LOCUS       NM_001365341            1541 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens shieldin complex subunit 3 (SHLD3), mRNA.
ACCESSION   NM_001365341
VERSION     NM_001365341.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Zhao,F., Kim,W., Gao,H., Liu,C., Zhang,Y., Chen,Y., Deng,M.,
            Zhou,Q., Huang,J., Hu,Q., Chen,S.H., Nowsheen,S., Kloeber,J.A.,
            Qin,B., Yin,P., Tu,X., Guo,G., Qin,S., Zhang,C., Gao,M., Luo,K.,
            Liu,Y., Lou,Z. and Yuan,J.
  TITLE     ASTE1 promotes shieldin-complex-mediated DNA repair by attenuating
            end resection
  JOURNAL   Nat Cell Biol 23 (8), 894-904 (2021)
   PUBMED   34354233
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Dai,Y., Zhang,F., Wang,L., Shan,S., Gong,Z. and Zhou,Z.
  TITLE     Structural basis for shieldin complex subunit 3-mediated
            recruitment of the checkpoint protein REV7 during DNA double-strand
            break repair
  JOURNAL   J Biol Chem 295 (1), 250-262 (2020)
   PUBMED   31796627
  REMARK    GeneRIF: Structural basis for shieldin complex subunit 3-mediated
            recruitment of the checkpoint protein REV7 during DNA double-strand
            break repair.
REFERENCE   3  (bases 1 to 1541)
  AUTHORS   Ghezraoui,H., Oliveira,C., Becker,J.R., Bilham,K., Moralli,D.,
            Anzilotti,C., Fischer,R., Deobagkar-Lele,M., Sanchiz-Calvo,M.,
            Fueyo-Marcos,E., Bonham,S., Kessler,B.M., Rottenberg,S.,
            Cornall,R.J., Green,C.M. and Chapman,J.R.
  TITLE     53BP1 cooperation with the REV7-shieldin complex underpins DNA
            structure-specific NHEJ
  JOURNAL   Nature 560 (7716), 122-127 (2018)
   PUBMED   30046110
REFERENCE   4  (bases 1 to 1541)
  AUTHORS   Noordermeer,S.M., Adam,S., Setiaputra,D., Barazas,M., Pettitt,S.J.,
            Ling,A.K., Olivieri,M., Alvarez-Quilon,A., Moatti,N.,
            Zimmermann,M., Annunziato,S., Krastev,D.B., Song,F., Brandsma,I.,
            Frankum,J., Brough,R., Sherker,A., Landry,S., Szilard,R.K.,
            Munro,M.M., McEwan,A., Goullet de Rugy,T., Lin,Z.Y., Hart,T.,
            Moffat,J., Gingras,A.C., Martin,A., van Attikum,H., Jonkers,J.,
            Lord,C.J., Rottenberg,S. and Durocher,D.
  TITLE     The shieldin complex mediates 53BP1-dependent DNA repair
  JOURNAL   Nature 560 (7716), 117-121 (2018)
   PUBMED   30022168
REFERENCE   5  (bases 1 to 1541)
  AUTHORS   Mirman,Z., Lottersberger,F., Takai,H., Kibe,T., Gong,Y., Takai,K.,
            Bianchi,A., Zimmermann,M., Durocher,D. and de Lange,T.
  TITLE     53BP1-RIF1-shieldin counteracts DSB resection through CST- and
            Polalpha-dependent fill-in
  JOURNAL   Nature 560 (7716), 112-116 (2018)
   PUBMED   30022158
REFERENCE   6  (bases 1 to 1541)
  AUTHORS   Dev,H., Chiang,T.W., Lescale,C., de Krijger,I., Martin,A.G.,
            Pilger,D., Coates,J., Sczaniecka-Clift,M., Wei,W., Ostermaier,M.,
            Herzog,M., Lam,J., Shea,A., Demir,M., Wu,Q., Yang,F., Fu,B.,
            Lai,Z., Balmus,G., Belotserkovskaya,R., Serra,V., O'Connor,M.J.,
            Bruna,A., Beli,P., Pellegrini,L., Caldas,C., Deriano,L.,
            Jacobs,J.J.L., Galanty,Y. and Jackson,S.P.
  TITLE     Shieldin complex promotes DNA end-joining and counters homologous
            recombination in BRCA1-null cells
  JOURNAL   Nat Cell Biol 20 (8), 954-965 (2018)
   PUBMED   30022119
REFERENCE   7  (bases 1 to 1541)
  AUTHORS   Gupta,R., Somyajit,K., Narita,T., Maskey,E., Stanlie,A., Kremer,M.,
            Typas,D., Lammers,M., Mailand,N., Nussenzweig,A., Lukas,J. and
            Choudhary,C.
  TITLE     DNA Repair Network Analysis Reveals Shieldin as a Key Regulator of
            NHEJ and PARP Inhibitor Sensitivity
  JOURNAL   Cell 173 (4), 972-988 (2018)
   PUBMED   29656893
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC008560.6.
