LOCUS       NM_001042683            7596 bp    mRNA    linear   PRI 17-SEP-2024
DEFINITION  Homo sapiens SNF2 histone linker PHD RING helicase (SHPRH),
            transcript variant 1, mRNA.
ACCESSION   NM_001042683
VERSION     NM_001042683.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7596)
  AUTHORS   Nagelberg,A.L., Sihota,T.S., Chuang,Y.C., Shi,R., Chow,J.L.M.,
            English,J., MacAulay,C., Lam,S., Lam,W.L. and Lockwood,W.W.
  TITLE     Integrative genomics identifies SHPRH as a tumor suppressor gene in
            lung adenocarcinoma that regulates DNA damage response
  JOURNAL   Br J Cancer 131 (3), 534-550 (2024)
   PUBMED   38890444
  REMARK    GeneRIF: Integrative genomics identifies SHPRH as a tumor
            suppressor gene in lung adenocarcinoma that regulates DNA damage
            response.
REFERENCE   2  (bases 1 to 7596)
  AUTHORS   Gao,J., Pan,H., Li,J., Jiang,J. and Wang,W.
  TITLE     A peptide encoded by the circular form of the SHPRH gene induces
            apoptosis in neuroblastoma cells
  JOURNAL   PeerJ 12, e16806 (2024)
   PUBMED   38282862
  REMARK    GeneRIF: A peptide encoded by the circular form of the SHPRH gene
            induces apoptosis in neuroblastoma cells.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7596)
  AUTHORS   Chen,W., Zhong,M., Yu,J., Xie,R., Zhou,T., Zhang,S., Xiong,C. and
            Huang,D.
  TITLE     KMT2B promotes SHPRH expression to regulate 131I sensitivity in
            thyroid carcinoma cells by affecting FYN protein stability
  JOURNAL   Cell Signal 88, 110165 (2021)
   PUBMED   34606908
  REMARK    GeneRIF: KMT2B promotes SHPRH expression to regulate (131)I
            sensitivity in thyroid carcinoma cells by affecting FYN protein
            stability.
REFERENCE   4  (bases 1 to 7596)
  AUTHORS   Seelinger,M., Sogaard,C.K. and Otterlei,M.
  TITLE     The Human RAD5 Homologs, HLTF and SHPRH, Have Separate Functions in
            DNA Damage Tolerance Dependent on The DNA Lesion Type
  JOURNAL   Biomolecules 10 (3), 463 (2020)
   PUBMED   32192191
  REMARK    GeneRIF: The Human RAD5 Homologs, HLTF and SHPRH, Have Separate
            Functions in DNA Damage Tolerance Dependent on The DNA Lesion Type.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 7596)
  AUTHORS   Seelinger,M. and Otterlei,M.
  TITLE     Helicase-Like Transcription Factor HLTF and E3 Ubiquitin Ligase
            SHPRH Confer DNA Damage Tolerance through Direct Interactions with
            Proliferating Cell Nuclear Antigen (PCNA)
  JOURNAL   Int J Mol Sci 21 (3), 693 (2020)
   PUBMED   31973093
  REMARK    GeneRIF: Helicase-Like Transcription Factor HLTF and E3 Ubiquitin
            Ligase SHPRH Confer DNA Damage Tolerance through Direct
            Interactions with Proliferating Cell Nuclear Antigen (PCNA).
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 7596)
  AUTHORS   Lin,J.R., Zeman,M.K., Chen,J.Y., Yee,M.C. and Cimprich,K.A.
  TITLE     SHPRH and HLTF act in a damage-specific manner to coordinate
            different forms of postreplication repair and prevent mutagenesis
  JOURNAL   Mol Cell 42 (2), 237-249 (2011)
   PUBMED   21396873
  REMARK    GeneRIF: HLTF and SHPRH suppress mutagenesis in a damage-specific
            manner, preventing mutations induced by UV rays and methyl
            methanesulfonate.
REFERENCE   7  (bases 1 to 7596)
  AUTHORS   Motegi,A., Liaw,H.J., Lee,K.Y., Roest,H.P., Maas,A., Wu,X.,
            Moinova,H., Markowitz,S.D., Ding,H., Hoeijmakers,J.H. and Myung,K.
