LOCUS       NM_016955               5503 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA
            synthase (SEPSECS), transcript variant 1, mRNA.
ACCESSION   NM_016955 NM_153825
VERSION     NM_016955.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5503)
  AUTHORS   Vishweswaraiah,S., Yilmaz,A., Saiyed,N., Khalid,A., Koladiya,P.R.,
            Pan,X., Macias,S., Robinson,A.C., Mann,D., Green,B.D.,
            Kerseviciute,I., Gordevicius,J., Radhakrishna,U. and Graham,S.F.
  TITLE     Integrative Analysis Unveils the Correlation of Aminoacyl-tRNA
            Biosynthesis Metabolites with the Methylation of the SEPSECS Gene
            in Huntington's Disease Brain Tissue
  JOURNAL   Genes (Basel) 14 (9), 1752 (2023)
   PUBMED   37761892
  REMARK    GeneRIF: Integrative Analysis Unveils the Correlation of
            Aminoacyl-tRNA Biosynthesis Metabolites with the Methylation of the
            SEPSECS Gene in Huntington's Disease Brain Tissue.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5503)
  AUTHORS   Ramadesikan,S., Hickey,S., De Los Reyes,E., Patel,A.D.,
            Franklin,S.J., Brennan,P., Crist,E., Lee,K., White,P.,
            McBride,K.L., Koboldt,D.C. and Wilson,R.K.
  TITLE     Biallelic SEPSECS variants in two siblings with pontocerebellar
            hypoplasia type 2D underscore the relevance of splice-disrupting
            synonymous variants in disease
  JOURNAL   Cold Spring Harb Mol Case Stud 8 (2), a006165 (2022)
   PUBMED   35091508
  REMARK    GeneRIF: Biallelic SEPSECS variants in two siblings with
            pontocerebellar hypoplasia type 2D underscore the relevance of
            splice-disrupting synonymous variants in disease.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5503)
  AUTHORS   Schoenmakers,E. and Chatterjee,K.
  TITLE     Human Genetic Disorders Resulting in Systemic Selenoprotein
            Deficiency
  JOURNAL   Int J Mol Sci 22 (23), 12927 (2021)
   PUBMED   34884733
  REMARK    GeneRIF: Human Genetic Disorders Resulting in Systemic
            Selenoprotein Deficiency.
            Review article
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5503)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 5503)
  AUTHORS   Olson,H.E., Kelly,M., LaCoursiere,C.M., Pinsky,R., Tambunan,D.,
            Shain,C., Ramgopal,S., Takeoka,M., Libenson,M.H., Julich,K.,
            Loddenkemper,T., Marsh,E.D., Segal,D., Koh,S., Salman,M.S.,
            Paciorkowski,A.R., Yang,E., Bergin,A.M., Sheidley,B.R. and
            Poduri,A.
  TITLE     Genetics and genotype-phenotype correlations in early onset
            epileptic encephalopathy with burst suppression
  JOURNAL   Ann Neurol 81 (3), 419-429 (2017)
   PUBMED   28133863
  REMARK    GeneRIF: Epileptic encephalopathy with burst suppression without
            brain malformations is associated with pathogenic variation in
            SEPSECS.
REFERENCE   6  (bases 1 to 5503)
  AUTHORS   Xu,X.M., Mix,H., Carlson,B.A., Grabowski,P.J., Gladyshev,V.N.,
            Berry,M.J. and Hatfield,D.L.
  TITLE     Evidence for direct roles of two additional factors, SECp43 and
            soluble liver antigen, in the selenoprotein synthesis machinery
  JOURNAL   J Biol Chem 280 (50), 41568-41575 (2005)
   PUBMED   16230358
REFERENCE   7  (bases 1 to 5503)
  AUTHORS   Herkel,J., Heidrich,B., Nieraad,N., Wies,I., Rother,M. and
            Lohse,A.W.
