LOCUS       NM_012426               9692 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens splicing factor 3b subunit 3 (SF3B3), mRNA.
ACCESSION   NM_012426
VERSION     NM_012426.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9692)
  AUTHORS   Xu,T., Li,X., Zhao,W., Wang,X., Jin,L., Feng,Z., Li,H., Zhang,M.,
            Tian,Y., Hu,G., Yue,Y., Dai,X., Shan,C., Zhang,W., Zhang,C. and
            Zhang,Y.
  TITLE     SF3B3-regulated mTOR alternative splicing promotes colorectal
            cancer progression and metastasis
  JOURNAL   J Exp Clin Cancer Res 43 (1), 126 (2024)
   PUBMED   38671459
  REMARK    GeneRIF: SF3B3-regulated mTOR alternative splicing promotes
            colorectal cancer progression and metastasis.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9692)
  AUTHORS   Yang,F., Bian,T., Zhan,X., Chen,Z., Xing,Z., Larsen,N.A., Zhang,X.
            and Shi,Y.
  TITLE     Mechanisms of the RNA helicases DDX42 and DDX46 in human U2 snRNP
            assembly
  JOURNAL   Nat Commun 14 (1), 897 (2023)
   PUBMED   36797247
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 9692)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   4  (bases 1 to 9692)
  AUTHORS   Tholen,J., Razew,M., Weis,F. and Galej,W.P.
  TITLE     Structural basis of branch site recognition by the human
            spliceosome
  JOURNAL   Science 375 (6576), 50-57 (2022)
   PUBMED   34822310
REFERENCE   5  (bases 1 to 9692)
  AUTHORS   Will,C.L., Urlaub,H., Achsel,T., Gentzel,M., Wilm,M. and
            Luhrmann,R.
  TITLE     Characterization of novel SF3b and 17S U2 snRNP proteins, including
            a human Prp5p homologue and an SF3b DEAD-box protein
  JOURNAL   EMBO J 21 (18), 4978-4988 (2002)
   PUBMED   12234937
REFERENCE   6  (bases 1 to 9692)
  AUTHORS   Martinez,E., Palhan,V.B., Tjernberg,A., Lymar,E.S., Gamper,A.M.,
            Kundu,T.K., Chait,B.T. and Roeder,R.G.
  TITLE     Human STAGA complex is a chromatin-acetylating transcription
            coactivator that interacts with pre-mRNA splicing and DNA
            damage-binding factors in vivo
  JOURNAL   Mol Cell Biol 21 (20), 6782-6795 (2001)
   PUBMED   11564863
REFERENCE   7  (bases 1 to 9692)
  AUTHORS   Will,C.L., Schneider,C., MacMillan,A.M., Katopodis,N.F.,
            Neubauer,G., Wilm,M., Luhrmann,R. and Query,C.C.
  TITLE     A novel U2 and U11/U12 snRNP protein that associates with the
            pre-mRNA branch site
  JOURNAL   EMBO J 20 (16), 4536-4546 (2001)
   PUBMED   11500380
REFERENCE   8  (bases 1 to 9692)
  AUTHORS   Gangloff,Y.G., Pointud,J.C., Thuault,S., Carre,L., Romier,C.,
            Muratoglu,S., Brand,M., Tora,L., Couderc,J.L. and Davidson,I.
  TITLE     The TFIID components human TAF(II)140 and Drosophila BIP2
            (TAF(II)155) are novel metazoan homologues of yeast TAF(II)47
            containing a histone fold and a PHD finger
  JOURNAL   Mol Cell Biol 21 (15), 5109-5121 (2001)
   PUBMED   11438666
REFERENCE   9  (bases 1 to 9692)
  AUTHORS   Brand,M., Moggs,J.G., Oulad-Abdelghani,M., Lejeune,F.,
            Dilworth,F.J., Stevenin,J., Almouzni,G. and Tora,L.
  TITLE     UV-damaged DNA-binding protein in the TFTC complex links DNA damage
            recognition to nucleosome acetylation
  JOURNAL   EMBO J 20 (12), 3187-3196 (2001)
   PUBMED   11406595
REFERENCE   10 (bases 1 to 9692)
  AUTHORS   Das,B.K., Xia,L., Palandjian,L., Gozani,O., Chyung,Y. and Reed,R.
