LOCUS       NM_014159               8541 bp    mRNA    linear   PRI 12-OCT-2024
DEFINITION  Homo sapiens SET domain containing 2, histone lysine
            methyltransferase (SETD2), transcript variant 1, mRNA.
ACCESSION   NM_014159
VERSION     NM_014159.7
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8541)
  AUTHORS   Zhang,L., Wang,J., Liu,X., Xiao,X., Liu,Y., Huang,Q., Li,J., Li,G.
            and Yang,P.
  TITLE     Regulation of SETD2 maintains immune regulatory function in
            macrophages to suppress airway allergy
  JOURNAL   Immunology 173 (1), 185-195 (2024)
   PUBMED   38859694
  REMARK    GeneRIF: Regulation of SETD2 maintains immune regulatory function
            in macrophages to suppress airway allergy.
REFERENCE   2  (bases 1 to 8541)
  AUTHORS   Xiong,J., Zhu,L., Fu,Y., Ye,Z., Deng,C., Wang,X. and Chen,Y.
  TITLE     Prognostic and therapeutic roles of SETD2 in cutaneous melanoma
  JOURNAL   Aging (Albany NY) 16 (11), 9692-9708 (2024)
   PUBMED   38843391
  REMARK    GeneRIF: Prognostic and therapeutic roles of SETD2 in cutaneous
            melanoma.
REFERENCE   3  (bases 1 to 8541)
  AUTHORS   Chen,Y., Chen,K., Zhu,H., Qin,H., Liu,J. and Cao,X.
  TITLE     Methyltransferase Setd2 prevents T cell-mediated autoimmune
            diseases via phospholipid remodeling
  JOURNAL   Proc Natl Acad Sci U S A 121 (8), e2314561121 (2024)
   PUBMED   38359295
  REMARK    GeneRIF: Methyltransferase Setd2 prevents T cell-mediated
            autoimmune diseases via phospholipid remodeling.
REFERENCE   4  (bases 1 to 8541)
  AUTHORS   Takeda,K., Bastacky,S., Dhir,R., Mohebnasab,M. and
            Quiroga-Garza,G.M.
  TITLE     Morphological characteristics of SETD2-mutated locally advanced
            clear cell renal cell carcinoma: Comparison with BAP1-mutated clear
            cell renal cell carcinoma
  JOURNAL   Ann Diagn Pathol 68, 152223 (2024)
   PUBMED   37976977
  REMARK    GeneRIF: Morphological characteristics of SETD2-mutated locally
            advanced clear cell renal cell carcinoma: Comparison with
            BAP1-mutated clear cell renal cell carcinoma.
REFERENCE   5  (bases 1 to 8541)
  AUTHORS   Ma,C., Liu,M., Feng,W., Rao,H., Zhang,W., Liu,C., Xu,Y., Wang,Z.,
            Teng,Y., Yang,X., Ni,L., Xu,J., Gao,W.Q., Lu,B. and Li,L.
  TITLE     Loss of SETD2 aggravates colorectal cancer progression caused by
            SMAD4 deletion through the RAS/ERK signalling pathway
  JOURNAL   Clin Transl Med 13 (11), e1475 (2023)
   PUBMED   37962020
  REMARK    GeneRIF: Loss of SETD2 aggravates colorectal cancer progression
            caused by SMAD4 deletion through the RAS/ERK signalling pathway.
REFERENCE   6  (bases 1 to 8541)
  AUTHORS   Sun,X.J., Wei,J., Wu,X.Y., Hu,M., Wang,L., Wang,H.H., Zhang,Q.H.,
            Chen,S.J., Huang,Q.H. and Chen,Z.
  TITLE     Identification and characterization of a novel human histone H3
            lysine 36-specific methyltransferase
  JOURNAL   J Biol Chem 280 (42), 35261-35271 (2005)
   PUBMED   16118227
  REMARK    GeneRIF: HYPB HMTase may coordinate histone methylation and
            transcriptional regulation in mammals
            GeneRIF: HYPB protein is a histone H3 lysine 36-specific
            methyltransferase that associates with hyperphosphorylated RNA
            polymerase II.
REFERENCE   7  (bases 1 to 8541)
  AUTHORS   Rega,S., Stiewe,T., Chang,D.I., Pollmeier,B., Esche,H.,
            Bardenheuer,W., Marquitan,G. and Putzer,B.M.
  TITLE     Identification of the full-length huntingtin- interacting protein
            p231HBP/HYPB as a DNA-binding factor
  JOURNAL   Mol Cell Neurosci 18 (1), 68-79 (2001)
   PUBMED   11461154
REFERENCE   8  (bases 1 to 8541)
  AUTHORS   Passani,L.A., Bedford,M.T., Faber,P.W., McGinnis,K.M., Sharp,A.H.,
            Gusella,J.F., Vonsattel,J.P. and MacDonald,M.E.
