LOCUS       NM_003028               6035 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens SH2 domain containing adaptor protein B (SHB), mRNA.
ACCESSION   NM_003028
VERSION     NM_003028.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6035)
  AUTHORS   Wagner,M.J., Hsiung,M.S., Gish,G.D., Bagshaw,R.D., Doodnauth,S.A.,
            Soliman,M.A., Jorgensen,C., Tucholska,M. and Rottapel,R.
  TITLE     The Shb scaffold binds the Nck adaptor protein, p120 RasGAP, and
            Chimaerins and thereby facilitates heterotypic cell segregation by
            the receptor EphB2
  JOURNAL   J Biol Chem 295 (12), 3932-3944 (2020)
   PUBMED   32060095
  REMARK    GeneRIF: The Shb scaffold binds the Nck adaptor protein, p120
            RasGAP, and Chimaerins and thereby facilitates heterotypic cell
            segregation by the receptor EphB2.
REFERENCE   2  (bases 1 to 6035)
  AUTHORS   Vanli,G., Cuesta-Marban,A. and Widmann,C.
  TITLE     Evaluation and validation of commercial antibodies for the
            detection of Shb
  JOURNAL   PLoS One 12 (12), e0188311 (2017)
   PUBMED   29194461
  REMARK    GeneRIF: This article provides a resource for the available
            molecular tools that can be used in future research on Shb
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6035)
  AUTHORS   Jamalpour,M., Li,X., Cavelier,L., Gustafsson,K., Mostoslavsky,G.,
            Hoglund,M. and Welsh,M.
  TITLE     Tumor SHB gene expression affects disease characteristics in human
            acute myeloid leukemia
  JOURNAL   Tumour Biol 39 (10), 1010428317720643 (2017)
   PUBMED   28982308
  REMARK    GeneRIF: It is concluded that tumor SHB gene expression relates to
            acute myeloid leukemia survival
REFERENCE   4  (bases 1 to 6035)
  AUTHORS   Welsh,M., Jamalpour,M., Zang,G. and Akerblom,B.
  TITLE     The role of the Src Homology-2 domain containing protein B (SHB) in
            beta cells
  JOURNAL   J Mol Endocrinol 56 (1), R21-R31 (2016)
   PUBMED   26489764
  REMARK    GeneRIF: SHB plays a role in integrating adaptive responses to
            various stimuli by simultaneously modulating cellular responses in
            different cell-types. [review]
            Review article
REFERENCE   5  (bases 1 to 6035)
  AUTHORS   Leung,K.K., Hause,R.J. Jr., Barkinge,J.L., Ciaccio,M.F., Chuu,C.P.
            and Jones,R.B.
  TITLE     Enhanced prediction of Src homology 2 (SH2) domain binding
            potentials using a fluorescence polarization-derived c-Met, c-Kit,
            ErbB, and androgen receptor interactome
  JOURNAL   Mol Cell Proteomics 13 (7), 1705-1723 (2014)
   PUBMED   24728074
REFERENCE   6  (bases 1 to 6035)
  AUTHORS   Welsh,M., Songyang,Z., Frantz,J.D., Trub,T., Reedquist,K.A.,
            Karlsson,T., Miyazaki,M., Cantley,L.C., Band,H. and Shoelson,S.E.
  TITLE     Stimulation through the T cell receptor leads to interactions
            between SHB and several signaling proteins
  JOURNAL   Oncogene 16 (7), 891-901 (1998)
   PUBMED   9484780
REFERENCE   7  (bases 1 to 6035)
  AUTHORS   Karlsson,T. and Welsh,M.
  TITLE     Apoptosis of NIH3T3 cells overexpressing the Src homology 2 domain
            protein Shb
  JOURNAL   Oncogene 13 (5), 955-961 (1996)
   PUBMED   8806685
REFERENCE   8  (bases 1 to 6035)
  AUTHORS   Karlsson,T., Songyang,Z., Landgren,E., Lavergne,C., Di Fiore,P.P.,
            Anafi,M., Pawson,T., Cantley,L.C., Claesson-Welsh,L. and Welsh,M.