  TITLE     Characterization of a protein complex containing spliceosomal
            proteins SAPs 49, 130, 145, and 155
  JOURNAL   Mol Cell Biol 19 (10), 6796-6802 (1999)
   PUBMED   10490618
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BI465052.1, BC068974.1,
            AK291768.1, AC106804.4 and AW449427.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_012426.4.
            
            Summary: This gene encodes subunit 3 of the splicing factor 3b
            protein complex. Splicing factor 3b, together with splicing factor
            3a and a 12S RNA unit, forms the U2 small nuclear
            ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a
            complex binds pre-mRNA upstream of the intron's branch site in a
            sequence independent manner and may anchor the U2 snRNP to the
            pre-mRNA. Splicing factor 3b is also a component of the minor
            U12-type spliceosome. Subunit 3 has also been identified as a
            component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase)
            transcription coactivator-HAT (histone acetyltransferase) complex,
            and the TFTC (TATA-binding-protein-free TAF(II)-containing
            complex). These complexes may function in chromatin modification,
            transcription, splicing, and DNA repair. [provided by RefSeq, Jul
            2008].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803614.62084.1,
                                           SRR1803615.266366.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000302516.10/ ENSP00000305790.5
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-35                BI465052.1         34-68
            36-1084             BC068974.1         1-1049
            1085-3170           AK291768.1         1111-3196
            3171-4188           BC068974.1         3136-4153
            4189-9394           AC106804.4         35148-40353         c
            9395-9692           AW449427.1         17-314              c
FEATURES             Location/Qualifiers
     source          1..9692
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16q22.1"
     gene            1..9692
                     /gene="SF3B3"
                     /gene_synonym="RSE1; SAP130; SF3b130; STAF130"
                     /note="splicing factor 3b subunit 3"
                     /db_xref="GeneID:23450"
                     /db_xref="HGNC:HGNC:10770"
                     /db_xref="MIM:605592"
     exon            1..113
                     /gene="SF3B3"
                     /gene_synonym="RSE1; SAP130; SF3b130; STAF130"
                     /inference="alignment:Splign:2.1.0"
     exon            114..253
                     /gene="SF3B3"
                     /gene_synonym="RSE1; SAP130; SF3b130; STAF130"
                     /inference="alignment:Splign:2.1.0"
     CDS             184..3837
                     /gene="SF3B3"
                     /gene_synonym="RSE1; SAP130; SF3b130; STAF130"
                     /note="spliceosome-associated protein 130; pre-mRNA
                     splicing factor SF3b, 130 kDa subunit; SAP 130"
                     /codon_start=1
                     /product="splicing factor 3B subunit 3"
