LOCUS       NM_001272067            2266 bp    mRNA    linear   PRI 22-SEP-2024
DEFINITION  Homo sapiens shisa family member 5 (SHISA5), transcript variant 4,
            mRNA.
ACCESSION   NM_001272067
VERSION     NM_001272067.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Kim,N., Kim,M.J., Sung,P.S., Bae,Y.C., Shin,E.C. and Yoo,J.Y.
  TITLE     Interferon-inducible protein SCOTIN interferes with HCV replication
            through the autolysosomal degradation of NS5A
  JOURNAL   Nat Commun 7, 10631 (2016)
   PUBMED   26868272
  REMARK    GeneRIF: IFN-beta-induced SCOTIN recruits the HCV NS5A protein to
            autophagosomes for degradation, thereby restricting HCV
            replication.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2266)
  AUTHORS   So,H.C., Fong,P.Y., Chen,R.Y., Hui,T.C., Ng,M.Y., Cherny,S.S.,
            Mak,W.W., Cheung,E.F., Chan,R.C., Chen,E.Y., Li,T. and Sham,P.C.
  TITLE     Identification of neuroglycan C and interacting partners as
            potential susceptibility genes for schizophrenia in a Southern
            Chinese population
  JOURNAL   Am J Med Genet B Neuropsychiatr Genet 153B (1), 103-113 (2010)
   PUBMED   19367581
  REMARK    GeneRIF: Observational study of gene-disease association and
            gene-gene interaction. (HuGE Navigator)
REFERENCE   4  (bases 1 to 2266)
  AUTHORS   Draeby,I., Woods,Y.L., la Cour,J.M., Mollerup,J., Bourdon,J.C. and
            Berchtold,M.W.
  TITLE     The calcium binding protein ALG-2 binds and stabilizes Scotin, a
            p53-inducible gene product localized at the endoplasmic reticulum
            membrane
  JOURNAL   Arch Biochem Biophys 467 (1), 87-94 (2007)
   PUBMED   17889823
  REMARK    GeneRIF: ALG-2 binding to Scotin is strictly calcium dependent,
            indicating a role of this interaction in calcium signaling pathways
REFERENCE   5  (bases 1 to 2266)
  AUTHORS   Ludes-Meyers,J.H., Kil,H., Bednarek,A.K., Drake,J., Bedford,M.T.
            and Aldaz,C.M.
  TITLE     WWOX binds the specific proline-rich ligand PPXY: identification of
            candidate interacting proteins
  JOURNAL   Oncogene 23 (29), 5049-5055 (2004)
   PUBMED   15064722
REFERENCE   6  (bases 1 to 2266)
  AUTHORS   Matsuda,A., Suzuki,Y., Honda,G., Muramatsu,S., Matsuzaki,O.,
            Nagano,Y., Doi,T., Shimotohno,K., Harada,T., Nishida,E., Hayashi,H.
            and Sugano,S.
  TITLE     Large-scale identification and characterization of human genes that
            activate NF-kappaB and MAPK signaling pathways
  JOURNAL   Oncogene 22 (21), 3307-3318 (2003)
   PUBMED   12761501
REFERENCE   7  (bases 1 to 2266)
  AUTHORS   Bourdon,J.C., Renzing,J., Robertson,P.L., Fernandes,K.N. and
            Lane,D.P.
  TITLE     Scotin, a novel p53-inducible proapoptotic protein located in the
            ER and the nuclear membrane
  JOURNAL   J Cell Biol 158 (2), 235-246 (2002)
   PUBMED   12135983
  REMARK    GeneRIF: Scotin plays a role in p53-dependent apoptosis
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA657994.1, DA020280.1,
            AK056328.1, BX336842.2 and BM929955.1.
            
            On Nov 19, 2020 this sequence version replaced NM_001272067.1.
