LOCUS       NM_001372044            7691 bp    mRNA    linear   PRI 24-OCT-2024
DEFINITION  Homo sapiens SH3 and multiple ankyrin repeat domains 3 (SHANK3),
            mRNA.
ACCESSION   NM_001372044 NM_033517
VERSION     NM_001372044.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7691)
  AUTHORS   Fischer,I., Shohat,S., Leichtmann-Bardoogo,Y., Nayak,R., Wiener,G.,
            Rosh,I., Shemen,A., Tripathi,U., Rokach,M., Bar,E., Hussein,Y.,
            Castro,A.C., Chen,G., Soffer,A., Schokoroy-Trangle,S.,
            Elad-Sfadia,G., Assaf,Y., Schroeder,A., Monteiro,P., Stern,S.,
            Maoz,B.M. and Barak,B.
  TITLE     Shank3 mutation impairs glutamate signaling and myelination in ASD
            mouse model and human iPSC-derived OPCs
  JOURNAL   Sci Adv 10 (41), eadl4573 (2024)
   PUBMED   39392881
  REMARK    GeneRIF: Shank3 mutation impairs glutamate signaling and
            myelination in ASD mouse model and human iPSC-derived OPCs.
REFERENCE   2  (bases 1 to 7691)
  AUTHORS   Esmel-Vilomara,R., Dougherty-De Miguel,L., Artigas-Baleri,A.,
            Turon-Vinas,E., Cusco,I., Diaz-Gomez,A., Panades-De Oliveira,L.,
            Rocamora,R. and Boronat,S.
  TITLE     Cardiovascular abnormalities in patients with SHANK3 pathogenic
            variants: Beyond neurodevelopmental disorders and epilepsy
  JOURNAL   Eur J Med Genet 71, 104965 (2024)
   PUBMED   39094681
  REMARK    GeneRIF: Cardiovascular abnormalities in patients with SHANK3
            pathogenic variants: Beyond neurodevelopmental disorders and
            epilepsy.
REFERENCE   3  (bases 1 to 7691)
  AUTHORS   Lu,X., Ni,P., Suarez-Meade,P., Ma,Y., Forrest,E.N., Wang,G.,
            Wang,Y., Quinones-Hinojosa,A., Gerstein,M. and Jiang,Y.H.
  TITLE     Transcriptional determinism and stochasticity contribute to the
            complexity of autism-associated SHANK family genes
  JOURNAL   Cell Rep 43 (7), 114376 (2024)
   PUBMED   38900637
  REMARK    GeneRIF: Transcriptional determinism and stochasticity contribute
            to the complexity of autism-associated SHANK family genes.
REFERENCE   4  (bases 1 to 7691)
  AUTHORS   Waga,C., Okamoto,N., Ondo,Y., Fukumura-Kato,R., Goto,Y., Kohsaka,S.
            and Uchino,S.
  TITLE     Novel variants of the SHANK3 gene in Japanese autistic patients
            with severe delayed speech development
  JOURNAL   Psychiatr Genet 21 (4), 208-211 (2011)
   PUBMED   21378602
  REMARK    GeneRIF: The SHANK3 gene was analyzed in 128 autistic patients with
            manifestations similar to those seen in the 22q13.3 deletion
            syndrome.
REFERENCE   5  (bases 1 to 7691)
  AUTHORS   Kolevzon,A., Cai,G., Soorya,L., Takahashi,N., Grodberg,D.,
            Kajiwara,Y., Willner,J.P., Tryfon,A. and Buxbaum,J.D.
  TITLE     Analysis of a purported SHANK3 mutation in a boy with autism:
            clinical impact of rare variant research in neurodevelopmental
            disabilities
  JOURNAL   Brain Res 1380, 98-105 (2011)
   PUBMED   21062623
  REMARK    GeneRIF: [review] The presumptive exon containing the variant in
            the neurodevelopmentally disabled autism case highlighted in this
            review is not likely to be present in most or all SHANK3
            transcripts.
