LOCUS       NM_001271594            9279 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens sestrin 3 (SESN3), transcript variant 2, mRNA.
ACCESSION   NM_001271594
VERSION     NM_001271594.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9279)
  AUTHORS   Livneh,I., Fabre,B., Goldhirsh,G., Lulu,C., Zinger,A., Shammai
            Vainer,Y., Kaduri,M., Dahan,A., Ziv,T., Schroeder,A.,
            Ben-Neriah,Y., Zohar,Y., Cohen-Kaplan,V. and Ciechanover,A.
  TITLE     Inhibition of nucleo-cytoplasmic proteasome translocation by the
            aromatic amino acids or silencing Sestrin3-their sensing
            mediator-is tumor suppressive
  JOURNAL   Cell Death Differ 31 (10), 1242-1254 (2024)
   PUBMED   39266717
  REMARK    GeneRIF: Inhibition of nucleo-cytoplasmic proteasome translocation
            by the aromatic amino acids or silencing Sestrin3-their sensing
            mediator-is tumor suppressive.
REFERENCE   2  (bases 1 to 9279)
  AUTHORS   Luu,D., Shah,T., Sakharkar,P. and Min,D.I.
  TITLE     Genetic variations in a Sestrin2/Sestrin3/mTOR Axis and development
            of new-onset diabetes after kidney transplantation
  JOURNAL   Transpl Immunol 81, 101947 (2023)
   PUBMED   37918578
  REMARK    GeneRIF: Genetic variations in a Sestrin2/Sestrin3/mTOR Axis and
            development of new-onset diabetes after kidney transplantation.
REFERENCE   3  (bases 1 to 9279)
  AUTHORS   Chen,Y., Huang,T., Yu,Z., Yu,Q., Wang,Y., Hu,J., Shi,J. and Yang,G.
  TITLE     The functions and roles of sestrins in regulating human diseases
  JOURNAL   Cell Mol Biol Lett 27 (1), 2 (2022)
   PUBMED   34979914
  REMARK    GeneRIF: The functions and roles of sestrins in regulating human
            diseases.
            Review article
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 9279)
  AUTHORS   Zhang,L., Ren,R., Yang,X., Ge,Y., Zhang,X. and Yuan,H.
  TITLE     Oncogenic role of early growth response-1 in liver cancer through
            the regulation of the microRNA-675/sestrin 3 and the
            Wnt/beta-catenin signaling pathway
  JOURNAL   Bioengineered 12 (1), 5305-5322 (2021)
   PUBMED   34409922
  REMARK    GeneRIF: Oncogenic role of early growth response-1 in liver cancer
            through the regulation of the microRNA-675/sestrin 3 and the
            Wnt/beta-catenin signaling pathway.
REFERENCE   5  (bases 1 to 9279)
  AUTHORS   Waterhouse,M., Pennisi,S., Pfeifer,D., Deuter,M., von Bubnoff,N.,
            Scherer,F., Strussmann,T., Wehr,C., Duyster,J., Bertz,H., Finke,J.
            and Duque-Afonso,J.
  TITLE     Colon and liver tissue damage detection using methylated SESN3 and
            PTK2B genes in circulating cell-free DNA in patients with acute
            graft-versus-host disease
  JOURNAL   Bone Marrow Transplant 56 (2), 327-333 (2021)
   PUBMED   33082554
  REMARK    GeneRIF: Colon and liver tissue damage detection using methylated
            SESN3 and PTK2B genes in circulating cell-free DNA in patients with
            acute graft-versus-host disease.
            Erratum:[Bone Marrow Transplant. 2021 Oct;56(10):2616. doi:
            10.1038/s41409-021-01405-8.. PMID: 34413471]
REFERENCE   6  (bases 1 to 9279)
  AUTHORS   Hagenbuchner,J., Kuznetsov,A., Hermann,M., Hausott,B., Obexer,P.
            and Ausserlechner,M.J.
