LOCUS       NM_018381               1928 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens shiftless antiviral inhibitor of ribosomal
            frameshifting (SHFL), transcript variant 1, mRNA.
ACCESSION   NM_018381
VERSION     NM_018381.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Zhang,Y., Feng,Y., Liu,Y., Liu,L., Xia,X. and Zhang,A.M.
  TITLE     Genetic polymorphisms in the C19orf66 gene influenced HIV-1
            infection in a Yunnan population
  JOURNAL   PeerJ 11, e16005 (2023)
   PUBMED   37701839
  REMARK    GeneRIF: Genetic polymorphisms in the C19orf66 gene influenced
            HIV-1 infection in a Yunnan population.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Liu,N., Liu,M., Yang,J., Dong,S., Yue,M., Huang,P., Xia,X. and
            Zhang,A.M.
  TITLE     Association of genetic polymorphisms in the C19orf66 gene and
            biochemical indices of HBV infected individuals in Yunnan
  JOURNAL   Front Cell Infect Microbiol 13, 1180366 (2023)
   PUBMED   37293200
  REMARK    GeneRIF: Association of genetic polymorphisms in the C19orf66 gene
            and biochemical indices of HBV infected individuals in Yunnan.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1928)
  AUTHORS   Rodriguez,W., Mehrmann,T., Hatfield,D. and Muller,M.
  TITLE     Shiftless Restricts Viral Gene Expression and Influences RNA
            Granule Formation during Kaposi's Sarcoma-Associated Herpesvirus
            Lytic Replication
  JOURNAL   J Virol 96 (22), e0146922 (2022)
   PUBMED   36326276
  REMARK    GeneRIF: Shiftless Restricts Viral Gene Expression and Influences
            RNA Granule Formation during Kaposi's Sarcoma-Associated
            Herpesvirus Lytic Replication.
REFERENCE   4  (bases 1 to 1928)
  AUTHORS   Hanners,N.W., Mar,K.B., Boys,I.N., Eitson,J.L., De La
            Cruz-Rivera,P.C., Richardson,R.B., Fan,W., Wight-Carter,M. and
            Schoggins,J.W.
  TITLE     Shiftless inhibits flavivirus replication in vitro and is
            neuroprotective in a mouse model of Zika virus pathogenesis
  JOURNAL   Proc Natl Acad Sci U S A 118 (49) (2021)
   PUBMED   34873063
REFERENCE   5  (bases 1 to 1928)
  AUTHORS   Yu,D., Zhao,Y., Pan,J., Yang,X., Liang,Z., Xie,S. and Cao,R.
  TITLE     C19orf66 Inhibits Japanese Encephalitis Virus Replication by
            Targeting -1 PRF and the NS3 Protein
  JOURNAL   Virol Sin 36 (6), 1443-1455 (2021)
   PUBMED   34309824
  REMARK    GeneRIF: C19orf66 Inhibits Japanese Encephalitis Virus Replication
            by Targeting -1 PRF and the NS3 Protein.
REFERENCE   6  (bases 1 to 1928)
  AUTHORS   Wang,X., Xuan,Y., Han,Y., Ding,X., Ye,K., Yang,F., Gao,P.,
            Goff,S.P. and Gao,G.
  TITLE     Regulation of HIV-1 Gag-Pol Expression by Shiftless, an Inhibitor
            of Programmed -1 Ribosomal Frameshifting
  JOURNAL   Cell 176 (3), 625-635 (2019)
   PUBMED   30682371
REFERENCE   7  (bases 1 to 1928)
  AUTHORS   Balinsky,C.A., Schmeisser,H., Wells,A.I., Ganesan,S., Jin,T.,
            Singh,K. and Zoon,K.C.
  TITLE     IRAV (FLJ11286), an Interferon-Stimulated Gene with Antiviral
            Activity against Dengue Virus, Interacts with MOV10
  JOURNAL   J Virol 91 (5), e01606-16 (2017)
   PUBMED   27974568
  REMARK    GeneRIF: IRAV is an RNA binding protein and localizes to
            cytoplasmic processing bodies (P bodies) in uninfected cells, where
            it interacts with the MOV10 RISC complex RNA helicase, suggesting a
            role for IRAV in the processing of viral RNA.
            Publication Status: Online-Only
REFERENCE   8  (bases 1 to 1928)
  AUTHORS   Suzuki,Y., Chin,W.X., Han,Q., Ichiyama,K., Lee,C.H., Eyo,Z.W.,
            Ebina,H., Takahashi,H., Takahashi,C., Tan,B.H., Hishiki,T.,
            Ohba,K., Matsuyama,T., Koyanagi,Y., Tan,Y.J., Sawasaki,T.,
            Chu,J.J., Vasudevan,S.G., Sano,K. and Yamamoto,N.
