LOCUS       NM_001160308            6203 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens SET domain bifurcated histone lysine methyltransferase
            2 (SETDB2), transcript variant 2, mRNA.
ACCESSION   NM_001160308 XM_005266568
VERSION     NM_001160308.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6203)
  AUTHORS   Mangum,K.D., denDekker,A., Li,Q., Tsoi,L.C., Joshi,A.D.,
            Melvin,W.J., Wolf,S.J., Moon,J.Y., Audu,C.O., Shadiow,J., Obi,A.T.,
            Wasikowski,R., Barrett,E.C., Bauer,T.M., Boyer,K., Ahmed,Z.,
            Davis,F.M., Gudjonsson,J. and Gallagher,K.A.
  TITLE     The STAT3/SETDB2 axis dictates NF-kappaB-mediated inflammation in
            macrophages during wound repair
  JOURNAL   JCI Insight 9 (20), e179017 (2024)
   PUBMED   39435663
  REMARK    GeneRIF: The STAT3/SETDB2 axis dictates NF-kappaB-mediated
            inflammation in macrophages during wound repair.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6203)
  AUTHORS   Mahana,Y., Ariyoshi,M., Nozawa,R.S., Shibata,S., Nagao,K., Obuse,C.
            and Shirakawa,M.
  TITLE     Structural evidence for protein-protein interaction between the
            non-canonical methyl-CpG-binding domain of SETDB proteins and
            C11orf46
  JOURNAL   Structure 32 (3), 304-315 (2024)
   PUBMED   38159574
  REMARK    GeneRIF: Structural evidence for protein-protein interaction
            between the non-canonical methyl-CpG-binding domain of SETDB
            proteins and C11orf46.
REFERENCE   3  (bases 1 to 6203)
  AUTHORS   Sonobe,S., Kitabatake,M., Hara,A., Konda,M., Ouji-Sageshima,N.,
            Terada-Ikeda,C., Furukawa,R., Imakita,N., Oda,A., Takeda,M.,
            Takamura,S., Inoue,S., Kunkel,S.L., Kawaguchi,M. and Ito,T.
  TITLE     THE CRITICAL ROLE OF THE HISTONE MODIFICATION ENZYME SETDB2 IN THE
            PATHOGENESIS OF ACUTE RESPIRATORY DISTRESS SYNDROME
  JOURNAL   Shock 60 (1), 137-145 (2023)
   PUBMED   37195726
  REMARK    GeneRIF: THE CRITICAL ROLE OF THE HISTONE MODIFICATION ENZYME
            SETDB2 IN THE PATHOGENESIS OF ACUTE RESPIRATORY DISTRESS SYNDROME.
REFERENCE   4  (bases 1 to 6203)
  AUTHORS   Yuan,G., Hu,B., Ma,J., Zhang,C., Xie,H., Wei,T., Yang,Y. and Ni,B.
  TITLE     Histone lysine methyltransferase SETDB2 suppresses NRF2 to restrict
            tumor progression and modulates chemotherapy sensitivity in lung
            adenocarcinoma
  JOURNAL   Cancer Med 12 (6), 7258-7272 (2023)
   PUBMED   36504353
  REMARK    GeneRIF: Histone lysine methyltransferase SETDB2 suppresses NRF2 to
            restrict tumor progression and modulates chemotherapy sensitivity
            in lung adenocarcinoma.
REFERENCE   5  (bases 1 to 6203)
  AUTHORS   Melvin,W.J., Audu,C.O., Davis,F.M., Sharma,S.B., Joshi,A.,
            DenDekker,A., Wolf,S., Barrett,E., Mangum,K., Zhou,X., Bame,M.,
            Ruan,A., Obi,A., Kunkel,S.L., Moore,B.B. and Gallagher,K.A.
