LOCUS       NM_016148               9636 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens SH3 and multiple ankyrin repeat domains 1 (SHANK1),
            mRNA.
ACCESSION   NM_016148 XM_006723233
VERSION     NM_016148.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9636)
  AUTHORS   Qin,Y., Du,Y., Chen,L., Liu,Y., Xu,W., Liu,Y., Li,Y., Leng,J.,
            Wang,Y., Zhang,X.Y., Feng,J., Zhang,F., Jin,L., Qiu,Z., Gong,X. and
            Wang,H.
  TITLE     A recurrent SHANK1 mutation implicated in autism spectrum disorder
            causes autistic-like core behaviors in mice via downregulation of
            mGluR1-IP3R1-calcium signaling
  JOURNAL   Mol Psychiatry 27 (7), 2985-2998 (2022)
   PUBMED   35388181
  REMARK    GeneRIF: A recurrent SHANK1 mutation implicated in autism spectrum
            disorder causes autistic-like core behaviors in mice via
            downregulation of mGluR1-IP3R1-calcium signaling.
            Erratum:[Mol Psychiatry. 2022 Sep;27(9):3922. doi:
            10.1038/s41380-022-01605-8.. PMID: 35538194]
REFERENCE   2  (bases 1 to 9636)
  AUTHORS   Caillet-Saguy,C. and Wolff,N.
  TITLE     PDZ-Containing Proteins Targeted by the ACE2 Receptor
  JOURNAL   Viruses 13 (11), 2281 (2021)
   PUBMED   34835087
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 9636)
  AUTHORS   May,H.J., Jeong,J., Revah-Politi,A., Cohen,J.S., Chassevent,A.,
            Baptista,J., Baugh,E.H., Bier,L., Bottani,A., Carminho A
            Rodrigues,M.T., Conlon,C., Fluss,J., Guipponi,M., Kim,C.A.,
            Matsumoto,N., Person,R., Primiano,M., Rankin,J., Shinawi,M.,
            Smith-Hicks,C., Telegrafi,A., Toy,S., Uchiyama,Y., Aggarwal,V.,
            Goldstein,D.B., Roche,K.W. and Anyane-Yeboa,K.
  TITLE     Truncating variants in the SHANK1 gene are associated with a
            spectrum of neurodevelopmental disorders
  JOURNAL   Genet Med 23 (10), 1912-1921 (2021)
   PUBMED   34113010
  REMARK    GeneRIF: Truncating variants in the SHANK1 gene are associated with
            a spectrum of neurodevelopmental disorders.
REFERENCE   4  (bases 1 to 9636)
  AUTHORS   Kliche,J., Kuss,H., Ali,M. and Ivarsson,Y.
  TITLE     Cytoplasmic short linear motifs in ACE2 and integrin beta3 link
            SARS-CoV-2 host cell receptors to mediators of endocytosis and
            autophagy
  JOURNAL   Sci Signal 14 (665) (2021)
   PUBMED   33436498
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 9636)
  AUTHORS   Fu,Y., Liu,D., Guo,J., Long,H., Xiao,W., Xiao,W., Feng,L., Luo,Z.
            and Xiao,B.
  TITLE     Dynamic Change of Shanks Gene mRNA Expression and DNA Methylation
            in Epileptic Rat Model and Human Patients
  JOURNAL   Mol Neurobiol 57 (9), 3712-3726 (2020)
   PUBMED   32564287
  REMARK    GeneRIF: Dynamic Change of Shanks Gene mRNA Expression and DNA
            Methylation in Epileptic Rat Model and Human Patients.
REFERENCE   6  (bases 1 to 9636)
  AUTHORS   Tobaben,S., Sudhof,T.C. and Stahl,B.
  TITLE     The G protein-coupled receptor CL1 interacts directly with proteins
            of the Shank family
  JOURNAL   J Biol Chem 275 (46), 36204-36210 (2000)
   PUBMED   10958799
REFERENCE   7  (bases 1 to 9636)
  AUTHORS   Sheng,M. and Kim,E.
  TITLE     The Shank family of scaffold proteins
  JOURNAL   J Cell Sci 113 (Pt 11), 1851-1856 (2000)
   PUBMED   10806096
  REMARK    Review article
REFERENCE   8  (bases 1 to 9636)
  AUTHORS   Zitzer,H., Honck,H.H., Bachner,D., Richter,D. and Kreienkamp,H.J.
