LOCUS       NM_145204               4178 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens SUMO peptidase family member, NEDD8 specific (SENP8),
            transcript variant 2, mRNA.
ACCESSION   NM_145204
VERSION     NM_145204.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 4178)
  AUTHORS   Bailly,A.P., Perrin,A., Serrano-Macia,M., Maghames,C.,
            Leidecker,O., Trauchessec,H., Martinez-Chantar,M.L., Gartner,A. and
            Xirodimas,D.P.
  TITLE     The Balance between Mono- and NEDD8-Chains Controlled by NEDP1 upon
            DNA Damage Is a Regulatory Module of the HSP70 ATPase Activity
  JOURNAL   Cell Rep 29 (1), 212-224 (2019)
   PUBMED   31577950
  REMARK    GeneRIF: The Balance between Mono- and NEDD8-Chains Controlled by
            NEDP1 upon DNA Damage Is a Regulatory Module of the HSP70 ATPase
            Activity.
REFERENCE   3  (bases 1 to 4178)
  AUTHORS   Guan,J., Yu,S. and Zheng,X.
  TITLE     NEDDylation antagonizes ubiquitination of proliferating cell
            nuclear antigen and regulates the recruitment of polymerase eta in
            response to oxidative DNA damage
  JOURNAL   Protein Cell 9 (4), 365-379 (2018)
   PUBMED   28831681
  REMARK    GeneRIF: Impairment of NEDDylation by Ubc12 knockout enhances PCNA
            ubiquitination and promotes PCNA-poleta interaction, while
            up-regulation of NEDDylation by NEDD8 overexpression or NEDP1
            deletion reduces the excessive accumulation of ubiquitinated PCNA.
            Moreover, Ubc12 knockout decreases cell sensitivity to H2O2-induced
            oxidative stress, but NEDP1 deletion aggravates this sensitivity
REFERENCE   4  (bases 1 to 4178)
  AUTHORS   Coleman,K.E., Bekes,M., Chapman,J.R., Crist,S.B., Jones,M.J.,
            Ueberheide,B.M. and Huang,T.T.
  TITLE     SENP8 limits aberrant neddylation of NEDD8 pathway components to
            promote cullin-RING ubiquitin ligase function
  JOURNAL   Elife 6, e24325 (2017)
   PUBMED   28475037
  REMARK    GeneRIF: Furthermore, the authors characterized SENP8/DEN1 as the
            protease that counteracts Ubc12 auto-neddylation, and observed
            aberrant neddylation of Ubc12 and other NEDD8 conjugation pathway
            components in SENP8-deficient cells.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4178)
  AUTHORS   Tuccilli,C., Baldini,E., Sorrenti,S., Di Gioia,C., Bosco,D.,
            Ascoli,V., Mian,C., Barollo,S., Rendina,R., Coccaro,C., Pepe,M.,
            Catania,A., Bononi,M., Tartaglia,F., De Antoni,E., D'Armiento,M.
            and Ulisse,S.
  TITLE     PAPILLARY THYROID CANCER IS CHARACTERIZED BY ALTERED EXPRESSION OF
            GENES INVOLVED IN THE SUMOYLATION PROCESS
  JOURNAL   J Biol Regul Homeost Agents 29 (3), 655-662 (2015)
   PUBMED   26403403
  REMARK    GeneRIF: the expression of SENP8, SAE1, PIAS1, PIAS2 and ZMIZ1 is
            deregulated in the majority of PTC tissues, likely contributing to
            the PTC phenotype.
REFERENCE   6  (bases 1 to 4178)
  AUTHORS   Reverter,D., Wu,K., Erdene,T.G., Pan,Z.Q., Wilkinson,K.D. and
            Lima,C.D.
  TITLE     Structure of a complex between Nedd8 and the Ulp/Senp protease
            family member Den1
  JOURNAL   J Mol Biol 345 (1), 141-151 (2005)
   PUBMED   15567417
  REMARK    GeneRIF: X-ray structures of the human Nedd8-specific protease,
            Den1, in a complex with the inhibitor Nedd8 aldehyde, thus
            revealing a model for the tetrahedral transition state intermediate
            generated during proteolysis
REFERENCE   7  (bases 1 to 4178)
  AUTHORS   Xirodimas,D.P., Saville,M.K., Bourdon,J.C., Hay,R.T. and Lane,D.P.