            Review article
REFERENCE   6  (bases 1 to 7691)
  AUTHORS   Durand,C.M., Betancur,C., Boeckers,T.M., Bockmann,J., Chaste,P.,
            Fauchereau,F., Nygren,G., Rastam,M., Gillberg,I.C., Anckarsater,H.,
            Sponheim,E., Goubran-Botros,H., Delorme,R., Chabane,N.,
            Mouren-Simeoni,M.C., de Mas,P., Bieth,E., Roge,B., Heron,D.,
            Burglen,L., Gillberg,C., Leboyer,M. and Bourgeron,T.
  TITLE     Mutations in the gene encoding the synaptic scaffolding protein
            SHANK3 are associated with autism spectrum disorders
  JOURNAL   Nat Genet 39 (1), 25-27 (2007)
   PUBMED   17173049
  REMARK    GeneRIF: Mutation of a single copy of SHANK3 on chromosome 22q13
            can result in language and/or social communication disorders.
REFERENCE   7  (bases 1 to 7691)
  AUTHORS   Wilson,H.L., Wong,A.C., Shaw,S.R., Tse,W.Y., Stapleton,G.A.,
            Phelan,M.C., Hu,S., Marshall,J. and McDermid,H.E.
  TITLE     Molecular characterisation of the 22q13 deletion syndrome supports
            the role of haploinsufficiency of SHANK3/PROSAP2 in the major
            neurological symptoms
  JOURNAL   J Med Genet 40 (8), 575-584 (2003)
   PUBMED   12920066
  REMARK    GeneRIF: Haploinsufficiency of the gene SHANK3, which codes for a
            structural protein of the postsynaptic density, may be a major
            causative factor in the neurological symptoms of 22q13 deletion
            syndrome.
REFERENCE   8  (bases 1 to 7691)
  AUTHORS   Lim,S., Naisbitt,S., Yoon,J., Hwang,J.I., Suh,P.G., Sheng,M. and
            Kim,E.
  TITLE     Characterization of the Shank family of synaptic proteins. Multiple
            genes, alternative splicing, and differential expression in brain
            and development
  JOURNAL   J Biol Chem 274 (41), 29510-29518 (1999)
   PUBMED   10506216
REFERENCE   9  (bases 1 to 7691)
  AUTHORS   Boeckers,T.M., Kreutz,M.R., Winter,C., Zuschratter,W., Smalla,K.H.,
            Sanmarti-Vila,L., Wex,H., Langnaese,K., Bockmann,J., Garner,C.C.
            and Gundelfinger,E.D.
  TITLE     Proline-rich synapse-associated protein-1/cortactin binding protein
            1 (ProSAP1/CortBP1) is a PDZ-domain protein highly enriched in the
            postsynaptic density
  JOURNAL   J Neurosci 19 (15), 6506-6518 (1999)
   PUBMED   10414979
REFERENCE   10 (bases 1 to 7691)
  AUTHORS   Phelan,K., Rogers,R.C. and Boccuto,L.
  TITLE     Phelan-McDermid Syndrome-SHANK3 Related
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301377
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC000050.22, AB569469.1 and
            AC000036.5.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Mar 3, 2020 this sequence version replaced NM_001372044.1.
            
            Summary: This gene is a member of the Shank gene family. Shank
            proteins are multidomain scaffold proteins of the postsynaptic
            density that connect neurotransmitter receptors, ion channels, and
            other membrane proteins to the actin cytoskeleton and
            G-protein-coupled signaling pathways. Shank proteins also play a
            role in synapse formation and dendritic spine maturation. Mutations
            in this gene are a cause of autism spectrum disorder (ASD), which
            is characterized by impairments in social interaction and
            communication, and restricted behavioral patterns and interests.
            Mutations in this gene also cause schizophrenia type 15, and are a
            major causative factor in the neurological symptoms of 22q13.3
            deletion syndrome, which is also known as Phelan-McDermid syndrome.
            Additional isoforms have been described for this gene but they have
            not yet been experimentally verified. [provided by RefSeq, Mar
            2012].
            
            Sequence Note: CAGE data and conservation lead to an extension of
            the 5' end of NM_033517.1. Coupled with a 2 nt indel in the
            reference genome sequence, this allowed the protein to extend 75 aa
            further N-terminally, so a new record was needed (NM_001372044.1).