  TITLE     FOXO3-induced reactive oxygen species are regulated by BCL2L11
            (Bim) and SESN3
  JOURNAL   J Cell Sci 125 (Pt 5), 1191-1203 (2012)
   PUBMED   22349704
REFERENCE   7  (bases 1 to 9279)
  AUTHORS   Chen,C.C., Jeon,S.M., Bhaskar,P.T., Nogueira,V., Sundararajan,D.,
            Tonic,I., Park,Y. and Hay,N.
  TITLE     FoxOs inhibit mTORC1 and activate Akt by inducing the expression of
            Sestrin3 and Rictor
  JOURNAL   Dev Cell 18 (4), 592-604 (2010)
   PUBMED   20412774
  REMARK    GeneRIF: FoxOs inhibit mTORC1 and activate Akt by inducing the
            expression of Sestrin3 and Rictor
REFERENCE   8  (bases 1 to 9279)
  AUTHORS   Nogueira,V., Park,Y., Chen,C.C., Xu,P.Z., Chen,M.L., Tonic,I.,
            Unterman,T. and Hay,N.
  TITLE     Akt determines replicative senescence and oxidative or oncogenic
            premature senescence and sensitizes cells to oxidative apoptosis
  JOURNAL   Cancer Cell 14 (6), 458-470 (2008)
   PUBMED   19061837
REFERENCE   9  (bases 1 to 9279)
  AUTHORS   Kopnin,P.B., Agapova,L.S., Kopnin,B.P. and Chumakov,P.M.
  TITLE     Repression of sestrin family genes contributes to oncogenic
            Ras-induced reactive oxygen species up-regulation and genetic
            instability
  JOURNAL   Cancer Res 67 (10), 4671-4678 (2007)
   PUBMED   17510393
REFERENCE   10 (bases 1 to 9279)
  AUTHORS   Peeters,H., Debeer,P., Bairoch,A., Wilquet,V., Huysmans,C.,
            Parthoens,E., Fryns,J.P., Gewillig,M., Nakamura,Y., Niikawa,N., Van
            de Ven,W. and Devriendt,K.
  TITLE     PA26 is a candidate gene for heterotaxia in humans: identification
            of a novel PA26-related gene family in human and mouse
  JOURNAL   Hum Genet 112 (5-6), 573-580 (2003)
   PUBMED   12607115
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK302366.1 and AP000787.5.
            
            On Jun 1, 2019 this sequence version replaced NM_001271594.1.
            
            Summary: This gene encodes a member of the sestrin family of
            stress-induced proteins. The encoded protein reduces the levels of
            intracellular reactive oxygen species induced by activated Ras
            downstream of RAC-alpha serine/threonine-protein kinase (Akt) and
            FoxO transcription factor. The protein is required for normal
            regulation of blood glucose, insulin resistance and plays a role in
            lipid storage in obesity. Alternative splicing results in multiple
            transcript variants. [provided by RefSeq, Oct 2012].