  TITLE     Characterization of RyDEN (C19orf66) as an Interferon-Stimulated
            Cellular Inhibitor against Dengue Virus Replication
  JOURNAL   PLoS Pathog 12 (1), e1005357 (2016)
   PUBMED   26735137
  REMARK    GeneRIF: we identified a previously uncharacterized gene, C19orf66,
            as an IFN-stimulated gene (ISG) that inhibits Dengue virus
            replication
            Publication Status: Online-Only
REFERENCE   9  (bases 1 to 1928)
  AUTHORS   Grossmann,A., Benlasfer,N., Birth,P., Hegele,A., Wachsmuth,F.,
            Apelt,L. and Stelzl,U.
  TITLE     Phospho-tyrosine dependent protein-protein interaction network
  JOURNAL   Mol Syst Biol 11 (3), 794 (2015)
   PUBMED   25814554
  REMARK    Publication Status: Online-Only
REFERENCE   10 (bases 1 to 1928)
  AUTHORS   Lee,O.H., Kim,H., He,Q., Baek,H.J., Yang,D., Chen,L.Y., Liang,J.,
            Chae,H.K., Safari,A., Liu,D. and Songyang,Z.
  TITLE     Genome-wide YFP fluorescence complementation screen identifies new
            regulators for telomere signaling in human cells
  JOURNAL   Mol Cell Proteomics 10 (2), M110.001628 (2011)
   PUBMED   21044950
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DB481173.1, AK002148.1 and BC010847.1.
            
            On Nov 23, 2018 this sequence version replaced NM_018381.3.
            
            Summary: This gene is an interferon stimulated gene (ISG) that
            inhibits viral replication. The encoded protein binds nucleic acids
            and inhibits programmed -1 ribosomal frameshifting required for
            translation by many RNA viruses. Viruses inhibited by the protein
            include Zika virus, dengue virus and the coronaviruses, SARS-CoV
            and SARS-CoV2. [provided by RefSeq, Aug 2021].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.1158600.1,
                                           SRR3476690.364349.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            coronavirus related    :: involved in immune response or antiviral
                                      activity
            MANE Ensembl match     :: ENST00000253110.16/ ENSP00000253110.10
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-16                DB481173.1         188-203
            17-681              AK002148.1         1-665
            682-1928            BC010847.1         784-2030
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19p13.2"
     gene            1..1928
                     /gene="SHFL"
                     /gene_synonym="C19orf66; IRAV; RyDEN; SFL"
                     /note="shiftless antiviral inhibitor of ribosomal
                     frameshifting"
                     /db_xref="GeneID:55337"
                     /db_xref="HGNC:HGNC:25649"
                     /db_xref="MIM:616808"
     exon            1..132
                     /gene="SHFL"
                     /gene_synonym="C19orf66; IRAV; RyDEN; SFL"
                     /inference="alignment:Splign:2.1.0"
     CDS             112..987
                     /gene="SHFL"
                     /gene_synonym="C19orf66; IRAV; RyDEN; SFL"
                     /note="isoform 1 is encoded by transcript variant 1;
                     UPF0515 protein C19orf66; repressor of yield of DENV
                     protein; Repressor of yield of Dengue virus; shiftless;
                     interferon-regulated antiviral protein; shiftless
                     antiviral inhibitor of ribosomal frameshifting protein"
                     /codon_start=1
                     /product="shiftless antiviral inhibitor of ribosomal
                     frameshifting protein isoform 1"
                     /protein_id="NP_060851.2"
                     /db_xref="CCDS:CCDS45957.1"
                     /db_xref="GeneID:55337"
                     /db_xref="HGNC:HGNC:25649"
                     /db_xref="MIM:616808"
                     /translation="MSQEGVELEKSVRRLREKFHGKVSSKKAGALMRKFGSDHTGVGR
                     SIVYGVKQKDGQELSNDLDAQDPPEDMKQDRDIQAVATSLLPLTEANLRMFQRAQDDL
                     IPAVDRQFACSSCDHVWWRRVPQRKEVSRCRKCRKRYEPVPADKMWGLAEFHCPKCRH
                     NFRGWAQMGSPSPCYGCGFPVYPTRILPPRWDRDPDRRSTHTHSCSAADCYNRREPHV