  TITLE     Molecular interactions of the Src homology 2 domain protein Shb
            with phosphotyrosine residues, tyrosine kinase receptors and Src
            homology 3 domain proteins
  JOURNAL   Oncogene 10 (8), 1475-1483 (1995)
   PUBMED   7537362
REFERENCE   9  (bases 1 to 6035)
  AUTHORS   Yulug,I.G., Hillermann,R. and Fisher,E.M.
  TITLE     The SHB adaptor protein maps to human chromosome 9
  JOURNAL   Genomics 24 (3), 615-617 (1994)
   PUBMED   7713524
REFERENCE   10 (bases 1 to 6035)
  AUTHORS   Welsh,M., Mares,J., Karlsson,T., Lavergne,C., Breant,B. and
            Claesson-Welsh,L.
  TITLE     Shb is a ubiquitously expressed Src homology 2 protein
  JOURNAL   Oncogene 9 (1), 19-27 (1994)
   PUBMED   8302579
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL161448.16, AL583849.10 and AL138752.5.
            
            On Nov 23, 2018 this sequence version replaced NM_003028.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: X75342.1, BC136581.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000377707.4/ ENSP00000366936.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-14                AL161448.16        79853-79866         c
            15-1299             AL161448.16        78568-79852         c
            1300-1420           AL161448.16        26650-26770         c
            1421-1636           AL583849.10        31434-31649         c
            1637-1808           AL583849.10        12695-12866         c
            1809-1928           AL583849.10        5447-5566           c
            1929-6035           AL138752.5         25185-29291
FEATURES             Location/Qualifiers
     source          1..6035
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9p13.1"
     gene            1..6035
                     /gene="SHB"
                     /gene_synonym="bA3J10.2"
                     /note="SH2 domain containing adaptor protein B"
                     /db_xref="GeneID:6461"
                     /db_xref="HGNC:HGNC:10838"
                     /db_xref="MIM:600314"
     exon            1..1299
                     /gene="SHB"
                     /gene_synonym="bA3J10.2"
                     /inference="alignment:Splign:2.1.0"
     CDS             583..2112
                     /gene="SHB"
                     /gene_synonym="bA3J10.2"
                     /note="SHB adaptor protein (a Src homology 2 protein); SHB
                     (Src homology 2 domain containing) adaptor protein B; Src
                     homology 2 domain containing adaptor protein B"
                     /codon_start=1
                     /product="SH2 domain-containing adapter protein B"
                     /protein_id="NP_003019.2"
                     /db_xref="CCDS:CCDS43806.1"
                     /db_xref="GeneID:6461"
                     /db_xref="HGNC:HGNC:10838"
                     /db_xref="MIM:600314"
                     /translation="MAKWLNKYFSLGNSKTKSPPQPPRPDYREQRRRGERPSQPPQAV
                     PQASSAASASCGPATASCFSASSGSLPDDSGSTSDLIRAYRAQKERDFEDPYNGPGSS
                     LRKLRAMCRLDYCGGSGEPGGVQRAFSASSASGAAGCCCASSGAGAAASSSSSSGSPH
                     LYRSSSERRPATPAEVRYISPKHRLIKVESAAGGGAGDPLGGACAGGRTWSPTACGGK
                     KLLNKCAASAAEESGAGKKDKVTIADDYSDPFDAKNDLKSKAGKGESAGYMEPYEAQR