  TITLE     Fine specificity of autoantibodies to soluble liver antigen and
            liver/pancreas
  JOURNAL   Hepatology 35 (2), 403-408 (2002)
   PUBMED   11826415
REFERENCE   8  (bases 1 to 5503)
  AUTHORS   Volkmann,M., Martin,L., Baurle,A., Heid,H., Strassburg,C.P.,
            Trautwein,C., Fiehn,W. and Manns,M.P.
  TITLE     Soluble liver antigen: isolation of a 35-kd recombinant protein
            (SLA-p35) specifically recognizing sera from patients with
            autoimmune hepatitis
  JOURNAL   Hepatology 33 (3), 591-596 (2001)
   PUBMED   11230739
REFERENCE   9  (bases 1 to 5503)
  AUTHORS   Costa,M., Rodriguez-Sanchez,J.L., Czaja,A.J. and Gelpi,C.
  TITLE     Isolation and characterization of cDNA encoding the antigenic
            protein of the human tRNP(Ser)Sec complex recognized by
            autoantibodies from patients withtype-1 autoimmune hepatitis
  JOURNAL   Clin Exp Immunol 121 (2), 364-374 (2000)
   PUBMED   10931155
REFERENCE   10 (bases 1 to 5503)
  AUTHORS   Wies,I., Brunner,S., Henninger,J., Herkel,J., Kanzler,S., Meyer zum
            Buschenfelde,K.H. and Lohse,A.W.
  TITLE     Identification of target antigen for SLA/LP autoantibodies in
            autoimmune hepatitis
  JOURNAL   Lancet 355 (9214), 1510-1515 (2000)
   PUBMED   10801173
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK292476.1, BX648976.1,
            DA155173.1, CN429794.1, AC007073.2 and AW515989.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_016955.3.
            
            Summary: The amino acid selenocysteine is the only amino acid that
            does not have its own tRNA synthetase. Instead, this amino acid is
            synthesized on its cognate tRNA in a three step process. The
            protein encoded by this gene catalyzes the third step in the
            process, the conversion of O-phosphoseryl-tRNA(Sec) to
            selenocysteinyl-tRNA(Sec).[provided by RefSeq, Mar 2011].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803617.158496.1,
                                           SRR1660807.126448.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2467146 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000382103.7/ ENSP00000371535.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-870               AK292476.1         134-1003
            871-1635            BX648976.1         865-1629
            1636-1828           AK292476.1         1769-1961
            1829-2392           BX648976.1         1820-2383
            2393-2802           DA155173.1         149-558
            2803-3313           CN429794.1         114-624
            3314-4516           BX648976.1         3305-4507
            4517-5452           AC007073.2         33240-34175
            5453-5503           AW515989.1         13-63               c
FEATURES             Location/Qualifiers
     source          1..5503
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4p15.2"
     gene            1..5503
                     /gene="SEPSECS"
                     /gene_synonym="LP; PCH2D; SecS; SLA; SLA-p35; SLA/LP"
