LOCUS       NM_015046              11101 bp    mRNA    linear   PRI 10-DEC-2024
DEFINITION  Homo sapiens senataxin (SETX), transcript variant 1, mRNA.
ACCESSION   NM_015046
VERSION     NM_015046.7
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 11101)
  AUTHORS   Zannini,L., Cardano,M., Liberi,G. and Buscemi,G.
  TITLE     R-loops and impaired autophagy trigger cGAS-dependent inflammation
            via micronuclei formation in Senataxin-deficient cells
  JOURNAL   Cell Mol Life Sci 81 (1), 339 (2024)
   PUBMED   39120648
  REMARK    GeneRIF: R-loops and impaired autophagy trigger cGAS-dependent
            inflammation via micronuclei formation in Senataxin-deficient
            cells.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11101)
  AUTHORS   Wen,X., Xu,H., Woolley,P.R., Conway,O.M., Yao,J., Matouschek,A.,
            Lambowitz,A.M. and Paull,T.T.
  TITLE     Senataxin deficiency disrupts proteostasis through nucleolar
            ncRNA-driven protein aggregation
  JOURNAL   J Cell Biol 223 (7) (2024)
   PUBMED   38717338
  REMARK    GeneRIF: Senataxin deficiency disrupts proteostasis through
            nucleolar ncRNA-driven protein aggregation.
REFERENCE   3  (bases 1 to 11101)
  AUTHORS   Appel,C.D., Bermek,O., Dandey,V.P., Wood,M., Viverette,E.,
            Williams,J.G., Bouvette,J., Riccio,A.A., Krahn,J.M., Borgnia,M.J.
            and Williams,R.S.
  TITLE     Sen1 architecture: RNA-DNA hybrid resolution, autoregulation, and
            insights into SETX inactivation in AOA2
  JOURNAL   Mol Cell 83 (20), 3692-3706 (2023)
   PUBMED   37832548
  REMARK    GeneRIF: Sen1 architecture: RNA-DNA hybrid resolution,
            autoregulation, and insights into SETX inactivation in AOA2.
REFERENCE   4  (bases 1 to 11101)
  AUTHORS   Jeridi,C., Rachdi,A., Nabli,F., Saied,Z., Zouari,R., Ben
            Mohamed,D., Ben Said,M., Masmoudi,S., Ben Sassi,S. and Amouri,R.
  TITLE     Genetic heterogeneity within a consanguineous family involving TTPA
            and SETX genes
  JOURNAL   J Neurogenet 37 (4), 124-130 (2023)
   PUBMED   38109176
  REMARK    GeneRIF: Genetic heterogeneity within a consanguineous family
            involving TTPA and SETX genes.
REFERENCE   5  (bases 1 to 11101)
  AUTHORS   Moreira,M.C., Klur,S., Watanabe,M., Nemeth,A.H., Le Ber,I.,
            Moniz,J.C., Tranchant,C., Aubourg,P., Tazir,M., Schols,L.,
            Pandolfo,M., Schulz,J.B., Pouget,J., Calvas,P., Shizuka-Ikeda,M.,
            Shoji,M., Tanaka,M., Izatt,L., Shaw,C.E., M'Zahem,A., Dunne,E.,
            Bomont,P., Benhassine,T., Bouslam,N., Stevanin,G., Brice,A.,
            Guimaraes,J., Mendonca,P., Barbot,C., Coutinho,P., Sequeiros,J.,
            Durr,A., Warter,J.M. and Koenig,M.
  TITLE     Senataxin, the ortholog of a yeast RNA helicase, is mutant in
            ataxia-ocular apraxia 2
  JOURNAL   Nat Genet 36 (3), 225-227 (2004)
   PUBMED   14770181
  REMARK    GeneRIF: Ten of the fifteen mutations cause premature termination
            of a large DEAxQ-box helicase, the human ortholog of yeast Sen1p,
            involved in RNA maturation and termination.
REFERENCE   6  (bases 1 to 11101)
  AUTHORS   Nemeth,A.H., Bochukova,E., Dunne,E., Huson,S.M., Elston,J.,
            Hannan,M.A., Jackson,M., Chapman,C.J. and Taylor,A.M.
  TITLE     Autosomal recessive cerebellar ataxia with oculomotor apraxia
            (ataxia-telangiectasia-like syndrome) is linked to chromosome 9q34
  JOURNAL   Am J Hum Genet 67 (5), 1320-1326 (2000)
   PUBMED   11022012
REFERENCE   7  (bases 1 to 11101)
  AUTHORS   Chance,P.F., Rabin,B.A., Ryan,S.G., Ding,Y., Scavina,M., Crain,B.,
            Griffin,J.W. and Cornblath,D.R.
  TITLE     Linkage of the gene for an autosomal dominant form of juvenile
            amyotrophic lateral sclerosis to chromosome 9q34
  JOURNAL   Am J Hum Genet 62 (3), 633-640 (1998)
   PUBMED   9497266
REFERENCE   8  (bases 1 to 11101)
  AUTHORS   Siddique,N. and Siddique,T.
  TITLE     Amyotrophic Lateral Sclerosis Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301623
REFERENCE   9  (bases 1 to 11101)
  AUTHORS   Moreira,M.C. and Koenig,M.
  TITLE     Ataxia with Oculomotor Apraxia Type 2
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301333
REFERENCE   10 (bases 1 to 11101)
  AUTHORS   Perlman,S.
