LOCUS       NM_003975               1644 bp    mRNA    linear   PRI 09-JUN-2024
DEFINITION  Homo sapiens SH2 domain containing 2A (SH2D2A), transcript variant
            2, mRNA.
ACCESSION   NM_003975
VERSION     NM_003975.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Guo,R.Y., Song,S., Wang,J.Q., Guo,J.Y., Liu,J., Jia,Z., Yuan,C.C.
            and Li,B.
  TITLE     Downregulation of lncRNA XIST may promote Th17 differentiation
            through KDM6A-TSAd pathway in neuromyelitis optica spectrum
            disorders
  JOURNAL   Mult Scler Relat Disord 76, 104801 (2023)
   PUBMED   37315471
  REMARK    GeneRIF: Downregulation of lncRNA XIST may promote Th17
            differentiation through KDM6A-TSAd pathway in neuromyelitis optica
            spectrum disorders.
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Yi,F.S., Zhang,X., Zhai,K., Huang,Z.Y., Wu,X.Z., Wu,M.T., Shi,X.Y.,
            Pei,X.B., Dong,S.F., Wang,W., Yang,Y., Du,J., Luo,Z.T. and Shi,H.Z.
  TITLE     TSAd Plays a Major Role in Myo9b-Mediated Suppression of Malignant
            Pleural Effusion by Regulating TH1/TH17 Cell Response
  JOURNAL   J Immunol 205 (10), 2926-2935 (2020)
   PUBMED   33046503
  REMARK    GeneRIF: TSAd Plays a Major Role in Myo9b-Mediated Suppression of
            Malignant Pleural Effusion by Regulating TH1/TH17 Cell Response.
REFERENCE   3  (bases 1 to 1644)
  AUTHORS   Andersen,T.C.B., Kristiansen,P.E., Huszenicza,Z., Johansson,M.U.,
            Gopalakrishnan,R.P., Kjelstrup,H., Boyken,S., Sundvold-Gjerstad,V.,
            Granum,S., Sorli,M., Backe,P.H., Fulton,D.B., Karlsson,B.G.,
            Andreotti,A.H. and Spurkland,A.
  TITLE     The SH3 domains of the protein kinases ITK and LCK compete for
            adjacent sites on T cell-specific adapter protein
  JOURNAL   J Biol Chem 294 (42), 15480-15494 (2019)
   PUBMED   31484725
  REMARK    GeneRIF: The ITK SH3 and LCK SH3 domains can both competefor and
            simultaneously bind to adjacent binding sites on TSAD encompassing
            aa 242-268.
REFERENCE   4  (bases 1 to 1644)
  AUTHORS   Hem,C.D., Ekornhol,M., Granum,S., Sundvold-Gjerstad,V. and
            Spurkland,A.
  TITLE     CD6 and Linker of Activated T Cells are Potential Interaction
            Partners for T Cell-Specific Adaptor Protein
  JOURNAL   Scand J Immunol 85 (2), 104-112 (2017)
   PUBMED   27896837
  REMARK    GeneRIF: Data indicate that the T cell-specific adaptor protein
            (TSAd) SH2 domain interacts with CD6 antigen and linker for
            activation of T cells protein (LAT) phosphotyrosine (pTyr)
            peptides.
REFERENCE   5  (bases 1 to 1644)
  AUTHORS   Dai,K.Z., Harbo,H.F., Celius,E.G., Oturai,A., Sorensen,P.S.,
            Ryder,L.P., Datta,P., Svejgaard,A., Hillert,J., Fredrikson,S.,
            Sandberg-Wollheim,M., Laaksonen,M., Myhr,K.M., Nyland,H.,
            Vartdal,F. and Spurkland,A.
  TITLE     The T cell regulator gene SH2D2A contributes to the genetic
            susceptibility of multiple sclerosis
  JOURNAL   Genes Immun 2 (5), 263-268 (2001)
   PUBMED   11528519
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   6  (bases 1 to 1644)
  AUTHORS   Wu,L.W., Mayo,L.D., Dunbar,J.D., Kessler,K.M., Ozes,O.N.,
            Warren,R.S. and Donner,D.B.
  TITLE     VRAP is an adaptor protein that binds KDR, a receptor for vascular
            endothelial cell growth factor
  JOURNAL   J Biol Chem 275 (9), 6059-6062 (2000)
   PUBMED   10692392
REFERENCE   7  (bases 1 to 1644)
  AUTHORS   Dai,K.Z., Vergnaud,G., Ando,A., Inoko,H. and Spurkland,A.
