LOCUS       NM_007165               1619 bp    mRNA    linear   PRI 20-FEB-2025
DEFINITION  Homo sapiens splicing factor 3a subunit 2 (SF3A2), mRNA.
ACCESSION   NM_007165
VERSION     NM_007165.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Yang,F., Bian,T., Zhan,X., Chen,Z., Xing,Z., Larsen,N.A., Zhang,X.
            and Shi,Y.
  TITLE     Mechanisms of the RNA helicases DDX42 and DDX46 in human U2 snRNP
            assembly
  JOURNAL   Nat Commun 14 (1), 897 (2023)
   PUBMED   36797247
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 1619)
  AUTHORS   Tholen,J., Razew,M., Weis,F. and Galej,W.P.
  TITLE     Structural basis of branch site recognition by the human
            spliceosome
  JOURNAL   Science 375 (6576), 50-57 (2022)
   PUBMED   34822310
REFERENCE   4  (bases 1 to 1619)
  AUTHORS   Qin,Y., Huttlin,E.L., Winsnes,C.F., Gosztyla,M.L., Wacheul,L.,
            Kelly,M.R., Blue,S.M., Zheng,F., Chen,M., Schaffer,L.V., Licon,K.,
            Backstrom,A., Vaites,L.P., Lee,J.J., Ouyang,W., Liu,S.N., Zhang,T.,
            Silva,E., Park,J., Pitea,A., Kreisberg,J.F., Gygi,S.P., Ma,J.,
            Harper,J.W., Yeo,G.W., Lafontaine,D.L.J., Lundberg,E. and Ideker,T.
  TITLE     A multi-scale map of cell structure fusing protein images and
            interactions
  JOURNAL   Nature 600 (7889), 536-542 (2021)
   PUBMED   34819669
REFERENCE   5  (bases 1 to 1619)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   6  (bases 1 to 1619)
  AUTHORS   Moraitou,M., Patrinou-Georgoula,M. and Guialis,A.
  TITLE     Structural/functional properties of a mammalian multi-component
            structure containing all major spliceosomal small nuclear
            ribonucleoprotein particles
  JOURNAL   Biochem J 332 (Pt 1) (Pt 1), 135-144 (1998)
   PUBMED   9576861
REFERENCE   7  (bases 1 to 1619)
  AUTHORS   Hong,W., Bennett,M., Xiao,Y., Feld Kramer,R., Wang,C. and Reed,R.
  TITLE     Association of U2 snRNP with the spliceosomal complex E
  JOURNAL   Nucleic Acids Res 25 (2), 354-361 (1997)
   PUBMED   9016565
REFERENCE   8  (bases 1 to 1619)
  AUTHORS   Rain,J.C., Tartakoff,A.M., Kramer,A. and Legrain,P.
  TITLE     Essential domains of the PRP21 splicing factor are implicated in
            the binding to PRP9 and PRP11 proteins and are conserved through
            evolution
  JOURNAL   RNA 2 (6), 535-550 (1996)
   PUBMED   8718683
REFERENCE   9  (bases 1 to 1619)
  AUTHORS   Dresser,D.W., Hacker,A., Lovell-Badge,R. and Guerrier,D.
  TITLE     The genes for a spliceosome protein (SAP62) and the anti-Mullerian
            hormone (AMH) are contiguous
  JOURNAL   Hum Mol Genet 4 (9), 1613-1618 (1995)
   PUBMED   8541848
REFERENCE   10 (bases 1 to 1619)
  AUTHORS   Bennett,M. and Reed,R.
  TITLE     Correspondence between a mammalian spliceosome component and an
            essential yeast splicing factor
  JOURNAL   Science 262 (5130), 105-108 (1993)
   PUBMED   8211113
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BG325385.1, BC015804.1 and
            BC009903.2.
            
            On Nov 23, 2018 this sequence version replaced NM_007165.4.