  TITLE     Huntingtin's WW domain partners in Huntington's disease post-mortem
            brain fulfill genetic criteria for direct involvement in
            Huntington's disease pathogenesis
  JOURNAL   Hum Mol Genet 9 (14), 2175-2182 (2000)
   PUBMED   10958656
REFERENCE   9  (bases 1 to 8541)
  AUTHORS   Faber,P.W., Barnes,G.T., Srinidhi,J., Chen,J., Gusella,J.F. and
            MacDonald,M.E.
  TITLE     Huntingtin interacts with a family of WW domain proteins
  JOURNAL   Hum Mol Genet 7 (9), 1463-1474 (1998)
   PUBMED   9700202
REFERENCE   10 (bases 1 to 8541)
  AUTHORS   Pappas,J. and Rabin,R.
  TITLE     SETD2 Neurodevelopmental Disorders
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   34978780
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC127430.4, DA800082.1,
            BX649110.1, AK127782.1, BC117162.1, AY576987.1, AI671342.1 and
            AC094020.2.
            
            On Feb 25, 2020 this sequence version replaced NM_014159.6.
            
            Summary: Huntington's disease (HD), a neurodegenerative disorder
            characterized by loss of striatal neurons, is caused by an
            expansion of a polyglutamine tract in the HD protein huntingtin.
            This gene encodes a protein belonging to a class of huntingtin
            interacting proteins characterized by WW motifs. This protein is a
            histone methyltransferase that is specific for lysine-36 of histone
            H3, and methylation of this residue is associated with active
            chromatin. This protein also contains a novel transcriptional
            activation domain and has been found associated with
            hyperphosphorylated RNA polymerase II. [provided by RefSeq, Aug
            2008].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038191.2550880.1,
                                           SRR14038195.1819657.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000409792.4/ ENSP00000386759.3
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-136               AC127430.4         179129-179264       c
            137-696             DA800082.1         1-560
            697-3157            BX649110.1         123-2583
            3158-3428           AK127782.1         2439-2709
            3429-4643           BX649110.1         2855-4069
            4644-6073           BC117162.1         3131-4560
            6074-8279           AY576987.1         4447-6652
            8280-8409           AI671342.1         180-309             c
            8410-8410           AC094020.2         175198-175198       c
            8411-8541           AI671342.1         48-178              c
FEATURES             Location/Qualifiers
     source          1..8541
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p21.31"
     gene            1..8541
                     /gene="SETD2"
                     /gene_synonym="HBP231; HIF-1; HIP-1; HSPC069; HYPB; KMT3A;
                     LLS; MRD70; p231HBP; RAPAS; SET2"
                     /note="SET domain containing 2, histone lysine
                     methyltransferase"
                     /db_xref="GeneID:29072"
                     /db_xref="HGNC:HGNC:18420"
                     /db_xref="MIM:612778"
     exon            1..260
                     /gene="SETD2"
                     /gene_synonym="HBP231; HIF-1; HIP-1; HSPC069; HYPB; KMT3A;
                     LLS; MRD70; p231HBP; RAPAS; SET2"
                     /inference="alignment:Splign:2.1.0"
