LOCUS       NM_032291              10737 bp    mRNA    linear   PRI 03-OCT-2024
DEFINITION  Homo sapiens SH3GL interacting endocytic adaptor 1 (SGIP1),
            transcript variant 1, mRNA.
ACCESSION   NM_032291
VERSION     NM_032291.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10737)
  AUTHORS   Xie,G.F., Xu,Y.X., Xu,F., Sun,L.Y., Ye,Z.L., Ma,J.J., Wang,H.Y. and
            Shao,J.Y.
  TITLE     Plasma SGIP1 methylation in diagnosis and prognosis prediction in
            hepatocellular carcinoma
  JOURNAL   Neoplasma 68 (1), 62-70 (2021)
   PUBMED   33118832
  REMARK    GeneRIF: Plasma SGIP1 methylation in diagnosis and prognosis
            prediction in hepatocellular carcinoma.
REFERENCE   2  (bases 1 to 10737)
  AUTHORS   Zhang,Y., Feng,Y., Xin,Y. and Liu,X.
  TITLE     SGIP1 dimerizes via intermolecular disulfide bond in muHD domain
            during cellular endocytosis
  JOURNAL   Biochem Biophys Res Commun 505 (1), 99-105 (2018)
   PUBMED   30236986
  REMARK    GeneRIF: Data indicate that cysteine C632 residue is important for
            the function of SH3 domain GRB2 like endophilin interacting protein
            1 (SGIP1) during cellular endocytosis.
REFERENCE   3  (bases 1 to 10737)
  AUTHORS   Petko,J., Tranchina,T., Patel,G., Levenson,R. and Justice-Bitner,S.
  TITLE     Identifying novel members of the Wntless interactome through
            genetic and candidate gene approaches
  JOURNAL   Brain Res Bull 138, 96-105 (2018)
   PUBMED   28734904
REFERENCE   4  (bases 1 to 10737)
  AUTHORS   Chwedorowicz,R., Raszewski,G., Kapka-Skrzypczak,L., Sawicki,K. and
            Studzinski,T.
  TITLE     Event-related potentials (ERP) and SGIP1 gene polymorphisms in
            alcoholics: relation to family history of alcoholism and drug usage
  JOURNAL   Ann Agric Environ Med 23 (4), 618-624 (2016)
   PUBMED   28030933
  REMARK    GeneRIF: The present study demonstrated a possible association of
            target P300 evoked theta and of alcohol dependence with SNPs from
            the gene SGIP1 in the region of rs10889635, but further studies are
            required.
REFERENCE   5  (bases 1 to 10737)
  AUTHORS   Shimada,A., Yamaguchi,A. and Kohda,D.
  TITLE     Structural basis for the recognition of two consecutive mutually
            interacting DPF motifs by the SGIP1 mu homology domain
  JOURNAL   Sci Rep 6, 19565 (2016)
   PUBMED   26822536
  REMARK    GeneRIF: crystal structures of the SGIP1 mu homology domains in
            complex with peptides containing two DPF motifs, are reported.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 10737)
  AUTHORS   Luke,M.M., O'Meara,E.S., Rowland,C.M., Shiffman,D., Bare,L.A.,
            Arellano,A.R., Longstreth,W.T. Jr., Lumley,T., Rice,K., Tracy,R.P.,
            Devlin,J.J. and Psaty,B.M.
  TITLE     Gene variants associated with ischemic stroke: the cardiovascular
            health study
  JOURNAL   Stroke 40 (2), 363-368 (2009)
   PUBMED   19023099
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   7  (bases 1 to 10737)
  AUTHORS   Shiffman,D., O'Meara,E.S., Bare,L.A., Rowland,C.M., Louie,J.Z.,
            Arellano,A.R., Lumley,T., Rice,K., Iakoubova,O., Luke,M.M.,
            Young,B.A., Malloy,M.J., Kane,J.P., Ellis,S.G., Tracy,R.P.,
            Devlin,J.J. and Psaty,B.M.