  TITLE     Coronavirus induces diabetic macrophage-mediated inflammation via
            SETDB2
  JOURNAL   Proc Natl Acad Sci U S A 118 (38) (2021)
   PUBMED   34479991
  REMARK    GeneRIF: Coronavirus induces diabetic macrophage-mediated
            inflammation via SETDB2.
REFERENCE   6  (bases 1 to 6203)
  AUTHORS   Ocklenburg,S., Arning,L., Gerding,W.M., Hengstler,J.G.,
            Epplen,J.T., Gunturkun,O., Beste,C. and Akkad,D.A.
  TITLE     Left-Right Axis Differentiation and Functional Lateralization: a
            Haplotype in the Methyltransferase Encoding Gene SETDB2 Might
            Mediate Handedness in Healthy Adults
  JOURNAL   Mol Neurobiol 53 (9), 6355-6361 (2016)
   PUBMED   26572639
  REMARK    GeneRIF: the relation of genetic variation in SETDB2-and its
            paralogue SETDB1-with different handedness phenotypes in 950
            healthy adult participants, was investigated.
REFERENCE   7  (bases 1 to 6203)
  AUTHORS   Holt,R.J., Vandiedonck,C., Willis-Owen,S.A., Knight,J.C.,
            Cookson,W.O., Moffatt,M.F. and Zhang,Y.
  TITLE     A functional AT/G polymorphism in the 5'-untranslated region of
            SETDB2 in the IgE locus on human chromosome 13q14
  JOURNAL   Genes Immun 16 (7), 488-494 (2015)
   PUBMED   26378653
  REMARK    GeneRIF: Results indicate that the IgE-associated AT/G polymorphism
            (rs386770867) regulates transcription of SETDB2.
            Erratum:[Genes Immun. 2017 Jan;18(1):57. doi:
            10.1038/gene.2016.22.. PMID: 27170559]
REFERENCE   8  (bases 1 to 6203)
  AUTHORS   Falandry,C., Fourel,G., Galy,V., Ristriani,T., Horard,B.,
            Bensimon,E., Salles,G., Gilson,E. and Magdinier,F.
  TITLE     CLLD8/KMT1F is a lysine methyltransferase that is important for
            chromosome segregation
  JOURNAL   J Biol Chem 285 (26), 20234-20241 (2010)
   PUBMED   20404330
  REMARK    GeneRIF: Results provide evidence that CLLD8/KMT1F is recruited to
            heterochromatin regions and contributes in vivo to the deposition
            of trimethyl marks in concert with SUV39H1/KMT1A.SUV39H1/KMT1A.
REFERENCE   9  (bases 1 to 6203)
  AUTHORS   Zhang,Y., Leaves,N.I., Anderson,G.G., Ponting,C.P., Broxholme,J.,
            Holt,R., Edser,P., Bhattacharyya,S., Dunham,A., Adcock,I.M.,
            Pulleyn,L., Barnes,P.J., Harper,J.I., Abecasis,G., Cardon,L.,
            White,M., Burton,J., Matthews,L., Mott,R., Ross,M., Cox,R.,
            Moffatt,M.F. and Cookson,W.O.
  TITLE     Positional cloning of a quantitative trait locus on chromosome
            13q14 that influences immunoglobulin E levels and asthma
  JOURNAL   Nat Genet 34 (2), 181-186 (2003)
   PUBMED   12754510
REFERENCE   10 (bases 1 to 6203)
  AUTHORS   Mabuchi,H., Fujii,H., Calin,G., Alder,H., Negrini,M., Rassenti,L.,
            Kipps,T.J., Bullrich,F. and Croce,C.M.
  TITLE     Cloning and characterization of CLLD6, CLLD7, and CLLD8, novel
            candidate genes for leukemogenesis at chromosome 13q14, a region
            commonly deleted in B-cell chronic lymphocytic leukemia
  JOURNAL   Cancer Res 61 (7), 2870-2877 (2001)
   PUBMED   11306461
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL136218.26, DB164537.1,
            BC047434.2, BC028202.1, AL139321.28 and AW297242.1.
