LOCUS       NM_000934               2249 bp    mRNA    linear   PRI 08-MAY-2024
DEFINITION  Homo sapiens serpin family F member 2 (SERPINF2), transcript
            variant 1, mRNA.
ACCESSION   NM_000934
VERSION     NM_000934.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Pechlivani,N., Kearney,K.J. and Ajjan,R.A.
  TITLE     Fibrinogen and Antifibrinolytic Proteins: Interactions and Future
            Therapeutics
  JOURNAL   Int J Mol Sci 22 (22), 12537 (2021)
   PUBMED   34830419
  REMARK    GeneRIF: Fibrinogen and Antifibrinolytic Proteins: Interactions and
            Future Therapeutics.
            Review article
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Niwa,H., Kanno,Y., Shu,E. and Seishima,M.
  TITLE     Decrease in matrix metalloproteinase-3 activity in systemic
            sclerosis fibroblasts causes alpha2-antiplasmin and extracellular
            matrix deposition, and contributes to fibrosis development
  JOURNAL   Mol Med Rep 22 (4), 3001-3007 (2020)
   PUBMED   32945390
  REMARK    GeneRIF: Decrease in matrix metalloproteinase3 activity in systemic
            sclerosis fibroblasts causes alpha2antiplasmin and extracellular
            matrix deposition, and contributes to fibrosis development.
REFERENCE   3  (bases 1 to 2249)
  AUTHORS   Bryk,A.H., Satala,D., Natorska,J., Rapala-Kozik,M. and Undas,A.
  TITLE     Interaction of glycated and acetylated human alpha2-antiplasmin
            with fibrin clots
  JOURNAL   Blood Coagul Fibrinolysis 31 (6), 393-396 (2020)
   PUBMED   32815915
  REMARK    GeneRIF: Interaction of glycated and acetylated human
            alpha2-antiplasmin with fibrin clots.
REFERENCE   4  (bases 1 to 2249)
  AUTHORS   Bryk,A.H., Cysewski,D., Dadlez,M. and Undas,A.
  TITLE     Identification of glycated and acetylated lysine residues in human
            alpha2-antiplasmin
  JOURNAL   Biochem Biophys Res Commun 521 (1), 19-23 (2020)
   PUBMED   31653347
  REMARK    GeneRIF: human alpha2-antiplasmin is glycated and acetylated at
            several sites, with the possible competition between acetylation
            and glycation at K-182 and K-448; finding suggests possibly
            relevant alterations to alpha2-antiplasmin function at high
            glycemia and during aspirin use
REFERENCE   5  (bases 1 to 2249)
  AUTHORS   Bangert,K., Johnsen,A.H., Christensen,U. and Thorsen,S.
  TITLE     Different N-terminal forms of alpha 2-plasmin inhibitor in human
            plasma
  JOURNAL   Biochem J 291 (Pt 2) (Pt 2), 623-625 (1993)
   PUBMED   8484741
REFERENCE   6  (bases 1 to 2249)
  AUTHORS   Hirosawa,S., Nakamura,Y., Miura,O., Sumi,Y. and Aoki,N.
  TITLE     Organization of the human alpha 2-plasmin inhibitor gene
  JOURNAL   Proc Natl Acad Sci U S A 85 (18), 6836-6840 (1988)
   PUBMED   3166140
  REMARK    Erratum:[Proc Natl Acad Sci U S A 1989 Mar;86(5):1612-13]
REFERENCE   7  (bases 1 to 2249)
  AUTHORS   Lijnen,H.R., Holmes,W.E., van Hoef,B., Wiman,B., Rodriguez,H. and
            Collen,D.
  TITLE     Amino-acid sequence of human alpha 2-antiplasmin
  JOURNAL   Eur J Biochem 166 (3), 565-574 (1987)
   PUBMED   2440681
REFERENCE   8  (bases 1 to 2249)
  AUTHORS   Wiman,B. and Collen,D.
  TITLE     On the mechanism of the reaction between human alpha 2-antiplasmin
            and plasmin
  JOURNAL   J Biol Chem 254 (18), 9291-9297 (1979)
   PUBMED   158022
REFERENCE   9  (bases 1 to 2249)
  AUTHORS   Wiman,B. and Collen,D.
  TITLE     Purification and characterization of human antiplasmin, the
            fast-acting plasmin inhibitor in plasma
  JOURNAL   Eur J Biochem 78 (1), 19-26 (1977)
   PUBMED   21075
REFERENCE   10 (bases 1 to 2249)
  AUTHORS   Moroi,M. and Aoki,N.
  TITLE     Isolation and characterization of alpha2-plasmin inhibitor from
            human plasma. A novel proteinase inhibitor which inhibits
            activator-induced clot lysis
  JOURNAL   J Biol Chem 251 (19), 5956-5965 (1976)
   PUBMED   134998
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BX107460.1, BC031592.1 and
            J02654.1.
            
            On Dec 27, 2019 this sequence version replaced NM_000934.3.