            This further 5' UTR extension is creating NM_001372044.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: partial sample support SAMEA1968540, SAMEA1970526
                              [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000710353.1/ ENSP00000518224.1
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-31                AC000050.22        9014-9044
            32-535              AC000050.22        10267-10770
            536-595             AC000050.22        10773-10832
            596-5791            AB569469.1         1-5196
            5792-7691           AC000036.5         33563-35462
FEATURES             Location/Qualifiers
     source          1..7691
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="22"
                     /map="22q13.33"
     gene            1..7691
                     /gene="SHANK3"
                     /gene_synonym="DEL22q13.3; PROSAP2; PSAP2; SCZD15;
                     SPANK-2"
                     /note="SH3 and multiple ankyrin repeat domains 3"
                     /db_xref="GeneID:85358"
                     /db_xref="HGNC:HGNC:14294"
                     /db_xref="MIM:606230"
     exon            1..31
                     /gene="SHANK3"
                     /gene_synonym="DEL22q13.3; PROSAP2; PSAP2; SCZD15;
                     SPANK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            32..658
                     /gene="SHANK3"
                     /gene_synonym="DEL22q13.3; PROSAP2; PSAP2; SCZD15;
                     SPANK-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             371..5791
                     /gene="SHANK3"
                     /gene_synonym="DEL22q13.3; PROSAP2; PSAP2; SCZD15;
                     SPANK-2"
                     /note="shank postsynaptic density protein; proline rich
                     synapse associated protein 2; shank3 postsynaptic density
                     protein"
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein
                     3"
                     /protein_id="NP_001358973.1"
                     /db_xref="GeneID:85358"
                     /db_xref="HGNC:HGNC:14294"
                     /db_xref="MIM:606230"
                     /translation="MQLSRAAAAAAAAPAEPPEPLSPAPAPAPAPPGPLPRSAADGAP
                     AGGKGGPGRRAESPGAPFPGASGPGPGPGAGMDGPGASAVVVRVGIPDLQQTKCLRLD
                     PAAPVWAAKQRVLCALNHSLQDALNYGLFQPPSRGRAGKFLDEERLLQEYPPNLDTPL
                     PYLEFRYKRRVYAQNLIDDKQFAKLHTKANLKKFMDYVQLHSTDKVARLLDKGLDPNF
                     HDPDSGECPLSLAAQLDNATDLLKVLKNGGAHLDFRTRDGLTAVHCATRQRNAAALTT
                     LLDLGASPDYKDSRGLTPLYHSALGGGDALCCELLLHDHAQLGITDENGWQEIHQACR
                     FGHVQHLEHLLFYGADMGAQNASGNTALHICALYNQESCARVLLFRGANRDVRNYNSQ
                     TAFQVAIIAGNFELAEVIKTHKDSDVVPFRETPSYAKRRRLAGPSGLASPRPLQRSAS
                     DINLKGEAQPAASPGPSLRSLPHQLLLQRLQEEKDRDRDADQESNISGPLAGRAGQSK
                     ISPSGPGGPGPAPGPGPAPPAPPAPPPRGPKRKLYSAVPGRKFIAVKAHSPQGEGEIP
                     LHRGEAVKVLSIGEGGFWEGTVKGRTGWFPADCVEEVQMRQHDTRPETREDRTKRLFR