  TITLE     Hereditary Ataxia Overview
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301317
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA989674.1, AY362728.1,
            BX538166.1, AB014525.2, AA609547.1 and AI123313.1.
            
            On Nov 23, 2018 this sequence version replaced NM_015046.6.
            
            Summary: This gene encodes a protein named for its homology to the
            Sen1p protein of fungi which has RNA helicase activity encoded by a
            domain at the C-terminal end of the protein. The protein encoded by
            this gene contains a DNA/RNA helicase domain at its C-terminal end
            which suggests that it may be involved in both DNA and RNA
            processing. Mutations in this gene have been associated with
            ataxia-ocular apraxia-2 (AOA2) and an autosomal dominant form of
            juvenile amyotrophic lateral sclerosis (ALS4). [provided by RefSeq,
            Jul 2008].
            
            Transcript Variant: This variant (1) represents the shortest
            transcript and encodes the shorter isoform (1). Variants 1 and 2
            encode the same isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BX538166.1, BX537849.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000224140.6/ ENSP00000224140.5
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-548               DA989674.1         1-548
            549-3638            AY362728.1         372-3461
            3639-3759           BX538166.1         3641-3761
            3760-5782           AY362728.1         3583-5605
            5783-8561           BX538166.1         5785-8563
            8562-10715          AB014525.2         8338-10491
            10716-11076         AA609547.1         28-388              c
            11077-11101         AI123313.1         5-29                c
FEATURES             Location/Qualifiers
     source          1..11101
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q34.13"
     gene            1..11101
                     /gene="SETX"
                     /gene_synonym="ALS4; AOA2; bA479K20.2; SCAN2; SCAR1; Sen1;
                     STEX"
                     /note="senataxin"
                     /db_xref="GeneID:23064"
                     /db_xref="HGNC:HGNC:445"
                     /db_xref="MIM:608465"
     exon            1..70
                     /gene="SETX"
                     /gene_synonym="ALS4; AOA2; bA479K20.2; SCAN2; SCAR1; Sen1;
                     STEX"
                     /inference="alignment:Splign:2.1.0"
     exon            71..177
                     /gene="SETX"
                     /gene_synonym="ALS4; AOA2; bA479K20.2; SCAN2; SCAR1; Sen1;
                     STEX"
                     /inference="alignment:Splign:2.1.0"
     exon            178..361
                     /gene="SETX"
                     /gene_synonym="ALS4; AOA2; bA479K20.2; SCAN2; SCAR1; Sen1;
                     STEX"
                     /inference="alignment:Splign:2.1.0"
     CDS             185..8218
                     /gene="SETX"
                     /gene_synonym="ALS4; AOA2; bA479K20.2; SCAN2; SCAR1; Sen1;
                     STEX"
                     /EC_number="3.6.1.-"
                     /note="isoform 1 is encoded by transcript variant 1;
                     probable helicase senataxin; SEN1 homolog; amyotrophic
                     lateral sclerosis 4 protein"
                     /codon_start=1
                     /product="probable helicase senataxin isoform 1"
                     /protein_id="NP_055861.3"
                     /db_xref="CCDS:CCDS6947.1"
                     /db_xref="GeneID:23064"
                     /db_xref="HGNC:HGNC:445"
                     /db_xref="MIM:608465"
                     /translation="MSTCCWCTPGGASTIDFLKRYASNTPSGEFQTADEDLCYCLECV
                     AEYHKARDELPFLHEVLWELETLRLINHFEKSMKAEIGDDDELYIVDNNGEMPLFDIT
                     GQDFENKLRVPLLEILKYPYLLLHERVNELCVEALCRMEQANCSFQVFDKHPGIYLFL
