LOCUS       NM_005490               2281 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens SH2 domain containing 3A (SH2D3A), transcript variant
            1, mRNA.
ACCESSION   NM_005490
VERSION     NM_005490.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Abaeva,I.S., Arhab,Y., Miscicka,A., Hellen,C.U.T. and Pestova,T.V.
  TITLE     In vitro reconstitution of SARS-CoV-2 Nsp1-induced mRNA cleavage
            reveals the key roles of the N-terminal domain of Nsp1 and the RRM
            domain of eIF3g
  JOURNAL   Genes Dev 37 (17-18), 844-860 (2023)
   PUBMED   37821106
  REMARK    GeneRIF: In vitro reconstitution of SARS-CoV-2 Nsp1-induced mRNA
            cleavage reveals the key roles of the N-terminal domain of Nsp1 and
            the RRM domain of eIF3g.
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Dolliver,S.M., Kleer,M., Bui-Marinos,M.P., Ying,S., Corcoran,J.A.
            and Khaperskyy,D.A.
  TITLE     Nsp1 proteins of human coronaviruses HCoV-OC43 and SARS-CoV2
            inhibit stress granule formation
  JOURNAL   PLoS Pathog 18 (12), e1011041 (2022)
   PUBMED   36534661
  REMARK    GeneRIF: Nsp1 proteins of human coronaviruses HCoV-OC43 and
            SARS-CoV2 inhibit stress granule formation.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2281)
  AUTHORS   Kennedy,S.A., Jarboui,M.A., Srihari,S., Raso,C., Bryan,K.,
            Dernayka,L., Charitou,T., Bernal-Llinares,M., Herrera-Montavez,C.,
            Krstic,A., Matallanas,D., Kotlyar,M., Jurisica,I., Curak,J.,
            Wong,V., Stagljar,I., LeBihan,T., Imrie,L., Pillai,P., Lynn,M.A.,
            Fasterius,E., Al-Khalili Szigyarto,C., Breen,J., Kiel,C.,
            Serrano,L., Rauch,N., Rukhlenko,O., Kholodenko,B.N.,
            Iglesias-Martinez,L.F., Ryan,C.J., Pilkington,R., Cammareri,P.,
            Sansom,O., Shave,S., Auer,M., Horn,N., Klose,F., Ueffing,M.,
            Boldt,K., Lynn,D.J. and Kolch,W.
  TITLE     Extensive rewiring of the EGFR network in colorectal cancer cells
            expressing transforming levels of KRASG13D
  JOURNAL   Nat Commun 11 (1), 499 (2020)
   PUBMED   31980649
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2281)
  AUTHORS   Petschnigg,J., Groisman,B., Kotlyar,M., Taipale,M., Zheng,Y.,
            Kurat,C.F., Sayad,A., Sierra,J.R., Mattiazzi Usaj,M., Snider,J.,
            Nachman,A., Krykbaeva,I., Tsao,M.S., Moffat,J., Pawson,T.,
            Lindquist,S., Jurisica,I. and Stagljar,I.
  TITLE     The mammalian-membrane two-hybrid assay (MaMTH) for probing
            membrane-protein interactions in human cells
  JOURNAL   Nat Methods 11 (5), 585-592 (2014)
   PUBMED   24658140
REFERENCE   5  (bases 1 to 2281)
  AUTHORS   Near,R.I., Zhang,Y., Makkinje,A., Vanden Borre,P. and Lerner,A.
  TITLE     AND-34/BCAR3 differs from other NSP homologs in induction of
            anti-estrogen resistance, cyclin D1 promoter activation and altered
            breast cancer cell morphology
  JOURNAL   J Cell Physiol 212 (3), 655-665 (2007)
   PUBMED   17427198
  REMARK    GeneRIF: NSP1 overexpression did not induce anti-estrogen
            resistance in breast tumor cell lines.
REFERENCE   6  (bases 1 to 2281)
  AUTHORS   Vervoort,V.S., Roselli,S., Oshima,R.G. and Pasquale,E.B.
  TITLE     Splice variants and expression patterns of SHEP1, BCAR3 and NSP1, a
            gene family involved in integrin and receptor tyrosine kinase
            signaling
  JOURNAL   Gene 391 (1-2), 161-170 (2007)
   PUBMED   17270363
  REMARK    GeneRIF: NSP1 and BCAR3 are more highly expressed than SH2D3C
            (SHEP1) in breast cancer cells, and the expression patterns suggest
            differential roles for the three genes during breast cancer
            progression.