  TITLE     Somatostatin receptor interacting protein defines a novel family of
            multidomain proteins present in human and rodent brain
  JOURNAL   J Biol Chem 274 (46), 32997-33001 (1999)
   PUBMED   10551867
REFERENCE   9  (bases 1 to 9636)
  AUTHORS   Tu,J.C., Xiao,B., Naisbitt,S., Yuan,J.P., Petralia,R.S.,
            Brakeman,P., Doan,A., Aakalu,V.K., Lanahan,A.A., Sheng,M. and
            Worley,P.F.
  TITLE     Coupling of mGluR/Homer and PSD-95 complexes by the Shank family of
            postsynaptic density proteins
  JOURNAL   Neuron 23 (3), 583-592 (1999)
   PUBMED   10433269
REFERENCE   10 (bases 1 to 9636)
  AUTHORS   Naisbitt,S., Kim,E., Tu,J.C., Xiao,B., Sala,C., Valtschanoff,J.,
            Weinberg,R.J., Worley,P.F. and Sheng,M.
  TITLE     Shank, a novel family of postsynaptic density proteins that binds
            to the NMDA receptor/PSD-95/GKAP complex and cortactin
  JOURNAL   Neuron 23 (3), 569-582 (1999)
   PUBMED   10433268
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC010325.7, EU872208.1, AF163302.1 and AC008743.9.
            
            On Jun 2, 2019 this sequence version replaced NM_016148.4.
            
            Summary: This gene encodes a member of the SHANK (SH3 domain and
            ankyrin repeat containing) family of proteins. Members of this
            family act as scaffold proteins that are required for the
            development and function of neuronal synapses. Deletions in this
            gene may be associated with autism spectrum disorder in males.
            [provided by RefSeq, Apr 2016].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments and RNA-seq
            data.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            CDS exon combination :: AF163302.1 [ECO:0000331]
            RNAseq introns       :: mixed sample support SAMEA1965299,
                                    SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            MANE Ensembl match        :: ENST00000293441.6/ ENSP00000293441.1
            RefSeq Select criteria    :: based on manual assertion,
                                         conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-19                AC010325.7         52449-52467         c
            20-315              EU872208.1         1-296
            316-421             EU872208.1         298-403
            422-3114            AF163302.1         1-2693
            3115-6208           AC008743.9         165838-168931       c
            6209-7055           AF163302.1         5788-6634
            7056-9636           AC008743.9         158901-161481       c
FEATURES             Location/Qualifiers
     source          1..9636
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.33"
     gene            1..9636
                     /gene="SHANK1"
                     /gene_synonym="SPANK-1; SSTRIP; synamon"
                     /note="SH3 and multiple ankyrin repeat domains 1"
                     /db_xref="GeneID:50944"
                     /db_xref="HGNC:HGNC:15474"
                     /db_xref="MIM:604999"
     exon            1..397
                     /gene="SHANK1"
                     /gene_synonym="SPANK-1; SSTRIP; synamon"
                     /inference="alignment:Splign:2.1.0"
     exon            398..695
                     /gene="SHANK1"
                     /gene_synonym="SPANK-1; SSTRIP; synamon"