                     HYTVGSYDSLTSHSDYVIDDKVAVLQKRDHEGFGFVLRGAKAETPIEEFTPTPAFPAL
                     QYLESVDVEGVAWRAGLRTGDFLIEVNGVNVVKVGHKQVVALIRQGGNRLVMKVVSVT
                     RKPEEDGARRRAPPPPKRAPSTTLTLRSKSMTAELEELASIRRRKGEKLDEMLAAAAE
                     PTLRPDIADADSRAATVKQRPTSRRITPAEISSLFERQGLPGPEKLPGSLRKGIPRTK
                     SVGEDEKLASLLEGRFPRSTSMQDPVREGRGIPPPPQTAPPPPPAPYYFDSGPPPAFS
                     PPPPPGRAYDTVRSSFKPGLEARLGAGAAGLYEPGAALGPLPYPERQKRARSMIILQD
                     SAPESGDAPRPPPAATPPERPKRRPRPPGPDSPYANLGAFSASLFAPSKPQRRKSPLV
                     KQLQVEDAQERAALAVGSPGPGGGSFAREPSPTHRGPRPGGLDYGAGDGPGLAFGGPG
                     PAKDRRLEERRRSTVFLSVGAIEGSAPGADLPSLQPSRSIDERLLGTGPTAGRDLLLP
                     SPVSALKPLVSGPSLGPSGSTFIHPLTGKPLDPSSPLALALAARERALASQAPSRSPT
                     PVHSPDADRPGPLFVDVQARDPERGSLASPAFSPRSPAWIPVPARREAEKVPREERKS
                     PEDKKSMILSVLDTSLQRPAGLIVVHATSNGQEPSRLGGAEEERPGTPELAPAPMQSA
                     AVAEPLPSPRAQPPGGTPADAGPGQGSSEEEPELVFAVNLPPAQLSSSDEETREELAR
                     IGLVPPPEEFANGVLLATPLAGPGPSPTTVPSPASGKPSSEPPPAPESAADSGVEEAD
                     TRSSSDPHLETTSTISTVSSMSTLSSESGELTDTHTSFADGHTFLLEKPPVPPKPKLK
                     SPLGKGPVTFRDPLLKQSSDSELMAQQHHAASAGLASAAGPARPRYLFQRRSKLWGDP
                     VESRGLPGPEDDKPTVISELSSRLQQLNKDTRSLGEEPVGGLGSLLDPAKKSPIAAAR
                     LFSSLGELSSISAQRSPGGPGGGASYSVRPSGRYPVARRAPSPVKPASLERVEGLGAG
                     AGGAGRPFGLTPPTILKSSSLSIPHEPKEVRFVVRSVSARSRSPSPSPLPSPASGPGP
                     GAPGPRRPFQQKPLQLWSKFDVGDWLESIHLGEHRDRFEDHEIEGAHLPALTKDDFVE
                     LGVTRVGHRMNIERALRQLDGS"
     exon            659..862
                     /gene="SHANK3"
                     /gene_synonym="DEL22q13.3; PROSAP2; PSAP2; SCZD15;
                     SPANK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            863..934
                     /gene="SHANK3"
                     /gene_synonym="DEL22q13.3; PROSAP2; PSAP2; SCZD15;
                     SPANK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            935..1043
                     /gene="SHANK3"
                     /gene_synonym="DEL22q13.3; PROSAP2; PSAP2; SCZD15;
                     SPANK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1044..1195
                     /gene="SHANK3"
                     /gene_synonym="DEL22q13.3; PROSAP2; PSAP2; SCZD15;
                     SPANK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1196..1363
                     /gene="SHANK3"
                     /gene_synonym="DEL22q13.3; PROSAP2; PSAP2; SCZD15;
                     SPANK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1364..1480
                     /gene="SHANK3"
                     /gene_synonym="DEL22q13.3; PROSAP2; PSAP2; SCZD15;
                     SPANK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1481..1558
                     /gene="SHANK3"
                     /gene_synonym="DEL22q13.3; PROSAP2; PSAP2; SCZD15;
                     SPANK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1559..1625
                     /gene="SHANK3"
