LOCUS       NM_012309              10830 bp    mRNA    linear   PRI 15-OCT-2024
DEFINITION  Homo sapiens SH3 and multiple ankyrin repeat domains 2 (SHANK2),
            transcript variant 1, mRNA.
ACCESSION   NM_012309 XM_374904 XM_928457 XM_939728
VERSION     NM_012309.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10830)
  AUTHORS   Lai,W., Zhao,Y., Chen,Y., Dai,Z., Chen,R., Niu,Y., Chen,X.,
            Chen,S., Huang,G., Shan,Z., Zheng,J., Hu,Y., Chen,Q., Gong,S.,
            Kang,S., Guo,H., Ma,X., Song,Y., Xia,K., Wang,J., Zhou,L., So,K.F.,
            Wang,K., Qiu,S., Zhang,L., Chen,J. and Shi,L.
  TITLE     Autism patient-derived SHANK2BY29X mutation affects the development
            of ALDH1A1 negative dopamine neuron
  JOURNAL   Mol Psychiatry 29 (10), 3180-3194 (2024)
   PUBMED   38704506
  REMARK    GeneRIF: Autism patient-derived SHANK2B[Y29X] mutation affects the
            development of ALDH1A1 negative dopamine neuron.
REFERENCE   2  (bases 1 to 10830)
  AUTHORS   Hassani Nia,F., Woike,D., Bento,I., Niebling,S., Tibbe,D.,
            Schulz,K., Hirnet,D., Skiba,M., Honck,H.H., Veith,K., Gunther,C.,
            Scholz,T., Bierhals,T., Driemeyer,J., Bend,R., Failla,A.V.,
            Lohr,C., Alai,M.G. and Kreienkamp,H.J.
  TITLE     Structural deficits in key domains of Shank2 lead to alterations in
            postsynaptic nanoclusters and to a neurodevelopmental disorder in
            humans
  JOURNAL   Mol Psychiatry 29 (6), 1683-1697 (2024)
   PUBMED   36450866
  REMARK    GeneRIF: Structural deficits in key domains of Shank2 lead to
            alterations in postsynaptic nanoclusters and to a
            neurodevelopmental disorder in humans.
REFERENCE   3  (bases 1 to 10830)
  AUTHORS   Woelfle,S., Pedro,M.T., Wagner,J., Schon,M. and Boeckers,T.M.
  TITLE     Expression profiles of the autism-related SHANK proteins in the
            human brain
  JOURNAL   BMC Biol 21 (1), 254 (2023)
   PUBMED   37953224
  REMARK    GeneRIF: Expression profiles of the autism-related SHANK proteins
            in the human brain.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 10830)
  AUTHORS   Li,N., Ren,C., Li,S., Yu,W., Jin,K. and Ji,X.
  TITLE     Remote ischemic conditioning alleviates chronic cerebral
            hypoperfusion-induced cognitive decline and synaptic dysfunction
            via the miR-218a-5p/SHANK2 pathway
  JOURNAL   Prog Neurobiol 230, 102514 (2023)
   PUBMED   37574039
  REMARK    GeneRIF: Remote ischemic conditioning alleviates chronic cerebral
            hypoperfusion-induced cognitive decline and synaptic dysfunction
            via the miR-218a-5p/SHANK2 pathway.
REFERENCE   5  (bases 1 to 10830)
  AUTHORS   Chang,C.F., Huang,S.P., Hsueh,Y.M., Geng,J.H., Huang,C.Y. and
            Bao,B.Y.
  TITLE     Genetic Analysis Implicates Dysregulation of SHANK2 in Renal Cell
            Carcinoma Progression
  JOURNAL   Int J Environ Res Public Health 19 (19), 12471 (2022)
   PUBMED   36231770
  REMARK    GeneRIF: Genetic Analysis Implicates Dysregulation of SHANK2 in
            Renal Cell Carcinoma Progression.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 10830)
  AUTHORS   Berkel,S., Marshall,C.R., Weiss,B., Howe,J., Roeth,R., Moog,U.,
            Endris,V., Roberts,W., Szatmari,P., Pinto,D., Bonin,M., Riess,A.,
            Engels,H., Sprengel,R., Scherer,S.W. and Rappold,G.A.
  TITLE     Mutations in the SHANK2 synaptic scaffolding gene in autism
            spectrum disorder and mental retardation
  JOURNAL   Nat Genet 42 (6), 489-491 (2010)
   PUBMED   20473310
  REMARK    GeneRIF: we identified de novo copy number variations in the SHANK2
            synaptic scaffolding gene in two unrelated individuals with
            autism-spectrum disorder (ASD) and mental retardation
            GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   7  (bases 1 to 10830)
  AUTHORS   Lim,S., Naisbitt,S., Yoon,J., Hwang,J.I., Suh,P.G., Sheng,M. and
            Kim,E.
  TITLE     Characterization of the Shank family of synaptic proteins. Multiple
            genes, alternative splicing, and differential expression in brain
            and development
  JOURNAL   J Biol Chem 274 (41), 29510-29518 (1999)
   PUBMED   10506216
REFERENCE   8  (bases 1 to 10830)
  AUTHORS   Tu,J.C., Xiao,B., Naisbitt,S., Yuan,J.P., Petralia,R.S.,
            Brakeman,P., Doan,A., Aakalu,V.K., Lanahan,A.A., Sheng,M. and
            Worley,P.F.