  TITLE     Polyubiquitination of proliferating cell nuclear antigen by HLTF
            and SHPRH prevents genomic instability from stalled replication
            forks
  JOURNAL   Proc Natl Acad Sci U S A 105 (34), 12411-12416 (2008)
   PUBMED   18719106
  REMARK    GeneRIF: HLTF and SHPRH are functional homologues of yeast Rad5
            that cooperatively mediate PCNA polyubiquitination and maintain
            genomic stability.
REFERENCE   8  (bases 1 to 7596)
  AUTHORS   Motegi,A., Sood,R., Moinova,H., Markowitz,S.D., Liu,P.P. and
            Myung,K.
  TITLE     Human SHPRH suppresses genomic instability through proliferating
            cell nuclear antigen polyubiquitination
  JOURNAL   J Cell Biol 175 (5), 703-708 (2006)
   PUBMED   17130289
  REMARK    GeneRIF: We report that a putative tumor suppressor gene, SHPRH, is
            a human orthologue of yeast RAD5.The yRad5/SHPRH-dependent pathway
            is a conserved and fundamental DNA repair mechanism that protects
            the genome from genotoxic stress.
REFERENCE   9  (bases 1 to 7596)
  AUTHORS   Unk,I., Hajdu,I., Fatyol,K., Szakal,B., Blastyak,A., Bermudez,V.,
            Hurwitz,J., Prakash,L., Prakash,S. and Haracska,L.
  TITLE     Human SHPRH is a ubiquitin ligase for Mms2-Ubc13-dependent
            polyubiquitylation of proliferating cell nuclear antigen
  JOURNAL   Proc Natl Acad Sci U S A 103 (48), 18107-18112 (2006)
   PUBMED   17108083
  REMARK    GeneRIF: SHPRH function is an important deterrent to mutagenesis
            and carcinogenesis in humans
REFERENCE   10 (bases 1 to 7596)
  AUTHORS   Sood,R., Makalowska,I., Galdzicki,M., Hu,P., Eddings,E.,
            Robbins,C.M., Moses,T., Namkoong,J., Chen,S. and Trent,J.M.
  TITLE     Cloning and characterization of a novel gene, SHPRH, encoding a
            conserved putative protein with SNF2/helicase and PHD-finger
            domains from the 6q24 region
  JOURNAL   Genomics 82 (2), 153-161 (2003)
   PUBMED   12837266
  REMARK    GeneRIF: SHPRH maps to 6q24 and is a possible candidate for the
            tumor suppressor gene reported from that region.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL451145.15, AY163808.1, AY161136.1, BC117686.1, AK126959.1,
            AA315737.1 and BX648322.1.
            
            On Nov 22, 2018 this sequence version replaced NM_001042683.2.
            
            Summary: SHPRH is a ubiquitously expressed protein that contains
            motifs characteristics of several DNA repair proteins,
            transcription factors, and helicases. SHPRH is a functional homolog
            of S. cerevisiae RAD5 (Unk et al., 2006 [PubMed
            17108083]).[supplied by OMIM, Mar 2008].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC117686.1, AY161136.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000275233.12/ ENSP00000275233.7
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-261               AL451145.15        60833-61093         c
            262-309             AY163808.1         1-48
            310-3970            AY161136.1         1-3661
            3971-4851           BC117686.1         3376-4256
            4852-4955           AK126959.1         1856-1959