                     PGTSCAHPKSRKQNHLPKVLHPSNPHISSGSTVATCLSQGGLLEDLDNLILEDLKEEE
                     EEEEEVEDEEGGPRE"
     exon            133..256
                     /gene="SHFL"
                     /gene_synonym="C19orf66; IRAV; RyDEN; SFL"
                     /inference="alignment:Splign:2.1.0"
     exon            257..306
                     /gene="SHFL"
                     /gene_synonym="C19orf66; IRAV; RyDEN; SFL"
                     /inference="alignment:Splign:2.1.0"
     exon            307..345
                     /gene="SHFL"
                     /gene_synonym="C19orf66; IRAV; RyDEN; SFL"
                     /inference="alignment:Splign:2.1.0"
     exon            346..495
                     /gene="SHFL"
                     /gene_synonym="C19orf66; IRAV; RyDEN; SFL"
                     /inference="alignment:Splign:2.1.0"
     exon            496..599
                     /gene="SHFL"
                     /gene_synonym="C19orf66; IRAV; RyDEN; SFL"
                     /inference="alignment:Splign:2.1.0"
     exon            600..754
                     /gene="SHFL"
                     /gene_synonym="C19orf66; IRAV; RyDEN; SFL"
                     /inference="alignment:Splign:2.1.0"
     exon            755..1928
                     /gene="SHFL"
                     /gene_synonym="C19orf66; IRAV; RyDEN; SFL"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1907..1912
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SHFL"
                     /gene_synonym="C19orf66; IRAV; RyDEN; SFL"
                     /note="hexamer: AATAAA"
     polyA_site      1928
                     /gene="SHFL"
                     /gene_synonym="C19orf66; IRAV; RyDEN; SFL"
                     /note="major polyA site"
ORIGIN      
        1 gaggcaccgc cccctgccct gcgcggctgc tggaccgacg ggcgcaccca ggtagggggg
       61 cggctgagcc gcgcagtgcg gaccctcgcg gggaactgcg ccgccgccac catgtctcag
      121 gaaggtgtgg agctggagaa gagcgtccgg cgcctccggg agaagtttca tgggaaggta
      181 tcctccaaga aggcgggggc tctgatgagg aaattcggca gcgaccacac gggagtgggg
      241 cgctccatcg tgtacggggt aaagcaaaaa gatggccaag aactaagtaa cgatctggat
      301 gcccaggatc caccagaaga tatgaagcag gaccgggaca ttcaggcagt ggcgacctcc
      361 ctcctgccac tgacagaagc caacctacgc atgtttcaac gtgcccagga cgaccttatc
      421 cctgctgtgg accggcagtt tgcctgctcc tcctgcgacc acgtctggtg gcgccgcgtg
      481 ccccagcgga aggaggtatc ccggtgccgg aaatgccgga agcgctacga gccagtgcca
      541 gctgacaaga tgtggggcct ggctgagttc cactgcccga agtgtcggca caacttccgg
      601 ggctgggcac agatggggtc cccgtccccc tgctacgggt gcggcttccc cgtgtatcca
      661 acacggatcc tccccccgcg ctgggaccgg gacccggatc gccgcagcac ccacactcac
      721 tcctgctcag ctgccgactg ctacaaccgg cgagagcccc acgtgcctgg gacatcctgt
      781 gctcacccca agagccggaa gcagaaccac ctgcccaaag tgctccaccc cagcaaccct
      841 cacattagca gtggctccac tgtggccacc tgcttgagcc agggtggcct cctggaagac
      901 ctggacaacc tcatcctgga ggacctgaag gaggaggagg aggaagagga ggaggtggag
      961 gacgaggagg gcgggcccag ggagtgaccc ctgccaggtg cagatacaaa ccagacacgg
     1021 tctgtggcta ctttgtgtta ttataagata tgagctcaaa ccgagatatg aatgaccttg
     1081 gggagccatc tgaggccaag atattgacgg gggggattcc tgggtcccat tttcagcgcc
     1141 cagggtcaca gatccacagt gggaagttct gtgggacaca ttggcactga gccacaaaga
     1201 aggtgtggcc agaacaactt gggctcctgc tgaccaatgt cctctagggc ctaggggaca
     1261 gaggaacaca gagtcacagc ttcaggggcc gaatgagcat ggcggccttc ctgagagaat
     1321 atgccccacc acgaaactca gcccagtaga caccatcctg gtagcggctt cggtagtggc
     1381 cgccgtggtg ccacacaccg ttgaggttgg agtgggcaca ggcatggtac caccagcctc
     1441 cccgctggta cagggcacag ttacctgagg ggagagagag agtccatgtc ctctcaccag
     1501 aataaaagcc tctacctgca cctcacagtg caaggctttt gccaggcatc ccctggcccc
     1561 tcccattctt attgaataca agccctgatc ttccatctcc tcagcaaaaa aataggagcc
     1621 ctggcccccc aactttcttc agagtaatag ccttaattcc ttccctatct ccttaccaaa
     1681 gtacaagtca catctttccc accttttctg caaactagga gtctaccgtt cattccttta
     1741 tcaaagaaaa gtatctactt cctttctaga ataagagtac tagctctcac cctctgccct
     1801 ttacttgaac aggagtcttg attctttttt tgcctcatca gagaaggaat ctggactccc
     1861 catcccccca ccaggataaa agtcctgacc tttgttctct tgacggaata aaagcttgct
     1921 tatcctta
//