                     /inference="alignment:Splign:2.1.0"
     CDS             441..6926
                     /gene="SHANK1"
                     /gene_synonym="SPANK-1; SSTRIP; synamon"
                     /note="somatostatin receptor-interacting protein;
                     SSTR-interacting protein"
                     /codon_start=1
                     /product="SH3 and multiple ankyrin repeat domains protein
                     1"
                     /protein_id="NP_057232.2"
                     /db_xref="CCDS:CCDS12799.1"
                     /db_xref="GeneID:50944"
                     /db_xref="HGNC:HGNC:15474"
                     /db_xref="MIM:604999"
                     /translation="MTHSPATSEDEERHSASECPEGGSESDSSPDGPGRGPRGTRGQG
                     SGAPGSLASVRGLQGRSMSVPDDAHFSMMVFRIGIPDLHQTKCLRFNPDATIWTAKQQ
                     VLCALSESLQDVLNYGLFQPATSGRDANFLEEERLLREYPQSFEKGVPYLEFRYKTRV
                     YKQTNLDEKQLAKLHTKTGLKKFLEYVQLGTSDKVARLLDKGLDPNYHDSDSGETPLT
                     LAAQTEGSVEVIRTLCLGGAHIDFRARDGMTALHKAACARHCLALTALLDLGGSPNYK
                     DRRGLTPLFHTAMVGGDPRCCELLLFNRAQLGIADENGWQEIHQACQRGHSQHLEHLL
                     FYGAEPGAQNASGNTALHICALYNKETCARILLYRGADKDVKNNNGQTPFQVAVIAGN
                     FELGELIRNHREQDVVPFQESPKYAARRRGPPGTGLTVPPALLRANSDTSMALPDWMV
                     FSAPGAASSGAPGPTSGSQGQSQPSAPTTKLSSGTLRSASSPRGARARSPSRGRHPED
                     AKRQPRGRPSSSGTPREGPAGGTGGSGGPGGSLGSRGRRRKLYSAVPGRSFMAVKSYQ
                     AQAEGEISLSKGEKIKVLSIGEGGFWEGQVKGRVGWFPSDCLEEVANRSQESKQESRS
                     DKAKRLFRHYTVGSYDSFDAPSLMDGIGPGSDYIIKEKTVLLQKKDSEGFGFVLRGAK
                     AQTPIEEFTPTPAFPALQYLESVDEGGVAWRAGLRMGDFLIEVNGQNVVKVGHRQVVN
                     MIRQGGNTLMVKVVMVTRHPDMDEAVHKKAPQQAKRLPPPTISLRSKSMTSELEEMEY
                     EQQPAPVPSMEKKRTVYQMALNKLDEILAAAQQTISASESPGPGGLASLGKHRPKGFF
                     ATESSFDPHHRAQPSYERPSFLPPGPGLMLRQKSIGAAEDDRPYLAPPAMKFSRSLSV
                     PGSEDIPPPPTTSPPEPPYSTPPVPSSSGRLTPSPRGGPFNPGSGGPLPASSPASFDG
                     PSPPDTRVGSREKSLYHSGPLPPAHHHPPHHHHHHAPPPQPHHHHAHPPHPPEMETGG
                     SPDDPPPRLALGPQPSLRGWRGGGPSPTPGAPSPSHHGSAGGGGGSSQGPALRYFQLP
                     PRAASAAMYVPARSGRGRKGPLVKQTKVEGEPQKGGGLPPAPSPTSPASPQPPPAVAA
                     PSEKNSIPIPTIIIKAPSTSSSGRSSQGSSTEAEPPTQPEPTGGGGGGGSSPSPAPAM
                     SPVPPSPSPVPTPASPSGPATLDFTSQFGAALVGAARREGGWQNEARRRSTLFLSTDA
                     GDEDGGDGGLGTGAAPGPRLRHSKSIDEGMFSAEPYLRLESAGSGAGYGGYGAGSRAY
                     GGGGGSSAFTSFLPPRPLVHPLTGKALDPASPLGLALAARERALKESSEGGGAPQPPP
                     RPPSPRYEAPPPTPHHHSPHAHHEPVLRLWGASPPDPARRELGYRAGLGSQEKSLPAS
                     PPAARRSLLHRLPPTAPGVGPLLLQLGTEPPAPHPGVSKPWRSAAPEEPERLPLHVRF
                     LENCQPRAPVTSGRGPPSEDGPGVPPPSPRRSVPPSPTSPRASEENGLPLLVLPPPAP
                     SVDVEDGEFLFVEPLPPPLEFSNSFEKPESPLTPGPPHPLPDTPAPATPLPPVPPPAV
                     AAAPPTLDSTASSLTSYDSEVATLTQGASAAPGDPHPPGPPAPAAPAPAAPQPGPDPP
                     PGTDSGIEEVDSRSSSDHPLETISSASTLSSLSAEGGGSAGGGGGAGAGVASGPELLD
                     TYVAYLDGQAFGGSSTPGPPYPPQLMTPSKLRGRALGASGGLRPGPSGGLRDPVTPTS
                     PTVSVTGAGTDGLLALRACSGPPTAGVAGGPVAVEPEVPPVPLPTASSLPRKLLPWEE
                     GPGPPPPPLPGPLAQPQASALATVKASIISELSSKLQQFGGSSAAGGALPWARGGSGG
                     GGDSHHGGASYVPERTSSLQRQRLSDDSQSSLLSKPVSSLFQNWPKPPLPPLPTGTGV
