LOCUS       NM_001136528            2226 bp    mRNA    linear   PRI 09-JUL-2024
DEFINITION  Homo sapiens serpin family E member 2 (SERPINE2), transcript
            variant 2, mRNA.
ACCESSION   NM_001136528
VERSION     NM_001136528.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Zhang,S., Jia,X., Dai,H., Zhu,X., Song,W., Bian,S., Wu,H., Chen,S.,
            Tang,Y., Chen,J., Jin,C., Zhou,M., Xie,H., Zheng,S. and Song,P.
  TITLE     SERPINE2 promotes liver cancer metastasis by inhibiting
            c-Cbl-mediated EGFR ubiquitination and degradation
  JOURNAL   Cancer Commun (Lond) 44 (3), 384-407 (2024)
   PUBMED   38407942
  REMARK    GeneRIF: SERPINE2 promotes liver cancer metastasis by inhibiting
            c-Cbl-mediated EGFR ubiquitination and degradation.
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Qu,H., Jiang,J., Zhan,X., Liang,Y., Guo,Q., Liu,P., Lu,L., Yang,Y.,
            Xu,W., Zhang,Y., Lan,S., Chen,Z., Lu,Y., Ou,Y. and Qin,Y.
  TITLE     Integrating artificial intelligence in osteosarcoma prognosis: the
            prognostic significance of SERPINE2 and CPT1B biomarkers
  JOURNAL   Sci Rep 14 (1), 4318 (2024)
   PUBMED   38383657
  REMARK    GeneRIF: Integrating artificial intelligence in osteosarcoma
            prognosis: the prognostic significance of SERPINE2 and CPT1B
            biomarkers.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2226)
  AUTHORS   Zhang,D., Sun,R., Di,C., Li,L., Zhao,F., Han,Y. and Zhang,W.
  TITLE     Microdissection of cancer-associated fibroblast infiltration
            subtypes unveils the secreted SERPINE2 contributing to
            immunosuppressive microenvironment and immuotherapeutic resistance
            in gastric cancer: A large-scale study integrating bulk and
            single-cell transcriptome profiling
  JOURNAL   Comput Biol Med 166, 107406 (2023)
   PUBMED   37729702
  REMARK    GeneRIF: Microdissection of cancer-associated fibroblast
            infiltration subtypes unveils the secreted SERPINE2 contributing to
            immunosuppressive microenvironment and immuotherapeutic resistance
            in gastric cancer: A large-scale study integrating bulk and
            single-cell transcriptome profiling.
REFERENCE   4  (bases 1 to 2226)
  AUTHORS   Zhou,B., Mo,Z., Lai,G., Chen,X., Li,R., Wu,R., Zhu,J. and Zheng,F.
  TITLE     Targeting tumor exosomal circular RNA cSERPINE2 suppresses breast
            cancer progression by modulating MALT1-NF-B-IL-6 axis of
            tumor-associated macrophages
  JOURNAL   J Exp Clin Cancer Res 42 (1), 48 (2023)
   PUBMED   36797769
  REMARK    GeneRIF: Targeting tumor exosomal circular RNA cSERPINE2 suppresses
            breast cancer progression by modulating MALT1-NF-B-IL-6 axis of
            tumor-associated macrophages.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2226)
  AUTHORS   Naldini,L., Tamagnone,L., Vigna,E., Sachs,M., Hartmann,G.,
            Birchmeier,W., Daikuhara,Y., Tsubouchi,H., Blasi,F. and
            Comoglio,P.M.
  TITLE     Extracellular proteolytic cleavage by urokinase is required for
            activation of hepatocyte growth factor/scatter factor
  JOURNAL   EMBO J 11 (13), 4825-4833 (1992)
   PUBMED   1334458
REFERENCE   6  (bases 1 to 2226)
  AUTHORS   Evans,D.L., McGrogan,M., Scott,R.W. and Carrell,R.W.
  TITLE     Protease specificity and heparin binding and activation of
            recombinant protease nexin I
  JOURNAL   J Biol Chem 266 (33), 22307-22312 (1991)
   PUBMED   1939253
REFERENCE   7  (bases 1 to 2226)
  AUTHORS   Cavanaugh,K.P., Gurwitz,D., Cunningham,D.D. and Bradshaw,R.A.