     CDS             190..7884
                     /gene="SETD2"
                     /gene_synonym="HBP231; HIF-1; HIP-1; HSPC069; HYPB; KMT3A;
                     LLS; MRD70; p231HBP; RAPAS; SET2"
                     /EC_number="2.1.1.359"
                     /note="isoform 1 is encoded by transcript variant 1;
                     huntingtin interacting protein 1; histone-lysine
                     N-methyltransferase SETD2; huntingtin yeast partner B;
                     lysine N-methyltransferase 3A; huntingtin-interacting
                     protein B; protein-lysine N-methyltransferase SETD2"
                     /codon_start=1
                     /product="histone-lysine N-methyltransferase SETD2 isoform
                     1"
                     /protein_id="NP_054878.5"
                     /db_xref="CCDS:CCDS2749.2"
                     /db_xref="GeneID:29072"
                     /db_xref="HGNC:HGNC:18420"
                     /db_xref="MIM:612778"
                     /translation="MKQLQPQPPPKMGDFYDPEHPTPEEEENEAKIENVQKTGFIKGP
                     MFKGVASSRFLPKGTKTKVNLEEQGRQKVSFSFSLTKKTLQNRFLTALGNEKQSDTPN
                     PPAVPLQVDSTPKMKMEIGDTLSTAEESSPPKSRVELGKIHFKKHLLHVTSRPLLATT
                     TAVASPPTHAAPLPAVIAESTTVDSPPSSPPPPPPPAQATTLSSPAPVTEPVALPHTP
                     ITVLMAAPVPLPVDVAVRSLKEPPIIIVPESLEADTKQDTISNSLEEHVTQILNEQAD
                     ISSKKEDSHIGKDEEIPDSSKISLSCKKTGSKKKSSQSEGIFLGSESDEDSVRTSSSQ
                     RSHDLKFSASIEKERDFKKSSAPLKSEDLGKPSRSKTDRDDKYFSYSKLERDTRYVSS
                     RCRSERERRRSRSHSRSERGSRTNLSYSRSERSHYYDSDRRYHRSSPYRERTRYSRPY
                     TDNRARESSDSEEEYKKTYSRRTSSHSSSYRDLRTSSYSKSDRDCKTETSYLEMERRG
                     KYSSKLERESKRTSENEAIKRCCSPPNELGFRRGSSYSKHDSSASRYKSTLSKPIPKS
                     DKFKNSFCCTELNEEIKQSHSFSLQTPCSKGSELRMINKNPEREKAGSPAPSNRLNDS
                     PTLKKLDELPIFKSEFITHDSHDSIKELDSLSKVKNDQLRSFCPIELNINGSPGAESD
                     LATFCTSKTDAVLMTSDDSVTGSELSPLVKACMLSSNGFQNISRCKEKDLDDTCMLHK
                     KSESPFRETEPLVSPHQDKLMSMPVMTVDYSKTVVKEPVDTRVSCCKTKDSDIYCTLN
                     DSNPSLCNSEAENIEPSVMKISSNSFMNVHLESKPVICDSRNLTDHSKFACEEYKQSI
                     GSTSSASVNHFDDLYQPIGSSGIASSLQSLPPGIKVDSLTLLKCGENTSPVLDAVLKS
                     KKSSEFLKHAGKETIVEVGSDLPDSGKGFASRENRRNNGLSGKCLQEAQEEGNSILPE
                     RRGRPEISLDERGEGGHVHTSDDSEVVFSSCDLNLTMEDSDGVTYALKCDSSGHAPEI
                     VSTVHEDYSGSSESSNDESDSEDTDSDDSSIPRNRLQSVVVVPKNSTLPMEETSPCSS
                     RSSQSYRHYSDHWEDERLESRRHLYEEKFESIASKACPQTDKFFLHKGTEKNPEISFT
                     QSSRKQIDNRLPELSHPQSDGVDSTSHTDVKSDPLGHPNSEETVKAKIPSRQQEELPI
                     YSSDFEDVPNKSWQQTTFQNRPDSRLGKTELSFSSSCEIPHVDGLHSSEELRNLGWDF
                     SQEKPSTTYQQPDSSYGACGGHKYQQNAEQYGGTRDYWQGNGYWDPRSGRPPGTGVVY
                     DRTQGQVPDSLTDDREEEENWDQQDGSHFSDQSDKFLLSLQKDKGSVQAPEISSNSIK
                     DTLAVNEKKDFSKNLEKNDIKDRGPLKKRRQEIESDSESDGELQDRKKVRVEVEQGET
                     SVPPGSALVGPSCVMDDFRDPQRWKECAKQGKMPCYFDLIEENVYLTERKKNKSHRDI
                     KRMQCECTPLSKDERAQGEIACGEDCLNRLLMIECSSRCPNGDYCSNRRFQRKQHADV
                     EVILTEKKGWGLRAAKDLPSNTFVLEYCGEVLDHKEFKARVKEYARNKNIHYYFMALK
                     NDEIIDATQKGNCSRFMNHSCEPNCETQKWTVNGQLRVGFFTTKLVPSGSELTFDYQF
                     QRYGKEAQKCFCGSANCRGYLGGENRVSIRAAGGKMKKERSRKKDSVDGELEALMENG
                     EGLSDKNQVLSLSRLMVRIETLEQKLTCLELIQNTHSQSCLKSFLERHGLSLLWIWMA
                     ELGDGRESNQKLQEEIIKTLEHLPIPTKNMLEESKVLPIIQRWSQTKTAVPPLSEGDG