  TITLE     Coupling of mGluR/Homer and PSD-95 complexes by the Shank family of
            postsynaptic density proteins
  JOURNAL   Neuron 23 (3), 583-592 (1999)
   PUBMED   10433269
REFERENCE   9  (bases 1 to 10830)
  AUTHORS   Zitzer,H., Richter,D. and Kreienkamp,H.J.
  TITLE     Agonist-dependent interaction of the rat somatostatin receptor
            subtype 2 with cortactin-binding protein 1
  JOURNAL   J Biol Chem 274 (26), 18153-18156 (1999)
   PUBMED   10373412
REFERENCE   10 (bases 1 to 10830)
  AUTHORS   Du,Y., Weed,S.A., Xiong,W.C., Marshall,T.D. and Parsons,J.T.
  TITLE     Identification of a novel cortactin SH3 domain-binding protein and
            its localization to growth cones of cultured neurons
  JOURNAL   Mol Cell Biol 18 (10), 5838-5851 (1998)
   PUBMED   9742101
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AP004370.2, AP005401.3,
            AC234421.2, AP003783.4, AP000590.7 and AP001271.4.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Mar 25, 2020 this sequence version replaced NM_012309.4.
            
            Summary: This gene encodes a protein that is a member of the Shank
            family of synaptic proteins that may function as molecular
            scaffolds in the postsynaptic density of excitatory synapses. Shank
            proteins contain multiple domains for protein-protein interaction,
            including ankyrin repeats, and an SH3 domain. This particular
            family member contains a PDZ domain, a consensus sequence for
            cortactin SH3 domain-binding peptides and a sterile alpha motif.
            The alternative splicing demonstrated in Shank genes has been
            suggested as a mechanism for regulating the molecular structure of
            Shank and the spectrum of Shank-interacting proteins in the
            postsynaptic densities of the adult and developing brain.
            Alterations in the encoded protein may be associated with
            susceptibility to autism spectrum disorder. Alternative splicing
            results in multiple transcript variants. [provided by RefSeq, Feb
            2014].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            CDS exon combination :: AB208025.1 [ECO:0000331]
            RNAseq introns       :: mixed sample support SAMEA1965299,
                                    SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000601538.6/ ENSP00000469689.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-153               AP004370.2         44526-44678         c
            154-253             AP004370.2         16798-16897         c
            254-472             AP005401.3         62945-63163         c
            473-676             AP005401.3         34654-34857         c
            677-748             AP005401.3         29118-29189         c
            749-857             AP005401.3         25766-25874         c
            858-1009            AC234421.2         2099-2250
            1010-1177           AC234421.2         4198-4365
            1178-1294           AC234421.2         21512-21628
            1295-1372           AC234421.2         40229-40306
            1373-1439           AP003783.4         113568-113634       c
            1440-1758           AP003783.4         37431-37749         c
            1759-1928           AP003783.4         24069-24238         c
            1929-2042           AP003783.4         15510-15623         c
            2043-2118           AP000590.7         54717-54792         c
            2119-2201           AP000590.7         17625-17707         c
            2202-2326           AP000590.7         15857-15981         c
            2327-2462           AP001271.4         47009-47144         c
            2463-2543           AP001271.4         46419-46499         c
            2544-2552           AP001271.4         46136-46144         c
            2553-2573           AP001271.4         44783-44803         c
            2574-2704           AP001271.4         36548-36678         c
            2705-2816           AP001271.4         34489-34600         c
