LOCUS       NM_178123              10671 bp    mRNA    linear   PRI 21-NOV-2024
DEFINITION  Homo sapiens SEC14 and spectrin domain containing 1 (SESTD1), mRNA.
ACCESSION   NM_178123
VERSION     NM_178123.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10671)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 10671)
  AUTHORS   Fragoza,R., Das,J., Wierbowski,S.D., Liang,J., Tran,T.N., Liang,S.,
            Beltran,J.F., Rivera-Erick,C.A., Ye,K., Wang,T.Y., Yao,L., Mort,M.,
            Stenson,P.D., Cooper,D.N., Wei,X., Keinan,A., Schimenti,J.C.,
            Clark,A.G. and Yu,H.
  TITLE     Extensive disruption of protein interactions by genetic variants
            across the allele frequency spectrum in human populations
  JOURNAL   Nat Commun 10 (1), 4141 (2019)
   PUBMED   31515488
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 10671)
  AUTHORS   Fujiwara,S., Matsui,T.S., Ohashi,K., Deguchi,S. and Mizuno,K.
  TITLE     Solo, a RhoA-targeting guanine nucleotide exchange factor, is
            critical for hemidesmosome formation and acinar development in
            epithelial cells
  JOURNAL   PLoS One 13 (4), e0195124 (2018)
   PUBMED   29672603
  REMARK    GeneRIF: Solo plays a crucial role in HD formation and acinar
            development in epithelial cells by regulating mechanical
            force-induced RhoA activation and keratin filament organization.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 10671)
  AUTHORS   Fujiwara,S., Ohashi,K., Mashiko,T., Kondo,H. and Mizuno,K.
  TITLE     Interplay between Solo and keratin filaments is crucial for
            mechanical force-induced stress fiber reinforcement
  JOURNAL   Mol Biol Cell 27 (6), 954-966 (2016)
   PUBMED   26823019
  REMARK    GeneRIF: The interplay between Solo protein and keratin 8/keratin
            18 filaments plays a crucial role in tensile force-induced RhoA
            activation and consequent actin cytoskeletal reinforcement in
            endothelial cells.
REFERENCE   5  (bases 1 to 10671)
  AUTHORS   Slonchak,A., Shannon,R.P., Pali,G. and Khromykh,A.A.
  TITLE     Human MicroRNA miR-532-5p Exhibits Antiviral Activity against West
            Nile Virus via Suppression of Host Genes SESTD1 and TAB3 Required
            for Virus Replication
  JOURNAL   J Virol 90 (5), 2388-2402 (2015)
   PUBMED   26676784
  REMARK    GeneRIF: Upregulation of miR-532-5p and subsequent suppression of
            the SESTD1 and TAB3 genes represent an antiviral response aimed at
            limiting West Nile virus infection.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 10671)
  AUTHORS   Kamboh,M.I., Wang,X., Kao,A.H., Barmada,M.M., Clarke,A.,
            Ramsey-Goldman,R., Manzi,S. and Demirci,F.Y.
  TITLE     Genome-wide association study of antiphospholipid antibodies
  JOURNAL   Autoimmune Dis 2013, 761046 (2013)
   PUBMED   23509613
REFERENCE   7  (bases 1 to 10671)
  AUTHORS   Miehe,S., Bieberstein,A., Arnould,I., Ihdene,O., Rutten,H. and
            Strubing,C.
  TITLE     The phospholipid-binding protein SESTD1 is a novel regulator of the
            transient receptor potential channels TRPC4 and TRPC5
  JOURNAL   J Biol Chem 285 (16), 12426-12434 (2010)
   PUBMED   20164195
  REMARK    GeneRIF: SESTD1 was found to associate with TRPC4 and TRPC5 via the
            channel's calmodulin- and inositol 1,4,5-trisphosphate
            receptor-binding domain.