                     YSSENTSRAHTPLNTPDPSTKLSTEADTDTPKKLMFRRLKIISENSMDSAISDATSEL
                     EGKDGKEDLDQLENVPVEEEEELQSQQLLPQQLPECKVDSETNIEASKLPTSEPEADA
                     EIEPKESNGTKLEEPINEETPSQDEEEGVSDVESERSQEQPDKTVDISDLATKLLDSW
                     KDLKEVYRIPKKSQTEKENTTTERGRDAVGFRDQTPAPKTPNRSRERDPDKQTQNKEK
                     RKRRSSLSPPSSAYERGTKRPDDRYDTPTSKKKVRIKDRNKLSTEERRKLFEQEVAQR
                     EAQKQQQQMQNLGMTSPLPYDSLGYNAPHHPFAGYPPGYPMQAYVDPSNPNAGKVLLP
                     TPSMDPVCSPAPYDHAQPLVGHSTEPLSAPPPVPVVPHVAAPVEVSSSQYVAQSDGVV
                     HQDSSVAVLPVPAPGPVQGQNYSVWDSNQQSVSVQQQYSPAQSQATIYYQGQTCPTVY
                     GVTSPYSQTTPPIVQSYAQPSLQYIQGQQIFTAHPQGVVVQPAAAVTTIVAPGQPQPL
                     QPSEMVVTNNLLDLPPPSPPKPKTIVLPPNWKTARDPEGKIYYYHVITRQTQWDPPTW
                     ESPGDDASLEHEAEMDLGTPTYDENPMKASKKPKTAEADTSSELAKKSKEVFRKEMSQ
                     FIVQCLNPYRKPDCKVGRITTTEDFKHLARKLTHGVMNKELKYCKNPEDLECNENVKH
                     KTKEYIKKYMQKFGAVYKPKEDTELE"
     exon            261..276
                     /gene="SETD2"
                     /gene_synonym="HBP231; HIF-1; HIP-1; HSPC069; HYPB; KMT3A;
                     LLS; MRD70; p231HBP; RAPAS; SET2"
                     /inference="alignment:Splign:2.1.0"
     exon            277..4643
                     /gene="SETD2"
                     /gene_synonym="HBP231; HIF-1; HIP-1; HSPC069; HYPB; KMT3A;
                     LLS; MRD70; p231HBP; RAPAS; SET2"
                     /inference="alignment:Splign:2.1.0"
     exon            4644..4775
                     /gene="SETD2"
                     /gene_synonym="HBP231; HIF-1; HIP-1; HSPC069; HYPB; KMT3A;
                     LLS; MRD70; p231HBP; RAPAS; SET2"
                     /inference="alignment:Splign:2.1.0"
     exon            4776..4904
                     /gene="SETD2"
                     /gene_synonym="HBP231; HIF-1; HIP-1; HSPC069; HYPB; KMT3A;
                     LLS; MRD70; p231HBP; RAPAS; SET2"
                     /inference="alignment:Splign:2.1.0"
     exon            4905..5028
                     /gene="SETD2"
                     /gene_synonym="HBP231; HIF-1; HIP-1; HSPC069; HYPB; KMT3A;
                     LLS; MRD70; p231HBP; RAPAS; SET2"
                     /inference="alignment:Splign:2.1.0"
     exon            5029..5106
                     /gene="SETD2"
                     /gene_synonym="HBP231; HIF-1; HIP-1; HSPC069; HYPB; KMT3A;
                     LLS; MRD70; p231HBP; RAPAS; SET2"
                     /inference="alignment:Splign:2.1.0"
     exon            5107..5204
                     /gene="SETD2"
                     /gene_synonym="HBP231; HIF-1; HIP-1; HSPC069; HYPB; KMT3A;
                     LLS; MRD70; p231HBP; RAPAS; SET2"
                     /inference="alignment:Splign:2.1.0"
     exon            5205..5331
                     /gene="SETD2"
                     /gene_synonym="HBP231; HIF-1; HIP-1; HSPC069; HYPB; KMT3A;
                     LLS; MRD70; p231HBP; RAPAS; SET2"
                     /inference="alignment:Splign:2.1.0"
     exon            5332..5466
                     /gene="SETD2"
                     /gene_synonym="HBP231; HIF-1; HIP-1; HSPC069; HYPB; KMT3A;
                     LLS; MRD70; p231HBP; RAPAS; SET2"
                     /inference="alignment:Splign:2.1.0"
     exon            5467..5586
                     /gene="SETD2"
                     /gene_synonym="HBP231; HIF-1; HIP-1; HSPC069; HYPB; KMT3A;