                     /gene_synonym="DEL22q13.3; PROSAP2; PSAP2; SCZD15;
                     SPANK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1626..1899
                     /gene="SHANK3"
                     /gene_synonym="DEL22q13.3; PROSAP2; PSAP2; SCZD15;
                     SPANK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1900..2093
                     /gene="SHANK3"
                     /gene_synonym="DEL22q13.3; PROSAP2; PSAP2; SCZD15;
                     SPANK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2094..2207
                     /gene="SHANK3"
                     /gene_synonym="DEL22q13.3; PROSAP2; PSAP2; SCZD15;
                     SPANK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2208..2283
                     /gene="SHANK3"
                     /gene_synonym="DEL22q13.3; PROSAP2; PSAP2; SCZD15;
                     SPANK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2284..2366
                     /gene="SHANK3"
                     /gene_synonym="DEL22q13.3; PROSAP2; PSAP2; SCZD15;
                     SPANK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2367..2491
                     /gene="SHANK3"
                     /gene_synonym="DEL22q13.3; PROSAP2; PSAP2; SCZD15;
                     SPANK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2492..2624
                     /gene="SHANK3"
                     /gene_synonym="DEL22q13.3; PROSAP2; PSAP2; SCZD15;
                     SPANK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2625..2705
                     /gene="SHANK3"
                     /gene_synonym="DEL22q13.3; PROSAP2; PSAP2; SCZD15;
                     SPANK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2706..2729
                     /gene="SHANK3"
                     /gene_synonym="DEL22q13.3; PROSAP2; PSAP2; SCZD15;
                     SPANK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2730..2860
                     /gene="SHANK3"
                     /gene_synonym="DEL22q13.3; PROSAP2; PSAP2; SCZD15;
                     SPANK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2861..2945
                     /gene="SHANK3"
                     /gene_synonym="DEL22q13.3; PROSAP2; PSAP2; SCZD15;
                     SPANK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2946..5199
                     /gene="SHANK3"
                     /gene_synonym="DEL22q13.3; PROSAP2; PSAP2; SCZD15;
                     SPANK-2"
                     /inference="alignment:Splign:2.1.0"
     exon            5200..7691
                     /gene="SHANK3"
                     /gene_synonym="DEL22q13.3; PROSAP2; PSAP2; SCZD15;
                     SPANK-2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 acacaccgca ctggaggctc cgctctgccc gctccgggac cccttcctcc gccgcacccg
       61 ccccggtggt ccgcggatgc ccgcccttgc cgctcagcca ctccccccgc accgaggcct
      121 aggactcccc cccccaaccc cgtcacagcc ccccagaccc ccgccccgtg gctcggcccc
      181 cgccctccgc acacacctcc cgcccccacc cgggaccccg caagtaaccc cccagcactg
      241 gccctgagcc ctcccggccc ccgcctccgg cgcagccccc tcgccacccc cgcttccctc