                     VHPNEMVRRWAILTARNLGKVDRDDYYDLQEVLLCLFKVIELGLLESPDIYTSSVLEK
                     GKLILLPSHMYDTTNYKSYWLGICMLLTILEEQAMDSLLLGSDKQNDFMQSILHTMER
                     EADDDSVDPFWPALHCFMVILDRLGSKVWGQLMDPIVAFQTIINNASYNREIRHIRNS
                     SVRTKLEPESYLDDMVTCSQIVYNYNPEKTKKDSGWRTAICPDYCPNMYEEMETLASV
                     LQSDIGQDMRVHNSTFLWFIPFVQSLMDLKDLGVAYIAQVVNHLYSEVKEVLNQTDAV
                     CDKVTEFFLLILVSVIELHRNKKCLHLLWVSSQQWVEAVVKCAKLPTTAFTRSSEKSS
                     GNCSKGTAMISSLSLHSMPSNSVQLAYVQLIRSLLKEGYQLGQQSLCKRFWDKLNLFL
                     RGNLSLGWQLTSQETHELQSCLKQIIRNIKFKAPPCNTFVDLTSACKISPASYNKEES
                     EQMGKTSRKDMHCLEASSPTFSKEPMKVQDSVLIKADNTIEGDNNEQNYIKDVKLEDH
                     LLAGSCLKQSSKNIFTERAEDQIKISTRKQKSVKEISSYTPKDCTSRNGPERGCDRGI
                     IVSTRLLTDSSTDALEKVSTSNEDFSLKDDALAKTSKRKTKVQKDEICAKLSHVIKKQ
                     HRKSTLVDNTINLDENLTVSNIESFYSRKDTGVQKGDGFIHNLSLDPSGVLDDKNGEQ
                     KSQNNVLPKEKQLKNEELVIFSFHENNCKIQEFHVDGKELIPFTEMTNASEKKSSPFK
                     DLMTVPESRDEEMSNSTSVIYSNLTREQAPDISPKSDTLTDSQIDRDLHKLSLLAQAS
                     VITFPSDSPQNSSQLQRKVKEDKRCFTANQNNVGDTSRGQVIIISDSDDDDDERILSL
                     EKLTKQDKICLEREHPEQHVSTVNSKEEKNPVKEEKTETLFQFEESDSQCFEFESSSE
                     VFSVWQDHPDDNNSVQDGEKKCLAPIANTTNGQGCTDYVSEVVKKGAEGIEEHTRPRS
                     ISVEEFCEIEVKKPKRKRSEKPMAEDPVRPSSSVRNEGQSDTNKRDLVGNDFKSIDRR
                     TSTPNSRIQRATTVSQKKSSKLCTCTEPIRKVPVSKTPKKTHSDAKKGQNRSSNYLSC
                     RTTPAIVPPKKFRQCPEPTSTAEKLGLKKGPRKAYELSQRSLDYVAQLRDHGKTVGVV
                     DTRKKTKLISPQNLSVRNNKKLLTSQELQMQRQIRPKSQKNRRRLSDCESTDVKRAGS
                     HTAQNSDIFVPESDRSDYNCTGGTEVLANSNRKQLIKCMPSEPETIKAKHGSPATDDA
                     CPLNQCDSVVLNGTVPTNEVIVSTSEDPLGGGDPTARHIEMAALKEGEPDSSSDAEED
                     NLFLTQNDPEDMDLCSQMENDNYKLIELIHGKDTVEVEEDSVSRPQLESLSGTKCKYK
                     DCLETTKNQGEYCPKHSEVKAADEDVFRKPGLPPPASKPLRPTTKIFSSKSTSRIAGL
                     SKSLETSSALSPSLKNKSKGIQSILKVPQPVPLIAQKPVGEMKNSCNVLHPQSPNNSN
                     RQGCKVPFGESKYFPSSSPVNILLSSQSVSDTFVKEVLKWKYEMFLNFGQCGPPASLC
                     QSISRPVPVRFHNYGDYFNVFFPLMVLNTFETVAQEWLNSPNRENFYQLQVRKFPADY
                     IKYWEFAVYLEECELAKQLYPKENDLVFLAPERINEEKKDTERNDIQDLHEYHSGYVH
                     KFRRTSVMRNGKTECYLSIQTQENFPANLNELVNCIVISSLVTTQRKLKAMSLLGSRN
                     QLARAVLNPNPMDFCTKDLLTTTSERIIAYLRDFNEDQKKAIETAYAMVKHSPSVAKI
                     CLIHGPPGTGKSKTIVGLLYRLLTENQRKGHSDENSNAKIKQNRVLVCAPSNAAVDEL
                     MKKIILEFKEKCKDKKNPLGNCGDINLVRLGPEKSINSEVLKFSLDSQVNHRMKKELP
                     SHVQAMHKRKEFLDYQLDELSRQRALCRGGREIQRQELDENISKVSKERQELASKIKE
                     VQGRPQKTQSIIILESHIICCTLSTSGGLLLESAFRGQGGVPFSCVIVDEAGQSCEIE
                     TLTPLIHRCNKLILVGDPKQLPPTVISMKAQEYGYDQSMMARFCRLLEENVEHNMISR
                     LPILQLTVQYRMHPDICLFPSNYVYNRNLKTNRQTEAIRCSSDWPFQPYLVFDVGDGS
                     ERRDNDSYINVQEIKLVMEIIKLIKDKRKDVSFRNIGIITHYKAQKTMIQKDLDKEFD
                     RKGPAEVDTVDAFQGRQKDCVIVTCVRANSIQGSIGFLASLQRLNVTITRAKYSLFIL
                     GHLRTLMENQHWNQLIQDAQKRGAIIKTCDKNYRHDAVKILKLKPVLQRSLTHPPTIA
                     PEGSRPQGGLPSSKLDSGFAKTSVAASLYHTPSDSKEITLTVTSKDPERPPVHDQLQD
                     PRLLKRMGIEVKGGIFLWDPQPSSPQHPGATPPTGEPGFPVVHQDLSHIQQPAAVVAA
                     LSSHKPPVRGEPPAASPEASTCQSKCDDPEEELCHRREARAFSEGEQEKCGSETHHTR
                     RNSRWDKRTLEQEDSSSKKRKLL"
     exon            362..572
                     /gene="SETX"
                     /gene_synonym="ALS4; AOA2; bA479K20.2; SCAN2; SCAR1; Sen1;
                     STEX"
                     /inference="alignment:Splign:2.1.0"
     exon            573..682
                     /gene="SETX"
                     /gene_synonym="ALS4; AOA2; bA479K20.2; SCAN2; SCAR1; Sen1;
                     STEX"
                     /inference="alignment:Splign:2.1.0"
     exon            683..902
                     /gene="SETX"