  TITLE     Association of gene variants with incident myocardial infarction in
            the Cardiovascular Health Study
  JOURNAL   Arterioscler Thromb Vasc Biol 28 (1), 173-179 (2008)
   PUBMED   17975119
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   8  (bases 1 to 10737)
  AUTHORS   Uezu,A., Horiuchi,A., Kanda,K., Kikuchi,N., Umeda,K., Tsujita,K.,
            Suetsugu,S., Araki,N., Yamamoto,H., Takenawa,T. and Nakanishi,H.
  TITLE     SGIP1alpha is an endocytic protein that directly interacts with
            phospholipids and Eps15
  JOURNAL   J Biol Chem 282 (36), 26481-26489 (2007)
   PUBMED   17626015
  REMARK    GeneRIF: SGIP1alpha plays an essential role in clathrin-mediated
            endocytosis by interacting with phospholipids and Eps15.
REFERENCE   9  (bases 1 to 10737)
  AUTHORS   Trevaskis,J., Walder,K., Foletta,V., Kerr-Bayles,L., McMillan,J.,
            Cooper,A., Lee,S., Bolton,K., Prior,M., Fahey,R., Whitecross,K.,
            Morton,G.J., Schwartz,M.W. and Collier,G.R.
  TITLE     Src homology 3-domain growth factor receptor-bound 2-like
            (endophilin) interacting protein 1, a novel neuronal protein that
            regulates energy balance
  JOURNAL   Endocrinology 146 (9), 3757-3764 (2005)
   PUBMED   15919751
REFERENCE   10 (bases 1 to 10737)
  AUTHORS   Simpson,J.C., Wellenreuther,R., Poustka,A., Pepperkok,R. and
            Wiemann,S.
  TITLE     Systematic subcellular localization of novel proteins identified by
            large-scale cDNA sequencing
  JOURNAL   EMBO Rep 1 (3), 287-292 (2000)
   PUBMED   11256614
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA491011.1, AL136561.1, BC040516.1, AL354978.20 and AW205180.1.
            
            On Nov 23, 2018 this sequence version replaced NM_032291.3.
            
            Summary: SGIP1 functions as an endocytic protein that affects
            signaling by receptors in neuronal systems involved in energy
            homeostasis via its interaction with endophilins (see SH3GL3; MIM
            603362) (Trevaskis et al., 2005 [PubMed 15919751] and Uezu et al.,
            2007 [PubMed 17626015]).[supplied by OMIM, Mar 2008].
            
            Transcript Variant: This variant (1) encodes isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.9408.1, AL136561.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2145743, SAMEA2146411
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000371037.9/ ENSP00000360076.3
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-129               DA491011.1         198-326
            130-597             AL136561.1         1-468
            598-687             BC040516.1         542-631
            688-4683            AL136561.1         559-4554
            4684-10355          AL354978.20        28989-34660
            10356-10737         AW205180.1         18-399              c
FEATURES             Location/Qualifiers
     source          1..10737
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p31.3"
     gene            1..10737
                     /gene="SGIP1"
                     /note="SH3GL interacting endocytic adaptor 1"
                     /db_xref="GeneID:84251"
