LOCUS       NM_020145               3013 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens SH3 domain containing GRB2 like, endophilin B2
            (SH3GLB2), transcript variant 2, mRNA.
ACCESSION   NM_020145
VERSION     NM_020145.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 3013)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   4  (bases 1 to 3013)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 3013)
  AUTHORS   Serfass,J.M., Takahashi,Y., Zhou,Z., Kawasawa,Y.I., Liu,Y.,
            Tsotakos,N., Young,M.M., Tang,Z., Yang,L., Atkinson,J.M.,
            Chroneos,Z.C. and Wang,H.G.
  TITLE     Endophilin B2 facilitates endosome maturation in response to growth
            factor stimulation, autophagy induction, and influenza A virus
            infection
  JOURNAL   J Biol Chem 292 (24), 10097-10111 (2017)
   PUBMED   28455444
  REMARK    GeneRIF: Data (including data from studies in knockout mice)
            suggest that SH3GLB2 regulates trafficking of endocytic vesicles
            and autophagosomes to late endosomes or lysosomes; despite having
            an intracellular localization and tissue distribution similar to
            endophilin B1, endophilin B2 is dispensable for mitochondrial
            apoptosis.
REFERENCE   6  (bases 1 to 3013)
  AUTHORS   Behrends,C., Sowa,M.E., Gygi,S.P. and Harper,J.W.
  TITLE     Network organization of the human autophagy system
  JOURNAL   Nature 466 (7302), 68-76 (2010)
   PUBMED   20562859
REFERENCE   7  (bases 1 to 3013)
  AUTHORS   Fasso,M., Waitz,R., Hou,Y., Rim,T., Greenberg,N.M., Shastri,N.,
            Fong,L. and Allison,J.P.
  TITLE     SPAS-1 (stimulator of prostatic adenocarcinoma-specific T
            cells)/SH3GLB2: A prostate tumor antigen identified by CTLA-4
            blockade
  JOURNAL   Proc Natl Acad Sci U S A 105 (9), 3509-3514 (2008)
   PUBMED   18303116
  REMARK    GeneRIF: identified a nonmutated HLA-A2-binding epitope in the
            human ortholog SH3GLB2, which primed T cells from healthy HLA-A2(+)
            individuals
REFERENCE   8  (bases 1 to 3013)
  AUTHORS   Liang,Z.D., Lippman,S.M., Wu,T.T., Lotan,R. and Xu,X.C.
  TITLE     RRIG1 mediates effects of retinoic acid receptor beta2 on tumor
            cell growth and gene expression through binding to and inhibition
            of RhoA
  JOURNAL   Cancer Res 66 (14), 7111-7118 (2006)
   PUBMED   16849557
REFERENCE   9  (bases 1 to 3013)
  AUTHORS   Li,J., Hawkins,I.C., Harvey,C.D., Jennings,J.L., Link,A.J. and
            Patton,J.G.
  TITLE     Regulation of alternative splicing by SRrp86 and its interacting
            proteins
  JOURNAL   Mol Cell Biol 23 (21), 7437-7447 (2003)
   PUBMED   14559993
REFERENCE   10 (bases 1 to 3013)
  AUTHORS   Pierrat,B., Simonen,M., Cueto,M., Mestan,J., Ferrigno,P. and
            Heim,J.
  TITLE     SH3GLB, a new endophilin-related protein family featuring an SH3
            domain
  JOURNAL   Genomics 71 (2), 222-234 (2001)
   PUBMED   11161816
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BM808895.1, BC014635.1, AB058751.2, BC005843.2 and AL592211.27.
            
            On Feb 27, 2020 this sequence version replaced NM_020145.3.