                     /gene_synonym="ALS4; AOA2; bA479K20.2; SCAN2; SCAR1; Sen1;
                     STEX"
                     /inference="alignment:Splign:2.1.0"
     exon            903..1022
                     /gene="SETX"
                     /gene_synonym="ALS4; AOA2; bA479K20.2; SCAN2; SCAR1; Sen1;
                     STEX"
                     /inference="alignment:Splign:2.1.0"
     exon            1023..1194
                     /gene="SETX"
                     /gene_synonym="ALS4; AOA2; bA479K20.2; SCAN2; SCAR1; Sen1;
                     STEX"
                     /inference="alignment:Splign:2.1.0"
     exon            1195..1282
                     /gene="SETX"
                     /gene_synonym="ALS4; AOA2; bA479K20.2; SCAN2; SCAR1; Sen1;
                     STEX"
                     /inference="alignment:Splign:2.1.0"
     exon            1283..5458
                     /gene="SETX"
                     /gene_synonym="ALS4; AOA2; bA479K20.2; SCAN2; SCAR1; Sen1;
                     STEX"
                     /inference="alignment:Splign:2.1.0"
     exon            5459..5558
                     /gene="SETX"
                     /gene_synonym="ALS4; AOA2; bA479K20.2; SCAN2; SCAR1; Sen1;
                     STEX"
                     /inference="alignment:Splign:2.1.0"
     exon            5559..5732
                     /gene="SETX"
                     /gene_synonym="ALS4; AOA2; bA479K20.2; SCAN2; SCAR1; Sen1;
                     STEX"
                     /inference="alignment:Splign:2.1.0"
     exon            5733..5965
                     /gene="SETX"
                     /gene_synonym="ALS4; AOA2; bA479K20.2; SCAN2; SCAR1; Sen1;
                     STEX"
                     /inference="alignment:Splign:2.1.0"
     exon            5966..6133
                     /gene="SETX"
                     /gene_synonym="ALS4; AOA2; bA479K20.2; SCAN2; SCAR1; Sen1;
                     STEX"
                     /inference="alignment:Splign:2.1.0"
     exon            6134..6290
                     /gene="SETX"
                     /gene_synonym="ALS4; AOA2; bA479K20.2; SCAN2; SCAR1; Sen1;
                     STEX"
                     /inference="alignment:Splign:2.1.0"
     exon            6291..6392
                     /gene="SETX"
                     /gene_synonym="ALS4; AOA2; bA479K20.2; SCAN2; SCAR1; Sen1;
                     STEX"
                     /inference="alignment:Splign:2.1.0"
     exon            6393..6508
                     /gene="SETX"
                     /gene_synonym="ALS4; AOA2; bA479K20.2; SCAN2; SCAR1; Sen1;
                     STEX"
                     /inference="alignment:Splign:2.1.0"
     exon            6509..6580
                     /gene="SETX"
                     /gene_synonym="ALS4; AOA2; bA479K20.2; SCAN2; SCAR1; Sen1;
                     STEX"
                     /inference="alignment:Splign:2.1.0"
     exon            6581..6730
                     /gene="SETX"
                     /gene_synonym="ALS4; AOA2; bA479K20.2; SCAN2; SCAR1; Sen1;
                     STEX"
                     /inference="alignment:Splign:2.1.0"
     exon            6731..6838
                     /gene="SETX"
                     /gene_synonym="ALS4; AOA2; bA479K20.2; SCAN2; SCAR1; Sen1;
                     STEX"
                     /inference="alignment:Splign:2.1.0"
     exon            6839..7026
                     /gene="SETX"
                     /gene_synonym="ALS4; AOA2; bA479K20.2; SCAN2; SCAR1; Sen1;
                     STEX"
                     /inference="alignment:Splign:2.1.0"
     exon            7027..7119
                     /gene="SETX"
                     /gene_synonym="ALS4; AOA2; bA479K20.2; SCAN2; SCAR1; Sen1;
                     STEX"
                     /inference="alignment:Splign:2.1.0"
     exon            7120..7284
                     /gene="SETX"
                     /gene_synonym="ALS4; AOA2; bA479K20.2; SCAN2; SCAR1; Sen1;
                     STEX"
                     /inference="alignment:Splign:2.1.0"