            2817-2837           AP001271.4         33541-33561         c
            2838-5244           AP001271.4         29527-31933         c
            5245-10830          AP001271.4         12067-17652         c
FEATURES             Location/Qualifiers
     source          1..10830
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q13.3-q13.4"
     gene            1..10830
                     /gene="SHANK2"
                     /gene_synonym="AUTS17; CORTBP1; CTTNBP1; ProSAP1; SHANK;
                     SPANK-3"
                     /note="SH3 and multiple ankyrin repeat domains 2"
                     /db_xref="GeneID:22941"
                     /db_xref="HGNC:HGNC:14295"
                     /db_xref="MIM:603290"
     exon            1..153
                     /gene="SHANK2"
                     /gene_synonym="AUTS17; CORTBP1; CTTNBP1; ProSAP1; SHANK;
                     SPANK-3"
                     /inference="alignment:Splign:2.1.0"
     exon            154..253
                     /gene="SHANK2"
                     /gene_synonym="AUTS17; CORTBP1; CTTNBP1; ProSAP1; SHANK;
                     SPANK-3"
                     /inference="alignment:Splign:2.1.0"
     exon            254..472
                     /gene="SHANK2"
                     /gene_synonym="AUTS17; CORTBP1; CTTNBP1; ProSAP1; SHANK;
                     SPANK-3"
                     /inference="alignment:Splign:2.1.0"
     CDS             266..5815
                     /gene="SHANK2"
                     /gene_synonym="AUTS17; CORTBP1; CTTNBP1; ProSAP1; SHANK;
                     SPANK-3"
                     /note="isoform 1 is encoded by transcript variant 1;
                     cortactin SH3 domain-binding protein; GKAP/SAPAP
                     interacting protein; proline-rich synapse associated
                     protein 1; cortactin-binding protein 1"
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein
                     2 isoform 1"
                     /protein_id="NP_036441.2"
                     /db_xref="GeneID:22941"
                     /db_xref="HGNC:HGNC:14295"
                     /db_xref="MIM:603290"
                     /translation="MPRSPTSSEDEMAQSFSDYSVGSESDSSKEETIYDTIRATAEKP
                     GGARTEESQGNTLVIRVVIHDLQQTKCIRFNPDATVWVAKQRILCTLTQSLKDVLNYG
                     LFQPASNGRDGKFLDEERLLREYPQPVGEGVPSLEFRYKKRVYKQASLDEKQLAKLHT
                     KTNLKKCMDHIQHRLVEKITKMLDRGLDPNFHDPETGETPLTLAAQLDDSVEVIKALK
                     NGGAHLDFRAKDGMTALHKAARARNQVALKTLLELGASPDYKDSYGLTPLYHTAIVGG
                     DPYCCELLLHEHATVCCKDENGWHEIHQACRYGHVQHLEHLLFYGADMSAQNASGNTA
                     LHICALYNQDSCARVLLFRGGNKELKNYNSQTPFQVAIIAGNFELAEYIKNHKETDIV
                     PFREAPAYSNRRRRPPNTLAAPRVLLRSNSDNNLNASAPDWAVCSTATSHRSLSPQLL
                     QQMPSKPEGAAKTIGSYVPGPRSRSPSLNRLGGAGEDGKRPQPLWHVGSPFALGANKD
                     SLSAFEYPGPKRKLYSAVPGRLFVAVKPYQPQVDGEIPLHRGDRVKVLSIGEGGFWEG
                     SARGHIGWFPAECVEEVQCKPRDSQAETRADRSKKLFRHYTVGSYDSFDTSSDCIIEE
                     KTVVLQKKDNEGFGFVLRGAKADTPIEEFTPTPAFPALQYLESVDEGGVAWQAGLRTG
                     DFLIEVNNENVVKVGHRQVVNMIRQGGNHLVLKVVTVTRNLDPDDTARKKAPPPPKRA
                     PTTALTLRSKSMTSELEELVDKASVRKKKDKPEEIVPASKPSRAAENMAVEPRVATIK
                     QRPSSRCFPAGSDMNSVYERQGIAVMTPTVPGSPKAPFLGIPRGTMRRQKSIDSRIFL
                     SGITEEERQFLAPPMLKFTRSLSMPDTSEDIPPPPQSVPPSPPPPSPTTYNCPKSPTP
                     RVYGTIKPAFNQNSAAKVSPATRSDTVATMMREKGMYFRRELDRYSLDSEDLYSRNAG
                     PQANFRNKRGQMPENPYSEVGKIASKAVYVPAKPARRKGMLVKQSNVEDSPEKTCSIP
                     IPTIIVKEPSTSSSGKSSQGSSMEIDPQAPEPPSQLRPDESLTVSSPFAAAIAGAVRD
                     REKRLEARRNSPAFLSTDLGDEDVGLGPPAPRTRPSMFPEEGDFADEDSAEQLSSPMP
                     SATPREPENHFVGGAEASAPGEAGRPLNSTSKAQGPESSPAVPSASSGTAGPGNYVHP
                     LTGRLLDPSSPLALALSARDRAMKESQQGPKGEAPKADLNKPLYIDTKMRPSLDAGFP
                     TVTRQNTRGPLRRQETENKYETDLGRDRKGDDKKNMLIDIMDTSQQKSAGLLMVHTVD
                     ATKLDNALQEEDEKAEVEMKPDSSPSEVPEGVSETEGALQISAAPEPTTVPGRTIVAV
                     GSMEEAVILPFRIPPPPLASVDLDEDFIFTEPLPPPLEFANSFDIPDDRAASVPALSD