            
            On Jun 2, 2019 this sequence version replaced NM_001160308.2.
            
            Summary: This gene encodes a member of a family of proteins that
            contain a methyl-CpG-binding domain (MBD) and a SET domain and
            function as histone methyltransferases. This protein is recruited
            to heterochromatin and plays a role in the regulation of chromosome
            segregation. This region is commonly deleted in chronic lymphocytic
            leukemia. Naturally-occuring readthrough transcription occurs from
            this gene to the downstream PHF11 (PHD finger protein 11) gene.
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Mar 2016].
            
            Transcript Variant: This variant (2) lacks an alternate in-frame
            exon compared to variant 1. The encoded isoform (b) is shorter than
            isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803617.201209.1,
                                           SRR1660807.148088.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000611815.2/ ENSP00000482240.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-19                AL136218.26        115393-115411
            20-584              DB164537.1         1-565
            585-1238            BC047434.2         2-655
            1239-2501           BC028202.1         693-1955
            2502-3322           BC047434.2         1919-2739
            3323-5874           AL139321.28        3479-6030
            5875-6203           AW297242.1         17-345              c
FEATURES             Location/Qualifiers
     source          1..6203
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="13"
                     /map="13q14.2"
     gene            1..6203
                     /gene="SETDB2"
                     /gene_synonym="C13orf4; CLLD8; CLLL8; KMT1F"
                     /note="SET domain bifurcated histone lysine
                     methyltransferase 2"
                     /db_xref="GeneID:83852"
                     /db_xref="HGNC:HGNC:20263"
                     /db_xref="MIM:607865"
     exon            1..584
                     /gene="SETDB2"
                     /gene_synonym="C13orf4; CLLD8; CLLL8; KMT1F"
                     /inference="alignment:Splign:2.1.0"
     exon            585..941
                     /gene="SETDB2"
                     /gene_synonym="C13orf4; CLLD8; CLLL8; KMT1F"
                     /inference="alignment:Splign:2.1.0"
     CDS             926..3049
                     /gene="SETDB2"
                     /gene_synonym="C13orf4; CLLD8; CLLL8; KMT1F"
                     /EC_number="2.1.1.366"
                     /note="isoform b is encoded by transcript variant 2;
                     lysine N-methyltransferase 1F; histone-lysine
                     N-methyltransferase SETDB2; chronic lymphocytic leukemia
                     deletion region gene 8 protein; SET domain bifurcated 2"
                     /codon_start=1
                     /product="histone-lysine N-methyltransferase SETDB2