            
            Summary: This gene encodes subunit 2 of the splicing factor 3a
            protein complex. The splicing factor 3a heterotrimer includes
            subunits 1, 2 and 3 and is necessary for the in vitro conversion of
            15S U2 snRNP into an active 17S particle that performs pre-mRNA
            splicing. Subunit 2 interacts with subunit 1 through its
            amino-terminus while the single zinc finger domain of subunit 2
            plays a role in its binding to the 15S U2 snRNP. Subunit 2 may also
            function independently of its RNA splicing function as a
            microtubule-binding protein. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC009903.2, SRR3476690.238362.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000221494.10/ ENSP00000221494.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-67                BG325385.1         302-368
            68-1502             BC015804.1         28-1462
            1503-1619           BC009903.2         1503-1619
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19p13.3"
     gene            1..1619
                     /gene="SF3A2"
                     /gene_synonym="PRP11; PRPF11; SAP62; SF3a66"
                     /note="splicing factor 3a subunit 2"
                     /db_xref="GeneID:8175"
                     /db_xref="HGNC:HGNC:10766"
                     /db_xref="MIM:600796"
     exon            1..78
                     /gene="SF3A2"
                     /gene_synonym="PRP11; PRPF11; SAP62; SF3a66"
                     /inference="alignment:Splign:2.1.0"
     exon            79..241
                     /gene="SF3A2"
                     /gene_synonym="PRP11; PRPF11; SAP62; SF3a66"
                     /inference="alignment:Splign:2.1.0"
     CDS             116..1510
                     /gene="SF3A2"
                     /gene_synonym="PRP11; PRPF11; SAP62; SF3a66"
                     /note="pre-mRNA splicing factor SF3A, subunit 2; splicing
                     factor 3a, subunit 2, 66kD; spliceosome associated protein
                     62; SAP 62; splicing factor 3a, subunit 2, 66kDa"
                     /codon_start=1
                     /product="splicing factor 3A subunit 2"
                     /protein_id="NP_009096.2"
                     /db_xref="CCDS:CCDS12084.1"
                     /db_xref="GeneID:8175"
                     /db_xref="HGNC:HGNC:10766"
                     /db_xref="MIM:600796"
                     /translation="MDFQHRPGGKTGSGGVASSSESNRDRRERLRQLALETIDINKDP
                     YFMKNHLGSYECKLCLTLHNNEGSYLAHTQGKKHQTNLARRAAKEAKEAPAQPAPEKV
                     KVEVKKFVKIGRPGYKVTKQRDSEMGQQSLLFQIDYPEIAEGIMPRHRFMSAYEQRIE
                     PPDRRWQYLLMAAEPYETIAFKVPSREIDKAEGKFWTHWNRETKQFFLQFHFKMEKPP
                     APPSLPAGPPGVKRPPPPLMNGLPPRPPLPESLPPPPPGGLPLPPMPPTGPAPSGPPG
                     PPQLPPPAPGVHPPAPVVHPPASGVHPPAPGVHPPAPGVHPPAPGVHPPTSGVHPPAP
                     GVHPPAPGVHPPAPGVHPPAPGVHPPAPGVHPPPSAGVHPQAPGVHPAAPAVHPQAPG
                     VHPPAPGMHPQAPGVHPQPPGVHPSAPGVHPQPPGVHPSNPGVHPPTPMPPMLRPPLP
                     SEGPGNIPPPPPTN"
     exon            242..313
                     /gene="SF3A2"
                     /gene_synonym="PRP11; PRPF11; SAP62; SF3a66"
                     /inference="alignment:Splign:2.1.0"
     exon            314..360
                     /gene="SF3A2"
                     /gene_synonym="PRP11; PRPF11; SAP62; SF3a66"