REFERENCE   8  (bases 1 to 10671)
  AUTHORS   Trynka,G., Zhernakova,A., Romanos,J., Franke,L., Hunt,K.A.,
            Turner,G., Bruinenberg,M., Heap,G.A., Platteel,M., Ryan,A.W., de
            Kovel,C., Holmes,G.K., Howdle,P.D., Walters,J.R., Sanders,D.S.,
            Mulder,C.J., Mearin,M.L., Verbeek,W.H., Trimble,V., Stevens,F.M.,
            Kelleher,D., Barisani,D., Bardella,M.T., McManus,R., van Heel,D.A.
            and Wijmenga,C.
  TITLE     Coeliac disease-associated risk variants in TNFAIP3 and REL
            implicate altered NF-kappaB signalling
  JOURNAL   Gut 58 (8), 1078-1083 (2009)
   PUBMED   19240061
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   9  (bases 1 to 10671)
  AUTHORS   Sato,T. and Mishina,M.
  TITLE     Representational difference analysis, high-resolution physical
            mapping, and transcript identification of the zebrafish genomic
            region for a motor behavior
  JOURNAL   Genomics 82 (2), 218-229 (2003)
   PUBMED   12837271
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC093911.2, BC047578.1, AY209190.1, AK096232.1 and AC016755.6.
            
            On Nov 22, 2018 this sequence version replaced NM_178123.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038194.3673092.1, BC047578.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000428443.8/ ENSP00000415332.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-209               AC093911.2         128522-128730       c
            210-421             BC047578.1         150-361
            422-422             AC093911.2         47079-47079         c
            423-1882            AY209190.1         111-1570
            1883-2786           AK096232.1         1652-2555
            2787-10657          AC016755.6         10352-18222         c
            10658-10671         AC016755.6         10338-10351         c
FEATURES             Location/Qualifiers
     source          1..10671
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q31.2"
     gene            1..10671
                     /gene="SESTD1"
                     /gene_synonym="Solo"
                     /note="SEC14 and spectrin domain containing 1"
                     /db_xref="GeneID:91404"
                     /db_xref="HGNC:HGNC:18379"
                     /db_xref="MIM:621011"
     exon            1..334
                     /gene="SESTD1"
                     /gene_synonym="Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            335..414
                     /gene="SESTD1"
                     /gene_synonym="Solo"
                     /inference="alignment:Splign:2.1.0"
     CDS             360..2450
                     /gene="SESTD1"
                     /gene_synonym="Solo"
                     /note="huntingtin-interacting protein-like protein;
                     protein Solo; SEC14 and spectrin domains 1"
                     /codon_start=1
                     /product="SEC14 domain and spectrin repeat-containing
                     protein 1"
                     /protein_id="NP_835224.3"
                     /db_xref="CCDS:CCDS33338.1"
                     /db_xref="GeneID:91404"