                     isoform b"
                     /protein_id="NP_001153780.1"
                     /db_xref="CCDS:CCDS53868.1"
                     /db_xref="GeneID:83852"
                     /db_xref="HGNC:HGNC:20263"
                     /db_xref="MIM:607865"
                     /translation="MGEKNGDAKTFWMELEDDGKVDFIFEQVQNVLQSLKQKIKDGSA
                     TNKEYIQAMILVNEATIINSSTSIKDPMPVTQKEQENKSNAFPSTSCENSFPEDCTFL
                     TTENKEILSLEDKVVDFREKDSSSNLSYQSHDCSGACLMKMPLNLKGENPLQLPIKCH
                     FQRRHAKTNSHSSALHVSYKTPCGRSLRNVEEVFRYLLETECNFLFTDNFSFNTYVQL
                     ARNYPKQKEVVSDVDISNGVESVPISFCNEIDSRKLPQFKYRKTVWPRAYNLTNFSSM
                     FTDSCDCSEGCIDITKCACLQLTARNAKTSPLSSDKITTGYKYKRLQRQIPTGIYECS
                     LLCKCNRQLCQNRVVQHGPQVRLQVFKTEQKGWGVRCLDDIDRGTFVCIYSGRLLSRA
                     NTEKSYGIDENGRDENTMKNIFSKKRKLEVACSDCEVEVLPLGLETHPRTAKTEKCPP
                     KFSNNPKELTVETKYDNISRIQYHSVIRDPESKTAIFQHNGKKMEFVSSESVTPEDND
                     GFKPPREHLNSKTKGAQKDSSSNHVDEFEDNLLIESDVIDITKYREETPPRSRCNQAT
                     TLDNQNIKKAIEVQIQKPQEGRSTACQRQQVFCDEELLSETKNTSSDSLTKFNKGNVF
                     LLDATKEGNVGRFLNHSCCPNLLVQNVFVETHNRNFPLVAFFTNRYVKARTELTWDYG
                     YEAGTVPEKEIFCQCGVNKCRKKIL"
     exon            942..1067
                     /gene="SETDB2"
                     /gene_synonym="C13orf4; CLLD8; CLLL8; KMT1F"
                     /inference="alignment:Splign:2.1.0"
     exon            1068..1133
                     /gene="SETDB2"
                     /gene_synonym="C13orf4; CLLD8; CLLL8; KMT1F"
                     /inference="alignment:Splign:2.1.0"
     exon            1134..1230
                     /gene="SETDB2"
                     /gene_synonym="C13orf4; CLLD8; CLLL8; KMT1F"
                     /inference="alignment:Splign:2.1.0"
     exon            1231..1794
                     /gene="SETDB2"
                     /gene_synonym="C13orf4; CLLD8; CLLL8; KMT1F"
                     /inference="alignment:Splign:2.1.0"
     exon            1795..1911
                     /gene="SETDB2"
                     /gene_synonym="C13orf4; CLLD8; CLLL8; KMT1F"
                     /inference="alignment:Splign:2.1.0"
     exon            1912..2081
                     /gene="SETDB2"
                     /gene_synonym="C13orf4; CLLD8; CLLL8; KMT1F"
                     /inference="alignment:Splign:2.1.0"
     exon            2082..2307
                     /gene="SETDB2"
                     /gene_synonym="C13orf4; CLLD8; CLLL8; KMT1F"
                     /inference="alignment:Splign:2.1.0"
     exon            2308..2407
                     /gene="SETDB2"
                     /gene_synonym="C13orf4; CLLD8; CLLL8; KMT1F"
                     /inference="alignment:Splign:2.1.0"
     exon            2408..2501
                     /gene="SETDB2"
                     /gene_synonym="C13orf4; CLLD8; CLLL8; KMT1F"
                     /inference="alignment:Splign:2.1.0"
     exon            2502..2842