  TITLE     Mdm2-mediated NEDD8 conjugation of p53 inhibits its transcriptional
            activity
  JOURNAL   Cell 118 (1), 83-97 (2004)
   PUBMED   15242646
REFERENCE   8  (bases 1 to 4178)
  AUTHORS   Wu,K., Yamoah,K., Dolios,G., Gan-Erdene,T., Tan,P., Chen,A.,
            Lee,C.G., Wei,N., Wilkinson,K.D., Wang,R. and Pan,Z.Q.
  TITLE     DEN1 is a dual function protease capable of processing the C
            terminus of Nedd8 and deconjugating hyper-neddylated CUL1
  JOURNAL   J Biol Chem 278 (31), 28882-28891 (2003)
   PUBMED   12759363
  REMARK    GeneRIF: results suggest a unique role for NEDD8-specific protease
            1(DEN1) in regulating the modification of cullin 1 by Nedd8 protein
REFERENCE   9  (bases 1 to 4178)
  AUTHORS   Gan-Erdene,T., Nagamalleswari,K., Yin,L., Wu,K., Pan,Z.Q. and
            Wilkinson,K.D.
  TITLE     Identification and characterization of DEN1, a deneddylase of the
            ULP family
  JOURNAL   J Biol Chem 278 (31), 28892-28900 (2003)
   PUBMED   12759362
REFERENCE   10 (bases 1 to 4178)
  AUTHORS   Mendoza,H.M., Shen,L.N., Botting,C., Lewis,A., Chen,J., Ink,B. and
            Hay,R.T.
  TITLE     NEDP1, a highly conserved cysteine protease that deNEDDylates
            Cullins
  JOURNAL   J Biol Chem 278 (28), 25637-25643 (2003)
   PUBMED   12730221
  REMARK    GeneRIF: NEDP1 is likely to play an important role in
            ubiquitin-mediated proteolysis by controlling the activity of SCF
            complexes
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK292298.1, AY008293.1 and
            AC020779.10.
            
            On Nov 23, 2018 this sequence version replaced NM_145204.3.
            
            Summary: This gene encodes a cysteine protease that is a member of
            the sentrin-specific protease family. The encoded protein is
            involved in processing and deconjugation of the ubiquitin-like
            protein termed, neural precursor cell expressed developmentally
            downregulated 8. Alternate splicing results in multiple transcript
            variants.[provided by RefSeq, Oct 2009].
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1, 2, 3, 4 and 5 encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.183132.1,
                                           SRR1660807.76168.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000340912.6/ ENSP00000340505.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-287               AK292298.1         116-402
            288-1111            AY008293.1         365-1188
            1112-4178           AC020779.10        77517-80583
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q23"
     gene            1..4178
                     /gene="SENP8"
                     /gene_synonym="DEN1; NEDP1; PRSC2"
                     /note="SUMO peptidase family member, NEDD8 specific"
                     /db_xref="GeneID:123228"
                     /db_xref="HGNC:HGNC:22992"
                     /db_xref="MIM:608659"
     exon            1..62
                     /gene="SENP8"
                     /gene_synonym="DEN1; NEDP1; PRSC2"
                     /inference="alignment:Splign:2.1.0"
     exon            63..4178
                     /gene="SENP8"
                     /gene_synonym="DEN1; NEDP1; PRSC2"
                     /inference="alignment:Splign:2.1.0"
     CDS             110..748
                     /gene="SENP8"
                     /gene_synonym="DEN1; NEDP1; PRSC2"
                     /EC_number="3.4.22.68"
