LOCUS       NM_170600               3051 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens SH2 domain containing 3C (SH2D3C), transcript variant
            1, mRNA.
ACCESSION   NM_170600
VERSION     NM_170600.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Leung,K.K., Hause,R.J. Jr., Barkinge,J.L., Ciaccio,M.F., Chuu,C.P.
            and Jones,R.B.
  TITLE     Enhanced prediction of Src homology 2 (SH2) domain binding
            potentials using a fluorescence polarization-derived c-Met, c-Kit,
            ErbB, and androgen receptor interactome
  JOURNAL   Mol Cell Proteomics 13 (7), 1705-1723 (2014)
   PUBMED   24728074
REFERENCE   3  (bases 1 to 3051)
  AUTHORS   Wade,T.D., Gordon,S., Medland,S., Bulik,C.M., Heath,A.C.,
            Montgomery,G.W. and Martin,N.G.
  TITLE     Genetic variants associated with disordered eating
  JOURNAL   Int J Eat Disord 46 (6), 594-608 (2013)
   PUBMED   23568457
REFERENCE   4  (bases 1 to 3051)
  AUTHORS   Mace,P.D., Wallez,Y., Dobaczewska,M.K., Lee,J.J., Robinson,H.,
            Pasquale,E.B. and Riedl,S.J.
  TITLE     NSP-Cas protein structures reveal a promiscuous interaction module
            in cell signaling
  JOURNAL   Nat Struct Mol Biol 18 (12), 1381-1387 (2011)
   PUBMED   22081014
  REMARK    GeneRIF: The structure of the NSP3-p130Cas complex reveals that
            this closed conformation is instrumental for interaction of NSP
            proteins with a focal adhesion-targeting domain present in Cas
            proteins.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3051)
  AUTHORS   Near,R.I., Zhang,Y., Makkinje,A., Vanden Borre,P. and Lerner,A.
  TITLE     AND-34/BCAR3 differs from other NSP homologs in induction of
            anti-estrogen resistance, cyclin D1 promoter activation and altered
            breast cancer cell morphology
  JOURNAL   J Cell Physiol 212 (3), 655-665 (2007)
   PUBMED   17427198
  REMARK    GeneRIF: NSP3 overexpression did not induce anti-estrogen
            resistance in breast tumor cell lines.
REFERENCE   6  (bases 1 to 3051)
  AUTHORS   Dail,M., Kalo,M.S., Seddon,J.A., Cote,J.F., Vuori,K. and
            Pasquale,E.B.
  TITLE     SHEP1 function in cell migration is impaired by a single amino acid
            mutation that disrupts association with the scaffolding protein cas
            but not with Ras GTPases
  JOURNAL   J Biol Chem 279 (40), 41892-41902 (2004)
   PUBMED   15272013
REFERENCE   7  (bases 1 to 3051)
  AUTHORS   Sakakibara,A., Hattori,S., Nakamura,S. and Katagiri,T.
  TITLE     A novel hematopoietic adaptor protein, Chat-H, positively regulates
            T cell receptor-mediated interleukin-2 production by Jurkat cells
  JOURNAL   J Biol Chem 278 (8), 6012-6017 (2003)
   PUBMED   12486027
  REMARK    GeneRIF: This protein positively regulates T cell receptor-mediated
            interleukin-2 production by Jurkat cells.
REFERENCE   8  (bases 1 to 3051)
  AUTHORS   Sakakibara,A. and Hattori,S.
  TITLE     Chat, a Cas/HEF1-associated adaptor protein that integrates
            multiple signaling pathways
  JOURNAL   J Biol Chem 275 (9), 6404-6410 (2000)
   PUBMED   10692442
REFERENCE   9  (bases 1 to 3051)
  AUTHORS   Dodelet,V.C., Pazzagli,C., Zisch,A.H., Hauser,C.A. and
            Pasquale,E.B.
  TITLE     A novel signaling intermediate, SHEP1, directly couples Eph
            receptors to R-Ras and Rap1A
  JOURNAL   J Biol Chem 274 (45), 31941-31946 (1999)
   PUBMED   10542222
REFERENCE   10 (bases 1 to 3051)
  AUTHORS   Lu,Y., Brush,J. and Stewart,T.A.