                     SPTAAAAPGATSPSASSSSTSTRHLQGVEFEMRPPLLRRAPSPSLLPASEHKVSPAPR
                     PSSLPILPSGPLYPGLFDIRGSPTGGAGGSADPFAPVFVPPHPGISGGLGGALSGASR
                     SLSPTRLLSLPPDKPFGAKPLGFWTKFDVADWLEWLGLAEHRAQFLDHEIDGSHLPAL
                     TKEDYVDLGVTRVGHRMNIDRALKFFLER"
     exon            696..899
                     /gene="SHANK1"
                     /gene_synonym="SPANK-1; SSTRIP; synamon"
                     /inference="alignment:Splign:2.1.0"
     exon            900..971
                     /gene="SHANK1"
                     /gene_synonym="SPANK-1; SSTRIP; synamon"
                     /inference="alignment:Splign:2.1.0"
     exon            972..1080
                     /gene="SHANK1"
                     /gene_synonym="SPANK-1; SSTRIP; synamon"
                     /inference="alignment:Splign:2.1.0"
     exon            1081..1232
                     /gene="SHANK1"
                     /gene_synonym="SPANK-1; SSTRIP; synamon"
                     /inference="alignment:Splign:2.1.0"
     exon            1233..1400
                     /gene="SHANK1"
                     /gene_synonym="SPANK-1; SSTRIP; synamon"
                     /inference="alignment:Splign:2.1.0"
     exon            1401..1517
                     /gene="SHANK1"
                     /gene_synonym="SPANK-1; SSTRIP; synamon"
                     /inference="alignment:Splign:2.1.0"
     exon            1518..1595
                     /gene="SHANK1"
                     /gene_synonym="SPANK-1; SSTRIP; synamon"
                     /inference="alignment:Splign:2.1.0"
     exon            1596..1662
                     /gene="SHANK1"
                     /gene_synonym="SPANK-1; SSTRIP; synamon"
                     /inference="alignment:Splign:2.1.0"
     exon            1663..1993
                     /gene="SHANK1"
                     /gene_synonym="SPANK-1; SSTRIP; synamon"
                     /inference="alignment:Splign:2.1.0"
     exon            1994..2187
                     /gene="SHANK1"
                     /gene_synonym="SPANK-1; SSTRIP; synamon"
                     /inference="alignment:Splign:2.1.0"
     exon            2188..2301
                     /gene="SHANK1"
                     /gene_synonym="SPANK-1; SSTRIP; synamon"
                     /inference="alignment:Splign:2.1.0"
     exon            2302..2377
                     /gene="SHANK1"
                     /gene_synonym="SPANK-1; SSTRIP; synamon"
                     /inference="alignment:Splign:2.1.0"
     exon            2378..2404
                     /gene="SHANK1"
                     /gene_synonym="SPANK-1; SSTRIP; synamon"
                     /inference="alignment:Splign:2.1.0"
     exon            2405..2487
                     /gene="SHANK1"
                     /gene_synonym="SPANK-1; SSTRIP; synamon"
                     /inference="alignment:Splign:2.1.0"
     exon            2488..2612
                     /gene="SHANK1"
                     /gene_synonym="SPANK-1; SSTRIP; synamon"
                     /inference="alignment:Splign:2.1.0"
     exon            2613..2748
                     /gene="SHANK1"
                     /gene_synonym="SPANK-1; SSTRIP; synamon"
                     /inference="alignment:Splign:2.1.0"
     exon            2749..2829
                     /gene="SHANK1"
                     /gene_synonym="SPANK-1; SSTRIP; synamon"