                     /db_xref="HGNC:HGNC:18379"
                     /db_xref="MIM:621011"
                     /translation="MEASVILPILKKKLAFLSGGKDRRSGLILTIPLCLEQTNMDELS
                     VTLDYLLSIPSEKCKARGFTVIVDGRKSQWNVVKTVVVMLQNVVPAEVSLVCVVKPDE
                     FWDKKVTHFCFWKEKDRLGFEVILVSANKLTRYIEPCQLTEDFGGSLTYDHMDWLNKR
                     LVFEKFTKESTSLLDELALINNGSDKGNQQEKERSVDLNFLPSVDPETVLQTGHELLS
                     ELQQRRFNGSDGGVSWSPMDDELLAQPQVMKLLDSLREQYTRYQEVCRQRSKRTQLEE
                     IQQKVMQVVNWLEGPGSEQLRAQWGIGDSIRASQALQQKHEEIESQHSEWFAVYVELN
                     QQIAALLNAGDEEDLVELKSLQQQLSDVCYRQASQLEFRQNLLQAALEFHGVAQDLSQ
                     QLDGLLGMLCVDVAPADGASIQQTLKLLEEKLKSVDVGLQGLREKGQGLLDQISNQAS
                     WAYGKDVTIENKENVDHIQGVMEDMQLRKQRCEDMVDVRRLKMLQMVQLFKCEEDAAQ
                     AVEWLSELLDALLKTHIRLGDDAQETKVLLEKHRKFVDVAQSTYDYGRQLLQATVVLC
                     QSLRCTSRSSGDTLPRLNRVWKQFTIASEERVHRLEMAIAFHSNAEKILQDCPEEPEA
                     INDEEQFDEIEAVGKSLLDRLTVPVVYPDGTEQYFGSPSDMASTAENIRDRMKLVNLK
                     RQQLRHPEMVTTES"
     exon            415..523
                     /gene="SESTD1"
                     /gene_synonym="Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            524..614
                     /gene="SESTD1"
                     /gene_synonym="Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            615..728
                     /gene="SESTD1"
                     /gene_synonym="Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            729..842
                     /gene="SESTD1"
                     /gene_synonym="Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            843..940
                     /gene="SESTD1"
                     /gene_synonym="Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            941..996
                     /gene="SESTD1"
                     /gene_synonym="Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            997..1208
                     /gene="SESTD1"
                     /gene_synonym="Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            1209..1331
                     /gene="SESTD1"
                     /gene_synonym="Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            1332..1526
                     /gene="SESTD1"
                     /gene_synonym="Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            1527..1641
                     /gene="SESTD1"
                     /gene_synonym="Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            1642..1801
                     /gene="SESTD1"
                     /gene_synonym="Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            1802..1883
                     /gene="SESTD1"
                     /gene_synonym="Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            1884..2006
                     /gene="SESTD1"
                     /gene_synonym="Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            2007..2198
                     /gene="SESTD1"
                     /gene_synonym="Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            2199..2320