  TITLE     NSP1 defines a novel family of adaptor proteins linking integrin
            and tyrosine kinase receptors to the c-Jun N-terminal
            kinase/stress-activated protein kinase signaling pathway
  JOURNAL   J Biol Chem 274 (15), 10047-10052 (1999)
   PUBMED   10187783
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB161948.1, BC032365.1,
            CU454732.1 and AI799905.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_170600.2.
            
            Summary: This gene encodes an adaptor protein and member of a
            cytoplasmic protein family involved in cell migration. The encoded
            protein contains a putative Src homology 2 (SH2) domain and guanine
            nucleotide exchange factor-like domain which allows this signaling
            protein to form a complex with scaffolding protein Crk-associated
            substrate. Multiple transcript variants encoding different isoforms
            have been found for this gene. [provided by RefSeq, Nov 2011].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK292718.1, BC032365.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000314830.13/ ENSP00000317817.8
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-33                DB161948.1         104-136
            34-2120             BC032365.1         12-2098
            2121-2126           CU454732.1         100-105
            2127-3049           BC032365.1         2105-3027
            3050-3051           AI799905.1         12-13               c
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q34.11"
     gene            1..3051
                     /gene="SH2D3C"
                     /gene_synonym="CHAT; NSP3; PRO34088; SHEP1"
                     /note="SH2 domain containing 3C"
                     /db_xref="GeneID:10044"
                     /db_xref="HGNC:HGNC:16884"
                     /db_xref="MIM:604722"
     exon            1..76
                     /gene="SH2D3C"
                     /gene_synonym="CHAT; NSP3; PRO34088; SHEP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             40..2622
                     /gene="SH2D3C"
                     /gene_synonym="CHAT; NSP3; PRO34088; SHEP1"
                     /note="isoform a is encoded by transcript variant 1; novel
                     SH2-containing protein 3; SH2 domain-containing Eph
                     receptor-binding protein 1; chat-H; cas/HEF1-associated
                     signal transducer"
                     /codon_start=1
                     /product="SH2 domain-containing protein 3C isoform a"
                     /protein_id="NP_733745.1"
                     /db_xref="CCDS:CCDS6877.1"
                     /db_xref="GeneID:10044"
                     /db_xref="HGNC:HGNC:16884"
                     /db_xref="MIM:604722"
                     /translation="MTEGTKKTSKKFKFFKFKGFGSLSNLPRSFTLRRSSASISRQSH
                     LEPDTFEATQDDMVTVPKSPPAYARSSDMYSHMGTMPRPSIKKAQNSQAARQAQEAGP
                     KPNLVPGGVPDPPGLEAAKEVMVKATGPLEDTPAMEPNPSAVEVDPIRKPEVPTGDVE
                     EERPPRDVHSERAAGEPEAGSDYVKFSKEKYILDSSPEKLHKELEEELKLSSTDLRSH
                     AWYHGRIPREVSETLVQRNGDFLIRDSLTSLGDYVLTCRWRNQALHFKINKVVVKAGE
                     SYTHIQYLFEQESFDHVPALVRYHVGSRKAVSEQSGAIIYCPVNRTFPLRYLEASYGL
                     GQGSSKPASPVSPSGPKGSHMKRRSVTMTDGLTADKVTRSDGCPTSTSLPRPRDSIRS