  TITLE     Reciprocal modulation of astrocyte stellation by thrombin and
            protease nexin-1
  JOURNAL   J Neurochem 54 (5), 1735-1743 (1990)
   PUBMED   1691280
REFERENCE   8  (bases 1 to 2226)
  AUTHORS   Sommer,J., Gloor,S.M., Rovelli,G.F., Hofsteenge,J., Nick,H.,
            Meier,R. and Monard,D.
  TITLE     cDNA sequence coding for a rat glia-derived nexin and its homology
            to members of the serpin superfamily
  JOURNAL   Biochemistry 26 (20), 6407-6410 (1987)
   PUBMED   3427015
REFERENCE   9  (bases 1 to 2226)
  AUTHORS   Gloor,S., Odink,K., Guenther,J., Nick,H. and Monard,D.
  TITLE     A glia-derived neurite promoting factor with protease inhibitory
            activity belongs to the protease nexins
  JOURNAL   Cell 47 (5), 687-693 (1986)
   PUBMED   2877744
REFERENCE   10 (bases 1 to 2226)
  AUTHORS   Scott,R.W., Bergman,B.L., Bajpai,A., Hersh,R.T., Rodriguez,H.,
            Jones,B.N., Barreda,C., Watts,S. and Baker,J.B.
  TITLE     Protease nexin. Properties and a modified purification procedure
  JOURNAL   J Biol Chem 260 (11), 7029-7034 (1985)
   PUBMED   3997857
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK225823.1, AC073641.3,
            AI754402.1 and DB325994.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Aug 7, 2019 this sequence version replaced NM_001136528.1.
            
            Summary: This gene encodes a member of the serpin family of
            proteins, a group of proteins that inhibit serine proteases.
            Thrombin, urokinase, plasmin and trypsin are among the proteases
            that this family member can inhibit. This gene is a susceptibility
            gene for chronic obstructive pulmonary disease and for emphysema.
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Aug 2012].
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site in the 3' coding region, compared to variant 1,
            resulting in an isoform (b) that is 1 aa shorter than isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038197.2408345.1, AK225823.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2142853, SAMEA2145245
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000409304.6/ ENSP00000386412.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-507               AK225823.1         3-509
            508-508             AC073641.3         143309-143309       c
            509-1881            AK225823.1         510-1882
            1882-1882           AC073641.3         120394-120394       c
            1883-1891           AK225823.1         1884-1892
            1892-1892           AC073641.3         120384-120384       c
            1893-2114           AK225823.1         1894-2115
            2115-2143           AI754402.1         18-46               c
            2144-2223           DB325994.1         1-80                c
            2224-2226           AC073641.3         120050-120052       c
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q36.1"
     gene            1..2226
                     /gene="SERPINE2"
                     /gene_synonym="GDN; GDNPF; PI-7; PI7; PN-1; PN1; PNI"
                     /note="serpin family E member 2"
                     /db_xref="GeneID:5270"
                     /db_xref="HGNC:HGNC:8951"
                     /db_xref="MIM:177010"
     exon            1..188
                     /gene="SERPINE2"
                     /gene_synonym="GDN; GDNPF; PI-7; PI7; PN-1; PN1; PNI"
                     /inference="alignment:Splign:2.1.0"
     exon            189..469
                     /gene="SERPINE2"