                     LVKQKKSDTPQSPSLNSSQPTNSADSKKPASLSNCLPASFLPPPESFDAVADSGIEEV
                     DSRSSSDHHLETTSTISTVSSISTLSSEGGENVDTCTVYADGQAFMVDKPPVPPKPKM
                     KPIIHKSNALYQDALVEEDVDSFVIPPPAPPPPPGSAQPGMAKVLQPRTSKLWGDVTE
                     IKSPILSGPKANVISELNSILQQMNREKLAKPGEGLDSPMGAKSASLAPRSPEIMSTI
                     SGTRSTTVTFTVRPGTSQPITLQSRPPDYESRTSGTRRAPSPVVSPTEMNKETLPAPL
                     SAATASPSPALSDVFSLPSQPPSGDLFGLNPAGRSRSPSPSILQQPISNKPFTTKPVH
                     LWTKPDVADWLESLNLGEHKEAFMDNEIDGSHLPNLQKEDLIDLGVTRVGHRMNIERA
                     LKQLLDR"
     exon            473..676
                     /gene="SHANK2"
                     /gene_synonym="AUTS17; CORTBP1; CTTNBP1; ProSAP1; SHANK;
                     SPANK-3"
                     /inference="alignment:Splign:2.1.0"
     exon            677..748
                     /gene="SHANK2"
                     /gene_synonym="AUTS17; CORTBP1; CTTNBP1; ProSAP1; SHANK;
                     SPANK-3"
                     /inference="alignment:Splign:2.1.0"
     exon            749..857
                     /gene="SHANK2"
                     /gene_synonym="AUTS17; CORTBP1; CTTNBP1; ProSAP1; SHANK;
                     SPANK-3"
                     /inference="alignment:Splign:2.1.0"
     exon            858..1009
                     /gene="SHANK2"
                     /gene_synonym="AUTS17; CORTBP1; CTTNBP1; ProSAP1; SHANK;
                     SPANK-3"
                     /inference="alignment:Splign:2.1.0"
     exon            1010..1177
                     /gene="SHANK2"
                     /gene_synonym="AUTS17; CORTBP1; CTTNBP1; ProSAP1; SHANK;
                     SPANK-3"
                     /inference="alignment:Splign:2.1.0"
     exon            1178..1294
                     /gene="SHANK2"
                     /gene_synonym="AUTS17; CORTBP1; CTTNBP1; ProSAP1; SHANK;
                     SPANK-3"
                     /inference="alignment:Splign:2.1.0"
     exon            1295..1372
                     /gene="SHANK2"
                     /gene_synonym="AUTS17; CORTBP1; CTTNBP1; ProSAP1; SHANK;
                     SPANK-3"
                     /inference="alignment:Splign:2.1.0"
     exon            1373..1439
                     /gene="SHANK2"
                     /gene_synonym="AUTS17; CORTBP1; CTTNBP1; ProSAP1; SHANK;
                     SPANK-3"
                     /inference="alignment:Splign:2.1.0"
     exon            1440..1758
                     /gene="SHANK2"
                     /gene_synonym="AUTS17; CORTBP1; CTTNBP1; ProSAP1; SHANK;
                     SPANK-3"
                     /inference="alignment:Splign:2.1.0"
     exon            1759..1928
                     /gene="SHANK2"
                     /gene_synonym="AUTS17; CORTBP1; CTTNBP1; ProSAP1; SHANK;
                     SPANK-3"
                     /inference="alignment:Splign:2.1.0"
     exon            1929..2042
                     /gene="SHANK2"
                     /gene_synonym="AUTS17; CORTBP1; CTTNBP1; ProSAP1; SHANK;
                     SPANK-3"
                     /inference="alignment:Splign:2.1.0"
     exon            2043..2118
                     /gene="SHANK2"
                     /gene_synonym="AUTS17; CORTBP1; CTTNBP1; ProSAP1; SHANK;
                     SPANK-3"
                     /inference="alignment:Splign:2.1.0"
     exon            2119..2201
                     /gene="SHANK2"
                     /gene_synonym="AUTS17; CORTBP1; CTTNBP1; ProSAP1; SHANK;
                     SPANK-3"
                     /inference="alignment:Splign:2.1.0"
     exon            2202..2326
                     /gene="SHANK2"
                     /gene_synonym="AUTS17; CORTBP1; CTTNBP1; ProSAP1; SHANK;
                     SPANK-3"
                     /inference="alignment:Splign:2.1.0"
     exon            2327..2462
                     /gene="SHANK2"
                     /gene_synonym="AUTS17; CORTBP1; CTTNBP1; ProSAP1; SHANK;
                     SPANK-3"
                     /inference="alignment:Splign:2.1.0"