REFERENCE   7  (bases 1 to 2281)
  AUTHORS   Clark,H.F., Gurney,A.L., Abaya,E., Baker,K., Baldwin,D., Brush,J.,
            Chen,J., Chow,B., Chui,C., Crowley,C., Currell,B., Deuel,B.,
            Dowd,P., Eaton,D., Foster,J., Grimaldi,C., Gu,Q., Hass,P.E.,
            Heldens,S., Huang,A., Kim,H.S., Klimowski,L., Jin,Y., Johnson,S.,
            Lee,J., Lewis,L., Liao,D., Mark,M., Robbie,E., Sanchez,C.,
            Schoenfeld,J., Seshagiri,S., Simmons,L., Singh,J., Smith,V.,
            Stinson,J., Vagts,A., Vandlen,R., Watanabe,C., Wieand,D., Woods,K.,
            Xie,M.H., Yansura,D., Yi,S., Yu,G., Yuan,J., Zhang,M., Zhang,Z.,
            Goddard,A., Wood,W.I., Godowski,P. and Gray,A.
  TITLE     The secreted protein discovery initiative (SPDI), a large-scale
            effort to identify novel human secreted and transmembrane proteins:
            a bioinformatics assessment
  JOURNAL   Genome Res 13 (10), 2265-2270 (2003)
   PUBMED   12975309
  REMARK    Erratum:[Genome Res. 2003 Dec;13(12):2759]
REFERENCE   8  (bases 1 to 2281)
  AUTHORS   Lu,Y., Brush,J. and Stewart,T.A.
  TITLE     NSP1 defines a novel family of adaptor proteins linking integrin
            and tyrosine kinase receptors to the c-Jun N-terminal
            kinase/stress-activated protein kinase signaling pathway
  JOURNAL   J Biol Chem 274 (15), 10047-10052 (1999)
   PUBMED   10187783
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC006281.2.
            
            On Nov 22, 2018 this sequence version replaced NM_005490.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC006281.2, AF124249.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000245908.11/ ENSP00000245908.5
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2281              BC006281.2         15-2295
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19p13.3"
     gene            1..2281
                     /gene="SH2D3A"
                     /gene_synonym="NSP1"
                     /note="SH2 domain containing 3A"
                     /db_xref="GeneID:10045"
                     /db_xref="HGNC:HGNC:16885"
                     /db_xref="MIM:604721"
     exon            1..49
                     /gene="SH2D3A"
                     /gene_synonym="NSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            50..186
                     /gene="SH2D3A"
                     /gene_synonym="NSP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             118..1848
                     /gene="SH2D3A"
                     /gene_synonym="NSP1"
                     /note="isoform 1 is encoded by transcript variant 1; novel
                     SH2-containing protein 1; SH2 domain-containing protein
                     3A"
                     /codon_start=1
                     /product="SH2 domain-containing protein 3A isoform 1"
                     /protein_id="NP_005481.2"
                     /db_xref="CCDS:CCDS12173.1"
                     /db_xref="GeneID:10045"
                     /db_xref="HGNC:HGNC:16885"
                     /db_xref="MIM:604721"
                     /translation="MQVPQDGEDLAGQPWYHGLLSRQKAEALLQQNGDFLVRASGSRG
                     GNPVISCRWRGSALHFEVFRVALRPRPGRPTALFQLEDEQFPSIPALVHSYMTGRRPL
                     SQATGAVVSRPVTWQGPLRRSFSEDTLMDGPARIEPLRARKWSNSQPADLAHMGRSRE
                     DPAGMEASTMPISALPRTSSDPVLLKAPAPLGTVADSLRASDGQLQAKAPTKPPRTPS
                     FELPDASERPPTYCELVPRVPSVQGTSPSQSCPEPEAPWWEAEEDEEEENRCFTRPQA
                     EISFCPHDAPSCLLGPQNRPLEPQVLHTLRGLFLEHHPGSTALHLLLVDCQATGLLGV
                     TRDQRGNMGVSSGLELLTLPHGHHLRLELLERHQTLALAGALAVLGCSGPLEERAAAL
                     RGLVELALALRPGAAGDLPGLAAVMGALLMPQVSRLEHTWRQLRRSHTEAALAFEQEL
                     KPLMRALDEGAGPCDPGEVALPHVAPMVRLLEGEEVAGPLDESCERLLRTLHGARHMV
                     RDAPKFRKVAAQRLRGFRPNPELREALTTGFVRRLLWGSRGAGAPRAERFEKFQRVLG
                     VLSQRLEPDR"
     exon            187..536
                     /gene="SH2D3A"
                     /gene_synonym="NSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            537..613
                     /gene="SH2D3A"