                     /db_xref="HGNC:HGNC:25412"
                     /db_xref="MIM:611540"
     exon            1..216
                     /gene="SGIP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             207..2693
                     /gene="SGIP1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     SH3-containing GRB2-like protein 3-interacting protein 1;
                     SH3 domain GRB2 like endophilin interacting protein 1;
                     endophilin-3-interacting protein"
                     /codon_start=1
                     /product="SH3-containing GRB2-like protein 3-interacting
                     protein 1 isoform 1"
                     /protein_id="NP_115667.2"
                     /db_xref="CCDS:CCDS30744.1"
                     /db_xref="GeneID:84251"
                     /db_xref="HGNC:HGNC:25412"
                     /db_xref="MIM:611540"
                     /translation="MMEGLKKRTRKAFGIRKKEKDTDSTGSPDRDGIQPSPHEPPYNS
                     KAECAREGGKKVSKKSNGAPNGFYAEIDWERYNSPELDEEGYSIRPEEPGSTKGKHFY
                     SSSESEEEEESHKKFNIKIKPLQSKDILKNAATVDELKASIGNIALSPSPVRKSPRRS
                     PGAIKRNLSSEEVARPRRSTPTPELISKKPPDDTTALAPLFGPPLESAFDEQKTEVLL
                     DQPEIWGSGQPINPSMESPKLTRPFPTGTPPPLPPKNVPATPPRTGSPLTIGPGNDQS
                     ATEVKIEKLPSINDLDSIFGPVLSPKSVAVNAEEKWVHFSDTSPEHVTPELTPREKVV
                     SPPATPDNPADSPAPGPLGPPGPTGPPGPPGPPRNVLSPLNLEEVQKKVAEQTFIKDD
                     YLETISSPKDFGLGQRATPPPPPPPTYRTVVSSPGPGSGPGPGTTSGASSPARPATPL
                     VPCRSTTPPPPPPRPPSRPKLPPGKPGVGDVSRPFSPPIHSSSPPPIAPLARAESTSS
                     ISSTNSLSAATTPTVENEQPSLVWFDRGKFYLTFEGSSRGPSPLTMGAQDTLPVAAAF
                     TETVNAYFKGADPSKCIVKITGEMVLSFPAGITRHFANNPSPAALTFRVINFSRLEHV
                     LPNPQLLCCDNTQNDANTKEFWVNMPNLMTHLKKVSEQKPQATYYNVDMLKYQVSAQG
                     IQSTPLNLAVNWRCEPSSTDLRIDYKYNTDAMTTAVALNNVQFLVPIDGGVTKLQAVL
                     PPAVWNAEQQRILWKIPDISQKSENGGVGSLLARFQLSEGPSKPSPLVVQFTSEGSTL
                     SGCDIELVGAGYRFSLIKKRFAAGKYLADN"
     exon            217..280
                     /gene="SGIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            281..305
                     /gene="SGIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            306..377
                     /gene="SGIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            378..434
                     /gene="SGIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            435..489
                     /gene="SGIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            490..665
                     /gene="SGIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            666..677
                     /gene="SGIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            678..689
                     /gene="SGIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            690..714
                     /gene="SGIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            715..766
                     /gene="SGIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            767..852
                     /gene="SGIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            853..945