     exon            2463..2543
                     /gene="SHANK2"
                     /gene_synonym="AUTS17; CORTBP1; CTTNBP1; ProSAP1; SHANK;
                     SPANK-3"
                     /inference="alignment:Splign:2.1.0"
     exon            2544..2552
                     /gene="SHANK2"
                     /gene_synonym="AUTS17; CORTBP1; CTTNBP1; ProSAP1; SHANK;
                     SPANK-3"
                     /inference="alignment:Splign:2.1.0"
     exon            2553..2573
                     /gene="SHANK2"
                     /gene_synonym="AUTS17; CORTBP1; CTTNBP1; ProSAP1; SHANK;
                     SPANK-3"
                     /inference="alignment:Splign:2.1.0"
     exon            2574..2704
                     /gene="SHANK2"
                     /gene_synonym="AUTS17; CORTBP1; CTTNBP1; ProSAP1; SHANK;
                     SPANK-3"
                     /inference="alignment:Splign:2.1.0"
     exon            2705..2816
                     /gene="SHANK2"
                     /gene_synonym="AUTS17; CORTBP1; CTTNBP1; ProSAP1; SHANK;
                     SPANK-3"
                     /inference="alignment:Splign:2.1.0"
     exon            2817..2837
                     /gene="SHANK2"
                     /gene_synonym="AUTS17; CORTBP1; CTTNBP1; ProSAP1; SHANK;
                     SPANK-3"
                     /inference="alignment:Splign:2.1.0"
     exon            2838..5244
                     /gene="SHANK2"
                     /gene_synonym="AUTS17; CORTBP1; CTTNBP1; ProSAP1; SHANK;
                     SPANK-3"
                     /inference="alignment:Splign:2.1.0"
     exon            5245..10830
                     /gene="SHANK2"
                     /gene_synonym="AUTS17; CORTBP1; CTTNBP1; ProSAP1; SHANK;
                     SPANK-3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9234..9239
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SHANK2"
                     /gene_synonym="AUTS17; CORTBP1; CTTNBP1; ProSAP1; SHANK;
                     SPANK-3"
                     /note="hexamer: AATATA"
     polyA_site      9251
                     /gene="SHANK2"
                     /gene_synonym="AUTS17; CORTBP1; CTTNBP1; ProSAP1; SHANK;
                     SPANK-3"
                     /note="major polyA site"
     regulatory      10812..10817
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SHANK2"
                     /gene_synonym="AUTS17; CORTBP1; CTTNBP1; ProSAP1; SHANK;
                     SPANK-3"
                     /note="hexamer: AATAAA"
     polyA_site      10830
                     /gene="SHANK2"
                     /gene_synonym="AUTS17; CORTBP1; CTTNBP1; ProSAP1; SHANK;
                     SPANK-3"
ORIGIN      
        1 cttccccacc ccctgcacct gagccgcggc ggcggctcct gagctccggc ggggctgggc
       61 gcggcgccgc tcgggttccc gcggccgatc caggtgtgcg cggacgctgg cgctcccggg
      121 gggctcggac ccgcccccga ctcgccgagc caggtctgga aatctctcca ggcaccctgc
      181 tttggccctc tagaatacag acagttcctg ctctaagagg ccatctgtga ggctcagtct
      241 cccaacacct ctgacacagg cagccatgcc gcgcagccca acatccagcg aggacgagat
      301 ggcccagagc ttctccgact actccgtggg gtcggagtca gacagctcca aagaagagac
      361 catctatgac acgatccggg ccactgcgga gaagccgggc ggtgccagga cggaggagag
      421 ccagggcaac acgctggtga tccgcgtggt catccatgac ctgcagcaga cgaaatgcat
      481 tcgatttaac ccggatgcca cagtgtgggt tgcaaagcag cggatcctgt gtacattaac
      541 ccagagtttg aaagatgtcc tgaactacgg cctgttccag ccggccagca atgggcgtga
      601 cggcaagttc ctggatgagg agcggctcct gcgcgagtac ccacagcccg tgggtgaggg
      661 cgttccttcc ctggagtttc gatacaagaa gcgggtgtat aaacaagcca gtctcgatga
      721 gaaacagttg gccaagctcc acacgaagac caatctgaag aaatgcatgg atcacattca
      781 gcatcgcttg gtggagaaga tcaccaagat gctggaccga ggcctggatc ccaatttcca
      841 cgacccggag accggagaga cccccctgac cttagccgct cagctggacg actctgtgga
      901 ggtcatcaaa gctctcaaaa atggtggagc tcacctggac ttccgtgcca aagatgggat
      961 gaccgcccta cacaaagctg cccgagcgag gaaccaagtt gccctgaaga cccttttaga
     1021 gcttggtgca tccccagatt ataaagacag ttacggcctc accccgctgt atcacacagc