                     IMTEFQRQESVRSQHKGIQLYDTPYEPEGQSVDSDSESTVSPRLRESKLPQDDDRPAD
                     EYDQPWEWNRVTIPALAAQFNGNEKRQSSPSPSRDRRRQLRAPGGGFKPIKHGSPEFC
                     GILGERVDPAVPLEKQIWYHGAISRGDAENLLRLCKECSYLVRNSQTSKHDYSLSLRS
                     NQGFMHMKLAKTKEKYVLGQNSPPFDSVPEVIHYYTTRKLPIKGAEHLSLLYPVAVRT
                     L"
     exon            1300..1420
                     /gene="SHB"
                     /gene_synonym="bA3J10.2"
                     /inference="alignment:Splign:2.1.0"
     exon            1421..1636
                     /gene="SHB"
                     /gene_synonym="bA3J10.2"
                     /inference="alignment:Splign:2.1.0"
     exon            1637..1808
                     /gene="SHB"
                     /gene_synonym="bA3J10.2"
                     /inference="alignment:Splign:2.1.0"
     exon            1809..1928
                     /gene="SHB"
                     /gene_synonym="bA3J10.2"
                     /inference="alignment:Splign:2.1.0"
     exon            1929..6035
                     /gene="SHB"
                     /gene_synonym="bA3J10.2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2361..2366
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SHB"
                     /gene_synonym="bA3J10.2"
                     /note="hexamer: ATTAAA"
     polyA_site      2383
                     /gene="SHB"
                     /gene_synonym="bA3J10.2"
                     /note="major polyA site"
     regulatory      2780..2785
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SHB"
                     /gene_synonym="bA3J10.2"
                     /note="hexamer: AATAAA"
     polyA_site      2801
                     /gene="SHB"
                     /gene_synonym="bA3J10.2"
                     /note="major polyA site"
     regulatory      6012..6017
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SHB"
                     /gene_synonym="bA3J10.2"
                     /note="hexamer: AATATA"
     polyA_site      6035
                     /gene="SHB"
                     /gene_synonym="bA3J10.2"
ORIGIN      
        1 agtggcagcg acaggcgccg ccgggacggg cacgggcgcg cgggctccgg cgggcgccgg
       61 ctgccttcct ccgtcgctcg ctgtctctcc cggccgcatt ctcctccgct gcggggccga
      121 gctctcccca gcgctcgcag gaaggaagaa gggagccgag gacgccgaga agttcccgcg
      181 gcagccgcgg atcccggcca aggcggaggc tgcggctccg acggggcagg agcgcgatcc
      241 acggcgaggg gcgtacggcc aaagggtccg cggcgtggag cgctcggacc ttccgctctc
      301 ccccgggcgt gggccgggac cccatgagac gcgcccacga ggggcgcgag attcctagct
      361 tgggcggcgc taggcggagg gaggtgttgc agccgccgga gccagagagc tgccggcagg
      421 aggcggcggc ggcaagaact tgaacttggc gtcgggagcg ggcgccccgg acgccccccg
      481 ccggggccgg ggcgccgagg gacctgcgcc gcagcgctgc cccccgaatg gccgcggcgg
      541 cggaccgggc tcccgcgccg cggccctagg cggcctctcg ccatggccaa gtggctaaac
      601 aagtacttca gcttgggcaa cagcaagacc aagagccccc cgcagccgcc gcggccagac
      661 taccgcgagc agcggcgccg aggcgagcgg ccttcgcagc ccccccaggc cgtgccgcag
      721 gcctcctccg ccgcctcggc gtcctgcggt ccggccaccg cctcctgctt ctcagcctct
      781 tcgggctcgc tgcccgacga cagcggcagc accagcgacc tcatccgcgc ctaccgcgcg
      841 cagaaggagc gagacttcga ggacccctac aacgggcctg gctcgtcgct gcgcaaactg