     exon            7285..7383
                     /gene="SETX"
                     /gene_synonym="ALS4; AOA2; bA479K20.2; SCAN2; SCAR1; Sen1;
                     STEX"
                     /inference="alignment:Splign:2.1.0"
     exon            7384..7471
                     /gene="SETX"
                     /gene_synonym="ALS4; AOA2; bA479K20.2; SCAN2; SCAR1; Sen1;
                     STEX"
                     /inference="alignment:Splign:2.1.0"
     exon            7472..11101
                     /gene="SETX"
                     /gene_synonym="ALS4; AOA2; bA479K20.2; SCAN2; SCAR1; Sen1;
                     STEX"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8542..8547
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SETX"
                     /gene_synonym="ALS4; AOA2; bA479K20.2; SCAN2; SCAR1; Sen1;
                     STEX"
                     /note="hexamer: AACAAA"
     polyA_site      8564
                     /gene="SETX"
                     /gene_synonym="ALS4; AOA2; bA479K20.2; SCAN2; SCAR1; Sen1;
                     STEX"
                     /note="major polyA site"
     regulatory      10993..10998
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SETX"
                     /gene_synonym="ALS4; AOA2; bA479K20.2; SCAN2; SCAR1; Sen1;
                     STEX"
                     /note="hexamer: AGTAAA"
     polyA_site      11017
                     /gene="SETX"
                     /gene_synonym="ALS4; AOA2; bA479K20.2; SCAN2; SCAR1; Sen1;
                     STEX"
                     /note="major polyA site"
     regulatory      11081..11086
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SETX"
                     /gene_synonym="ALS4; AOA2; bA479K20.2; SCAN2; SCAR1; Sen1;
                     STEX"
                     /note="hexamer: TATAAA"
     polyA_site      11101
                     /gene="SETX"
                     /gene_synonym="ALS4; AOA2; bA479K20.2; SCAN2; SCAR1; Sen1;
                     STEX"
ORIGIN      
        1 acttccgctg gtggcctaga gcggggcccg gtatggaggt gggctagagg ccgacgccag
       61 ccagagagcg aaatgttctt ttggggccag agtctgggca tatatgaatg caaatccgtg
      121 tttgttcaca actaagccca gctgagacga tcacttttct gtaggccatt tgtccaggta
      181 cagaatgagc acatgttgtt ggtgtacgcc aggtggtgct tccaccattg acttcctaaa
      241 gcgctatgct tccaacactc cgtccggtga atttcaaaca gccgacgaag acctctgcta
      301 ctgcttggag tgtgtggctg agtaccacaa agcaagagat gaattgccat tcttgcatga
      361 ggttttatgg gaattagaaa ccttacgtct cataaatcac tttgaaaaat ccatgaaggc
      421 agaaattgga gatgatgatg agttatatat agtagacaat aatggagaga tgccactgtt
      481 tgacatcact gggcaagact ttgaaaataa gcttcgagtt cctcttcttg aaatactgaa
      541 atatccttac ttgcttctac atgaacgtgt taacgagtta tgtgttgaag cactttgtcg
      601 gatggaacaa gccaattgct cctttcaggt gtttgataaa catccaggga tctatttgtt
      661 tttagtccat cccaatgaaa tggttcggcg ttgggctatc ttgactgcaa gaaacttggg
      721 gaaagtggac agagatgatt attatgactt acaagaagtt ttactttgcc tttttaaagt
      781 cattgagttg gggcttttag agagtccaga catttatact tcttctgtcc tagagaaggg
      841 taaactgatt cttctgccct cacacatgta tgatactacc aactacaaaa gctattggtt
      901 aggtatttgc atgttgctga ccattcttga ggaacaagcc atggattccc tgttgttggg
      961 ctcagacaaa caaaatgatt ttatgcaatc gatacttcac actatggaga gggaagcaga
     1021 tgatgatagt gtggatcctt tctggccagc gttacactgt tttatggtga ttctggatcg
     1081 ccttggatct aaggtctggg gtcaacttat ggatcctatt gtggcatttc aaaccattat
     1141 caacaacgca agctacaata gagagatccg acatatacgg aacagctctg taaggaccaa
     1201 gttagaaccg gagtcctatt tggatgatat ggtgacttgc agccagatcg tatacaatta
     1261 taatcctgaa aagaccaaaa aggattctgg atggagaaca gccatttgcc cagattattg
     1321 tcctaacatg tatgaagaaa tggaaacatt agccagtgta cttcagtcag atattggtca
     1381 agacatgcgt gttcataaca gcacatttct atggttcatc ccttttgtcc agtccctcat
     1441 ggatcttaag gatttgggtg tggcttacat agcacaggtt gttaatcatc tgtactctga
     1501 agtcaaagaa gtcctcaacc aaacagatgc tgtgtgtgac aaagtcactg aattttttct