            4956-4956           AA315737.1         262-262
            4957-6552           AK126959.1         1961-3556
            6553-7558           AY163808.1         5956-6961
            7559-7596           BX648322.1         6431-6468
FEATURES             Location/Qualifiers
     source          1..7596
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6q24.3"
     gene            1..7596
                     /gene="SHPRH"
                     /gene_synonym="bA545I5.2; RAD5"
                     /note="SNF2 histone linker PHD RING helicase"
                     /db_xref="GeneID:257218"
                     /db_xref="HGNC:HGNC:19336"
                     /db_xref="MIM:608048"
     exon            1..628
                     /gene="SHPRH"
                     /gene_synonym="bA545I5.2; RAD5"
                     /inference="alignment:Splign:2.1.0"
     exon            629..1293
                     /gene="SHPRH"
                     /gene_synonym="bA545I5.2; RAD5"
                     /inference="alignment:Splign:2.1.0"
     CDS             661..5712
                     /gene="SHPRH"
                     /gene_synonym="bA545I5.2; RAD5"
                     /EC_number="2.3.2.27"
                     /note="isoform a is encoded by transcript variant 1;
                     2610103K11Rik; E3 ubiquitin-protein ligase SHPRH; SNF2,
                     histone-linker, PHD and RING finger domain-containing
                     helicase; SNF2 histone linker PHD RING helicase, E3
                     ubiquitin protein ligase; RING-type E3 ubiquitin
                     transferase SHPRH"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase SHPRH isoform a"
                     /protein_id="NP_001036148.2"
                     /db_xref="CCDS:CCDS43513.2"
                     /db_xref="GeneID:257218"
                     /db_xref="HGNC:HGNC:19336"
                     /db_xref="MIM:608048"
                     /translation="MSSRRKRAPPVRVDEEKRQQLHWNMHEDRRNEPIIISDDDEQPC
                     PGSDTSSAHYIILSDSLKEEVAHRDKKRCSKVVSFSKPIEKEETVGIFSPLSVKLNIV
                     ISPYHFDNSWKAFLGELTLQLLPAQSLIENFSERSITLMSSESSNQFLIYVHSKGEDV
                     EKQKKEPMSICDKGILVESSFSGEMLEDLGWLQKKRRIKLYQKPEGNHIIKVGIYLLE
                     AGLAKLDFLSDANSRMKKFNQLMKKVMEKLHNSIIPDVLEEDEDDPESEPEGQDIDEL
                     YHFVKQTHQQETQSIQVDVQHPALIPVLRPYQREAVNWMLQQECFRSSPATESALHFL
                     WREIVTSEGLKLYYNPYTGCIIREYPNSGPQLLGGILADEMGLGKTVEVLALILTHTR
                     QDVKQDALTLPEGKVVNYFIPSHYFGGKLKKTEIQNIEFEPKEKVQCPPTRVMILTAV
                     KEMNGKKGVSILSIYKYVSSIYRYDVQRNRSLLKRMLKCLIFEGLVKQIKGHGFSGTF
                     TLGKNYKEEDICDKTKKQAVGSPRKIQKETRKSGNKDTDSEYLPSDTSDDDDDPYYYY
                     YKSRRNRSKLRKKLVPSTKKGKSQPFINPDSQGHCPATSDSGITDVAMSKSTCISEFN
                     QEHETEDCAESLNHADSDVPPSNTMSPFNTSDYRFECICGELDQIDRKPRVQCLKCHL
                     WQHAKCVNYDEKNLKIKPFYCPHCLVAMEPVSTRATLIISPSSICHQWVDEINRHVRS
                     SSLRVLVYQGVKKDGFLQPHFLAEQDIVIITYDVLRSELNYVDIPHSNSEDGRRLRNQ
                     KRYMAIPSPLVAVEWWRICLDEAQMVECPTVKAAEMAQRLSGINRWCISGTPVQRGLE
                     DLFGLVVFLGIEPYCVKHWWVRLLYRPYCKKNPQHLYSFIAKILWRSAKKDVIDQIQI