      901 cgcgccatgt gccgcctgga ctactgcggc ggcagcgggg agccaggcgg ggtccagcgc
      961 gccttctcgg cctcgtccgc gtcgggcgcc gcgggctgtt gctgcgcctc ctcgggcgcg
     1021 ggggccgccg cgtcctcgtc ctcgtcctcc ggctctccgc atctctaccg cagcagcagc
     1081 gagcggcggc ccgccacgcc ggccgaggtg cgctacatct cccccaagca ccgcctcatc
     1141 aaagtggaga gcgccgcggg cggtggggcc ggggaccccc tggggggcgc ctgcgcgggc
     1201 ggccgcacct ggagcccgac ggcctgcgga ggcaagaaac tgctcaacaa gtgcgccgcc
     1261 tcagccgcgg aggagagcgg ggccggcaag aaggacaagg tgaccatagc cgatgactac
     1321 tcagatccct ttgatgccaa gaatgatctc aagagcaaag caggaaaggg ggagagtgct
     1381 ggctacatgg agccctatga ggcacagagg atcatgacag aatttcagag gcaggaaagt
     1441 gtccggtccc agcataaagg tatccagtta tatgacaccc cttacgaacc tgaaggccaa
     1501 agtgttgact cagactcgga gagcacagtc agcccccgac tgcgggagag caagctgccc
     1561 caggatgacg acaggcccgc cgatgagtac gaccagcctt gggagtggaa ccgggtcacc
     1621 atcccagccc tggcagcaca gtttaatggc aacgagaagc ggcagtcatc cccctcacct
     1681 tcgcgggacc ggcggcgcca gcttcgtgcc cctggagggg gctttaagcc tatcaaacat
     1741 gggagccctg agttctgcgg gatcctagga gaaagggtgg atcctgccgt ccccctggag
     1801 aagcaaatat ggtatcacgg agccatcagc agaggagacg ccgagaacct gctgcgactc
     1861 tgcaaggagt gtagctacct tgtccggaac agccagacca gcaagcatga ctactccctc
     1921 tccctgagga gcaaccaggg ttttatgcac atgaaactgg ccaaaaccaa agagaaatac
     1981 gttctgggtc agaacagccc tccgttcgac agtgtcccgg aagtcatcca ctactacacc
     2041 accagaaagc tacccatcaa aggggctgag cacttgtccc tcctctatcc cgtggctgtg
     2101 aggaccctgt gagcggacca gacctgccct gctctgtgac agagcctgag acttggaggt
     2161 gccagaggcc ccccaccaac cagcccagcc actgttgctg gctgtgtcgt ttgtgttgtg
     2221 tgtatggtac tagcacacca ctgcatgtct ctagaatgct gttgccactt acgggggctg
     2281 gagaaggcct ggataaagac agaagggcgg caacacacca ccccagcccc cacccccacc
     2341 ctctccttga gtttctgtga attaaaatat ttgcaaatcc aaagagatgc cttccaggat
     2401 gaacaaaggc agaccagctc cgaggggcgg ggtagggtgg ggcgggagct ccctggctga
     2461 catctctttg ttctccagct gtctcaaatt cacacctctg tacagggcaa agttttccca
     2521 caggagcccc cctactgatg ctgggagcca aatgctggtg ctttgagagt cccggaggcc
     2581 ccggggttcc cgccccgctg gtgtgtatat gtgtgtctgt gtgagtgtgt gtgtgagtac
     2641 agatgtgaga aggtggtcac acacagatgg gtaagcccac tgatctactt gtagtcactc
     2701 agtgtaatca ttaggctatc ttcaaggaat cattgtgcag tcaaaaagag gattcaatta
     2761 tttatgaata gggtgtgtaa ataaaatagt catttaatat atagccttat ccttttttga
     2821 tatactgcag agggtctctg agggggcagt tctggtttca ctcccagagc aaggcgtgtc
     2881 tcctctgggc ttgctgctgt ggcaggcccc agggagggga gggaggccga ggcctcagct
     2941 ccagccctca gcagctctgg acacaagagc cgtctgctct gacaactggg ctgaggcagt
     3001 cggtagcact ctgggagtca acccagagct cagagttcag gcttcagcag tggacatagt
     3061 caggagcctc cagtgttgac caaaaaacca gtctttccct cctgcagtgg gacgtctgtg
     3121 ttgacacccc cccaacccct ggacacagca cccagctggc tacaaagggc tctctcccca
     3181 ctatctgtta gcaccacctg ggaggtattt ggcatgtagg gaaagggaga cagagaagga
     3241 acgtcatttg cccaaagcca cacagctcac cagcagcaga gcggttctgc agccaatgct
     3301 ctttcgttgg ttctgcatgg ccttgctgtc accctgccca tggaggaggg agaatcctga
     3361 cctcccaggg acatagagcc ctcagtggtc cacactgctt ccacacacaa gaacacctac
     3421 acacacacac acacacacac acacagccct cagtggtcca cactgcttcc acacacaaga