                     /gene="SGIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            946..1020
                     /gene="SGIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1021..1521
                     /gene="SGIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1522..1649
                     /gene="SGIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1650..1776
                     /gene="SGIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1777..1836
                     /gene="SGIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1837..1948
                     /gene="SGIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1949..2104
                     /gene="SGIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2105..2237
                     /gene="SGIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2238..2440
                     /gene="SGIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2441..2505
                     /gene="SGIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2506..2670
                     /gene="SGIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2671..10737
                     /gene="SGIP1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4663..4668
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SGIP1"
                     /note="hexamer: ATTAAA"
     polyA_site      4683
                     /gene="SGIP1"
                     /note="major polyA site"
     regulatory      7878..7883
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SGIP1"
                     /note="hexamer: ATTAAA"
     polyA_site      7898
                     /gene="SGIP1"
                     /note="major polyA site"
     regulatory      8576..8581
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SGIP1"
                     /note="hexamer: AATAAA"
     polyA_site      8606
                     /gene="SGIP1"
                     /note="major polyA site"
     regulatory      10701..10706
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SGIP1"
                     /note="hexamer: AATGAA"
     regulatory      10716..10721
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SGIP1"
                     /note="hexamer: AATAAA"
     polyA_site      10721
                     /gene="SGIP1"
     polyA_site      10737
                     /gene="SGIP1"
ORIGIN      
        1 atcttcttgg tagcctgcct gtaggtgaag aagcaccagc agcatccatg gcctgtcttt
       61 tggcttaaca cttatctcct ttggctttga cagcggacgg aatagacctc agcagcggcg
      121 tggtgaggac ttagctggga cctggaatcg tatcctcctg tgttttttca gactccttgg
      181 aaattaagga atgcaattct gccaccatga tggaaggatt gaaaaaacgt acaaggaagg
      241 cctttggaat acggaagaaa gaaaaggaca ctgattctac aggttcacca gatagagatg
      301 gaattcagcc cagcccacac gaaccaccct acaatagcaa agcagagtgt gcgcgtgaag
      361 gaggaaaaaa agtttcgaag aaaagcaatg gggcaccaaa tggattttat gcggaaattg
      421 attgggaaag atataactca cctgagctgg atgaagaagg ctacagcatc agacccgagg
      481 aacccggctc taccaaagga aagcactttt attcttcaag tgaatcggaa gaagaagaag
      541 aatcacataa gaaatttaat atcaagatta aaccattgca atctaaagac attcttaaga