                     /gene="SESTD1"
                     /gene_synonym="Solo"
                     /inference="alignment:Splign:2.1.0"
     exon            2321..10671
                     /gene="SESTD1"
                     /gene_synonym="Solo"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3021..3026
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SESTD1"
                     /gene_synonym="Solo"
                     /note="hexamer: AATAAA"
     polyA_site      3046
                     /gene="SESTD1"
                     /gene_synonym="Solo"
                     /note="major polyA site"
     regulatory      3199..3204
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SESTD1"
                     /gene_synonym="Solo"
                     /note="hexamer: AATAAG"
     polyA_site      3223
                     /gene="SESTD1"
                     /gene_synonym="Solo"
     regulatory      9810..9815
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SESTD1"
                     /gene_synonym="Solo"
                     /note="hexamer: AATAAA"
     polyA_site      9832
                     /gene="SESTD1"
                     /gene_synonym="Solo"
     regulatory      9901..9906
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SESTD1"
                     /gene_synonym="Solo"
                     /note="hexamer: AATAAA"
     regulatory      9914..9919
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SESTD1"
                     /gene_synonym="Solo"
                     /note="hexamer: CATAAA"
     polyA_site      9924
                     /gene="SESTD1"
                     /gene_synonym="Solo"
     polyA_site      9935
                     /gene="SESTD1"
                     /gene_synonym="Solo"
     regulatory      10047..10052
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SESTD1"
                     /gene_synonym="Solo"
                     /note="hexamer: ATTAAA"
     polyA_site      10070
                     /gene="SESTD1"
                     /gene_synonym="Solo"
     regulatory      10634..10639
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SESTD1"
                     /gene_synonym="Solo"
                     /note="hexamer: AATAAA"
     polyA_site      10671
                     /gene="SESTD1"
                     /gene_synonym="Solo"
ORIGIN      
        1 cttcctcctt cccgcgccgg gcgggttgct gtaccgccgt cacccgggcg cgcgcggggt
       61 cgcgcggacg cggctcgggc cgcgggtgcc gagaggtcgc cgggccaccc gcacccccgc
      121 tccccgcctg acgcacggag gggccgggac gcgatttgcc gcgagtgact ggagccgcag
      181 ccgtcgccgc tgctgccgcc gccgcccggg cccgctccgc ccccagcccg ccttcctccc
      241 gccgcgccct ccgcctccgc ccgcacctcg ctagcgttcc cctgtcttcc ccaacgcccc
      301 ggagccgccg gccgctagcg tcagcgccag ccagaattaa ggaagttcac tggagtaaaa
      361 tggaggcctc agtaatatta cccattctga agaaaaaact agccttcctt tcaggaggaa
      421 aagacagacg gagtggcctc attttgacaa ttccattatg cctcgaacag acaaatatgg
      481 atgagctgag tgtcacctta gactacctac tcagcattcc aagtgagaag tgtaaggcta
      541 gaggatttac cgtgattgtg gatggcagaa aatcacagtg gaatgtggtg aaaacagtag
      601 tcgtaatgct acagaatgtt gttccagctg aggtgtccct tgtttgtgtg gtaaagccag
