LOCUS       NM_005489               2606 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens SH2 domain containing 3C (SH2D3C), transcript variant
            2, mRNA.
ACCESSION   NM_005489
VERSION     NM_005489.4
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Leung,K.K., Hause,R.J. Jr., Barkinge,J.L., Ciaccio,M.F., Chuu,C.P.
            and Jones,R.B.
  TITLE     Enhanced prediction of Src homology 2 (SH2) domain binding
            potentials using a fluorescence polarization-derived c-Met, c-Kit,
            ErbB, and androgen receptor interactome
  JOURNAL   Mol Cell Proteomics 13 (7), 1705-1723 (2014)
   PUBMED   24728074
REFERENCE   3  (bases 1 to 2606)
  AUTHORS   Wade,T.D., Gordon,S., Medland,S., Bulik,C.M., Heath,A.C.,
            Montgomery,G.W. and Martin,N.G.
  TITLE     Genetic variants associated with disordered eating
  JOURNAL   Int J Eat Disord 46 (6), 594-608 (2013)
   PUBMED   23568457
REFERENCE   4  (bases 1 to 2606)
  AUTHORS   Mace,P.D., Wallez,Y., Dobaczewska,M.K., Lee,J.J., Robinson,H.,
            Pasquale,E.B. and Riedl,S.J.
  TITLE     NSP-Cas protein structures reveal a promiscuous interaction module
            in cell signaling
  JOURNAL   Nat Struct Mol Biol 18 (12), 1381-1387 (2011)
   PUBMED   22081014
  REMARK    GeneRIF: The structure of the NSP3-p130Cas complex reveals that
            this closed conformation is instrumental for interaction of NSP
            proteins with a focal adhesion-targeting domain present in Cas
            proteins.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2606)
  AUTHORS   Near,R.I., Zhang,Y., Makkinje,A., Vanden Borre,P. and Lerner,A.
  TITLE     AND-34/BCAR3 differs from other NSP homologs in induction of
            anti-estrogen resistance, cyclin D1 promoter activation and altered
            breast cancer cell morphology
  JOURNAL   J Cell Physiol 212 (3), 655-665 (2007)
   PUBMED   17427198
  REMARK    GeneRIF: NSP3 overexpression did not induce anti-estrogen
            resistance in breast tumor cell lines.
REFERENCE   6  (bases 1 to 2606)
  AUTHORS   Dail,M., Kalo,M.S., Seddon,J.A., Cote,J.F., Vuori,K. and
            Pasquale,E.B.
  TITLE     SHEP1 function in cell migration is impaired by a single amino acid
            mutation that disrupts association with the scaffolding protein cas
            but not with Ras GTPases
  JOURNAL   J Biol Chem 279 (40), 41892-41902 (2004)
   PUBMED   15272013
REFERENCE   7  (bases 1 to 2606)
  AUTHORS   Sakakibara,A., Hattori,S., Nakamura,S. and Katagiri,T.
  TITLE     A novel hematopoietic adaptor protein, Chat-H, positively regulates
            T cell receptor-mediated interleukin-2 production by Jurkat cells
  JOURNAL   J Biol Chem 278 (8), 6012-6017 (2003)
   PUBMED   12486027
  REMARK    GeneRIF: This protein positively regulates T cell receptor-mediated
            interleukin-2 production by Jurkat cells.
REFERENCE   8  (bases 1 to 2606)
  AUTHORS   Sakakibara,A. and Hattori,S.
  TITLE     Chat, a Cas/HEF1-associated adaptor protein that integrates
            multiple signaling pathways
  JOURNAL   J Biol Chem 275 (9), 6404-6410 (2000)
   PUBMED   10692442
REFERENCE   9  (bases 1 to 2606)
  AUTHORS   Dodelet,V.C., Pazzagli,C., Zisch,A.H., Hauser,C.A. and
            Pasquale,E.B.
  TITLE     A novel signaling intermediate, SHEP1, directly couples Eph
            receptors to R-Ras and Rap1A
  JOURNAL   J Biol Chem 274 (45), 31941-31946 (1999)
   PUBMED   10542222
REFERENCE   10 (bases 1 to 2606)
  AUTHORS   Lu,Y., Brush,J. and Stewart,T.A.
  TITLE     NSP1 defines a novel family of adaptor proteins linking integrin
            and tyrosine kinase receptors to the c-Jun N-terminal
            kinase/stress-activated protein kinase signaling pathway
  JOURNAL   J Biol Chem 274 (15), 10047-10052 (1999)
   PUBMED   10187783
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BI912337.1, BC027962.1 and
            AL162426.20.
            
            On May 31, 2019 this sequence version replaced NM_005489.3.
            
            Summary: This gene encodes an adaptor protein and member of a
            cytoplasmic protein family involved in cell migration. The encoded
            protein contains a putative Src homology 2 (SH2) domain and guanine
            nucleotide exchange factor-like domain which allows this signaling
            protein to form a complex with scaffolding protein Crk-associated
            substrate. Multiple transcript variants encoding different isoforms
            have been found for this gene. [provided by RefSeq, Nov 2011].