            
            Transcript Variant: This variant (2) lacks two alternate in-frame
            exons in the central coding region, compared to variant 1,
            resulting in an isoform (b) that is shorter than isoform a. Both
            variants 2 and 3 encode isoform b.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC014635.1, SRR12921934.3231524.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000372564.8/ ENSP00000361645.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-80                BM808895.1         34-113
            81-1136             BC014635.1         50-1105
            1137-2010           AB058751.2         1112-1985
            2011-2257           BC005843.2         2182-2428
            2258-3013           AL592211.27        12779-13534         c
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q34.11"
     gene            1..3013
                     /gene="SH3GLB2"
                     /gene_synonym="PP6569; PP9455; RRIG1"
                     /note="SH3 domain containing GRB2 like, endophilin B2"
                     /db_xref="GeneID:56904"
                     /db_xref="HGNC:HGNC:10834"
                     /db_xref="MIM:609288"
     exon            1..240
                     /gene="SH3GLB2"
                     /gene_synonym="PP6569; PP9455; RRIG1"
                     /inference="alignment:Splign:2.1.0"
     CDS             178..1365
                     /gene="SH3GLB2"
                     /gene_synonym="PP6569; PP9455; RRIG1"
                     /note="isoform b is encoded by transcript variant 2;
                     SH3-containing protein SH3GLB2; SH3-domain GRB2-like
                     endophilin B2; endophilin-B2; SH3 domain-containing
                     GRB2-like protein B2; retinoid receptor-induced gene 1"
                     /codon_start=1
                     /product="endophilin-B2 isoform b"
                     /protein_id="NP_064530.1"
                     /db_xref="CCDS:CCDS6916.1"
                     /db_xref="GeneID:56904"
                     /db_xref="HGNC:HGNC:10834"
                     /db_xref="MIM:609288"
                     /translation="MDFNMKKLASDAGIFFTRAVQFTEEKFGQAEKTELDAHFENLLA
                     RADSTKNWTEKILRQTEVLLQPNPSARVEEFLYEKLDRKVPSRVTNGELLAQYMADAA
                     SELGPTTPYGKTLIKVAEAEKQLGAAERDFIHTASISFLTPLRNFLEGDWKTISKERR
                     LLQNRRLDLDACKARLKKAKAAEAKATTVPDFQETRPRNYILSASASALWNDEVDKAE
                     QELRVAQTEFDRQAEVTRLLLEGISSTHVNHLRCLHEFVKSQTTYYAQCYRHMLDLQK
                     QLGRFPGTFVGTTEPASPPLSSTSPTTAAATMPVVPSVASLAPPGEASLCLEEVAPPA
                     SGTRKARVLYDYEAADSSELALLADELITVYSLPGMDPDWLIGERGNKKGKVPVTYLE
                     LLS"
     exon            241..382
                     /gene="SH3GLB2"
                     /gene_synonym="PP6569; PP9455; RRIG1"
                     /inference="alignment:Splign:2.1.0"
     exon            383..511
                     /gene="SH3GLB2"
                     /gene_synonym="PP6569; PP9455; RRIG1"
                     /inference="alignment:Splign:2.1.0"
     exon            512..645
                     /gene="SH3GLB2"
                     /gene_synonym="PP6569; PP9455; RRIG1"
                     /inference="alignment:Splign:2.1.0"
     exon            646..738
                     /gene="SH3GLB2"
                     /gene_synonym="PP6569; PP9455; RRIG1"
                     /inference="alignment:Splign:2.1.0"
     exon            739..801
                     /gene="SH3GLB2"
                     /gene_synonym="PP6569; PP9455; RRIG1"
                     /inference="alignment:Splign:2.1.0"
     exon            802..825
                     /gene="SH3GLB2"
                     /gene_synonym="PP6569; PP9455; RRIG1"
                     /inference="alignment:Splign:2.1.0"
     exon            826..915
                     /gene="SH3GLB2"
                     /gene_synonym="PP6569; PP9455; RRIG1"
                     /inference="alignment:Splign:2.1.0"
     exon            916..1016
                     /gene="SH3GLB2"
                     /gene_synonym="PP6569; PP9455; RRIG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1017..1257
                     /gene="SH3GLB2"
                     /gene_synonym="PP6569; PP9455; RRIG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1258..3013
                     /gene="SH3GLB2"
                     /gene_synonym="PP6569; PP9455; RRIG1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1990..1995
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SH3GLB2"
                     /gene_synonym="PP6569; PP9455; RRIG1"
                     /note="hexamer: AATAAA"
     polyA_site      2011
                     /gene="SH3GLB2"
                     /gene_synonym="PP6569; PP9455; RRIG1"
                     /note="major polyA site"