                     PPQTEEIHWLHFSPVERHFYHRQHEVCCQDVVVKLRKISDWALKLSSLDRRTVTSILY
                     PLLRLRQACCHPQAVRGEFLPLQKSTMTMEELLTSLQKKCGTECEEAHRQLVCALNGL
                     AGIHIIKGEYALAAELYREVLRSSEEHKGKLKTDSLQRLHATHNLMELLIARHPGIPP
                     TLRDGRLEEEAKQLREHYMSKCNTEVAEAQQALYPVQQTIHELQRKIHSNSPWWLNVI
                     HRAIEFTIDEELVQRVRNEITSNYKQQTGKLSMSEKFRDCRGLQFLLTTQMEELNKCQ
                     KLVREAVKNLEGPPSRNVIESATVCHLRPARLPLNCCVFCKADELFTEYESKLFSNTV
                     KGQTAIFEEMIEDEEGLVDDRAPTTTRGLWAISETERSMKAILSFAKSHRFDVEFVDE
                     GSTSMDLFEAWKKEYKLLHEYWMALRNRVSAVDELAMATERLRVRDPREPKPNPPVLH
                     IIEPHEVEQNRIKLLNDKAVATSQLQKKLGQLLYLTNLEKSQDKTSGGVNPEPCPICA
                     RQLGKQWAVLTCGHCFCNECISIIIEQYSVGSHRSSIKCAICRQTTSHKEISYVFTSE
                     KANQEEDIPVKGSHSTKVEAVVRTLMKIQLRDPGAKALVFSTWQDVLDIISKALTDNN
                     MEFAQISRVKTFQENLSAFKRDPQINILLLPLHTGSNGLTIIEATHVLLVEPILNPAH
                     ELQAIGRVHRIGQTKPTIVHRFLIKATIEERMQAMLKTAERSHTNSSAKHSEASVLTV
                     ADLADLFTKETEELE"
     exon            1294..1423
                     /gene="SHPRH"
                     /gene_synonym="bA545I5.2; RAD5"
                     /inference="alignment:Splign:2.1.0"
     exon            1424..1642
                     /gene="SHPRH"
                     /gene_synonym="bA545I5.2; RAD5"
                     /inference="alignment:Splign:2.1.0"
     exon            1643..1721
                     /gene="SHPRH"
                     /gene_synonym="bA545I5.2; RAD5"
                     /inference="alignment:Splign:2.1.0"
     exon            1722..1872
                     /gene="SHPRH"
                     /gene_synonym="bA545I5.2; RAD5"
                     /inference="alignment:Splign:2.1.0"
     exon            1873..1981
                     /gene="SHPRH"
                     /gene_synonym="bA545I5.2; RAD5"
                     /inference="alignment:Splign:2.1.0"
     exon            1982..2238
                     /gene="SHPRH"
                     /gene_synonym="bA545I5.2; RAD5"
                     /inference="alignment:Splign:2.1.0"
     exon            2239..2898
                     /gene="SHPRH"
                     /gene_synonym="bA545I5.2; RAD5"
                     /inference="alignment:Splign:2.1.0"
     exon            2899..3150
                     /gene="SHPRH"
                     /gene_synonym="bA545I5.2; RAD5"
                     /inference="alignment:Splign:2.1.0"
     exon            3151..3229
                     /gene="SHPRH"
                     /gene_synonym="bA545I5.2; RAD5"
                     /inference="alignment:Splign:2.1.0"
     exon            3230..3393
                     /gene="SHPRH"
                     /gene_synonym="bA545I5.2; RAD5"
                     /inference="alignment:Splign:2.1.0"
     exon            3394..3650
                     /gene="SHPRH"
                     /gene_synonym="bA545I5.2; RAD5"
                     /inference="alignment:Splign:2.1.0"
     exon            3651..3772
                     /gene="SHPRH"
                     /gene_synonym="bA545I5.2; RAD5"
                     /inference="alignment:Splign:2.1.0"