                     /gene="SETDB2"
                     /gene_synonym="C13orf4; CLLD8; CLLL8; KMT1F"
                     /inference="alignment:Splign:2.1.0"
     exon            2843..2931
                     /gene="SETDB2"
                     /gene_synonym="C13orf4; CLLD8; CLLL8; KMT1F"
                     /inference="alignment:Splign:2.1.0"
     exon            2932..6203
                     /gene="SETDB2"
                     /gene_synonym="C13orf4; CLLD8; CLLL8; KMT1F"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3095..3100
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SETDB2"
                     /gene_synonym="C13orf4; CLLD8; CLLL8; KMT1F"
                     /note="hexamer: ATTAAA"
     polyA_site      3135
                     /gene="SETDB2"
                     /gene_synonym="C13orf4; CLLD8; CLLL8; KMT1F"
                     /note="major polyA site"
     regulatory      6177..6182
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SETDB2"
                     /gene_synonym="C13orf4; CLLD8; CLLL8; KMT1F"
                     /note="hexamer: AATAAA"
     polyA_site      6203
                     /gene="SETDB2"
                     /gene_synonym="C13orf4; CLLD8; CLLL8; KMT1F"
                     /note="major polyA site"
ORIGIN      
        1 ggaggttacg ccttccctca tccccggtag aggcagggcg ggactgttgt ggttgagatg
       61 aaggctagta aatggtgaag tacttcccgg ccagagggca cctgcgctcg ggaggtttgg
      121 gcggcttggc gtcggaggag agccccaccc gcggaggaac ccagccttgc caacggagct
      181 ggcggagctc actcctcagg tcaggcgggc ggcgtagaaa acgcagcgga gccaggtgaa
      241 accaaggcac cgccgtggct ggcccccgac agttcctcta gccgggaggt tggaggagct
      301 gaaaacgccg cggagccctc ggccgcccga gcaggggctg gaccccagcc cttgcagcct
      361 cccttctcct ggcacccaag tgcagtcctg gctgcagaag gggccgcggg cgcactgagt
      421 ttccaacctc catttcagcc tgtctgtctc agggtgcagc cttaatgaga ggtgattcct
      481 aagctgctgg gaacctgagg ttgtcaaagg ggcggcagga aatggacagc agtataaaac
      541 ccagaagcag aacttgaagg ttaaaccact agcccatttc acagaatgtt tcatccattt
      601 gtggaccaaa agatggagtt ggtttttatt tttaaaaaga taatgttaat gatctgatac
      661 cactacaaat atttacgtga gaagattcat ggacttgtct tttggttgga ctgtcactca
      721 tttctgaaag tttcttcagc cacaatttct atttgaaaat tcaagtatca aaggatacca
      781 ggtttagaat ggtataatga tgtattttgt ctgaggactg caaattttat agagaccaca
      841 gttggattcc agtgatattc tgcaatcaaa gtgatttgat aaacctaatt ttgaagcatt
      901 ttatatttat aagcgacatc aaaagatggg agaaaaaaat ggcgatgcaa aaactttctg
      961 gatggagcta gaagatgatg gaaaagtgga cttcattttt gaacaagtac aaaatgtgct
     1021 gcagtcactg aaacaaaaga tcaaagatgg gtctgccacc aataaagaat acatccaagc
     1081 aatgattcta gtgaatgaag caactataat taacagttca acatcaataa aggatcctat
     1141 gcctgtgact cagaaggaac aggaaaacaa atccaatgca tttccctcta catcatgtga
     1201 aaactccttt ccagaagact gtacatttct aacaacagaa aataaggaaa ttctctctct
     1261 tgaagataaa gttgtagact ttagagaaaa agactcatct tcgaatttat cttaccaaag
     1321 tcatgactgc tctggtgctt gtctgatgaa aatgccactg aacttgaagg gagaaaaccc