                     LLS; MRD70; p231HBP; RAPAS; SET2"
                     /inference="alignment:Splign:2.1.0"
     exon            5587..6249
                     /gene="SETD2"
                     /gene_synonym="HBP231; HIF-1; HIP-1; HSPC069; HYPB; KMT3A;
                     LLS; MRD70; p231HBP; RAPAS; SET2"
                     /inference="alignment:Splign:2.1.0"
     exon            6250..6298
                     /gene="SETD2"
                     /gene_synonym="HBP231; HIF-1; HIP-1; HSPC069; HYPB; KMT3A;
                     LLS; MRD70; p231HBP; RAPAS; SET2"
                     /inference="alignment:Splign:2.1.0"
     exon            6299..6482
                     /gene="SETD2"
                     /gene_synonym="HBP231; HIF-1; HIP-1; HSPC069; HYPB; KMT3A;
                     LLS; MRD70; p231HBP; RAPAS; SET2"
                     /inference="alignment:Splign:2.1.0"
     exon            6483..7152
                     /gene="SETD2"
                     /gene_synonym="HBP231; HIF-1; HIP-1; HSPC069; HYPB; KMT3A;
                     LLS; MRD70; p231HBP; RAPAS; SET2"
                     /inference="alignment:Splign:2.1.0"
     exon            7153..7287
                     /gene="SETD2"
                     /gene_synonym="HBP231; HIF-1; HIP-1; HSPC069; HYPB; KMT3A;
                     LLS; MRD70; p231HBP; RAPAS; SET2"
                     /inference="alignment:Splign:2.1.0"
     exon            7288..7427
                     /gene="SETD2"
                     /gene_synonym="HBP231; HIF-1; HIP-1; HSPC069; HYPB; KMT3A;
                     LLS; MRD70; p231HBP; RAPAS; SET2"
                     /inference="alignment:Splign:2.1.0"
     exon            7428..7539
                     /gene="SETD2"
                     /gene_synonym="HBP231; HIF-1; HIP-1; HSPC069; HYPB; KMT3A;
                     LLS; MRD70; p231HBP; RAPAS; SET2"
                     /inference="alignment:Splign:2.1.0"
     exon            7540..7620
                     /gene="SETD2"
                     /gene_synonym="HBP231; HIF-1; HIP-1; HSPC069; HYPB; KMT3A;
                     LLS; MRD70; p231HBP; RAPAS; SET2"
                     /inference="alignment:Splign:2.1.0"
     exon            7621..7722
                     /gene="SETD2"
                     /gene_synonym="HBP231; HIF-1; HIP-1; HSPC069; HYPB; KMT3A;
                     LLS; MRD70; p231HBP; RAPAS; SET2"
                     /inference="alignment:Splign:2.1.0"
     exon            7723..8541
                     /gene="SETD2"
                     /gene_synonym="HBP231; HIF-1; HIP-1; HSPC069; HYPB; KMT3A;
                     LLS; MRD70; p231HBP; RAPAS; SET2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8523..8528
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SETD2"
                     /gene_synonym="HBP231; HIF-1; HIP-1; HSPC069; HYPB; KMT3A;
                     LLS; MRD70; p231HBP; RAPAS; SET2"
                     /note="hexamer: AATAAA"
     polyA_site      8541
                     /gene="SETD2"
                     /gene_synonym="HBP231; HIF-1; HIP-1; HSPC069; HYPB; KMT3A;
                     LLS; MRD70; p231HBP; RAPAS; SET2"
                     /note="major polyA site"
ORIGIN      
        1 agagagcggc tggcgggcgt ccgagggagg gagggagcga cgagcgaggt agcgacgcgg
       61 gccgcccctg ccgccgccgc cgccgccgcc gccgccgccg cggtcggacc tgcggcctcc
      121 tcccctcccc tcccccgcgt cgccctgctg cggggagggg gctcgcgtcg ccgtctccag
      181 ccgctcccga tgaagcagct gcagccgcag ccgcctccga agatggggga tttctacgac