      601 atgctgcaac tgtagatgaa ttgaaggcat caataggcaa catcgcactt tccccatcac
      661 cagtgaggaa aagtccgagg cgcagcccgg gtgcaattaa aaggaactta tccagtgaag
      721 aagtggcaag acccaggcgt tccacaccaa ctccagaact tataagcaaa aagcctccag
      781 atgacactac ggcccttgct cctctctttg gcccaccact agaatcagct tttgatgaac
      841 agaagacaga agttctttta gatcagcctg agatatgggg ttcaggccaa ccaattaatc
      901 caagcatgga gtcgccaaag ttaacaaggc cttttcccac tggaacacct ccaccactgc
      961 ctccaaaaaa tgtaccagct accccacccc gaacaggatc ccccttaaca attggaccag
     1021 gaaatgacca gtcagccaca gaggtcaaaa ttgaaaaact accatccatc aatgacttgg
     1081 acagcatttt tgggccagta ttgtccccca agtctgttgc tgttaatgct gaagaaaagt
     1141 gggtccattt ttctgataca tccccggaac atgttactcc ggagttgact ccaagggaaa
     1201 aagtggtgtc cccaccagct acaccagaca acccagctga ctccccagct ccaggccctc
     1261 tcggcccccc aggtcccaca ggccccccag ggcctcctgg gcctcctcgc aatgtactat
     1321 cgccgctcaa tttagaagaa gtccagaaga aagtcgctga gcagaccttc attaaagatg
     1381 attacttaga aacaatctca tctcctaaag attttgggtt gggacaaaga gcaactccac
     1441 ctcccccacc accacccacc tacaggactg tggtttcgtc ccccggacct ggctcgggcc
     1501 ctggtccggg gaccaccagt ggtgcatcat cccctgctcg accagccact cctttggttc
     1561 cttgcagaag taccactcca cctccacctc ctccccggcc tccatcccgg ccaaagctac
     1621 ctccaggaaa acctggagtt ggagatgtgt ccagaccttt tagccctccc attcattctt
     1681 ccagccctcc tccaatagca cccttagcgc gggctgaaag cacttcttca atatcgtcaa
     1741 ccaattcctt gagcgcagcc accactccca cagttgagaa tgaacagcct tccctcgttt
     1801 ggtttgacag aggaaagttt tatttgactt ttgaaggttc ttccagggga cccagccccc
     1861 taaccatggg agctcaggac actctccctg ttgcagcagc atttacagaa acagtcaatg
     1921 cctatttcaa aggagcagac ccaagcaaat gtatcgttaa gattaccgga gaaatggtgt
     1981 tgtcatttcc tgctggcatc accagacact ttgccaacaa cccgtcccca gctgctctga
     2041 cttttcgggt gataaatttc agcaggttag aacacgtcct gccaaacccc caacttctct
     2101 gctgtgataa tacacaaaat gatgccaata ccaaggaatt ctgggtaaac atgccaaatt
     2161 tgatgactca cctaaagaaa gtgtctgaac aaaaacccca ggctacatat tataacgttg
     2221 acatgctcaa atatcaggtg tctgcccagg gcattcagtc cacacctctg aacctggcag
     2281 tgaattggcg atgtgagcct tcaagcactg acctgcgcat agattacaaa tataatacag
     2341 atgcaatgac gactgctgtg gccctcaaca atgtgcagtt cctggtcccc atcgacggag
     2401 gagtcaccaa gctccaggca gtgctcccac cagcagtctg gaatgctgaa caacagagaa
     2461 tattgtggaa gattcctgat atctctcaga agtcagaaaa tggaggggtg ggttctttgt
     2521 tggcaagatt tcagttatct gaaggcccaa gcaaaccttc tccattggtt gtgcagttca
     2581 caagtgaagg aagcaccctt tctggctgtg acattgaact tgttggagca gggtatcgat
     2641 tttcactcat caagaaaagg tttgctgcag gaaaatactt ggcagataac taatgaaatc
     2701 ttatgcaagg atttggagga ttcatataat ggagaactga tgtatgagaa acagatttta
     2761 attttggttt gatgaaaaca aaccaatatc tgcacttggg atatatcagg tggaaagtca
     2821 atgactttca tctgtgattt ccctcacaca ctaccatgat gaccagtcct acagtattta
     2881 cttctaggtg taatattgtt aatggtttta aaatgtaatt attgtatttg taaattgtac
     2941 tctcattcca gtaaggcagt tagacacttg agttttagca ttttaccatt cctgaaatgg
     3001 atgtaattta aactgtggta tgtaaattta atagtagtat tgttgaatgg cacaatgctt
     3061 acagaggtag attgcatttt gtcaatatat aaaatttaaa tataatattg atagctgtca
     3121 taaagggggt gccacatatt aaagaaactt aagtggaacc agaagaaaaa gaaacaaact