                     /inference="alignment:Splign:2.1.0"
     exon            2830..2898
                     /gene="SHANK1"
                     /gene_synonym="SPANK-1; SSTRIP; synamon"
                     /inference="alignment:Splign:2.1.0"
     exon            2899..3017
                     /gene="SHANK1"
                     /gene_synonym="SPANK-1; SSTRIP; synamon"
                     /inference="alignment:Splign:2.1.0"
     exon            3018..3114
                     /gene="SHANK1"
                     /gene_synonym="SPANK-1; SSTRIP; synamon"
                     /inference="alignment:Splign:2.1.0"
     exon            3115..6208
                     /gene="SHANK1"
                     /gene_synonym="SPANK-1; SSTRIP; synamon"
                     /inference="alignment:Splign:2.1.0"
     exon            6209..9636
                     /gene="SHANK1"
                     /gene_synonym="SPANK-1; SSTRIP; synamon"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9609..9614
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SHANK1"
                     /gene_synonym="SPANK-1; SSTRIP; synamon"
                     /note="hexamer: ATTAAA"
     polyA_site      9636
                     /gene="SHANK1"
                     /gene_synonym="SPANK-1; SSTRIP; synamon"
                     /note="major polyA site"
ORIGIN      
        1 agagacagcg agagcgagag agcgagaagg gtggcagagg aggcgcggag cgggcggcgg
       61 cggcggcggc ccgaggaaga ggaggaggaa gagcaggcgg agacgcggcg cccggagccg
      121 gccggaccga gccgggggcg ggcgaggagg ggcgcggcgg ccgcggcgga agggggggcg
      181 ctctgcggat ggccaggccc ggcccgcggg gggggtgagg tcctcggccc tcccgccctc
      241 ccccccggtc cccgcccgcc ccctccccgg gcccgctcgc cctccggggc ggtggggcat
      301 ggcctgaggg tccccgtctc cgggggggtg gggggtgggg ggagggggga ggggccgcgg
      361 gcgccgccga ccgggactca gcagccccgc caggcagccg cctgtgatgc tgccgtcccc
      421 cgtcgccccg tggccccaca atgacccaca gccccgcgac aagcgaggac gaggaacgcc
      481 acagtgccag cgagtgtccc gaggggggct cagagtccga cagctcccca gacgggccag
      541 gtcgaggccc ccgggggacc cggggccagg gcagtggggc acctggtagc ctggcctctg
      601 ttagaggcct ccagggccgc tcaatgtccg tcccagacga cgcccacttc agcatgatgg
      661 tcttcaggat tggcatcccg gacctgcacc agacaaaatg ccttcgcttc aaccccgatg
      721 ccaccatctg gacggccaag cagcaggtgc tctgtgccct gagcgagagc ctgcaggatg
      781 tgctcaacta tggcctgttc caaccggcca cctccggccg cgatgccaac ttcctggagg
      841 aggagaggct gctgcgggag tacccccagt cctttgagaa gggggtcccc tacctggagt
      901 tccgatacaa gacccgagtt tacaaacaga ccaacctgga tgagaagcag ctggccaagt
      961 tgcacacgaa gacggggttg aagaagttcc tggagtatgt gcagctcggg acatctgaca
     1021 aggtggcgcg gctgctggac aaggggctgg accccaatta ccatgactcg gattcgggag
     1081 agaccccctt gacactggcg gcccagaccg aaggctctgt agaggtgatt cgaaccctgt
     1141 gcctgggcgg ggcccacatt gacttccggg cccgggatgg catgaccgca ctgcataagg
     1201 ccgcatgcgc ccgacactgc ctggcactca cggcgctcct ggaccttggg ggttccccca
     1261 actacaagga ccgtcggggg ctgacccctc tgttccacac ggccatggtg ggtggtgacc
     1321 cccgatgctg cgagctgctc ctgttcaaca gggcccagct gggcatagct gatgagaacg
     1381 gctggcagga aatccaccag gcctgccagc ggggtcactc tcagcacctg gagcatctgc
     1441 ttttctacgg ggctgagcct ggagcccaga acgcctcggg gaacacggct ctgcacatct
     1501 gcgccctcta caacaaggag acctgtgcca ggatcctcct gtatcgaggt gccgacaagg
     1561 atgtgaagaa caacaacgga cagaccccct tccaggtggc agtgattgct gggaattttg