      241 ccggagcacc cgacccctga agaagaagaa aatgaggcaa agattgaaaa tgtgcagaaa
      301 acaggtttca tcaaaggacc aatgttcaaa ggtgttgctt ctagtcgatt tttgcccaaa
      361 ggcaccaaaa caaaagttaa tttggaagaa cagggacgac agaaggtgtc attcagcttc
      421 agccttacaa agaaaacttt gcagaatagg tttctcactg cacttggcaa tgaaaagcaa
      481 agtgatactc caaaccctcc agctgtacct cttcaggtag actcgactcc taaaatgaaa
      541 atggaaattg gtgatacctt atctactgca gaagaatctt ccccaccaaa gtcaagggtg
      601 gaattgggca aaattcattt taagaaacat ctgcttcatg taacatccag gccactgctg
      661 gctactacca cagcagtagc atctccacct actcatgcag caccattacc agcagtgata
      721 gcagaatcaa caactgtaga ctcaccgccc tcatctccgc ctccaccgcc tccacctgcc
      781 caagccacaa cactctcatc accagcacca gtaacagagc cagtggcctt gccacataca
      841 ccaataacag ttctaatggc agcaccagta cccttaccag tagatgtagc agttagatct
      901 ctgaaagaac caccaattat aattgtacca gaatctttag aagcagatac taagcaggac
      961 actatatcta atagtttaga agaacacgta actcaaatat tgaatgagca agcagatatt
     1021 tcctcaaaaa aagaagattc ccatattggg aaggatgaag aaattccaga tagttctaag
     1081 attagtctga gctgtaaaaa aacaggttct aagaagaaat cctcacaatc tgaaggcatc
     1141 tttcttggtt cagaatctga tgaagattct gtacggactt cttcaagtca aagatcacat
     1201 gatttaaaat tttcagcaag cattgaaaag gaaagagatt ttaaaaagag ctcagcacct
     1261 ttaaaaagtg aggatctagg gaaaccttca cgatctaaaa cagacagaga tgataaatat
     1321 tttagctatt caaaacttga aagagatact cggtatgtat cttcccgatg tagatcagaa
     1381 agagagcgac ggcggagcag atctcactct aggtctgaga gaggctctag aactaattta
     1441 tcctattcca ggtcagaacg atctcattat tatgactctg atcgtcgcta ccataggagc
     1501 tccccttatc gagagaggac gcgctattct cggccataca cagataacag agcacgagag
     1561 agttctgact cagaagaaga gtataagaag acatactcaa ggcgtacctc atctcattcc
     1621 tcttcttaca gagacctaag gacatcatcc tattctaaat ctgatcggga ctgtaaaact
     1681 gagacctctt acttagagat ggaaagaaga ggcaagtatt cttcaaaact agaaagagaa
     1741 tctaaaagga cttcagaaaa tgaagcaatt aaaagatgtt gttctccccc taatgaactg
     1801 ggattccgac gagggtcatc atattctaag catgacagta gtgcttcccg ttataaatct
     1861 accctttcaa aacctatacc caagtctgat aaatttaaaa attctttctg ttgtacagaa
     1921 ttaaatgaag aaatcaaaca gtctcattct tttagtttac agacaccttg ttcaaaaggt
     1981 agtgaattaa gaatgattaa taaaaatcct gaaagagaaa aggctgggtc tccagctcca
     2041 tcaaatcgat taaatgattc acctacttta aaaaagctag atgaattgcc tatttttaag
     2101 tccgaattta taacacatga tagccatgat agtattaagg aattagactc tttatctaaa
     2161 gtgaagaatg atcaattaag aagtttttgt cccatagaat taaatataaa tggatctcct
     2221 ggggcagaat ctgatttggc aacattttgc acttctaaaa ctgatgctgt tttaatgact
     2281 tctgatgata gtgtgactgg atcggaatta tcccctttgg tcaaagcatg catgctttca
     2341 tcaaatggat ttcagaatat tagtaggtgc aaagaaaaag acttggatga tacctgcatg
     2401 ctgcataaga agtcagaaag cccatttaga gaaacagaac ctctggtgtc accacaccaa
     2461 gataaactca tgtctatgcc agttatgact gtggattatt ccaaaacagt agttaaagaa
     2521 ccagttgata cgagggtttc ttgctgcaaa accaaagatt cagacatata ctgtactttg
     2581 aacgatagca acccttcttt gtgtaactct gaagctgaaa atattgagcc ttcagttatg
     2641 aagatttctt caaatagctt tatgaatgtg catttggaat caaaaccagt tatatgtgat
     2701 agtagaaatt tgacagatca ctcaaaattt gcatgtgaag aatataagca gagcatcggt
     2761 agcactagtt cagcttctgt taatcatttt gatgatttat atcaacctat tgggagttca
     2821 ggtattgctt catctcttca gagtcttcca ccaggaataa aggtggacag tctaactctc
     2881 ttgaaatgcg gagagaacac atctccagtt ctggatgcag tgctaaagag taaaaaaagt
     2941 tcagagtttt taaagcatgc agggaaagaa acaatagtag aagtaggtag tgaccttcct
