LOCUS       NM_152504               1633 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens shieldin complex subunit 1 (SHLD1), transcript variant
            2, mRNA.
ACCESSION   NM_152504
VERSION     NM_152504.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   de Krijger,I., Fohr,B., Perez,S.H., Vincendeau,E., Serrat,J.,
            Thouin,A.M., Susvirkar,V., Lescale,C., Paniagua,I., Hoekman,L.,
            Kaur,S., Altelaar,M., Deriano,L., Faesen,A.C. and Jacobs,J.J.L.
  TITLE     MAD2L2 dimerization and TRIP13 control shieldin activity in DNA
            repair
  JOURNAL   Nat Commun 12 (1), 5421 (2021)
   PUBMED   34521823
  REMARK    GeneRIF: MAD2L2 dimerization and TRIP13 control shieldin activity
            in DNA repair.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Zhao,F., Kim,W., Gao,H., Liu,C., Zhang,Y., Chen,Y., Deng,M.,
            Zhou,Q., Huang,J., Hu,Q., Chen,S.H., Nowsheen,S., Kloeber,J.A.,
            Qin,B., Yin,P., Tu,X., Guo,G., Qin,S., Zhang,C., Gao,M., Luo,K.,
            Liu,Y., Lou,Z. and Yuan,J.
  TITLE     ASTE1 promotes shieldin-complex-mediated DNA repair by attenuating
            end resection
  JOURNAL   Nat Cell Biol 23 (8), 894-904 (2021)
   PUBMED   34354233
REFERENCE   3  (bases 1 to 1633)
  AUTHORS   Isobe,S.Y., Hiraga,S.I., Nagao,K., Sasanuma,H., Donaldson,A.D. and
            Obuse,C.
  TITLE     Protein phosphatase 1 acts as a RIF1 effector to suppress DSB
            resection prior to Shieldin action
  JOURNAL   Cell Rep 36 (2), 109383 (2021)
   PUBMED   34260925
  REMARK    GeneRIF: Protein phosphatase 1 acts as a RIF1 effector to suppress
            DSB resection prior to Shieldin action.
REFERENCE   4  (bases 1 to 1633)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   5  (bases 1 to 1633)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 1633)
  AUTHORS   Ghezraoui,H., Oliveira,C., Becker,J.R., Bilham,K., Moralli,D.,
            Anzilotti,C., Fischer,R., Deobagkar-Lele,M., Sanchiz-Calvo,M.,
            Fueyo-Marcos,E., Bonham,S., Kessler,B.M., Rottenberg,S.,
            Cornall,R.J., Green,C.M. and Chapman,J.R.
  TITLE     53BP1 cooperation with the REV7-shieldin complex underpins DNA
            structure-specific NHEJ
  JOURNAL   Nature 560 (7716), 122-127 (2018)
   PUBMED   30046110
REFERENCE   7  (bases 1 to 1633)
  AUTHORS   Noordermeer,S.M., Adam,S., Setiaputra,D., Barazas,M., Pettitt,S.J.,
            Ling,A.K., Olivieri,M., Alvarez-Quilon,A., Moatti,N.,
            Zimmermann,M., Annunziato,S., Krastev,D.B., Song,F., Brandsma,I.,
            Frankum,J., Brough,R., Sherker,A., Landry,S., Szilard,R.K.,
            Munro,M.M., McEwan,A., Goullet de Rugy,T., Lin,Z.Y., Hart,T.,
            Moffat,J., Gingras,A.C., Martin,A., van Attikum,H., Jonkers,J.,
            Lord,C.J., Rottenberg,S. and Durocher,D.
  TITLE     The shieldin complex mediates 53BP1-dependent DNA repair
  JOURNAL   Nature 560 (7716), 117-121 (2018)
   PUBMED   30022168
REFERENCE   8  (bases 1 to 1633)
  AUTHORS   Mirman,Z., Lottersberger,F., Takai,H., Kibe,T., Gong,Y., Takai,K.,
            Bianchi,A., Zimmermann,M., Durocher,D. and de Lange,T.
