LOCUS       NP_006206                427 aa            linear   PRI 27-APR-2025
DEFINITION  kallistatin isoform 2 precursor [Homo sapiens].
ACCESSION   NP_006206
VERSION     NP_006206.2
DBSOURCE    REFSEQ: accession NM_006215.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 427)
  AUTHORS   Kim,H., Suh,G.J., Kwon,W.Y., Kim,K.S., Jung,Y.S., Kim,T. and
            Park,H.
  TITLE     Kallistatin deficiency exacerbates neuronal damage after cardiac
            arrest
  JOURNAL   Sci Rep 14 (1), 4279 (2024)
   PUBMED   38383562
  REMARK    GeneRIF: Kallistatin deficiency exacerbates neuronal damage after
            cardiac arrest.
            Publication Status: Online-Only
REFERENCE   2  (residues 1 to 427)
  AUTHORS   Wang,Y., Chen,X.Y., Shang,K., Xu,Q., Li,Y.X., DU,Y. and Zhuan,L.
  TITLE     [Relationship of the kallistatin gene expression with male
            spermatogenic dysfunction and its possible mechanism]
  JOURNAL   Zhonghua Nan Ke Xue 28 (11), 976-984 (2022)
   PUBMED   37846113
  REMARK    GeneRIF: [Relationship of the kallistatin gene expression with male
            spermatogenic dysfunction and its possible mechanism].
REFERENCE   3  (residues 1 to 427)
  AUTHORS   Cho,I., Lee,K.N., Joo,E., Kim,Y.M., Kim,T.E. and Park,K.H.
  TITLE     Plasma E-selectin and kallistatin as predictive markers of
            histologic chorioamnionitis in women with preterm premature rupture
            of membranes
  JOURNAL   Am J Reprod Immunol 88 (3), e13584 (2022)
   PUBMED   35772987
  REMARK    GeneRIF: Plasma E-selectin and kallistatin as predictive markers of
            histologic chorioamnionitis in women with preterm premature rupture
            of membranes.
REFERENCE   4  (residues 1 to 427)
  AUTHORS   Mao,Y., Zhan,S., Qiao,J., Li,L., Liu,H. and Chen,R.
  TITLE     Kallistatin in follicular fluid of women with endometriosis and its
            correlation with IVF outcome
  JOURNAL   Gynecol Endocrinol 37 (12), 1102-1106 (2021)
   PUBMED   34236276
  REMARK    GeneRIF: Kallistatin in follicular fluid of women with
            endometriosis and its correlation with IVF outcome.
REFERENCE   5  (residues 1 to 427)
  AUTHORS   Krishna,S.M., Li,J., Wang,Y., Moran,C.S., Trollope,A., Huynh,P.,
            Jose,R., Biros,E., Ma,J. and Golledge,J.
  TITLE     Kallistatin limits abdominal aortic aneurysm by attenuating
            generation of reactive oxygen species and apoptosis
  JOURNAL   Sci Rep 11 (1), 17451 (2021)
   PUBMED   34465809
  REMARK    GeneRIF: Kallistatin limits abdominal aortic aneurysm by
            attenuating generation of reactive oxygen species and apoptosis.
            Publication Status: Online-Only
REFERENCE   6  (residues 1 to 427)
  AUTHORS   Chen,L.M., Song,Q., Chao,L. and Chao,J.
  TITLE     Cellular localization of tissue kallikrein and kallistatin mRNAs in
            human kidney
  JOURNAL   Kidney Int 48 (3), 690-697 (1995)
   PUBMED   7474653
REFERENCE   7  (residues 1 to 427)
  AUTHORS   Chai,K.X., Ward,D.C., Chao,J. and Chao,L.
  TITLE     Molecular cloning, sequence analysis, and chromosomal localization
            of the human protease inhibitor 4 (kallistatin) gene (PI4)
  JOURNAL   Genomics 23 (2), 370-378 (1994)
   PUBMED   7835886
REFERENCE   8  (residues 1 to 427)
  AUTHORS   Chai,K.X., Chen,L.M., Chao,J. and Chao,L.
  TITLE     Kallistatin: a novel human serine proteinase inhibitor. Molecular
            cloning, tissue distribution, and expression in Escherichia coli
  JOURNAL   J Biol Chem 268 (32), 24498-24505 (1993)
   PUBMED   8227002
REFERENCE   9  (residues 1 to 427)
  AUTHORS   Zhou,G.X., Chao,L. and Chao,J.