                     /note="Sep (O-phosphoserine) tRNA:Sec (selenocysteine)
                     tRNA synthase"
                     /db_xref="GeneID:51091"
                     /db_xref="HGNC:HGNC:30605"
                     /db_xref="MIM:613009"
     exon            1..194
                     /gene="SEPSECS"
                     /gene_synonym="LP; PCH2D; SecS; SLA; SLA-p35; SLA/LP"
                     /inference="alignment:Splign:2.1.0"
     CDS             81..1586
                     /gene="SEPSECS"
                     /gene_synonym="LP; PCH2D; SecS; SLA; SLA-p35; SLA/LP"
                     /EC_number="2.9.1.2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     soluble liver antigen/liver pancreas antigen;
                     O-phosphoseryl-tRNA(Sec) selenium transferase; SLA/LP
                     autoantigen; liver-pancreas antigen; UGA suppressor
                     tRNA-associated protein; tRNA(Ser/Sec)-associated
                     antigenic protein"
                     /codon_start=1
                     /product="O-phosphoseryl-tRNA(Sec) selenium transferase
                     isoform 1"
                     /protein_id="NP_058651.3"
                     /db_xref="CCDS:CCDS3432.2"
                     /db_xref="GeneID:51091"
                     /db_xref="HGNC:HGNC:30605"
                     /db_xref="MIM:613009"
                     /translation="MNRESFAAGERLVSPAYVRQGCEARRSHEHLIRLLLEKGKCPEN
                     GWDESTLELFLHELAIMDSNNFLGNCGVGEREGRVASALVARRHYRFIHGIGRSGDIS
                     AVQPKAAGSSLLNKITNSLVLDIIKLAGVHTVANCFVVPMATGMSLTLCFLTLRHKRP
                     KAKYIIWPRIDQKSCFKSMITAGFEPVVIENVLEGDELRTDLKAVEAKVQELGPDCIL
                     CIHSTTSCFAPRVPDRLEELAVICANYDIPHIVNNAYGVQSSKCMHLIQQGARVGRID
                     AFVQSLDKNFMVPVGGAIIAGFNDSFIQEISKMYPGRASASPSLDVLITLLSLGSNGY
                     KKLLKERKEMFSYLSNQIKKLSEAYNERLLHTPHNPISLAMTLKTLDEHRDKAVTQLG
                     SMLFTRQVSGARVVPLGSMQTVSGYTFRGFMSHTNNYPCAYLNAASAIGMKMQDVDLF
                     IKRLDRCLKAVRKERSKESDDNYDKTEDVDIEEMALKLDNVLLDTYQDASS"
     exon            195..349
                     /gene="SEPSECS"
                     /gene_synonym="LP; PCH2D; SecS; SLA; SLA-p35; SLA/LP"
                     /inference="alignment:Splign:2.1.0"
     exon            350..468
                     /gene="SEPSECS"
                     /gene_synonym="LP; PCH2D; SecS; SLA; SLA-p35; SLA/LP"
                     /inference="alignment:Splign:2.1.0"
     exon            469..627
                     /gene="SEPSECS"
                     /gene_synonym="LP; PCH2D; SecS; SLA; SLA-p35; SLA/LP"
                     /inference="alignment:Splign:2.1.0"
     exon            628..781
                     /gene="SEPSECS"
                     /gene_synonym="LP; PCH2D; SecS; SLA; SLA-p35; SLA/LP"
                     /inference="alignment:Splign:2.1.0"
     exon            782..884
                     /gene="SEPSECS"
                     /gene_synonym="LP; PCH2D; SecS; SLA; SLA-p35; SLA/LP"
                     /inference="alignment:Splign:2.1.0"
     exon            885..1014
                     /gene="SEPSECS"