            
            Transcript Variant: This variant (2) differs in the 5' UTR and uses
            an alternate in-frame splice site compared to variant 1. It encodes
            a shorter protein (isoform 2) compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK302366.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1644              AK302366.1         67-1710
            1645-9279           AP000787.5         46059-53693         c
FEATURES             Location/Qualifiers
     source          1..9279
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q21"
     gene            1..9279
                     /gene="SESN3"
                     /gene_synonym="SEST3"
                     /note="sestrin 3"
                     /db_xref="GeneID:143686"
                     /db_xref="HGNC:HGNC:23060"
                     /db_xref="MIM:607768"
     exon            1..319
                     /gene="SESN3"
                     /gene_synonym="SEST3"
                     /inference="alignment:Splign:2.1.0"
     exon            320..385
                     /gene="SESN3"
                     /gene_synonym="SEST3"
                     /inference="alignment:Splign:2.1.0"
     exon            386..583
                     /gene="SESN3"
                     /gene_synonym="SEST3"
                     /inference="alignment:Splign:2.1.0"
     CDS             476..1537
                     /gene="SESN3"
                     /gene_synonym="SEST3"
                     /note="isoform 2 is encoded by transcript variant 2"
                     /codon_start=1
                     /product="sestrin-3 isoform 2"
                     /protein_id="NP_001258523.1"
                     /db_xref="CCDS:CCDS60938.1"
                     /db_xref="GeneID:143686"
                     /db_xref="HGNC:HGNC:23060"
                     /db_xref="MIM:607768"
                     /translation="MSLHTQYLESFLRSQFYMLRMDGPLPLPYRHYIAIMKLVKTGEN
                     NWSLPELVHAVVLLAHYHALASFVFGSGINPERDPEISNGFRLISVNNFCVCDLANDN
                     NIENASLSGSNFGIVDSLSELEALMERMKRLQEEREDEEASQEEMSTRFEKEKKESLF
                     VVSGDTFHSFPHSDFEDDMIITSDVSRYIEDPGFGYEDFARRGEEHLPTFRAQDYTWE
                     NHGFSLVNRLYSDIGHLLDEKFRMVYNLTYNTMATHEDVDTTMLRRALFNYVHCMFGI
                     RYDDYDYGEVNQLLERSLKVYIKTVTCYPERTTKRMYDSYWRQFKHSEKVHVNLLLME
                     ARMQAELLYALRAITRHLT"
     exon            584..820
                     /gene="SESN3"
                     /gene_synonym="SEST3"
                     /inference="alignment:Splign:2.1.0"
     exon            821..995
                     /gene="SESN3"
                     /gene_synonym="SEST3"
                     /inference="alignment:Splign:2.1.0"
     exon            996..1114
                     /gene="SESN3"
                     /gene_synonym="SEST3"
                     /inference="alignment:Splign:2.1.0"
     exon            1115..1305
                     /gene="SESN3"
                     /gene_synonym="SEST3"
                     /inference="alignment:Splign:2.1.0"
     exon            1306..1450