     1081 catcgtcgga ggtgatccct actgctgcga gcttctcctg cacgaacacg ccactgtgtg
     1141 ctgcaaagat gagaacggct ggcacgagat ccaccaggcc tgcaggtacg ggcacgtgca
     1201 gcacctggag cacctgctgt tctacggggc agacatgagt gcccagaatg cctcggggaa
     1261 cacggccttg cacatctgcg ccctctacaa ccaggacagc tgtgcaagag tgcttctgtt
     1321 tcgaggcgga aataaggagt taaaaaacta caacagccag actccatttc aggtggccat
     1381 aatagcaggc aactttgagc tggcagaata catcaagaac cacaaggaaa cagacattgt
     1441 gcccttccga gaggccccgg cgtactccaa ccgccggcgg cggcccccca acacgctggc
     1501 cgccccccgg gtcctgctgc gctccaacag tgacaacaac ctcaatgcca gcgctcccga
     1561 ctgggccgtc tgctccacgg ccacctcgca ccgcagcctg tcaccccagc tgctgcagca
     1621 gatgcccagc aagcccgagg gggccgcgaa gaccattggg agctacgtgc ccgggccccg
     1681 cagccggtcc ccatcgctca acaggctggg cggcgcaggc gaggacggca agaggccgca
     1741 gcctctctgg catgtcgggt cgccttttgc tcttggtgcc aacaaggact cactctcggc
     1801 cttcgagtac ccggggccca agcggaagct ctacagtgcc gtgcccggga ggctcttcgt
     1861 cgctgtcaag ccataccaac cccaagtgga cggcgagatc ccccttcacc gcggtgacag
     1921 ggtcaaagtt ctgagcatcg gtgaaggggg cttctgggaa ggcagcgccc gcggccacat
     1981 cggatggttt ccggcggagt gcgtggagga ggtccagtgt aagcccaggg acagccaggc
     2041 agaaacccgc gctgaccgca gcaagaagct tttcaggcac tacaccgtgg gctcctatga
     2101 cagcttcgac acttccagtg actgcattat tgaggagaag acggtggtcc tgcagaaaaa
     2161 agacaatgag ggctttggat tcgtgcttcg aggggccaaa gctgacacac ccattgaaga
     2221 attcacacca acaccggctt tcccagccct acagtacctg gagtccgtgg atgaaggtgg
     2281 ggtggcgtgg caagccggac taaggaccgg ggacttcttg attgaggtta acaatgagaa
     2341 tgttgtcaaa gtcggccaca ggcaggtggt gaacatgatc cggcagggag ggaatcacct
     2401 ggtccttaag gtggtcacgg tgaccaggaa tctggacccc gacgacaccg ccaggaagaa
     2461 agctcccccg cctccaaagc gggcaccgac cacagccctc accctgcgct ccaagtccat
     2521 gacctcggag ctggaggagc tcgtggataa agcctcggtc cggaagaaga aggataaacc
     2581 cgaggagata gtcccggcct ccaagccctc ccgcgctgct gagaacatgg ctgtggaacc
     2641 gagggtggcg accatcaagc agcggcccag cagccggtgc ttcccggcgg gctcagacat
     2701 gaactctgtg tacgaacgcc aaggaatcgc cgtgatgacg cccactgttc ctgggagccc
     2761 aaaagccccg tttctgggca tccctcgagg tacgatgcga aggcagaaat caatagacag
     2821 cagaatcttt ctatcaggaa taacagagga agagcggcag tttctggctc ctccaatgct
     2881 gaagttcacc agaagcctgt ccatgccgga cacctctgag gacatccccc ctccaccgca
     2941 gtctgtgccc ccgtccccac caccaccttc cccaaccact tacaactgcc ccaagtcccc
     3001 aactccaaga gtctacggga cgattaagcc tgcgttcaat cagaattctg ccgccaaggt
     3061 gtcccccgcc accaggtccg acaccgtggc caccatgatg agggagaagg ggatgtactt
     3121 caggagagag ctggaccgct actccttgga ctctgaagac ctctacagtc ggaatgccgg
     3181 cccgcaagcc aacttccgca acaagagagg ccagatgcca gaaaacccat actcagaggt
     3241 ggggaagatc gccagcaaag ccgtctacgt ccccgccaag cccgccaggc ggaaggggat
     3301 gctggtgaag cagtccaacg tggaggacag ccccgagaag acgtgctcca tccctatccc
     3361 gaccatcatc gtgaaggagc cgtccaccag cagcagcggc aagagcagcc agggcagcag
     3421 catggagatc gacccccagg ccccggagcc accgagccag ctgcggcctg acgaaagcct
     3481 gaccgtcagc agcccctttg ccgccgccat cgccggagcc gtccgcgacc gtgagaagcg
     3541 gctggaagcc aggaggaact ccccggcctt cctctccaca gacctggggg atgaggatgt
     3601 gggcctgggg ccacccgccc ccaggacgcg gccctccatg ttccccgagg agggggattt
     3661 tgctgacgag gacagcgctg agcagctgtc atcccccatg ccgagtgcca cgcccaggga
     3721 gcccgaaaac catttcgtgg gtggcgccga ggccagtgct ccgggtgagg ctgggaggcc
     3781 gctgaattcc acgtccaaag cccaggggcc cgagagcagc ccagcagtgc cctccgcgag
     3841 cagcggcaca gccggccccg ggaattatgt ccacccactc acagggcggc tgcttgatcc
     3901 cagctccccg ctggccctgg cactctccgc aagggaccga gccatgaagg agtctcaaca
     3961 gggacccaaa ggggaggccc ccaaggccga cctcaacaaa cctctttaca ttgataccaa
     4021 aatgcggccc agcctggatg ccggcttccc tacggtcacc aggcagaaca cccggggacc
     4081 cctgaggcgg caggagacgg agaacaagta cgagaccgac ctgggccgag accggaaagg
     4141 cgatgacaag aagaacatgc tgatcgacat catggacacg tcccagcaga agtcggctgg
     4201 cctgctgatg gtgcacaccg tggacgccac taagctggac aacgccctgc aggaagagga
     4261 cgagaaggca gaggtggaga tgaagccaga cagctcgccg tccgaggtgc cagaaggtgt
