLASRLQTSIKGSEA
                     ESTPPSFMSVHAQLAGSLGGQPAPIQTQSLSHDPVSGTQGLEKKVSPDPQKSSEDIRT
                     EALAKEIVHQDKSLADILDPDSRLKTTMDLMEGLFPRDVNLLKENSVKRKAIQRTVSS
                     SGCEGKRNEDKEAVSMLVNCPAYYSVSAPKAELLNKIKEMPAEVNEEEEQADVNEKKA
                     ELIGSLTHKLETLQEAKGSLLTDIKLNNALGEEVEALISELCKPNEFDKYRMFIGDLD
                     KVVNLLLSLSGRLARVENVLSGLGEDASNEERSSLYEKRKILAGQHEDARELKENLDR
                     RERVVLGILANYLSEEQLQDYQHFVKMKSTLLIEQRKLDDKIKLGQEQVKCLLESLPS
                     DFIPKAGALALPPNLTSEPIPAGGCTFSGIFPTLTSPL"
     exon            993..1147
                     /gene="SHROOM3"
                     /gene_synonym="APXL3; MSTP013; SHRM; ShrmL"
                     /inference="alignment:Splign:2.1.0"
     exon            1148..1279
                     /gene="SHROOM3"
                     /gene_synonym="APXL3; MSTP013; SHRM; ShrmL"
                     /inference="alignment:Splign:2.1.0"
     exon            1280..1411
                     /gene="SHROOM3"
                     /gene_synonym="APXL3; MSTP013; SHRM; ShrmL"
                     /inference="alignment:Splign:2.1.0"
     exon            1412..4577
                     /gene="SHROOM3"
                     /gene_synonym="APXL3; MSTP013; SHRM; ShrmL"
                     /inference="alignment:Splign:2.1.0"
     exon            4578..4651
                     /gene="SHROOM3"
                     /gene_synonym="APXL3; MSTP013; SHRM; ShrmL"
                     /inference="alignment:Splign:2.1.0"
     exon            4652..5533
                     /gene="SHROOM3"
                     /gene_synonym="APXL3; MSTP013; SHRM; ShrmL"
                     /inference="alignment:Splign:2.1.0"
     exon            5534..6022
                     /gene="SHROOM3"
                     /gene_synonym="APXL3; MSTP013; SHRM; ShrmL"
                     /inference="alignment:Splign:2.1.0"
     exon            6023..6173
                     /gene="SHROOM3"
                     /gene_synonym="APXL3; MSTP013; SHRM; ShrmL"
                     /inference="alignment:Splign:2.1.0"
     exon            6174..6446
                     /gene="SHROOM3"
                     /gene_synonym="APXL3; MSTP013; SHRM; ShrmL"
                     /inference="alignment:Splign:2.1.0"
     exon            6447..10891
                     /gene="SHROOM3"
                     /gene_synonym="APXL3; MSTP013; SHRM; ShrmL"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7412..7417
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SHROOM3"
                     /gene_synonym="APXL3; MSTP013; SHRM; ShrmL"
                     /note="hexamer: AATGAA"
     polyA_site      7428
                     /gene="SHROOM3"
                     /gene_synonym="APXL3; MSTP013; SHRM; ShrmL"
                     /note="major polyA site"