            
            On Aug 31, 2019 this sequence version replaced NM_001365341.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853563.26410.1,
                                           SRR14038191.1379116.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000510585.3/ ENSP00000424007.2
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-80                AC008560.6         57981-58060         c
            81-1541             AC008560.6         52159-53619         c
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q12.3"
     gene            1..1541
                     /gene="SHLD3"
                     /gene_synonym="CTC-534A2.2; RINN1"
                     /note="shieldin complex subunit 3"
                     /db_xref="GeneID:112441434"
                     /db_xref="HGNC:HGNC:53826"
                     /db_xref="MIM:618030"
     exon            1..80
                     /gene="SHLD3"
                     /gene_synonym="CTC-534A2.2; RINN1"
                     /inference="alignment:Splign:2.1.0"
     exon            81..1541
                     /gene="SHLD3"
                     /gene_synonym="CTC-534A2.2; RINN1"
                     /inference="alignment:Splign:2.1.0"
     CDS             201..953
                     /gene="SHLD3"
                     /gene_synonym="CTC-534A2.2; RINN1"
                     /note="REV7-interacting novel NHEJ regulator 1; shield
                     complex subunit 3"
                     /codon_start=1
                     /product="shieldin complex subunit 3"
                     /protein_id="NP_001352270.1"
                     /db_xref="CCDS:CCDS93722.1"
                     /db_xref="GeneID:112441434"
                     /db_xref="HGNC:HGNC:53826"
                     /db_xref="MIM:618030"
                     /translation="MTTEVILHYRPCESDPTQLPKIAEKAIQDFPTRPLSRFIPWFPY
                     DGSKLPLRPKRSPPVISEEAAEDVKQYLTISEHDAKSHSYDCTVDLLEFQPSLKKQHL
                     TWSHTLKEQTNSGNLGKQSEKGKQHKRRSWSISLPSNNCTKNVSPLSKKLQDSLKALN
                     LHSLYRARWTIEHTICNSQTLEDIWTKLNQIIRHNELPSCNATIQRHLGQIWVFCDIM
                     YCEYVGSLLKGRLALTGKINLFVHKYGVIFSM"
     regulatory      1297..1302
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SHLD3"
                     /gene_synonym="CTC-534A2.2; RINN1"
                     /note="hexamer: AATAAA"
     polyA_site      1315
                     /gene="SHLD3"
                     /gene_synonym="CTC-534A2.2; RINN1"
                     /note="major polyA site"
     regulatory      1520..1525
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SHLD3"
                     /gene_synonym="CTC-534A2.2; RINN1"
                     /note="hexamer: AATACA"
     polyA_site      1541
                     /gene="SHLD3"
                     /gene_synonym="CTC-534A2.2; RINN1"
ORIGIN      
        1 ccccgtaggc tgtgggtcaa aagtgccggt caaaatggaa gtgaatcccc ctaaacagga
       61 gcacctgctg gcgctaaaag gtcctgtttc cctctgattt ggcaagagag acaatcttgc
      121 aataataaat taacattcta gctctgagga gttcaactaa gaaattttct catcactaag
      181 agtaactgga ttactgcaga atgactacag aagtaatatt acattatcga ccatgtgaga
      241 gtgatcccac acaactgcca aaaattgcag aaaaagcaat tcaagacttt cctactcgtc
      301 cgctatcaag atttatacct tggtttccat atgatgggtc caagcttcca ctcagaccta
      361 aaagatcacc acctgtgatt tctgaagagg cagctgaaga tgtgaaacag tacttaacca
      421 tttcagaaca tgatgctaag tcacacagtt atgattgcac agtagatcta ttggagtttc
      481 aacctagctt gaaaaagcag catttaacct ggtcacacac actgaaggaa cagactaatt
      541 ctggaaatct gggtaaacaa tcagaaaagg gaaaacagca caagaggaga tcttggagta
      601 tttcccttcc cagcaataat tgtactaaaa acgtttctcc tttgtctaaa aaattgcaag
      661 atagtttaaa ggcactaaat ttacactcac tttatagagc aagatggaca atagaacaca
      721 ctatttgtaa cagccaaact ctggaagaca tttggacaaa actcaatcaa attattaggc
      781 acaatgaact tccatcttgt aatgctacaa ttcagaggca tttaggccaa atatgggtgt
      841 tctgtgatat tatgtattgt gaatatgtgg gaagtcttct taaaggaaga ttagctctta
      901 ctggaaaaat taatttattt gtgcataaat atggtgttat ttttagtatg taatgaattc
      961 cactgattgt caaaaagaat attctgaatg aaatgaatat ggattgaaat agataaaata
     1021 atttttacag gttttaaaat ttttaatgac tttttagaat tcctaggctt gtcaattaag
     1081 tcaagaaatt gctgagttct gcttattggc agccttcttt taaatgtgat aaattattgt
     1141 ttacactgaa tttgaaggta aaaatgttct gttccttttg ttacaaactg tgattgaatt
     1201 agtcatgaat taagttgtat agtttggatt ttggttaatc tctaaaatct aattttactg
     1261 tatttttttc cttacctcaa gtgtaatttt ttaaaaaata aaactcgaaa caattctctt
     1321 tggagtgcta tttatgtggc atgaatgtag ttcaaagtgg gagactagtc tagatttata
     1381 atgatgtttc caaagatatg taataacaat tgataggctg ttttcaaaca acgatacaaa
     1441 atgtatactt tgcctaaaac gataatgtat cctgttttga aggaaagtat caaatgttga
     1501 atgattgttt atttcttcta atacagataa ttcagtatta a
//