                     CALSMDQIPDLHSPMSPISESPSSPAYSTVTRVHAAPAAPSATALPASPVARRSSEPQ
                     LCPGSAPKTHGESDKGPHTSPSHTLGKASPSPSLSSYSDPDSGHYCQLQPPVRGSREW
                     AATETSSQQARSYGERLKELSENGAPEGDWGKTFTVPIVEVTSSFNPATFQSLLIPRD
                     NRPLEVGLLRKVKELLAEVDARTLARHVTKVDCLVARILGVTKEMQTLMGVRWGMELL
                     TLPHGRQLRLDLLERFHTMSIMLAVDILGCTGSAEERAALLHKTIQLAAELRGTMGNM
                     FSFAAVMGALDMAQISRLEQTWVTLRQRHTEGAILYEKKLKPFLKSLNEGKEGPPLSN
                     TTFPHVLPLITLLECDSAPPEGPEPWGSTEHGVEVVLAHLEAARTVAHHGGLYHTNAE
                     VKLQGFQARPELLEVFSTEFQMRLLWGSQGASSSQARRYEKFDKVLTALSHKLEPAVR
                     SSEL"
     exon            77..554
                     /gene="SH2D3C"
                     /gene_synonym="CHAT; NSP3; PRO34088; SHEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            555..594
                     /gene="SH2D3C"
                     /gene_synonym="CHAT; NSP3; PRO34088; SHEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            595..723
                     /gene="SH2D3C"
                     /gene_synonym="CHAT; NSP3; PRO34088; SHEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            724..1178
                     /gene="SH2D3C"
                     /gene_synonym="CHAT; NSP3; PRO34088; SHEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1179..1303
                     /gene="SH2D3C"
                     /gene_synonym="CHAT; NSP3; PRO34088; SHEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1304..1839
                     /gene="SH2D3C"
                     /gene_synonym="CHAT; NSP3; PRO34088; SHEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1840..1955
                     /gene="SH2D3C"
                     /gene_synonym="CHAT; NSP3; PRO34088; SHEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1956..2127
                     /gene="SH2D3C"
                     /gene_synonym="CHAT; NSP3; PRO34088; SHEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2128..2239
                     /gene="SH2D3C"
                     /gene_synonym="CHAT; NSP3; PRO34088; SHEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2240..2446
                     /gene="SH2D3C"
                     /gene_synonym="CHAT; NSP3; PRO34088; SHEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2447..3051
                     /gene="SH2D3C"
                     /gene_synonym="CHAT; NSP3; PRO34088; SHEP1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3030..3035
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SH2D3C"
                     /gene_synonym="CHAT; NSP3; PRO34088; SHEP1"
                     /note="hexamer: AATAAA"
     polyA_site      3051
                     /gene="SH2D3C"
                     /gene_synonym="CHAT; NSP3; PRO34088; SHEP1"
                     /note="major polyA site"
ORIGIN      
        1 actcgcaagc tggccaaggg cttcacacaa tttgccaaga tgacagaggg gaccaagaag
       61 accagcaaaa agttcaagtt cttcaagttc aagggctttg ggagtctctc caacctccct
      121 cggtccttca ctctgagacg atcctcagct tccatcagta ggcagtccca tttggagcct
      181 gacacctttg aagccacgca ggatgacatg gtgacggtgc ccaagagtcc cccagcctat
      241 gcccgctcca gtgacatgta cagccacatg ggcaccatgc ctcgccccag catcaagaaa
      301 gcacagaact cacaggctgc ccggcaggcc caggaggcgg gtcccaagcc caacttggta
      361 cccggaggtg tacccgaccc cccaggcttg gaggcagcca aagaggtgat ggtgaaggcc