     3001 gattcaggaa agggatttgc ttccagggag aacaggcgta ataatgggtt atctgggaaa
     3061 tgtttgcaag aggctcaaga agaagggaat tccatattgc ctgaaagaag aggaagacca
     3121 gaaatctctt tagatgaaag aggagaagga ggacatgtgc atacttctga tgactcagaa
     3181 gttgtatttt cttcttgtga tttgaattta accatggaag acagtgatgg tgtaacttat
     3241 gcattaaagt gtgacagtag tggtcatgcc ccagaaattg tgtctacagt tcatgaagat
     3301 tattctggct cttctgaaag ttcaaatgat gaaagtgatt cagaagatac agattcggat
     3361 gatagcagta ttccaagaaa ccgtctccag tctgttgtgg ttgtgccaaa gaattctact
     3421 ttgcccatgg aagaaacaag tccttgttct tctcggagca gtcaaagtta tagacactat
     3481 tctgaccatt gggaagatga gagattggag tcaaggagac atttgtatga ggaaaaattt
     3541 gaaagtatag caagtaaagc ctgtcctcaa actgataagt ttttccttca taaaggaaca
     3601 gagaagaatc cggaaatttc ttttacacag tccagtagaa aacaaataga taatcgcctg
     3661 cctgaacttt ctcatcctca gagtgatggg gttgatagta caagtcatac agatgtgaaa
     3721 tctgaccctc tgggtcaccc aaattcagag gaaaccgtga aagccaaaat accttctagg
     3781 cagcaagaag agctgccaat ttattcttct gattttgaag atgtcccaaa taagtcttgg
     3841 caacagacca ctttccaaaa caggccagat agtagactgg gaaaaacaga attgagtttt
     3901 tcttcctctt gtgagatacc acatgtggat ggcttgcact catcagaaga gctcagaaac
     3961 ttaggttggg acttctctca agaaaagcct tctaccacgt atcagcaacc tgacagtagc
     4021 tatggagctt gtggtggaca caagtatcag caaaatgcag aacagtatgg tgggacacgt
     4081 gattactggc aaggcaatgg ttactgggat ccaagatcag gtagacctcc tggaactggg
     4141 gttgtgtatg atcgaactca aggacaagta ccagattccc taacagatga tcgtgaagaa
     4201 gaggagaatt gggatcaaca ggatggatcc catttttcag accagtccga taaatttctt
     4261 ctatcccttc agaaagacaa ggggtcagtg caagcacctg aaataagcag caattccatt
     4321 aaggacactt tagctgtgaa tgaaaagaaa gatttttcaa aaaacttaga aaaaaatgat
     4381 atcaaagata gagggcctct taaaaaaagg aggcaggaaa tagagagtga ttctgaaagt
     4441 gatggtgagc ttcaggacag aaagaaagtt agagtggagg tagagcaggg agagacatca
     4501 gtgcccccag gttcagcact ggttgggccc tcctgtgtca tggatgactt cagggaccca
     4561 cagcgatgga aggaatgtgc caagcaaggg aaaatgccat gttactttga tcttattgaa
     4621 gaaaatgttt atttaacaga aagaaagaag aataaatctc atcgagatat taagcgaatg
     4681 cagtgtgagt gtacacctct ttctaaagat gaaagagctc aaggtgaaat agcatgtggg
     4741 gaagattgtc ttaatcgtct tctcatgatt gaatgttctt ctcggtgtcc aaatggggat
     4801 tattgttcca atagacggtt tcagagaaaa cagcatgcag atgtggaagt catactcaca
     4861 gaaaagaaag gctggggctt gagagctgcc aaagaccttc cttcgaacac ctttgtccta
     4921 gaatattgtg gagaggtact cgatcataaa gagtttaaag ctcgagtgaa ggagtatgca
     4981 cgaaacaaaa acatccatta ctatttcatg gccctgaaga atgatgagat aatagatgcc
     5041 actcaaaaag gaaattgctc tcgtttcatg aatcacagct gtgaaccaaa ttgtgaaacc
     5101 caaaaatgga ctgtgaacgg acaactgagg gttgggtttt ttaccaccaa actggttcct
     5161 tcaggctcag agttaacgtt tgactatcag ttccagagat atggaaaaga agcccagaaa
     5221 tgtttctgcg gatcagccaa ttgccggggt tacctgggag gagaaaacag agtcagcatc
     5281 agagcagcag gagggaaaat gaagaaggaa cgatctcgta agaaggattc agtggatgga
     5341 gagctagaag ctctgatgga aaatggtgag ggtctctctg ataaaaacca ggtgctcagc
     5401 ttatcccggc taatggttag aattgaaact ttggagcaga aacttacctg tctggaactc
     5461 atacagaaca cacactcaca gtcctgcctg aagtcctttc tggaacgtca tgggctgtct
     5521 ttgttgtgga tctggatggc agagctaggt gacggccggg aaagtaacca gaagcttcag
     5581 gaagagatta taaagacttt ggaacacttg cccattccta ctaaaaatat gttggaggaa
     5641 agcaaagtac ttccaattat tcaacgctgg tctcagacta agactgctgt ccctccgttg
     5701 agtgaaggag atgggtattc tagtgagaat acatcgcgtg ctcatacacc actcaacaca
