LOCUS       NM_006215               1665 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens serpin family A member 4 (SERPINA4), transcript
            variant 3, mRNA.
ACCESSION   NM_006215
VERSION     NM_006215.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Kim,H., Suh,G.J., Kwon,W.Y., Kim,K.S., Jung,Y.S., Kim,T. and
            Park,H.
  TITLE     Kallistatin deficiency exacerbates neuronal damage after cardiac
            arrest
  JOURNAL   Sci Rep 14 (1), 4279 (2024)
   PUBMED   38383562
  REMARK    GeneRIF: Kallistatin deficiency exacerbates neuronal damage after
            cardiac arrest.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Wang,Y., Chen,X.Y., Shang,K., Xu,Q., Li,Y.X., DU,Y. and Zhuan,L.
  TITLE     [Relationship of the kallistatin gene expression with male
            spermatogenic dysfunction and its possible mechanism]
  JOURNAL   Zhonghua Nan Ke Xue 28 (11), 976-984 (2022)
   PUBMED   37846113
  REMARK    GeneRIF: [Relationship of the kallistatin gene expression with male
            spermatogenic dysfunction and its possible mechanism].
REFERENCE   3  (bases 1 to 1665)
  AUTHORS   Cho,I., Lee,K.N., Joo,E., Kim,Y.M., Kim,T.E. and Park,K.H.
  TITLE     Plasma E-selectin and kallistatin as predictive markers of
            histologic chorioamnionitis in women with preterm premature rupture
            of membranes
  JOURNAL   Am J Reprod Immunol 88 (3), e13584 (2022)
   PUBMED   35772987
  REMARK    GeneRIF: Plasma E-selectin and kallistatin as predictive markers of
            histologic chorioamnionitis in women with preterm premature rupture
            of membranes.
REFERENCE   4  (bases 1 to 1665)
  AUTHORS   Mao,Y., Zhan,S., Qiao,J., Li,L., Liu,H. and Chen,R.
  TITLE     Kallistatin in follicular fluid of women with endometriosis and its
            correlation with IVF outcome
  JOURNAL   Gynecol Endocrinol 37 (12), 1102-1106 (2021)
   PUBMED   34236276
  REMARK    GeneRIF: Kallistatin in follicular fluid of women with
            endometriosis and its correlation with IVF outcome.
REFERENCE   5  (bases 1 to 1665)
  AUTHORS   Krishna,S.M., Li,J., Wang,Y., Moran,C.S., Trollope,A., Huynh,P.,
            Jose,R., Biros,E., Ma,J. and Golledge,J.
  TITLE     Kallistatin limits abdominal aortic aneurysm by attenuating
            generation of reactive oxygen species and apoptosis
  JOURNAL   Sci Rep 11 (1), 17451 (2021)
   PUBMED   34465809
  REMARK    GeneRIF: Kallistatin limits abdominal aortic aneurysm by
            attenuating generation of reactive oxygen species and apoptosis.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1665)
  AUTHORS   Chen,L.M., Song,Q., Chao,L. and Chao,J.
  TITLE     Cellular localization of tissue kallikrein and kallistatin mRNAs in
            human kidney
  JOURNAL   Kidney Int 48 (3), 690-697 (1995)
   PUBMED   7474653
REFERENCE   7  (bases 1 to 1665)
  AUTHORS   Chai,K.X., Ward,D.C., Chao,J. and Chao,L.
  TITLE     Molecular cloning, sequence analysis, and chromosomal localization
            of the human protease inhibitor 4 (kallistatin) gene (PI4)
  JOURNAL   Genomics 23 (2), 370-378 (1994)
   PUBMED   7835886
REFERENCE   8  (bases 1 to 1665)
  AUTHORS   Chai,K.X., Chen,L.M., Chao,J. and Chao,L.
  TITLE     Kallistatin: a novel human serine proteinase inhibitor. Molecular
            cloning, tissue distribution, and expression in Escherichia coli
  JOURNAL   J Biol Chem 268 (32), 24498-24505 (1993)
   PUBMED   8227002
REFERENCE   9  (bases 1 to 1665)
  AUTHORS   Zhou,G.X., Chao,L. and Chao,J.