     regulatory      10866..10871
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SHROOM3"
                     /gene_synonym="APXL3; MSTP013; SHRM; ShrmL"
                     /note="hexamer: AATAAA"
     polyA_site      10891
                     /gene="SHROOM3"
                     /gene_synonym="APXL3; MSTP013; SHRM; ShrmL"
ORIGIN      
        1 actctttctc tcaagaggag gcaagtggct gtggcggccg cagcagtggc tgatcatcac
       61 tgaaaatacc aaagaaaaga actgagctgc ctccttcata ttttttccat tgaggattaa
      121 tttaccgtgc tttttcattt tctctacatc ctgcaaaagt ttttttctct cctaagaaac
      181 aaactatgaa ctgattgttg aaaaaaagaa gtaaaaagtt ttagcacagc ttctctgtct
      241 cttcgggaca agttagaaaa ttctgaagtg agccgaagca tagtaagtgc tttctttctt
      301 tttaagctac ttctggggag ggaggaggct attgtaatgg taaatttcac tccgagagga
      361 agaaagggtt gataatcaat caaaaatgag gtattccttt gagtatttgt gattttctta
      421 ctatattgta agatgctttt aattttctct gtaaaatagg cagaaatggt tttagtgtgt
      481 gtatgtgtga aataaaagct cagaaaagca atcttcagag cgccactgaa ggaagttttg
      541 acgaacggag tagagatgta taccacttgg gggcttcagt gagaacccag aattcctgga
      601 ggaggattta cattcagaaa tgttgaagtg aaaattcctt ctggttcagc atcttggagt
      661 tcagcttgga agaacatttt acgtatggaa gaatttgctt ctccaaacct ctcttttggc
      721 cattgtgtgt cctgaaggat gggacaactt gtgctgtaga agcactgctt gcctgagttt
      781 gcttcaggca ttttaaattt aacttgaggg atcatgtgtt tggcatgatg aggaccactg
      841 aagacttcca caagcctagt gccacattaa actctaacac ggccaccaag ggaaggtaca
      901 tttatctgga ggcattcctg gagggaggag ctccctgggg ttttactcta aagggtggcc
      961 tggagcacgg agaaccatta atcatctcta aggtcgaaga agggggcaaa gcagacaccc
     1021 tgagctccaa actgcaggct ggggatgagg ttgtgcacat caatgaggtg actctgagca
     1081 gctccagaaa ggaggcagtt tccctggtga aaggatccta caagaccctc aggctggtag
     1141 tgcgcagaga tgtgtgcaca gacccaggcc atgcagatac tggtgcctct aacttcgtca
     1201 gcccagaaca cctcacctct ggcccccagc acaggaaagc agcgtggtca ggaggggtta
     1261 aacttcggct gaagcacagg cgcagtgagc ctgcaggccg acctcactcg tggcacacaa
     1321 ctaaatctgg ggagaagcaa cccgatgcca gcatgatgca gatatctcag ggtatgatcg
     1381 gccctccttg gcaccaaagc taccattcca gctcctctac tagtgacctc tccaactatg
     1441 accatgctta tctaaggcgg agccctgacc agtgcagctc ccaggggagc atggagagcc
     1501 tggagcccag tggggcatac ccaccctgtc atctttcccc tgccaagtcc accggcagca
     1561 ttgaccagct cagccacttc cataacaaga gagactcggc ttacagctct ttctccacca
     1621 gttctagcat cctagagtat ccacaccctg gcatctctgg ccgggagcgt tcaggctcca
     1681 tggacaatac ttctgctcga ggtggcctcc tcgaagggat gaggcaggca gatattcgct
     1741 atgtcaagac agtctatgac acccggaggg gagtctcagc agagtatgag gtgaactctt
     1801 cagccctgct gcttcaaggt agggaggccc gagcctcagc aaatggtcag ggctatgata
     1861 aatggtctaa tattcctcgg ggcaagggag tgccaccccc atcctggagc cagcagtgcc
     1921 ccagttcctt ggagactgcc acggacaacc ttcctcctaa ggtgggtgca cccctgcctc