     exon            3773..3861
                     /gene="SHPRH"
                     /gene_synonym="bA545I5.2; RAD5"
                     /inference="alignment:Splign:2.1.0"
     exon            3862..3954
                     /gene="SHPRH"
                     /gene_synonym="bA545I5.2; RAD5"
                     /inference="alignment:Splign:2.1.0"
     exon            3955..4062
                     /gene="SHPRH"
                     /gene_synonym="bA545I5.2; RAD5"
                     /inference="alignment:Splign:2.1.0"
     exon            4063..4205
                     /gene="SHPRH"
                     /gene_synonym="bA545I5.2; RAD5"
                     /inference="alignment:Splign:2.1.0"
     exon            4206..4379
                     /gene="SHPRH"
                     /gene_synonym="bA545I5.2; RAD5"
                     /inference="alignment:Splign:2.1.0"
     exon            4380..4442
                     /gene="SHPRH"
                     /gene_synonym="bA545I5.2; RAD5"
                     /inference="alignment:Splign:2.1.0"
     exon            4443..4668
                     /gene="SHPRH"
                     /gene_synonym="bA545I5.2; RAD5"
                     /inference="alignment:Splign:2.1.0"
     exon            4669..4812
                     /gene="SHPRH"
                     /gene_synonym="bA545I5.2; RAD5"
                     /inference="alignment:Splign:2.1.0"
     exon            4813..4914
                     /gene="SHPRH"
                     /gene_synonym="bA545I5.2; RAD5"
                     /inference="alignment:Splign:2.1.0"
     exon            4915..4986
                     /gene="SHPRH"
                     /gene_synonym="bA545I5.2; RAD5"
                     /inference="alignment:Splign:2.1.0"
     exon            4987..5175
                     /gene="SHPRH"
                     /gene_synonym="bA545I5.2; RAD5"
                     /inference="alignment:Splign:2.1.0"
     exon            5176..5268
                     /gene="SHPRH"
                     /gene_synonym="bA545I5.2; RAD5"
                     /inference="alignment:Splign:2.1.0"
     exon            5269..5355
                     /gene="SHPRH"
                     /gene_synonym="bA545I5.2; RAD5"
                     /inference="alignment:Splign:2.1.0"
     exon            5356..5534
                     /gene="SHPRH"
                     /gene_synonym="bA545I5.2; RAD5"
                     /inference="alignment:Splign:2.1.0"
     exon            5535..5615
                     /gene="SHPRH"
                     /gene_synonym="bA545I5.2; RAD5"
                     /inference="alignment:Splign:2.1.0"
     exon            5616..7596
                     /gene="SHPRH"
                     /gene_synonym="bA545I5.2; RAD5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7571..7576
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SHPRH"
                     /gene_synonym="bA545I5.2; RAD5"
                     /note="hexamer: AATAAA"
     polyA_site      7596
                     /gene="SHPRH"
                     /gene_synonym="bA545I5.2; RAD5"
                     /note="major polyA site"
ORIGIN      