      661 atgaattctg ggataagaaa gtaacgcatt tttgtttttg gaaggagaag gatagacttg
      721 gctttgaggt tattttagtg tccgccaaca aattgactcg ttatatagaa ccatgccaat
      781 taacagaaga ttttggtggg agtctcacct atgatcacat ggactggtta aataagaggc
      841 tggtttttga gaagtttaca aaggaatcta catcattatt agatgaactt gctttgatta
      901 acaatggaag tgataaagga aatcagcaag agaaagaaag gtctgtggat ttaaactttc
      961 ttccatcggt tgatcctgaa acagttcttc agacagggca tgaattgttg tccgaattac
     1021 agcagcgtcg atttaatggc tcagacggag gggtttcatg gtctcctatg gatgatgaac
     1081 ttcttgcaca gccacaggtt atgaaattat tagattcact ccgagagcaa tatacccgct
     1141 accaggaagt ttgtaggcaa cgtagcaagc gcacacagtt agaagagatt caacagaagg
     1201 taatgcaggt ggtgaactgg ctagaagggc ctggatcaga acaactaaga gcccagtggg
     1261 gcattggaga ctccattagg gcctcccagg ccctacagca gaaacacgaa gagattgaga
     1321 gccagcacag tgaatggttt gcagtgtatg tggaacttaa tcagcaaatt gcagcactct
     1381 tgaatgctgg cgatgaggaa gatcttgtgg aactaaagtc actgcagcaa caacttagtg
     1441 atgtttgtta tcgacaggcc agtcagctgg aatttaggca aaatctctta caagcagctc
     1501 ttgaatttca tggtgttgcc caagatttgt ctcagcagtt ggatggctta ttagggatgt
     1561 tgtgcgtaga tgtagcacca gctgatggag catcgattca gcaaacttta aaactgcttg
     1621 aagagaagct gaaaagtgtt gatgtgggat tgcaaggttt gcgtgaaaaa ggtcaaggtc
     1681 tcctggatca gatctccaat caggcatcct gggcctatgg aaaggatgta accattgaaa
     1741 ataaagaaaa tgtggaccac atacaaggag tgatggaaga tatgcagctt agaaaacaaa
     1801 gatgtgaaga catggtagat gtgcgaaggt taaagatgct tcagatggtg cagttgttta
     1861 aatgtgaaga agatgctgcc caggcagtag aatggctaag tgaacttctg gatgctctgc
     1921 ttaagactca catcagattg ggcgatgatg ctcaagaaac gaaagttttg ctggaaaagc
     1981 atagaaaatt tgttgatgtt gcacagagca cttatgacta tggcaggcag ttgctacagg
     2041 ccacagttgt gttatgccaa tctttgcgct gcacttctcg gtcatctggg gatacacttc
     2101 ctcgactgaa cagagtatgg aaacaattta caatagcatc tgaagagaga gtacatagat
     2161 tggaaatggc tattgcattt cactcaaatg ctgaaaagat tttgcaggac tgtccagaag
     2221 agcctgaagc tattaatgat gaggagcaat ttgatgaaat tgaagcagtt gggaaatcac
     2281 ttttggatag attaactgtt ccagtagttt atcctgatgg aaccgaacaa tattttggga
     2341 gtccaagtga catggcttct actgcagaaa acatcagaga caggatgaaa ctagttaatc
     2401 tcaaaaggca gcagctgaga catcctgaaa tggtgaccac agagagctaa tagctaccag
     2461 ctacctacag atttgcagtt cataatcccg catgttgtca acatactaca gcattagcca
     2521 ccacacctta agatgcattt cacagccaaa ataagtctca tttcttttca tgacacattt
     2581 ctctttacat gttaacacct tgctactacc aaggcataat tacttaacat gcttcgaggc
     2641 tgtagattcc aagtatctta aaagaaggaa ctataaacat tgcactgaaa acttgcttta
     2701 aagctttacc tgacctgtca gtttgtagac aaacaactga taataagctt tgaatggtgc
     2761 taataagagt aggaattctc tctattaaaa agaaaaaaaa aagttgccct tcctccacag
     2821 gtgatttagt aaatttagac agtagttaaa ctcttgttag tagacagtgg tgtcctcaaa
     2881 attttacttt gtaattcttc agaattgatt atttttattg tgtcaataca gagaaagcct
     2941 ttcagatctt tgatatatca tagtcattaa aagacctttt cctatttgta ttgataatgt
     3001 attaaaagtt gtttgtgctt aataaaagac ttctttaaac atcttattta atttagtagt
     3061 tacatcctat ttccaaacat gagtgcctta tttaaaaggg cattcttagg actgtgagga
     3121 tggtttaata tttgtttttt catggtggtt gcatgtattt tagacaggaa atacatatgt