                     /gene_synonym="GDN; GDNPF; PI-7; PI7; PN-1; PN1; PNI"
                     /inference="alignment:Splign:2.1.0"
     CDS             211..1404
                     /gene="SERPINE2"
                     /gene_synonym="GDN; GDNPF; PI-7; PI7; PN-1; PN1; PNI"
                     /note="isoform b precursor is encoded by transcript
                     variant 2; glial-derived neurite promoting factor;
                     glia-derived nexin; serpin E2; protease nexin I; peptidase
                     inhibitor 7; serpin peptidase inhibitor, clade E (nexin,
                     plasminogen activator inhibitor type 1), member 2"
                     /codon_start=1
                     /product="glia-derived nexin isoform b precursor"
                     /protein_id="NP_001130000.1"
                     /db_xref="CCDS:CCDS46526.1"
                     /db_xref="GeneID:5270"
                     /db_xref="HGNC:HGNC:8951"
                     /db_xref="MIM:177010"
                     /translation="MNWHLPLFLLASVTLPSICSHFNPLSLEELGSNTGIQVFNQIVK
                     SRPHDNIVISPHGIASVLGMLQLGADGRTKKQLAMVMRYGVNGVGKILKKINKAIVSK
                     KNKDIVTVANAVFVKNASEIEVPFVTRNKDVFQCEVRNVNFEDPASACDSINAWVKNE
                     TRDMIDNLLSPDLIDGVLTRLVLVNAVYFKGLWKSRFQPENTKKRTFVAADGKSYQVP
                     MLAQLSVFRCGSTSAPNDLWYNFIELPYHGESISMLIALPTESSTPLSAIIPHISTKT
                     IDSWMSIMVPKRVQVILPKFTAVAQTDLKEPLKVLGITDMFDSSKANFAKITRSENLH
                     VSHILQKAKIEVSEDGTKASAATTAILIARSSPPWFIVDRPFLFFIRHNPTGAVLFMG
                     QINKP"
     exon            470..697
                     /gene="SERPINE2"
                     /gene_synonym="GDN; GDNPF; PI-7; PI7; PN-1; PN1; PNI"
                     /inference="alignment:Splign:2.1.0"
     exon            698..895
                     /gene="SERPINE2"
                     /gene_synonym="GDN; GDNPF; PI-7; PI7; PN-1; PN1; PNI"
                     /inference="alignment:Splign:2.1.0"
     exon            896..1094
                     /gene="SERPINE2"
                     /gene_synonym="GDN; GDNPF; PI-7; PI7; PN-1; PN1; PNI"
                     /inference="alignment:Splign:2.1.0"
     exon            1095..1195
                     /gene="SERPINE2"
                     /gene_synonym="GDN; GDNPF; PI-7; PI7; PN-1; PN1; PNI"
                     /inference="alignment:Splign:2.1.0"
     exon            1196..1282
                     /gene="SERPINE2"
                     /gene_synonym="GDN; GDNPF; PI-7; PI7; PN-1; PN1; PNI"
                     /inference="alignment:Splign:2.1.0"
     exon            1283..1366
                     /gene="SERPINE2"
                     /gene_synonym="GDN; GDNPF; PI-7; PI7; PN-1; PN1; PNI"
                     /inference="alignment:Splign:2.1.0"
     exon            1367..2226
                     /gene="SERPINE2"
                     /gene_synonym="GDN; GDNPF; PI-7; PI7; PN-1; PN1; PNI"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2090..2095
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SERPINE2"
                     /gene_synonym="GDN; GDNPF; PI-7; PI7; PN-1; PN1; PNI"
                     /note="hexamer: AATAAA"
     regulatory      2113..2118
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SERPINE2"
                     /gene_synonym="GDN; GDNPF; PI-7; PI7; PN-1; PN1; PNI"
                     /note="hexamer: AATAAA"
     polyA_site      2118
                     /gene="SERPINE2"
                     /gene_synonym="GDN; GDNPF; PI-7; PI7; PN-1; PN1; PNI"
                     /note="major polyA site"
     regulatory      2140..2145
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SERPINE2"