            
            Transcript Variant: This variant (2) includes alternate exons in
            the 5' coding region, compared to variant 1. It encodes isoform b,
            which is shorter and has a distinct N-terminus compared to isoform
            a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC027962.1, AK292041.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-54                BI912337.1         145-198
            55-1678             BC027962.1         1-1624
            1679-1679           AL162426.20        155104-155104       c
            1680-2606           BC027962.1         1626-2552
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q34.11"
     gene            1..2606
                     /gene="SH2D3C"
                     /gene_synonym="CHAT; NSP3; PRO34088; SHEP1"
                     /note="SH2 domain containing 3C"
                     /db_xref="GeneID:10044"
                     /db_xref="HGNC:HGNC:16884"
                     /db_xref="MIM:604722"
     exon            1..109
                     /gene="SH2D3C"
                     /gene_synonym="CHAT; NSP3; PRO34088; SHEP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             66..2177
                     /gene="SH2D3C"
                     /gene_synonym="CHAT; NSP3; PRO34088; SHEP1"
                     /note="isoform b is encoded by transcript variant 2; novel
                     SH2-containing protein 3; SH2 domain-containing Eph
                     receptor-binding protein 1; chat-H; cas/HEF1-associated
                     signal transducer"
                     /codon_start=1
                     /product="SH2 domain-containing protein 3C isoform b"
                     /protein_id="NP_005480.2"
                     /db_xref="CCDS:CCDS6878.1"
                     /db_xref="GeneID:10044"
                     /db_xref="HGNC:HGNC:16884"
                     /db_xref="MIM:604722"
                     /translation="MTAVGRRCPALGSRGAAGEPEAGSDYVKFSKEKYILDSSPEKLH
                     KELEEELKLSSTDLRSHAWYHGRIPREVSETLVQRNGDFLIRDSLTSLGDYVLTCRWR
                     NQALHFKINKVVVKAGESYTHIQYLFEQESFDHVPALVRYHVGSRKAVSEQSGAIIYC
                     PVNRTFPLRYLEASYGLGQGSSKPASPVSPSGPKGSHMKRRSVTMTDGLTADKVTRSD
                     GCPTSTSLPRPRDSIRSCALSMDQIPDLHSPMSPISESPSSPAYSTVTRVHAAPAAPS
                     ATALPASPVARRSSEPQLCPGSAPKTHGESDKGPHTSPSHTLGKASPSPSLSSYSDPD
                     SGHYCQLQPPVRGSREWAATETSSQQARSYGERLKELSENGAPEGDWGKTFTVPIVEV
                     TSSFNPATFQSLLIPRDNRPLEVGLLRKVKELLAEVDARTLARHVTKVDCLVARILGV
                     TKEMQTLMGVRWGMELLTLPHGRQLRLDLLERFHTMSIMLAVDILGCTGSAEERAALL
                     HKTIQLAAELRGTMGNMFSFAAVMGALDMAQISRLEQTWVTLRQRHTEGAILYEKKLK
                     PFLKSLNEGKEGPPLSNTTFPHVLPLITLLECDSAPPEGPEPWGSTEHGVEVVLAHLE
                     AARTVAHHGGLYHTNAEVKLQGFQARPELLEVFSTEFQMRLLWGSQGASSSQARRYEK
                     FDKVLTALSHKLEPAVRSSEL"
     exon            110..149
                     /gene="SH2D3C"
                     /gene_synonym="CHAT; NSP3; PRO34088; SHEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            150..278
                     /gene="SH2D3C"
                     /gene_synonym="CHAT; NSP3; PRO34088; SHEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            279..733
                     /gene="SH2D3C"
                     /gene_synonym="CHAT; NSP3; PRO34088; SHEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            734..858
                     /gene="SH2D3C"
                     /gene_synonym="CHAT; NSP3; PRO34088; SHEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            859..1394
                     /gene="SH2D3C"
                     /gene_synonym="CHAT; NSP3; PRO34088; SHEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1395..1510
                     /gene="SH2D3C"
                     /gene_synonym="CHAT; NSP3; PRO34088; SHEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1511..1682
                     /gene="SH2D3C"
                     /gene_synonym="CHAT; NSP3; PRO34088; SHEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1683..1794