            
            Summary: This gene encodes a member of the serpin family of serine
            protease inhibitors. The protein is a major inhibitor of plasmin,
            which degrades fibrin and various other proteins. Consequently, the
            proper function of this gene has a major role in regulating the
            blood clotting pathway. Mutations in this gene result in
            alpha-2-plasmin inhibitor deficiency, which is characterized by
            severe hemorrhagic diathesis. Multiple transcript variants encoding
            different isoforms have been found for this gene. [provided by
            RefSeq, Sep 2009].
            
            Transcript Variant: This variant (1) represents the longest
            transcript. Both variants 1 and 2 encode the same isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038197.1206999.1,
                                           SRR14038191.2900383.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968968
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000453066.6/ ENSP00000402286.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-702               BX107460.1         36-737
            703-1588            BC031592.1         716-1601
            1589-1599           J02654.1           1538-1548
            1600-1664           J02654.1           1550-1614
            1665-1678           BC031592.1         1678-1691
            1679-2249           J02654.1           1628-2198
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17p13.3"
     gene            1..2249
                     /gene="SERPINF2"
                     /gene_synonym="A2AP; AAP; ALPHA-2-PI; alpha2AP; API; PLI"
                     /note="serpin family F member 2"
                     /db_xref="GeneID:5345"
                     /db_xref="HGNC:HGNC:9075"
                     /db_xref="MIM:613168"
     exon            1..38
                     /gene="SERPINF2"
                     /gene_synonym="A2AP; AAP; ALPHA-2-PI; alpha2AP; API; PLI"
                     /inference="alignment:Splign:2.1.0"
     exon            39..105
                     /gene="SERPINF2"
                     /gene_synonym="A2AP; AAP; ALPHA-2-PI; alpha2AP; API; PLI"
                     /inference="alignment:Splign:2.1.0"
     CDS             43..1518
                     /gene="SERPINF2"
                     /gene_synonym="A2AP; AAP; ALPHA-2-PI; alpha2AP; API; PLI"
                     /note="isoform a precursor is encoded by transcript
                     variant 1; alpha-2-plasmin inhibitor; serine (or cysteine)
                     proteinase inhibitor, clade F (alpha-2 antiplasmin,
                     pigment epithelium derived factor), member 2; serpin F2;
                     alpha-2-AP; serpin peptidase inhibitor, clade F (alpha-2
                     antiplasmin, pigment epithelium derived factor), member 2"
                     /codon_start=1
                     /product="alpha-2-antiplasmin isoform a precursor"
                     /protein_id="NP_000925.2"
                     /db_xref="CCDS:CCDS11011.1"
                     /db_xref="GeneID:5345"
                     /db_xref="HGNC:HGNC:9075"
                     /db_xref="MIM:613168"
                     /translation="MALLWGLLVLSWSCLQGPCSVFSPVSAMEPLGRQLTSGPNQEQV
                     SPLTLLKLGNQEPGGQTALKSPPGVCSRDPTPEQTHRLARAMMAFTADLFSLVAQTST
                     CPNLILSPLSVALALSHLALGAQNHTLQRLQQVLHAGSGPCLPHLLSRLCQDLGPGAF
                     RLAARMYLQKGFPIKEDFLEQSEQLFGAKPVSLTGKQEDDLANINQWVKEATEGKIQE
                     FLSGLPEDTVLLLLNAIHFQGFWRNKFDPSLTQRDSFHLDEQFTVPVEMMQARTYPLR
                     WFLLEQPEIQVAHFPFKNNMSFVVLVPTHFEWNVSQVLANLSWDTLHPPLVWERPTKV
                     RLPKLYLKHQMDLVATLSQLGLQELFQAPDLRGISEQSLVVSGVQHQSTLELSEVGVE
                     AAAATSIAMSRMSLSSFSVNRPFLFFIFEDTTGLPLFVGSVRNPNPSAPRELKEQQDS
                     PGNKDFLQSLKGFPRGDKLFGPDLKLVPPMEEDYPQFGSPK"
     exon            106..144
                     /gene="SERPINF2"
                     /gene_synonym="A2AP; AAP; ALPHA-2-PI; alpha2AP; API; PLI"
                     /inference="alignment:Splign:2.1.0"
     exon            145..207
                     /gene="SERPINF2"
                     /gene_synonym="A2AP; AAP; ALPHA-2-PI; alpha2AP; API; PLI"
                     /inference="alignment:Splign:2.1.0"
     exon            208..409
                     /gene="SERPINF2"