                     /protein_id="NP_036558.3"
                     /db_xref="CCDS:CCDS10894.1"
                     /db_xref="GeneID:23450"
                     /db_xref="HGNC:HGNC:10770"
                     /db_xref="MIM:605592"
                     /translation="MFLYNLTLQRATGISFAIHGNFSGTKQQEIVVSRGKILELLRPD
                     PNTGKVHTLLTVEVFGVIRSLMAFRLTGGTKDYIVVGSDSGRIVILEYQPSKNMFEKI
                     HQETFGKSGCRRIVPGQFLAVDPKGRAVMISAIEKQKLVYILNRDAAARLTISSPLEA
                     HKANTLVYHVVGVDVGFENPMFACLEMDYEEADNDPTGEAAANTQQTLTFYELDLGLN
                     HVVRKYSEPLEEHGNFLITVPGGSDGPSGVLICSENYITYKNFGDQPDIRCPIPRRRN
                     DLDDPERGMIFVCSATHKTKSMFFFLAQTEQGDIFKITLETDEDMVTEIRLKYFDTVP
                     VAAAMCVLKTGFLFVASEFGNHYLYQIAHLGDDDEEPEFSSAMPLEEGDTFFFQPRPL
                     KNLVLVDELDSLSPILFCQIADLANEDTPQLYVACGRGPRSSLRVLRHGLEVSEMAVS
                     ELPGNPNAVWTVRRHIEDEFDAYIIVSFVNATLVLSIGETVEEVTDSGFLGTTPTLSC
                     SLLGDDALVQVYPDGIRHIRADKRVNEWKTPGKKTIVKCAVNQRQVVIALTGGELVYF
                     EMDPSGQLNEYTERKEMSADVVCMSLANVPPGEQRSRFLAVGLVDNTVRIISLDPSDC
                     LQPLSMQALPAQPESLCIVEMGGTEKQDELGERGSIGFLYLNIGLQNGVLLRTVLDPV
                     TGDLSDTRTRYLGSRPVKLFRVRMQGQEAVLAMSSRSWLSYSYQSRFHLTPLSYETLE
                     FASGFASEQCPEGIVAISTNTLRILALEKLGAVFNQVAFPLQYTPRKFVIHPESNNLI
                     IIETDHNAYTEATKAQRKQQMAEEMVEAAGEDERELAAEMAAAFLNENLPESIFGAPK
                     AGNGQWASVIRVMNPIQGNTLDLVQLEQNEAAFSVAVCRFSNTGEDWYVLVGVAKDLI
                     LNPRSVAGGFVYTYKLVNNGEKLEFLHKTPVEEVPAAIAPFQGRVLIGVGKLLRVYDL
                     GKKKLLRKCENKHIANYISGIQTIGHRVIVSDVQESFIWVRYKRNENQLIIFADDTYP
                     RWVTTASLLDYDTVAGADKFGNICVVRLPPNTNDEVDEDPTGNKALWDRGLLNGASQK
                     AEVIMNYHVGETVLSLQKTTLIPGGSESLVYTTLSGGIGILVPFTSHEDHDFFQHVEM
                     HLRSEHPPLCGRDHLSFRSYYFPVKNVIDGDLCEQFNSMEPNKQKNVSEELDRTPPEV
                     SKKLEDIRTRYAF"
     exon            254..580
                     /gene="SF3B3"
                     /gene_synonym="RSE1; SAP130; SF3b130; STAF130"
                     /inference="alignment:Splign:2.1.0"
     exon            581..753
                     /gene="SF3B3"
                     /gene_synonym="RSE1; SAP130; SF3b130; STAF130"
                     /inference="alignment:Splign:2.1.0"
     exon            754..895
                     /gene="SF3B3"
                     /gene_synonym="RSE1; SAP130; SF3b130; STAF130"
                     /inference="alignment:Splign:2.1.0"
     exon            896..1008
                     /gene="SF3B3"
                     /gene_synonym="RSE1; SAP130; SF3b130; STAF130"
                     /inference="alignment:Splign:2.1.0"
     exon            1009..1146
                     /gene="SF3B3"
                     /gene_synonym="RSE1; SAP130; SF3b130; STAF130"
                     /inference="alignment:Splign:2.1.0"
     exon            1147..1250
                     /gene="SF3B3"
                     /gene_synonym="RSE1; SAP130; SF3b130; STAF130"
                     /inference="alignment:Splign:2.1.0"
     exon            1251..1416
                     /gene="SF3B3"
                     /gene_synonym="RSE1; SAP130; SF3b130; STAF130"
                     /inference="alignment:Splign:2.1.0"