      301 ccgtctcagg ccccctcccc ccgccgcccc cgcccccggg gaaggcaggc gccgagctga
      361 gccggggccg atgcagctga gccgcgccgc cgccgccgcc gccgccgccc ctgcggagcc
      421 cccggagccg ctgtcccccg cgccggcccc ggccccggcc ccccccggcc ccctcccgcg
      481 cagcgcggcc gacggggctc cggcgggggg gaaggggggg ccggggcgcc gcgcggagtc
      541 cccgggcgct ccgttccccg gcgcgagcgg ccccggcccg ggccccggcg cggggatgga
      601 cggccccggg gccagcgccg tggtcgtgcg cgtcggcatc ccggacctgc agcagacgaa
      661 gtgcctgcgc ctggacccgg ccgcgcccgt gtgggccgcc aagcagcgcg tgctctgcgc
      721 cctcaaccac agcctccagg acgcgctcaa ctatgggctt ttccagccgc cctcccgggg
      781 ccgcgccggc aagttcctgg atgaggagcg gctcctgcag gagtacccgc ccaacctgga
      841 cacgcccctg ccctacctgg agtttcgata caagcggcga gtttatgccc agaacctcat
      901 cgatgataag cagtttgcaa agcttcacac aaaggcgaac ctgaagaagt tcatggacta
      961 cgtccagctg catagcacgg acaaggtggc acgcctgttg gacaaggggc tggaccccaa
     1021 cttccatgac cctgactcag gagagtgccc cctgagcctc gcagcccagc tggacaacgc
     1081 cacggacctg ctaaaggtgc tgaagaatgg tggtgcccac ctggacttcc gcactcgcga
     1141 tgggctcact gccgtgcact gtgccacacg ccagcggaat gcggcagcac tgacgaccct
     1201 gctggacctg ggggcttcac ctgactacaa ggacagccgc ggcttgacac ccctctacca
     1261 cagcgccctg gggggtgggg atgccctctg ctgtgagctg cttctccacg accacgctca
     1321 gctggggatc accgacgaga atggctggca ggagatccac caggcctgcc gctttgggca
     1381 cgtgcagcat ctggagcacc tgctgttcta tggggcagac atgggggccc agaacgcctc
     1441 ggggaacaca gccctgcaca tctgtgccct ctacaaccag gagagctgtg ctcgtgtcct
     1501 gctcttccgt ggagctaaca gggatgtccg caactacaac agccagacag ccttccaggt
     1561 ggccatcatc gcagggaact ttgagcttgc agaggttatc aagacccaca aagactcgga
     1621 tgttgtacca ttcagggaaa cccccagcta tgcgaagcgg cggcgactgg ctggccccag
     1681 tggcttggca tcccctcggc ctctgcagcg ctcagccagc gatatcaacc tgaaggggga
     1741 ggcacagcca gcagcttctc ctggaccctc gctgagaagc ctcccccacc agctgctgct
     1801 ccagcggctg caagaggaga aagatcgtga ccgggatgcc gaccaggaga gcaacatcag
     1861 tggcccttta gcaggcaggg ccggccaaag caagatcagc ccgagcgggc ccggcggccc
     1921 cggccccgcg cccggccccg gccccgcgcc ccctgcgccc cccgcaccgc cgccccgggg
     1981 cccgaagcgg aaactttaca gcgccgtccc cggccgcaag ttcatcgccg tgaaggcgca
     2041 cagcccgcag ggtgaaggcg agatcccgct gcaccgcggc gaggccgtga aggtgctcag
     2101 cattggggag ggcggtttct gggagggaac cgtgaaaggc cgcacgggct ggttcccggc
     2161 cgactgcgtg gaggaagtgc agatgaggca gcatgacaca cggcctgaaa cgcgggagga
     2221 ccggacgaag cggctctttc ggcactacac agtgggctcc tacgacagcc tcacctcaca
     2281 cagcgattat gtcattgatg acaaagtggc tgtcctgcag aaacgggacc acgagggctt
     2341 tggttttgtg ctccggggag ccaaagcaga gacccccatc gaggagttca cgcccacgcc
     2401 agccttcccg gcgctgcagt atctcgagtc ggtggacgtg gagggtgtgg cctggagggc
     2461 cgggctgcgc acgggagact tcctcatcga ggtgaacggg gtgaacgtgg tgaaggtcgg
     2521 acacaagcag gtggtggctc tgattcgcca gggtggcaac cgcctcgtca tgaaggttgt
     2581 gtctgtgaca aggaagccag aagaggacgg ggctcggcgc agagccccac cgccccccaa
     2641 gagggccccc agcaccacac tgaccctgcg ctccaagtcc atgacagctg agctcgagga
     2701 acttgcctcc attcggagaa gaaaagggga gaagctggac gagatgctgg cagccgccgc