     regulatory      2240..2245
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SH3GLB2"
                     /gene_synonym="PP6569; PP9455; RRIG1"
                     /note="hexamer: AAGAAA"
     polyA_site      2257
                     /gene="SH3GLB2"
                     /gene_synonym="PP6569; PP9455; RRIG1"
     regulatory      2961..2966
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SH3GLB2"
                     /gene_synonym="PP6569; PP9455; RRIG1"
                     /note="hexamer: AACAAG"
     regulatory      2984..2989
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SH3GLB2"
                     /gene_synonym="PP6569; PP9455; RRIG1"
                     /note="hexamer: AATAGA"
     polyA_site      2990
                     /gene="SH3GLB2"
                     /gene_synonym="PP6569; PP9455; RRIG1"
     polyA_site      3013
                     /gene="SH3GLB2"
                     /gene_synonym="PP6569; PP9455; RRIG1"
ORIGIN      
        1 ctgcgcgctc gggctggctc ggcggctgcg gcgggcgggc aggcgggcag gccggcaggc
       61 gggtgcgcgg agggctggtg ccccgcagca ggtgggcggg gtgcggttgg cggcggcggc
      121 tgggccgggg gctgccggct gcgctcgggc cgtgcgcggc ggccgtgcgg gcacgccatg
      181 gacttcaaca tgaagaagct ggcgtcggac gcgggcatct tcttcacccg ggcggtgcag
      241 ttcacggagg agaaatttgg ccaggctgag aagactgagc ttgatgccca ctttgaaaac
      301 cttctggccc gggcagacag caccaagaac tggacagaga agatcttgag gcagacagag
      361 gtgctgctgc agcccaaccc cagtgcccga gtggaggagt tcctgtatga gaagctggac
      421 aggaaggtcc cctcaagggt caccaacggg gagctgctgg ctcagtacat ggcagacgcg
      481 gccagtgagc tggggccgac caccccctat gggaagacac tgatcaaggt ggcagaagct
      541 gaaaagcaac tgggagccgc ggagagggat tttatccaca cggcctccat cagcttcctc
      601 acacccttgc gcaacttcct ggagggggac tggaagacca tctcgaagga gaggcggctc
      661 ctccaaaacc ggcgtctgga cttggatgcc tgcaaagcga ggctgaagaa ggccaaggct
      721 gcagaagcca aagccacgac ggtgcctgac tttcaggaga ctagacctcg taattacatt
      781 ctctcggcca gcgcctccgc gctctggaat gatgaagtgg acaaggccga gcaggagctc
      841 cgcgtggccc agacagagtt tgaccggcaa gcagaagtga cccgtctctt gctggaggga
      901 atcagtagca ctcacgtgaa ccacctgcgc tgcctccacg agttcgtcaa gtctcagaca
      961 acctactacg cacagtgcta ccgccacatg ctggacttgc agaagcagct gggcagattt
     1021 cccggcacct tcgtgggcac cacagagccc gcctccccac ccctgagcag cacctcaccc
     1081 accactgctg cggccactat gcctgtggtg ccctctgtgg ccagcctggc ccctccgggg
     1141 gaggcctcgc tctgcctgga agaggtggcc ccccctgcca gtgggacccg caaagctcgg
     1201 gtgctctatg actacgaggc agccgacagc agtgagctgg ccctgctggc tgatgagctc
     1261 atcactgtct acagcctgcc tggcatggac cctgactggc tcattggcga gagaggcaac
     1321 aagaagggca aggtccctgt cacctacttg gaactgctca gctaggcagg tgcccccatc
     1381 ccccccgcat tctggcctag gcaggagagg atgggcgcag ccctgccact taacttgttt
     1441 gttggtgaca cagttgttca gagtggggag aattcacccc attctgtccc tgcccctagt
     1501 cacctagctg tgagggtgcc tgaggctgaa tggctccacc cctcccccag ccctgcttct
     1561 gacctgtggc tctggagccc ctgcccctgc ctgcatcccc gagcacccca ccctccaggc
     1621 tccactaagg agggaggggc tgtctgcagc agctgcactc agcacctagg ccagggtggg
     1681 gccgccgcag atgggctcag gaagccccag gtgcactcag cgagagccct gcctttcagt
     1741 tgccaaaagc tgcatcaggg gaatgcggca aggcacacag ggctctggca gcccctgggg
     1801 actgggcgct gcccctggga ggggagagcc tggccagggc tggtgttggg cccggagcag
     1861 catcttccgg tgctatcctc ccctcccacc cctcacagct caagccaagt ccagcggccg
     1921 cagtcttcac ctctccacac tcacttttta tctggtgttt ttacttctgc ctgcgtttgc
     1981 tctctagcca ataaaccgtc cttgtgtgcg agtcacctgg gctcctgtca gggcctggcc
     2041 ctgagggtgg taaagggaaa ggtagactca ccctctgccc cactcttcca cagagtgagc
     2101 aggggccctg tggctgcccc ggcccccagc tctctagttg ggaagaagtg cagccctccc
     2161 tgtcttctgc ggggcctgtg cctctcagag ggcagtgctg ttcctggaca tgaagccgca
     2221 caaggaacgt tttattccta agaaaaagtt tcctaaattt ggattgtgga gcggactgta
     2281 gaatgcgtct gacagttcag ttcaagcaca agaagtgggt agcggtcagt gctgtctgca
     2341 ggtcgggcgt ttcacccagc atgtgccccc gtgccatggg ttctcatgcc gtcctcagtg
     2401 tccacatttc acagatgaga tcagcaagac agtcagctcc tcaagggacc acagttggca
     2461 agtggccaag cagggactga agcccaggca gcctgattct agaaacccta agtgatgtct
     2521 gtgttttaga tggcccctag caggacctag cattctgact tcagtcctga gaaggccaac
     2581 agccagtagg ccccactgtt ggggcttctg aagaggggat cgggggatga cgggggcacg
     2641 gggctataaa taggaaggca cagagcttca agaaaggctc acccagggat gagctcagcc
     2701 cactgggcca atgaaagcag ggtcaaggac aggaccagcg caggccaagg aagggaatat
     2761 ctgacagcgc ccacccagcc aaaccctcag cccaaggaca ggaatgagga gatgctggtg
     2821 aactagccat ccatcagtac ctgccttccc ccgaggctgc agccccactc ccaggcgcct
     2881 ggccagggga gttttctagg ttctgagagc cacgttgtca tccctgggct ttgaagttaa
     2941 acatcacaca gctgtctata aacaagattt taatatctgt ataaatagac cacagaatta
     3001 ccaaaactgc aaa
//