     exon            1417..1512
                     /gene="SF3B3"
                     /gene_synonym="RSE1; SAP130; SF3b130; STAF130"
                     /inference="alignment:Splign:2.1.0"
     exon            1513..1585
                     /gene="SF3B3"
                     /gene_synonym="RSE1; SAP130; SF3b130; STAF130"
                     /inference="alignment:Splign:2.1.0"
     exon            1586..1737
                     /gene="SF3B3"
                     /gene_synonym="RSE1; SAP130; SF3b130; STAF130"
                     /inference="alignment:Splign:2.1.0"
     exon            1738..1893
                     /gene="SF3B3"
                     /gene_synonym="RSE1; SAP130; SF3b130; STAF130"
                     /inference="alignment:Splign:2.1.0"
     exon            1894..2049
                     /gene="SF3B3"
                     /gene_synonym="RSE1; SAP130; SF3b130; STAF130"
                     /inference="alignment:Splign:2.1.0"
     exon            2050..2193
                     /gene="SF3B3"
                     /gene_synonym="RSE1; SAP130; SF3b130; STAF130"
                     /inference="alignment:Splign:2.1.0"
     exon            2194..2316
                     /gene="SF3B3"
                     /gene_synonym="RSE1; SAP130; SF3b130; STAF130"
                     /inference="alignment:Splign:2.1.0"
     exon            2317..2471
                     /gene="SF3B3"
                     /gene_synonym="RSE1; SAP130; SF3b130; STAF130"
                     /inference="alignment:Splign:2.1.0"
     exon            2472..2646
                     /gene="SF3B3"
                     /gene_synonym="RSE1; SAP130; SF3b130; STAF130"
                     /inference="alignment:Splign:2.1.0"
     exon            2647..2852
                     /gene="SF3B3"
                     /gene_synonym="RSE1; SAP130; SF3b130; STAF130"
                     /inference="alignment:Splign:2.1.0"
     exon            2853..3009
                     /gene="SF3B3"
                     /gene_synonym="RSE1; SAP130; SF3b130; STAF130"
                     /inference="alignment:Splign:2.1.0"
     exon            3010..3135
                     /gene="SF3B3"
                     /gene_synonym="RSE1; SAP130; SF3b130; STAF130"
                     /inference="alignment:Splign:2.1.0"
     exon            3136..3348
                     /gene="SF3B3"
                     /gene_synonym="RSE1; SAP130; SF3b130; STAF130"
                     /inference="alignment:Splign:2.1.0"
     exon            3349..3447
                     /gene="SF3B3"
                     /gene_synonym="RSE1; SAP130; SF3b130; STAF130"
                     /inference="alignment:Splign:2.1.0"
     exon            3448..3591
                     /gene="SF3B3"
                     /gene_synonym="RSE1; SAP130; SF3b130; STAF130"
                     /inference="alignment:Splign:2.1.0"
     exon            3592..3696
                     /gene="SF3B3"
                     /gene_synonym="RSE1; SAP130; SF3b130; STAF130"
                     /inference="alignment:Splign:2.1.0"
     exon            3697..9692
                     /gene="SF3B3"
                     /gene_synonym="RSE1; SAP130; SF3b130; STAF130"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4298..4303