     3181 aagcatgtgt atataataaa taagcatgtt ttatcatgaa aaattattgt gaacaattta
     3241 gatctttaag aacttattaa taatggaata ctatttctaa tttttctctt tttcaacttg
     3301 aaaaatattc tcaaaattat taactaccct gaagatactt tgtctttagg ggaggagggc
     3361 tgaggaagaa ggcatacata attacttcag tgtaatcctt tatatcagag taatctttca
     3421 ggaactaaaa tagcaattgt taataaaatt tagtttctca ttatagtcta aaagcaaaat
     3481 aaattctgaa gtagtccaat aacagaactg tatgacccct ttagaaataa aatttatcat
     3541 gaaatgctag ttcttttaag tttgtattaa gtttaaatgg taaaatgcat ataatttaaa
     3601 ttttatgtat ttttatttca gaacattttt taaaatatta aaaattattt tttagtcttg
     3661 tagtcattac aaaattataa acgagttgta tactggttcc tttttgaggt ctacaaacat
     3721 gctcgtgtat cttccaatgc aaaaaaaaaa cccctccctt ttaacctgct agtttttcca
     3781 gccttcttca ctgactgaca gacttcttgg agaaatccac atccagtgcc cctacatcct
     3841 catcactcac tactcagcac cttgcagtct ggttttacac agcactctac tgaaactgtt
     3901 cttttgaaag tcagcaatgg cctcccagtt accaaactga atggtttttt tctccgtctg
     3961 aatcccttac ccttttctct gcaacattta caccattgag caacctttcc tagacagtgt
     4021 ctccttcctt gaagatgcta tcctctgaaa taactgtgag tacagtcgca taaccctaat
     4081 ttaagatgtg cctgctgaac atgtgctctc tcagatcctg tcctcagaat gcttttggct
     4141 cttttctcgg tatctcatcc ttagagtttt tattttatac caatgttctg gattacatac
     4201 ctggtccaga aatctagact tgtacttctt tttaattgcc tagttgacat ttcaaccttg
     4261 attaaagatt gacaatattt atcacctttt cctcctggaa caatttaggt taatggtgca
     4321 gccttttttc ctagtcaaac atgccaggaa gttcagggca aagttcaact gctccctctt
     4381 gtcttatgtg gtcactgctt ctgagaacat ccaggaagtt ctttctatac ttgaatcatc
     4441 ttctcagtcc tttggggctt tactgctatg agtatgaact tttgtgatta tttatttcag
     4501 tcttcatgat ggtgctatca cattctacag ttgtttatgt ctgtacccag taagctcttt
     4561 tgagggaagg aatcttgtct tctcctattc ccagtcttgc tcatgcataa cagatgccca
     4621 ttcctcctaa atatgaatgc tgtattatag gcattagtaa taatccctgt tacattgcag
     4681 tctgaagtat gttttaaaca tactttttta aagttagaac tgacttacaa gtgaatctat
     4741 tcttagattc ttagatacta actactttag atatttagtt atactcaata actaaatatc
     4801 aatactcaat atctgtttct tctgaatttc agagtagtta gaatgttttt cttaagactt
     4861 tgctagaaag gaactggttt gttagtaatt ctgccatcaa ctcatggagt tataattgcc
     4921 aaatgagtta atttttatat accttaagct atcttgccta caaaaagtaa aataaggcag
     4981 gattcataaa tgttacttgt aaattgagtg tttcacatga ggccaagtat gattttaaca
     5041 taatgaatat cattgctcct catctgtaaa tttgctgctg ctttcatatg tgtatctgta
     5101 catagagtta tgaatgaaaa tccagtaatg ccctaaagca actttcttcc atagggtcaa
     5161 agctcactta aggtaaacgt gtgaaaaaag tgactgcagt gtgtttgaaa ccacaagctg
     5221 tttctatgtc ataaattaac atcagcttgc aagtctctga aagaaccact catttgtaat
     5281 tggtatatat gtcacaacct cccacattcc tttagcccat ctcagggaat tattgaaacg
     5341 ctggcagggt tagtttcatt ttgttttggt ttttttgggt ttttttgttt gtttgaggat
     5401 tttttttttc ttttatcatt tgtttgctcc tgtgtaagct taatatataa tggcagtttg
     5461 gcatctgttc ttcgcacatg tctaacccag cagagttatg caaaaatgat gacttcattt
     5521 taggtcaaat tggctttgtt cccagtcctc attgttggtc actttgttca gttctggtga
     5581 ctcattggtg atgttgatga aactgctcag cctgccagct gggatattga tggcaggtct
     5641 aggcttcatt gttaggtaga agattgaata cttctcactt tatacaaatt tacattggct