                     /inference="alignment:Splign:2.1.0"
     exon            361..470
                     /gene="SF3A2"
                     /gene_synonym="PRP11; PRPF11; SAP62; SF3a66"
                     /inference="alignment:Splign:2.1.0"
     exon            471..520
                     /gene="SF3A2"
                     /gene_synonym="PRP11; PRPF11; SAP62; SF3a66"
                     /inference="alignment:Splign:2.1.0"
     exon            521..661
                     /gene="SF3A2"
                     /gene_synonym="PRP11; PRPF11; SAP62; SF3a66"
                     /inference="alignment:Splign:2.1.0"
     exon            662..730
                     /gene="SF3A2"
                     /gene_synonym="PRP11; PRPF11; SAP62; SF3a66"
                     /inference="alignment:Splign:2.1.0"
     exon            731..1619
                     /gene="SF3A2"
                     /gene_synonym="PRP11; PRPF11; SAP62; SF3a66"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1602..1607
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SF3A2"
                     /gene_synonym="PRP11; PRPF11; SAP62; SF3a66"
                     /note="hexamer: ATTAAA"
     polyA_site      1619
                     /gene="SF3A2"
                     /gene_synonym="PRP11; PRPF11; SAP62; SF3a66"
                     /note="major polyA site"
ORIGIN      
        1 ctttctttgc ccgccgttcg ccaaacgaag tcgtggaggt ggcgaaacga ggaggagata
       61 acgcggcctt gggctctggt gtctcccagt ctgctaaagc cctaaggcca tcaccatgga
      121 cttccagcat cgccccgggg gcaagaccgg gagcgggggc gtggcctcct cctccgagag
      181 caaccgtgac cgcagggagc gcctccggca gctggccctg gagaccatcg acatcaacaa
      241 ggacccgtac ttcatgaaga accacctggg ctcctatgaa tgcaaactct gcctgacact
      301 tcacaacaat gaggggagct acctggcaca tacgcagggg aagaagcacc agaccaacct
      361 ggcccggcga gcagccaagg aggccaagga ggcccctgcc cagcccgcgc ctgagaaggt
      421 caaggtggag gtgaagaagt ttgtgaagat cggccgcccg ggctacaaag tgaccaagca
      481 gagagactcg gagatgggcc agcagagcct cctcttccag attgactacc ctgagatcgc
      541 cgagggcatc atgccacgtc accgcttcat gtctgcgtac gagcagagga tcgagcctcc
      601 ggaccggcgc tggcagtacc tgctcatggc cgccgagccc tacgagacca ttgccttcaa
      661 ggtgccgagc agagagatcg acaaggcgga gggcaagttc tggacacact ggaaccggga
      721 gaccaagcag ttcttcctcc agttccactt taagatggag aagcccccgg ctccacccag
      781 cctccctgct ggcccccctg gggtgaagcg gcctccaccc ccgctgatga acggtctgcc
      841 ccctcggcca ccgctgcctg agtctttgcc accgcccccg ccaggaggcc tgcctctgcc
      901 acccatgccc cccacagggc ctgcgccctc agggcccccg ggaccacccc agctaccccc
      961 gccagctcca ggggtccacc ccccggcccc agtggtgcat ccccctgcat ctggggtcca
     1021 tcccccagct cctggcgtcc accccccagc tcctggcgtc catcccccag cccctggggt
     1081 ccacccacca acctctgggg tccacccccc agctcctgga gtccaccctc cagcccccgg
     1141 ggttcaccca ccagcccccg gagtccaccc accagcccct ggggttcacc caccagcccc
     1201 aggggtccat cctcccccat cagcgggggt tcacccccag gccccggggg tgcacccagc
     1261 agcccccgcc gttcaccctc aggccccagg ggtgcaccca ccagccccag ggatgcaccc
     1321 tcaggccccg ggggtccacc cccaacctcc cggggtccat ccgtcggctc ctggggtcca
     1381 ccctcagcct ccgggagttc acccctcaaa tcctggggtg caccccccaa ctcccatgcc
     1441 cccaatgctg aggcccccac ttccctccga aggcccaggg aacatacctc cccctccccc
     1501 aaccaactga gaagctgctc cctcccccag caagcccagc gccaggtgct cttgcctttt
     1561 cccactgaga gaaggctgct cttttgtact gccccccgct cattaaacag cctccccca
//