     3481 acacctacac acacacacac acacacacac agccctcagt ggtccacact gcttccacac
     3541 acaagaacac ctacacacgc acacgcacac gcgacctctc tcttgcttgc catagcaggg
     3601 tcctcccaca caggccaggc agccccgatg aggctaagag tttctcagct ctcctgcttt
     3661 ttccacccac ccagtagcat gaagatgtgg ctgccctctc ctgaatgaga tacaagggtt
     3721 ccagagcctt cgccaggcac cagctggtct gaatgaacta gaagtttgac gactgtcatc
     3781 cctggcccac ctgggcagag cacaaggggc cgagtttgaa tgtcatcagc aggttcagac
     3841 atcagcgctg ctcatgtctt tagagactgt ttggaggtgg ttccagctca ctgttcacat
     3901 gcagagacac aggcccggag aaggaggact cgccaaggcc acgtgtcgag ctgggactga
     3961 gccctgcccc tttaggtagc tgcgtggact tcagcggttt gtgcaaagcc ctgcccttgg
     4021 tgacccgcct ttagtgacca gtctgcctct tgggtcttcc tcccaggcct tgtctggcac
     4081 gtccacgtgg cccagccctc atagagacca cgctggctgt ggcctgggtt taatctctta
     4141 aagaagccaa tcctttcctt gcctgtttgg catttcctaa aacatccctc aatgagggag
     4201 gaaggcaggg acaccctgga acgattcgtg ctttcctttg ggggagtggg aaggtaggtg
     4261 ggaaatgaca agatgagtga ggagttggca cttggccaaa cagaggagcc tggaccccaa
     4321 ggcttattct cagaggaggc ccaagagagg ctgaggaaga gggggaacaa ccctccggcg
     4381 ggggaggagg gtgagacctc acactggcaa gttcaggtcc agccacttcc tgaggctgct
     4441 gggtggggcc gcacctcacg gtccttccct gtagaccatc ttcccgggca cattgctcct
     4501 cagaaaaggc tctgggaggg tctcagtgga agctggcagc tgtgccctct ggccctgact
     4561 tggcctctta gggtattcct gggaccctcc ctctgtccct ggccttcgtc ttcctgaagt
     4621 ttccctggga ggcccaacct cctgcccctc tcagggacag aggccagcag aggagccagc
     4681 ttacaggatg ggagctgctg aaaagaattt ttttccctta attttatgaa cctctccctc
     4741 ttcttcctct aattgtgacc gtttcctgtg tcagcctgta ttcatgtttt cttggacatc
     4801 taacaattga aacagctttt ccctttcaga aacactcaca ttcaggttac tggctttggt
     4861 ttggtttttg ttttgggttt gttttttttt tttttcatct gctgccaatt aattggggaa
     4921 aaaattgagc tggatttctt tgagagaaaa gaaaagctcc tgccacacaa cgtcgcgccc
     4981 tcggcatttt gtctgagaac ctccagcact agcggctgtt tctgtgtctc ccttctttgc
     5041 tggctttatt attgaccata atgcctgccc acttgagcaa ctctgtcctt gagcgctgag
     5101 gagtcaaggg cccttctggc agccccccaa acaggatggc gcccacagaa agaaaggagg
     5161 ctttgtaggg gaagagggcc ccaccgcctg ccagtcccgt cctgaagtca gaagaatggc
     5221 ccaggctgac accctctcct ggagagaggc tcacagcgta gctgttccca ggaaaggggt
     5281 gcaaagactc cgaggcttca ggaggcaggc cctgtcctca cagggcggct gtgggccggg
     5341 gcccctctgt ggcctgagag tgagcaggag ggaaacctga agcagctacc cgcaaggcgt
     5401 ccggtcaaca gggtggagct gcaggctccc tctgcccatt cccagaacca gcggccccct
     5461 ttgtaagatg ctgaacacga agttccggta gctccccaga cgctgtctca gaagtggtga
     5521 tatcattgac caagggcctg gcaaggaccg gcagccaggg tccccatttg ctcttgcagc
     5581 cctagggact gtgcaggtag aagccctgcc agcagagcag gctggcgttt ggtgttggcc
     5641 cacagacacc cgtgaagggt catggccgaa tcaggcagct gtccagccaa ctgtctctcc
     5701 acccttccag aattggtgat cagcgaagac caaattcggc aagccatcca gagggagtgc
     5761 agggcgcaca gtcttagatg tgggcgcacc tcccagaacc ccacggggac ttgttcacag
     5821 gcgcgcttgc aggagggcaa gccccttggt cctggaacac aggtgcccct tggcctttcc
     5881 acctccctcc ttggggctgt ttgcatccct gccaggagcc ttaaaccagc cacctactgc
     5941 tgaagtctct tgtaacattc caaatctatg tgacacactg tataatgatg aatatttaat
     6001 gtgaagctct taatatacta caagtgtctt gttta
//