     5701 attgctccca tgtgttagct agtcttaaca tgtttgttat ttcgttatct tattagctga
     5761 catacggagt ggtattatta gtgtcgtact ccttagcgtg agcatgacaa ttaagttacc
     5821 ttactaacag tgatgagtaa accatactct gatctaaacc cattgttacc aatgatgtga
     5881 ttatgaagtt agctgaatct atggtctaca tttgaagaaa tagttcagaa ttctctacta
     5941 cattgtaagt tacttgctaa tagagattgg gcattattat gtactactcc ccttctcaca
     6001 tcctacccaa agtacacaag gccgtagtaa aatactgttg ctactaaaaa ctaaacatac
     6061 tgtggttgct aatgtccttt acatcttcag cctataggga atggtacagg cagaaacaag
     6121 caccattaag tatattatca tcagtagtga gatctgtatg attaggatat ctttgcttaa
     6181 gcccccaaag gaaaaattct ctagcattaa aaacatggct ggaacatagc taagcagtta
     6241 acctaattag tagacttatt gttaacgttc agtcctaaaa atgaaaagct tcagttggga
     6301 gtagaaaata tactatgctg gtatatagag caacaaatac cctgatgaga taaaatgaag
     6361 tagaaactta ccatttgaca ttatttgact ttcatacatg ctactgaaaa gtctaggttt
     6421 agctaagaag cataaataat ggcagtactt gtagaggaaa gaacatacca gctaaaacaa
     6481 gaactgtaat gaatataatg gaaagtgttt ggagcttgaa tacttggctt caaaaatctg
     6541 gctttgccat ttactgttaa aaaaatatct gagaatttat ctcaggctgt aaaacagaga
     6601 agtcatacca atttcaagaa taaaatatga tataaagaat tgttgaagcc aaaatatcca
     6661 gaaataaaca gaatggaagg tatcattgct ataattatct tttcagaata aaaaatagag
     6721 tattctttat cctttctgtt gtctcacatg acaagcatgg gatggtgagg aaatggcata
     6781 ttgcctatgt tcgccgcccc acccccttag ttccaccacc ccttcaccgt cacccccagt
     6841 tcactagtgt atcagaatgc atcttcagaa ttacacactg aagatacaaa aggtccagtt
     6901 ggtaagctca tcagtaaata acaaggcttt gaaatctgac aaattagcta tttttgtagg
     6961 aagataatag aacagcaaga tggaagacaa gtgatgagta tcatctgttt tgaggttgac
     7021 aaggtgtgac ataaccctgt taatagacac aagtaaaaga aaatcatact aggattggat
     7081 attagtgagc gagcaccgac cagtagaaac aacaacagca taaatctgtt atttaagtat
     7141 cccaataatg aggttaaaaa aaaaaaaaaa acagaacaaa gcatgaaaag accacaatta
     7201 gtgctggcta tgcttagaga ggagcagtct cttggtaaac tcaggcagtg atgatgaaca
     7261 gaaaatggcc gactgatact gtaatagagg catagaacta aggacactcc gtgagctggc
     7321 ataaatacag gggagtcttt aagactgaaa aaagttcatg ggtatgacac ttaatatgac
     7381 tcaacattac tgcagaattt taaagcaaac atgaaagctt gagaataaac actagtccct
     7441 ctacttcaag aaaaagcaga acctgtacta gaacatgaag aacttagaga tcaaaagaca
     7501 gccagaacta tgatgaaata ttttgaaagc agtgggcaca tttttaaaga ttttttttgg
     7561 agaactttat gaggctgtat gtataattcc tatatgtaat tttataggaa ttcttaactg
     7621 aaagtagtta tcaactgcta cttatcagga gcagtgttat tagggatttt aagttaggca
     7681 tgtagaaaac tttctgacaa aagggctatt gaactccagc cagttcccag tgaatctcca
     7741 ggaggcacct tagaagtctt cacaagctga ataccatgtc tttagggtgt ccaaatgctt
     7801 ctgtcttggg ggagagatga gagatgactg ggtgcctttc cactcagaac acagtagacc
     7861 actcactatt tatgaccaag cctcacttct tttctattac agttacagat tcttttttag
     7921 tctggaagtt agggtaaata aaatttgtat gtttgatttt tctcttagga atgttttctc
     7981 atcttaactc ttatgttatc tttatggcca acagatttgc tcagaatata tcatcaaaat
     8041 atatgtattg tattttgtaa aggtgaaagg atcgaaatac cactttgaca gttataaacc
     8101 agagtagatt tgagaactga ttgctcacat cacacttacg aatagtctca gaagccttta