      421 actggccctc tagaggacac cccagcaatg gaacccaacc cttcagcagt ggaggtagac
      481 cccatcagaa agcctgaggt ccccacagga gacgtagaag aggagagacc tcccagggac
      541 gtgcactcag aaagggctgc tggagagcca gaggctggca gcgactatgt gaagttctcc
      601 aaggagaagt acatcctgga ctcatcgcca gagaaactcc acaaggaatt ggaggaggag
      661 ctcaaactca gcagcacgga tctccgcagc catgcctggt accatggccg catcccccga
      721 gaggtctcgg agaccttggt acaacgcaac ggcgacttcc tcatccggga ctcactcacc
      781 agcctgggcg actatgtgct cacgtgccgc tggcgcaacc aggccttgca cttcaagatc
      841 aacaaggtgg tggtgaaggc aggcgagagc tacacacaca tccagtacct gtttgagcag
      901 gagagctttg accacgtgcc cgccctcgtg cgctatcatg tgggcagccg caaggctgtg
      961 tcagagcaga gtggtgccat catctactgc ccggtgaacc gcaccttccc actgcgctac
     1021 ctcgaggcca gctatggcct gggacagggg agtagcaagc ctgctagccc cgtcagcccc
     1081 tcaggcccca agggcagcca catgaagcgg cgcagcgtca ccatgaccga tgggctcact
     1141 gctgacaagg tcacccgcag cgatggctgc cccaccagta cgtcgctgcc ccgccctcgg
     1201 gactccatcc gcagctgtgc cctcagcatg gaccagatcc cagacctgca ctcacccatg
     1261 tcgcccatct ccgagagccc tagctcccct gcctacagca ctgtaacccg tgtccatgcc
     1321 gcccctgcag ccccttctgc cacagcattg cctgcctccc ctgtcgcccg ccgttccagt
     1381 gagccccagc tgtgtcccgg aagtgcccca aagacccatg gggagtcaga caagggcccc
     1441 cacaccagcc cctcccacac ccttggcaag gcctccccgt caccatcact cagcagctac
     1501 agtgacccgg actctggcca ctactgccag ctccagcctc ccgtgcgtgg cagccgagag
     1561 tgggcagcga ctgagacctc cagccagcag gccaggagct atggggagag gctaaaggaa
     1621 ctgtcagaaa atggggcccc tgaaggggac tggggcaaga ccttcacagt ccccatcgtg
     1681 gaagtcactt cttccttcaa cccggccacc ttccagtcac tactgatccc cagggataac
     1741 cggccactgg aggtgggcct tctgcgcaag gtcaaggagc tgctggcaga agtggatgcc
     1801 cggacgctgg cccggcatgt caccaaggtg gactgcctgg ttgctaggat actgggcgtt
     1861 accaaggaga tgcagaccct aatgggagtc cgctggggca tggaactgct caccctcccc
     1921 catggccggc agctacgcct agacctgctg gaaaggttcc acaccatgtc catcatgctg
     1981 gccgtggaca tcctgggctg caccggctct gcggaggagc gggcagcgct gctgcacaag
     2041 accattcagc tggcggccga gctgcggggg actatgggca acatgttcag cttcgcggcg
     2101 gtcatgggtg ccctggacat ggcccagatt tctcggctgg agcagacatg ggtgaccctg
     2161 cggcagcgac acacagaggg tgccatcctg tacgagaaga agctcaagcc ttttctcaag
     2221 agcctcaacg agggcaaaga aggcccgccg ctgagcaaca ccacgtttcc tcatgtgctg
     2281 cccctcatca ccctgctgga gtgtgactcg gccccaccag agggccctga gccctggggc
     2341 agcacggagc acggcgtgga ggtggtgctg gctcacctgg aggccgcccg cacagtggca
     2401 caccacggag gcctgtacca caccaatgct gaagtcaagc tgcaggggtt ccaggcccgg
     2461 ccggagctcc tggaggtgtt cagcacggag ttccagatgc gccttctctg gggcagtcag
     2521 ggtgccagca gcagccaggc ccggcgctat gagaagttcg acaaggtcct cactgccctg
     2581 tcccacaagc tggaacctgc tgtccgctcc agcgagctgt gaccccaggg acatttcccc
     2641 tctgcagctg cggacagcgt caggggcaga ggggcacaca actttcccca gagcacccca
     2701 aggacactgt gatcaacccg agaatgttct gggttcaact caagcatctc ccttgcacct
     2761 ccagggtcct gcgtggactc tgggttccat cccacctgct acatgctcac caggtctcca
     2821 ttgaggaaga acaggaacgc cggttccccc accagctttt gctgctcccc ttcctgctgg
     2881 ggttccctgt tttcgagcca tgggaggcag gctgctcacg cctcctcact ctctgtctgt
     2941 ccctcaccaa caccaaggcc tccatctcac tgtaaataag tctctgttct gtaaatagat
     3001 gtacagaagc catgttattt ctttcatata ataaactttt atgactcttt a
//