     1981 cagctcggag tgacagttac gcagcatttc ggcaccgtga gcggcccagc tcctggtcta
     2041 gccttgatca gaaacggctc tgccggcctc aggcaaactc tttaggctcc ctgaagtctc
     2101 cattcataga ggagcagctg catactgtgc tggagaagag tccagagaac agccccccag
     2161 tgaagcccaa gcataactat acccagaagg cccaacctgg ccaacctctg ctgccgacca
     2221 gcatctaccc ggtaccttcc ctggagccac actttgccca ggtgcctcag ccttctgtga
     2281 gtagcaacgg tatgctctac cctgcactgg ccaaggagag tggatacata gcccctcagg
     2341 gagcatgcaa caagatggct accattgatg agaatgggaa ccagaatgga tctggcaggc
     2401 ctgggtttgc cttctgccag cccttagaac atgacttgct gtccccagtg gagaagaaac
     2461 cagaagctac agccaagtat gtcccctcca aagtccattt ctgttcagtg cctgaaaatg
     2521 aggaggatgc ctccctgaag agacatctca cacctcccca aggcaacagc ccacattcca
     2581 atgagagaaa gagcacccac agtaacaaac catcttctca tccccacagc ctcaaatgcc
     2641 ctcaggctca ggcctggcaa gcgggtgaag acaagagatc ttccaggctc tcagagccct
     2701 gggagggcga tttccaggaa gaccacaatg ccaacctctg gaggaggctg gagagagaag
     2761 gcctaggcca gagcctgtca ggcaactttg gcaagaccaa gtcagccttc tcatctctcc
     2821 agaacattcc tgagagtctg agaagacaca gcagcctgga gctaggccgg ggaacccagg
     2881 agggttaccc cgggggcagg cccacctgtg cagtcaacac caaggcagaa gaccctggga
     2941 ggaaagccgc tcctgacctc gggagccatc tggaccggca ggtttcctac ccgcggcccg
     3001 aggggaggac cggtgcctcg gcttctttca acagcacaga cccaagtccc gaagagccgc
     3061 ctgccccctc gcacccgcac acatccagtc tgggccggag ggggcccggc ccaggcagcg
     3121 cctcggctct tcagggcttt cagtacggga agccccactg ctcggtgctg gagaaggtct
     3181 ccaaattcga gcagcgagag caagggagcc agagaccgag tgtgggcggc tctggttttg
     3241 gccataacta taggccccac aggaccgtct caacttccag tacttctggg aatgacttcg
     3301 aggagacaaa agcacacatt cgtttctctg agtcagctga acccctaggc aacggggagc
     3361 agcacttcaa aaacggggag ctgaagttgg aagaggcttc ccggcagccc tgcggtcagc
     3421 agctgagcgg aggagcgtcg gacagcggcc gtggccccca gaggccggac gctcggctcc
     3481 tccgtagcca gagcaccttc cagctctcca gcgagccaga gagggagccc gagtggcggg
     3541 acaggcccgg ctcgcccgaa tcgcccctgc tggatgcccc cttcagccgc gcctaccgga
     3601 acagcatcaa ggacgcacag tcccgtgtct tgggggccac ctcctttcga cgtcgagacc
     3661 tggagctggg ggcgcccgtg gcgtcgaggt cctggcggcc acggccttcc tcggcccacg
     3721 tggggctgcg gagccccgag gcgtcggcct ccgcctcccc gcacacgccc cgggagcggc
     3781 acagcgtgac ccctgctgag ggcgacctgg ccaggcccgt gccccctgcc gcccggagag
     3841 gtgctcgccg gcgcctgact cccgagcaga agaagcgctc ctactcggag cccgagaaga
     3901 tgaacgaggt ggggatcgtg gaggaggccg aaccggcacc cctgggcccg cagagaaatg
     3961 ggatgcgttt cccggagagc agcgtggccg accggcgccg tctcttcgag cgcgatggca
     4021 aggcctgctc cacgctcagc ctgtcggggc ccgagctgaa gcagttccag cagagcgccc
     4081 tggcggacta catccagcgc aagaccggca agcggcctac ctccgccgcc ggctgcagcc
     4141 tccaggagcc cgggccactg cgtgagcgcg cccagagtgc ctacctccag cccggccccg