            
            Summary: This gene encodes a member of the shisa family. The
            encoded protein is localized to the endoplasmic reticulum, and
            together with p53 induces apoptosis in a caspase-dependent manner.
            Alternative splicing results in multiple transcript variants.
            Related pseudogenes of this gene are found on chromosome X.
            [provided by RefSeq, Apr 2016].
            
            Transcript Variant: This variant (4) uses an alternate 5' exon and
            initiates translation at a downstream AUG, compared to variant 1.
            The encoded isoform (c) is shorter than isoform a. Variants 3, 4
            and 5 encode the same protein.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038195.2070024.1,
                                           SRR14038191.2760408.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2155770 [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2                 DA657994.1         4-5
            3-15                DA020280.1         7-19
            16-388              AK056328.1         1-373
            389-1023            BX336842.2         115-749
            1024-2265           AK056328.1         1009-2250
            2266-2266           BM929955.1         321-321
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p21.31"
     gene            1..2266
                     /gene="SHISA5"
                     /gene_synonym="SCOTIN"
                     /note="shisa family member 5"
                     /db_xref="GeneID:51246"
                     /db_xref="HGNC:HGNC:30376"
                     /db_xref="MIM:607290"
     exon            1..388
                     /gene="SHISA5"
                     /gene_synonym="SCOTIN"
                     /inference="alignment:Splign:2.1.0"
     exon            389..545
                     /gene="SHISA5"
                     /gene_synonym="SCOTIN"
                     /inference="alignment:Splign:2.1.0"
     CDS             406..1035
                     /gene="SHISA5"
                     /gene_synonym="SCOTIN"
                     /note="isoform c is encoded by transcript variant 4;
                     putative NF-kappa-B-activating protein 120; shisa homolog
                     5"
                     /codon_start=1
                     /product="protein shisa-5 isoform c"
                     /protein_id="NP_001258996.1"
                     /db_xref="CCDS:CCDS63622.1"
                     /db_xref="GeneID:51246"
                     /db_xref="HGNC:HGNC:30376"
                     /db_xref="MIM:607290"
                     /translation="MASRGLSLFPESCPDFCCGTCDDQYCCSDVLKKFVWSEERCAVP
                     EASVPASVEPVEQLGSALRFRPGYNDPMSGFGATLAVGLTIFVLSVVTIIICFTCSCC
                     CLYKTCRRPRPVVTTTTSTTVVHAPYPQPPSVPPSYPGPSYQGYHTMPPQPGMPAAPY
                     PMQYPPPYPAQPMGPPAYHETLAGGAAAPYPASQPPYNPAYMDAPKAAL"
     exon            546..626
                     /gene="SHISA5"
                     /gene_synonym="SCOTIN"
                     /inference="alignment:Splign:2.1.0"
     exon            627..742
                     /gene="SHISA5"
                     /gene_synonym="SCOTIN"
                     /inference="alignment:Splign:2.1.0"
     exon            743..955
                     /gene="SHISA5"
                     /gene_synonym="SCOTIN"
                     /inference="alignment:Splign:2.1.0"