                     /gene_synonym="NSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            614..1098
                     /gene="SH2D3A"
                     /gene_synonym="NSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1099..1214
                     /gene="SH2D3A"
                     /gene_synonym="NSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1215..1389
                     /gene="SH2D3A"
                     /gene_synonym="NSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1390..1501
                     /gene="SH2D3A"
                     /gene_synonym="NSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1502..1687
                     /gene="SH2D3A"
                     /gene_synonym="NSP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1688..2281
                     /gene="SH2D3A"
                     /gene_synonym="NSP1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2255..2260
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SH2D3A"
                     /gene_synonym="NSP1"
                     /note="hexamer: AATAAA"
     polyA_site      2281
                     /gene="SH2D3A"
                     /gene_synonym="NSP1"
                     /note="major polyA site"
ORIGIN      
        1 agcttccgcc tggacccagc ctcgtgggag ccccgcgggt cctgcccaga tgtggaagac
       61 tgaggcctgt tgaaagtgca gagctcagcc ctggcaccct ctgttcccaa gagctccatg
      121 caggtgccac aggatggaga agaccttgct ggccaacctt ggtaccacgg cctcctgtcc
      181 cgccagaagg ctgaagctct tcttcagcaa aatggcgact tcctggttcg cgcctctggg
      241 tcccgtgggg gcaaccccgt gatctcctgc cgctggcggg gctcagccct ccattttgag
      301 gtgttccgtg tggccctgcg tccccggcca ggccgaccca cagccctctt tcaactggag
      361 gatgagcaat tccccagcat accggctctg gttcacagtt atatgacagg caggcgccca
      421 ctgtcccagg ccacaggggc tgtggtctcc aggcctgtga cttggcaggg gcctctgcga
      481 cgcagcttta gcgaggacac cctgatggat ggcccagctc ggatagagcc tctcagggca
      541 aggaagtgga gcaacagtca gcctgcagat ttggcacata tggggcggtc aagagaagac
      601 cccgctggga tggaagcctc caccatgccc atatctgcct tgccccgaac gagcagtgac
      661 ccggtgttgc tgaaggcccc tgctcccctg ggaactgttg ccgacagtct cagggcctcc
      721 gatgggcagc ttcaagccaa ggcaccaacg aagccccccc ggacaccctc cttcgaactg
      781 cctgatgcct ctgaacgtcc cccgacgtac tgcgagctgg tgccccgagt gcccagtgtc
      841 cagggaacat ccccgagcca aagctgccca gagccagagg ccccatggtg ggaggccgag
      901 gaggatgagg aggaagagaa tagatgtttt acaagaccac aggctgagat ctctttctgc
      961 ccccatgatg ccccctcctg cctgctgggc ccccagaatc ggcccctgga accccaagtc
     1021 ctgcataccc tccgtggcct gttcctggag caccatcctg ggagcaccgc ccttcacctg
     1081 ctattggtag actgccaggc cacaggcctc ctgggagtga ccagagatca gcggggcaac
     1141 atgggagtct catctggcct ggagctgctc actcttcccc atggacacca cttgaggttg
     1201 gaactgctgg agaggcatca gacactggcg ctggccgggg cgctggcggt gctgggctgc
     1261 tcggggccgc tggaggagcg cgcagccgca ctgaggggac tggtagagct ggcgctggcg
     1321 ctgcggccag gggcggcggg ggacctgccc gggctggctg cagtcatggg cgccctgctc
     1381 atgccccagg tgtcccggtt ggagcacacg tggcgccagc tccgaaggag ccacacggag
     1441 gctgcgctgg cctttgagca ggagctgaag ccgctgatgc gggctctgga tgagggcgct
     1501 ggaccctgcg accccggcga ggtggcgctg ccgcacgtgg cacccatggt tcgcctactg
     1561 gagggcgagg aagtcgcggg gccgctggac gagagctgtg agcggctgtt gcgcaccctg
     1621 cacggggcgc gtcacatggt ccgggacgca cccaaattcc gcaaggtggc agcccagcgc
     1681 ctgcgaggat tccggcctaa cccggagctg agggaggccc tgaccaccgg cttcgtgcgg
     1741 aggctgctct ggggtagccg gggcgcggga gctccgcgcg ctgaacgctt tgagaagttc
     1801 cagcgcgtcc tcggcgtcct gtcgcagcgc ctggagcctg accgctgaga gcgcagacac
     1861 ccttcttcac acccgggacc cccaggtttt tgcgaacccc agaagagacc aaaggagtcg
     1921 tcccaggctc ctcgcgcctc aggtggaatc ctgccctgtg cctcacagaa gaggtgggga
     1981 ccgcagtcag ggtcacctgg accatggtga acatgtgacc tgcagatctg gcatcagagg
     2041 ccagagttca aatgtgactc cacctcttaa aagccgtgat ttctagcagt tgacttcacc
     2101 tctgtgtcgg cctttaacaa aatcatagcc atacagcagc tcaggcctgt aatctcagca
     2161 ctttgggagg ccgaggcgga aggaaggctt gaggccagga gttcaagacc agccagggca
     2221 acatggtgag acctcatctc tacaaaaact gaaaaataaa aaacttttaa aaaatgtaaa
     2281 a
//