     1621 agctggggga gctgatccga aaccaccgag aacaggatgt ggtgcccttc caggagtccc
     1681 ccaagtacgc ggcccggcga cgggggcccc caggcacagg gctgacggtg cccccggcgc
     1741 tgctgcgggc caacagtgac accagcatgg cgctgcccga ctggatggtg ttctccgccc
     1801 cgggggccgc gtcctctggg gcccctggcc ctacctcagg gtcccagggc cagtcgcagc
     1861 cctcggcccc caccaccaag ctcagcagcg ggaccctccg aagtgccagc agcccccggg
     1921 gtgccagggc ccgctctcca tcccgaggga ggcaccctga ggacgccaag aggcagcccc
     1981 gaggccggcc cagctccagc gggacacccc gggaagggcc agccgggggc acggggggct
     2041 cagggggccc cgggggctcc ctgggcagcc gcgggaggcg gaggaagctc tactcagcgg
     2101 tacccggacg ctccttcatg gctgtgaagt cctaccaggc ccaagccgag ggggagatct
     2161 ccctgagcaa gggcgagaag atcaaagtac ttagcatcgg ggaaggaggc ttctgggaag
     2221 gccaggtcaa aggtcgtgtt ggctggttcc cctctgactg cctggaagaa gtggcgaatc
     2281 gctctcagga gagcaagcaa gaaagccgca gtgacaaggc aaagagactc ttccggcatt
     2341 ataccgtggg ctcctacgac agctttgatg ccccaagctt aatggatggg attggcccag
     2401 ggagcgatta catcattaag gagaagacag tcttgctgca gaagaaggac agtgaggggt
     2461 ttgggttcgt gctccggggg gccaaggcgc agacccccat cgaggagttc acccccaccc
     2521 cggccttccc ggcgctgcag tacctggagt cggtggacga gggtggcgtg gcatggcgag
     2581 ctggactgcg aatgggagac ttcctcatcg aggtgaacgg gcagaatgtg gtgaaggtcg
     2641 gccaccgaca ggtggtgaac atgatccgcc aagggggcaa cacgctgatg gtgaaggtgg
     2701 tgatggtcac caggcacccg gacatggatg aggcagtgca caagaaagca ccccagcagg
     2761 ccaagcggct gccgccccca accatctccc tgcgttccaa atctatgacc tcagagctgg
     2821 aggagatgga gtacgagcag cagccggcgc cggtgcccag catggagaaa aagcggaccg
     2881 tgtatcagat ggctctcaac aaactggacg aaatcctggc cgcagctcaa cagaccatca
     2941 gtgcaagcga aagccctggt cccggtggcc tcgcgtccct gggcaaacac cgacccaaag
     3001 gtttctttgc cactgagtcg agcttcgatc cccaccaccg tgcccagcca agttacgagc
     3061 gtccttcttt cctgcctcca ggacctgggt tgatgctccg gcaaaaatct atcggtgcgg
     3121 cagaagatga cagaccttac ctagcacccc cagccatgaa attcagccgc agcctgtctg
     3181 tgcctggttc ggaggacatt cccccgccac ccaccacgtc cccaccggag cctccctaca
     3241 gcacacctcc agtcccctcc tcctcagggc gcctcacccc ctcccctcgg ggagggccct
     3301 tcaaccctgg ctctggtggc cccctccccg cctcctcccc tgcatccttt gacgggccct
     3361 cccctcccga cactcgcgtg gggagccgcg agaagagcct gtaccacagt gggcccctgc
     3421 ccccggccca ccaccacccg ccccaccacc accaccacca cgccccgccc cctcagcccc
     3481 accaccacca cgcccacccc cctcatcctc ccgagatgga gacaggcggc tctcccgacg
     3541 accctccacc ccgcctggct ctggggcccc agcccagcct gcgaggctgg aggggcggcg
     3601 ggcccagccc gaccccgggg gccccgtccc catcgcacca cggcagcgcg ggcgggggcg
     3661 gcggctcctc ccagggcccg gctctacgct atttccagct gcccccgcgg gcggccagcg
     3721 cagccatgta cgtgcccgcc cgctcgggcc gcggccgcaa gggcccgctg gtcaagcaga
     3781 ccaaggtgga aggcgagccc cagaagggcg gcggcctccc gcccgcgccg tcgcccacgt
     3841 ccccggcctc cccgcagccg ccgcccgccg tggccgcgcc ctcggagaag aactccatcc
     3901 ccatccccac catcatcatc aaggccccgt ccaccagtag cagcggccgc agcagccagg
     3961 gcagcagcac cgaggcggag ccccccaccc agccggagcc cacgggaggc ggcggcggcg
     4021 gcggctcctc gcccagcccc gccccggcca tgtcacccgt gcccccgtcc ccctcgcccg
     4081 tgcccacccc cgcctcgccc agcggcccgg ccacgctgga cttcacgagc cagttcgggg
     4141 ccgccctggt gggggcggcc cggagggagg ggggctggca gaatgaggcg cgccggcgct
     4201 ccacgctgtt cctgtccacc gacgcggggg acgaggacgg cggggacggc gggctgggca