                     /gene="SESN3"
                     /gene_synonym="SEST3"
                     /inference="alignment:Splign:2.1.0"
     exon            1451..9279
                     /gene="SESN3"
                     /gene_synonym="SEST3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9226..9231
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SESN3"
                     /gene_synonym="SEST3"
                     /note="hexamer: AATAAA"
     polyA_site      9252
                     /gene="SESN3"
                     /gene_synonym="SEST3"
                     /note="major polyA site"
ORIGIN      
        1 agtgagcaca gagctccttg ccagtaggcg atgcaagtta tctctggggc ccggaggaca
       61 cgagtgagga ccgggcacca atcaggttct cgctctgcgc cggcctttgt tctcactcgg
      121 gagcaggttg cgggcgtcta gcatcgggaa cccgcattcg actcggacgc gcattgctag
      181 ccccttcggg agacctggct cccctacccg tctgtcccgc cacactcggt cagcctgcgc
      241 tttccccagt caagtctgcc ttctctccgc agaacgtgga cgctaaaacc ctgacttacc
      301 attttaagct ttagaagagg ataaaagaat cagagtgtct caacccttga caagaggacc
      361 aagtgccttt attccagaga aggaagttgt ccaagcaaac acagtggatg aacgtactaa
      421 ctttcttgtg gaagaatact ctacatccgg tcgtctggac aacatcacac aggtcatgag
      481 tttacacact cagtacctgg agtctttctt gcggagccag ttttacatgt tgcgcatgga
      541 tggtcccctt cctctaccat acaggcacta tattgcaata atgaaacttg tcaaaactgg
      601 agaaaataat tggtctctgc ctgaactggt acatgctgtg gtcctcctgg cacattatca
      661 tgctttggca agctttgttt ttggtagtgg tatcaatcca gagagagatc cagaaatctc
      721 caatggattc aggctaatat cagtcaacaa tttctgcgtt tgtgatcttg ctaatgacaa
      781 caacatagag aatgcatctc tttcaggcag caactttggg attgtggatt ctctaagtga
      841 gctagaggcc ttaatggaaa ggatgaaaag acttcaagaa gaaagggaag atgaagaggc
      901 gtctcaagaa gaaatgagca ctcgttttga aaaggagaag aaagaaagtc tttttgtggt
      961 ctctggagat acttttcatt catttcctca ttcagatttt gaggatgaca tgattataac
     1021 atctgatgtc tctcgatata ttgaagaccc tggttttggg tatgaagact ttgccagacg
     1081 aggagaagag catttgccaa cattccgagc tcaggactat acctgggaaa atcatgggtt
     1141 ctccctggtg aacagacttt attctgacat tggacatctt cttgatgaaa agtttcggat
     1201 ggtctacaat ctcacatata acactatggc cacccatgag gatgttgaca caaccatgct
     1261 gcgcagagct ttatttaact atgttcactg tatgtttgga atcaggtatg atgactatga
     1321 ttatggagaa gttaatcaat tacttgaaag aagcctgaag gtttacatta agacagtgac
     1381 ctgctatcct gagagaacta caaaacgcat gtatgatagt tactggcggc agttcaaaca
     1441 ctcagaaaaa gttcatgtca atctactttt aatggaagca cgaatgcaag ctgaacttct
     1501 ttatgctctt cgtgccataa ctcggcattt gacctgaagt atcacccaag gaaaatgtca
     1561 tacatgtgta aagacctttc acataagata aacattgaag ctatttgtga tatagcatca
     1621 aaaattattc agttctctag tgtcaaagtt tagccgtttg tttttttttt tttgtttttt
     1681 gcggctgtaa tgtgcaatga tgtgttttat tttccttgat gcttaacatt actaacaatt
     1741 gcaaaaataa tactgaggag cactactttg cattgtttgt agttggagtt ttggatactg
     1801 atcataaatc atgaatctgg cgtattaatg cttaacttca gcgcctttgg gttgtgtgta
     1861 tgtgtatttt ttctcccccc accccgccct ttttttttta agaataagga caagtgctta
     1921 caatctgctc aagcaattgt ttttcagata ttggaattca gcaaagagtg acctctctgg