  TITLE     The SH2D2A gene encoding the T-cell-specific adapter protein (TSAd)
            is localized centromeric to the CD1 gene cluster on human
            Chromosome 1
  JOURNAL   Immunogenetics 51 (3), 179-185 (2000)
   PUBMED   10752626
REFERENCE   8  (bases 1 to 1644)
  AUTHORS   Rajagopal,K., Sommers,C.L., Decker,D.C., Mitchell,E.O.,
            Korthauer,U., Sperling,A.I., Kozak,C.A., Love,P.E. and
            Bluestone,J.A.
  TITLE     RIBP, a novel Rlk/Txk- and itk-binding adaptor protein that
            regulates T cell activation
  JOURNAL   J Exp Med 190 (11), 1657-1668 (1999)
   PUBMED   10587356
REFERENCE   9  (bases 1 to 1644)
  AUTHORS   Choi,Y.B., Kim,C.K. and Yun,Y.
  TITLE     Lad, an adapter protein interacting with the SH2 domain of p56lck,
            is required for T cell activation
  JOURNAL   J Immunol 163 (10), 5242-5249 (1999)
   PUBMED   10553045
REFERENCE   10 (bases 1 to 1644)
  AUTHORS   Spurkland,A., Brinchmann,J.E., Markussen,G., Pedeutour,F.,
            Munthe,E., Lea,T., Vartdal,F. and Aasheim,H.C.
  TITLE     Molecular cloning of a T cell-specific adapter protein (TSAd)
            containing an Src homology (SH) 2 domain and putative SH3 and
            phosphotyrosine binding sites
  JOURNAL   J Biol Chem 273 (8), 4539-4546 (1998)
   PUBMED   9468509
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB128222.1, BC012107.1 and
            AL158169.17.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_003975.3.
            
            Summary: This gene encodes an adaptor protein thought to function
            in T-cell signal transduction. A related protein in mouse is
            responsible for the activation of lymphocyte-specific
            protein-tyrosine kinase and functions in downstream signaling.
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Mar 2010].
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site in the coding region compared to variant 1. The encoded
            isoform (2) is shorter than isoform 1. Variants 2 and 5 encode the
            same isoform (2).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC012107.1, SRR1163658.449433.1
                                           [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000368199.8/ ENSP00000357182.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-38                DB128222.1         1-38
            39-294              BC012107.1         1-256
            295-295             AL158169.17        5218-5218           c
            296-1644            BC012107.1         258-1606
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q23.1"
     gene            1..1644
                     /gene="SH2D2A"
                     /gene_synonym="F2771; SCAP; TSAD; VRAP"
                     /note="SH2 domain containing 2A"
                     /db_xref="GeneID:9047"
                     /db_xref="HGNC:HGNC:10821"
                     /db_xref="MIM:604514"
     exon            1..174
                     /gene="SH2D2A"
                     /gene_synonym="F2771; SCAP; TSAD; VRAP"
                     /inference="alignment:Splign:2.1.0"
     CDS             141..1310
                     /gene="SH2D2A"
                     /gene_synonym="F2771; SCAP; TSAD; VRAP"
                     /note="isoform 2 is encoded by transcript variant 2; T
                     lymphocyte specific adaptor protein; SH2 domain protein
                     2A; VEGF receptor-associated protein; SH2
                     domain-containing protein 2A; T cell specific adapter
                     protein TSAd"
                     /codon_start=1
                     /product="SH2 domain-containing protein 2A isoform 2"
                     /protein_id="NP_003966.2"
                     /db_xref="CCDS:CCDS1159.1"
                     /db_xref="GeneID:9047"
                     /db_xref="HGNC:HGNC:10821"
                     /db_xref="MIM:604514"
                     /translation="MEFPLAQICPQGSHEAPIPTFSTFQITDMTRRSCQNLGYTAASP
                     QAPEAASNTGNAERAEEVPGEGSLFLQAETRAWFQKTQAHWLLQHGAAPAWFHGFITR
                     REAERLLEPKPQGCYLVRFSESAVTFVLTYRSRTCCRHFLLAQLRDGRHVVLGEDSAH