     1561 tctaattttg gtatcagtga ttgaactgca tagaaataaa aaatgtttgc atttgctgtg
     1621 ggtaagttcc cagcaatggg tggaagccgt cgtcaaatgt gccaagcttc ctaccactgc
     1681 gtttacacgg agttctgaga aatcatctgg aaattgctcc aaaggaacag caatgatatc
     1741 ttcactgtca ttgcattcca tgccatctaa ctctgtacaa cttgcttatg tgcagctgat
     1801 tagaagtctc cttaaagaag gttatcagct tgggcagcag tctctttgca agcgattctg
     1861 ggataagctc aacttattcc ttagaggaaa tttatctcta ggttggcagt tgactagtca
     1921 ggaaacccat gagctacaaa gttgcttaaa gcaaattatt agaaacataa aattcaaagc
     1981 acctccatgt aacacttttg tggatctgac ttctgcatgt aaaatctctc ctgcatctta
     2041 taataaagaa gaaagtgaac aaatggggaa gacgtctaga aaagatatgc attgtttgga
     2101 agcttccagc ccaacatttt ctaaagaacc aatgaaagtg caagacagtg tattgatcaa
     2161 agcagataac actatagaag gtgacaataa tgagcaaaat tatataaagg atgtgaaact
     2221 agaggaccat ctcttagctg ggtcatgctt aaagcagagt agtaaaaaca tttttactga
     2281 aagagctgaa gatcaaatta aaataagtac aaggaagcag aagtctgtaa aagagatctc
     2341 ttcatataca ccaaaggact gtacttcaag aaatggtcca gaaaggggat gtgacagagg
     2401 aataatagta tcaacacgtt tgttgactga ttctagcact gatgctttgg aaaaagtgtc
     2461 cacatcgaat gaagatttct ctttaaagga tgatgctctt gctaaaacct caaaacgaaa
     2521 aactaaggta cagaaagatg aaatctgtgc aaagttatca catgtaataa agaagcaaca
     2581 caggaagagt actttggtcg ataatactat caatttagat gaaaatttga ctgtatctaa
     2641 cattgagagt ttctattcaa ggaaagatac aggagttcag aaaggagatg gtttcataca
     2701 caatctttct ttagacccta gtggtgttct ggatgataag aatggagaac aaaaatctca
     2761 aaacaatgta ttgccaaaag agaaacaatt aaagaatgaa gaattagtta ttttctcttt
     2821 ccatgaaaac aattgtaaaa tacaggaatt tcatgttgat ggtaaagaat tgatcccttt
     2881 tacagaaatg accaatgctt cagagaagaa atcatctccc tttaaagatc ttatgactgt
     2941 acctgaatca agagatgagg agatgagtaa tagtaccagt gtgatttatt ctaacttgac
     3001 aagagaacag gcccctgaca tcagtcctaa atctgacacc ttaacggatt ctcagataga
     3061 cagagacctt cacaaattat ctttactagc tcaagccagt gttattacgt tcccatccga
     3121 ttcacctcag aactcatcgc agctgcaaag gaaagtaaaa gaagataaaa gatgtttcac
     3181 agctaaccaa aataatgttg gagatacctc ccgtggacag gttattatta tttcagattc
     3241 tgatgatgat gatgatgaaa gaatcctgag tcttgagaaa ctcactaaac aggacaaaat
     3301 atgccttgag agggaacatc cagagcagca cgtttcaaca gttaatagta aggaggaaaa
     3361 gaatccagta aaggaagaaa agacagagac tctttttcag tttgaggaat ctgattctca
     3421 gtgttttgag tttgaaagtt catctgaagt gttttcagtt tggcaagatc atccagacga
     3481 taataattca gttcaagatg gtgagaaaaa atgtttggct cctatagcca atactacaaa
     3541 tggtcagggt tgtacagatt atgtatctga agttgttaaa aaaggagcag agggcattga
     3601 agaacacaca agaccacgga gtatttctgt tgaagaattt tgtgaaattg aagtaaaaaa
     3661 gcctaagaga aaacgatctg aaaaaccaat ggctgaagat cctgtgaggc cttcatcttc
     3721 tgtcagaaat gagggccagt ctgatactaa taagagagat cttgtgggaa atgattttaa
     3781 aagtattgat agaaggactt caactcccaa ttcacgtatt cagagagcca ctacggtttc
     3841 acaaaagaag tcttcaaagc tttgtacttg tacagaaccc atcaggaaag ttccagtttc
     3901 taagacccct aagaaaactc attcagatgc caaaaaagga cagaatagaa gttcaaatta
     3961 cctaagttgt agaacaactc ctgctatagt gccgccaaag aaatttcgtc agtgtcctga
     4021 gccaacttca acagctgaga aacttggcct gaaaaagggt cctcgtaagg catatgagtt
     4081 gtcccagcgg tctttggatt atgtagctca attacgtgat catggcaaaa ctgttggagt
     4141 agttgatacc cgaaaaaaga ctaaattaat ttctcctcag aacctgtctg tcagaaataa
     4201 taagaaactt ctgactagtc aagaacttca gatgcaaagg cagatcagac ccaaatcaca
     4261 aaaaaataga cgaagacttt ctgattgtga aagtacagat gttaaaagag cagggtcaca
     4321 tacagcacag aattctgaca tatttgtacc agaatctgat aggtcagatt ataattgtac
     4381 aggaggaact gaggtacttg ccaacagtaa cagaaaacag ttaataaaat gcatgccttc
     4441 tgaaccagaa accataaaag caaaacatgg gtctccagca actgatgatg cttgcccttt
     4501 gaaccagtgt gattctgtag tgttaaatgg aacagtacca acaaatgaag taattgtctc
     4561 cacttcagaa gaccctctgg gtggaggtga tccaacagca cgtcatatag agatggcagc
     4621 tttgaaagaa ggagagcctg actccagcag tgatgcagag gaagataact tatttttaac
     4681 ccaaaatgat cctgaagata tggatttatg ttcacaaatg gagaatgaca attataaact