  TITLE     53BP1-RIF1-shieldin counteracts DSB resection through CST- and
            Polalpha-dependent fill-in
  JOURNAL   Nature 560 (7716), 112-116 (2018)
   PUBMED   30022158
REFERENCE   9  (bases 1 to 1633)
  AUTHORS   Dev,H., Chiang,T.W., Lescale,C., de Krijger,I., Martin,A.G.,
            Pilger,D., Coates,J., Sczaniecka-Clift,M., Wei,W., Ostermaier,M.,
            Herzog,M., Lam,J., Shea,A., Demir,M., Wu,Q., Yang,F., Fu,B.,
            Lai,Z., Balmus,G., Belotserkovskaya,R., Serra,V., O'Connor,M.J.,
            Bruna,A., Beli,P., Pellegrini,L., Caldas,C., Deriano,L.,
            Jacobs,J.J.L., Galanty,Y. and Jackson,S.P.
  TITLE     Shieldin complex promotes DNA end-joining and counters homologous
            recombination in BRCA1-null cells
  JOURNAL   Nat Cell Biol 20 (8), 954-965 (2018)
   PUBMED   30022119
REFERENCE   10 (bases 1 to 1633)
  AUTHORS   Gupta,R., Somyajit,K., Narita,T., Maskey,E., Stanlie,A., Kremer,M.,
            Typas,D., Lammers,M., Mailand,N., Nussenzweig,A., Lukas,J. and
            Choudhary,C.
  TITLE     DNA Repair Network Analysis Reveals Shieldin as a Key Regulator of
            NHEJ and PARP Inhibitor Sensitivity
  JOURNAL   Cell 173 (4), 972-988 (2018)
   PUBMED   29656893
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK057796.1, AL390028.11, AK292708.1 and HY270691.1.
            
            On Nov 22, 2018 this sequence version replaced NM_152504.3.
            
            Transcript Variant: This variant (2) lacks an exon in the 5' UTR
            compared to variant 1. Variants 1 and 2 encode isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK292708.1, SRR1803613.172210.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000303142.11/ ENSP00000305875.6
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-403               AK057796.1         12-414
            404-404             AL390028.11        21121-21121
            405-1612            AK292708.1         405-1612
            1613-1633           HY270691.1         42-62               c
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20p12.3"
     gene            1..1633
                     /gene="SHLD1"
                     /gene_synonym="C20orf196; RINN3"
                     /note="shieldin complex subunit 1"
                     /db_xref="GeneID:149840"
                     /db_xref="HGNC:HGNC:26318"
                     /db_xref="MIM:618028"
     exon            1..79
                     /gene="SHLD1"
                     /gene_synonym="C20orf196; RINN3"
                     /inference="alignment:Splign:2.1.0"
     exon            80..261
                     /gene="SHLD1"
                     /gene_synonym="C20orf196; RINN3"
                     /inference="alignment:Splign:2.1.0"
     CDS             84..701
                     /gene="SHLD1"
                     /gene_synonym="C20orf196; RINN3"
                     /note="isoform 1 is encoded by transcript variant 2;
                     uncharacterized protein C20orf196; RINN1-REV7-interacting
                     novel NHEJ regulator 3; shield complex subunit 1"
                     /codon_start=1
                     /product="shieldin complex subunit 1 isoform 1"
                     /protein_id="NP_689717.2"
                     /db_xref="CCDS:CCDS13091.1"