     4321 ttccgaaacc gaaggtgctt tacagatctc cgctgccccc gagcccacca ccgtgcccgg
     4381 cagaaccatc gtcgcggtgg gctccatgga agaggcggtg attttgccat tccgcatccc
     4441 tcctccccct ctggcatccg tggacttgga tgaggatttt atttttacag agccattgcc
     4501 tcctcccctg gaatttgcaa atagttttga tatccccgat gaccgggcag cttctgtccc
     4561 ggctctctca gacttagtga agcagaagaa aagcgacacc cctcagtccc cttcgttgaa
     4621 ctccagccaa ccaaccaact ctgcagacag caagaagcca gccagtcttt caaactgtct
     4681 gcctgcctca ttcctgccac cccctgaaag ctttgacgcc gtcgccgact ctgggatcga
     4741 ggaggtggac agccggagta gcagcgacca ccacctcgag acgaccagca ctatctccac
     4801 cgtgtctagc atctccaccc tgtcttccga aggtggagag aatgtggaca cctgcacagt
     4861 ctatgcagat gggcaagcat ttatggttga caaaccccca gtacctccta agccaaaaat
     4921 gaagcccatc attcacaaaa gcaatgcact ttatcaagac gcgctcgtgg aagaagatgt
     4981 agatagcttt gttatccccc cgcccgctcc cccgcccccg ccgggcagtg cccagcctgg
     5041 gatggccaag gttctccagc caaggacctc caagttgtgg ggcgacgtca cagagatcaa
     5101 aagcccgatt ctctcaggcc caaaggcaaa cgttattagt gaattgaact ctatcctaca
     5161 gcaaatgaac cgagagaaat tggcaaagcc gggggaagga ctggattcac caatgggagc
     5221 caagtccgcc agcctcgctc caagaagccc ggagatcatg agcaccatct caggtacacg
     5281 gagcacgacg gtcaccttca ctgttcgccc cggcacctcc cagcccatca ccctgcagag
     5341 ccggcccccc gactatgaaa gcaggacctc aggaacaaga cgtgccccaa gccctgtggt
     5401 ctcgccaaca gagatgaaca aagagaccct gcccgccccc ctgtctgctg ccaccgcctc
     5461 tccttctccc gctctctcag atgtctttag ccttccaagc cagccccctt ctggggatct
     5521 atttggcttg aacccagcgg gacgcagtag gtcgccatcc ccctcgatac tgcaacagcc
     5581 aatctcaaat aagcctttta caactaaacc tgtccacctg tggactaaac cagatgtggc
     5641 cgattggctg gaaagtctaa acttgggtga acataaagag gccttcatgg acaatgagat
     5701 cgatggcagt cacttaccaa acctgcagaa ggaggacctc atcgatcttg gggtaactcg
     5761 agtcgggcac agaatgaaca tagaaagggc tttgaaacag ctgctggaca gataaggacg
     5821 gctgctctcc acctcgcaga ctgctcttgt tataagtaga gatgggctcg tgctgaaaca
     5881 tctgaatgcc aagcgaagtc tgtgagcatc aaccccactc catgggtttg tctcctggta
     5941 cccaaagaaa tactgagttg tgtccacaac atggctgggt cttcagaccc ctggctcacc
     6001 atgtgggtgt cttgggcagt ttctatcaca catgggacaa ggggagggag tttttctaac
     6061 atggaaaaag attctcagcc tgccgcccag catgcaggtg gcctcgcttt gccgggtccg
     6121 agaggctccc cgtcaatttt gcacgggatc ctagctcttg taggcagaca ccagtgcact
     6181 ctagatacct cctgagacct ccgtcctctg ctttccgggc agctctcacc accccaggcc
     6241 ccggcatgag gcctttcctc agtcctgtgg cctctcagag gacacctgat gctcacctgc
     6301 ccctctttct cctgcacttg gcttgcagtg agatgctccc agatgcattt gtccagtgcc
     6361 ccatcatggg cctgaaaggc agagaaactt tttcctacac agattctttt ccccatctcc
     6421 tcctgtggtt tgcatccatg gctctttggc catgaggttc ctggcagtgc tgggagtttg
     6481 gatgggatcg tgcccagctt tgcttagctt tctttatttc tgcaaatctg ttagcataat
     6541 tccaaggtgg ccaagcagat gtcacatgga gttagtcaaa gcacaaagtc acgattccac
     6601 aatggagggg agacctggcc aagggagcca gccagcgtgc aactgcccaa gctccaggtc
     6661 tccaggacaa gagcagttgt ctgccatgag cacccatcca ggatggagaa taagggcttc
     6721 tctgcctctc agaattcttt ttaattgaag atgtcttgag ctctgcaaag atcagagcag
     6781 gtgagcatcc actttgacat gaaggacaag aagacgcatg gctcatggcg ggcacatgcg
     6841 gctgccagtg agacagcgtc tcctctggga gctgggcggg cacagcatcc tcagttctgt
     6901 gcccagccaa gggtgagcat ctctgctgag acagtccttt tgctctcgga ggccagggaa
     6961 gatggtactt agaggctttt cccctatcgc tctgggtgtc taggaatccc accagcttgt
     7021 cttaacagta caacagcttc tttgaggacc cagtgggtat ggagtataga cagaacccag
     7081 ggttgagaac agaaggtggg cggcaggatc agagtgaaag cagaggcgtg aggagaggaa
     7141 agcagggagg tctcctgggc tgccaggtca gcctctctgg caaggctttc ttgagccccg
     7201 cccctttctt tccccggagt ccctccaccc cataacaata cctcgaattt ccaaaagagg
     7261 tcaccagatg cacatgggcc gcaaaacaca cagtcaggct tccagcacat tctcccccat
     7321 ttggaggata ctcgaatgtc aggtttttgg ttttattatt atttcagaac tagctcagcc
     7381 catctctaat tataaaacat ggttttgttt tttttttttc ctttttttct tgattaggtc
     7441 tggaacagct ctagaatgaa cacataaaat ttagcaattt aaaatctttc tttactgcaa
     7501 gtttaaatag ttgtacagat agtttataag cacaatattt taagaaaaaa aagtggctgg