     3181 tacttttctt caatgcttag tatgttttac tctagtgcta aataaaaact ctatcttcaa
     3241 atgtttagtg ggttaaattg agaaactatt tcagaaaaaa attctaaggt tacagcatat
     3301 tcaaagaaaa gcattagtta ccacttttta aaaagctttt ttttcaaact gcaaatttca
     3361 taaaaatgca aactgtgtaa acagggcctc ttatttttat aacttgtgta aaaagggaaa
     3421 gcaattcata tttaaagttt aagtatatta aattataatc aagagtaaag aagatgttga
     3481 agtcttaact acttgcccct ctctacagtt tcgcaaatgt ggggattgct gaataatcag
     3541 tcagactaaa accaaaattg tgattttaag atttcaagac tttccgtagt tgaactggtt
     3601 aagaattttt gcttagttac tctgaataga tgatcttact catccagtat gggggaatga
     3661 tacctcacgt cttcctcttt acccacagga atcaaaacgc tgagactgag aattttaggg
     3721 aaaaaaaagt ccactgttta gatccagaag gagagtttta atcattgttt atatcatttg
     3781 agaatgaaaa aataagcttc ataaatgaaa ttctattcac attactgtgt aataaatttc
     3841 cttttggatg attaggattc attgtataaa actgtaaatc tttgccattc ttggagaagc
     3901 aaaaggagag ttatcaaaaa tgtatgtcgt ttcatcgttg caaggtataa taaaaactgt
     3961 aattattcaa tctggccctg ccatatgaac atttagaaag acaaacttct tcgggagtct
     4021 cagttgtaaa accttccctc attaatatct gaaaatgtta gtcttccttt aagtcataga
     4081 acttatttaa acataaacca atttctatta caggttatgc tattaaatag ctgtaattat
     4141 taagttatta tttttataat tagttgttaa atttcatttt acacccactc aaatttaaca
     4201 aagaatcttt agccccttta aattttagaa ttaaattaaa tttttaaagt tttacttcta
     4261 aaatgagatt gtgactggca attgtttata gtgaaacttt ttaaattaat ctttgtactc
     4321 ctctatcagt gcttgctacc aagagaatgt ccaaaatgat ttgttttacc atgggaaaat
     4381 tcttactatt caacaaactc tcagttggcc ccctacagca gtctggtgtt gaagtttctt
     4441 tgaacgaact aaatatactc attttatgta aaggtatcca atttgatttt gaaaccaaaa
     4501 tagaaaatgc aaaattctaa attccatgaa acatggaatt tatgacacca aaatcaatgg
     4561 agagtaagca gcagcaaact gagaattatc cagcatatga atataacaat gtgtttttaa
     4621 gtaatcaatt catttaaaaa attgaatatt aatacaaagc atattaaaaa catgtaaata
     4681 ttactggttc tcatgtcttt gtatttatat tttattttgt tttattttgt atagatctag
     4741 aatgaattag taattaaaca caagggtttt tttcccaaaa aataatttta ctgattttta
     4801 aaaatttaat taaaaacata gattacagta atctctaaca ttattcaaat ctttctaggg
     4861 attaaattag aaactatgaa gaaatcctat aatctcttaa ctggtcttta tggaacagaa
     4921 tgggagtcaa caatttcaaa atgattgaac tatgagtaac cagaacctca atagaaaatg
     4981 tatcccttca cctttcagaa aagagatcaa ataataacac aataagctgg tatcagggat
     5041 ttgcattttg acattttgtt gtttcttttt aattttgtag attataatct cctgttacat
     5101 ctatatccac agtactgtag aataagaatt catgtcctga aaatggtccc aggcaatgaa
     5161 agagtctcag ttatcaaaga cattaagtca gattcattta ttcattttat tcccaagtca
     5221 gaacaaaatg aactagtagg taataaaact attttcagta ctaggggtta aaacaaaaag
     5281 aaattgttct agttaaccag aacaccattt cctgaacgtt atgatcttta aaaggttctg
     5341 ctttagcaag ataaaaagta taaatgatgc tactttattt tggcaacatg gaaattttgt
     5401 ttttcttttc caataaaatc catattttcg tgaaattttt aaaaataaat ttgtatcaag
     5461 gattagtctg aattttatca taatagaaat ttataattac taagaattaa atttttaatc
     5521 atttttaaag ctgtgttctt ctctacaccc acagccaaaa tggaaattca ctgtatggta
     5581 tttgggaata tatttcacct gtaaaagaaa gaatgtccac aattcaaacg gttttgagtt
     5641 aatagatagg aatccctcag tcaaactaca gaattttttt tgttttatat attcttagtc