        1 acgctacagc gcgcgcccgg cagctaggcg ctgtggtttc tgggtctccc gggggacccc
       61 tacacgttcc ggcaaaaagc gctcgtgccc acctcaagcc catgttcggt gggtggcgaa
      121 tgcgtgcccg tgatctgctg ggtctgtaga ggagcgcagg cctggtgcaa acagactcaa
      181 gaggctcgga gaaccagctt gggtaggcgc ttgggtgatg gaaggtcagg ggtcagaggt
      241 cgggagggag ggagggaggt ggtctttggc ctccgggacc agggagggag gacgctggtt
      301 gtcctggagc ccgggaagct caagggctga gagggagccg ctgcgcgagt cttccccacc
      361 tcttcccgat cccggactag cgaacttcgg accaccacgt ccctccgggg ataaagtggg
      421 gtcgtgggaa aggaggctgt gcttctcctc atgcactggg gcctgcggtg tttgtctcga
      481 gcgtccaaca cactaggatc cgggagtccg tcgtcacgct ccttggcctc gcggtcgctt
      541 gtttgctcgg cctgctggga gcgatccggc gctgacagcc ccgctcagac tccgggagcc
      601 gcttgtgggt ctttcggaag ataactgaag ttcacagtta ccagccaaga aaacttgaaa
      661 atgagcagcc gacggaaacg tgctcctcca gtgagggtag atgaggaaaa gaggcagcag
      721 cttcattgga atatgcatga ggacagaagg aatgaaccta tcatcataag tgatgatgac
      781 gagcagccct gcccaggttc agatacctct tctgctcatt atatcattct aagtgatagt
      841 ctaaaggaag aagtggctca cagagataag aagaggtgtt caaaagtggt gagcttctca
      901 aaaccaattg aaaaagaaga gactgttggt attttttccc ctttgtctgt aaagttaaat
      961 attgtgattt ctccctatca ttttgataat tcctggaaag catttctagg agaattaact
     1021 cttcagcttc ttcctgcaca gagtttaatt gaaaattttt ccgaaaggag tattacattg
     1081 atgagttcag agtcaagcaa tcagttcctg atttatgttc attcaaaagg tgaagatgta
     1141 gagaaacaaa aaaaagaacc gatgagtatt tgtgacaagg gtattctggt ggagtcatcc
     1201 ttcagtggtg aaatgttaga agatttgggg tggctacaaa agaagagaag aataaaactc
     1261 tatcagaaac cagaaggaaa tcacattatc aaggttggaa tttatctttt ggaagctggc
     1321 ctagcaaaac tagacttctt gagtgatgca aattcaagaa tgaaaaagtt caatcagctc
     1381 atgaagaaag taatggaaaa gttacacaat tctattattc cagatgtgtt ggaagaggat
     1441 gaagatgatc cggagagtga gccagaggga caagacattg acgagctgta tcactttgtg
     1501 aaacaaacac atcagcaaga aacgcagtcc atccaagtgg atgtccagca tcctgcattg
     1561 atccctgtgt tgagacccta ccaaagagag gctgtcaatt ggatgctaca acaagagtgt
     1621 ttcagaagca gtcctgctac tgaaagtgcc ctgcacttct tatggcgaga gattgttaca
     1681 tctgagggtc tgaaactcta ctataatcca tatacaggct gcatcattcg tgagtaccca
     1741 aattctgggc cgcagttgct tggtggaatt ttagcagatg agatgggtct tggaaagaca
     1801 gtggaggttt tggctctgat tctgacacat actcgacaag atgtcaagca agatgctctc
     1861 actcttcccg agggaaaagt ggtgaattat tttattccgt cacattattt tggaggaaaa
     1921 ctgaaaaaga cagaaatcca gaatatcgaa tttgaaccaa aagaaaaagt tcaatgccct
     1981 cctacacgtg tgatgatact gacagctgtg aaagaaatga atggaaaaaa aggagtgtcc
     2041 atcctttcca tctataagta tgtcagttct atatatagat acgatgttca acggaacagg
     2101 agtcttttga aacggatgct gaaatgttta atttttgaag gtctcgtgaa acagatcaaa
     2161 ggccatggtt tttctgggac ttttacattg ggaaagaatt acaaggaaga ggatatatgt
     2221 gataaaacaa aaaagcaggc agtagggagt ccaaggaaaa ttcagaaaga aactagaaaa
     2281 tcagggaaca aggacacaga ttctgaatac ttgccatcag acacctctga tgatgatgat
     2341 gatccttact attattatta taagtccagg agaaatcgca gtaaattgag gaaaaagctt
     2401 gttccatcca caaaaaaagg aaaaagtcaa ccatttatca atcccgattc acaaggtcac
     2461 tgtccagcta ctagcgactc tggaataact gatgttgcta tgtctaaaag tacatgtatc