     1381 tctgcagctg ccaatcaaat gtcacttcca aagacgacat gcaaagacaa actctcattc
     1441 ttcagcactc cacgtgagtt ataaaacccc ttgtggaagg agtctacgaa acgtggagga
     1501 agtttttcgt tacctgcttg agacagagtg taacttttta tttacagata acttttcttt
     1561 caatacctat gttcagttgg ctcggaatta cccaaagcaa aaagaagttg tttctgatgt
     1621 ggatattagc aatggagtgg aatcagtgcc catttctttc tgtaatgaaa ttgacagtag
     1681 aaagctccca cagtttaagt acagaaagac tgtgtggcct cgagcatata atctaaccaa
     1741 cttttccagc atgtttactg attcctgtga ctgctctgag ggctgcatag acataacaaa
     1801 atgtgcatgt cttcaactga cagcaaggaa tgccaaaact tcccccttgt caagtgacaa
     1861 aataaccact ggatataaat ataaaagact acagagacag attcctactg gcatttatga
     1921 atgcagcctt ttgtgcaaat gtaatcgaca attgtgtcaa aaccgagttg tccaacatgg
     1981 tcctcaagtg aggttacagg tgttcaaaac tgagcagaag ggatggggtg tacgctgtct
     2041 agatgacatt gacagaggga catttgtttg catttattca ggaagattac taagcagagc
     2101 taacactgaa aaatcttatg gtattgatga aaacgggaga gatgagaata ctatgaaaaa
     2161 tatattttca aaaaagagga aattagaagt tgcatgttca gattgtgaag ttgaagttct
     2221 cccattagga ttggaaacac atcctagaac tgctaaaact gagaaatgtc caccaaagtt
     2281 cagtaataat cccaaggagc ttactgtgga aacgaaatat gataatattt caagaattca
     2341 atatcattca gttattagag atcctgaatc caagacagcc atttttcaac acaatgggaa
     2401 aaaaatggaa tttgtttcct cggagtctgt cactccagaa gataatgatg gatttaaacc
     2461 accccgagag catctgaact ctaaaaccaa gggagcacaa aaggactcaa gttcaaacca
     2521 tgttgatgag tttgaagata atctgctgat tgaatcagat gtgatagata taactaaata
     2581 tagagaagaa actccaccaa ggagcagatg taaccaggcg accacattgg ataatcagaa
     2641 tattaaaaag gcaattgagg ttcaaattca gaaaccccaa gagggacgat ctacagcatg
     2701 tcaaagacag caggtatttt gtgatgaaga gttgctaagt gaaaccaaga atacttcatc
     2761 tgattctcta acaaagttca ataaagggaa tgtgttttta ttggatgcca caaaagaagg
     2821 aaatgtcggc cgcttcctta atcatagttg ttgcccaaat ctcttggtac agaatgtttt
     2881 tgtagaaaca cacaacagga attttccatt ggtggcattc ttcaccaaca ggtatgtgaa
     2941 agcaagaaca gagctaacat gggattatgg ctatgaagct gggactgtgc ctgagaagga
     3001 aatcttctgc caatgtgggg ttaataaatg tagaaaaaaa atattataaa tatgtaacta
     3061 acgcctgttt gtgaaattag cttatcaggc tgaaattaaa gccatgcaaa agaaggtcta
     3121 ggtccatcaa ggaaattccc ctccgttttc ctttgtcatg gggtttatgt tttatttcag
     3181 attttatttg tgtgacttag aaattccagg aacacaatta ggatattttc atacacatag
     3241 ggtatcttgt tcactgctgt gctactttac atgagtagga tggaagtgta tattttatat
     3301 gaaataccac tgtacaattt ataatttatt tacaaattat atattaagag aaacaaatgt
     3361 cataacagaa ctcagctgtt tctaattgct tttgtgactg ttacctttta gttcatgccc
     3421 ccccaaagag ctaaatttca catttttacc tacaaaattg atttttaatt cctggcaaat
     3481 aatttaccat tatgagctac aaggtgggca acagcgcctg aggatctaat tttatgcata
     3541 ttactcccaa gtattttaac acttgttgga gaagcaatat ctggatcgat aaaacactgt
     3601 cccatcaacc atttgagtgg ggagagggag aagctcttct gtaagtaaga ttctggcaag
     3661 ctctttgaaa tgagtcttct ttcccacaga ttttctctac tctttctata caaacagata
     3721 ggagaagagg gaatagaaac ctggaggaac ttgaatattt ttgttctaga tagagataca