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SF3B3"
                     /gene_synonym="RSE1; SAP130; SF3b130; STAF130"
                     /note="hexamer: AATAGA"
     polyA_site      4322
                     /gene="SF3B3"
                     /gene_synonym="RSE1; SAP130; SF3b130; STAF130"
                     /note="major polyA site"
     regulatory      9670..9675
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SF3B3"
                     /gene_synonym="RSE1; SAP130; SF3b130; STAF130"
                     /note="hexamer: AATAAA"
     polyA_site      9692
                     /gene="SF3B3"
                     /gene_synonym="RSE1; SAP130; SF3b130; STAF130"
ORIGIN      
        1 gtccctgtct tgaggtctaa tggcggacgc cagtatgttg gagttggtgg tggcttaagt
       61 tttgaaggga ggtagcatcc gttggatatc cacaccatcc ttctcgctgc aggctttctt
      121 ggactccgta ctgttggtgt aaccaaggcc tggaggtctg ggtggctcag gtttcctgca
      181 gccatgtttc tgtacaactt aaccttgcag agagccactg gcatcagctt tgccattcat
      241 ggaaactttt ctggaaccaa acaacaagaa attgttgttt cccgtgggaa gatcttggag
      301 ctgcttcgcc cagaccccaa cactggcaaa gtacataccc tactcactgt ggaagtattc
      361 ggtgttatcc ggtcactcat ggcctttagg ctgacaggtg gcaccaaaga ctacattgta
      421 gttggcagtg actctggtcg aattgttatt ttggaatacc agccatctaa gaatatgttt
      481 gagaagattc accaagaaac ctttggcaag agtggatgcc gtcgcatcgt tcctggccag
      541 ttcttagctg tggatcccaa agggcgagcc gttatgatta gtgccattga gaaacagaaa
      601 ttggtgtata ttttgaacag agatgctgca gcccgactta ccatttcatc tcccctggaa
      661 gcccacaaag caaacacttt agtgtatcat gtagttggag tagatgtcgg atttgaaaat
      721 ccaatgtttg cttgtctgga aatggattat gaggaagcag acaatgatcc aacaggggaa
      781 gcagcagcta atacccagca gacacttact ttctatgagc tagaccttgg tttaaatcat
      841 gtggtccgaa aatacagtga acctttggag gaacacggca acttccttat tacagttcca
      901 ggagggtcag atggtccaag tggagtactg atctgctctg aaaactatat tacttacaag
      961 aactttggtg accagccaga tatccgctgt ccaattccca ggaggcggaa tgacctggat
     1021 gaccctgaaa gaggaatgat ttttgtctgc tctgcaaccc ataaaaccaa atcgatgttc
     1081 ttctttttgg ctcaaactga gcagggagat atctttaaga tcactttgga gacagatgaa
     1141 gatatggtta ctgagatccg gctcaaatat tttgatactg tacccgttgc tgctgccatg
     1201 tgtgtgctta aaacagggtt cctttttgta gcatcagaat ttggaaacca ttacttatat
     1261 caaattgcac atcttggaga tgatgatgaa gaacctgagt tttcatcagc catgcctctg
     1321 gaagaaggag acacattctt ttttcagcca agaccactta aaaaccttgt gctggttgat
     1381 gagttggaca gcctctctcc cattctgttt tgccagatag ctgatctggc caatgaagat
     1441 actccacagt tgtatgtggc ctgtggtagg ggaccccgat catctctgag agtcctaaga
     1501 catggacttg aggtgtcaga aatggctgtt tctgagctac ctggtaaccc caacgctgtc
     1561 tggacagtgc gtcgacacat tgaagatgag tttgatgcct acatcattgt gtctttcgtg
     1621 aatgccaccc tagtgttgtc cattggagaa actgtagaag aagtgactga ctctgggttc
     1681 ctggggacca ccccgacctt gtcctgctcc ttattaggag atgatgcctt ggtgcaggtc
     1741 tatccagatg gcattcggca catacgagca gacaagagag tcaatgagtg gaagacccct
     1801 ggaaagaaaa caattgtgaa gtgtgcagtg aaccagcgac aagtggtgat tgccctgaca
     1861 ggaggagagc tggtctattt cgagatggat ccttcaggac agctgaatga gtacacagaa
     1921 cggaaggaga tgtcagcaga tgtggtgtgc atgagtctgg ccaatgtacc ccctggagag