     2521 tctgaattca accaagaaca tgaaacagag gactgtgctg aatctctaaa tcatgctgat
     2581 agtgatgtac caccttctaa taccatgagt ccctttaaca cctctgatta ccgctttgag
     2641 tgtatatgtg gtgaacttga tcagatagat cgtaagcctc gtgttcaatg cctgaagtgt
     2701 cacctgtggc aacatgcaaa gtgtgtgaat tatgatgaga aaaatctgaa gatcaagcct
     2761 ttttactgcc cccactgcct tgttgcaatg gaaccagtgt caacaagagc aactctgatc
     2821 atctctccaa gttccatctg tcaccagtgg gtggatgaga tcaacaggca tgtgaggtcg
     2881 tcatctcttc gagtcttggt atatcaagga gtgaagaaag atggcttttt acaacctcat
     2941 tttttggcag aacaggatat agttatcatt acctatgatg tactgcgttc agaattaaat
     3001 tatgtcgata tcccacatag caatagtgag gatgggcgtc gcctacggaa ccagaagcgc
     3061 tatatggcta tcccgagccc cctggtagct gtggagtggt ggaggatctg ccttgatgaa
     3121 gctcagatgg ttgagtgtcc cacagtaaaa gctgcagaaa tggcccagcg tttgagtggg
     3181 attaatcgat ggtgtatcag tggcactcca gtacagagag gattagagga tctttttggg
     3241 ttagtggtct ttcttggtat tgaaccttac tgtgtcaaac actggtgggt tcgacttctc
     3301 tatcggcctt actgcaagaa gaatcctcag catctctaca gctttattgc caagatactg
     3361 tggaggtctg caaagaaaga tgtgattgac caaatccaaa taccaccaca aaccgaagaa
     3421 atacactggc tccacttttc tccagtggaa aggcatttct atcaccgtca gcatgaggtg
     3481 tgctgccagg atgtggtggt aaaactcagg aagatttctg actgggctct gaagctcagc
     3541 agcctagaca gaaggactgt cacctctatc ctgtatccat tgctgaggct cagacaggcc
     3601 tgctgtcacc cacaggctgt tcgtggagag ttcttgccac tccaaaaaag caccatgaca
     3661 atggaagagc tgctgacatc tttgcagaag aaatgtggaa ctgaatgtga agaagcacat
     3721 cgacagctag tttgtgctct caatggctta gcaggcattc atattattaa aggtgagtat
     3781 gccttggcag cagaattgta cagagaagtg ttgcgctcct cggaggaaca caaaggaaaa
     3841 ctcaaaactg attcacttca aagacttcat gctacccata acttgatgga attgttgata
     3901 gccaggcacc cagggatacc acctaccttg cgtgatggcc gacttgagga agaggccaaa
     3961 cagctgcgag agcactacat gagcaagtgt aatacagaag ttgctgaagc ccagcaagct
     4021 ttatatcctg tgcagcagac catccatgag cttcaaagaa agattcattc taattctcct
     4081 tggtggctaa atgtgatcca cagagcaata gaatttacta ttgatgagga gctagttcag
     4141 cgagtgcgaa atgaaataac cagcaactac aagcaacaaa ctggcaagct ttctatgtca
     4201 gagaagttcc gtgattgcag aggtcttcag ttcttactta caacacaaat ggaagagcta
     4261 aataaatgcc agaagctagt aagagaggct gtaaaaaacc tggagggacc tccatctcgt
     4321 aatgttattg agtctgcaac agtctgtcac ctccgaccag ccagacttcc tctcaactgc
     4381 tgtgtctttt gtaaagctga tgaattgttc acagagtatg aatcaaagct attttccaac
     4441 acagtcaaag gccagactgc aatatttgag gagatgatag aagatgaaga aggactggtg
     4501 gatgatcgag cacctaccac cacccggggt ctctgggcaa taagtgagac agagcgatct
     4561 atgaaagcaa tactatcatt tgcaaaatca cataggtttg atgttgaatt tgttgatgaa
     4621 ggaagcactt caatggatct ctttgaagca tggaagaagg aatataagtt gcttcatgaa
     4681 tattggatgg ctctgaggaa tcgtgtgtct gctgttgatg aacttgcaat ggctacagaa
     4741 cgactaagag tgcgtgatcc tagggagcca aagcctaatc cgcctgttct tcatatcatt
     4801 gaaccacatg aggtagaaca aaaccgaata aaactactaa atgataaagc tgttgctaca
     4861 tcacagcttc agaaaaaact tgggcagctt ctttacctaa ctaatttgga gaagtctcaa
     4921 gataaaacat cgggaggtgt taatccagaa ccttgcccaa tctgtgctcg acagctagga
     4981 aaacagtggg cggtactgac ctgtggtcac tgtttctgta atgaatgcat ttctataatt
     5041 attgaacaat acagcgtggg atctcacaga agctccatta agtgtgcaat ctgccgccag