                     /gene_synonym="LP; PCH2D; SecS; SLA; SLA-p35; SLA/LP"
                     /inference="alignment:Splign:2.1.0"
     exon            1015..1106
                     /gene="SEPSECS"
                     /gene_synonym="LP; PCH2D; SecS; SLA; SLA-p35; SLA/LP"
                     /inference="alignment:Splign:2.1.0"
     exon            1107..1200
                     /gene="SEPSECS"
                     /gene_synonym="LP; PCH2D; SecS; SLA; SLA-p35; SLA/LP"
                     /inference="alignment:Splign:2.1.0"
     exon            1201..1291
                     /gene="SEPSECS"
                     /gene_synonym="LP; PCH2D; SecS; SLA; SLA-p35; SLA/LP"
                     /inference="alignment:Splign:2.1.0"
     exon            1292..5503
                     /gene="SEPSECS"
                     /gene_synonym="LP; PCH2D; SecS; SLA; SLA-p35; SLA/LP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1881..1886
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SEPSECS"
                     /gene_synonym="LP; PCH2D; SecS; SLA; SLA-p35; SLA/LP"
                     /note="hexamer: AGTAAA"
     polyA_site      1908
                     /gene="SEPSECS"
                     /gene_synonym="LP; PCH2D; SecS; SLA; SLA-p35; SLA/LP"
                     /note="major polyA site"
     regulatory      3457..3462
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SEPSECS"
                     /gene_synonym="LP; PCH2D; SecS; SLA; SLA-p35; SLA/LP"
                     /note="hexamer: AATAAA"
     polyA_site      3494
                     /gene="SEPSECS"
                     /gene_synonym="LP; PCH2D; SecS; SLA; SLA-p35; SLA/LP"
     regulatory      5463..5468
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SEPSECS"
                     /gene_synonym="LP; PCH2D; SecS; SLA; SLA-p35; SLA/LP"
                     /note="hexamer: ATTATA"
     regulatory      5484..5489
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SEPSECS"
                     /gene_synonym="LP; PCH2D; SecS; SLA; SLA-p35; SLA/LP"
                     /note="hexamer: AATAAA"
     polyA_site      5485
                     /gene="SEPSECS"
                     /gene_synonym="LP; PCH2D; SecS; SLA; SLA-p35; SLA/LP"
     polyA_site      5503
                     /gene="SEPSECS"
                     /gene_synonym="LP; PCH2D; SecS; SLA; SLA-p35; SLA/LP"
                     /note="major polyA site"
ORIGIN      
        1 gagttgcatt ctggttccgt cgtgtgtctg gcgtgtggag ctagtgctcc cgcttattcg
       61 ccactgtcgc caccgctgtc atgaaccgcg agagcttcgc ggcgggagag cggctggtgt
      121 cgccggctta cgtgcggcag ggctgtgagg cccgccgctc gcatgagcac ctcatacggc
      181 tgcttctgga gaagggcaag tgtccagaga atggctggga tgaaagtaca cttgaactct
      241 ttttacatga acttgcaatc atggacagca acaatttctt aggcaattgt ggtgtgggag
      301 aaagggaagg gagagtggca tccgcactgg ttgctcgtcg tcattacagg ttcattcatg
      361 gcattggacg atccggtgat atttctgctg tgcaaccaaa agctgcaggc tctagccttt
      421 tgaacaaaat taccaattct ttggtcctgg acattataaa gctggctggt gtccatacag
      481 tagccaactg ctttgtagtt cctatggcaa ctggtatgag tctaactctg tgtttcttaa
      541 cattacgaca caaaagacca aaggcaaagt atattatatg gccacgaata gaccagaagt