     1981 cagcggtctc gcttcctggc tgtggggctt gtggacaaca ctgtcagaat catctccctg
     2041 gatccctcag actgtttgca acctctaagc atgcaggctc tcccagccca gcctgagtcc
     2101 ttgtgtatcg tggaaatggg tgggactgag aagcaggatg agctgggtga gaggggctcg
     2161 attggcttcc tatacctgaa tattgggcta cagaacggtg tgctgctgag gactgtcttg
     2221 gaccctgtca ctggggattt gtctgatact cgcactcggt acctggggtc ccgtcctgtg
     2281 aagctcttcc gagtccgaat gcaaggccag gaggcagtat tggccatgtc aagccgctca
     2341 tggttgagct attcttacca atctcgcttc catctcaccc cactgtctta cgagacactg
     2401 gaatttgcat cgggttttgc ctcggaacag tgtcccgagg gcattgtggc catctccacc
     2461 aacaccctac ggattttggc attagagaag ctcggtgctg tcttcaatca agtagccttc
     2521 ccactgcagt acacacccag gaaatttgtc atccaccctg agagtaacaa ccttattatc
     2581 attgaaacgg accacaatgc ctacactgag gccacgaaag ctcagagaaa gcagcagatg
     2641 gcagaggaaa tggtggaagc agcaggggag gatgagcggg agctggccgc agagatggca
     2701 gcagcattcc tcaatgaaaa cctccctgaa tccatctttg gagctcccaa ggctggcaat
     2761 gggcagtggg cctctgtgat ccgagtgatg aatcccattc aagggaacac actggacctt
     2821 gtccagctgg aacagaatga ggcagctttt agtgtggctg tgtgcaggtt ttccaacact
     2881 ggtgaagact ggtatgtgct ggtgggtgtg gccaaggacc tgatactaaa cccccgatct
     2941 gtggcagggg gcttcgtcta tacttacaag cttgtgaaca atggggaaaa actggagttt
     3001 ttgcacaaga ctcctgtgga agaggtccct gctgctattg ccccattcca ggggagggtg
     3061 ttgattggtg tggggaagct gttgcgtgtc tatgacctgg gaaagaagaa gttactccga
     3121 aaatgtgaga ataagcatat tgccaattat atctctggga tccagactat cggacatagg
     3181 gtaattgtat ctgatgtcca agaaagtttc atctgggttc gctacaagcg taatgaaaac
     3241 cagcttatca tctttgctga tgatacctac ccccgatggg tcactacagc cagcctcctg
     3301 gactatgaca ctgtggctgg ggcagacaag tttggcaaca tatgtgtggt gaggctccca
     3361 cctaacacca atgatgaagt agatgaggat cctacaggaa acaaagccct gtgggaccgt
     3421 ggcttgctca atggggcctc ccagaaggca gaggtgatca tgaactacca tgtcggggag
     3481 acggtgctgt ccttgcagaa gaccacgctg atccctggag gctcagaatc acttgtctat
     3541 accaccttgt ctggaggaat tggcatcctt gtgccattca cgtcccatga ggaccatgac
     3601 ttcttccagc atgtggaaat gcacctgcgg tctgaacatc cccctctctg tgggcgggac
     3661 cacctcagct ttcgctccta ctacttccct gtgaagaatg tgattgatgg agacctctgt
     3721 gagcagttca attccatgga acccaacaaa caaaagaacg tctctgaaga actggaccga
     3781 accccacccg aagtgtccaa gaaactcgag gatatccgga cccgctacgc cttctgagcc
     3841 ctcctttccc ggtggggctt gccagagact gtgtgttttg tttcccccac caccatcact
     3901 gccacctggc ttctgccatg tggcaggagg gtgactggat aattaagact gcattatgaa
     3961 agtcaacagc tctttcccct cagctcttct cctggaatga ctggcttccc ctcaaattgg
     4021 cactgagatt tgctacactt ctccccacct ggtacatgat acatgacccc aggttccagt
     4081 gtagaacctg agtcccccat tccccaaagc catccctgca ttgatatgtc ttgactctcc
     4141 tgtctacttt tgcacacacc cttaattttt aattggtttt cttgtaaata cagttttgta
     4201 caatgttatc tctgtgggag gaaggaggca ggctgtggtg ggactgggta gggtatagta
     4261 tcactcctga gttccactgc tctagaatct aaccagaaat agaaacctag tttttaaggt
     4321 gactggcatc catgtgtctt gttctggaga tgaggatgta ggtgggaggt ttgaacccaa
     4381 gttagagcag gaagaactga gtagactcct tccttccaga taccgacttg gacttgcggc
     4441 actctgtggc tccccacccc caggtctgtg gtggtttctt tgttttttcc tggttctttt
     4501 tgctgtgctg atgaaacatg acctcaataa ccatgtgtat acccacccct cttcccactg