     4261 caggggcggc cccgggcccg cggctgcgcc actccaaatc catcgacgag ggcatgttct
     4321 ccgccgagcc ctacctccga ctggagtctg cgggcagcgg cgcgggctac ggcggctacg
     4381 gggccggtag ccgagcctac gggggtggcg ggggcagcag cgccttcacc agcttcctgc
     4441 ccccgcgacc cctggtgcac ccgctgaccg gcaaggccct ggatcccgcc tccccgctgg
     4501 ggctggccct ggccgcccgc gagcgagcgc tgaaggagtc ctcggagggc ggcggggccc
     4561 cccagccgcc tcccaggccc ccatcgcccc gctacgaggc cccgccgccc accccgcacc
     4621 accactcgcc ccacgcccac cacgagccag tgctgcgtct ctggggggcc tccccgccgg
     4681 accctgcgcg ccgggagctg gggtacaggg ccgggctggg cagccaggag aagtcccttc
     4741 ccgccagccc gcccgccgcc cggcgttccc tgctacaccg cctgccgccc accgctcccg
     4801 gggtggggcc cctcctgctg cagctgggga cggagccccc ggccccgcac cccggagtaa
     4861 gcaagccctg gaggtccgca gcccccgaag aacccgagcg gctgccgctg cacgtgcggt
     4921 tccttgaaaa ctgccagccc cgggcccctg tgacgagcgg aaggggtccc ccctcggagg
     4981 acgggccggg ggtcccgccg cccagcccac gccggtccgt gcccccctcc ccgacctccc
     5041 cgagggccag cgaagagaac gggctgcccc tgctggtcct gccgcctccc gccccctcgg
     5101 tggatgtgga agatggcgaa ttccttttcg tggaaccgct gcctccgcct ctggaattct
     5161 ccaacagctt cgaaaagcca gagtcgcccc tcacgcctgg gcctccccac ccgctgcccg
     5221 acacacctgc ccctgccacc ccgttacccc ctgtgccacc cccggctgtg gccgcagccc
     5281 ctcccaccct ggactccacc gcatccagcc tgacatccta tgacagcgag gtggccaccc
     5341 tgacccaggg ggcctccgcc gctcctgggg acccccatcc accaggcccg cctgccccag
     5401 cagcaccggc tcccgctgcc ccacagcctg gcccggaccc tccgcctggc acggattctg
     5461 gcatcgagga ggtggacagt cggagcagca gtgaccaccc actggagacc atcagcagcg
     5521 cctccacgct gagcagccta tctgccgaag gtggtggcag cgcagggggt gggggcgggg
     5581 ctggggccgg tgtggccagt gggccggagc ttctggacac ctatgtggcc tacctggacg
     5641 gccaggcctt tgggggcagc agtactcccg gcccgccata ccctcctcag ctcatgactc
     5701 cctctaagct ccggggccgg gcgctaggag ccagcggagg cctgcggcct ggccccagcg
     5761 ggggactccg agaccctgtt acccccacca gccccaccgt ctcggtgaca ggggctggaa
     5821 ccgatgggct gctggccctg cgtgcttgtt caggaccccc cacggcaggc gtggcggggg
     5881 gtccggtggc tgtagagcca gaagtcccac cggtgccctt gccgacggcc tcctctctgc
     5941 cccggaagct gctgccctgg gaggagggcc cgggcccacc gccaccacct ctgcccgggc
     6001 ccttggccca gcctcaggcc tcagccttgg ccacagtaaa agccagcatc atcagtgaac
     6061 tcagctccaa gcttcagcag tttgggggct cctcggcagc tggcggcgct ctgccctggg
     6121 cccgaggagg cagtggggga ggcggagaca gccaccacgg gggagccagc tatgtccccg
     6181 agaggacctc ctccctgcag cggcagagac tctccgacga ctcccagtcc tcactcctct
     6241 ccaagcctgt cagcagcctg tttcagaact ggcccaaacc acctctgccg ccactcccca
     6301 ccggaacagg ggtctcccct acagccgctg cggccccagg ggccacctca ccctcagcct
     6361 cctcctcctc cacgtccacc cgccacctcc agggcgtgga gttcgagatg cggccccctc
     6421 tgctccgccg ggcccccagc ccctcgctgc tgcccgcctc ggagcacaag gtcagccctg
     6481 cgcccaggcc ctcgtccctg cccatcctgc cttccggacc cctctaccca ggcctctttg
     6541 acatccgtgg ctccccaact ggaggggcag gaggctcggc tgaccccttc gccccagtct
     6601 ttgtgccgcc acacccgggg atatccgggg ggctcggggg agccttgtca ggggcctcgc
     6661 gctccctctc accgacccgc ctgctctcgc tgcccccgga caagccgttt ggcgctaaac
     6721 ctctggggtt ctggaccaag ttcgacgtgg ctgattggct ggagtggctg ggtttggcgg
     6781 agcaccgagc ccagttcctg gaccacgaga tcgatggctc ccacctgccc gccttgacca
     6841 aggaggacta cgtcgatcta ggtgtgacca gggtgggcca ccgcatgaac atcgaccggg
     6901 ctctcaaatt cttcctggag aggtgatggc tggcctggac ggaccagccc cgtccacaga