     2761 agagccaacg ctgcggccag acatcgcaga cgcagactcc agagccgcca ccgtcaaaca
     2821 gaggcccacc agtcggagga tcacacccgc cgagattagc tcattgtttg aacgccaggg
     2881 cctcccaggc ccagagaagc tgccgggctc cttgcggaag gggattccac ggaccaagtc
     2941 tgtaggggag gacgagaagc tggcgtccct gctggaaggg cgcttcccgc ggagcacctc
     3001 gatgcaagac ccggtgcgcg agggtcgcgg catcccgccc ccgccgcaga ccgcgccgcc
     3061 tcccccgccc gcgccctact acttcgactc ggggccgccc ccggccttct cgccgccgcc
     3121 cccgccgggc cgcgcctacg acacggtgcg ctccagcttc aagcccggcc tggaggcgcg
     3181 cctgggcgcg ggcgctgccg gcctgtacga gccgggcgcg gccctcggcc cgctgccgta
     3241 tcccgagcgg cagaagcgcg cgcgctccat gatcatcctg caggactcgg cgcccgagtc
     3301 gggcgacgcc cctcgacccc cgcccgcggc caccccgccc gagcgaccca agcgccggcc
     3361 gcggccgccc ggccccgaca gcccctacgc caacctgggc gccttcagcg ccagcctctt
     3421 cgctccgtcc aagccgcagc gccgcaagag ccccctggtg aagcagctgc aggtggagga
     3481 cgcgcaggag cgcgcggccc tggccgtggg cagccccggt cccggcggcg gcagcttcgc
     3541 ccgcgagccc tccccgaccc accgcggtcc gcgcccgggt ggcctcgact acggcgcggg
     3601 cgatggcccg gggctcgcgt tcggcggccc gggcccggcc aaggaccggc ggctggagga
     3661 gcggcgccgc tccactgtgt tcctgtccgt gggggccatc gagggcagcg cccccggcgc
     3721 ggatctgcca tccctacagc cctcccgctc catcgacgag cgcctcctgg ggaccggccc
     3781 caccgccggc cgcgacctgc tgctgccctc cccggtgtct gccctgaagc cgttggtcag
     3841 cggcccgagc ctggggccct cgggttccac cttcatccac ccactcaccg gcaaacccct
     3901 ggaccccagc tcacccctgg cccttgccct ggctgcccga gagcgagctc tggcctccca
     3961 ggcgccctcc cggtccccca cacccgtgca cagtcccgac gccgaccgcc ccggacccct
     4021 gtttgtggat gtacaggccc gggacccaga gcgagggtcc ctggcttccc cggctttctc
     4081 cccacggagc ccagcctgga ttcctgtgcc tgctcgcagg gaggcagaga aggtcccccg
     4141 ggaggagcgg aagtcacccg aggacaagaa gtccatgatc ctcagcgtcc tggacacatc
     4201 cctgcagcgg ccagctggcc tcatcgttgt gcacgccacc agcaacgggc aggagcccag
     4261 caggctgggg ggggccgaag aggagcgccc gggcaccccg gagttggccc cggcccccat
     4321 gcagtcagcg gctgtggcag agcccctgcc cagcccccgg gcccagcccc ctggtggcac
     4381 cccggcagac gccgggccag gccagggcag ctcagaggaa gagccagagc tggtgtttgc
     4441 tgtgaacctg ccacctgccc agctgtcgtc cagcgatgag gagaccaggg aggagctggc
     4501 ccgaattggg ttggtgccac cccctgaaga gtttgccaac ggggtcctgc tggccacccc
     4561 actcgctggc ccgggcccct cgcccaccac ggtgcccagc ccggcctcag ggaagcccag
     4621 cagtgagcca ccccctgccc ctgagtctgc agccgactct ggggtggagg aggctgacac
     4681 acgcagctcc agcgaccccc acctggagac cacaagcacc atctccacgg tgtccagcat
     4741 gtccaccttg agctcggaga gcggggaact cactgacacc cacacctcct tcgctgacgg
     4801 acacactttt ctactcgaga agccaccagt gcctcccaag cccaagctca agtccccgct
     4861 ggggaagggg ccggtgacct tcagggaccc gctgctgaag cagtcctcgg acagcgagct
     4921 catggcccag cagcaccacg ccgcctctgc cgggctggcc tctgccgccg ggcctgcccg
     4981 ccctcgctac ctcttccaga gaaggtccaa gctatggggg gaccccgtgg agagccgggg
     5041 gctccctggg cctgaagacg acaaaccaac tgtgatcagt gagctcagct cccgcctgca
     5101 gcagctgaac aaggacacgc gttccctggg ggaggaacca gttggtggcc tgggcagcct
     5161 gctggaccct gccaagaagt cgcccatcgc agcagctcgg ctcttcagca gcctcggtga