                     /gene="SH2D3C"
                     /gene_synonym="CHAT; NSP3; PRO34088; SHEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1795..2001
                     /gene="SH2D3C"
                     /gene_synonym="CHAT; NSP3; PRO34088; SHEP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2002..2606
                     /gene="SH2D3C"
                     /gene_synonym="CHAT; NSP3; PRO34088; SHEP1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2585..2590
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SH2D3C"
                     /gene_synonym="CHAT; NSP3; PRO34088; SHEP1"
                     /note="hexamer: AATAAA"
     polyA_site      2606
                     /gene="SH2D3C"
                     /gene_synonym="CHAT; NSP3; PRO34088; SHEP1"
                     /note="major polyA site"
ORIGIN      
        1 gagaaaggca ggagccgctg ggtttaggag gtccccgggt tgccggcggc gacagcgggg
       61 gaagcatgac tgctgtgggc cgaaggtgcc ccgcgctggg gtcccgaggg gctgctggag
      121 agccagaggc tggcagcgac tatgtgaagt tctccaagga gaagtacatc ctggactcat
      181 cgccagagaa actccacaag gaattggagg aggagctcaa actcagcagc acggatctcc
      241 gcagccatgc ctggtaccat ggccgcatcc cccgagaggt ctcggagacc ttggtacaac
      301 gcaacggcga cttcctcatc cgggactcac tcaccagcct gggcgactat gtgctcacgt
      361 gccgctggcg caaccaggcc ttgcacttca agatcaacaa ggtggtggtg aaggcaggcg
      421 agagctacac acacatccag tacctgtttg agcaggagag ctttgaccac gtgcccgccc
      481 tcgtgcgcta tcatgtgggc agccgcaagg ctgtgtcaga gcagagtggt gccatcatct
      541 actgcccggt gaaccgcacc ttcccactgc gctacctcga ggccagctat ggcctgggac
      601 aggggagtag caagcctgct agccccgtca gcccctcagg ccccaagggc agccacatga
      661 agcggcgcag cgtcaccatg accgatgggc tcactgctga caaggtcacc cgcagcgatg
      721 gctgccccac cagtacgtcg ctgccccgcc ctcgggactc catccgcagc tgtgccctca
      781 gcatggacca gatcccagac ctgcactcac ccatgtcgcc catctccgag agccctagct
      841 cccctgccta cagcactgta acccgtgtcc atgccgcccc tgcagcccct tctgccacag
      901 cattgcctgc ctcccctgtc gcccgccgtt ccagtgagcc ccagctgtgt cccggaagtg
      961 ccccaaagac ccatggggag tcagacaagg gcccccacac cagcccctcc cacacccttg
     1021 gcaaggcctc cccgtcacca tcactcagca gctacagtga cccggactct ggccactact
     1081 gccagctcca gcctcccgtg cgtggcagcc gagagtgggc agcgactgag acctccagcc
     1141 agcaggccag gagctatggg gagaggctaa aggaactgtc agaaaatggg gcccctgaag
     1201 gggactgggg caagaccttc acagtcccca tcgtggaagt cacttcttcc ttcaacccgg
     1261 ccaccttcca gtcactactg atccccaggg ataaccggcc actggaggtg ggccttctgc
     1321 gcaaggtcaa ggagctgctg gcagaagtgg atgcccggac gctggcccgg catgtcacca
     1381 aggtggactg cctggttgct aggatactgg gcgttaccaa ggagatgcag accctaatgg
     1441 gagtccgctg gggcatggaa ctgctcaccc tcccccatgg ccggcagcta cgcctagacc
     1501 tgctggaaag gttccacacc atgtccatca tgctggccgt ggacatcctg ggctgcaccg
     1561 gctctgcgga ggagcgggca gcgctgctgc acaagaccat tcagctggcg gccgagctgc
     1621 gggggactat gggcaacatg ttcagcttcg cggcggtcat gggtgccctg gacatggccc
     1681 agatttctcg gctggagcag acatgggtga ccctgcggca gcgacacaca gagggtgcca
     1741 tcctgtacga gaagaagctc aagccttttc tcaagagcct caacgagggc aaagaaggcc
     1801 cgccgctgag caacaccacg tttcctcatg tgctgcccct catcaccctg ctggagtgtg
     1861 actcggcccc accagagggc cctgagccct ggggcagcac ggagcacggc gtggaggtgg
     1921 tgctggctca cctggaggcc gcccgcacag tggcacacca cggaggcctg taccacacca
     1981 atgctgaagt caagctgcag gggttccagg cccggccgga gctcctggag gtgttcagca
     2041 cggagttcca gatgcgcctt ctctggggca gtcagggtgc cagcagcagc caggcccggc
     2101 gctatgagaa gttcgacaag gtcctcactg ccctgtccca caagctggaa cctgctgtcc
     2161 gctccagcga gctgtgaccc cagggacatt tcccctctgc agctgcggac agcgtcaggg
     2221 gcagaggggc acacaacttt ccccagagca ccccaaggac actgtgatca acccgagaat
     2281 gttctgggtt caactcaagc atctcccttg cacctccagg gtcctgcgtg gactctgggt
     2341 tccatcccac ctgctacatg ctcaccaggt ctccattgag gaagaacagg aacgccggtt
     2401 cccccaccag cttttgctgc tccccttcct gctggggttc cctgttttcg agccatggga
     2461 ggcaggctgc tcacgcctcc tcactctctg tctgtccctc accaacacca aggcctccat
     2521 ctcactgtaa ataagtctct gttctgtaaa tagatgtaca gaagccatgt tatttctttc
     2581 atataataaa cttttatgac tcttta
//