     5701 agcagagtgt ttgagctata attacaagta tttccacttc taaatcttct tttagaaaag
     5761 cagataacta taataccttg gctctgccac tgactcctgc cgtatgatat tggacaaatc
     5821 actgcccttt tcagtttctc atctttagaa tggggagttg ggattgccag tggttttcaa
     5881 gcttttctta acagcagggc tttttttcta ataaaattgc acatggaatc ctggtaggca
     5941 aaacagctca aagcaatgca ggagtttctc caacagttta aattggggct ccccagagca
     6001 tggtttaaat accataacag tacacaatga tctatcatgt gtcttccaac tctgaagttc
     6061 cacagttgta atttactcag ataaaccacc ttttgaggct ttataagcaa acttgtaaaa
     6121 acacagtaat tgaatgatat acaataaaaa ctgacagagt gtttaccatg taccagtcac
     6181 tagagatatt agctcattta attctcttat caatcaaaaa attgtaagta ctctttttaa
     6241 ttcctgtttt acaagtgatg acactgaggc ttaaatagaa taatctcccc aaaatgacac
     6301 ggcagctgaa agtaacccag ctattctgac tgcaaaggaa acgccttaat tgctttgtcc
     6361 accctgaaca aaacaatctg ttttatgtca ctatacgtac tccatttgaa tgccaaggta
     6421 gaaggcacca tgaaaaacca ttaccaaaat aaggataatt ccaataacaa aactgtcaaa
     6481 aaagaatcac tgcttctcaa aatatgattg gtatcatcac ttattaatgt tttgtatcat
     6541 cacttattaa tgattttggc ttggtcacca ctatgcctgg aagatagtag gcactgataa
     6601 acattgttaa acaccgtaac aacacaaaat gatctaccac atatcaatgt cttccatctc
     6661 tgaaattcca tgattctact ttactcagat ataccacctt ttgtggcatt ataaggaaac
     6721 atgtataatc aagttcatac aagctataca cagtaggcat cgataaattt gtattgacag
     6781 cataaacttc tagggatgag atttgggaat atacattttg ataagtgctc caaatttgag
     6841 aatcattgct ctagtagatc tctaaggtcc ttcttagttc tgacattata tcagatttta
     6901 agcattaaat tctaagatga aaaccatttt tctagtcagt ttatttttca ttcagtccac
     6961 ttaatttcct ccaatcttaa aaaatttttt tccctaggaa aatccctatc tttttcttaa
     7021 agttactctg ttatactatc tcagtctcaa agtagcctga aatgcatttt ctcattttca
     7081 tatttctgtc cttttcttca atgtcttaaa agacttgaag tagaagggaa gttgcaagct
     7141 gttatttatt caaatacaaa atcctaatca atgtttcctt ctttgcaagg gacattcaga
     7201 tctctaactt gtgagttgaa cctatttatt ttgtaagctt ttgactttac aaacatgtgg
     7261 tagcctcatt ctaaaagagt tgcacgaata ttttttctca aaataaccac aggctgtaaa
     7321 ttaaattgaa caatacagta gaatatgcag atacatacta aacagatgag tgtgtgtgta
     7381 tgtgtctgtg tttaagcttt tccttttcca aaagattata catttgagca aaatcccatt
     7441 cagtctaaat gtactcattt tgccacatgg ttttctgatc agcaattttt ttaaaaaatg
     7501 gaagtgttac attatattta ataatatcaa aacaagagtg atctctaaat atagctttga
     7561 aagttaatat aatatgatgt gtccatgcaa tgagtcaaat tagattgttg atataaatga
     7621 acaaatcaat ccaagttaca ttgattaaag ggactatttg gatgaccagc ctcaccatct
     7681 tttactgtgt gcactttcct cttaaggttt tattgtattt tccctgctcc tttaggcttt
     7741 tttttcatgt ggaaaacagt atctaaatgc cattctctca cttatgaatt aaatatggct
     7801 accattaatg ttcctttttg tcagtggagt gtatggaagg aaaatgagat aggagtacat
     7861 tgcttaagtc tctaaatatt aaaaacaaca acaaaacact tgcctttctc ttgtgtcatt
     7921 gcttcaatgt tctgtcatct tctgcaagct agagttgcac tgctgacatc taattaagat
     7981 ctaaatatat ttcatgaagg aatgtcaaat gtaaagtctg ttgagttgac catggtgaac
     8041 ctcttgattg gccctcacat ttaatatctg atttctctcc taaatgggta tgtctatgag
     8101 gaaaagacac ctatttgttg gcattgacca tgggtataat aatttattct ctaggtttaa
     8161 cttctaaaat tatttttcct ttgatttcta actgcctcct tctatcccta gagtaatacc