     3781 gttactgaaa aggaaaccta gaaagtagtc acacgttgct tatttaggcc agaagtaatt
     3841 gtactgggca aaaatttcac ttaaaaaaca caagaagtcc aggtatggtg gctcagacct
     3901 gtaatcccag cactttgaga ggccgaggca ggtggattac ttgagcctag gggttcaaga
     3961 ccagcttggg caacatgtca aaaccctgtc tctacaaaaa atacaaaaat tagcctggca
     4021 tgatggcatg tgcccgtagt ctcagctact caggagtgag gtgggaggat catttgagct
     4081 cagaaggtca aggctgcaat gagacataat ttcaccatag tacttccagc ctgggcaata
     4141 gagcaagact ctctctcaaa aaaaacagca cacacacaca cacacgaaaa caattctgaa
     4201 ctatgaaatc tgaaacagcc ccttggtatc tcctgggcat gatttgcaaa tctttttttt
     4261 ttacagaaaa aaggcaaaga gtaagcactt tgccataggt tacttggccg tgatcatcta
     4321 tctagtggaa aaggggactg ggaagcccaa gcagactggg aaaccagaca gctaggaaaa
     4381 ggagcaaaac atagcccagc aacctacaga tgaagaaagt tgagaaatcc atttattcac
     4441 catagagacg caggaatttc aggcaatgca ctaaaatgaa atgggggaaa aaagcttgat
     4501 cagtatggga accatttttg tgcaaaaggg aatattatgg atcagccagt atttctttga
     4561 gctctgcctg tggagtccat ttgaccttta gaaatatgag gtattctgtc agttttatct
     4621 tcttggagaa atttctccta aaatcttgat ttgctttagt ctggactggt tcatagccat
     4681 catcttccat cagtacccca gagattcact ttgtctctta tgtgggatct gtttccagtt
     4741 agatgccatt attttccttt tccttggttt actcttccac atattggtaa agctcttcca
     4801 atagcttttg gaaaggaaaa atgaaaagta aatgttttga atctctgtgt gtttgacaat
     4861 gtctttattt tacccttata cctgattgct gttttggttg gcaaggtata ggattcttta
     4921 gtggtctcca tgcccagttt tgaagacatc tgctagcttt cagtgctgtt gctgtggagt
     4981 ctgaaaatct gtcttctggc ttccagggtg actactggaa attgaatgcc attctgttcc
     5041 ttctcttttg catatataat ccatttttat ctctcttgaa gcttataggt ttatctttgt
     5101 ctcaatgttc tgtccctgtt aagagtccat tttcatcctt tgtactaggt gcctggtggg
     5161 atcattccgt ctgaaactaa tgatttccca tctcttcact gtttctggaa ttcctgtttt
     5221 ccagatgtta gacctccaga atttgatctc taattttcct atcttttctc ttaactttca
     5281 gctctgtctt cttgctagga ccttttccta ggagcatttc tcaatttaat cttccagttc
     5341 atctgttgca ttttattttt ctagtctcat attgtctcat atttttaatt tctaagagct
     5401 ccccttctcc gaatattctt tttttttaat agcatcctat tttggctcat ggttgcagta
     5461 ttttatctcc ttgaagatgt ttgtgtgttt atgtatgtat atgcacacac gtatacatac
     5521 acatacaggc atgcatctct gtattctttc ggcataatct gtgtcctcca gggtttgttt
     5581 ctttgtttcc cctgtatgtt tgttttggtc gttcacatta taggctttcc tcagagttaa
     5641 tggtcttggt agtctactca tatttaagtg tggaacacca aaaagcttac tataagctga
     5701 gagtgtggta aagggctctt tgttttacta tgacctacct gagctatctt gctggggaac
     5761 accctaatgt cagtctcttt ataaagggcc tttcattttg gcctggcaag aaatactctt
     5821 tcatcctcct gcatggaggg caaaaaaaaa tttaaaaatt ggctgctagg gtctgtctgc
     5881 tcacttccct gttttgcaga ccccacactc ttctgcaatt catttcatag ttgtcaagac
     5941 tatacaaatt gtccttttta atgttctctc ttctgctatc cctagttggc agtcttcctc
     6001 tttacaacct gctgaaagtg gaagacctcc agttttcctt taattcctca gcaaaccacc
     6061 aactattata tgtctttttt ccagaacaac ttatttttta actataatta tatgcattta
     6121 tgttagattc actgaaaacc tcatcttgta tggtgctctg taccctatgg gtgctaaata
     6181 aaggcttgct actggcaact gga
//