      601 cctgctttaa atccatgatc actgcaggtt ttgagcctgt ggtgatagaa aatgttttgg
      661 aaggtgacga gctgcgtaca gacctgaaag cagtggaggc taaagtccag gaacttgggc
      721 ctgattgcat tctgtgtatt cattctacta catcctgttt tgctccaagg gtgcctgata
      781 gattagaaga actggctgtg atttgtgcta attatgacat tccacatata gttaataatg
      841 cttatggagt gcagtcttca aagtgtatgc atctcattca gcagggggct cgagttggta
      901 gaatagatgc ttttgttcag agcttggaca aaaattttat ggttccagta ggtggtgcta
      961 taattgctgg ctttaatgat tcattcattc aggaaatcag caagatgtat ccaggaagag
     1021 cttcagcttc accttcttta gatgtcctta ttactttatt gtcacttgga tcaaatggct
     1081 ataagaagct actaaaagaa agaaaggaaa tgttttcata tttgtccaac caaataaaga
     1141 agttgtcaga agcctacaat gaaagactgt tgcatacacc tcacaatccc atatctttag
     1201 ctatgacact taaaacacta gatgaacacc gtgacaaagc tgtcactcag cttggctcga
     1261 tgctttttac cagacaggtt tctggagcca gggttgtgcc tcttgggtcc atgcaaactg
     1321 tgagtggcta tactttcaga ggctttatgt cacatacaaa taattaccct tgtgcttacc
     1381 tcaatgctgc atcagccatc ggaatgaaga tgcaggatgt ggacctgttc ataaagagac
     1441 ttgacaggtg tttaaaggca gtaagaaaag aacgaagtaa agagagtgat gacaattatg
     1501 acaaaactga agatgtggat attgaagaaa tggctttaaa actagataat gtacttcttg
     1561 acacatacca ggatgcttct tcatgacatg cgaagggttt cttcttgatc atttgaaaga
     1621 aatgataagg ctgtagtaca agcaagcagt ttaaagataa gacttgagat ttttgaattg
     1681 agaatttcat gggaatattc agtcaaggag cagactttgg tctgaactag ccattgatga
     1741 ttgttaagtt tcccattctt agactgcatc aatccttgtg atccttaaca ggttataata
     1801 tacaattagc atttttctaa ttgttagaat atctgtcaat taagcatcat gactcagata
     1861 atttttacta gtgtaatgac agtaaaatta cttcaattct ctttgaaatg tagagtgctt
     1921 cctcctaacc attctttacc gtttctgatc acacagaatt gagaaagatc tcttaggcca
     1981 ctagttacta gttgtttgtt gagatgtgta agtcacagtg tgcctgtaaa atgggcattc
     2041 cccaaggaag cagagtcaag gtataaactt tcgttttgac tcaaaagcag tgggtctcaa
     2101 ctggggatga ttcgcttatc tgaacagcat ctgcagacat ttttggttgt cacaactggt
     2161 gggatgctgt taacatctag aggatagagg ccagggtgct actcaacatc ctacaatgca
     2221 tgggacaact cccacaacaa agaattatcc agcccaaaat gtcattagtg ctgaggttga
     2281 gaaatactcc tctaaagtag ataaactcct tgagtaaaga gaagtttacc atagcaactt
     2341 tcagtagtac ttcaaagaag atagctgtat aaatgtcatc aaactatact atatagagaa
     2401 tcttaagtga taaccagggt cacggattcc aaacatgtca ttataaattg ttttatatgg
     2461 tgctcactgg tgcatttttc cttttggata agggaaaaca ttattccact tactgttttt
     2521 gctttaagca gcctgcatat attggttagt ttgttcagat gttgtgcaca gacatcatca
     2581 aaattagcta acatgggtat ttttgaacat atttttgcca ggaagatgaa aatcacatag
     2641 tatgataaac aggtgtattc ccctctactt gcccatgtgc tcatttttct gagtctgaaa
     2701 ttccagactt actggcttta acaataattt ttgttgttaa tttaagaaat agacttactt
     2761 aagactattt taagaaatag agatgtcata gcctgaagat ttactgaagc ttctgaacat
     2821 gccaccatgc caaagctatg tttctcaaac ccagctctgt ggatcatgtt cctaaaagat
     2881 gctcatgggg agaaaatgtt tacagacaaa taattttggg agctgccttt gcaaacctgc
     2941 ctgttggcat attgaaggct ctaagaattc cttcactaaa taacccagtt ttaatatatc
     3001 cttatccaaa gagaaactca tttttttaaa gtgaaatatt tgttaacagc ccattgaaca