     8221 ttgcctacct taaatacatc tttatccttg taaaaaatgt tttggcaaaa aacaaatata
     8281 tttttaaatg tcccgtaatg ttttaaagaa aagaagtaaa gtgtatttgg gctatgctca
     8341 ccaatattcc agtattttac aaattgtgtt ctggaagatg ttcattgtgg tctgagaaca
     8401 aatggtccta tactataatt ctctctcctt ggacattgac aataaatgtt agtgttttaa
     8461 tgaagcttaa gaaagttatt gaatcaggta aaaaaaattt tttctaagca caccttgaga
     8521 aacctttgga aaagttttct tttaacacta gtttatatat ccttcaataa ttgtaaataa
     8581 aataaccagg aatgctttga aaaaaatgct tctggaattt tgttttaatt ttaagagaat
     8641 tcctgtcatt tcaaatgaat tgttatctat tttaaatttc attagtatct cttagtgaat
     8701 ttaactttat catctaaaat attcaaaaca aaacaataaa attattttaa tttttgtatt
     8761 atttttaagg cttgcaccta tgaccagcat atcagacccc tatctgttga agatgtttcc
     8821 aattctttgc cgtgtggaat taatgccagt gaataatctc cagtaacgtt ttactaaaca
     8881 gctttaggaa actgaatcat gatagatcaa tgttgacagt atttttacat cagcttgtca
     8941 gaaattatat taaaaacact ttggggaaag ctaagagcag tgcactgttt agcacagatt
     9001 ttgtgagatt ctatactctt ttaatgagca tttcataggg tttttttttt gctgtttttt
     9061 tttctttttt tgctttgctt tactttgcca atcagttact tttataccaa attaacgaag
     9121 tttctttaaa aacttcctgg ttaaattgtc tgttatcata tagaaaagaa tcaaactgaa
     9181 tcactttaca tttctctaaa gatagttcag tttttaatcc atccagagtt tgtgaatcag
     9241 cctgatagat cataagccat ttataatttt taaacgttcc attgaatgag tatatcatac
     9301 aaatgttaat tccatctcgc tcattcagtt ttacattact tttatgaatt ttttttctcg
     9361 caaagtaaat gcttgatgtt attcattcac catcactcta aggtatttta aatcatacaa
     9421 caagtaacta tcacaagaac cactgtccca agatccaaag tcagattttt ttcccttttc
     9481 aagttgctag aatgataatt catatagctg tgttgatttt tcaggtcaga atttagttct
     9541 tgctttgtta aatggacata catgaaagag attggctcct atctaattca tattctctct
     9601 ctctctctct ctctttctct gtgtgtgtgt gggggtgtgt gtgtgtgtgt gtgtgtgtgt
     9661 gtgtgtctct ttcctctctg tctaagggga attggaaaat tgctgctgaa taatttttct
     9721 tgaaaaagcc aagtgttgat atgatgatat attaatctta tctatgaaca gagcttcacc
     9781 acaggccaag cagtaatgta tacagctggc tttttaagct gtttgaacac tattgttttc
     9841 gattaaaaag agtttttgtt tgtttttcac ttgtatagta aaagatcata tgaattaaat
     9901 actcggatat taattttcat tcttactaag tggccatgta aggcattgat tttttttaaa
     9961 cttgaacaat gcaagaatct aggaatctca gaaagatttt gctgtgtttc tataggtaat
    10021 gaaatcctgt caattaagga aaatgctgtg tgtaatttga tttggtggta gcctttattt
    10081 agagctctct ttgatctatg tagcaaaaat acactaaatt tctttttaat cttgtactaa
    10141 gtacataatt tcacaaaaat accttgggca taaaatactt acaaaactgt gaattaaaat
    10201 actcaagata ctttcaaaat ttagtcaaaa tgttgattcc cagccttttt cctacacgtc
    10261 tatttcaaaa gagtatactt ttttctgaca gccattactg cttagtctgt aatgaaagct
    10321 ttactgcttc tatgctgtgc tcaagagaaa accaacaacg gaagcgaaga cttacttgct
    10381 ccttcacaga attgcagcaa agctctctgc tgggcaatgg tgacaggaaa gtgtgtaatt
    10441 gacttgttga agataatcat gagaccatct taacattact tgccttataa gttagaaata
    10501 gtatggatat cactctttgt aaataataat ttttgtaaat gttttttgtt cataattttc
    10561 ccaatcacct atttcacaaa atcctgaata tgcaattatt tattatgaca cgttactgca
    10621 actagggctc ccattccaga acaaagttta attctatatg taagttgtta ctgaaatgtg
    10681 atttcttttt ctggaaaata aatgaaaaat atgaaaataa attgaattgt gaatatc
//