                     /note="NEDD8-specific protease 1; deneddylase 1; SUMO
                     sentrin specific protease family member 8; NEDD8
                     specific-protease cysteine 2; SUMO/sentrin peptidase
                     family member, NEDD8 specific; protease, cysteine 2;
                     SUMO/sentrin specific peptidase family member 8"
                     /codon_start=1
                     /product="sentrin-specific protease 8"
                     /protein_id="NP_660205.3"
                     /db_xref="CCDS:CCDS10240.1"
                     /db_xref="GeneID:123228"
                     /db_xref="HGNC:HGNC:22992"
                     /db_xref="MIM:608659"
                     /translation="MDPVVLSYMDSLLRQSDVSLLDPPSWLNDHIIGFAFEYFANSQF
                     HDCSDHVSFISPEVTQFIKCTSNPAEIAMFLEPLDLPNKRVVFLAINDNSNQAAGGTH
                     WSLLVYLQDKNSFFHYDSHSRSNSVHAKQVAEKLEAFLGRKGDKLAFVEEKAPAQQNS
                     YDCGMYVICNTEALCQNFFRQQTESLLQLLTPAYITKKRGEWKDLITTLAKK"
     regulatory      1428..1433
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SENP8"
                     /gene_synonym="DEN1; NEDP1; PRSC2"
                     /note="hexamer: ATTAAA"
     polyA_site      1456
                     /gene="SENP8"
                     /gene_synonym="DEN1; NEDP1; PRSC2"
                     /note="major polyA site"
     regulatory      4157..4162
                     /regulatory_class="polyA_signal_sequence"
                     /gene="SENP8"
                     /gene_synonym="DEN1; NEDP1; PRSC2"
                     /note="hexamer: AATAAA"
     polyA_site      4178
                     /gene="SENP8"
                     /gene_synonym="DEN1; NEDP1; PRSC2"
                     /note="major polyA site"
ORIGIN      
        1 gtcgctttcc ggccaacaca gaggtgcctg aaggctggtt ggggtggtga ggcccgaggc
       61 agctcttgtt cagcttctgg aatttctgag cagccctcgt cagtacaaga tggaccccgt
      121 agtcttgagt tacatggaca gtctactgcg gcaatcagat gtctcactat tggatccgcc
      181 aagctggctc aatgaccata ttattgggtt tgcgtttgag tactttgcca acagtcagtt
      241 tcatgactgc tctgatcacg tcagtttcat cagccctgaa gtcacccagt tcatcaagtg
      301 cactagcaac ccagcagaga ttgccatgtt ccttgaacca ctggacctcc ccaacaagag
      361 agttgtattt ttagccatca atgataactc caaccaggca gctggaggaa cccactggag
      421 tttattggtc tacctccaag ataaaaatag cttttttcat tatgattccc atagcaggag
      481 caactcagtt cacgcaaagc aggtagcaga gaaactggag gctttcttag gcagaaaagg
      541 agacaaactg gcctttgtgg aagagaaagc ccctgcccaa caaaacagct atgactgtgg
      601 gatgtacgtg atatgtaaca ctgaggcctt gtgtcagaac ttctttaggc aacagacaga
      661 atcactgctg cagctactca cccctgcata catcacaaag aagaggggag aatggaaaga
      721 tctcattacc acacttgcta aaaagtagct attgaagtat atttgcgact tttgaaggct
      781 cctctttctg cccttcccca tttgttggat ggctgcaatc tcagtgcctg agggaagatg
      841 cctagtagag gaaagcttaa tactcttttt cctgaaagaa tatcatcctc tgcattatcc
      901 ccatggaacg tttcacttta accctgactg gggagcaata tgttctgtga aaatatcttg
      961 aaattgtaca ccaaaacctt acaaccaact tatttgaaca tttattacac acagggttta
     1021 cgtaagactt ttcttattgg tatataatta atttcctttg gtctccctta tccacattgg
     1081 cttattctgg aggaaaagca gtgatctgta aaacaaatca agaatatatt aaatctagag
     1141 gaatgcagag aagaaaacta taaaacagaa ccaaaaactt gttgcacagc ctacataatt
     1201 aagagatcaa ctggctggaa gcagatcaag gcctaacttc attcaagacc taaatattat
     1261 gagactcagt tattcggttt tatgtgacat ctcttccatt caccatgcac aggcttttcc
     1321 agctatctat ataatgtttg caaatatttg ataaagatga tgttacccta tcttcctcca