     5101 accacatctc acaaagaaat ctcgtatgtc tttacctcag agaaagcaaa ccaggaggag
     5161 gacatccctg tgaagggcag ccattctaca aaagtggaag ctgtggtcag aactctgatg
     5221 aaaatacagc ttagagatcc aggggccaaa gcactcgttt tctcaacgtg gcaagatgta
     5281 ttagatatta tttcaaaagc tcttactgac aacaacatgg aatttgcaca aatcagtcgt
     5341 gttaagacat ttcaggagaa cctttcagca tttaaacgtg atccccaaat caatattttg
     5401 ctgctgcccc tgcacacagg ttctaatgga ttaactatca ttgaagcaac tcatgttctc
     5461 ttggtggagc ccatattgaa ccctgcccat gagcttcagg ccatagggag ggtgcaccga
     5521 attggacaga caaaacctac tattgtacac agattcttaa ttaaagcaac aatagaagaa
     5581 agaatgcagg caatgctgaa aactgctgag agaagtcaca cgaactcatc agcaaagcat
     5641 tcagaggcct ctgtcttgac tgtggctgac ctggcagacc tatttaccaa agaaactgaa
     5701 gagcttgaat gaactacact tgattcattc catggacttt agtgtattaa taaactttca
     5761 tagctgtaga gcaaagttac aagttttaaa aacccagtag ataacagtaa cactgttcct
     5821 agttgaatga atttgtttat tgttctggtt atacagtggc ttagtactta ttgagtcctt
     5881 ttcaatatac tgtttccaaa agatctataa agattcttaa ttttacactc ctaataaaac
     5941 atttattttt tgtcccaaat agtatctata tctgatgatg caagggaatt aagagaggta
     6001 atgtgtactt ttcttatgat aagatcactc ataaaacagt tggaataaga aagtaaacta
     6061 gtctagtttt atatgacctg gtatattgaa caaacataga tctcagtttt ggaagggcct
     6121 tcagtatctt tagcacatga ttcaccaata tcttgttagt aatattttaa gtagatggtg
     6181 aattatatta acccattttt tgagtaggct tataccataa ctttacacct agtaattttg
     6241 cagtaataca actccttaca ataagttaat gttagacagt tatttgtttc ttctgcatct
     6301 tgctactgaa aacaaaaaca atgaaaatat tcatatttct attatgtgta aagtggtgtt
     6361 agtgtaaaca gttaactcca aatagatttt ttaatgtatt aatgattacc ttaaaattat
     6421 ctgctttccc tactcttaat ttttcttatt gttaaagttt ttatatgttg taagattgat
     6481 gtatctgtaa atattgcagt ttttatgatt ttgttttatt ataacatgga aatatggcag
     6541 ggtttttttt ttaatcatta ttatactgat gtcttggatt ctttttcttt cccatgttct
     6601 tgtgtaacta gactttttag ccagtgatat ctgaattcat ggtagagttg ttatttcact
     6661 ccagttgttc ctcccttaag cacgagtctt tgaccttttg aacaagagaa tgccatgttc
     6721 acataagcaa atcgttgcaa gtgataataa cgaagtgttt cttcttctgt gaggaattaa
     6781 tggactttga aattgctgaa taaggagtag cttgttacat cctagccatt tgatgtaata
     6841 caaatgttct tatttggctt ccttttcttt gagccttcag agttttaaag gaccctcttt
     6901 gaccctcagg acttattagc tcccagttag ctgcctggaa aactcataga accattccct
     6961 agatcccatt tgtccgagag gctttggtgc ttagttaagg tgcattttct tactctaggt
     7021 ttatagagtg atgtaattca actgaagaaa acacacacat gcttttggtg cttattcttc
     7081 agttaaaagg aggtatccat tgattctttt atatgaaatt aaatcaaact gttattttta
     7141 tagctcttca catattaaaa agagctttca tgtacattta cgtgtttaat ttacccaaca
     7201 atcttgtgaa gttgatataa ttaatatctt tactgtttta aaggtagaga actgaagttc
     7261 agatatattg aaactttctt aggacatgta gcaagtaaac agtggagccc aggtcccctg
     7321 actccatatc ttacattctt ttactaataa catgtcatgc tgagattcag aaggcccatg
     7381 cttataattt aaaaatgaga ctatttcagt atttctttgt gtttgttttc tactattcct
     7441 ttctgagcta tgttttagag aaaacaattt gatatgcttc aattttgtaa aatcaaatta
     7501 catatatata aaaaaaccta tctgtattag acaaaaactg tttttattta tttctgtaag
     7561 atatccatta aataaatatt ttagtggaaa tgaaaa
//