     7561 tctactaggc agcctttgtg ccacttcagt gctagaaagt taaagaaaaa aaaacttttg
     7621 tgatttaata atactatttc tgtggaataa ttataaaagt atgacctttt taaatcaacc
     7681 ttatttggat gcatctgaac cagcagagct gtgttatatt ttctatcttt gctagaactt
     7741 cgtcattgaa ggacaatttc ttcaaagtgg ttacaattca taatgcagca gtttctccaa
     7801 aaacaaaaac aaaacacaca ccacacacac gcgcttttcc agtcacacac ccctgatgtt
     7861 ggaaccaagt ttttggacct tctgttccaa aaccttttgc aggtcaatct ttgtatttga
     7921 aatgatccaa tccaacttga agtcaattga atattaaggc gctttacttc cgtgtgcttt
     7981 cagtttttcc atcatgagat gaatgagcat tactctagat aaatttcaag acaggatact
     8041 acaggtggcc tgctgaggct gccccatatt ttagaaaatg taaaaatggt ggtttggcca
     8101 ttaatttgtc ttccatttga tgataccgca aaattccgtg agtccattcc tttggcatgg
     8161 cactttccct gggcctacag ttggtattac ctctgtgctc agtgccaggc aaaacactag
     8221 ctcaaaggag agtcaaggaa accgctggca gacgataacc agtcgaaact cgtgacttcg
     8281 gtttgttgaa ctttggcagc cagttggtga gggccagatg ttattccctt tcttaaagat
     8341 actccaagcc acatgccact aaccacaagc aagctggctg caagactaaa gagctgataa
     8401 catagtttat ttttacactg tcttattata gagaagtaat agacctatca gaacctgcac
     8461 tgaccaacaa ataaacacat gttgccaaga tgaatcggtc tctatctcta tctgcttatt
     8521 ttggtactga aagcaatagt tcctcattca aatcaccacc cactgttctc cccctttggg
     8581 acatgttagg acgaggccct attccatgcc cctctttaat ggtggaacaa atgttaaact
     8641 gctcatctaa agatcatgtt gatattattc caggttttaa gatcaacttt tgttacatac
     8701 tgtaatttaa ataaactgca tttacatgcc tagtttctgt aatattgtgt atacaaaacc
     8761 caaatctctc aaaatgtaaa ttatgtatac ctgccaagat accttttcca gggtgtctgc
     8821 gcacatttta agttaattca cataatataa aaattactca atgtgactgt tgatttgctg
     8881 aactttacat atcacaaagt gaattatttg tgatacttta gttaataaaa tggtaaattt
     8941 ttttctcagt tattgaacaa gcaagcatta tccagttgat ctggcaatga ctttttgtgt
     9001 gtgggccaca atattgattt tcccattaac aatttttttt tgttttttaa atactaatat
     9061 gtttcacact atagtttgtg taacaacacg tgttcgcatt atctatgttg ctgttacttt
     9121 tgtgctttta ttctttttag actttataaa aaaaaaaaaa agctcctgta atttgcactt
     9181 tctcccaatc cttaaatctc ttgtatggca accaaaatta ctgtaaaaaa ataaatatac
     9241 tattgcacta aggttgtggt tctgattgca aacaaacagt gaacactgtc tgaattaaac
     9301 aaaaagctgc ccgacttgca atctaatgta gattatctca ggcattgtgg ccagctctgc
     9361 ctctctaaaa ctgaccagaa aaatctctct catcgagtaa acaggctcct gtcactgagc
     9421 taatctgcct tggttccatt tccttattct caatttatca atggatacgt gcatgttatt
     9481 tcagaattat gcaaaacgtc aaaatctgct tctgtgaccg ctgctatagg cgtggagctg
     9541 aggctcggct tttccttttg ttctgggtgg aagcagcggt gccgcggagg gccagccaga
     9601 tccggaccct tcccttaggg tccagtctcc ccacacccca gcagggtgtc ttctagccat
     9661 aaggccaagg gagtggcaga actgggccgc ctctctggtt gacaagcaaa ccacatgcta
     9721 aggcttggag caagagagaa tttgtgtcta ttggcaaaga actaagccag gaagacatgg
     9781 gccatccctc cgctttaggg aagcatattt taaacctaaa cgttgaactt cttctttggc
     9841 ctcaccagtg aaaacttgtt gtctttagtt cctaaagttt cttctacttt ggcacattcc
     9901 ccagttgagc agcagcctct atgcttccac gttcaggaaa aattccagtc ctcatatctt
     9961 ttgtagttca ccctcaagct ctcccgcttc accatccaat agtttctccc aaaccttggc
    10021 acccccctag actttgcttc caatggtttc ttccagacca cttttcctag atgaatatat
    10081 tcgtttacct tactaggaaa attattggaa gattttttct tttacttgaa attggaggca
    10141 ttttaataac tggcgaactg gaatgtgttt ctgtatttgt agacaaccat gaacccatgc
    10201 aagtaggtga acattccaca gtggctgggt gaccacagca gctgcatgca gacaggactg
    10261 cccgtgcttt gtggggaatc agagaatttc caaacttgtt tctcagactt ccgcagatct
    10321 catcactttg atttctaatc catgctgtat tggtgatttt gtttatcgtt cctgtaactt
    10381 gttctacatt ccacagtctt taccgtttta tgttcaaaat tacaacaatc cctgtccatt
    10441 gattccactc tggaactctt tgttcatgcc aattttgaaa ttttaatacg agccttcaaa
    10501 taaacacaga aaagaaaaaa aaaaaggaaa aaaaataaag tgtgtgcctt gggtgttttg
    10561 aacctgatct gcataaaacc atattgtatt caaatgctgt atctttcatg aaaggataaa
    10621 tatatcttca atgtattaat gtagctgata aaatagttat agtgccttgt tttctgaact
    10681 gagaagccca tatcaatgca caacgtaaag aaaggacggt tcgagctatg aaatgttgag
    10741 gacacctaca cgtgattctg gagcctttct catcataaga ctgtggaaaa tacacagtcg
    10801 tttaagttaa aaataaaaag taaattgaaa
//