                     /gene_synonym="GDN; GDNPF; PI-7; PI7; PN-1; PN1; PNI"
                     /note="hexamer: AATACA"
     polyA_site      2146
                     /gene="SERPINE2"
                     /gene_synonym="GDN; GDNPF; PI-7; PI7; PN-1; PN1; PNI"
     polyA_site      2159
                     /gene="SERPINE2"
                     /gene_synonym="GDN; GDNPF; PI-7; PI7; PN-1; PN1; PNI"
     regulatory      2188..2193
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SERPINE2"
                     /gene_synonym="GDN; GDNPF; PI-7; PI7; PN-1; PN1; PNI"
                     /note="hexamer: AATAAA"
     polyA_site      2226
                     /gene="SERPINE2"
                     /gene_synonym="GDN; GDNPF; PI-7; PI7; PN-1; PN1; PNI"
ORIGIN      
        1 gcgctgtggg gctgcccggg ctgcgcggcg tctgcaggcg ccaccgctgc ctctttccgg
       61 ctgtgaccct cctcgccgcc gccgcttggc tgcgtcctcc gactccccgc gccgccgaga
      121 ccaggctccc gctccggttg cggccgcacc gccctccgcg gccgccccct ggggatccag
      181 cgagcgcggt cgtccttggt ggaaggaacc atgaactggc atctccccct cttcctcttg
      241 gcctctgtga cgctgccttc catctgctcc cacttcaatc ctctgtctct cgaggaacta
      301 ggctccaaca cggggatcca ggttttcaat cagattgtga agtcgaggcc tcatgacaac
      361 atcgtgatct ctccccatgg gattgcgtcg gtcctgggga tgcttcagct gggggcggac
      421 ggcaggacca agaagcagct cgccatggtg atgagatacg gcgtaaatgg agttggtaaa
      481 atattaaaga agatcaacaa ggccatcgtc tccaagaaga ataaagacat tgtgacagtg
      541 gctaacgccg tgtttgttaa gaatgcctct gaaattgaag tgccttttgt tacaaggaac
      601 aaagatgtgt tccagtgtga ggtccggaat gtgaactttg aggatccagc ctctgcctgt
      661 gattccatca atgcatgggt taaaaatgaa accagggata tgattgacaa tctgctgtcc
      721 ccagatctta ttgatggtgt gctcaccaga ctggtcctcg tcaacgcagt gtatttcaag
      781 ggtctgtgga aatcacggtt ccaacccgag aacacaaaga aacgcacttt cgtggcagcc
      841 gacgggaaat cctatcaagt gccaatgctg gcccagctct ccgtgttccg gtgtgggtcg
      901 acaagtgccc ccaatgattt atggtacaac ttcattgaac tgccctacca cggggaaagc
      961 atcagcatgc tgattgcact gccgactgag agctccactc cgctgtctgc catcatccca
     1021 cacatcagca ccaagaccat agacagctgg atgagcatca tggtgcccaa gagggtgcag
     1081 gtgatcctgc ccaagttcac agctgtagca caaacagatt tgaaggagcc gctgaaagtt
     1141 cttggcatta ctgacatgtt tgattcatca aaggcaaatt ttgcaaaaat aacaaggtca
     1201 gaaaacctcc atgtttctca tatcttgcaa aaagcaaaaa ttgaagtcag tgaagatgga
     1261 accaaagctt cagcagcaac aactgcaatt ctcattgcaa gatcatcgcc tccctggttt
     1321 atagtagaca gaccttttct gtttttcatc cgacataatc ctacaggtgc tgtgttattc
     1381 atggggcaga taaacaaacc ctgaagagta tacaaaagaa accatgcaaa gcaacgacta
     1441 ctttgctacg aagaaagact cctttcctgc atctttcata gttctgttaa atatttttgt
     1501 acatcgcttc tttttcaaaa ctagttctta ggaacagact cgatgcaagt gtttctgttc
     1561 tgggaggtat tggagggaaa aaacaagcag gatggctgga acactgtact gaggaatgaa
     1621 tagaaaggct tccagatgtc taaaagattc tttaaactac tgaactgtta cctaggttaa
     1681 caaccctgtt gagtatttgc tgtttgtcca gttcaggaat ttttgttttg ttttgtctat
     1741 atgtgcggct tttcagaaga aatttaatca gtgtgacaga aaaaaaaatg ttttatggta
     1801 gcttttactt tttatgaaaa aaaaattatt tgccttttaa attcttttcc cccatccccc
     1861 tccaaagtct tgatagcaag cgttattttg gtggtagaaa cggtgaaatc tctagcctct
     1921 ttgtgttttt gttgttgttg ttgttgttgt tttatataat gcatgtattc actaaaataa
     1981 aatttaaaaa actcctgtct tgctagacaa ggttgctgtt gtgcagtgtg cctgtcacta
     2041 ctggtctgta ctccttggat ttgcattttt gtattttgta caaagtaaaa ataaactgtt
     2101 atgagtagta aaaataaagc tatttctctg ctatttgaaa atacaataga agaaactgag
     2161 ccttttagac attcgtcagc ctcttctaat aaacctttgt actatgtaaa catcaggaaa
     2221 ttcaaa
//