     4741 cattgaacta attcatggaa aagatacagt tgaggttgaa gaagattctg taagtcggcc
     4801 tcagttggaa tctttgagtg gcacaaagtg taagtacaaa gattgtcttg aaaccacaaa
     4861 aaaccagggt gaatactgcc caaaacactc tgaagtgaaa gcagcagatg aagatgtatt
     4921 tcgtaaacct ggcttgcctc ctcctgcatc taaacctttg agacctacca ctaagatttt
     4981 tagctcaaag agtacttcac gaattgctgg tctttctaaa tctttggaaa cttcttcagc
     5041 actttcaccg tctctaaaaa ataagtcaaa ggggatacag tcgattttga aagtaccaca
     5101 gccagttccc ctcatagctc agaagccagt tggtgaaatg aagaattcgt gcaatgttct
     5161 tcatcctcag tctccgaata attccaacag gcaaggttgc aaagttccat ttggtgaaag
     5221 caaatatttt ccatcttcct ctccagtaaa cattcttttg tcatcacagt ctgtctctga
     5281 caccttcgtt aaagaggtct taaaatggaa atatgaaatg tttttgaact ttggtcagtg
     5341 tgggccccct gcaagtcttt gtcagtccat ctcaagacct gtgcctgtca gatttcacaa
     5401 ttatggagat tattttaatg tttttttccc tttgatggta ttgaatactt ttgaaacagt
     5461 ggcacaagaa tggctcaact ctccaaatag agagaatttc tatcagttgc aagtacgaaa
     5521 atttcctgcc gattatataa aatactggga gtttgcagtt tatctggaag aatgtgaact
     5581 ggctaaacag ctttatccaa aggaaaacga tttggtgttt ttagctcctg agagaataaa
     5641 tgaagagaag aaagatacag agagaaatga catacaagat ctccacgaat atcattctgg
     5701 ttatgttcat aaatttcgcc gcacgtcagt catgcgtaat gggaaaactg agtgttacct
     5761 ttccatccag actcaagaga actttccggc caatttaaac gaacttgtga attgtattgt
     5821 aatcagttct ctggtaacta cacaaaggaa gttgaaagcc atgtctctgt tgggtagtcg
     5881 gaaccaactg gctagagctg ttctgaatcc aaaccctatg gacttctgta caaaagattt
     5941 actgactaca acatctgaga gaattattgc gtacttaaga gatttcaatg aagatcaaaa
     6001 gaaagcaata gaaactgcat atgctatggt gaaacactca ccatcagttg ccaaaatctg
     6061 cttgattcat ggaccacctg gaacaggaaa atcaaaaact attgttggcc tcctctatcg
     6121 tctactgaca gagaaccaga ggaaggggca ttcagacgaa aactccaatg ccaaaatcaa
     6181 acaaaaccgt gtcctcgtgt gtgcaccttc caatgcagct gttgatgaac tcatgaaaaa
     6241 aattatcctt gaattcaaag aaaaatgtaa agacaagaag aatcctttag gaaactgtgg
     6301 agatataaat ttagtacgac tgggtccaga aaagtctatt aatagtgagg ttctaaagtt
     6361 cagtttggac agccaagtaa accacagaat gaaaaaagag ttaccttctc atgttcaggc
     6421 gatgcataaa agaaaggaat ttctagatta tcagctggat gagctttccc ggcagcgagc
     6481 tctatgccga ggtggacggg aaatacagag gcaagaatta gatgaaaaca tttccaaagt
     6541 ttctaaggaa aggcaggaac ttgcttctaa aattaaagag gttcaaggac gcccacagaa
     6601 aacacagagt atcatcatct tagagtccca tatcatctgc tgcacgttga gcacaagtgg
     6661 tggtttacta cttgagtctg ctttccgtgg gcaagggggt gtccccttca gctgtgtcat
     6721 tgttgatgag gctggacagt cttgtgaaat tgagactctt actccactca tccatcgctg
     6781 caataagctc atcctagtag gagatcctaa gcagctccct ccgacagtca tctctatgaa
     6841 agcacaggag tatggctacg accagtcaat gatggctcgc ttctgcagac tgctggaaga
     6901 gaatgtagaa cacaacatga tcagcaggct gcccattcta cagctcactg ttcagtacag
     6961 gatgcatcca gacatatgcc tcttcccttc taattatgtt tataacagaa acttaaaaac
     7021 aaatagacag acagaagcca ttcgatgttc atcagattgg ccatttcagc cataccttgt
     7081 gtttgatgtt ggagatggtt cagaaagacg ggataatgac tcatatataa atgttcaaga
     7141 aataaaactg gtgatggaaa taattaagct tattaaagac aaaagaaagg atgttagttt
     7201 tcgaaacatt ggcataataa ctcattacaa ggcccagaag acgatgattc agaaggattt
     7261 ggacaaagag ttcgatagaa aaggaccagc agaagtagac actgtggatg cattccaggg
     7321 tcggcagaag gattgtgtta ttgttacgtg tgtcagagca aatagcatcc aaggttcaat
     7381 tggattcctg gcaagtttgc agagattgaa tgtcaccatc acacgagcca agtacagcct
     7441 cttcatcctc ggacatttga ggaccctgat ggaaaaccag cattggaatc agctgattca
     7501 ggatgctcag aagcgtggtg ccattattaa gacctgtgac aaaaactata gacatgatgc
     7561 agtgaagatt ctgaaactca agcctgtgct gcagagaagt ctcactcacc ctcctaccat
     7621 agccccagag gggtccagac cccagggtgg tttgcccagc agcaagctag acagtggatt
     7681 tgccaagaca tctgttgctg cttctctata ccacacaccc tctgactcca aggaaattac
     7741 tcttactgtt acttcaaagg accctgaaag acctcctgtt catgaccaac ttcaggaccc
     7801 acgactgctg aagaggatgg gcattgaggt caaaggagga atattccttt gggatccaca
     7861 accctcgagc ccccagcatc ctggagcaac acctcctacg ggcgagccgg gcttccctgt