     4201 cggcgctcga aggctccggc ctcgcctcgg cctccagctt gagctcactg cgggagccca
     4261 gcctgcagcc ccgcagggag gccacgctcc tgccggccac agttgcagaa acccagcagg
     4321 ctccccgaga tcgcagcagc tccttcgccg gtggccgccg cctcggggaa cggcgacgcg
     4381 gggacctgct tagcggagca aacggtggaa caaggggcac ccagagaggg gatgagaccc
     4441 ccagggagcc atcctcctgg ggggccaggg ccgggaagtc catgtcggcc gaggacctgc
     4501 tggaacgctc ggacgtcctt gcgggccctg tccatgtgag gtccaggtca tctcccgcca
     4561 ccgcagacaa gcgccaggat gtgcttttgg ggcaagacag tggctttggt cttgtgaagg
     4621 atccatgtta tttggctggt cctggatcta ggtcactcag ttgttcagaa agaggccaag
     4681 aagagatgct gccgctcttc caccatctca cccctcgttg gggtggttca ggctgcaaag
     4741 ccattggtga ttcctccgtt cctagtgaat gtcctggaac cctggaccat cagaggcaag
     4801 ccagtaggac accctgcccc aggccaccac tggcaggaac gcaagggctg gtcacagaca
     4861 ccagggctgc acccctgacc ccaattggca cccctctgcc ttcagccatt ccctctggct
     4921 actgctcaca ggacggtcag acagggcgac agcctctccc gccctacacc cctgccatga
     4981 tgcacagaag caatggtcac accctgaccc agcctcccgg tccaagaggc tgtgagggcg
     5041 atggcccaga gcatggggta gaagagggaa cgaggaagag ggtctcgctg cctcagtggc
     5101 cacctccttc tcgagcaaag tgggcccacg cagccagaga ggacagcctt cctgaggaat
     5161 cctcagcccc tgattttgca aacctgaagc actatcaaaa acagcagagt cttccaagtt
     5221 tatgcagcac ttctgaccca gacacacctc ttggggcccc gagcactcca gggaggatct
     5281 ccctccgaat atctgagtct gtcctgcggg actccccgcc acctcatgag gattatgaag
     5341 acgaagtgtt tgtgagggat ccgcacccca aggccacgtc cagccccaca tttgaacctc
     5401 ttcccccacc cccacctcct ccaccgagtc aggaaacccc ggtgtatagc atggatgact
     5461 tccctccacc tcctccccac actgtatgtg aggcgcagct ggacagtgag gatcccgagg
     5521 ggccacgccc cagcttcaac aaactttcta aagtgacaat tgcaagggaa aggcacatgc
     5581 ctggtgcagc ccatgtggta ggtagtcaga cactggcttc cagactccaa acttctatca
     5641 agggttcaga ggctgagtcc acaccaccct ccttcatgag cgttcacgcc caacttgctg
     5701 ggtctcttgg tgggcagcca gcacccatcc agactcaaag cctcagccat gatccagtca
     5761 gtggaactca gggtttagaa aagaaagtca gtcctgatcc tcagaagagt tcagaagaca
     5821 tcagaacaga ggctttggcc aaggaaattg tccaccaaga caaatctcta gcagacattt
     5881 tggatccaga ctccaggctg aagacaacaa tggacctgat ggaaggtttg tttccccgag
     5941 atgtgaactt gctgaaggaa aacagtgtaa agaggaaggc catacagaga actgtcagct
     6001 cttcaggatg tgaaggcaag aggaatgaag acaaggaagc agtgagcatg ttggttaact
     6061 gccctgccta ctacagtgtg tctgctccca aggctgagct actgaacaaa atcaaagaga
     6121 tgccagcaga agtgaatgag gaagaggaac aggcagatgt caatgaaaag aaggctgagc
     6181 tcattggaag tctcacccac aagctggaga ccctccagga ggcgaagggg agcctgctca
     6241 cggacatcaa gctcaacaac gccctgggag aagaggtgga ggctctgatc agcgagctct
     6301 gcaagcccaa tgagtttgac aagtatagga tgttcatagg ggatttggac aaggtggtca
     6361 acctgctgct ctccctctcg gggcgtctag cccgtgttga gaatgtcctt agcggccttg