     4561 ggtattgagg aagggtggct gattcttcct cctcttctac tctgaggatg ttagtatggg
     4621 gattttagca tgaattctag ctggggagtc ttaacagatg ccccttttac tgatacagca
     4681 cctaaagcga tctttggctc cataggacca taggaagggt cagtacagaa gaacctagat
     4741 actgccctgc ccctgagaac tgtgtatatg tggggcctgt ctgcagcacc catctcaggt
     4801 gggttccaga gggcctttag ggtataatga gagcctgtta ggtggaagag gcccagttcc
     4861 agaaatgttc cagcccaccc ctgagaattc ctcctgttta gttgtgtggg aagccctcgt
     4921 cttccaggct gtccttgcgc cttgaacctg gagaagtgag ctcactgttc tcaatacttc
     4981 acaaatgtaa aactttcttt cgtctgcatg tgctcagcca tctaaattga gcaaatgatc
     5041 tggtgagcac tgggttagaa tcaggaatgg tggaatacaa tctgaacctc tcagagccca
     5101 gaacagaggg ttcctgacac tgtgacactg tctcctggaa ctaagtatct cttgaatcat
     5161 gacttggttt tagatcagtc aagagagacc caggttttgc caggaatcga atccctaaat
     5221 aacatgtttt tttctcactt agctcatgaa tttgcatagt agacagtagt tctgaattag
     5281 attttgaaaa cctaatttca gggctcattt tttcctgtgg ccctaaatcc attctatcaa
     5341 attgtgtgat actgacatgc agtcatctga ggaactcagc gtagatactt gagcagctcc
     5401 tcgcctcttt tctaactcaa gtttgactaa aatacataca ctccgtacag aaggtagggg
     5461 gttatgtaag aaaggaaaac ctaatctatg gaatcaggag ttgtcaccac cgagcttcct
     5521 ctggaagtct gcccatcagc ttgcttgttc tctgttaaga ggaagggcta ggacaaggat
     5581 ttgggcttga atatgtggaa aggaattttc atagttgttg ctgcaggacc tacaaaagtt
     5641 taaaattaga ttggatgtga ctcaatgaca agtcccatct gtgtaattgt taaggggacc
     5701 tgattgactc ctgtggtttg attgagcaac caggtaaata gagacctctc tccagctttg
     5761 gcaaaaccca tcagaggctg ctgcagaact cagacagagg gatctgccct tgggtttgct
     5821 tccatcctgt tccattgcta agcccttgtg acttggatcc taggactgaa aagtttttag
     5881 ctgcctcagc tttcccctga ccttactggc agaggttctg cagatgtttc ctttggaaga
     5941 tctcttgcca agaatagcat tcctttggag gaggggggtt ctagttggaa tgttgctttt
     6001 cttggttagt gtaaatgtat tgctagtgag acagctgccg gcgctggaaa aggctcgtct
     6061 cacagggaga gtgctggtcc ccagaatgtg tgctgttccc acgctgctgc ctttcttgag
     6121 cttgttagag gaaagccaga aaggcattca gatgggatca gtctggcttt caaatttttt
     6181 ttaattccta agttctgttt tattttttaa ttttttaaaa aaaattttat tagagacagt
     6241 ctctctctct tgcctagctg ggagtgcagt ggagtgatca tagctcactg aggcttgaac
     6301 tcctgggctc gagcaatcca cctcagcctc cagagtaggg gagactacag atgtgtgcca
     6361 ccatactcag ctaattttta aacttttgta gagacagggt ctccctgtgt tgcccaggct
     6421 ggcctcgaac tcctgacctc aaaaaatctt cctgccttgg cctcccaaag cactgggatt
     6481 ataggtgtga gccattgcgc ctggtcataa attcttgttt ttttgtttgt ttgtttatta
     6541 gagatggaat ctctctctct tgaccaggct agagggctgt ggtgcgatct cagcccactg
     6601 caacctctat ctcctgagct caagcgatcc tccttagctt cccaaatagc tggaactaca
     6661 ggcatgtgcc atcacgtcca gctaattttt gtatttttag tagagaaggt tttaccatgt
     6721 tggacagggt ggtctcgaac tcctggccta aagtggtcca cctagctcag cctaccaaag
     6781 tgctgtgatt acaggcgtga gccaccatgc ccagcctcta aattctgttt tctattcaaa
     6841 gtaaaaatga catgtgtttg agtcatccac ttgctcattt gcatatggaa tattgtatgt
     6901 gaatatttta ttacaccctt aaaattaatt ttcaagggct atatgtagag ctcagaagaa
     6961 acatctctgc tgctgaaatc aaacctggtc caaaaaacgt cacaacggag gaaaggaata
     7021 gatggctctc gaggacaaga tagggactct taaaacacct gcttggccat ggttgattga
     7081 catttgagtg atctggtagc ccaacacacc ctgtgaagtt caggtgaact gaaatgggca