  TITLE     Kallistatin: a novel human tissue kallikrein inhibitor.
            Purification, characterization, and reactive center sequence
  JOURNAL   J Biol Chem 267 (36), 25873-25880 (1992)
   PUBMED   1334488
REFERENCE   10 (bases 1 to 1665)
  AUTHORS   Wang,M.Y., Day,J., Chao,L. and Chao,J.
  TITLE     Human kallistatin, a new tissue kallikrein-binding protein:
            purification and characterization
  JOURNAL   Adv Exp Med Biol 247B, 1-8 (1989)
   PUBMED   2558505
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA036536.1, BX248760.1 and BC014992.1.
            
            On Nov 22, 2018 this sequence version replaced NM_006215.3.
            
            Transcript Variant: This variant (3) has an alternate splice site
            in the 5' region, which results in translation initiation at a
            downstream AUG codon, compared to variant 1. The resulting isoform
            (2) has a shorter N-terminus, compared to isoform 1. Both variants
            2 and 3 encode the same isoform 2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189664.66086.1,
                                           SRR5189664.71496.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1970526, SAMEA2142586
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000557004.6/ ENSP00000450838.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-15                DA036536.1         23-37
            16-1616             BX248760.1         1-1601
            1617-1665           BC014992.1         1613-1661
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q32.13"
     gene            1..1665
                     /gene="SERPINA4"
                     /gene_synonym="KAL; kallistatin; KLST; KST; PI-4; PI4"
                     /note="serpin family A member 4"
                     /db_xref="GeneID:5267"
                     /db_xref="HGNC:HGNC:8948"
                     /db_xref="MIM:147935"
     exon            1..53
                     /gene="SERPINA4"
                     /gene_synonym="KAL; kallistatin; KLST; KST; PI-4; PI4"
                     /inference="alignment:Splign:2.1.0"
     exon            54..719
                     /gene="SERPINA4"
                     /gene_synonym="KAL; kallistatin; KLST; KST; PI-4; PI4"
                     /inference="alignment:Splign:2.1.0"
     CDS             71..1354
                     /gene="SERPINA4"
                     /gene_synonym="KAL; kallistatin; KLST; KST; PI-4; PI4"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 3; protease inhibitor 4 (kallistatin); serine (or
                     cysteine) proteinase inhibitor, clade A (alpha-1
                     antiproteinase, antitrypsin), member 4; serpin A4;
                     kallikrein inhibitor; peptidase inhibitor 4; serpin
                     peptidase inhibitor, clade A (alpha-1 antiproteinase,
                     antitrypsin), member 4; testicular secretory protein Li
                     22; sserpin family A member 4"
                     /codon_start=1
                     /product="kallistatin isoform 2 precursor"
                     /protein_id="NP_006206.2"
                     /db_xref="CCDS:CCDS9927.1"
                     /db_xref="GeneID:5267"
                     /db_xref="HGNC:HGNC:8948"
                     /db_xref="MIM:147935"
                     /translation="MHLIDYLLLLLVGLLALSHGQLHVEHDGESCSNSSHQQILETGE
                     GSPSLKIAPANADFAFRFYYLIASETPGKNIFFSPLSISAAYAMLSLGACSHSRSQIL
                     EGLGFNLTELSESDVHRGFQHLLHTLNLPGHGLETRVGSALFLSHNLKFLAKFLNDTM