     6421 gtgaagatgc cagtaatgaa gaaaggagct ctctttacga gaaaaggaag atcctggctg
     6481 gtcagcatga ggatgcccgg gagctgaagg agaacctgga tcgcagggag cgagtagtgc
     6541 tgggcatctt ggccaattac ctttcagagg agcagctcca ggactaccag cacttcgtga
     6601 aaatgaagtc cacgctcctc attgagcaac ggaagctgga tgacaagatc aagctgggcc
     6661 aggagcaggt caagtgtctg ctggagagcc tgccctcaga tttcattccc aaggctgggg
     6721 ccctggctct gcccccaaac ctcacgagtg agcccattcc tgctgggggc tgtactttca
     6781 gtggtatttt cccaacatta acctctccac tttaacctct tctaaaatac ccaaccaaaa
     6841 gatcactgtt tctctcaaca ctatttaatc tgaaaaatgt ttcagtacaa accactgttt
     6901 gaactatctg ggttattggt gtttgttcct gatgaaagga aaaaaattct ctccaggagg
     6961 aagccttttt ccttcttgcc cttcctgatt gatcttctga gagctcgaat gctgctggac
     7021 acgtacccct ttctattatt actttgtagt agaaagaaag ttaatgaaac tgagaactga
     7081 ttggagggtg tttgatcatt tagtttttaa caggctgagg caacatggat cagtgtgtgt
     7141 ccccctcagg aatgtatcca cagtggcctt ccttgctggt gggcagtgta tcctgatggc
     7201 agggtacaag taccattaat gaagggtctg caacataaag ccttaaaaag acacacacta
     7261 agaaaactgt aaaaccttga acattgttat ttatattttt taaaatggaa aagatcacta
     7321 tgtttgttgt gctaaccact tatttgattc tgttttgtgg tggacataga tgattacgtt
     7381 tgagctttgt attttgtgaa aaccttaatg aaatgaattc caaagatagt cacgttgagc
     7441 gtgaagactt tttttcttac attcctgtct tatttttacc aacacgagca ggcattctgc
     7501 gcttccactg gactgagtat gggggagtcc tgctcccctc ctgtctcaca ccttccccag
     7561 gaggaaggcc tcatgcactg cggttattgc atcatttcct agtgcttcat gtcattatca
     7621 ctggtgggga aacaaagcta aattttatta acaaagacaa ctctcagacc tatataatag
     7681 gaggaggacg tccagaatcc aacaaaattc aaacggagcc catgctgttc tcctgaacaa
     7741 gatttgggct aaatacggcc tgtttattga ggctgtttta atgatgcttt aaaaaaaaaa
     7801 aatgttttct aaaatgtcaa acctgttgac cacactttga ttaaaaactg cctccttaac
     7861 ctctgaagac tgattttgct ttatcatgtt tcaataataa catttcagag gttactctgt
     7921 agccccagtt gtaagcttat aaaaacaaac tggaaggctg aggaggttat gggctggcag
     7981 ccaggctatg tttacagctg ctggagatgg cagtagcctt atactttgag caggtagtac
     8041 atcccaggct gtgctagagg tagatttgtt ttttcacgtt tgatctgtgg ctggtggcca
     8101 cctttgttga tttgggctta cgagtttcat agtagccagt cttagaaaat tgggggagta
     8161 aaattttgaa gtaaaaaaaa aaaaaaaatg acaagggaac caaatgctcc ttctcccctt
     8221 agtacatttt cattacttaa tgaagtttta tctcctatgt aagaatatca aaattgtagt
     8281 ttgggtctcc agtagagatg tcttttgatt aacttataaa caactggcca cccagaagtt
     8341 tcctttgcat agtcaaccaa agctatggct ttgttctttg gagcttatat actgcatgac
     8401 tccattgata caaataagac agcaccttgg tttcccaggg atgttttcat tcagaggtcc
     8461 tgtctcccag gaaagcagta gaaccagtga cagcattcaa cagccacact gcatggtcat
     8521 cagtcttagg ccaacatggg agcctgaaat taactccatc agtaactacc tgtgtgtctt
     8581 gctgatactg aatgagccct cacaagccaa gccaggtgga agacagccag caaacgtcta