     exon            956..2266
                     /gene="SHISA5"
                     /gene_synonym="SCOTIN"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2247..2252
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SHISA5"
                     /gene_synonym="SCOTIN"
                     /note="hexamer: AATAAA"
     polyA_site      2266
                     /gene="SHISA5"
                     /gene_synonym="SCOTIN"
                     /note="major polyA site"
ORIGIN      
        1 gccagtttct agctcagtcc tggcccactg cgccagcgct gagcctgcca gggctggggc
       61 tggggatcac cttgggatga tggtgtcagt cccagggggc aggagatcga gtgtcctctg
      121 agctggcgac tgggcctgta gaagggaacc ggcatttgtg gagtgtctac tgagtgccaa
      181 ggtctgcgct gggcactgtc ctcgcaccgc ctcacctagt cctcacgtag ccctcgggca
      241 agtgaggatc cgccgggact gcggctggga gggatggctg tggctgtccc ccagcccaca
      301 cagtaggcgc tcagtgtcag ggtgcatatt cccggggacg ccctccaggc ctgagagctg
      361 ggggccgccg ccgcccccca tgcatccgca cgtggtgagg tgtgtatggc ttcccgtgga
      421 ctcagcctct tccccgagtc ctgtccagat ttctgctgtg gtacctgtga tgaccaatac
      481 tgctgctctg acgtgctgaa gaaatttgtg tggagcgagg aaaggtgtgc tgtgcctgag
      541 gccagcgtgc ctgccagtgt agagccggtg gagcagctgg gctcggcgct gaggtttcgc
      601 cctggctaca acgaccccat gtcagggttc ggagcgacct tggccgttgg cctgaccatc
      661 tttgtgctgt ctgtcgtcac tatcatcatc tgcttcacct gctcctgctg ctgcctttac
      721 aagacgtgcc gccgaccacg tccggttgtc accaccacca catccaccac tgtggtgcat
      781 gccccttatc ctcagcctcc aagtgtgccg cccagctacc ctggaccaag ctaccagggc
      841 taccacacca tgccgcctca gccagggatg ccagcagcac cctacccaat gcagtaccca
      901 ccaccttacc cagcccagcc catgggccca ccggcctacc acgagaccct ggctggagga
      961 gcagccgcgc cctaccccgc cagccagcct ccttacaacc cggcctacat ggatgccccg
     1021 aaggcggccc tctgagcatt ccctggcctc tctggctgcc acttggttat gttgtgtgtg
     1081 tgcgtgagtg gtgtgcaggc gcggttcctt acgccccatg tgtgctgtgt gtgtccaggc
     1141 acggttcctt acgccccatg tgtgctgtgt gtgtcctgcc tgtatatgtg gcttcctctg
     1201 atgctgacaa ggtggggaac aatccttgcc agagtgggct gggaccagac tttgttctct
     1261 tcctcacctg aaattatgct tcctaaaatc tcaagccaaa ctcaaagaat ggggtggtgg
     1321 ggggcaccct gtgaggtggc ccctgagagg tgggggcctc tccagggcac atctggagtt
     1381 cttctccagc ttaccctagg gtgaccaagt agggcctgtc acaccagggt ggcgcagctt
     1441 tctgtgtgat gcagatgtgt cctggtttcg gcagcgtagc cagctgctgc ttgaggccat
     1501 ggctcgtccc cggagttggg ggtacccgtt gcagagccag ggacatgatg caggcgaagc
     1561 ttgggatctg gccaagttgg actttgatcc tttgggcaga tgtcccattg ctccctggag
     1621 cctgtcatgc ctgttgggga tcaggcagcc tcctgatgcc agaacacctc aggcagagcc
     1681 ctactcagct gtacctgtct gcctggactg tcccctgtcc ccgcatctcc cctgggacca
     1741 gctggagggc cacatgcaca cacagcctag ctgcccccag ggagctctgc tgcccttgct
     1801 ggccctgccc ttcccacagg tgagcagggc tcctgtccac cagcacactc agttctcttc
     1861 cctgcagtgt tttcatttta ttttagccaa acattttgcc tgttttctgt ttcaaacatg
     1921 atagttgata tgagactgaa acccctgggt tgtggaggga aattggctca gagatggaca
     1981 acctggcaac tgtgagtccc tgcttcccga caccagcctc atggaatatg caacaactcc
     2041 tgtaccccag tccacggtgt tctggcagca gggacacctg ggccaatggg ccatctggac
     2101 caaaggtggg gtgtggggcc ctggatggca gctctggccc agacatgaat acctcgtgtt
     2161 cctcctccct ctattactgt ttcaccagag ctgtcttagc tcaaatctgt tgtgtttctg
     2221 agtctagggt ctgtacactt gtttataata aatgcaatcg tttgga
//