     1381 tctgattcct ggaatgcttg aagaaagggg aaatcttgag taacctcatt aaaattaatg
     1441 tctggtggac ctctcagttt gctcactgat tgggtagact tgctactctg gattaataac
     1501 tgcttttctc tgccttgtct attagccatg cacacacttc ctccttatat ccaaagttct
     1561 ttggttctaa aattcatagt tgataccttc ccaaggttac ctgttgtttc agataaacat
     1621 ttgttcttcc tgatagttat aggttttcct ctctgtcctt aattctggca gaattgtttt
     1681 tagttatgtc atggtaattt caggatgatt tttgtatctg aaacttaaca gacttagagt
     1741 tggtttgttt tttaaatata aaggaagaaa agtgaccttt ctcagccctt ttacttaatt
     1801 aaaaatgcag ggaatgtaat ttgtaaaatg tggaaccaag agtagaacga aatactatcc
     1861 ctccagaagc actgatgtgt ttcagttgtt tcaaatagta aacaaccaga tgacatgtta
     1921 ggtacacaaa ttaattctat tcctaatgag aattagattg caaggcctat gagacttgaa
     1981 aaccaaagat gagcttcagc catgaaactt atttcagcta cctccccagc ctaaccctgc
     2041 ccccagccct catggcccaa aataacctca ccactcagtg accacagaga aagtaacttg
     2101 gttcctttta gccgagaagg gaaaacacag taagggtaaa taagtacagg ttacatagca
     2161 ggcctgagtg agcaggagct gaaactaaaa gtcaaagaga aagctatcaa aaaaagtttc
     2221 catttcaagt cggtacattt tggataaaaa ccagtcctaa tggaagatgg ggctaggaaa
     2281 aagattagga actggcagtc atccatagag gtgaaataca gagggaggga cagcaagaga
     2341 agtgacaaag gtcactgatg ataggccaca agtgtagata aagataagaa aacaatgtta
     2401 tttcagtgac tgttgactta ggcagctaat ctagactatt aaagtcaaat cctctctggg
     2461 aaaagttatc tttctgacaa tgcaaagtat gtttatttaa atatcagagt atcttcaatc
     2521 tgagaattaa agcatatgaa agcaatcttt cctaactccc tacgattcat cttataagta
     2581 aatttataag taaattttta aactacacta gtcttttaaa tatatataga aaaatatata
     2641 tacacacgta tagacatgta tatgtaatct atctacatat atataaaaaa tgcattttcc
     2701 aaatgcatta gcctgattgg actgtgccac agattagtgt cctcctatga aattttgagt
     2761 atgtcatttg taaatgttag cattataaat tgcaggagaa tttaagaatt gacttgtatt
     2821 tcactggata ctatagtacc cggtaagtta cacacctgct tgaccacata ccattcatgt
     2881 tacatgacac aaaataggaa aaaatctttt gctggtattg cgttcccttc tctcttagag
     2941 tcaaaaggat ctattctgca aaattaatca aaatattcaa aaggaaggca ggtttttcaa
     3001 aactgggcta agcaattatc aaactgaact aacctgcttt cagatatcct gttgtattgc
     3061 ccggtaagta tagtcacact tgtccaaccc gtgtctcaat atggaattca gaaaagttac
     3121 catatataaa cccagctata aatggttgta gaaaatataa gtatcagtgg gtttcctatg
     3181 taatagtgtt taaaacacaa atatttcaac catttggcaa ggtatatgtt gaagttcaca
     3241 agcgctggtc ctaaccagat cagataaagt ggattatgtt tctgatatgt acctaattat
     3301 tttgactata ctataatttt acaatatgca tattgaccgt atgttttaat aacacattcc
     3361 attgaaatga ccaagtagaa tttctcagtt acatggggca ggccgggcgc aatggctcat
     3421 gcctgtaatc ccaggacttt gggaggccaa ggcaggcaga tcacctgagg tcaggagttc
     3481 aagaccagcc tggccaacat ggcgaaaccc cgtctctact aaaaatacaa aaattagccg
     3541 ggcatggtag gtgggcgcct gtaatcccag ctactcagca ggctgaggca ggagaatcac
     3601 ttgaacctgg gaggcggagg ttgcagtgag ctgagattgt gccactgcac tccagcctag
     3661 gcaacagagc gagactctta tctcaaaaaa aaaaaaaaaa gtatttacag aaatgtacat
     3721 gctatggtgt tagagcatga atgtcaaatc cagggtctgt cagattcagt agtctcatct
     3781 acaaaataaa ccttgttttc cttctgtcct actcagttcc attttaagac taacaacaag
     3841 tttgaaaact tagttttata ttgctatgca gctaaacagc tgtgtaaaaa atggctgata
     3901 attactgtta tgtacaaaag agaaacttat tcaaggatgg tgaatagcaa caactaacct
     3961 ttttatcact ctgtaatgtg cagggtactt tatgcacaca tattacacaa gtaccttgca
     4021 tattatatat gcatcattat atatattacc atatatataa agtaacttgt acatttttga
     4081 ggttctatag aaatatataa tctcattaat tcttatgttc tcatgtttca acaggtactg
     4141 ttattctcat tttataaata aactcagaag ctcagtca
//