                     AVYEAKLFHTNFYDTVGTIQLINDHVKKETRGKIVDLVSELKKDVLMVLVNYIYFKAL
                     WEKPFISSRTTPKDFYVDENTTVRVPMMLQDQEHHWYLHDRYLPCSVLRMDYKGDATV
                     FFILPNQGKMREIEEVLTPEMLMRWNNLLRKRNFYKKLELHLPKFSISGSYVLDQILP
                     RLGFTDLFSKWADLSGITKQQKLEASKSFHKATLDVDEAGTEAAAATSFAIKFFSAQT
                     NRHILRFNRPFLVVIFSTSTQSVLFLGKVVDPTKP"
     exon            720..993
                     /gene="SERPINA4"
                     /gene_synonym="KAL; kallistatin; KLST; KST; PI-4; PI4"
                     /inference="alignment:Splign:2.1.0"
     exon            994..1153
                     /gene="SERPINA4"
                     /gene_synonym="KAL; kallistatin; KLST; KST; PI-4; PI4"
                     /inference="alignment:Splign:2.1.0"
     exon            1154..1665
                     /gene="SERPINA4"
                     /gene_synonym="KAL; kallistatin; KLST; KST; PI-4; PI4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1643..1648
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SERPINA4"
                     /gene_synonym="KAL; kallistatin; KLST; KST; PI-4; PI4"
                     /note="hexamer: AGTAAA"
     polyA_site      1665
                     /gene="SERPINA4"
                     /gene_synonym="KAL; kallistatin; KLST; KST; PI-4; PI4"
                     /note="major polyA site"
ORIGIN      
        1 aagacagagc tgagacagcc acccaggggg tccactccag gacagactgt gccgcctgag
       61 agtgcagagg atgcatctta tcgactacct gctcctcctg ctggttggac tactggccct
      121 ttctcatggc cagctgcacg ttgagcatga tggtgagagt tgcagtaaca gctcccacca
      181 gcagattctg gagacaggtg agggctcccc cagcctcaag atagcccctg ccaatgctga
      241 ctttgccttc cgcttctact acctgatcgc ttcggagacc ccggggaaga acatcttttt
      301 ctccccgctg agcatctcgg cggcctacgc catgctttcc ctgggggcct gctcacacag
      361 ccgcagccag atccttgagg gcctgggctt caacctcacc gagctgtctg agtccgatgt
      421 ccataggggc ttccagcacc tcctgcacac tctcaacctc cccggccatg ggctggaaac
      481 acgcgtgggc agtgctctgt tcctgagcca caacctgaag ttccttgcaa aattcctgaa
      541 tgacaccatg gccgtctatg aggctaaact cttccacacc aacttctacg acactgtggg
      601 cacaatccag cttatcaacg accacgtcaa gaaggaaact cgagggaaga ttgtggattt
      661 ggtcagtgag ctcaagaagg acgtcttgat ggtgctggtg aattacattt acttcaaagc
      721 cctgtgggag aaaccattca tttcctcaag gaccactccc aaagacttct atgttgatga
      781 gaacacaaca gtccgggtgc ccatgatgct gcaggaccag gagcatcact ggtatcttca
      841 tgacagatac ttgccctgct cggtgctacg gatggattac aaaggagacg caaccgtgtt
      901 tttcattctc cctaaccaag gcaaaatgag ggagattgaa gaggttctga ctccagagat
      961 gctaatgagg tggaacaact tgttgcggaa gaggaatttt tacaagaagc tagagttgca
     1021 tcttcccaag ttctccattt ctggctccta tgtattagat cagattttgc ccaggctggg
     1081 cttcacggat ctgttctcca agtgggctga cttatccggc atcaccaaac agcaaaaact
     1141 ggaggcatcc aaaagtttcc acaaggccac cttggacgtg gatgaggctg gcaccgaggc
     1201 tgcagcagcc accagcttcg cgatcaaatt cttctctgcc cagaccaatc gccacatcct
     1261 gcgattcaac cggcccttcc ttgtggtgat cttttccacc agcacccaga gtgtcctctt
     1321 tctgggcaag gtcgtcgacc ccacgaaacc atagccctcc cagggctgct catctgttcc
     1381 aagcaggagg atgtggcagg ggagggctgg gagtgagtag ctctgtgttt agagttgggg
     1441 acaaggatga caccgaaggt ccaggagtcc aggacagcag gtgctggccg gtggggagcg
     1501 gggaggggca ctgagatggg cagggcctgg acattccaca ccctggtgct gtgcagcctc
     1561 tggcagagca tccgacctct tggagcaagt ttctgcctct ggaaaggggg cgggcccttt
     1621 tcacaacagg ctggttgtac cgagtaaaca acacgatgcc atgaa
//