                     /gene_synonym="A2AP; AAP; ALPHA-2-PI; alpha2AP; API; PLI"
                     /inference="alignment:Splign:2.1.0"
     exon            410..553
                     /gene="SERPINF2"
                     /gene_synonym="A2AP; AAP; ALPHA-2-PI; alpha2AP; API; PLI"
                     /inference="alignment:Splign:2.1.0"
     exon            554..757
                     /gene="SERPINF2"
                     /gene_synonym="A2AP; AAP; ALPHA-2-PI; alpha2AP; API; PLI"
                     /inference="alignment:Splign:2.1.0"
     exon            758..900
                     /gene="SERPINF2"
                     /gene_synonym="A2AP; AAP; ALPHA-2-PI; alpha2AP; API; PLI"
                     /inference="alignment:Splign:2.1.0"
     exon            901..1105
                     /gene="SERPINF2"
                     /gene_synonym="A2AP; AAP; ALPHA-2-PI; alpha2AP; API; PLI"
                     /inference="alignment:Splign:2.1.0"
     exon            1106..2249
                     /gene="SERPINF2"
                     /gene_synonym="A2AP; AAP; ALPHA-2-PI; alpha2AP; API; PLI"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2232..2237
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SERPINF2"
                     /gene_synonym="A2AP; AAP; ALPHA-2-PI; alpha2AP; API; PLI"
                     /note="hexamer: AATAAA"
     polyA_site      2249
                     /gene="SERPINF2"
                     /gene_synonym="A2AP; AAP; ALPHA-2-PI; alpha2AP; API; PLI"
                     /note="major polyA site"
ORIGIN      
        1 agaggaacgt tgtgtgtggc agcaaggagc ccgcagagga acatggcgct gctctggggg
       61 ctcctggtgc tcagctggtc ctgcctgcaa ggcccctgct ccgtgttctc ccctgtgagc
      121 gccatggagc ccttgggccg gcagctaact agcgggccga accaggagca ggtgtcccca
      181 cttaccctcc tcaagttggg caaccaggag cctggtggcc agactgccct gaagagtccc
      241 ccaggagtct gcagcagaga ccccacccca gagcagaccc acaggctggc ccgggccatg
      301 atggccttca ctgccgacct gttctccctg gtggctcaaa cgtccacctg ccccaacctc
      361 atcctgtcac ccctgagtgt ggccctggcg ctgtctcacc tggcactagg tgctcagaac
      421 cacacgttgc agaggctgca acaggtgctg cacgcaggct cagggccctg cctcccccat
      481 ctgctgagcc gcctctgcca ggacctgggc cccggcgcgt tccgactggc tgccaggatg
      541 tacctgcaga aaggatttcc catcaaagaa gatttcctgg aacaatccga acagctattt
      601 ggggcaaagc ccgtgagcct gacgggaaag caggaagatg acctggcaaa catcaaccaa
      661 tgggtgaagg aggccacgga ggggaagatt caggaattcc tctctgggct gccggaagac
      721 accgtgttgc ttctcctcaa cgccatccac ttccagggtt tctggaggaa caagtttgac
      781 ccgagcctta cccagagaga ctccttccac ctggacgagc agttcacggt gcccgtggaa
      841 atgatgcagg cccgcacgta cccgctgcgc tggttcttgc tggagcagcc tgagatccag
      901 gtggctcatt tcccctttaa gaacaacatg agctttgtgg tccttgtacc cacccacttt
      961 gaatggaacg tgtcccaggt actggccaac ctgagttggg acaccctgca cccacctctg
     1021 gtgtgggaga ggcccaccaa ggtccggctg cctaagctgt atctgaaaca ccaaatggac
     1081 ctggtggcca ccctcagcca gctgggcctg caggagttgt tccaggcccc agacctgcgt
     1141 gggatctccg agcagagcct ggtggtgtcc ggcgtgcagc atcagtccac cctggagctc
     1201 agcgaggtcg gcgtggaggc ggcggcggcc accagcattg ccatgtcccg catgtccctg
     1261 tcctccttca gcgtgaaccg ccccttcctc ttcttcatct tcgaggacac cacaggcctt
     1321 cccctcttcg tgggcagcgt gaggaacccc aaccccagtg caccgcggga gctcaaggaa
     1381 cagcaggatt ccccgggcaa caaggacttc ctccagagcc tgaaaggctt cccccgcgga
     1441 gacaagcttt tcggccctga cttaaaactt gtgcccccca tggaggagga ttacccccag
     1501 tttggcagcc ccaagtgagg ggccgtggct gtggcatcca gagtccctgc ctggaccagc
     1561 ctctccactc atgtgactct ttccaaccgg ctttgtggca ctggggcagg ggccgggggc
     1621 agtctgagag aggccattct ttcccaacac ctcttgggga gtttagggtg gggggggggc
     1681 gcggctggga ggagggcagg catcggggag ccgggagcct gaccctcatc tttcttccaa
     1741 acaggctcag agggtgtcct gcaccggggc ctgggcagga gggaggtgct tctagttctg
     1801 ccaggagaca ggttagctgc tccccacgtc agctgggaca ccccgacttt tgtttaccag
     1861 agaaaaaggg agggggagag ggctgccttt ggacttgtcc cgggacacct aggctagggt
     1921 ggggagagac gggccctggt ggtggctcgg gaggcgaagc gttgtcctca gccccgcgtg
     1981 gaactcgtgt ctggcacagc ctggctgtgg cctaacctgc cgagagtcca tcagcctcca
     2041 tcctaccccc tgtgccttgt cacgccagac ttcccacggc tcctcgagat cccaacactg
     2101 ccagcatttc ccttccttcc tctcctgtct ccctcctctg cccgggagct caggaaccga
     2161 ggcagggaag gatcccatga gctccttaag gctcttttgt aaggtttttg tagtgatttt
     2221 tatgccacct gaataaagaa tgaatgggc
//