  TITLE     Kallistatin: a novel human tissue kallikrein inhibitor.
            Purification, characterization, and reactive center sequence
  JOURNAL   J Biol Chem 267 (36), 25873-25880 (1992)
   PUBMED   1334488
REFERENCE   10 (residues 1 to 427)
  AUTHORS   Wang,M.Y., Day,J., Chao,L. and Chao,J.
  TITLE     Human kallistatin, a new tissue kallikrein-binding protein:
            purification and characterization
  JOURNAL   Adv Exp Med Biol 247B, 1-8 (1989)
   PUBMED   2558505
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA036536.1, BX248760.1 and BC014992.1.
            
            On Jun 10, 2002 this sequence version replaced NP_006206.1.
            
            Transcript Variant: This variant (3) has an alternate splice site
            in the 5' region, which results in translation initiation at a
            downstream AUG codon, compared to variant 1. The resulting isoform
            (2) has a shorter N-terminus, compared to isoform 1. Both variants
            2 and 3 encode the same isoform 2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189664.66086.1,
                                           SRR5189664.71496.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1970526, SAMEA2142586
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000557004.6/ ENSP00000450838.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q32.13"
     Protein         1..427
                     /product="kallistatin isoform 2 precursor"
                     /note="protease inhibitor 4 (kallistatin); serine (or
                     cysteine) proteinase inhibitor, clade A (alpha-1
                     antiproteinase, antitrypsin), member 4; serpin A4;
                     kallikrein inhibitor; peptidase inhibitor 4; serpin
                     peptidase inhibitor, clade A (alpha-1 antiproteinase,
                     antitrypsin), member 4; testicular secretory protein Li
                     22; sserpin family A member 4"
                     /calculated_mol_wt=46355
     sig_peptide     1..20
                     /inference="COORDINATES: ab initio prediction:SignalP:6.0"
                     /calculated_mol_wt=2205
     mat_peptide     21..427
                     /product="Kallistatin. /id=PRO_0000032425"
                     /note="propagated from UniProtKB/Swiss-Prot (P29622.3)"
                     /calculated_mol_wt=46355
     Site            33
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (P29622.3)"
     Region          44..426
                     /region_name="serpinA4_KST"
                     /note="serpin family A member 4, kallistatin; cd19552"
                     /db_xref="CDD:381020"
     Site            108
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000269|PubMed:16335952; propagated from
                     UniProtKB/Swiss-Prot (P29622.3)"
     Site            157
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000269|PubMed:12754519,
                     ECO:0000269|PubMed:16335952; propagated from
                     UniProtKB/Swiss-Prot (P29622.3)"
     Site            238
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) (complex) asparagine.
                     /evidence=ECO:0000269|PubMed:16335952,
                     ECO:0000269|PubMed:19139490; propagated from
                     UniProtKB/Swiss-Prot (P29622.3)"
     Site            order(374..385,387..402)
                     /site_type="other"
                     /note="reactive center loop (RCL)"
                     /db_xref="CDD:381020"
     Site            388..389
                     /site_type="other"
                     /note="Reactive bond; propagated from UniProtKB/Swiss-Prot
                     (P29622.3)"
     CDS             1..427
                     /gene="SERPINA4"
                     /gene_synonym="KAL; kallistatin; KLST; KST; PI-4; PI4"
                     /coded_by="NM_006215.4:71..1354"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 3"
                     /db_xref="CCDS:CCDS9927.1"
                     /db_xref="GeneID:5267"
                     /db_xref="HGNC:HGNC:8948"
                     /db_xref="MIM:147935"
ORIGIN      
        1 mhlidyllll lvgllalshg qlhvehdges csnsshqqil etgegspslk iapanadfaf
       61 rfyyliaset pgkniffspl sisaayamls lgacshsrsq ileglgfnlt elsesdvhrg
      121 fqhllhtlnl pghgletrvg salflshnlk flakflndtm avyeaklfht nfydtvgtiq
      181 lindhvkket rgkivdlvse lkkdvlmvlv nyiyfkalwe kpfissrttp kdfyvdentt
      241 vrvpmmlqdq ehhwylhdry lpcsvlrmdy kgdatvffil pnqgkmreie evltpemlmr
      301 wnnllrkrnf ykklelhlpk fsisgsyvld qilprlgftd lfskwadlsg itkqqkleas
      361 ksfhkatldv deagteaaaa tsfaikffsa qtnrhilrfn rpflvvifst stqsvlflgk
      421 vvdptkp
//