     8161 agagaaactt acttctcaat ggacacttag aagaatttga tgatcttact gtcataaaat
     8221 cagtaatgaa attctcagca aaactatttg gtaaacattg caccctgtat ttttacccaa
     8281 catcttgtta tgaaaattat caaatacatt aaaaagttaa aataatttta tagtgtactc
     8341 actacctaga tgctaattag cattacctat gcttgctttg tcacatatcc attcatcatt
     8401 tcatattatt atttgatgca ttttaaattg catatcatta actagagttc attgttggct
     8461 tttttacata aaattagcat gcaatgaagt gcatagaaat atgtgttcac tgaggtttgg
     8521 caaatgcata tagtgctatg gaaataggaa acgttatcac caccctagaa agtttccaca
     8581 ctgccctttc cagtcaattc ctgtccctac ttgaccctca aaggcaacca ctgttccttt
     8641 ttctaccata agatttttct gttctaaaac tttatataaa tagagtcacg caacatgtac
     8701 gcttttctac aaggcttcat ttttttattg ctgttctttt ttattgctga gtagtattcc
     8761 actgtatgac aatattacag tttgttttct taatttccta ttattgatag atacctgggc
     8821 tgtttccagg attggccatt ataaataaag ctgctatgac cattcttacg taagtctttg
     8881 tgaacatagg ttttcatttc tttgcagtaa attccttgga ttagaattgc tgggtcacag
     8941 ggtagtttta aaagaaactg ccataccttt ttccagttca gttttattgt gccctgtatt
     9001 ttgtattttg atgtgcataa aggtgtaaac tatttttatg atccatattg aagtattaga
     9061 agaagtgagt ttggaataac aatggcattg ttcatggtca aggatgacaa ttggaactat
     9121 ctgaggaaag caaagacctg tctgccccag acagctcctc aaggggttcc tatgcctgaa
     9181 actttagcaa tatttcactc cttgaaaggt taaagcccaa ccatggtgat atgttagcta
     9241 gaagaaacag cactggtttg tttatgttac taactttcac cacccgtttt cagtaggtaa
     9301 agtaagactg atcattgaac agaaaaggtt atttctgtga tgaggttgag ggggtagtgg
     9361 aaagcatgta ttgctagtaa gccccaggta cagctatgtg agagagagtc acctggacag
     9421 ggaagtcccc caaatggtgg ccagaagagc agaaaacggg gtgggaaaaa ttctacctca
     9481 cagttttgca taggggatag ctatgctttt acgggacatt ttagcgttta aaaatatttt
     9541 caatattgtc tctttattca ataccaccca gctgtagttt tttcacactc tgatgactct
     9601 cttatccact cttttacctc tcccagctgt tctattacat acagcattca cctgtattag
     9661 tctatagaat tataatcatc tgtgtgtttc taatgctcat tttcaaaaat atgtatggtt
     9721 tattcgattt ttcctattaa aattttaagg gctgggaaat ttggcttcta ctgtaaatcc
     9781 ttataatgtt ccacacattg taaatatgca ataaaaatgt taacaactat aatggcagca
     9841 ggttttggta tgtatttctt tgatatattg tcactttgga caataagttt gatttctgtt
     9901 aataaaagtt gttcataaaa tctagggaga attaagtcac actggtctct ataagataat
     9961 ctgatgaatt ctgcatcttt gaatcaattt gagttatttt ttgtaaaggt ttaaattgtt
    10021 tctaacagtg ttacctaaaa cctttcatta aatcaaaaat gtaacttgta tattctgaaa
    10081 taacctggag tcttagaaga cagaggcttc tgcttccctc tcccgtgacc ccaacaatcc
    10141 cttcccacag gcttggcaca gaagttcagt gctttctcta cgtctcatgt tgtcagaaat
    10201 aggttctcac cataatagac agtggtgaaa tttccattgt tcctactgag aagtccattt
    10261 ttaaggcatc aaaggtgaat gtgtatttag ggatggagga ggcatattac aacaaagctg
    10321 tatttaaaat aacacaatca tatcatgcta taaacaatag cagttccaga atggctacta
    10381 aggattttgg actgccagac tttggaaatt ggcaagcctt ataccttgtc caatgaagtt
    10441 aagtacattt gagtgacagt tcaacataca agcaaaaaaa tatatagaag acattgtaca
    10501 tttgttatta atggacatgt ttactttaca tgttagagta tccatccatc tctaggtttt
    10561 ccattagatg cttcattgta tccacaaacc tgtttcaaac cattgaagtg atcttaccat
    10621 tgtgtttgtt caaaataaaa ttgggagaaa atgtgaatga gtcaatatca a
//