                     ARLQDLLLHYTAHPLSPYGETLTEPLARQTPEPAGLSLRTEESNFGSKSQDPNPQYSP
                     IIKQGQAPVPMQKEGAGEKEPSQLLRPKPPIPAKPQLPPEVYTIPVPRHRPAPRPKPS
                     NPIYNEPDEPIAFYAMGRGSPGEAPSNIYVEVEDEGLPATLGHPVLRKSWSRPVPGGQ
                     NTGGSQLHSENSVIGQGPPLPHQPPPAWRHTLPHNLSRQVLQDRGQAWLPLGPPQ"
     exon            175..263
                     /gene="SH2D2A"
                     /gene_synonym="F2771; SCAP; TSAD; VRAP"
                     /inference="alignment:Splign:2.1.0"
     exon            264..448
                     /gene="SH2D2A"
                     /gene_synonym="F2771; SCAP; TSAD; VRAP"
                     /inference="alignment:Splign:2.1.0"
     exon            449..538
                     /gene="SH2D2A"
                     /gene_synonym="F2771; SCAP; TSAD; VRAP"
                     /inference="alignment:Splign:2.1.0"
     exon            539..707
                     /gene="SH2D2A"
                     /gene_synonym="F2771; SCAP; TSAD; VRAP"
                     /inference="alignment:Splign:2.1.0"
     exon            708..854
                     /gene="SH2D2A"
                     /gene_synonym="F2771; SCAP; TSAD; VRAP"
                     /inference="alignment:Splign:2.1.0"
     exon            855..1142
                     /gene="SH2D2A"
                     /gene_synonym="F2771; SCAP; TSAD; VRAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1143..1313
                     /gene="SH2D2A"
                     /gene_synonym="F2771; SCAP; TSAD; VRAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1314..1644
                     /gene="SH2D2A"
                     /gene_synonym="F2771; SCAP; TSAD; VRAP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1610..1615
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SH2D2A"
                     /gene_synonym="F2771; SCAP; TSAD; VRAP"
                     /note="hexamer: AATAAA"
     polyA_site      1644
                     /gene="SH2D2A"
                     /gene_synonym="F2771; SCAP; TSAD; VRAP"
                     /note="major polyA site"
ORIGIN      
        1 agaactaagg ctgtgggtaa ggcgacattt cctgcccccg gggccagggt gagaggagag
       61 atgatgagtt gctgagtgtg cacacctttc cggaacacat acacacaccc tgctctggga
      121 tcccttgtga ggctgccctc atggagttcc ccctggccca gatatgtccc caagggagtc
      181 acgaagcccc catcccaacc ttcagcacct tccagatcac agacatgacc cgcaggagct
      241 gccagaacct gggctacact gcggcatctc cccaggcccc ggaggctgcc tccaacacag
      301 ggaatgctga gagggcagag gaggtgcctg gagaaggaag cctgttcctg caggccgaga
      361 cccgggcttg gttccagaag acccaggccc actggctcct gcagcacggg gcagcccctg
      421 cctggttcca tggcttcatc acccggaggg aggcagagag gctgctggag cccaagcctc
      481 aggggtgcta cttggtgcgg ttcagcgaga gcgcggtgac cttcgtgctg acttacagga
      541 gccggacttg ctgccgccac ttcctgctgg cccagctcag ggacgggcgc cacgtggtgc
      601 tgggcgagga cagcgcccac gcgcggctgc aggacctgct gctgcactac accgcgcacc
      661 cgctcagccc ctacggggag acgctcaccg agcccctcgc ccgacagact cctgagcctg
      721 caggactttc cctgaggacc gaagaatcaa actttggaag caaaagccag gacccaaacc
      781 cccagtacag cccaatcatc aaacaggggc aagccccagt cccgatgcag aaagaggggg
      841 ccggggagaa ggagccctcc cagctgctca ggcccaagcc tcccatcccc gccaaacctc
      901 agctgccccc agaagtctac acaatccctg ttccacgaca ccgcccggcc ccacgcccca
      961 agccctccaa tcctatctac aatgagcctg atgaacccat agctttctat gccatgggcc
     1021 ggggcagccc tggggaagcc cccagcaaca tctatgtgga agtggaagat gagggcctac
     1081 ccgccaccct tgggcaccct gtcctacgga agagctggtc caggcctgtc ccaggaggcc
     1141 agaatacagg tggctcccag ctgcattctg agaactctgt gattgggcaa ggccctcccc
     1201 tgccccacca gcccccaccc gcctggagac acaccctccc ccacaatctt tctagacagg
     1261 tgcttcagga cagaggacag gcatggcttc cccttgggcc tcctcagtag gcggtctggc
     1321 ctgaccccca acaaagaagc ctggaggtca gagaagcaaa tgcggagcct gctccctcct
     1381 aagaagatcc caagaatcca atggctcagt ccttggtgat ctaagacagc aaagaagtgt
     1441 gcaaggaggg ccctgttagc tcccactgtc ctggtttctc ctcctggagt ctaatttcct
     1501 tggccctctg agccttttga gtctgggccc tggtccaatg ctgctgttgt ctgaggaatg
     1561 gtttggtgag aacagatgtt agaacttgtt tgttgattct tgtctggcta ataaatcatc
     1621 accaactgcc ttctcctaca ggga
//