                     /db_xref="GeneID:149840"
                     /db_xref="HGNC:HGNC:26318"
                     /db_xref="MIM:618028"
                     /translation="MAARDATSGSLSEESSALDLPSACDIRDYVLQGPSQEANSEAFS
                     SLEFHSFPYSSDVDPDTSNLNIEQNNSWTAENFWLDPAVKGQSEKEEDDGLRKSLDRF
                     YEMFGHPQPGSANSLSASVCKCLSQKITQLRGQESQKYALRSFQMARVIFNRDGCSVL
                     QRHSRDTHFYPLEEGSTSLDDEKPNPGLSKDITHFLLQQNVMKDL"
     exon            262..1633
                     /gene="SHLD1"
                     /gene_synonym="C20orf196; RINN3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1130..1135
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SHLD1"
                     /gene_synonym="C20orf196; RINN3"
                     /note="hexamer: AATAAA"
     polyA_site      1150
                     /gene="SHLD1"
                     /gene_synonym="C20orf196; RINN3"
                     /note="major polyA site"
     regulatory      1614..1619
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SHLD1"
                     /gene_synonym="C20orf196; RINN3"
                     /note="hexamer: AATAAA"
     polyA_site      1633
                     /gene="SHLD1"
                     /gene_synonym="C20orf196; RINN3"
ORIGIN      
        1 gttccggttg cggatggtga gtgtgtcggg gggcttggcg gcttttctcc tcagttttct
       61 ggagtgcttt ggaggagagg actatggcag ccagggacgc cacttcaggc agcctgtcag
      121 aggagagcag tgctttggac ctgccatcag cgtgtgacat aagagattac gtcctgcagg
      181 gacccagcca agaagccaac agcgaggctt tcagttcttt ggaattccat tcttttcctt
      241 attcttctga tgtggatcca gacaccagta acttaaatat agaacaaaat aactcctgga
      301 ccgctgagaa cttctggctt gaccctgctg tgaaaggcca gtcagagaag gaagaggatg
      361 atggccttcg gaaatccctg gatagattct atgaaatgtt tggtcatcca cagccaggct
      421 ctgcaaactc actctctgca tctgtctgca agtgcctgtc tcagaaaatc actcaactaa
      481 gaggccagga gagccaaaag tatgccctcc gcagttttca aatggcccgg gtgatcttca
      541 accgggacgg ctgctccgtc ttacagaggc attccaggga cacccacttc tacccactgg
      601 aggaaggaag tacatctttg gatgatgaaa agccaaaccc aggactgtca aaggatatta
      661 ctcatttcct cttgcagcag aatgtaatga aagacctgta actggtgccg ggcagtgtgc
      721 agggtagtaa tggaggtgct gtgccatgac cagcagtgtt ggtggccacc cagatcccct
      781 agggtctctg gccagctctg tgtgcccaat cccaattaag tgctttgagg ttaaaggctg
      841 gcacctgtga cctggtattg gagccagtca gcccatatgg agagccagca gggtctggga
      901 gttctccgtc ctcttggcca aggccgctga ctgactgggc tacgagtcaa aagcccagct
      961 cccttgcctc aaggaaggac attctctgag gagtaattta tgctctagca ctccctttcc
     1021 tctagatcgg cctgaggctg ggacattaca tgaaatcaca cccttgctgg gcttaatccc
     1081 tttccctacc ctcccattta tttactggtg tctctgggag cctttcctta ataaacctct
     1141 tatatctgaa tccttgactc agagtctact tctgggagaa ttcaccctga gagaacatgc
     1201 ttctgtgaga tgaggtttta gaaggataca aagctctaca ccgaatcagg tataaggaat
     1261 tatatcaagt tgtaaaactg agaccatgtc attgagaata catgtcctaa aataatataa
     1321 agattgaaat acagttgaga aaagtatatc acatcttgat cacacccttg cctcctctcc
     1381 aaagctagaa atccatgttt tatcatacta actgggtgtg gaaggaacat ttggacaatc
     1441 taaaataaca gcgaaaattc acaattcttt ttaaaatctc ttgtctttta tcagattgca
     1501 aacgtgaaag gtgagaaggt aacaaatgtg caccacccac cacagtatat gtaagcacaa
     1561 ttatgctacg ctaggctgat gccacgtggc cttgtcaagt tgtgtttgaa gagaataaat
     1621 aatcctttgc cca
//