     6961 actcttgagc ctgctggcct cttgacctct gacccctgac tgtcattctc tccccgggcc
     7021 agggactctg ttcaaactgc gccctgccct catctcccaa ggccagaggt caccaggtgg
     7081 cccaatccag gccggacatt tgcacctcac tgaagggggg cagctgacac aagagtgtgt
     7141 gtgtgtgtgt gaagcgggga gcggaggggg gataggagaa attacagagg ggaattgaga
     7201 aggagggaag ggcctattct ggggggaggg gatagacaga gccatagagg gtcagccctg
     7261 agcccgattg gatggggact gtcccccagg ccgcagggga agggggtgcc ctcggattcc
     7321 accacctgcc acccctctaa caacccttcc cactctctcc aggcccctca gctctccccc
     7381 cttcccctct ccccagcaca cacagctgcc cccctcagcc tcttgcacgc tccgttgcac
     7441 gcctgctcac ctctctcccc tcccgtcccc gggctgcaat tttgcacaaa tttgccctct
     7501 cgctgtcttg cacattctgc ctttgcttct ggctggcgag gcccgctcaa atacactctg
     7561 ctctcctcgc acacgctgca tttcctttct cacgctccat gcctccttct ctgcttcttg
     7621 ctgcttacac acttttcccc taccagggcc ctcctttaca cactccattt cacatctcct
     7681 cctccccgct cgctctggac ccttgttgca gacacacttg ggacacattt tccctccccc
     7741 tcacagactc catgcacatc attcattctc tggagtgccc tgctctttgc tcagcccttg
     7801 cacacttgct atcttatgca cccctcctga cacactctta cctccatttt cttacgttga
     7861 agcactttcc cttcttattc ttaaaaaccc cagattcttc cactccccaa ctctcccctc
     7921 tcaccatgac actcctagcc ccccaccccc cgccttccct cttgcccctt ttgcaccgtc
     7981 acttttggtc acgccttttg cacacttgcc tcccagaaac tgtttctctc acacatgctc
     8041 attctcttct tctttgccag gctcccactc ccttgcccct ttctgtttga aatctttgcc
     8101 cttctgcatt tttgattctt ttgtgaaccc tcttgcacac ttgcttttgc acacgcgccc
     8161 ccccccccag cattttcagt gctctccgga agccttgttt ctccccttta ataatgttct
     8221 tgcgcaccat gacatggctg cacaccgcat cttgtctttt ctacctggat gagagctcta
     8281 ccacttcttt ccttttgcac actcatctca ttccatgcac tcttgggctg taactgtcat
     8341 ttcttttcct gacacgctta ttttttattc ctgttgacaa gcagctcttg cggccattct
     8401 cattcggatt ctcttacctc cttaagctct ttctcttcct ggtgagagaa gaccctatgc
     8461 acactggctt cctgctcacc atgcttccac actcgagcct ctccctgcct ccagccctca
     8521 cccttcctca catcctccct cttctgtctt tcctgggcca tctgtccatc ccttttgcac
     8581 gaagattgcc ctctcctaac ctgtccactc caagaagctc cctctccctc cagcatggct
     8641 gttccttccc gcacgcttcc tctctccgct tattcccggg gcttgggaat gttaagccac
     8701 ccctccccct gaaaagcccc ctcccctgct gtgggtaaag gggggagggc gttgccctca
     8761 ggtccccccc ccatgttcag ctgccaaaga agggagctcc ccctccttcc ccaagcccat
     8821 tccccctctg ctgcccccta cccccgagag gggggggcgc tccgtccatg aggggagggg
     8881 gctgcaagcc ccctcccctc accatgtcag ggatctgcgg ggaacctcgt gggagggtcg
     8941 tgggaggggg gaggggtggt gagagagggg cagaggccgc gctggacccc tacccgcccc
     9001 ctcccccttg tcggatgccc ccagtccgca gggggcctgc gcggcgcccg tctatcaagg
     9061 gctcgttcat tctggatggg tgccgcgggg ttggggaggg tgtagggggt ggggggggga
     9121 ggagaggtcg gggtggggag tggggaggga ccgaatgcgg ggacagcgcg gggtggagag
     9181 gagagaaaaa gcaaaaagag gaacaataac aaaagaagaa aatataaaaa acagaaaaaa
     9241 aaaacacaaa acccctagaa cacacaaaaa aatccagaaa aaaaacctga aataaaagcc
     9301 cgagaattgt cttccaaggg ggggtgggag gggaagagga ggggggcgga tgaggacgtc
     9361 tgattctcgc tgagacaggg ctcggcacgc gctgaaagcc ccggtgccca ggtcttccct
     9421 ttcgcgggct tcagttcatc cctcaaggga ttgggaccca ggcgtccacg tacagggact
     9481 ggaggaagat tagggacgcg ggattataac atgggggttc attcctaccc cccatcacct
     9541 cccttcccct gactccccac caagattttg cagagagaaa tggcttttgt accaggtcat
     9601 tctttataat taaactcaca gtatccaccc cctcga
//