     3061 tacttttaaa aacactatgg tagagaatgc tgtgttgcct tctgggctgc atttttctta
     3121 ttgatttgga attgtaacct atgtatgaga aagggatttt ctggtatttt ggtgacgttg
     3181 tacattgtcc cagactgttt gtaaacatga ggtcagacat gtgtaaggta gacgcctatg
     3241 gccactggct tgaccacctt ggaagtcaat gactattttg agatgaagta tttaactatg
     3301 aatatcatga aggttattta aaaatgtatt tattttatct ttggggttca aacattaaaa
     3361 aaatttacat aggtgtagta actcatttca agggaataaa tgctggatta aaaagtgtaa
     3421 atttttcatg agaatttggg actaaattgc tgaaccaata aagctgaaga gttgcaacct
     3481 ctagagtcaa aaaagggatt gaaatgttgt tgcaaatgta aatgctggca gtgcagcttg
     3541 ctatacatgt aagccaaaat tgttttatag aacaagtgat ctatattgtt gtgatcatca
     3601 tttagcttaa gaaaggccag agctgggaga gtagcaaatg ttttctcatc tgcagtgtat
     3661 cagcagttct tgggcttcag ctgctgaata gataataatt ctaattgaac ataaattaca
     3721 tgaactcaca tgtgaatgat agatatgtta caatatttca ttcctgatgc agaaaatcag
     3781 ctgtggtttt tattattaac cctttctttt tcagtggttt tggaattcta gctctattcc
     3841 tcctggctta aaatttgagg gcatagaatt cacaagagtt tttgaagagt tctgtctatt
     3901 caggctttcc tttgtcccca ccctagtgtc attttcactc actcccattt ctacacataa
     3961 gtattaaaaa ggatgtattt attataattc gctagtactg attggtaagc ctcataattg
     4021 gcctttgtta tctttgtact gtgacgaagg cagtggatga tacattacca atcattgccg
     4081 aagataacct cttctatctt tttgacccaa ctacccttat taaaactatc aggtaaaata
     4141 ttggagttag gtttttaaag tcattgggct gtggcagtgt gcttagaaaa gaaaatggtg
     4201 gaacatagaa tattcttttg gtcactacac aatgtgttac ttatacatgg cagggaaggg
     4261 aattttaaat caaactcgca gtttggttct taggtcctct gctgatgaat tctcgctcta
     4321 tcagggaatg atatgtgacg ccagccttag gaacctggtg ttcaactgta aatctttcta
     4381 tttagctttt tgtattagtt gaatgaagtt atagaatatg aattattgaa tacagttctg
     4441 ttcagccaat aggcacttga tatacagaat ggtaaattta aatataaatt aaatttttct
     4501 aatattatga tcagcacttc actgtggttc tgtgtcttta accacctcat aataagaact
     4561 gttattgagg actgggctac agaatgtgct tagactacat ctatggcttc catagatttt
     4621 agtaaaattt aaacagataa tttgtgttta ctgctgatgt tactttaata ttgaattagt
     4681 agttcatcct gagagttata tttcacagag gaaaataacc aacagctgaa aacaacaata
     4741 ataaagctct ggtgagtctg actcatactc ggagaaagtt tttggaatac atacacattt
     4801 ggtgcatata agagttgggt ttccttttcc cactttctga gatgtactaa cctctgccag
     4861 acccttaaat gattttgtta tgttttgcat ttttgaggcc aaccttattt tgtaactgaa
     4921 aaaagtccag ttttgcatac acagaaattt ggtcatttta gaagtctcac aacctgaata
     4981 atttgataat aattgaatat gctattaaca gctttttaag aaaaggttct attagtcagt
     5041 tttttccttg tctgtcatat tatgttggtt aatgtctctt atggccaatc ttagtaaaca
     5101 gacctaacac tcactgcatc cttttgtgcc ttacttttta ctttcctttt gcccttttgg
     5161 tatgtgcgca tgtaagatgg taagtttaag gctaatgaat atatgtttgt aatttcctac
     5221 tcactactct gcagttaaag ctaaggcctt aggcaaaatc tagaaatgtt tatattgttt
     5281 tgttttttat caagtggtgt caacttgaga aatacaaaga aaggctatgc ttaactgtat
     5341 aaccgtgggg gattttagcc atgagaatat gaagctgatg tcagcctgct aacctgtttt
     5401 accaaaagga aaagatgttt ccaaaaatgt tttcggtgta gactgtttaa agatgaaaat
     5461 aaattatata atttattata tgaaataaat ttttgataag tca
//