     7921 cgttcaccag gacctgagcc atatacagca gcccgctgct gtagtggctg ctctgagcag
     7981 ccacaaacct cccgtgcggg gcgaacctcc agctgccagt cccgaggctt ccacgtgtca
     8041 gagcaaatgt gatgacccgg aagaggagct ctgtcacagg agagaggcca gggctttcag
     8101 tgaaggggag caggagaagt gtggttccga gacccatcac accaggagga actctaggtg
     8161 ggacaagagg acactggagc aggaggacag cagttccaag aaaagaaagc ttttatagga
     8221 aagcccagtg acatgggcca gcagccacag catattgtaa actgaagatg accagctcgt
     8281 gggaccatct agataagctt gttttttgta aggagtttgt gtgctgttgg aaaacatgga
     8341 aaatgcatcc ttaacacctg agcctctggt catcttcagt attttctgtc atttgcaaaa
     8401 gctttcagag ggcattgtgt atccgtaata atgtccttga agtcagagac tggaaatgtt
     8461 gatctcttag tcttctatag acaaggaggt cagactggag tgaatgttgt aaaagttgaa
     8521 atgtatattt gtatagcaaa taacaaaatc cttttaaaat ttaatctagt agaccgcttt
     8581 tctttccccc tgtttaaaat gttaatcagt tttcaacaga acaaacttta tattaccaaa
     8641 aaaaaaaaga tgggaggagg gcgggtcctt tcaaccattg ttagagcaag acagataatt
     8701 atttaacagc ctagcttgga ataagctgag ttagtgctgg tgggtcaggt gtctctggct
     8761 ctattaaaaa aagcaaaaac ccccccaaaa acctggagtc tcctagggga cactttgggc
     8821 agcacggtta tgttaggtaa agtcttgctg acatggtgca tttttagata gagtgcattg
     8881 gcccagggta ttatatttct gtgatgagtt catttacctg tttcagtatg cacatagttc
     8941 cctagctaaa attcctaatc ttcttgagag agaagaatat ggagtgaaag aataattctt
     9001 gagctatcat tcaaatgctc ccagcatatt gtgagccttg tgtgactggt gaggtacaga
     9061 catttggttg ccaaatgcta gattagcgct gggtcagctg tggaagaatc gcctcagctt
     9121 acagattgtc agacagatct aactagtttt ccagaaagcc tgggaagctg tgtgttcaac
     9181 atttcccaag ggattctgat caccaggcag cttgggaacc actggggcag gccaaataga
     9241 atattttggg cgggaaagaa gcacccgatt taaaatgaag cgtaaccaga ggagttcaga
     9301 actgggaaga gagtggtaga cttcctgtga tcttcagaaa tcatctacct ggtaaaaata
     9361 catgctgttt agaatatctg ataggtgttt ccagctacta ttagaggtga tagtgctttt
     9421 gtgggggaaa aaattggtca tggtgaatgg agatcgagga agctcgggac aagggagggg
     9481 tgggctgcct gattttgtcc agttttccaa atatccacgc agtgaactgg agtatcctaa
     9541 acatgagaat gtacagttga cagttgtaaa aactagggat ctgtagtgaa tgctgtgcag
     9601 ccccatatct catttggggg taggaaaata gctgaagatt catgtgcatt atttgacatt
     9661 tcctttgtca tctgcttttt aagcaaaaaa gggttttgtg ttagaaattc tacttgagca
     9721 gattataaag agctttaaaa aacaactttc ggttgccaaa agtttgagca tttgatttca
     9781 ttacctgtgt ctccctcact ggtgtccaga cggtcaactg aatactcctg aaacccaggg
     9841 agcaggtgac ttcctggagt gctttgtccc cagagtcagc cactgcttcc tctgtggggg
     9901 tggagagttt gtctttggcc atgcagtgtg cgacagttca ggacgggtag ggatgggtcc
     9961 cattctgtct gggtcaaggg ctctatcagc ttcttccatg tgcctttggg aagaaatctc
    10021 gttactttaa gtttgctttc ctgttatctt gatgaagtgc ccattttagc agacacttgt
    10081 agtgctgacc acttagggaa tgtacaaact cctaagcttc taaagggagg catggcaaaa
    10141 acgttggggt caggatgtct ctcacgctgc tcatgttaat actattaaca catgatttga
    10201 gaaataagtt ttctctaaaa tgcatatttt gccgccacac actgaacaat attatttcca
    10261 gtgaagtttg atgcctgttc ttacgttgtg ttcacctgtt ggttcaccac tcagcagatc
    10321 tgattctgca agaattaatg gtagaactag atcatccttt ctaacagacg agcctgtgtc
    10381 ctgtgacggc ctttcacagc ggaatgcagt tgtacctcac attacttttg aaacttcact
    10441 cgttccagtt ggtacaagta tttgccaaag ccatttccta tgttcaccgt ggcccctcct
    10501 gatgtggctg tcagcgcagc gttgttgaac agggctattc tttttacaag gtgtgaagtg
    10561 tggctcttcg cttcgtcttt gccatggcat taaaagaaag ttccctgtct tctttcaata
    10621 ttagttattt caaatgaata tgtgctactt aaaagcttgt tttgtttctt tgtatataat
    10681 ttgccttgga tttattgtgc acagtttgtt gagttgtatg tttttgtgaa ttatcaggag
    10741 taaatttgac aagtacatgt gaataacctc ctgtaaatga attttataac aaaaatgtac
    10801 tgaactattt tttaaagttg tgcagattag caattttttg ctatagcttt gacttttcta
    10861 tgctgtgaat taatagctgc gatttggcaa acagccctgt tgtctttgtt aaaccctaaa
    10921 ttttaagagg aaatggcaga attaaaagca gaaacaagaa gatggacatg gattagaggt
    10981 tatgtattat gaagtaaact acaaggtact aacatcattt cgtctgccat ttggtttgct
    11041 ttatgctgaa attacttggt ggggatttgt gcaattcaga tataaaaagt ttcattatcc
    11101 a
//