     5761 cctgatcctt ccaccaagct gagcacagaa gctgacacag acactcccaa gaaactaatg
     5821 tttcgcagac tgaaaattat aagtgaaaat agcatggaca gtgcaatctc tgatgcaacc
     5881 agtgagctag aaggcaagga tggcaaagag gatcttgatc aattagaaaa tgtccctgta
     5941 gaggaagagg aagaattgca gtcacaacag ctactcccac aacagctgcc tgaatgcaaa
     6001 gttgatagtg aaaccaacat agaagctagt aagctaccta catctgaacc agaagctgac
     6061 gctgaaatag agcccaaaga gagcaacggc acaaaactag aagaacctat taatgaagaa
     6121 acaccatccc aagatgaaga ggagggtgtg tctgatgtgg agagtgaaag gagccaagaa
     6181 cagccagata aaacagtgga tataagtgat ttggccacca aactcctgga cagttggaaa
     6241 gacctaaagg aggtatatcg aattccaaag aaaagtcaaa ctgaaaagga aaacacaaca
     6301 actgaacgag gaagggatgc tgttggcttc agagatcaaa cacctgcccc gaagactcct
     6361 aataggtcaa gagagagaga cccagacaag caaactcaaa ataaagagaa aaggaaacga
     6421 agaagctccc tctcaccacc ctcttctgcc tatgagcggg gaacaaaaag gccagatgac
     6481 agatatgata caccaacttc taaaaagaaa gtacgaatta aagaccgcaa taaactttct
     6541 acagaggaac gccggaagtt gtttgagcaa gaggtggctc aacgggaggc tcagaaacaa
     6601 cagcaacaga tgcagaacct gggaatgaca tcaccactgc cctatgactc tcttggttat
     6661 aatgccccgc atcatccctt tgctggttac ccaccaggtt atcccatgca ggcctatgtg
     6721 gatcccagca accctaatgc tggaaaggtg ctcctgccca cacccagcat ggacccagtg
     6781 tgttctcctg ctccttatga tcatgctcag cccttggtgg gacattctac agaacccctt
     6841 tctgcccctc caccagtacc agtggtgcca catgtggcag ctcctgtgga agtttccagt
     6901 tcccagtatg tggcccagag tgatggtgta gtacaccaag actccagcgt tgctgtcttg
     6961 ccagtgccgg cccccggccc agttcaggga cagaattata gtgtttggga ttcaaaccaa
     7021 cagtctgtca gtgtacagca gcagtactct cctgcacagt ctcaagcaac catatattat
     7081 caaggacaga catgtccaac agtctatggt gtgacatcac cttattcaca gacaactcca
     7141 ccaattgtac agagttatgc ccagccaagt cttcagtata tccaggggca acagattttc
     7201 acagctcatc cacaaggagt ggtggtacag ccagccgcag cagtgactac aatagttgca
     7261 ccagggcagc ctcagccctt gcagccatct gaaatggttg tgacaaataa tctcttggat
     7321 ctgccgcccc cctctcctcc caaaccaaaa accattgtct tacctcccaa ctggaagaca
     7381 gctcgagatc cagaagggaa gatttattac taccatgtga tcacaaggca gactcagtgg
     7441 gatcctccta cttgggaaag cccaggagat gatgccagcc ttgagcatga agctgagatg
     7501 gacctgggaa ctccaacata tgatgaaaac cccatgaagg cctcgaaaaa gcccaagaca
     7561 gcagaagcag acacctccag tgaactagca aagaaaagca aagaagtatt cagaaaagag
     7621 atgtcccagt tcatcgtcca gtgcctgaac ccttaccgga aacctgactg caaagtggga
     7681 agaattacca caactgaaga ctttaaacat ctggctcgca agctgactca cggtgttatg
     7741 aataaggagc tgaagtactg taagaatcct gaggacctgg agtgcaatga gaatgtgaaa
     7801 cacaaaacca aggagtacat taagaagtac atgcagaagt ttggggctgt ttacaaaccc
     7861 aaagaggaca ctgaattaga gtgactgttg ggccagggtg ggaggatggg tggtcaggta
     7921 agacagactc tagggagagg aaatcctgtg ggcctttctg tcccacccct gtcagcactg
     7981 tgctactgat gatacatcac cctggggaat tcaaccctgc agatgtcaac tgaaggccac
     8041 aaaaatgaac tccatctaca agtgattacc tagttgtgag ctgttggcat gtggttagaa
     8101 gccatcagag gtgcaagggc ttagaaaaga ccctggccag acctgactcc actcttaaac
     8161 ctgggtcttc tccttggcgg tgctgtcagc gcacagaccc atgcgcatcc ccacccacaa
     8221 ccctttaccc tgatgatctg tattatattt taatgtatat gtgaatatat tgaaaataat
     8281 ttgttttttc ctggtttttg tttggttttc gttttgcttt tagcctctac atgctaggat
     8341 cacaggaaga ctttgtaagg acagtttaag ttctcctgca aggtttaatt tgttatcatg
     8401 taaatattct aaagcaggct gccttgtggt tttggccagc cttgtgctat gttgataaga
     8461 ttgatttact gcttaaaatc actttacttt atccaatttt tactgaactt tttatgtaaa
     8521 aaaataaaat caattaaaga a
//