     5221 gctgagctcc atttcagcgc agcgcagccc cgggggcccg ggcggcgggg cctcgtactc
     5281 ggtgaggccc agtggccgct accccgtggc gagacgcgcc ccgagcccgg tgaagcccgc
     5341 gtcgctggag cgggtggagg ggctgggggc gggcgcgggg ggcgcagggc ggcccttcgg
     5401 cctcacgccc cccaccatcc tcaagtcgtc cagcctctcc atcccgcacg agcccaagga
     5461 ggtgcgcttc gtggtgcgca gcgtgagcgc gcgcagtcgc tccccctcgc cgtcgccgct
     5521 gccctcgccc gcgtccggcc ccggccccgg cgcccccggc ccacgccgac ccttccagca
     5581 gaagccgctg cagctctgga gcaagttcga cgtgggcgac tggctggaga gcatccacct
     5641 aggcgagcac cgcgaccgct tcgaggacca tgagatagaa ggcgcgcacc tacccgcgct
     5701 taccaaggac gacttcgtgg agctgggcgt cacgcgcgtg ggccaccgca tgaacatcga
     5761 gcgcgcgctc aggcagctgg acggcagctg acgccccacc cccactcccg ccccggccgt
     5821 gccctgccgg cagggccccc cacccccacc ccgggccgcg ggctcggcct gccccttacg
     5881 acggcgcccg ggccaggaat gttgcatgaa tcgtcctgtt tgctgttgct cggagactcg
     5941 ccctgtacat tgcttagtgc cctcaccggc cgcccagccc acccagcgca cagtcaggaa
     6001 gggcgtggac cagggaggct ggggcgggag gtgccggggg tggggtgccc tagcgtgacc
     6061 acctccttcg cagctcctgg tggccattct cccagagggg gaacctagtc cagcatgcga
     6121 ggtcaggacc cgccttggtg actcgggggg aggggggaga cattgggatt ctcgatgggg
     6181 gccaaggagc ccccctgttt tgcatatttt aatccactct atatttggaa cgagaaaagg
     6241 aacaaatatc tctgtccgta atagtttcct ctcccctccc ttctacttcc actggtccca
     6301 ctgcagctgc ccagtcttcc atctccggcc cctcactgcc actgccaccc cacaacgggg
     6361 caggggacgc tccagctggt ctggggttgg ccagggccct agtggcccgc cctggggccc
     6421 cagctcggcc cctcgcctcg ctgagctcta gtgtgcccca ccgacccttc aggtgctgct
     6481 cgtggtggga ggggcggcag gccgcgggtc ctgctgtgca cccgcgggac cagccggcct
     6541 gggagaccat cggccggggg ggatgagggc agggccctgc cgctccaccg cagccatctt
     6601 cctcacaggg tctctcccca aggagggggc tagcttggtc cccatgctct tgggcaacta
     6661 cagcagagaa gcctccctgc cttggacccc aaagtctcct gtcctgccct ttatgtgtgt
     6721 gggtgaaact gggtgcgtct gagcacgtgg gagccgtgtg tgtgcctgat tactgagtgg
     6781 ccaccagggg ccgctctgga ctagcgcggg gccgtggagg cgtgcaccgt gtgcatgcgt
     6841 ggggtgtacc tgtgagagca ccctgtctcc tcttccaaag aaagtcagag gccatcctgc
     6901 accctgggtc cagctgtttg cccagcctgt ccttccagag cctcacccag cctgagcggg
     6961 gttccctggt gaatccctgc tgcttgggga ggccccaagg gccccttgga ggcagcgccc
     7021 ccaccttggg cttctgaggg catcataggg ggacccctag agtcagttca ccacaggccc
     7081 tggggagagt caaagacccc cgagggtgcc cagcccccca cactgtgact cctcacactc
     7141 agcgatgacc tgtggggtgg ggggccctgg gacgttttta aacctagggt ttggagtctg
     7201 gactaagctc catccacgtc actcacaagt ttctgtttat atttctagct ttttttaata
     7261 aaataaaaaa aaaaagaaaa cagaagtttt cacaacccag gggcctggca cgccggtctg
     7321 tgcctgcccg ccccgccctg gcccaccggc cccactccct gggcacagag tcacacccac
     7381 tcatccttcc gccaacagtc caggtcacac agcagcagtc actgtaacag actgccacat
     7441 acacactcgg tctcacactc acctgtgggt tttggttccg ttcaatttgg gtttttaact
     7501 ttacagggtc agttccgctt cacctccttt tgtatggagt tccatccggg gggtttcacc
     7561 ccctgctcca gtcctgaggc ctcctgaccc tgacgttgtg atacgcccca cagagatcta
     7621 tgtttcttat attattatta ttgataataa ttattataat attattatgt aataaattta
     7681 taagaaatga a
//