     1981 ccattctaaa actttaatgt tgtgtcctaa atctggtgga ggaaaagcat gcgctgttga
     2041 accaagctgc tcactggaca catatttgtg tccccaggag attatttgtc aggcactgtt
     2101 ttttatttgt ttgtttgttc atttattgtt gttttactgc tattggcgat aacatgaaat
     2161 gtgatacagc tattgcccat aatttaatgt gaaattaatc atgatgaatt ctatagcatg
     2221 ctactgaaat gccaaattca gctattttct ttctcttcta aaatggaagt ttcagttttc
     2281 catatcagat gtatataagt ggaattataa cacaatccta tcactgacag taatcctcag
     2341 tcaccttaaa taatcagtgc tcacagtgct gcttacagtt catttagttg gcacatataa
     2401 acctgtaccc acacaagact gaccaaaaat tttctttgca catttttcct ttctcatagt
     2461 ttttttctat atttcgttaa gcaaaggaag gttaatatgc ctatttttcc tgttaggctt
     2521 ttagaaatta ttttccatga cttttgttct tataataggt tagacagttt atttttcaaa
     2581 ggtaaaaatt gtgtttctat gcattaatgt gattgaacta caaaaacaga gtgcaatatg
     2641 ttcaaaaaat actcaactaa actttccccc tgtcatatca ctgcaaaagt gtccttttaa
     2701 acagagccat aaagaaattt ttttcaaatt tctttattgc acaggctttt ttcagtttta
     2761 cctttctggg attgaacaat gttcctttca taatgccaaa gcattccctt ccccagaaaa
     2821 atctccttgg acaactggtt taaaaacgtt attaagtcat aggggaggaa taagcggcca
     2881 aaaagtcaca tttttgcatt ggtttacgtt gcttgattag actatcttca gagagcacat
     2941 tatgtgttta tagctttaat ttgtattatg tcaatgaacc agtgaagtgc ctaaagagat
     3001 gcttacaact atccagtagg acacaactgc actgcttttt aaacactttt tggttagtta
     3061 agggtgactt gaattgtaca catacatgct aatttttgaa aacttatcca tttttcatag
     3121 taaataatat agataaaatt ttctgttaga gttcccgcaa atatttcatt ttagacttac
     3181 ttgtgatata agatttaact tttcttttgt gaaagaatcg tgaactagtg tttcatgaaa
     3241 tcattttcct cttccattta tcatcctcct ctggtacaat atattgggtt gctttctttt
     3301 agattttgtt ccagatagga aaacaaatat gactgtatac ttattaacat aaggtagttc
     3361 tgcatttttg gattccagga tcctgtttga aatcttccat gtgaggaaaa aattactaat
     3421 atttttaaag acagggtggt ttatttaaag agattattca gagaacttgt gccatatctc
     3481 gtctgtttta ttgcatatgc catatgttaa cttttattca atattacatt atgtagatat
     3541 gtaatacaaa gaaaatattt aggagaatgg caaaacacaa atggcaacat aaatgtccat
     3601 ttgacttacc taacttcaca actttcaagt tgaggatgtc atttattctt gaatttgttt
     3661 ttttactaga tgctttcaat taatagccct atatttttgt gcaggcgaac tgtataacag
     3721 gataaaaaat gatttgtatg tattgaaaag gaggagaaat tctcacagaa caccatatga
     3781 gctttagacc aaaaggggaa acaaggttta agtaactaaa atggccactt agtttgtgtt
     3841 tatttttttc cctggaaatg taagtagttg gagtttaggc tatggaaata ttagtgcctt
     3901 taatagatct ctttccttgc aaagtttctt tttagctcag cattgatcta tcttatcaat
     3961 aggaaaataa gttgacttgg aactataaac aaaaacaaca ccatatattt atgaacctca
     4021 gtgaaccagc ctagaaaaat tgacttctgc aggtgctaaa gcacctacta atacgcagca
     4081 tgcacagcat ttcaggttgt gaataccatt ttacaacgag tttgctgtta actctcttta
     4141 tccaatgcaa acttgaaatt ctgatgcggt tttccagggt ggtatttttt cagagtattg
     4201 aatttactat ttagtaattt atagtatagc tttttatatt aaaatgtgat atttttaaaa
     4261 gagatatctg gttcaattgg tataatgacg tgattatgca atatgctgat ctacaccttg
     4321 cgctcacatt gtgccaaact taaatgtgca agtgtacgtg agaaaagcac atgtgaatgt