     7141 ggtgtaggct acacaccagt ctgaaccagc acaaccaaga gttgtttctc ttttttttct
     7201 ttttcttttt cttttttttc tttttttttt tttttttttt gagatgaagt cttactctgt
     7261 tgcccaggct agagtacaga ggcacaatct cggctcacca cagcctccgc ctcctgggtt
     7321 caagcaattc tcctgcctca gcctcccaag tagctgggac tacaggcatg caccaccatg
     7381 cctggctaat ttttgtattt ttagtagaga tggggtttca ccatgttggc caggctggtt
     7441 tcgaactcgt gacctcaggt gatccaccca ccttggtctc ccaaagcgct gggattacag
     7501 gcgtgagcca ctgcacttgg ccaggagctg tttaagccat caggagacat gctgacccac
     7561 tagaagaggc tgctacgctc atttcttcca caaacgttta ctgggcacct accatgaacc
     7621 accacctccc agctctgttc tgcacccaga ggttggagga gagtttataa ccaggcagtc
     7681 tctgtcgctg tgaggttggt ggtgaggtga gtctgccaga gggcctgcca ccagtttcag
     7741 aagagcctgc agcctgcatc tgatttcctc agccacaacc ttgggacttc aggaaaaatc
     7801 agaaggtccc aagtctgaaa aatgaagagg tggggagtgg gcagcagggg ttttaggtca
     7861 ggaaatggta ggaaacctgt agttgtggct cataatccta gtgggatatt agaaccctct
     7921 tcatttgtca gaacagattc ctggggtctg ctcccaatta ttcacacggt ggttcatgcc
     7981 tggtaatccc agcactttgg gcggccagag gcaggcagat tgcttgagcc cagaagttta
     8041 agaccagcct aggaaacaga ccccatctct acaaaaaatt caaaaagccg ggcgtgaagc
     8101 cctgtttttt tttttttaaa aaaagagtct gctggtgatg tctgggcatg tgtctgcttc
     8161 acagtccagt gttatgatca gtagctgtga tggacaatga acattgctgc ttgttcaaaa
     8221 tgaagaaaaa aaatatcaaa gaaaaccagg tttggaagtt tcagatttgg aaaaggagct
     8281 gggccagccc atctggaata gctgaggggc cctgttagcc ccaccctacc ccaccccacc
     8341 cccgtatgca gcatctctgg cggtgcccag aggcaagccc ccttcagttt cccacctctc
     8401 actctgcctt ccagagccta gtcaagtttt aaattcccag gtccactcag cagaacttgc
     8461 tcccttctct gactgcagtc tcataatgaa aggagaggtg ctttcagttg ggtcatttgg
     8521 tagcgtttag gcttagaagt tactaatttg gagccgtgag tatatgtgta taagctagta
     8581 acacgtgatg gtcaagggaa gtactaccat ttcctgtggg ttattagggc ctccagctgt
     8641 tatgaggaca cactggggaa tctcagcttt agggagtcga tgatgtaact ggagaaaggc
     8701 aatgctgccc tcataaagca atcagaaacg agattccacc tgccaaatgc caagaggcag
     8761 caaagtccat gaagagagca ctgtatacag tcagatgacc tgggctcact agcctctaag
     8821 catagtcttg gtttcctgcc tgtaaattgg tagaataaga ctgatttact gggcctggca
     8881 tggtggctca cacctgtaat cccagcactt tgagaggctg aggcaggagg atcccttgag
     8941 cccaggagtt tgagaccagc ctgggcaaca tgacaaaacc ccatctctcc aaaaatacaa
     9001 aaattagcca ggcatggtgg tgcacacttg tagtcccagt aattaggggg ctgagacagg
     9061 aggatcactt cagcctatga gtttgaggct gcagtgagct gtgattgcgc cactacactc
     9121 cagcctggat gacaggacga aacctgtctc aaaaacacca aaaaacaaaa accggtctcc
     9181 tggggtcatg gtagcacaaa cgcacatgac tgagtgctca ggggttctga ggcttgtccg
     9241 ctgacctggg gctctggccc tgggagatct gggggacctg ctgtcctata tgtgatgctt
     9301 tgaaagaaag gggcatcatt ccaagccaag aggccccaga gagggcaccg tgcggtgttc
     9361 aggcttctgt gaggccccag tgagatcctg tggctgtgcc cccatcacct ccacccactc
     9421 tgccctccca ctagctgccc aacggatgaa tcaacgcctt ggcagagttt tccagcaggg
     9481 ccttgcagag agtgtgtgtg acctgtgtgg ccactgcctt ggggacgggt gaggagttag
     9541 cctggaacat tccagcgtgg gcattattgt cctgttgcaa gttcagggca aaaccaggaa
     9601 tccagttttg tcgatccaat tgagaaaaca tttcatgaac aactacttgt ggcatgcatt
     9661 ggcactcgga ataaagcgca ctattgtcac ta
//