     8641 cagttattct tagtgaaatt cctcagagga atccaggggg ctcccagttc ccaacactct
     8701 tcgagatgac catttttcgg gatttgactg gggaacattt tacatattca gtctttctca
     8761 aaaattttta tttattttct tgagaaaggg tcttactatg ttgcccaggc tggagtgcag
     8821 agtggtgcga taacagctca ttgcagtctg aacctctgag gctcaagcca tcctcccatc
     8881 tcagcctccc aagtagctgg gaccacaggt gtgcaccact atgcttggct aattttttga
     8941 ttttttatag agacgggggt ctcgctatgt tgcccaggct ggtttcaaac tcctgggctc
     9001 aagggatcct tccgctttgg tcccagaagt gctgggatta caggcatgag ccacttcacc
     9061 cagccacttt tctaatttat agacataaaa gtaattgact cccacagcac agtaacaggc
     9121 aatgctgctt ggcatgccac aaacatctga aagcttgtga cactcctagg attgtgtcca
     9181 ttctttcatt atgtggaaac tttgttctgt tatgtgaaat gggtaaggca ccaagtgaga
     9241 ctttgaaatc taatttttag gtggatgtga tatttattgt aataggactt gacctctgta
     9301 ctttaaagaa atcactaacc aaattttcaa agtttccttt taaatgcgtt tagctagaaa
     9361 tctatgtatt tatccctttc ctattttgca ttcttctccc actattttta aaaactcatt
     9421 tacagtagaa accattcttc tttctcccaa cagtatcctt tgccaagacc atgagaacag
     9481 taaggagcat gttgttggtc agggtttcag aatacgcgtg atgtcactga gaatgtttgc
     9541 tcacagtcaa taattgtctt tgtggatgtg ataattttgg agatacactt ctggtcagaa
     9601 ctcaggtgag ataatcttgc aatactccaa atgcagatac tccagccacc cgcaaggttc
     9661 caggaaagga caatgtcctg cgagaaaatc aggaggcctc cacttcctgg gccacttgag
     9721 aagttcctgg gcatgtcact acatgttggt tgactcagcc atttctcatg ctgttttgtt
     9781 tcttgcggtg gccacttaac cccaaagaat gaagggagga tccacagtga aagtgcctga
     9841 gtttctctat gagaccagat gctgtcgaaa ccaaacatct tttcctttgc tctatgggaa
     9901 cattttaggg tttgttttgc acagctggtt tccagactag aagattaaca agtttgggtc
     9961 cacccctaag aatcagtggc tgtcttttaa ggtgaggagt gtgggcttaa ctgaggtcct
    10021 ttgagggagc tataaaggag aaacaacctg ggacatccca gttttcctat tcctccactg
    10081 ttaatatctc atctaaaata attcatgagt ttaaatggta aatatatgct ttaagctcta
    10141 cctttaaact tgtatgttat tcaggcatct cttattaaga tactgggtct ctggataccc
    10201 aaggaaatgt tggcttttta ttcttatgtg gttccaaatt tacttctctt cagtttaatt
    10261 gtccatggta gtgctggctt cattgttagg ttggagttat tattttcctt ttagaacaac
    10321 atgaatttta ttcaccttac tattagaaaa aggaataact acagccagaa ataacttcat
    10381 ttcctacaag gtataaaaag tggtcaagtg aatgtgaagg ggcttttcta cacaggaata
    10441 tattatcggg aacaaagtat ttcctgctgc cttaactctt tgggatgcat aggataaaat
    10501 gataaagacc attttaatat cagaaagggt tgtcttatta atttttaaat aaaacttcac
    10561 atttcttaat ggggagctca ttcagaaact aaataatggt ttctcaaagt gtggtcagga
    10621 tacgatctgc atcagaatcc ttggaatgct tgttaaaaat accaattgct atgacaaaac
    10681 caagtctgct ggaaactgca tttcagcagg tttcccatgt tattctgatg tattttaaca
    10741 tttgagagcc actaccaatc atctgtacag ttcctactgt tatgatcaca ataagactaa
    10801 catgaattta ataatctgaa taatttttaa aatttaaata aacagaaatt gaagttaaat
    10861 ttaaaaataa aaataacctc agaagttgaa a
//