     4381 gaattcttta attctttgct tattatgtta aaattagtct tcatagtcca ctgatgtgta
     4441 tgcttgaata atgtctattt actttggaat ttcaagattt ccagcttcaa tggtaattta
     4501 taatttctca gatcacctta agaaatattg agatactctc ccacctgaaa ataatctgtt
     4561 ctttaaccca cctgactatg ggagtagcca ggcaggtgcc tcactggctt cttcagacga
     4621 ggcttcctca ggtggatata tgactattag gggaaaagag gcttttgaga cagcttatta
     4681 cctccttccc ctttttgaac taagtacatt gtctcagtcc cagtatgaat ttgtcccatt
     4741 tggggtattt ttaaaataaa agatggctaa catggaatct ggggcatagc cttggccttt
     4801 aattatggtt gtaagcccaa ataatggcag agaaacacag ggctgttttt acaacagaaa
     4861 actcaatata agttttatat aagaatccta taaaatttga accagagctc ctatttagtt
     4921 gttataatgt ctcctaaaga tcttaaatgc ttctaaaagc taattattat tccagagcaa
     4981 ctttttcctt ttccccctta taaagttttt ttttaaatcc aaaatcttag atttctgtgc
     5041 cacagcaaaa taaaatgatc aacaattaaa gatctgtact taaaatagta caccattttt
     5101 agtggaggga gggtgcatgc ctatatgatg gtaagattct tttgaactct aaggacaatt
     5161 cctcatggaa aaaataaatt tcagtgcata ttataaaaat gatttgcact gtgttagcaa
     5221 aataaggtgt tttaggttgt attttaaaga caattctaaa tcatttctct ggaaagcaag
     5281 accttaaatt cttctcttta atagaactgc ttttatgcaa agagatggtc ttaacatgga
     5341 ctaaacagta cataacaata atgtttgtta tgtaaatagt atatcacatt tgcttagtag
     5401 cacaattcaa cagcaggctg aaagtcgtgg ctcctctgct cagattgagt tttaagttac
     5461 ctaatttatg taatcagaat ctattctagg tggccgtcaa cgtaaactgt gtttccctat
     5521 tatatccctc tggagactga gaatcaaatt aaatagtctt tcttcaaagc agtactgtaa
     5581 catgaatgta tttatctcag tcaacagaac ttatatgccc actgtatgtg gagcttattt
     5641 caatgctaat ttggcttagt tagcagacct agaatctgcc caggtgagac ctagaacaaa
     5701 aatagctggg gtgaaatgga taagagaggt agaggtatat gtcaaggcag agccctatga
     5761 ggaaggaaga gttttcaaag aatatgagga acatagtgct gagagtgtgg ctgccttcag
     5821 caccgtacac ctaatctaga gaaaatattt cccatgtggg aggtcctgtc tgcattcagt
     5881 ccaccctttt ctgcctgctt cttcctccaa gtgcctcaac ctctacatgc tcactctcct
     5941 ccccttccct cagcccatct tggtctaagc agctttcaca atccaaacca aacatcacca
     6001 gccacccgct gataagtcac cagcatttac tttcctgagt tactttttct ccattcattg
     6061 agactatgga ttcatcccaa ctccttctaa atccctcaac catccagcta tattttggct
     6121 aacctttgcc ctagacactc taccagatgt taatgcagta tcaagtgtaa attgtgtcac
     6181 cctattctgt tctacccttt tccctgctgc cgaaatatct tgctctcctc tacctcatcc
     6241 ccaaagagcc tataaattca gagtatccaa ccttttcatg gattcactca ctgttgttca
     6301 gtaatacaac ttccatattt taaataaatc ataaaagatt tttgccttct atcaaagtag
     6361 gaaactttat atttatacat gatacagaaa ttgaactatt tctatcgttc agaatttagc
     6421 atataacagg attttaaagt gattgaattg ccatcctaac ttggatttgt attaggttat
     6481 gaatgaatac tgttgttagt ccaagctatt tgaatgtaaa gttaaaattc tccagcaatc
     6541 cagaatgact tgtaaccctt cagtggaatg ggaaatttct ggtatgcaaa tagggtggtg
     6601 ttccagacat ttgttttatt ggtggtgttt gggccttgaa acacatttct aatagaatga
     6661 catatggcaa agggtattta gatggtccta tgggatcaca gaagcccata tgatttaaaa
     6721 tatagataaa ataacacaaa attgggtaca taagggttta accaggcttt cctgggttag
     6781 actaagcctc taacctctgt tcctatcctg ttcttatggg aggaaaaaaa atgttccatg
     6841 ggatgaaaag aaaatgcatt gactttgtag tctttattct cctagccgca cttcgggtat
     6901 atccagaagg aagaggaaat gcaaagagga agaagatgta atagttcttc tctccagcta
     6961 ggtgcacttg aggttgttca taaatgtaaa attatgtcag gtttctaaca tgggacactg
     7021 cacacagttg tctgacctga tgaaccatcc catttgaaag tatagattat tattatttct
     7081 tgtagtattt ggttgttttc catctcattc atgaacaact caacctgata gtagtatcca
     7141 ataaatgcct ttcagggctc aggaatgaat tgacatccta gttaagaaat gagacttaat
     7201 aatggagact gaatgaggcg gtttgtatta aattatatgc catgaagtgt tcattttagc
     7261 tttaacctaa ttatgactgt accaccatga agtacagaat gaaaaattat atatatgggg
     7321 gggaaacaga atgaatatct gattcttttg aatgcttgtg gaaatctttg agatcgtgca
     7381 gggcatacca caaaatagcc tttagaacag atacccaatt ttacagttca taggacaaca
     7441 tcaaacatta gtaagtctaa ataagatgaa tagaattttt gttatgtaaa ttttgctaga
     7501 acagtctatt ttcttgcacc cctcaagtta acctcttaaa aaaatgaatg tataatttct
     7561 accgaaagaa tatcagagag aatctctctg gcctatagtg ttaaaatatt gttcacaaat
     7621 cctgattagt taagtgcata cattatgaaa cttacagaat aaaacttatt atacatctct
     7681 ttcttaaatt aatatcttta cacattttca actggctccc caagtctgat aaggaaggat
     7741 taaaagaaaa aagaaatgta ttagttgggt ggccaaggag tttcctttgt aatgttgaga
     7801 gacttccgct ttctgaattt cgctggttct ctaaggtaaa agagttaaat agtacccttg
     7861 ttcaccaagg aaagtgatcc aaactatata tctagtgcag atatttcctt tgcattattt
     7921 agtcttctct ggagagaaaa tacagtttcc ccttcctctt tctcttcaca tttactcttt
     7981 tcaacccaaa ataagagaca tagaaagcaa accacagcca gtttggcatc ttctcagtgc
     8041 tactagtata ggcacataca catacacagt ctcagcaagg ttataaagaa ccctgtcagg
     8101 tccacttgca acatggcctt gctacttgga ttagctcctt taagcctgaa aataactttc
     8161 ctggtcatgg aagaactgga cgcatctttt aacttatgaa atagaagttg aacttgaaaa
     8221 ctctttttaa aaaatcctgg ttttgcagga cagctacata atgaatgtat atattaagac
     8281 tgtagctgaa ttgcacatga aatcagattg ccaacttctt gactttcaat gttagacatt
     8341 tatccttaag ttgtgagcga tatatgtagc atgctgtgaa atgtctgtta tagctcttta
     8401 attcatcagt attaatacag aattatcatt tgcgtttctt ggtacttttt attcaatgta
     8461 atcagaagct gtgatgtttt gcctttgtag tcctgtgctt tgttactgta attttttttt
     8521 tttttttacg aagcacgtga ctggactaat gtaaggcaga tgacgtgatc tttaagactg
     8581 ctatatatat cagtctctta ctctataagg ttttaaatta gaataagctt ttatcaaata
     8641 gataattgat gcaatttagg attcacgcaa gtttcagtgt caaatggcgg tcttatagtt
     8701 tcaattctga aaatagcaaa cttaataaac agccacttta aacttgttct ggcaaaccag
     8761 accctgctgt agatatagtc taaggtagtt aaccatataa gccttttcaa ctcttaatgc
     8821 cctccacatg aatcagcagt taagaaggtt ctagaaccca tgaaagcttt tgtatgtatt
     8881 actaggtttt gtttttctta tgtttgctga ttttacagtt ctgactaaag ctgacctaaa
     8941 tggatcagtt tatgtgtaat attctagtgc tttaatgact ctttttttct ttggagggag
     9001 ggtaacatta tttggacaga tgcagaagga actgttagtg agtcaagaca aacacatctg
     9061 aaataaagga actgtgtatt aacatgttaa caattcataa ctgcactttt tatgacattt
     9121 tgaaaatcta tttataggta cagaacaatg ggttttgtta aactgtatca catttatact
     9181 tgcagaaatt tatttcattg ttattagtag gaattttatt ggttcaataa aattggcaaa
     9241 actgaacacc aatcatttgc ctactttgtt tatactgga
//
